keyword
https://read.qxmd.com/read/38488962/transcriptional-levels-of-intercellular-junction-proteins-in-an-alveolar-epithelial-cell-line-exposed-to-irradiation-or-bleomycin
#21
JOURNAL ARTICLE
E S Karetnikova, N Jarzebska, R N Rodionov, P M Spieth, A G Markov
We performed a comparative study of the effects of X-ray irradiation and bleomycin on the mRNA levels of E-cadherin and tight junction proteins (claudin-3, claudin-4, claudin-18, ZO-2, and occludin) in an alveolar epithelial cell line L2. Irradiation decreased claudin-4 levels and increased occludin levels, while the levels of other mRNAs remained unchanged. Bleomycin increased the expression levels of all proteins examined except claudin-3. Irradiation and bleomycin have different effects on the expression level of intercellular junction proteins, indicating different reactions triggered in alveolar epithelial cells and a great prospects of further comparative studies...
March 15, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38488562/magnesium-l-threonate-treats-alzheimer-s-disease-by-modulating-the-microbiota-gut-brain-axis
#22
JOURNAL ARTICLE
Wang Liao, Jiana Wei, Chongxu Liu, Haoyu Luo, Yuting Ruan, Yingren Mai, Qun Yu, Zhiyu Cao, Jiaxin Xu, Dong Zheng, Zonghai Sheng, Xianju Zhou, Jun Liu
JOURNAL/nrgr/04.03/01300535-202410000-00029/figure1/v/2024-02-06T055622Z/r/image-tiff Disturbances in the microbiota-gut-brain axis may contribute to the development of Alzheimer's disease. Magnesium-L-threonate has recently been found to have protective effects on learning and memory in aged and Alzheimer's disease model mice. However, the effects of magnesium-L-threonate on the gut microbiota in Alzheimer's disease remain unknown. Previously, we reported that magnesium-L-threonate treatment improved cognition and reduced oxidative stress and inflammation in a double-transgenic line of Alzheimer's disease model mice expressing the amyloid-β precursor protein and mutant human presenilin 1 (APP/PS1)...
October 1, 2024: Neural Regeneration Research
https://read.qxmd.com/read/38484956/effect-and-mechanism-of-prunella-vulgaris-l-extract-on-alleviating-lipopolysaccharide-induced-acute-mastitis-in-protecting-the-blood-milk-barrier-and-reducing-inflammation
#23
JOURNAL ARTICLE
Hongshan Luo, Yamei Li, Jingchen Xie, Chunfang Xu, Zhimin Zhang, Minjie Li, Bohou Xia, Zhe Shi, Limei Lin
ETHNOPHARMACOLOGICAL RELEVANCE: According to ancient literature, Prunella vulgaris L. (P vulgaris) alleviates mastitis and has been used in China for many years; however, there are no relevant reports that confirm this or the mechanism of its efficacy. AIM OF THE STUDY: To explore the anti-acute mastitis effect and potential mechanism of P vulgaris extract. MATERIALS AND METHODS: First, the active ingredients and targets of P vulgaris against mastitis were predicted using network pharmacology...
March 12, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38484487/effect-of-high-sodium-intake-on-gut-tight-junctions-structure-and-permeability-to-bacterial-toxins-in-a-rat-model-of-chronic-kidney-disease
#24
JOURNAL ARTICLE
María de la Luz Villela-Torres, María-Del-Carmen Prado-Uribe, Marcela Ávila Díaz, Héctor Quezada Pablo, Elizabeth Soria-Castro, Nuria Esturau Escofet, Catalina Elizabeth Flores Maldonado, Ramón Paniagua
INTRODUCTION: Uremic toxicity changes the gut structure and permeability, allowing bacterial toxins to translocate from the lumen to the blood during chronic kidney failure (CKD). Clinical fluid overload and tissue edema without uremia have similar effects but have not been adequately demonstrated and analyzed in CKD. AIMS: To investigate the effect of sodium intake on the plasma concentration of gut-derived uremic toxins, indoxyl sulfate (IS), and p-cresyl sulfate (pCS) and the expression of genes and proteins of epithelial gut tight junctions in a rat model of CKD...
