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Claudin-1

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https://www.readbyqxmd.com/read/29129493/overexpression-of-microrna-495-improves-the-intestinal-mucosal-barrier-function-by-targeting-stat3-via-inhibition-of-the-jak-stat3-signaling-pathway-in-a-mouse-model-of-ulcerative-colitis
#1
Xian-Qun Chu, Jing Wang, Guang-Xiang Chen, Guan-Qi Zhang, De-Yong Zhang, Yong-Yan Cai
We aim to investigate the role of microRNA-495 (miR-495) in the intestinal mucosal barrier by indirectly targeting signal transducer and activator of transcription 3 (STAT3) through the Janus kinase-signal transducer and activator of transcription (JAK)/STAT3 signaling pathway in a mouse model of ulcerative colitis (UC). BALB/c mice were selected for establishing mice model of UC, and intestinal tissues of normal and UC mice were collected. ELISA was conducted for detecting levels of TNF-α, IL-6, IFN-γ and IL-10...
October 12, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29123511/a-high-grain-diet-dynamically-shifted-the-composition-of-mucosa-associated-microbiota-and-induced-mucosal-injuries-in-the-colon-of-sheep
#2
Yue Wang, Lei Xu, Junhua Liu, Weiyun Zhu, Shengyong Mao
This study investigated the dynamic shifts in mucosa-associated microbiota composition and mucosal morphology in the colon of sheep fed a high grain (HG) diet. A total of 20 male sheep were randomly assigned to four groups (n = 5 for each). The sheep in first group received hay diet. The animals in other 3 groups were fed an HG diet for 7 (HG7), 14 (HG14), or 28 (HG28) days, respectively. Colonic digesta samples were collected to determine the pH and the concentrations of volatile fatty acid (VFA) and lactate...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29116027/poly-ethylene-glycol-modified-silk-fibroin-membrane-as-a-carrier-for-limbal-epithelial-stem-cell-transplantation-in-a-rabbit-lscd-model
#3
Yijian Li, Yuli Yang, Lei Yang, Yuxiao Zeng, Xiaowei Gao, Haiwei Xu
BACKGROUND: Limbal epithelial stem cells (LESCs) play important roles in corneal epithelial homeostasis and regeneration, and damage to the limbus will lead to limbal stem cell deficiency (LSCD), with conjunctivalization and even visual impairment. Cultured LESCs have been used for ocular surface reconstruction, and silk fibroin (SF) membranes have shown potential as a substrate for LESC cultivation. Both culture methods and the carriers of LESCs affect outcomes following LESC transplantation...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29097871/fecal-microbiota-transplantation-prevents-hepatic-encephalopathy-in-rats-with-carbon-tetrachloride-induced-acute-hepatic-dysfunction
#4
Wei-Wei Wang, Yu Zhang, Xiao-Bing Huang, Nan You, Lu Zheng, Jing Li
AIM: To investigate whether fecal microbiota transplantation (FMT) prevents hepatic encephalopathy (HE) in rats with carbon tetrachloride (CCl4)-induced acute hepatic dysfunction. METHODS: A rat model of HE was established with CCl4. Rat behaviors and spatial learning capability were observed, and hepatic necrosis, intestinal mucosal barrier, serum ammonia levels and intestinal permeability were determined in HE rats receiving FMT treatment. Furthermore, the expression of tight junction proteins (Claudin-1, Claudin-6 and Occludin), Toll-like receptor (TLR) 4/TLR9, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α was examined...
October 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29075202/mitochondrial-atp-depletion-disrupts-caco-2-monolayer-integrity-and-internalizes-claudin-7
#5
Lonneke M JanssenDuijghuijsen, Sander Grefte, Vincent C J de Boer, Lara Zeper, Dorien A M van Dartel, Inge van der Stelt, Melissa Bekkenkamp-Grovenstein, Klaske van Norren, Harry J Wichers, Jaap Keijer
Objective:In vivo studies suggest that intestinal barrier integrity is dependent on mitochondrial ATP production. Here, we aim to provide mechanistic support, using an in vitro model mimicking the oxidative in vivo situation. Methods: Human Caco-2 cells were cultured for 10 days in culture flasks or for 14 days on transwell inserts in either glucose-containing or galactose-containing medium. Mitochondria were visualized and cellular respiration and levels of oxidative phosphorylation (OXPHOS) proteins were determined...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29069891/additive-effects-of-rebamipide-plus-proton-pump-inhibitors-on-the-expression-of-tight-junction-proteins-in-a-rat-model-of-gastro-esophageal-reflux-disease
#6
Tae-Geun Gweon, Jong-Hyung Park, Byung-Wook Kim, Yang Kyu Choi, Joon Sung Kim, Sung Min Park, Chang Whan Kim, Hyung-Gil Kim, Jun-Won Chung
Background/Aims: The aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD). Methods: GERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups...
