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Claudin-1

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https://www.readbyqxmd.com/read/28339048/agr2-ameliorates-tumor-necrosis-factor-%C3%AE-induced-epithelial-barrier-dysfunction-via-suppression-of-nf-%C3%AE%C2%BAb-p65-mediated-mlck-p-mlc-pathway-activation
#1
Xiaolin Ye, Mei Sun
Intestinal epithelial barrier dysfunction plays a critical role in the pathogenesis of inflammatory bowel disease (IBD). Anterior gradient protein 2 homologue (AGR2) assists in maintaining intestinal homeostasis in dextran sulphate sodium-induced mouse ileocolitis; however, it is unclear whether it modulates intestinal barrier function. Our study aimed to investigate the protective role of AGR2 in tumor necrosis factor (TNF)-α-induced intestinal epithelial barrier injury. Caco-2 cell monolayers were pre-transfected with an AGR2 plasmid and then exposed to TNF-α...
March 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28335773/oral-administration-of-propionic-acid-during-lactation-enhances-the-colonic-barrier-function
#2
Zhaobo Xia, Yijiang Han, Ke Wang, Shikun Guo, Dazhou Wu, Xiaozhong Huang, Zhongrong Li, Libin Zhu
BACKGROUND: Propionic acid is a three-carbon short chain fatty acid (SCFA) that has various effects on colonic functions. Although several studies have shown the effects of propionic acid on intestinal mucosal barrier function, studies of the promotion effect during pre-weaning are rare in the literature as far as we know. METHODS: Pre-weaning male Sprague-Dawley rats 7 days after birth were given an oral 0.2 mL/10 g of 200 mM propionic acid solution in the propionic acid group or normal saline solution in the control group by gavage twice a day for ten days...
March 23, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28329012/tracking-the-stochastic-fate-of-cells-of-the-renin-lineage-after-podocyte-depletion-using-multicolor-reporters-and-intravital-imaging
#3
Natalya V Kaverina, Hiroyuki Kadoya, Diana G Eng, Michael E Rusiniak, Maria Luisa S Sequeira-Lopez, R Ariel Gomez, Jeffrey W Pippin, Kenneth W Gross, Janos Peti-Peterdi, Stuart J Shankland
Podocyte depletion plays a major role in focal segmental glomerular sclerosis (FSGS). Because cells of the renin lineage (CoRL) serve as adult podocyte and parietal epithelial cell (PEC) progenitor candidates, we generated Ren1cCre/R26R-ConfettiTG/WT and Ren1dCre/R26R-ConfettiTG/WT mice to determine CoRL clonality during podocyte replacement. Four CoRL reporters (GFP, YFP, RFP, CFP) were restricted to cells in the juxtaglomerular compartment (JGC) at baseline. Following abrupt podocyte depletion in experimental FSGS, all four CoRL reporters were detected in a subset of glomeruli at day 28, where they co-expressed de novo four podocyte proteins (podocin, nephrin, WT-1 and p57) and two glomerular parietal epithelial cell (PEC) proteins (claudin-1, PAX8)...
2017: PloS One
https://www.readbyqxmd.com/read/28327343/astilbin-from-engelhardtia-chrysolepis-enhances-intestinal-barrier-functions-in-caco-2-cell-monolayers
#4
Tatsuo Nakahara, Yosuke Nishitani, Shin Nishiumi, Masaru Yoshida, Takeshi Azuma
Astilbin, which is one of polyphenolic compounds isolated from the leaves of Engelhardtia chrysolepis HANCE (Chinese name, huang-qui), is available as the effective component in food and cosmetics because of its anti-oxidant and anti-inflammatory effects. The tight junction (TJ) proteins, which protect the body from foreign substances, are related to adhesion between a cell and a cell. Previously, the enhancement of TJ's functions induced by aglycones of flavonoids has been demonstrated, but the effects of the glycosides such as astilbin have not been observed yet...
March 18, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28325366/whorling-cellular-perineurioma-a-previously-undescribed-variant-closely-mimicking-monophasic-fibrous-synovial-sarcoma
#5
Michael Michal, Dmitry V Kazakov, Abbas Agaimy, Marta Hosova, Kvetoslava Michalova, Petr Grossmann, Petr Steiner, Faruk Skenderi, Semir Vranic, Michal Michal
The authors present a distinctive perineurioma (PN) variant which morphologically strongly resembles monophasic fibrous synovial sarcoma (MSS). The patients were 3 males and 1 female. The age ranged from 15 to 61years (mean: 44years). Locations included the sole, lower jaw, palm and foot. The tumor size ranged from 1.3cm to 2.5cm in the largest dimension (mean 1.8cm). Morphologically, all tumors had an identical, monotonous appearance. The perineurial cells were closely packed and created a confluent cellular whorls and/or sheets in a scarce stroma, with only focally discernible long, slender cytoplasmic processes typical for perineurial differentiation...
