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Tight junctions claudin

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https://www.readbyqxmd.com/read/28529587/expression-of-claudin-1-4-and-7-in-thyroid-neoplasms
#1
Dinç Süren, Mustafa Yildirim, Alper Sayiner, Arsenal Sezgin Alikanoğlu, Irem Atalay, Umut Riza Gündüz, Vildan Kaya, Şeyda Gündüz, Mehmet Tahir Oruç, Cem Sezer
The distinction of thyroid carcinoma from benign thyroid neoplasm, as well as the subtyping of papillary carcinoma (PC) and follicular carcinoma (FC), may be performed histopathologically in the majority of cases. However, in certain cases, it is difficult to histopathologically distinguish between PC and FC, as well as follicular adenoma (FA), FC and the dominant nodule of multinodular goiter (MNG-DN). The present study aimed to determine the roles of the expression levels of the tight junction proteins claudin 1, 4 and 7 in the differential diagnosis of PC, FC, FA, MNG-DN, medullary carcinoma (MC) and anaplastic carcinoma (AC)...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28528309/maintenance-of-claudin-3-expression-and-the-barrier-functions-of-intercellular-junctions-in-parotid-acinar-cells-via-the-inhibition-of-src-signaling
#2
Megumi Yokoyama, Takanori Narita, Hajime Sakurai, Osamu Katsumata-Kato, Hiroshi Sugiya, Junko Fujita-Yoshigaki
OBJECTIVES: Salivary acinar and duct cells show different expression patterns of claudins, which may reflect their different functions. To study the role of claudins in saliva secretion, we examined alterations in the expression patterns of cell adhesion molecules in parotid glands of γ-irradiated rats and analyzed the influence of those changes on intercellular barrier function using primary cultures of parotid acinar cells. DESIGN: Rats were γ-irradiated with doses of 5, 15 or 20Gy, and expression levels of cell adhesion molecules were examined by immunoblotting analysis...
May 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28523565/blood-brain-barrier-permeability-is-exacerbated-in-experimental-model-of-hepatic-encephalopathy-via-mmp-9-activation-and-downregulation-of-tight-junction-proteins
#3
Saurabh Dhanda, Rajat Sandhir
The present study was designed to investigate the mechanisms involved in blood-brain barrier (BBB) permeability in bile duct ligation (BDL) model of chronic hepatic encephalopathy (HE). Four weeks after BDL surgery, a significant increase was observed in serum bilirubin levels. Masson trichrome staining revealed severe hepatic fibrosis in the BDL rats. (99m)Tc-mebrofenin retention was increased in the liver of BDL rats suggesting impaired hepatobiliary transport. An increase in permeability to sodium fluorescein, Evans blue, and fluorescein isothiocyanate (FITC)-dextran along with increase in water and electrolyte content was observed in brain regions of BDL rats suggesting disrupted BBB...
May 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28505395/claudin-peptidomimetics-modulate-tissue-barriers-for-enhanced-drug-delivery
#4
Sophie Dithmer, Christian Staat, Carolin Müller, Min-Chi Ku, Andreas Pohlmann, Thoralf Niendorf, Nora Gehne, Petra Fallier-Becker, Ágnes Kittel, Fruzsina R Walter, Szilvia Veszelka, Maria A Deli, Rosel Blasig, Reiner F Haseloff, Ingolf E Blasig, Lars Winkler
The blood-brain barrier (BBB) formed by the microvascular endothelium limits cerebral drug delivery. The paraendothelial cleft is sealed by tight junctions (TJs) with a major contribution from claudin-5, which we selected as a target to modulate BBB permeability. For this purpose, drug-enhancer peptides were designed based on the first extracellular loop (ECL) of claudin-5 to allow transient BBB permeabilization. Peptidomimetics (C5C2 and derivatives, nanomolar affinity to claudin-5) size-selectively (≤40 kDa) and reversibly (12-48 h) increased the permeability of brain endothelial and claudin-5-transfected epithelial cell monolayers...
