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Tight junctions claudin

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https://www.readbyqxmd.com/read/28928959/cell-polarity-cell-adhesion-and-spermatogenesis-role-of-cytoskeletons
#1
REVIEW
Linxi Li, Ying Gao, Haiqi Chen, Tito Jesus, Elizabeth Tang, Nan Li, Qingquan Lian, Ren-Shan Ge, C Yan Cheng
In the rat testis, studies have shown that cell polarity, in particular spermatid polarity, to support spermatogenesis is conferred by the coordinated efforts of the Par-, Crumbs-, and Scribble-based polarity complexes in the seminiferous epithelium. Furthermore, planar cell polarity (PCP) is conferred by PCP proteins such as Van Gogh-like 2 (Vangl2) in the testis. On the other hand, cell junctions at the Sertoli cell-spermatid (steps 8-19) interface are exclusively supported by adhesion protein complexes (for example, α6β1-integrin-laminin-α3,β3,γ3 and nectin-3-afadin) at the actin-rich apical ectoplasmic specialization (ES) since the apical ES is the only anchoring device in step 8-19 spermatids...
2017: F1000Research
https://www.readbyqxmd.com/read/28928541/morphological-and-functional-analyses-of-the-tight-junction-in-the-palatal-epithelium-of-mouse
#2
Noriko Shiotsu, Tadafumi Kawamoto, Mariko Kawai, Mika Ikegame, Yasuhiro Torii, Hiroyuki Sasaki, Toshio Yamamoto
Tight junction (TJ) is one of the cell-cell junctions and known to have the barrier and fence functions between adjacent cells in both simple and stratified epithelia. We examined the distribution pattern, constitutive proteins, and permeability of TJ in the stratified squamous epithelium of the palatal mucosa of mice. Ultrastructural observations based on the ultrathin section and freeze-fracture methods revealed that poorly developed TJs are located at the upper layer of the stratum granulosum. The positive immunofluorescence of occludin (OCD), claudin (CLD)-1 and -4 were localized among the upper layer of the stratum granulosum showing a dot-like distribution pattern...
August 25, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28928120/creation-of-a-claudin-2-binder-and-its-tight-junction-modulating-activity-in-a-human-intestinal-model
#3
Mutsumi Takigawa, Manami Iida, Shotaro Nagase, Hidehiko Suzuki, Akihiro Watari, Minoru Tada, Yoshiaki Okada, Takefumi Doi, Masayoshi Fukasawa, Kiyohito Yagi, Jun Kunisawa, Masuo Kondoh
Disruption of the gastrointestinal epithelial barrier is a hallmark of chronic inflammatory bowel diseases (IBDs). The transmembrane protein claudin 2 (CLDN2) is a component of epithelial tight junctions (TJs). In the intestines of patients with IBDs, the expression of the pore-forming TJ protein CLDN2 is upregulated. Although CLDN2 is involved in these leaky barriers, whether it can be a target to enhance TJ integrity is unknown because a CLDN2-specific inhibitor has not been developed. Here, we used DNA immunization to generate a monoclonal antibody (mAb) that recognized an extracellular loop of CLDN2...
September 19, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28922365/isothiocyanate-enriched-moringa-seed-extract-alleviates-ulcerative-colitis-symptoms-in-mice
#4
Youjin Kim, Alex G Wu, Asha Jaja-Chimedza, Brittany L Graf, Carrie Waterman, Michael P Verzi, Ilya Raskin
Moringa (Moringa oleifera Lam.) seed extract (MSE) has anti-inflammatory and antioxidant activities. We investigated the effects of MSE enriched in moringa isothiocyanate-1 (MIC-1), its putative bioactive, on ulcerative colitis (UC) and its anti-inflammatory/antioxidant mechanism likely mediated through Nrf2-signaling pathway. Dextran sulfate sodium (DSS)-induced acute (n = 8/group; 3% DSS for 5 d) and chronic (n = 6/group; cyclic rotations of 2.5% DSS/water for 30 d) UC was induced in mice that were assigned to 4 experimental groups: healthy control (water/vehicle), disease control (DSS/vehicle), MSE treatment (DSS/MSE), or 5-aminosalicyic acid (5-ASA) treatment (positive control; DSS/5-ASA)...