March 13, 2024: Archives of Medical Research
https://read.qxmd.com/read/38476896/antibiotic-administration-aggravates-asthma-by-disrupting-gut-microbiota-and-the-intestinal-mucosal-barrier-in-an-asthma-mouse-model
#25
JOURNAL ARTICLE
Cheng-Ling Xu, Cui Wang, Gao-Bin Li, Tong Zhao, Rui-Ling Zhou, Jing Chen
In humans, gut microbiota can determine the health status. The regulatory mechanisms of the gut microbiota in asthma must be elucidated. Although antibiotics (ABXs) can clear infections, they markedly alter the composition and abundance of gut microbiota. The present study used ABX-treated mice to examine the time-dependent effects of ABX administration on the gut microbiota and intestinal mucosal barrier. The mouse asthma model was established using ovalbumin (OVA) and gavaged with an ABX cocktail for different durations (1 or 2 weeks) and stacked sequences...
April 2024: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/38474310/early-increase-in-blood-brain-barrier-permeability-in-a-murine-model-exposed-to-fifteen-days-of-intermittent-hypoxia
#26
JOURNAL ARTICLE
Frederic Roche, Anne Briançon-Marjollet, Maurice Dematteis, Marie Baldazza, Brigitte Gonthier, Frederique Bertholon, Nathalie Perek, Jean-Louis Pépin
Obstructive sleep apnea (OSA) is characterized by intermittent repeated episodes of hypoxia-reoxygenation. OSA is associated with cerebrovascular consequences. An enhanced blood-brain barrier (BBB) permeability has been proposed as a marker of those disorders. We studied in mice the effects of 1 day and 15 days intermittent hypoxia (IH) exposure on BBB function. We focused on the dorsal part of the hippocampus and attempted to identify the molecular mechanisms by combining in vivo BBB permeability (Evans blue tests) and mRNA expression of several junction proteins (zona occludens (ZO-1,2,3), VE-cadherin, claudins (1,5,12), cingulin) and of aquaporins (1,4,9) on hippocampal brain tissues...
March 6, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473876/fermented-protaetia-brevitarsis-larvae-improves-neurotoxicity-in-chronic-ethanol-induced-dementia-mice-via-suppressing-akt-and-nf-%C3%AE%C2%BAb-signaling-pathway
#27
JOURNAL ARTICLE
Hyo Lim Lee, Jong Min Kim, Min Ji Go, Han Su Lee, Ju Hui Kim, Ho Jin Heo
This study was investigated to examine the neuroprotective effect of fermented Protaetia brevitarsis larvae (FPB) in ethanol-induced-dementia mice. Consumption of FPB by mice resulted in improved memory dysfunction in the Y-maze, passive avoidance, and Morris water maze tests. FPB significantly decreased oxidative stress by regulating levels of malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) in brain tissues. In addition, FPB restored cerebral mitochondrial dysfunction by modulating levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and ATP...
February 23, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473701/the-role-of-zo-2-in-modulating-jam-a-and-%C3%AE-actin-junctional-recruitment-apical-membrane-and-tight-junction-tension-and-cell-response-to-substrate-stiffness-and-topography
#28
JOURNAL ARTICLE
Diana Cristina Pinto-Dueñas, Christian Hernández-Guzmán, Patrick Matthew Marsch, Anand Sunil Wadurkar, Dolores Martín-Tapia, Lourdes Alarcón, Genaro Vázquez-Victorio, Juan Vicente Méndez-Méndez, José Jorge Chanona-Pérez, Shikha Nangia, Lorenza González-Mariscal
This work analyzes the role of the tight junction (TJ) protein ZO-2 on mechanosensation. We found that the lack of ZO-2 reduced apical membrane rigidity measured with atomic force microscopy, inhibited the association of γ-actin and JAM-A to the cell border, and instead facilitated p114RhoGEF and afadin accumulation at the junction, leading to an enhanced mechanical tension at the TJ measured by FRET, with a ZO-1 tension probe, and increased tricellular TJ tension. Simultaneously, adherens junction tension measured with an E-cadherin probe was unaltered...
