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Tight junctions claudin

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https://www.readbyqxmd.com/read/28730076/scutellaria-barbata-attenuates-diabetic-retinopathy-by-preventing-retinal-inflammation-and-the-decreased-expression-of-tight-junction-protein
#1
Xi-Yu Mei, Ling-Yu Zhou, Tian-Yu Zhang, Bin Lu, Li-Li Ji
AIM: To observe the attenuation of ethanol extract of Herba Scutellaria barbata (SE) against diabetic retinopathy (DR) and its engaged mechanism. METHODS: C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ, 55 mg/kg) for 5 consecutive days to induce diabetes. The diabetic mice were orally given with SE (100, 200 mg/kg) for 1mo at 1mo after STZ injection. Blood-retinal barrier (BRB) breakdown was detected by using Evans blue permeation assay. Real-time polymerase chain reaction (RT-PCR), Western blot and immunofluorescence staining were used to detect mRNA and protein expression...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28719888/inhibition-of-single-routes-of-intracellular-signaling-is-not-sufficient-to-neutralize-the-biphasic-disturbance-of-a-retinal-endothelial-cell-barrier-induced-by-vegf-a165
#2
Heidrun L Deissler, Gerhard K Lang, Gabriele E Lang
BACKGROUND/AIMS: Hallmark of diabetic macular edema is the enhanced permeability of retinal endothelial cells (REC) induced by vascular endothelial growth factor (VEGF-A165), which acts through activating specific receptors. To improve the predictability of inhibitors' potentials to block harmful effects of VEGF-A165, we investigated if its signaling pathways triggered in REC are redundant. METHODS: Immortalized bovine REC monolayers were treated with inhibitors specific for various protein kinases in combination with VEGF-A165...
July 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28715583/tnf%C3%AE-induced-disruption-of-the-blood-retinal-barrier-in-vitro-is-regulated-by-intracellular-3-5-cyclic-adenosine-monophosphate-levels
#3
Anne-Eva van der Wijk, Ilse M C Vogels, Cornelis J F van Noorden, Ingeborg Klaassen, Reinier O Schlingemann
Purpose: Proinflammatory cytokines such as tumor necrosis factor (TNFα) may have a causative role in blood-retinal barrier (BRB) disruption, which is an essential step in the development of diabetic macular edema. The purpose of our study was to determine whether TNFα increases permeability in an in vitro model of the BRB and to explore the mechanisms involved. Methods: Primary bovine retinal endothelial cells (BRECs) were grown on Transwell inserts and cells were stimulated with TNFα or a combination of TNFα, IL1β, and VEGF...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28706505/cell-cell-interaction-proteins-gap-junctions-tight-junctions-and-desmosomes-and-water-transporter-aquaporin-4-in-meningothelial-cells-of-the-human-optic-nerve
#4
Thi Ngoc Co Zeleny, Corina Kohler, Albert Neutzner, Hanspeter E Killer, Peter Meyer
PURPOSE: Meningothelial cells (MECs) play a central role in the maintenance of cerebrospinal fluid (CSF) homeostasis and in physiological and pathophysiological processes within the subarachnoid space (SAS) linking them to optic nerve (ON) pathologies. Still, not much is known about their structural properties that might enable MECs to perform specific functions within the ON microenvironment. METHODS: For closer characterization of the structural properties of the human MEC layer in the arachnoid, we performed immunohistological analyses to evaluate the presence of cell-cell interaction markers, namely, markers for tight junctions (JAM1, Occludin, and Claudin 5), gap junctions (Connexin 26 and 43), and desmosomes (Desmoplakin) as well as for water channel marker aquaporin 4 (AQP4) in retrobulbar, midorbital, and intracanalicular human ON sections...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28705835/transendothelial-movement-of-adiponectin-is-restricted-by-glucocorticoids
#5
Thanh Q Dang, Nanyoung Yoon, Helen Chasiotis, Emily C Dunford, Qilong Feng, Pingnian He, Michael C Riddell, Scott P Kelly, Gary Sweeney
Altered permeability of the endothelial barrier in a variety of tissues has implications both in disease pathogenesis and treatment. Glucocorticoids are potent mediators of endothelial permeability, and this forms the basis for their heavily prescribed use as medications to treat ocular disease. However, the effect of glucocorticoids on endothelial barriers elsewhere in the body is less well studied. Here, we investigated glucocorticoid-mediated changes in endothelial flux of Adiponectin (Ad), a hormone with a critical role in diabetes...