October 27, 2017: Gut and Liver
https://www.readbyqxmd.com/read/29057334/antouka-super-%C3%A2-induced-oxidative-stress-and-reproductive-toxicity-in-male-japanese-quail-coturnix-coturnix-japonica
#7
Ngoula Ferdinand, Ngoumtsop Victor Herman, Ngouateu Kenfack Omer Bebe, Kenfack Augustave, Mutwedu Valence, Nguemmogne Tamdem Ghislaine, Tchoffo Herve, Azafack Kana Dorice, Deutcheu Sorelle, Manjeli Yacouba
BACKGROUND: Antouka Super(®) (AS), a combination of insecticide (Pirimiphos-methyl 16% and Permethrin 3%), is one of the most widely used pesticides in agriculture, public health, home and garden, with high potential for human and animal exposure. OBJECTIVE: The present study was undertaken to evaluate the effect of AS on the serum testosterone, oxidative stress biomarkers, testis histology and fertility of male Japanese quail. METHODS: Thirty-two (32) male Japanese quails twenty-eight (28) days were randomly divided into four groups: C0 (control), T1, T2 and T3, exposed daily (gavage) to 0, 37...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29053872/resveratrol-alleviates-heat-stress-induced-impairment-of-intestinal-morphology-microflora-and-barrier-integrity-in-broilers
#8
C Zhang, X H Zhao, L Yang, X Y Chen, R S Jiang, S H Jin, Z Y Geng
This study was to investigate the effect of resveratrol on intestinal morphology, microfloras, and barrier integrity of broilers subjected to heat stress. Two-hundred-seventy 21-day-old Cobb male broilers were randomly allocated to 3 treatment groups, each of which included 6 replicates with 15 birds per replicate. The 3 treatment groups were as follows: the control group, in which birds were exposed to thermoneutral condition (22 ± 1°C), and the heat stress group and heat stress + resveratrol (400 mg/kg) group, in which birds were exposed to cyclic heat stress (33 ± 1°C for 10 h/d from 0800 to 1800 h and 22 ± 1°C for the remaining time...
October 10, 2017: Poultry Science
https://www.readbyqxmd.com/read/29039479/mdig-suppresses-epithelial-mesenchymal-transition-and-inhibits-the-invasion-and-metastasis-of-non%C3%A2-small-cell-lung-cancer-via-regulating-gsk-3%C3%AE-%C3%AE-catenin-signaling
#9
Feng Geng, Zhanshi Jiang, Xiaogang Song, Haomin Zhou, Hongwen Zhao
Mineral dust-induced gene (mdig) can inhibit the invasion and metastasis of A549 cells. The main purpose of this study was to explore the molecular mechanism underlying the inhibitory effect of mdig on cell invasion and metastasis. Mdig-knockdown and mdig-overexpressing A549 cells and an mdig-overexpressing human umbilical vein endothelial cell (HUVEC) line were constructed using lentiviral vectors, and western blot analysis was performed to verify the silencing and overexpression of the mdig protein. A Transwell invasion assay was used to detect the invasive abilities of each experimental group, and Transwell migration and scratch assays were used to detect cell migration ability...
October 12, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29034530/gene-expression-and-morphological-changes-in-the-intestinal-mucosa-associated-with-increased-permeability-induced-by-short-term-fasting-in-chickens
#10
S Gilani, G S Howarth, G Nattrass, S M Kitessa, R Barekatain, R E A Forder, C D Tran, R J Hughes
Short-term fasting for 4.5 and 9 hr has been demonstrated to increase intestinal permeability (IP) in chickens. This study aimed to investigate the effects of 0, 4.5, 9 and 19.5 hr fasting on intestinal gene expression and villus-crypt architecture of enterocytes in jejunal and ileal samples. On day 38, Ross-308 male birds were fasted according to their group and then euthanised. Two separate intestinal sections (each 2 cm long, jejunum and ileum) were collected. One section was utilised for villus height and crypt depth measurements...