February 20, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473179/ahr-activation-ameliorates-intestinal-barrier-damage-in-immunostressed-piglets-by-regulating-intestinal-flora-and-its-metabolism
#29
JOURNAL ARTICLE
Xiaomei Wu, Yalei Zhang, Mengyao Ji, Wen Yang, Tanjie Deng, Guanyu Hou, Liguang Shi, Wenjuan Xun
The primary factor leading to elevated rates of diarrhea and decreased performance in piglets is immunological stress. The regulation of immune stress through the intestinal flora is a crucial mechanism to consider. In total, 30 weaned piglets were randomly allocated to five groups: the basal diet group (Control), basal diet + lipopolysaccharides group (LPS), basal diet + 250 μg/kg 6-Formylindolo [3,2-b] carbazole + LPS group (FICZ), basal diet + 3mg/kg Cardamonin + LPS group (LCDN), and basal diet + 6mg/kg Cardamonin + LPS group (HCDN/CDN)...
March 4, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38470332/improving-stability-of-human-three-dimensional-skin-equivalents-using-plasma-surface-treatment
#30
JOURNAL ARTICLE
Suphanun Phuphanitcharoenkun, Fiona Louis, Yoshihiro Sowa, Michiya Matsusaki, Tanapat Palaga
In developing three-dimensional (3D) human skin equivalents (HSEs), preventing dermis and epidermis layer distortion due to the contraction of hydrogels by fibroblasts is a challenging issue. Previously, a fabrication method of HSEs was tested using a modified solid scaffold or a hydrogel matrix in combination with the natural polymer coated onto the tissue culture surface, but the obtained HSEs exhibited skin layer contraction and loss of the skin integrity and barrier functions. In this study, we investigated the method of HSE fabrication that enhances the stability of the skin model by using surface plasma treatment...
March 12, 2024: Biotechnology and Bioengineering
https://read.qxmd.com/read/38460644/fxr1-stabilizes-snord63-to-regulate-blood-tumor-barrier-permeability-through-snord63-mediated-2-o-methylation-of-pou6f1
#31
JOURNAL ARTICLE
Chanchan Liang, Bei Zhai, Deng Wei, Ben Niu, Jun Ma, Yilong Yao, Yang Lin, Yunhui Liu, Xiaobai Liu, Ping Wang
How selectively increase blood-tumor barrier (BTB) permeability is crucial to enhance the delivery of chemotherapeutic agents to brain tumor tissues. In this study, we established in vitro models of the blood-brain barrier (BBB) and BTB using endothelial cells (ECs) co-cultured with human astrocytes (AECs) and glioma cells (GECs), respectively. The findings revealed high expressions of the RNA-binding protein FXR1 and SNORD63 in GECs, where FXR1 was found to bind and stabilize SNORD63. Knockdown of FXR1 resulted in decreased expression of tight-junction-related proteins and increased BTB permeability by down-regulating SNORD63...
March 7, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38452844/cerebral-endothelial-cells-mediated-enhancement-of-brain-pericyte-number-and-migration-in-oxygen-glucose-deprivation-involves-the-hif-1%C3%AE-pdgf-%C3%AE-signaling
#32
JOURNAL ARTICLE
Shi-Na Song, Wen-Ping Dong, Xin-Xin Dong, Fang Guo, Lin Ren, Chang-Xin Li, Jian-Ming Wang
The present study focused on whether hypoxia-inducible factor-1alpha (HIF-1α) and platelet-derived factor-beta (PDGF-β) are involved in the crosstalk between brain microvascular endothelial cells (BMECs) and brain vascular pericytes (BVPs) under ischaemic-hypoxic conditions. Mono-cultures or co-cultures of BVPs and BMECs were made for the construction of the blood-brain barrier (BBB) model in vitro and then exposed to control and oxygen-glucose deprivation (OGD) conditions. BBB injury was determined by assessing the ability, apoptosis, and migration of BVPs and the transendothelial electrical resistance and horseradish peroxidase permeation of BMECs...