April 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28323272/constipation-predominant-irritable-bowel-syndrome-females-have-normal-colonic-barrier-and-secretory-function
#6
Stephanie A Peters, Shoko Edogawa, Wendy J Sundt, Roy B Dyer, Daniel A Dalenberg, Amelia Mazzone, Ravinder J Singh, Natalie Moses, Thomas C Smyrk, Christopher Weber, David R Linden, Wallace K MacNaughton, Jerrold R Turner, Michael Camilleri, David A Katzka, Gianrico Farrugia, Madhusudan Grover
OBJECTIVES: The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function. METHODS: A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g (13)C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression...
March 21, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28322937/snail-induced-epithelial-to-mesenchymal-transition-enhances-p-gp-mediated-multi-drug-resistance-in-hcc827-cells
#7
Takumi Tomono, Kentaro Yano, Takuo Ogihara
Overexpression and/or activation of P-glycoprotein (P-gp), which mediates efflux transport of various anti-cancer drugs in cancer cells, are associated with multi-drug resistance (MDR). On the other hand, malignant cancer cells frequently undergo epithelial-to-mesenchymal transition (EMT), thereby acquiring high migratory mobility and invasive ability. Snail is a transcriptional factor that represses multiple other factors, and its overexpression is a trigger of EMT. Since both P-gp and Snail are involved in malignant evolution of cancer, in this work, we evaluated whether or not EMT induced by overexpression of Snail influences P-gp expression and/or activity...
March 17, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28316062/cldn-1-promoted-the-epithelial-to-migration-and-mesenchymal-transition-emt-in-human-bronchial-epithelial-cells-via-notch-pathway
#8
Jing Lv, Baohua Sun, Zhitao Mai, Mingming Jiang, Junfeng Du
Claudin-1 (CLDN-1) is one of main tight junction components that play an important role in epithelial-mesenchymal transition (EMT). However, the effects of CLDN-1 on the migration and EMT induced by TGF-β1 in primary normal human bronchial epithelial (NHBE) and BEAS-2B cells have not been clear. The expression of CLDN-1 was quantified by Western blotting in NHBE and BEAS-2B cells. Cell migration and invasion were detected using transwell assays. The expression level of E-cadherin, N-cadherin, α-SMA, and Vimentin was evaluated by quantitative real-time PCR and Western blotting...
March 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28299836/involvement-of-pka-signalling-in-anti-inflammatory-effects-of-chitosan-oligosaccharides-in-ipec-j2-porcine-epithelial-cells
#9
J W Yang, G Tian, D W Chen, Y Yao, J He, P Zheng, X B Mao, J Yu, Z Q Huang, B Yu
Weaning is characterized by intestinal inflammation, which is a big challenge in pig industry. Control of intestinal inflammation is important for improvement of growth performance and health. Therefore, the study was focused on the anti-inflammatory activity of low-molecular-weight chitosan oligosaccharide (LCOS) in a porcine small intestinal epithelial cell line (IPEC-J2). The results showed that TNF-α, as inflammation inducer, significantly upregulated the mRNA expression of IL-8 and MCP-1. Afterwards, LCOS significantly attenuated mRNA expression of IL-8 and MCP-1 induced by TNF-α in the cells...
March 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28295005/-the-expression-of-claudins-in-colonic-neoplasms
#10
N A Oleinikova, O A Kharlova, P G Mal'kov, N V Danilova
AIM: the evaluation of localization claudin-1, -3 and -4 types of cancer and colon polyps. MATERIAL AND METHODS: The study included 32 colon adenocarcinoma and 86 polyps. Antibody claudin-1; -3 and -4 were used as immunohistochemical markers in this study. RESULTS: 84/118, 64/118, 52/118 reaction with claudin-1, claudin-3 and claudin-4 in cancer and colon polyps had a membrane localization, respectively. In 33 (27.9%) cases was found paradoxical reaction claudin-1; in 50 (42...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/28292027/claudin-1-regulates-pulmonary-artery-smooth-muscle-cell-proliferation-through-the-activation-of-erk1-2
#11
Xiandong Cheng, Yi Wang, Huilong Chen, Yongjian Xu, Weining Xiong, Tao Wang
Tumor necrosis factor alpha (TNF-α), a crucial inflammatory cytokine, is involved in the pathogenesis of pulmonary arterial hypertension (PAH). TNF-α can induce claudin-1 (CLDN1) expression and CLDN1 has been reported to be associated with the regulation of cellular functions including cell proliferation, migration. Thus, we aimed to explore whether CLDN1 participated in the etiology of PAH. Our study showed that CLDN1 expression was markedly increased in the lungs of rats with monocrotaline(MCT)-induced PAH, especially in the pulmonary arterial smooth muscle sections...