2017: PloS One
https://www.readbyqxmd.com/read/28921512/dacomitinib-induced-diarrhea-targeting-chloride-secretion-with-crofelemer
#5
Ysabella Z A Van Sebille, Rachel J Gibson, Hannah R Wardill, Imogen A Ball, Dorothy M K Keefe, Joanne M Bowen
Dacomitinib, an irreversible small-molecule pan-ErbB TKI, has a high incidence of diarrhea which has been suggested to be due to chloride secretory mechanisms. Based on this hypothesis, crofelemer, an anti-secretory agent may be an effective intervention. T84 monolayers were treated with 1µM dacomitinib and 10µM crofelemer, and mounted into Ussing chambers for electrogenic ion analysis. Crofelemer attenuated increases in chloride secretion in cells treated with dacomitinib. Albino Wistar rats (n=48) were treated with 7...
September 16, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28912935/baicalin-attenuates-subarachnoid-hemorrhagic-brain-injury-by-modulating-blood-brain-barrier-disruption-inflammation-and-oxidative-damage-in-mice
#6
Xianqing Shi, Yongjian Fu, SongSong Zhang, Hao Ding, Jin Chen
In subarachnoid hemorrhagic brain injury, the early crucial events are edema formation due to inflammatory responses and blood-brain barrier disruption. Baicalin, a flavone glycoside, has antineuroinflammatory and antioxidant properties. We examined the effect of baicalin in subarachnoid hemorrhagic brain injury. Subarachnoid hemorrhage was induced through filament perforation and either baicalin or vehicle was administered 30 min prior to surgery. Brain tissues were collected 24 hours after surgery after evaluation of neurological scores...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28903184/dexmedetomidine-exerts-neuroprotective-effect-via-the-activation-of-the-pi3k-akt-mtor-signaling-pathway-in-rats-with-traumatic-brain-injury
#7
Min Shen, Shan Wang, Xin Wen, Xin-Rui Han, Yong-Jian Wang, Xiu-Min Zhou, Man-He Zhang, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
OBJECTIVE: This study aims to explore the neuroprotective effects of dexmedetomidine (Dex) in rats suffering from traumatic brain injury (TBI) via the PI3K/Akt/mTOR signaling pathway. METHODS: A weight drop model was performed for TBI model establishment. A total of 150 Sprague Dawley rats were selected and assigned into control, sham, TBI, TBI+Dex, TBI+LY294002 (LY) and TBI+Dex+LY groups. Modified Neurological Severity Score (mNSS) was conducted in order to evaluate the neurological injury...
September 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28901429/monocytic-cell-junction-proteins-serve-important-roles-in-atherosclerosis-via-the-endoglin-pathway
#8
Lina Chen, Zhongliang Chen, Menghua Ge, Oushan Tang, Yinhong Cheng, Haoliang Zhou, Yu Shen, Fengming Qin
The formation of atherosclerosis is recognized to be caused by multiple factors including pathogenesis in monocytes during inflammation. The current study provided evidence that monocytic junctions were significantly altered in patients with atherosclerosis, which suggested an association between cell junctions and atherosclerosis. Claudin‑1, occludin‑1 and ZO‑1 were significantly enhanced in atherosclerosis, indicating that the tight junction pathway was activated during the pathogenesis of atherosclerosis...
September 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28893958/microbial-derived-butyrate-promotes-epithelial-barrier-function-through-il-10-receptor-dependent-repression-of-claudin-2
#9
Leon Zheng, Caleb J Kelly, Kayla D Battista, Rachel Schaefer, Jordi M Lanis, Erica E Alexeev, Ruth X Wang, Joseph C Onyiah, Douglas J Kominsky, Sean P Colgan
Commensal interactions between the enteric microbiota and distal intestine play important roles in regulating human health. Short-chain fatty acids (SCFAs), such as butyrate, produced through anaerobic microbial metabolism represent a major energy source for the host colonic epithelium and enhance epithelial barrier function through unclear mechanisms. Separate studies revealed that the epithelial anti-inflammatory IL-10 receptor α subunit (IL-10RA) is also important for barrier formation. Based on these findings, we examined if SCFAs promote epithelial barrier through IL-10RA-dependent mechanisms...
September 11, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28887111/impairment-of-gill-structural-integrity-by-manganese-deficiency-or-excess-related-to-induction-of-oxidative-damage-apoptosis-and-dysfunction-of-the-physical-barrier-as-regulated-by-nf-%C3%AE%C2%BAb-caspase-and-nrf2-signaling-in-fish
#10
Wei-Dan Jiang, Ren-Jun Tang, Yang Liu, Pei Wu, Sheng-Yao Kuang, Jun Jiang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou, Lin Feng
This study is for the first time to explore the possible effects of dietary manganese (Mn) on structural integrity and the related signaling in the gills of fish. Grass carp (Ctenopharyngodon idella) were fed with six diets containing graded levels of Mn [3.65-27.86 mg Mn/kg diet] for 8 weeks. The results firstly demonstrated that Mn deficiency aggravated inflammation indicated by up-regulation of pro-inflammatory cytokines (tumour necrosis factor α, interleukin 8, and interleukin 1β mRNA levels) and down-regulation of anti-inflammatory cytokines (interleukin 10, transforming growth factor-β1) mRNA levels, which might be partially related to the up-regulation of nuclear factor kappa B (NF-κB p65) and down-regulation of nuclear inhibitor factor κBα (iκBα) mRNA levels in the gills of fish...
