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Viral Diagnostics

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https://www.readbyqxmd.com/read/28915927/a-decade-of-research-into-classical-swine-fever-marker-vaccine-cp7_e2alf-suvaxyn-%C3%A2-csf-marker-a-review-of-vaccine-properties
#1
REVIEW
Sandra Blome, Kerstin Wernike, Ilona Reimann, Patricia König, Claudia Moß, Martin Beer
Due to its impact on animal health and pig industry, classical swine fever (CSF) is still one of the most important viral diseases of pigs. To control the disease, safe and highly efficacious live attenuated vaccines exist for decades. However, until recently, the available live vaccines did not allow a serological marker concept that is essentially important to circumvent long-term trade restrictions. In 2014, a new live attenuated marker vaccine, Suvaxyn(®) CSF Marker (Zoetis), was licensed by the European Medicines Agency...
September 15, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28915908/advances-in-paediatrics-in-2016-current-practices-and-challenges-in-allergy-autoimmune-diseases-cardiology-endocrinology-gastroenterology-infectious-diseases-neonatology-nephrology-neurology-nutrition-pulmonology
#2
REVIEW
Carlo Caffarelli, Francesca Santamaria, Dora Di Mauro, Carla Mastrorilli, Silvia Montella, Sergio Bernasconi
This review reports main progresses in various pediatric issues published in Italian Journal of Pediatrics and in international journals in 2016. New insights in clinical features or complications of several disorders may be useful for our better understanding. They comprise severe asthma, changing features of lupus erythematosus from birth to adolescence, celiac disease, functional gastrointestinal disorders, Moebius syndrome, recurrent pneumonia. Risk factors for congenital heart defects, Kawasaki disease have been widely investigated...
September 16, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28915317/the-theoretical-reliability-of-pcr-based-fish-viral-diagnostic-methods-is-critically-affected-when-they-are-applied-to-fish-populations-with-low-prevalence-and-virus-loads
#3
C P Dopazo, P Moreno, J G Olveira, J J Borrego
AIMS: The reliability of PCR techniques is an important issue in viral diagnosis, and it is even crucial when they must be applied for detection of viruses in asymptomatic carriers. The problems will arise when the aim is to study fish wild populations, where the viral loads and prevalence values are extremely low. We have evaluated several PCR procedures employed by two laboratories for monitoring fish captured in several oceanographic campaigns performed in the Gulf of Cádiz. METHODS AND RESULTS: To evaluate the reliability of different diagnostics test used, we have re-analyzed fish samples that had been previously subjected to diagnosis for a surveillance of viruses performed in 2010-2011 in wild fish populations...
September 15, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28912184/inflammatory-cardiomyopathic-syndromes
#4
REVIEW
Barry H Trachtenberg, Joshua M Hare
Inflammatory activation occurs in nearly all forms of myocardial injury. In contrast, inflammatory cardiomyopathies refer to a diverse group of disorders in which inflammation of the heart (or myocarditis) is the proximate cause of myocardial dysfunction, causing injury that can range from a fully recoverable syndrome to one that leads to chronic remodeling and dilated cardiomyopathy. The most common cause of inflammatory cardiomyopathies in developed countries is lymphocytic myocarditis most commonly caused by a viral pathogenesis...
September 15, 2017: Circulation Research
https://www.readbyqxmd.com/read/28910938/-comparison-of-commercial-hiv-1-viral-load-tests-by-using-proficiency-test-results-in-china-2013-2015
#5
L Zhang, C Jin, Z Jiang, T Tang, Y Jiang, P L Pan
Objective: To compare the bio-equivalence among commercial HIV-1 viral load tests, including EasyQ HIV-1 v2.0 (EasyQ) from bioMerieux NucliSens of France; VERSANT HIV-1 RNA 3.0 assay (bDNA) from Siemens Healthcare Diagnostics of USA; COBAS AmpliPrep/COBAS TaqMan HIV-1 test (Taqman) from Roche Molecular Diagnosis of USA; Abbott Real Time HIV-1 Kit (M2000) from Abbott Molecular of USA and two domestic HIV-1 viral load test kits (domestic kit) from DaAn Gene Company of Sun Yat-Sen University and Liaoning Bio-Pharmaceutical company of Northeast pharmaceutical group, by using proficiency test results in China from 2013 to 2015...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28910866/an-aptamer-based-electrochemical-sensor-that-can-distinguish-influenza-virus-subtype-h1-from-h5
#6
Jin-Moo Lee, JunWon Kim, Ilhwan Ryu, Hye-Min Woo, Tae Gyun Lee, Woong Jung, Sanggyu Yim, Yong-Joo Jeong
The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype...