May 15, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28504618/butyrate-reduces-high-fat-diet-induced-metabolic-alterations-hepatic-steatosis-and-pancreatic-beta-cell-and-intestinal-barrier-dysfunctions-in-prediabetic-mice
#5
V A Matheus, Lcs Monteiro, R B Oliveira, D A Maschio, C B Collares-Buzato
In this study, we investigated the effect of diet supplementation with sodium butyrate (5% w/w), a short-chain fatty acid produced by the intestinal microbiota, on metabolic parameters, body adiposity, hepatic and pancreatic lipid accumulation, beta cell function/mass as well as on the structure and function of the tight junction-mediated intestinal epithelial barrier in both normal and obese/prediabetic C57 mice fed a regular (control) or high-fat diet for 60 days, respectively. Butyrate treatment significantly inhibited all the high-fat-induced metabolic dysfunctions evaluated, i...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28501330/virus-entry-and-replication-in-the-brain-precedes-blood-brain-barrier-disruption-during-intranasal-alphavirus-infection
#6
Matthew D Cain, Hamid Salimi, Yongfeng Gong, Lihua Yang, Samantha L Hamilton, James R Heffernan, Jianghui Hou, Mark J Miller, Robyn S Klein
Viral infections of the central nervous system (CNS) are often associated with blood-brain barrier (BBB) disruption, yet the impact of virus replication and immune cell recruitment on BBB integrity are incompletely understood. Using two-photon microscopy, we demonstrate that Venezuelan equine encephalitis virus (VEEV) strain TC83-GFP, a GFP expressing, attenuated strain with a G3A mutation within the 5' UTR that is associated with increased sensitivity to type I interferons (IFNs), does not directly impact BBB permeability...
May 1, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28495656/dehydroepiandrosterone-sulfate-augments-blood-brain-barrier-and-tight-junction-protein-expression-in-brain-endothelial-cells
#7
Dimitrios Papadopoulos, Georgios Scheiner-Bobis
Tight junctions (TJ) between brain endothelial cells are essential for formation and maintenance of the blood-brain barrier (BBB). Although loss of BBB integrity is associated with several neuropathological disorders, treatments that augment or stabilise the BBB are scarce. Here we show that physiological concentrations of dehydroepiandrosterone sulfate (DHEAS) stimulate the expression of the TJ proteins zonula occludens-1 (ZO-1) and claudin-3 in the brain-derived endothelial cell line bEnd.3 and promote TJ formation between neighbouring cells, demonstrated by augmented transendothelial resistance across cell monolayers...
May 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28493961/aldosterone-signaling-regulates-the-over-expression-of-claudin-4-and-8-at-the-distal-nephron-from-type-1-diabetic-rats
#8
Eduardo Molina-Jijón, Rafael Rodríguez-Muñoz, Ricardo González-Ramírez, Carmen Namorado-Tónix, José Pedraza-Chaverri, Jose L Reyes
Hyperglycemia in diabetes alters tight junction (TJ) proteins in the kidney. We evaluated the participation of aldosterone (ALD), and the effect of spironolactone (SPL), a mineralocorticoid receptor antagonist, on the expressions of claudin-2, -4, -5 and -8, and occludin in glomeruli, proximal and distal tubules isolated from diabetic rats. Type 1 diabetes was induced in female Wistar rats by a single tail vein injection of streptozotocin (STZ), and SPL was administrated daily by gavage, from days 3-21. Twenty-one days after STZ injection the rats were sacrificed...
2017: PloS One
https://www.readbyqxmd.com/read/28493289/intestinal-epithelial-claudins-expression-and-regulation-in-homeostasis-and-inflammation
#9
REVIEW
Vicky Garcia-Hernandez, Miguel Quiros, Asma Nusrat
The intestinal epithelium forms a highly dynamic and selective barrier that controls absorption of fluid and solutes while restricting pathogen access to underlying tissues. Barrier properties are achieved by intercellular junctions that include an apical tight junction (TJ) and subjacent adherens junctions and desmosomes. The TJ tetraspan claudin proteins form pores between epithelial cells to control paracellular fluid and ion movement. In addition to regulation of barrier function, claudin family members control epithelial homeostasis and are expressed in a spatiotemporal manner in the intestinal crypt-luminal axis...