September 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28883651/the-role-of-transcriptional-factor-p63-in-regulation-of-epithelial-barrier-and-ciliogenesis-of-human-nasal-epithelial-cells
#11
Yakuto Kaneko, Takayuki Kohno, Takuya Kakuki, Ken-Ichi Takano, Noriko Ogasawara, Ryo Miyata, Shin Kikuchi, Takumi Konno, Tsuyoshi Ohkuni, Ryoto Yajima, Akito Kakiuchi, Shin-Ichi Yokota, Tetsuo Himi, Takashi Kojima
Disruption of nasal epithelial tight junctions (TJs) and ciliary dysfunction are found in patients with chronic rhinosinusitis (CRS) and nasal polyps (NPs), along with an increase of p63-positive basal cells and histone deacetylase (HDAC) activity. To investigate these mechanisms, primary cultures of HNECs transfected with human telomerase reverse transcriptase (hTERT-HNECs) were transfected with siRNAs of TAp63 and ΔNp63, treated with the NF-kB inhibitor curucumin and inhibitors of HDACs, and infected with respiratory syncytial virus (RSV)...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28882647/acute-and-chronic-exposure-to-high-levels-of-glucose-modulates-tight-junction-associated-epithelial-barrier-function-in-a-renal-tubular-cell-line
#12
B M Mongelli-Sabino, L P Canuto, C B Collares-Buzato
AIMS: Type 2 diabetes mellitus (T2DM) is one of the most prevalent diseases worldwide. Diabetic nephropathy (DN) is a complication of diabetes and the mechanisms underlying onset and progression of this disease are not fully understood. It has been shown that hyperglycemia is an independent factor to predict the development of DN in individuals with T2DM, however, a link between high plasma glucose levels and renal tubular injuries in DN remains unknown. In this study, we investigated the effect of high levels of glucose (i...
September 4, 2017: Life Sciences
https://www.readbyqxmd.com/read/28878076/zika-virus-infects-human-sertoli-cells-and-modulates-the-integrity-of-the-in-vitro-blood-testis-barrier-model
#13
David N Siemann, Daniel P Strange, Payal N Maharaj, Pei-Yong Shi, Saguna Verma
Confirmed reports of ZIKV in human seminal fluid for months after the clearance of viremia suggest the ability of ZIKV to establish persistent infection in the seminiferous tubules, an immune privileged site in the testis protected by the blood-testis barrier, also called the Sertoli cell barrier (SCB). However, cellular targets of ZIKV in human testis and mechanisms by which the virus enters seminiferous tubules remain unclear. We demonstrate that primary human SCs were highly susceptible to ZIKV as compared to the closely related dengue virus and induced expression of IFN-α, key cytokines and cell-adhesion molecules (VCAM-1 and ICAM-1)...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28869648/site-specific-distribution-of-claudin-based-paracellular-channels-with-roles-in-biological-fluid-flow-and-metabolism
#14
REVIEW
Hiroo Tanaka, Atsushi Tamura, Koya Suzuki, Sachiko Tsukita
The claudins are a family of membrane proteins with at least 27 members in humans and mice. The extracellular regions of claudin proteins play essential roles in cell-cell adhesion and the paracellular barrier functions of tight junctions (TJs) in epithelial cell sheets. Furthermore, the extracellular regions of some claudins function as paracellular channels in the paracellular barrier that allow the selective passage of water, ions, and/or small organic solutes across the TJ in the extracellular space. Structural analyses have revealed a common framework of transmembrane, cytoplasmic, and extracellular regions among the claudin-based paracellular barriers and paracellular channels; however, differences in the claudins' extracellular regions, such as their charges and conformations, determine their properties...
September 4, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28864200/vascular-expression-of-angiopoietin1-%C3%AE-5%C3%AE-1-integrin-and-tight-junction-proteins-is-tightly-regulated-during-vascular-remodeling-in-the-post-ischemic-brain
#15
Jialan Sun, Liming Yu, Shu Huang, Xiaoyin Lai, Richard Milner, Longxuan Li
The post-stroke angiogenic response is accompanied by changes of tight junctions (TJs) of the blood-brain barrier (BBB). However, the precise dynamic change of TJ proteins (TJPs) in the different stages of stroke-induced vascular remodeling and the molecules mediating these processes have yet to be fully defined. To investigate the temporal relationship between changes in TJPs, the pro-angiogenic factor α5β1 integrin and the anti-permeability factor Ang1 in cerebral vessels following cerebral ischemic stroke, male C57Bl/6 mice were subject to 90min of ischemia by temporary occlusion of the middle cerebral artery followed by reperfusion and their brains analyzed 0, 1, 2, 4, 7 and 14days post-ischemia...