September 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28905952/detection-of-membrane-bound-and-soluble-antigens-by-magnetic-levitation
#7
Mikkel Schou Andersen, Emily Howard, Shulin Lu, Matthew Richard, Mark Gregory, Gordon Ogembo, Ofer Mazor, Pavel Gorelik, Nathan I Shapiro, Anish V Sharda, Ionita Ghiran
Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein-Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel...
September 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28905886/time-series-oligonucleotide-count-to-assign-antiviral-sirnas-with-long-utility-fit-in-the-big-data-era
#8
K Wada, Y Wada, Y Iwasaki, T Ikemura
Oligonucleotides are key elements of nucleic acid therapeutics such as small interfering RNAs (siRNAs). Influenza and Ebolaviruses are zoonotic RNA viruses mutating very rapidly, and their sequence changes must be characterized intensively to design therapeutic oligonucleotides with long utility. Focusing on a total of 182 experimentally validated siRNAs for influenza A, B and Ebolaviruses compiled by the siRNA database, we conducted time-series analyses of occurrences of siRNA targets in these viral genomes...
September 14, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28904430/immunohistochemical-analysis-of-anti-phospholipase-a2-receptor-antibody-on-renal-biopsies-a-single-tertiary-care-center-study
#9
A Gudipati, M S Uppin, R K Kalidindi, G Swarnalatha, U Das, G Taduri, S B Raju, L Rajasekhar, Aruna K Prayaga
Membranous nephropathy (MN) is one of the common cause of nephrotic syndrome. The discrimination between primary MN (iMN) and secondary MN is essential because of treatment implications. Immunohistochemical (IHC) evaluation with the help of anti-phospholipase A2 receptor (PLA2R) antibody helps in tissue evaluation of iMN, which is an easy, cost-effective, and pathologist-friendly technique. The study included 82 cases of MN over a period of 3 years. IHC using PLA2R antibody was performed on iMN and secondary cases with adequate tissue...
September 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/28904181/progress-and-challenges-towards-point-of-care-diagnostic-development-for-dengue
#10
Junxiong Pang, Po Ying Chia, David C Lye, Yee Sin Leo
Dengue detection strategies involve viral RNA, antigen and/or antibody detection. Each strategy has its advantages and disadvantages. Optimal, user-friendly and rapid diagnostic tests based on immunochromatographic assays are pragmatic point-of-care-test (POCT) in dengue endemic regions where there are limited laboratory capabilities and optimal storage conditions. Increasingly, there is a greater public health significance for multiplexing assay to differentiate dengue from Zika or pathogens with similar clinical presentations...
September 13, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28903865/economic-evaluation-of-the-eradication-program-for-bovine-viral-diarrhea-in-the-swiss-dairy-sector
#11
B Thomann, A Tschopp, I Magouras, M Meylan, G Schüpbach-Regula, B Häsler
Since 2008, the Swiss veterinary service has been running a mandatory eradication program for Bovine Viral Diarrhea (BVD) that is focused on detecting and eliminating persistently infected (PI) animals. Detection was initially based on antigen testing from ear tag samples of the entire cattle population, followed by antigen testing of all newborn calves until 2012. Since then, bulk milk serology (dairy herds) and blood sample serology (beef herds) have been used for the surveillance of disease-free herds. From 2008 to 2012, the proportion of newborn PI calves decreased from 1...