May 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28484272/effect-of-bacillus-subtilis-on-aeromonas-hydrophila-induced-intestinal-mucosal-barrier-function-damage-and-inflammation-in-grass-carp-ctenopharyngodon-idella
#10
Weiguang Kong, Can Huang, Ying Tang, Ding Zhang, Zhixin Wu, Xiaoxuan Chen
Our study explored the effect of oral intubation of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp. The mid-intestine mucosal tissue was collected for ATPase activity measurement. Intestinal mucosa was also ultrastructurally examined with transmission electron microscope (TEM), and its permeability was determined using Evans blue (EB) and D-lactic acid. The mid-intestine pro-inflammation cytokine, MyD88 and tight junction (TJ) protein mRNA expression levels were measured using real-time quantitative PCR...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28481779/claudin-4-expression-is-associated-with-survival-in-ovarian-cancer-but-not-with-chemotherapy-response
#11
Laura Martín de la Fuente, Susanne Malander, Linda Hartman, Jenny-Maria Jönsson, Anna Ebbesson, Mef Nilbert, Anna Måsbäck, Ingrid Hedenfalk
The tight junction protein claudin-4 has been reported to be overexpressed in advanced ovarian cancer. We investigated the prognostic significance of claudin-4 overexpression and whether claudin-4 expression could predict platinum response in primary ovarian carcinoma (OC). Claudin-4 expression was evaluated by immunohistochemistry in a tissue microarray of 140 OCs. Multivariable Cox-regression models were used to assess the effect of claudin-4 overexpression on progression-free survival and overall survival (OS)...
May 5, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28474805/cldn8-an-androgen-regulated-gene-promotes-prostate-cancer-cell-proliferation-and-migration
#12
Daisaku Ashikari, Kenichi Takayama, Daisuke Obinata, Satoru Takahashi, Satoshi Inoue
The proliferation of prostate cancer cells is controlled by androgen receptor (AR) signaling pathway. However, the function of AR target genes has not been fully elucidated. In the previous studies, we have identified global AR binding sites (ARBSs) and AR target genes in prostate cancer cells. Here, we focused on Claudin 8 (CLDN8), a protein constituting tight junction in cell membrane. We found one ARBS in the promoter region and two functional androgen-responsive elements (AREs) in the sequence. Reporter assay demonstrated transcriptional activation of the CLDN8 promoter by androgen is dependent on these AREs...
May 5, 2017: Cancer Science
https://www.readbyqxmd.com/read/28460142/comparative-proteomics-reveals-silver-nanoparticles-alter-fatty-acid-metabolism-and-amyloid-beta-clearance-for-neuronal-apoptosis-in-a-triple-cell-co-culture-model-of-the-blood-brain-barrier
#13
Ho-Chen Lin, Ming-Yi Ho, Chao-Ming Tsen, Chien-Chu Huang, Chin-Ching Wu, Yuh-Jeen Huang, I-Lun Hsiao, Chun-Yu Chuang
Silver nanoparticles (AgNPs) enter the central nervous system through the blood-brain barrier (BBB). AgNP exposure can increase amyloid beta (Aβ) deposition in neuronal cells to potentially induce Alzheimer disease (AD) progression. However, the mechanism through which AgNPs alter BBB permeability in endothelial cells and subsequently lead to AD progression remains unclear. This study investigated whether AgNPs disrupt the tight junction proteins of brain endothelial cells, and alter the proteomic metabolism of neuronal cells underlying AD progression in a triple cell co-culture model constructed using mouse brain endothelial (bEnd...
April 29, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28456683/patchouli-alcohol-ameliorates-dextran-sodium-sulfate-induced-experimental-colitis-and-suppresses-tryptophan-catabolism
#14
Chang Qu, Zhong-Wen Yuan, Xiu-Ting Yu, Yan-Feng Huang, Guang-Hua Yang, Jian-Nan Chen, Xiao-Ping Lai, Zi-Ren Su, Hui-Fang Zeng, Ying Xie, Xiao-Jun Zhang
Despite the increased morbidity of ulcerative colitis (UC) in recent years, available treatments remain unsatisfactory. Pogostemon cablin has been widely applied to treat a variety of gastrointestinal disorders in clinic for centuries, in which patchouli alcohol (PA, C15H26O) has been identified as the major active component. This study attempted to determine the bioactivity of PA on dextran sulfate sodium (DSS)-induced mice colitis and clarify the mechanism of action. Acute colitis was induced in mice by 3% DSS for 7 days...
April 27, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28455726/developmental-expression-of-claudins-in-the-mammary-gland
#15
Heidi K Baumgartner, Michael C Rudolph, Palaniappian Ramanathan, Valerie Burns, Patricia Webb, Benjamin G Bitler, Torsten Stein, Ken Kobayashi, Margaret C Neville
Claudins are a large family of membrane proteins whose classic function is to regulate the permeability of tight junctions in epithelia. They are tetraspanins, with four alpha-helices crossing the membrane, two extracellular loops, a short cytoplasmic N-terminus and a longer and more variable C-terminus. The extracellular ends of the helices are known to undergo side-to-side (cis) interactions that allow the formation of claudin polymers in the plane of the membrane. The extracellular loops also engage in head-to-head (trans) interactions thought to mediate the formation of tight junctions...