March 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28273404/association-of-genetic-polymorphisms-of-claudin-1-with-small-vessel-vascular-dementia
#12
Vivek Srinivasan, Nady Braidy, Ying Hua Xu, Peter Xie, Kiran Kancherla, Sashiruben Chandramohan, Eunice Kar Wing Chan, Daniel Ky Chan
The most recent hypothesis of the development of small vessel vascular dementia (VaD) emphasises the role of blood-brain barrier (BBB) dysfunction. It is hypothesised that certain genetic polymorphisms of the BBB tight junction claudin-1 protein, in combination with adverse environmental risk factors, increase the risk of BBB dysfunction and small vessel VaD. In this case-control study, 97 control participants, with a mean Mini Mental State Exam (MMSE) score of 29.1, and 38 VaD participants were recruited and completed a questionnaire on their medical history and lifestyle factors...
March 8, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28260013/wogonin-protects-human-retinal-pigment-epithelium-cells-from-lps-induced-barrier-dysfunction-and-inflammatory-responses-by-regulating-the-tlr4-nf-%C3%AE%C2%BAb-signaling-pathway
#13
Chen Chen, Danni Guo, Guohua Lu
Inflammation in the retinal pigment epithelium is an important contributor to the pathogenesis of age-related macular degeneration. Wogonin is a flavonoid isolated from the root of Scutellaria baicalensis and has multiple pharmacological effects, including anti‑inflammatory effects. The present study sought to determine if the pharmacological effects of wogonin were relevant to the treatment of AMD. ARPE‑19 cells were pre‑conditioned with different concentrations of wogonin (0‑50 µM) prior to induction of inflammation with LPS (2 µg/ml)...
February 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28259959/overexpression-of-tmprss4-promotes-tumor-proliferation-and-aggressiveness-in-breast-cancer
#14
Xiao-Mei Li, Wen-Lou Liu, Xu Chen, Ya-Wen Wang, Duan-Bo Shi, Hui Zhang, Ran-Ran Ma, Hai-Ting Liu, Xiang-Yu Guo, Feng Hou, Ming Li, Peng Gao
Transmembrane protease serine 4 (TMPRSS4) is a novel type II transmembrane serine protease that is overexpressed in various types of human cancers and has an important function in cancer progression. However, there is a paucity of data available regarding the biological effects of TMPRSS4 on breast cancer (BC) cells and the underlying mechanisms. In this study, expression of TMPRSS4 in BC tissues was detected by immunohistochemistry. The relationship between TMPRSS4 expression and clinicopathological characteristics as well as prognosis was evaluated...
February 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28259823/evaluation-of-permeability-alteration-and-epithelial-mesenchymal-transition-induced-by-transforming-growth-factor-%C3%AE-1-in-a549-nci-h441-and-calu-3-cells-development-of-an-in-vitro-model-of-respiratory-epithelial-cells-in-idiopathic-pulmonary-fibrosis
#15
Kohei Togami, Kotaro Yamaguchi, Sumio Chono, Hitoshi Tada
INTRODUCTION: Idiopathic pulmonary fibrosis (IPF) is a lethal lung disease, which is accompanied by changes in lung structure. With regard to treatment, aerosolized drugs administered intrapulmonarily are rapidly distributed into the plasma and do not remain in the lungs due to damage to the alveolar epithelium that occurs from pulmonary fibrosis. In this study, we sought to develop an in vitro model of respiratory epithelial cells in IPF for the evaluation of the intrapulmonary distribution of aerosolized drugs...