September 15, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28894729/procalcitonin-for-diagnostics-and-treatment-decisions-in-pediatric-lower-respiratory-tract-infections
#12
Philipp Baumann, Gurli Baer, Jessica Bonhoeffer, Aline Fuchs, Verena Gotta, Ulrich Heininger, Nicole Ritz, Gabor Szinnai, Jan Bonhoeffer
Mortality and morbidity remain high in pediatric lower respiratory tract infections (LRTIs) despite progress in research and implementation of global diagnostic and treatment strategies in the last decade. Still, 120 million annual episodes of pneumonia affect children younger than 5 years each year leading to 1.3 million fatalities with the major burden of disease carried by low- and middle-income countries (95%). The definition of pneumonia is still challenging. Traditional diagnostic measures (i.e., chest radiographs, C-reactive protein) are unable to distinguish viral and from bacterial etiology...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/28893022/correlation-of-vitamin-d3-levels-and-scorad-index-in-atopic-dermatits-a-case-control-study
#13
Saurabh Sharma, Tejinder Kaur, Suresh Kumar Malhotra, Jaswant Rai, Sunil Chaudhari
INTRODUCTION: Atopic Dermatitis (AD) is a common chronic inflammatory condition characterized clinically by pruritus and eczematous lesions. An inverse relationship has been suggested between serum 25-hydroxyvitamin D concentration and severity of atopic dermatitis. AIM: We carried out this controlled cross-sectional study to evaluate the association between the serum vitamin D3 levels and SCORAD index. MATERIALS AND METHODS: For this study, 40 patients with clinical diagnosis of AD based on UK diagnostic criteria were enrolled and 40 patients with minor ailments like superficial bacterial, fungal or viral infections and not suffering from atopic dermatitis were taken as controls...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892896/detection-of-cytomegalovirus-in-bronchoalveolar-lavage-fluid-from-hiv-positive-individuals-with-community-acquired-pneumonia
#14
Arati Mane, Pankaj Gujar, Shraddha Gaikwad, Tilak Dhamgaye, Arun Risbud
INTRODUCTION: Cytomegalovirus (CMV) pneumonia is one of the frequent viral pneumonia reported in persons with HIV infection. Knowledge of pulmonary CMV infection is important for deciding appropriate diagnostic strategies. However, there is scanty literature addressing the role of CMV aetiology among HIV positive individuals presenting with Community Acquired Pneumonia (CAP) using Bronchoalveolar Lavage (BAL) samples from India. AIM: To detect CMV in BAL fluid from HIV-positive individuals presenting with CAP...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28892764/a-european-multicientre-study-on-the-comparison-of-hbv-viral-loads-between-veris-hbv-assay-and-roche-cobas-%C3%A2-taqman-%C3%A2-hbv-test-abbott-realtime-hbv-assay-siemens-versant-hbv-assay-and-qiagen-artus-hbv-rg-kit
#15
Patrick Braun, Rafael Delgado, Monica Drago, Diana Fanti, Hervé Fleury, Jacques Izopet, Alessandra Lombardi, MaAngeles Marcos, Karine Sauné, Siobhan O'Shea, Alfredo Pérez-Rivilla, John Ramble, Pascale Trimoulet, Jordi Vila, Duncan Whittaker, Alain Artus, Daniel Rhodes
BACKGROUND: Hepatitis B viral load testing is essential to treatment and monitoring decisions in patients with chronic Hepatitis B. Beckman Coulter has developed the VERIS HBV Assay (Veris) for use on the fully automated DxN VERIS Molecular Diagnostics System.(1) OBJECTIVES: To evaluate the clinical performance of the Veris HBV Assay at multiple EU laboratories STUDY DESIGN: Method comparison was performed with a total of 344 plasma specimens from HBV infected patients tested with Veris and COBAS(®) TaqMan(®) HBV Test (Cobas), 207 specimens tested with Veris and RealTime HBV Assay (RealTime), 86 specimens tested with Veris and VERSANT(®) HBV Assay (Versant), and 74 specimens tested with Veris and artus(®) HBV RG PCR kit (artus)...