August 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28701916/the-role-of-hotair-mir-148b-3p-usf1-on-regulating-the-permeability-of-btb
#6
Libo Sa, Yan Li, Lini Zhao, Yunhui Liu, Ping Wang, Libo Liu, Zhen Li, Jun Ma, Heng Cai, Yixue Xue
Homeobox transcript antisense intergenic RNA (HOTAIR), as a long non-coding RNA (lncRNA), has been considered to play critical roles in the biological properties of various tumors. The purposes of this study were to investigate the role and possible molecular mechanisms of HOTAIR in regulating the permeability of blood tumor barrier (BTB) in vitro. Our present study elucidated that the expressions of HOTAIR and upstream stimulatory factor 1 (USF1) was up-regulated, but miR-148b-3p was down-regulated in glioma microvascular endothelial cells (GECs)...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28699235/expression-patterns-of-claudins-in-patients-with-triple-negative-breast-cancer-are-associated-with-nodal-metastasis-and-worse-outcome
#7
Ayaka Katayama, Tadashi Handa, Kei Komatsu, Maria Togo, Jun Horiguchi, Masahiko Nishiyama, Tetsunari Oyama
Claudins (CLDNs) are key cell adhesion molecules, which compose tight junctions (TJs), and the disruption of TJs is associated with cancer development. Here we immunohistochemically studied expression patterns of CLDNs in 222 primary invasive breast cancers including 68 triple-negative breast cancers (TNBCs), and examined their correlation with epithelial-to-mesenchymal transition (EMT)-related markers, breast cancer stem cell (BCSC) markers, and clinicopathological features including patients' clinical outcome...
July 11, 2017: Pathology International
https://www.readbyqxmd.com/read/28699141/epi-perineural-and-schwann-cells-as-well-as-perineural-sheath-integrity-are-affected-following-2-4-d-exposure
#8
Marzieh Sharifi Pasandi, Farshad Hosseini Shirazi, Mohammad Reza Gholami, Hossein Salehi, Nowruz Najafzadeh, Mohammad Mazani, Hatef Ghasemi Hamidabadi, Ali Niapour
2,4-dicholorophenoxy acetic acid (2,4-D) is a worldwide-known hormone herbicide. However, there are increasing concerns about its exposure and risks of developing pathological conditions for the peripheral nervous system. The aim of this study was to investigate the mechanism(s) involved in the toxicity of 2,4-D on peripheral nerve's cellular components. The epi/perineural and Schwann cells and a total of three cell lines were treated with 2,4-D. The viability of cells at different doses of 2,4-D was measured by MTT assay...
July 11, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28698546/obesity-induces-organ-and-tissue-specific-tight-junction-restructuring-and-barrier-deregulation-by-claudin-switching
#9
Rizwan Ahmad, Bilal Rah, Dhundy Bastola, Punita Dhawan, Amar B Singh
Obesity increases susceptibility to multiple organ disorders, however, underlying mechanisms remain unclear. The subclinical inflammation assisted by obesity-induced gut permeability may underlie obesity-associated co-morbidities. Despite eminent clinical significance of the obesity led gut barrier abnormalities, its precise molecular regulation remains unclear. It is also unknown whether barrier deregulations, similar to the gut, characterize other vital organs in obese individuals. The claudin family of proteins is integral to the tight junction (TJ), the apical cell-cell adhesion and a key regulator of the epithelial barrier...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28690765/novel-therapeutic-effects-of-leonurine-on-ischemic-stroke-new-mechanisms-of-bbb-integrity
#10
Qiu-Yan Zhang, Zhi-Jun Wang, De-Miao Sun, Ying Wang, Peng Xu, Wei-Jun Wu, Xin-Hua Liu, Yi-Zhun Zhu
Stroke is a leading cause of morbidity and mortality globally. Leonurine (also named SCM-198), a compound extracted from Herba leonuri, was effective on the prevention of various cardiovascular and brain diseases. The purpose of this study was to explore the possible therapeutic potential of SCM-198 against ischemia reperfusion injury and underlying mechanisms. In the in vivo transient middle cerebral artery occlusion (tMCAO) rat model, we found that treatment with SCM-198 could decrease infarct volume and improve neurological deficit by protecting against blood-brain barrier (BBB) breakdown...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28686597/altered-paracellular-cation-permeability-due-to-a-rare-cldn10b-variant-causes-anhidrosis-and-kidney-damage
#11
Joakim Klar, Jörg Piontek, Susanne Milatz, Muhammad Tariq, Muhammad Jameel, Tilman Breiderhoff, Jens Schuster, Ambrin Fatima, Maria Asif, Muhammad Sher, Katrin Mäbert, Anja Fromm, Shahid M Baig, Dorothee Günzel, Niklas Dahl
Claudins constitute the major component of tight junctions and regulate paracellular permeability of epithelia. Claudin-10 occurs in two major isoforms that form paracellular channels with ion selectivity. We report on two families segregating an autosomal recessive disorder characterized by generalized anhidrosis, severe heat intolerance and mild kidney failure. All affected individuals carry a rare homozygous missense mutation c.144C>G, p.(N48K) specific for the claudin-10b isoform. Immunostaining of sweat glands from patients suggested that the disease is associated with reduced levels of claudin-10b in the plasma membranes and in canaliculi of the secretory portion...