August 31, 2017: Neuroscience
https://www.readbyqxmd.com/read/28863193/a-refined-model-of-claudin-15-tight-junction-paracellular-architecture-by-molecular-dynamics-simulations
#16
Giulio Alberini, Fabio Benfenati, Luca Maragliano
Tight-junctions between epithelial cells of biological barriers are specialized molecular structures that regulate the flux of solutes across the barrier, parallel to cell walls. The tight-junction backbone is made of strands of transmembrane proteins from the claudin family, but the molecular mechanism of its function is still not completely understood. Recently, the crystal structure of a mammalian claudin-15 was reported, displaying for the first time the detailed features of transmembrane and extracellular domains...
2017: PloS One
https://www.readbyqxmd.com/read/28861400/entamoeba-histolytica-ehcp112-dislocates-and-degrades-claudin-1-and-claudin-2-at-tight-junctions-of-the-intestinal-epithelium
#17
Patricia Cuellar, Elizabeth Hernández-Nava, Guillermina García-Rivera, Bibiana Chávez-Munguía, Michael Schnoor, Abigail Betanzos, Esther Orozco
During intestinal invasion, Entamoeba histolytica opens tight junctions (TJs) reflected by transepithelial electrical resistance (TEER) dropping. To explore the molecular mechanisms underlying this, we studied in vitro and in vivo the damage produced by the recombinant E. histolytica cysteine protease (rEhCP112) on TJ functions and proteins. rEhCP112 reduced TEER in Caco-2 cells in a dose- and time-dependent manner; and EhCP112-overexpressing trophozoites provoked major epithelial injury compared to control trophozoites...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28859979/bortezomib-a-proteasome-inhibitor-alleviates-atopic-dermatitis-by-increasing-claudin-1-protein-expression
#18
Yong-Eun Kim, Namjoon Cho, Seonghye Cheon, Kee K Kim
Atopic dermatitis (AD) is a chronic inflammatory skin disease. Many studies investigating AD pathogenesis and its therapy have been conducted but none have been successful. One of the causes of AD is dysfunction of tight junctions through reduction of claudin 1 expression in the epidermal barrier of the skin. In the present study, we investigated the role of bortezomib (BTZ) in the restoration of the reduced expression of claudin 1. Immunoblot and immunofluorescence analyses revealed that BTZ increased the protein expression level of claudin 1 in the human keratinocyte cell line HaCaT, thereby forming paracellular barriers...
August 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28856667/respiratory-syncytial-virus-infection-influences-tight-junction-integrity
#19
Jeannette I Kast, Amanda J McFarlane, Anna Głobińska, Milena Sokolowska, Paulina Wawrzyniak, Marek Sanak, Jürgen Schwarze, Cezmi A Akdis, Kerstin Wanke
Background Respiratory syncytial virus (RSV) is an important risk factor of asthma development and is responsible for severe respiratory tract infections. However, the influence of RSV infection on barrier function of bronchial epithelial cells in vitro and in vivo is still unclear. Objective The aim of this study was to analyze the role of RSV in tight junction (TJ) regulation and to compare epithelial integrity between asthmatic and healthy individuals upon RSV infection. Methods Healthy and asthmatic human bronchial epithelial cells (HBECs) were differentiated at air-liquid interface (ALI) and infected with RSV and UV-irradiated RSV...
August 30, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28855873/molecular-characterization-of-barrier-properties-in-follicle-associated-epithelium-of-porcine-peyer-s-patches-reveals-major-sealing-function-of-claudin-4
#20
Judith Radloff, Evgeny L Falchuk, Alexander G Markov, Salah Amasheh
The pig represents a preferred model for the analysis of intestinal immunology. However, the barrier of the follicle-associated epithelium (FAE) covering porcine Peyer's patches (PP) has not yet been characterized in detail. This study aimed to perform this characterization in order to pave the way toward an understanding of the functional contribution of epithelial barrier properties in gut immunology. Porcine tissue specimens were taken from the distal small intestine in order to obtain electrophysiological data of PP FAE and neighboring villous epithelium (VE), employing the Ussing chamber technique...
2017: Frontiers in Physiology
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