March 1, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/28259136/therapeutic-effects-of-different-doses-of-polyethylene-glycosylated-porcine-glucagon-like-peptide-2-on-ulcerative-colitis-in-male-rats
#16
Ke-Ke Qi, Jia-Jia Lv, Jie Wu, Zi-Wei Xu
BACKGROUND: Polyethylene glycosylated (PEGylated) porcine glucagon-like peptide-2 (pGLP-2) considerably increases half-life and stability compared with the native pGLP-2, but the effective dose for intestinal damage is still unclear. This study aims to evaluate the available dose of polyethylene glycosylated porcine glucagon-like peptide-2 (PEG-pGLP-2), a modified, long-acting form of pGLP-2 in an experimental rat model of ulcerative colitis. METHODS: Thirty-five male rats were randomly assigned into five groups: control, dextran sodium sulphate (DSS), DSS + PEG-pGLP-2(L), DSS + PEG-pGLP-2(M) and DSS + PEG-pGLP-2(H)...
March 4, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28246473/effect-of-toll-like-receptor-3-agonist-poly-i-c-on-intestinal-mucosa-and-epithelial-barrier-function-in-mouse-models-of-acute-colitis
#17
Hong-Wei Zhao, Yue-Hong Yue, Hua Han, Xiu-Li Chen, Yong-Gang Lu, Ji-Min Zheng, Hong-Tao Hou, Xiao-Meng Lang, Li-Li He, Qi-Lu Hu, Zi-Qian Dun
AIM: To investigate potential effects of poly I:C on mucosal injury and epithelial barrier disruption in dextran sulfate sodium (DSS)-induced acute colitis. METHODS: Thirty C57BL/6 mice were given either regular drinking water (control group) or 2% (w/v) DSS drinking water (model and poly I:C groups) ad libitum for 7 d. Poly I:C was administrated subcutaneously (20 μg/mouse) 2 h prior to DSS induction in mice of the poly I:C group. Severity of colitis was evaluated by disease activity index, body weight, colon length, histology and myeloperoxidase (MPO) activity, as well as the production of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin 17 (IL-17) and interferon-γ (IFN-γ)...
February 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28245149/airborne-particulate-matter-induces-non-allergic-eosinophilic-sinonasal-inflammation-in-mice
#18
Murugappan Ramanathan, Nyall R London, Anuj Tharakan, Nitya Surya, Thomas E Sussan, Xiaoquan Rao, Sandra Y Lin, Elina Toskala, Sanjay Rajagopalan, Shyam Biswal
RATIONALE: Exposure to airborne particulate matter has been linked to aggravation of respiratory symptoms, increased risk of cardiovascular disease, and all-cause mortality. While the health effects of particulate matter on the lower pulmonary airway have been extensively studied, little is known regarding the impact of chronic particulate matter exposure on the upper sinonasal airway. OBJECTIVES: To test the impact of chronic airborne particulate matter exposure on the upper respiratory system in vivo...
February 28, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28244853/upregulation-of-cd147-promotes-metastasis-of-cholangiocarcinoma-by-modulating-the-epithelial-to-mesenchymal-transitional-process
#19
Paweena Dana, Ryusho Kariya, Kulthida Vaeteewoottacharn, Kanlayanee Sawanyawisuth, Wunchana Seubwai, Kouki Matsuda, Seiji Okada, Sopit Wongkham
CD147 is a trans-membrane protein that can induce the expression and activity of matrix metalloproteinases (MMP). Expression of CD147 has been shown to potentiate cell migration, invasion, and metastasis of cancer. In this study, the critical role of CD147 in metastasis was elucidated using CD147-overexpressing cholangiocarcinoma (CCA) cells, <em>in vitro</em> and <em>in vivo</em>. The demonstrated molecular mechanism herein, supported that metastasis increased in CD147-overexpressing cells...
December 15, 2016: Oncology Research
https://www.readbyqxmd.com/read/28230606/secretions-of-bifidobacterium-infantis-and-lactobacillus-acidophilus-protect-intestinal-epithelial-barrier-function
#20
Shuangshuang Guo, Tyler Gillingham, Yuming Guo, Di Meng, Weishu Zhu, W Allen Walker, Kriston Ganguli
OBJECTIVES: The secreted metabolites of probiotics are cytoprotective to intestinal epithelium and have been shown to attenuate inflammation and reduce gut permeability. The present study was designed to determine the protective effects of probiotic conditioned media (PCM) from Bifidobacterium infantis (BCM) and Lactobacillus acidophilus (LCM) on interleukin (IL)-1β-induced intestinal barrier compromise. METHODS: The epithelial barrier was determined by measuring the transepithelial electrical resistance (TER) across a Caco-2 cell monolayer using a Transwell model...
March 2017: Journal of Pediatric Gastroenterology and Nutrition
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