July 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28681173/pro-apoptotic-effect-of-%C3%AE-2-tgz-in-claudin-1-low-triple-negative-breast-cancer-cells-involvement-of-claudin-1
#12
Geoffroy Marine, Kleinclauss Alexandra, Grandemange Stéphanie, Hupont Sébastien, Boisbrun Michel, Flament Stéphane, Grillier-Vuissoz Isabelle, Kuntz Sandra
PURPOSE: 40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tight junction. Patients with these "claudin-1-low" tumors present a higher relapse incidence. A major challenge in oncology is the development of innovative therapies for such poor prognosis tumors. In this context, we study the anticancer effects of ∆2-TGZ, a compound derived from troglitazone (TGZ), on cell models of these tumors. METHODS AND RESULTS: In MDA-MB-231 and Hs578T "claudin-1-low" TNBC cells, Δ2-TGZ treatment induced claudin-1 protein expression and triggered apoptosis as measured by FACS analysis (annexin V/PI co-staining)...
July 5, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28680530/mir-let7a-controls-the-cell-death-and-tight-junction-density-of-brain-endothelial-cells-under-high-glucose-condition
#13
Juhyun Song, So Ra Yoon, Oh Yoen Kim
Hyperglycemia-induced stress in the brain of patients with diabetes triggers the disruption of blood-brain barrier (BBB), leading to diverse neurological diseases including stroke and dementia. Recently, the role of microRNA becomes an interest in the research for deciphering the mechanism of brain endothelial cell damage under hyperglycemia. Therefore, we investigated whether mircoRNA Let7A (miR-Let7A) controls the damage of brain endothelial (bEnd.3) cells against high glucose condition. Cell viability, cell death marker expressions (p-53, Bax, and cleaved poly ADP-ribose polymerase), the loss of tight junction proteins (ZO-1 and claudin-5), proinflammatory response (interleukin-6, tumor necrosis factor-α), inducible nitric oxide synthase, and nitrite production were confirmed using MTT, reverse transcription-PCR, quantitative-PCR, Western blotting, immunofluorescence, and Griess reagent assay...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28679754/sec24c-dependent-transport-of-claudin-1-regulates-hepatitis-c-virus-entry
#14
Peiqi Yin, Ye Li, Leiliang Zhang
Claudin-1 is an HCV co-receptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little is known about how the level of claudin-1 in cell surface is regulated by host vesicular transport. Here, we identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the COPII vesicular transport system. By interacting with Sec24C through its C-terminal YV, claudin-1 is transported from the ER and is eventually targeted into cell surface. Blocking COPII transport inhibits HCV entry by reducing the level of claudin-1 in cell surface...
July 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28676351/bidirectional-brain-gut-interactions-and-chronic-pathological-changes-after-traumatic-brain-injury-in-mice
#15
Elise L Ma, Allen D Smith, Neemesh Desai, Lumei Cheung, Marie Hanscom, Bogdan A Stoica, David J Loane, Terez Shea-Donohue, Alan I Faden
OBJECTIVES: Traumatic brain injury (TBI) has complex effects on the gastrointestinal tract that are associated with TBI-related morbidity and mortality. We examined changes in mucosal barrier properties and enteric glial cell response in the gut after experimental TBI in mice, as well as effects of the enteric pathogen Citrobacter rodentium (Cr) on both gut and brain after injury. METHODS: Moderate-level TBI was induced in C57BL/6 mice by controlled cortical impact (CCI)...