April 28, 2017: Journal of Mammary Gland Biology and Neoplasia
https://www.readbyqxmd.com/read/28453844/association-of-endothelial-glycocalyx-and-tight-and-adherens-junctions-with-severity-of-plasma-leakage-in-dengue-infection
#16
Suhendro Suwarto, R Tedjo Sasmono, Robert Sinto, Eppy Ibrahim, Maulana Suryamin
Background.: The role of vascular endothelial (VE) components in dengue infection with plasma leakage is unknown. Therefore, we conducted a study to determine the adjusted association of the endothelial glycocalyx layer (EGL) and tight and adherens junction markers with plasma leakage. Methods.: A prospective observational study was conducted at Cipto Mangunkusumo Hospital and Persahabatan Hospital, Jakarta, Indonesia. Adult dengue patients admitted to the hospital on the third day of fever from November 2013 through August 2015 were included in the study...
March 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28452428/impact-of-gluten-consumption-in-patients-with-functional-dyspepsia-a-case-control-study
#17
Lijun Du, Jinhua Shen, John J Kim, Huiqin He, Binrui Chen, Ning Dai
BACKGROUND AND AIM: Dietary factors and immune dysfunction may induce symptoms in patients with functional dyspepsia (FD). Aim of the study was to evaluate whether gluten consumption impacts symptom onset in patients with FD and to evaluate for possible histologic alterations in the duodenum of patients with FD. METHODS: We prospectively enrolled 101 patients newly diagnosed with FD and 31 asymptomatic controls. Specific FD symptoms and gluten consumption patterns were evaluated by self-reported questionnaires...
April 28, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28445614/two-common-human-cldn5-alleles-encode-different-open-reading-frames-but-produce-one-protein-isoform
#18
Ronald M Cornely, Barbara Schlingmann, Whitney S Shepherd, Joshua D Chandler, David C Neujahr, Michael Koval
Claudins provide tight junction barrier selectivity. The human CLDN5 gene contains a high-frequency single-nucleotide polymorphism (rs885985), where the G allele codes for glutamine (Q) and the A allele codes for an amber stop codon. Thus, these different CLDN5 alleles define nested open reading frames (ORFs) encoding claudin-5 proteins that are 303 or 218 amino acids in length. Interestingly, human claudin-16 and claudin-23 also have long ORFs. The long form of claudin-5 contrasts with the majority of claudin-5 proteins in the National Center for Biotechnology Information protein database, which are less than 220 amino acids in length...
April 26, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28445594/porcine-milk-induces-a-strengthening-of-barrier-function-in-porcine-jejunal-epithelium-in-vitro
#19
Judith Radloff, Silke S Zakrzewski, Robert Pieper, Alexander G Markov, Salah Amasheh
Milk contains a variety of components that have been shown to affect the expression and localization of epithelial tight junction proteins and therefore the intestinal barrier. Thus, we hypothesized that milk would have an effect on intestinal barrier properties, owing to effects on the tight junction in an intraspecies porcine intestinal in vitro model. Jejunal samples of piglets derived from different age groups were analyzed. Transepithelial electrical resistance was recorded employing the Ussing chamber technique...
April 26, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28438571/effect-of-intestinal-epithelial-autophagy-on-bacterial-translocation-in-severe-acute-pancreatitis
#20
Weiwei Wen, Hongmei Zheng, Yingjian Jiang, Luqiao Huang, Dehui Li, Jian Zhang, Dianliang Zhang
BACKGROUND AND OBJECTIVE: We examined the impact of autophagy activation on bacterial translocation (BT) and tight junction (TJ) proteins in the intestinal mucosa of patients with severe acute pancreatitis (SAP). METHODS: Thirty-one SAP patients were divided into two groups, BT(+) and BT(-), according to the presence of BT in the blood, as detected by 16S rDNA sequencing. Eight healthy individuals were included in the control group. Serum endotoxin levels were measured by ELISA...
April 21, 2017: Clinics and Research in Hepatology and Gastroenterology
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