October 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29032661/a-prospective-study-of-eosinophilic-esophagitis-and-the-expression-of-tight-junction-proteins-in-patients-with-gastroesophageal-reflux-disease-symptoms
#11
Kenichiro Okimoto, Makoto Arai, Hideaki Ishigami, Keiko Saito, Shoko Minemura, Daisuke Maruoka, Tomoaki Matsumura, Tomoo Nakagawa, Tatsuro Katsuno, Masaki Suzuki, Yukio Nakatani, Osamu Yokosuka
Background/Aims: Eosinophilic esophagitis (EoE) is often erroneously diagnosed as gastroesophageal reflex disease (GERD). The aim of this study is to investigate the prevalence of EoE and the expression of tight junction (TJ) proteins in patients with GERD symptoms. Methods: One hundred patients with GERD symptoms and 10 healthy controls were prospectively studied. Sixty-two patients had symptoms refractory to proton pump inhibitors (PPI). All patients underwent esophageal biopsy...
October 17, 2017: Gut and Liver
https://www.readbyqxmd.com/read/29023914/melanocytic-naevi-with-perineurial-differentiation-a-distinctive-variant-of-neurotised-naevi-and-a-diagnostic-pitfall-with-desmoplastic-melanoma
#12
Ingrid Ferreira, Peter Kind, Ivo Van Den Berghe, Lucy Melly, Felix Offner, Jason L Hornick, Thomas Brenn
AIMS: Spindle cell differentiation is not an uncommon finding in common acquired naevi and may represent a form of neurotisation with Schwannian differentiation of melanocytes. Perineurial differentiation in this context appears to be distinctly rare and is only poorly documented in the literature. METHODS AND RESULTS: We have identified six melanocytic tumours showing spindle cell morphology and perineurial differentiation from routine and referral material. Clinical data and follow-up were obtained, and the histological and immunohistochemical features were analysed...
October 11, 2017: Histopathology
https://www.readbyqxmd.com/read/28994126/staphylococcus-aureus-v8-protease-disrupts-the-integrity-of-the-airway-epithelial-barrier-and-impairs-il-6-production-in-vitro
#13
Jae Murphy, Mahnaz Ramezanpour, Natalia Stach, Grzegorz Dubin, Alkis James Psaltis, Peter-John Wormald, Sarah Vreugde
OBJECTIVE: Staphylococcus aureus (S. aureus) infection is known to contribute to the severity and recalcitrance of chronic rhinosinusitis (CRS), and its secreted products have been shown to alter the airway barrier. Extracellular proteases secreted by S. aureus are thought to be important in epithelial infection and immune evasion; however, their effect on airway mucosal barrier function is not known. METHODS: To investigate the impact of extracellular proteases on airway epithelial integrity, the purified S...
October 10, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28992023/garlic-diallyl-disulfide-and-diallyl-trisulfide-mitigates-effects-of-pro-oxidant-induced-cellular-stress-and-has-immune-modulatory-function-in-lps-stimulated-porcine-epithelial-cells
#14
N Horn, G Miller, K M Ajuwon, O Adeola
The objective of the current study was to determine if garlic-derived diallyl disulfide (DADS) and diallyl trisulfide (DATS) could mitigate oxidative and endotoxin stress, using an intestinal porcine epithelial cell (IPEC-J2) model. The experiment was arranged as a 2 × 2 × 2 factorial of DADS + DATS (0 or 18 µM), pro-oxidant stressor (hydrogen peroxide at 0 or 100 µM), and endotoxin stressor (lipopolysaccharide [LPS] at 0 or 10 µg/mL) with 8 replicates per treatment. Cells were incubated with DADS + DATS for 18 h, LPS for 6 h, then with hydrogen peroxide for 3 h...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28988777/delta-toxin-from-clostridium-perfringens-perturbs-intestinal-epithelial-barrier-function-in-caco-2-cell-monolayers
#15
Soshi Seike, Masaya Takehara, Teruhisa Takagishi, Kazuaki Miyamoto, Keiko Kobayashi, Masahiro Nagahama
Clostridium perfringens delta-toxin is a β-barrel-pore-forming toxin (β-PFT) and a presumptive virulence factor of type B and C strains, which are causative organisms of fatal intestinal diseases in animals. We showed previously that delta-toxin causes cytotoxicity via necrosis in sensitive cells. Here, we examined the effect of delta-toxin on intestinal membrane integrity. Delta-toxin led to a reduction in transepithelial electrical resistance (TEER) and increased the permeability of fluorescence isothiocyanate-conjugated dextran in human intestinal epithelial Caco-2 cells without changing the tight junction proteins, such as zonula occludens-1 (ZO-1), occludin, and claudin-1...