March 5, 2024: Brain Research
https://read.qxmd.com/read/38451226/-bacillus-halotolerans-sw207-alleviates-enterotoxigenic-escherichia-coli-induced-inflammatory-responses-in-weaned-piglets-by-modulating-the-intestinal-epithelial-barrier-the-tlr4-myd88-nf-%C3%AE%C2%BAb-pathway-and-intestinal-microbiota
#33
JOURNAL ARTICLE
Minghan Li, Dongyu Zhao, Jialin Guo, Tianxu Pan, Tianming Niu, Yanqi Jiang, Chunwei Shi, Haibin Huang, Nan Wang, Di Zhang, Shumin Zhang, Chunfeng Wang, Guilian Yang
Enterotoxigenic Escherichia coli (ETEC) is one of the major pathogens contributing to piglet diarrhea, with significant implications for both piglet health and the economic aspects of the livestock industry. SW207 is an isolate of Bacillus halotolerans isolated from the cold- and disease-resistant Leixiang pigs in Northeastern China. We have discovered that SW207 can survive in the pig's gastrointestinal fluid and under conditions of high bile salt concentration, displaying potent antagonistic activity against ETEC...
March 7, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38448724/andrographolide-improves-apoe4-mediated-blood-brain-barrier-injury-by-alleviating-inflammation
#34
JOURNAL ARTICLE
Xuebin Zhou, Jinhua Li, Shengli Quan, Xinyue Zhang, Lili Gu, Min Hu, Wenhai Huang, Qin Li
The pathological and physiological studies of Alzheimer's disease (AD) have been in-depth, and apolipoprotein E4 (ApoE4) has been proven to be highly correlated with AD, and clinical and experimental data show that ApoE4 can cause blood-brain barrier (BBB) injury, and the change of BBB permeability is an important factor affecting the development of AD. Andrographolide (Andro), as the active component of the natural plant Andrographis paniculata, has been proven to have anti-inflammatory and antioxidant effects, which have potential neuroprotective effects...
March 6, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38448341/calcitriol-modulates-epidermal-tight-junction-barrier-function-in-human-keratinocytes
#35
JOURNAL ARTICLE
Juan Valentin Trujillo-Paez, Ge Peng, Hai Le Thanh Nguyen, Masahiro Nakamura, Yoshie Umehara, Hainan Yue, Risa Ikutama, Miho Takahashi, Shigaku Ikeda, Hideoki Ogawa, Ko Okumura, François Niyonsaba
BACKGROUND: The aberrant expression of tight junction (TJ) proteins play an important role in several diseases with impaired skin barriers, including atopic dermatitis, psoriasis, and chronic wounds. The evidence provided thus far suggests an important role of calcitriol in skin homeostasis. However, it is not known whether calcitriol improves the impaired skin barrier. OBJECTIVE: To investigate the effect of calcitriol on TJ barrier function in human primary keratinocytes...
February 7, 2024: Journal of Dermatological Science
https://read.qxmd.com/read/38447056/a-comparative-analysis-of-git-barrier-function-and-immune-markers-in-gilt-vs-sow-progeny-at-birth-and-weaning
#36
JOURNAL ARTICLE
Udani A Wijesiriwardana, John R Pluske, Jessica R Craig, John B Furness, Mitchell Ringuet, Linda J Fothergill, Frank R Dunshea, Jeremy J Cottrell
Progeny born to primiparous sows (gilt progeny; GP) have lower birth, weaning and slaughter weights than sow progeny (SP). GP also have reduced gastrointestinal tract (GIT) development, as evidenced by lower organ weights. Therefore, the aim of this experiment was to quantify changes in GIT barrier function that occur birth and weaning, representing two major challenges to the young piglet. The effects of parity (GP vs SP) in GIT barrier integrity function was quantified at four timepoints: birth (~0 h), 24 hours after birth (24 h), 1-day pre-weaning (PrW) and 1-day post-weaning (PoW) in commercially reared piglets...