July 1, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28674421/chronic-stress-and-intestinal-barrier-dysfunction-glucocorticoid-receptor-and-transcription-repressor-hes1-regulate-tight-junction-protein-claudin-1-promoter
#16
Gen Zheng, Gordon Victor Fon, Walter Meixner, Amy Creekmore, Ye Zong, Michael K Dame, Justin Colacino, Priya H Dedhia, Shuangsong Hong, John W Wiley
Chronic stress and elevated glucocorticoid hormone are associated with decreases in the intestinal epithelial tight junction protein claudin-1 (CLDN1). Human/rat CLDN1 promoters contain glucocorticoid response elements (GREs) and adjacent transcription repressor HES1 binding N-boxes. Notch signaling target HES1 expression was high and glucocorticoid receptor (NR3C1) low at the crypt base and the pattern reversed at the crypt apex. Chronic stress reduced overall rat colon HES1 and NR3C1 that was associated with CLDN1 downregulation...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674042/a-novel-hypokalemic-alkalotic-salt-losing-tubulopathy-in-patients-with-cldn10-mutations
#17
Ernie M H F Bongers, Luke M Shelton, Susanne Milatz, Sjoerd Verkaart, Anneke P Bech, Jeroen Schoots, Elisabeth A M Cornelissen, Markus Bleich, Joost G J Hoenderop, Jack F M Wetzels, Dorien Lugtenberg, Tom Nijenhuis
Mice lacking distal tubular expression of CLDN10, the gene encoding the tight junction protein Claudin-10, show enhanced paracellular magnesium and calcium permeability and reduced sodium permeability in the thick ascending limb (TAL), leading to a urine concentrating defect. However, the function of renal Claudin-10 in humans remains undetermined. We identified and characterized CLDN10 mutations in two patients with a hypokalemic-alkalotic salt-losing nephropathy. The first patient was diagnosed with Bartter syndrome (BS) >30 years ago...
July 3, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28662272/campylobacter-fetus-impairs-barrier-function-in-ht-29-b6-cells-through-focal-tight-junction-alterations-and-leaks
#18
Roland Bücker, Susanne M Krug, Anja Fromm, Hans Linde Nielsen, Michael Fromm, Henrik Nielsen, Jörg-Dieter Schulzke
Infections by Campylobacter species are the most common foodborne zoonotic disease worldwide. Campylobacter jejuni and C. coli are isolated most frequently from human stool samples, but severe infections by C. fetus (Cf), which can cause gastroenteritis, septicemia, and abortion, are also found. This study aims at the characterization of pathological changes in Cf infection using an intestinal epithelial cell model. The Cf-induced epithelial barrier defects appeared earlier than those of avian Campylobacter species like C...
June 29, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28659146/antibody-targeting-of-claudin-1-as-a-potential-colorectal-cancer-therapy
#19
S Cherradi, A Ayrolles-Torro, N Vezzo-Vié, N Gueguinou, V Denis, E Combes, F Boissière, M Busson, L Canterel-Thouennon, C Mollevi, M Pugnière, F Bibeau, M Ychou, P Martineau, C Gongora, M Del Rio
BACKGROUND: Metastatic colorectal cancer (mCRC) is one of the major causes of cancer-related death. Despite the substantial progress in mCRC management, it remains important to identify new therapeutic options and biological markers for personalized medicine. Here, we investigated the expression of claudin-1 (CLDN1), a major tight junction transmembrane protein, in the different colorectal cancer (CRC) molecular subtypes and then assessed the anti-tumor effect of a new anti-CLDN1 monoclonal antibody (mAb)...
June 28, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28656265/bioinformatic-analysis-reveals-potential-properties-of-human-claudin-6-regulation-and-functions
#20
Dongjing Lin, Yaxiong Guo, Yanru Li, Yang Ruan, Mingzi Zhang, Xiangshu Jin, Minlan Yang, Yan Lu, Peiye Song, Shuai Zhao, Bing Dong, Yinping Xie, Qihua Dang, Chengshi Quan
Claudin-6 (CLDN6) is an integral component of the tight junction proteins in polarized epithelial and endothelial cells and plays a crucial role in maintaining cell integrity. Deregulation of CLDN6 expression and distribution in tumor tissues have been widely documented and correlated with cancer progression and metastasis. However, a complete mechanistic understanding of CLDN6 regulation and function remains to be studied. Herein, we show new potential properties of CLDN6 regulation and functions from bioinformatics analysis...
June 27, 2017: Oncology Reports
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