October 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28978646/lactate-mediated-mitoribosomal-defects-impair-mitochondrial-oxidative-phosphorylation-and-promote-hepatoma-cell-invasiveness
#16
Young-Kyoung Lee, Jin J Lim, Un-Woo Jeoun, Seongki Min, Eun-Beom Lee, So Mee Kwon, Changhan Lee, Gyesoon Yoon
Impaired mitochondrial oxidative phosphorylation (OXPHOS) capacity, accompanied by enhanced glycolysis, is a key metabolic feature of cancer cells, but its underlying mechanism remains unclear. Previously, we reported that human hepatoma cells that harbor OXPHOS defects exhibit high tumor cell invasiveness via elevated claudin-1 (CLN1). In the present study, we show that OXPHOS-defective hepatoma cells (SNU354 and SNU423 cell lines) exhibit reduced expression of mitochondrial ribosomal protein L13 (MRPL13), a mitochondrial ribosome (mitoribosome) subunit, suggesting a ribosomal defect...
October 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28967978/topical-glucose-induces-claudin-1-and-filaggrin-expression-in-a-mouse-model-of-atopic-dermatitis-and-in-keratinocyte-culture-exerting-anti-inflammatory-effects-by-repairing-skin-barrier-function
#17
Kiyoko Yamada, Kenji Matsushita, Wang Jingshu, Takuro Kanekura
Atopic dermatitis (AD) is a chronic inflammatory skin disease. Corticosteroids, which are widely used for AD treatment, have adverse effects, and alternative treatments are urgently needed. This study examined the effect of topical application of high-dose glucose on inflamed skin in a murine model of AD. High-dose glucose treatment on the ear reduced dermatitis scores and ear thicknesses in mite antigen-treated NC/Nga mice. The levels of thymus and activation-regulated chemokine (TARC), Th cytokines (interleukin (IL)-4, IL-5, IL-12, IL-13, and (interferon) IFN-γ), and IgE were decreased in the serum of high-dose glucose-treated mice...
October 2, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/28967086/intestinal-mucosal-barrier-dysfunction-in-sap-patients-with-mods-ameliorated-by-continuous-blood-purification
#18
Qing Shen, Zhengrong Li, Shanshan Huang, Liman Li, Hua Gan, Xiao-Gang Du
BACKGROUND: Dysfunction of the intestinal mucosal barrier plays an important role in the pathophysiology of severe acute pancreatitis (SAP). Continuous blood purification (CBP) has been shown to improve the prognosis of SAP patients. In order to investigate the effect of CBP on intestinal mucosal barrier dysfunction in SAP patients with MODS, we conducted in vivo and in vitro experiments to explore the underlying mechanisms. METHODS: The markers for the assessment of intestinal mucosal barrier function including serum diamine oxidase (DAO), endotoxin and intestinal epithelial monolayer permeability were detected during CBP therapy...
September 14, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28946937/airborne-particulate-matter-increases-muc5ac-expression-by-downregulating-claudin-1-expression-in-human-airway-cells
#19
Sang-Su Kim, Cheol Hong Kim, Ji Wook Kim, Hsi Chiang Kung, Tae Woo Park, Yu Som Shin, Ju Deok Kim, Siejeong Ryu, Wang-Joon Kim, Yung Hyun Choi, Kyoung Seob Song
CLB2.0, a constituent of PM, induces secretion of multiple cytokines and chemokines that regulate airway inflammation. Specifically, IL-6 upregulates CLB2.0-induced MUC5AC and MUC1 expression. Interestingly, of the tight junction proteins examined, claudin-1 expression was inhibited by CLB2.0. While the overexpression of claudin-1 decreased CLB2.0-induced MUC5AC expression, it increased the expression of the anti-inflammatory mucin, MUC1. CLB2.0-induced IL-6 secretion was mediated by ROS. The ROS scavenger N-acetyl-cysteine inhibited CLB2...
September 26, 2017: BMB Reports
https://www.readbyqxmd.com/read/28935573/acrolein-disrupts-tight-junction-proteins-and-causes-endoplasmic-reticulum-stress-mediated-epithelial-cell-death-leading-to-intestinal-barrier-dysfunction-and-permeability
#20
Wei-Yang Chen, Min Wang, Jingwen Zhang, Shirish S Barve, Craig J McClain, Swati Joshi-Barve
Increasing evidence suggests that environmental and dietary factors can affect intestinal epithelial integrity leading to gut permeability and bacterial translocation. Intestinal barrier dysfunction is a pathogenic process associated with many chronic disorders. Acrolein is an environmental and dietary pollutant and a lipid-derived endogenous metabolite. The impact of acrolein on the intestine has not been investigated before and is evaluated in this study, both in vitro and in vivo. Our data demonstrate that oral acrolein exposure in mice caused damage to the intestinal epithelial barrier, resulting in increased permeability and subsequently translocation of bacterial endotoxin-lipopolysaccharide into the blood...
September 19, 2017: American Journal of Pathology
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