September 8, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28888629/prevalence-of-lapine-rotavirus-astrovirus-and-hepatitis-e-virus-in-canadian-domestic-rabbit-populations
#16
XiaoTing Xie, Joanna Bil, Emily Shantz, Jutta Hammermueller, Eva Nagy, Patricia V Turner
Lapine rotavirus and astrovirus have been associated with disease in rabbits, and there is strong evidence of zoonotic transmission of lapine hepatitis E virus (HEV). Outbreaks of enteritis are common on commercial meat farms, resulting in poor welfare, high rabbit mortality, and significant financial losses for rabbit producers. Currently, none of these viruses are routinely tested by diagnostic laboratories. In this study, we assessed the prevalence of rotavirus, astrovirus, and HEV RNA in 205 pooled and individual fecal samples from healthy Canadian laboratory, companion, shelter and commercial meat rabbit populations...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28888628/neuropathological-survey-reveals-underestimation-of-the-prevalence-of-neuroinfectious-diseases-in-cattle-in-switzerland
#17
Laura Truchet, Julia Walland, Daniel Wüthrich, Céline L Boujon, Horst Posthaus, Rémy Bruggmann, Gertraud Schüpbach-Regula, Anna Oevermann, Torsten Seuberlich
Neuroinfectious diseases in livestock represent a severe threat to animal health, but their prevalence is not well documented and the etiology of disease often remains unidentified. The aims of this study were to generate baseline data on the prevalence of neuroinfectious diseases in cattle in Switzerland by neuropathological survey, and to identify disease-associated pathogens. The survey was performed over a 1-year period using a representative number of brainstem samples (n=1816) from fallen cattle. In total, 4% (n=73) of the animals had significant lesions, the most frequent types of which were indicative of viral (n=27) and bacterial (n=31) etiologies...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28885940/collaborative-systems-biology-projects-for-the-military-medical-community
#18
Jeffrey J Zalatoris, Julia B Scheerer, Frank J Lebeda
INTRODUCTION: This pilot study was conducted to examine, for the first time, the ongoing systems biology research and development projects within the laboratories and centers of the U.S. Army Medical Research and Materiel Command (USAMRMC). The analysis has provided an understanding of the breadth of systems biology activities, resources, and collaborations across all USAMRMC subordinate laboratories. METHODS: The Systems Biology Collaboration Center at USAMRMC issued a survey regarding systems biology research projects to the eight U...
September 2017: Military Medicine
https://www.readbyqxmd.com/read/28885354/acute-versus-subacute-community-acquired-meningitis-analysis-of-611-patients
#19
COMPARATIVE STUDY
Tarek Sulaiman, Lucrecia Salazar, Rodrigo Hasbun
Community-acquired meningitis can be classified into acute and subacute presentations by the duration of illness of ≤ or >5 days, respectively. There are currently no studies comparing the clinical features, management decisions, etiologies, and outcomes between acute and subacute presentations.It is a retrospective study of adults with community-acquired meningitis hospitalized in Houston, TX between January 2005 and January 2010. An adverse clinical outcome was defined as a Glasgow Outcome Scale score of ≤4...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28883869/ataxia-in-childhood-epidemiological-clinical-and-neuroradiologic-features-and-the-risk-of-recurrence
#20
REVIEW
Mohsen Javadzadeh, Masoud Hassanvand Amouzadeh, Shaghayegh Sadat Esmail Nejad, Ezatollah Abasi, Abbas Alipour, Mohsen Mollamohammadi
OBJECTIVE: This study was conducted on the demographic data, clinical characteristics, electroencephalography, neuroradiological findings, and their impact on the recurrence of ataxia. MATERIALS & METHODS: A 3-yr retrospective review of 49 children with ataxia in Mofid Children Hospital, Tehran, Iran was conducted from Apr 2013 to Apr 2016. The demographic, clinical and paraclinical data were recorded in pre-prepared questionnaires. The patients were also classified in two groups of with or without recurrence and the results were compared...
2017: Iranian Journal of Child Neurology
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