March 6, 2024: Journal of Animal Science
https://read.qxmd.com/read/38446668/mosaic-deletion-of-claudin-5-reveals-rapid-non-cell-autonomous-consequences-of-blood-brain-barrier-leakage
#37
JOURNAL ARTICLE
Elisa Vázquez-Liébanas, Giuseppe Mocci, Weihan Li, Bàrbara Laviña, Avril Reddy, Claire O'Connor, Natalie Hudson, Zaher Elbeck, Ioannis Nikoloudis, Konstantin Gaengel, Michael Vanlandewijck, Matthew Campbell, Christer Betsholtz, Maarja Andaloussi Mäe
Claudin-5 (CLDN5) is an endothelial tight junction protein essential for blood-brain barrier (BBB) formation. Abnormal CLDN5 expression is common in brain disease, and knockdown of Cldn5 at the BBB has been proposed to facilitate drug delivery to the brain. To study the consequences of CLDN5 loss in the mature brain, we induced mosaic endothelial-specific Cldn5 gene ablation in adult mice (Cldn5iECKO ). These mice displayed increased BBB permeability to tracers up to 10 kDa in size from 6 days post induction (dpi) and ensuing lethality from 10 dpi...
March 5, 2024: Cell Reports
https://read.qxmd.com/read/38443177/-protective-effects-of-galacto-oligosaccharide-and-polydextrose-on-caco-2-intestinal-cell-barrier-injury-model
#38
JOURNAL ARTICLE
Na Li, Wanqing Guo, Rong Xiang, Xuemei Zhu, Mengqi Yu, Jingyu Hao, Sufang Duan, Lishi Zhang, Ya Yuan, Jinyao Chen
OBJECTIVE: To explore the protective effect of different ratios of galactose oligosaccharide(GOS) and polydextrose(PDX) on intestinal cell barrier damage model of Caco-2. METHODS: The same batch of Caco-2 cells were cultured to form a cell barrier model and randomly divided into damaged model group without calcium, calcium-containing blank control group(1.8 mmol/L Ca~(2+)), low-ratio/low-dose group(1.8 mmol/L Ca~(2+)+2 mg/mL GOS+2 mg/mL PDX) and low-ratio/medium-dose group(1...
January 2024: Wei Sheng Yan Jiu, Journal of Hygiene Research
https://read.qxmd.com/read/38442786/mitigation-effect-of-hesperidin-on-x-ray-radiation-induced-intestinal-barrier-dysfunction-in-caco-2%C3%A2-cell-monolayers
#39
JOURNAL ARTICLE
Ha-Young Park, Jin-Hee Yu
The tight junctions (TJs) and barrier function of the intestinal epithelium are highly sensitive to radiation. However, polyphenols can be used to reverse the effects of radiation. Here, we investigated the effects of hesperidin (hesperetin-7-rhamnoglucoside) on X-ray-induced intestinal barrier dysfunction in human epithelial Caco-2 monolayers. To examine whether hesperidin mitigated the effects of X-ray exposure (2 Gy), cell survival was evaluated and intestinal barrier function was assessed by measuring the transepithelial flux, apparent permeability coefficient (Papp ), and barrier integrity...
March 3, 2024: Food and Chemical Toxicology
https://read.qxmd.com/read/38442546/d-beta-hydroxybutyrate-up-regulates-claudin-1-and-alleviates-the-intestinal-hyperpermeability-in-lipopolysaccharide-treated-mice
#40
JOURNAL ARTICLE
Ting Wang, Yuchen Zhuang, Chenglong Yu, Zhaobo Wang, Yuan Liu, Qian Xu, Kun Liu, Yanning Li
The hyperpermeability of intestinal epithelium is a key contributor to the occurrence and development of systemic inflammation. Although D-beta-hydroxybutyrate (BHB) exhibits various protective effects, whether it affects the permeability of intestinal epithelium in systemic inflammation has not been clarified. In this study, we investigated the effects of BHB on the intestinal epithelial permeability, the epithelial marker E-cadherin and the tight junction protein Claudin-1 in colon in the lipopolysaccharide (LPS)-induced systemic inflammation mouse model...
March 1, 2024: Tissue & Cell
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