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Claudin-1

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https://www.readbyqxmd.com/read/28096546/ma026-an-anti-hepatitis-c-virus-compound-opens-tight-junctions-of-the-epithelial-cell-membrane
#1
Yusuke Kanda, Youhei Yamasaki, Satomi Shimura, Shinji Kamisuki, Fumio Sugawara, Yoko Nagumo, Takeo Usui
MA026 is an antiviral natural compound against hepatitis C virus (HCV). It was recently reported that MA026 binds claudin-1 (CLDN1) and inhibits HCV infection. Although CLDN1 is an important component of tight junctions (TJ) in the epithelial cell layer, the effects of MA026 on the TJ barrier function remained to be revealed. Here we report that MA026 irreversibly opens the TJ. MA026 irreversibly increased FD4 permeability and decreased transepithelial electrical resistance (TER) for at least 5 h. Although MA026 increased Ca(2+) influx in layered MDCKII cells, the Ca(2+) influx was less than that of capsaicin, a reversible TJ opener...
January 18, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28091645/yogurt-inhibits-intestinal-barrier-dysfunction-in-caco-2-cells-by-increasing-tight-junctions
#2
Kelley K Putt, Ruisong Pei, Heather M White, Bradley W Bolling
Chronic inflammation disrupts intestinal barrier function and may contribute to the pathology of obesity and other diseases. The goal of this study was to determine the mechanism by which yogurt improves intestinal barrier function. Caco-2 cells were differentiated on Transwell inserts and used as a model of intestinal barrier permeability. Transepithelial electrical resistance (TEER) and flux of 4 kDa fluorescein isothiocyanate-dextran (FD) and lucifer yellow (LY) were used as indicators of monolayer integrity and paracellular permeability...
January 16, 2017: Food & Function
https://www.readbyqxmd.com/read/28090006/expression-of-apical-junction-complex-proteins-in-colorectal-mucosa-of-miniature-dachshunds-with-inflammatory-colorectal-polyps
#3
Nozomu Yokoyama, Hiroshi Ohta, Yumiko Kagawa, Rommaneeya Leela-Arporn, Angkhana Dermlim, Khoirun Nisa, Tomoya Morita, Tatsuyuki Osuga, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Mitsuyoshi Takiguchi
We examine the expression of tight junction and adherence junction proteins in the colorectal mucosa of miniature dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). Colorectal mucosa samples were endoscopically obtained from 8 MDs with ICRPs and 8 control dogs for immunoblotting. Paraffin-embedded tissues of surgically resected inflamed lesions from another 5 MDs with ICRPs and full-thickness colorectal specimens from 5 healthy beagles were obtained for immunohistochemistry. The expression patterns of claudin-1, -2, -3, -4, -5, -7 and -8, E-cadherin and β-catenin were analyzed in the non-inflamed mucosa and inflamed mucosa of ICRPs and colorectal mucosa of control dogs by immunoblotting...
January 16, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28067896/pathogenic-role-of-endogenous-tnf-%C3%AE-in-the-development-of-sj%C3%A3-gren-s-like-sialadenitis-and-secretory-dysfunction-in-non-obese-diabetic-mice
#4
Jing Zhou, Toshihisa Kawai, Qing Yu
Patients with Sjögren's syndrome (SS), an autoimmune disease primarily affecting exocrine glands, exhibit enhanced TNF-α expression in the saliva and salivary glands. However, the precise in vivo role of TNF-α during the initiation and development of SS is not clearly defined. The present study is undertaken to determine the function of endogenously produced TNF-α in the pathogenesis of SS in non-obese diabetic (NOD) mice, a model of this human disease. Administration of a neutralizing anti-TNF-α antibody to female NOD mice during the stage prior to disease onset significantly improved salivary secretion, indicating a remission of clinical symptoms of SS...
January 9, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28052271/establishment-and-characterization-of-an-sv40-large-t-antigen-transduced-porcine-colonic-epithelial-cell-line
#5
Bastian Kaiser, Martina Böttner, Thilo Wedel, Ronald M Brunner, Tom Goldammer, Szilvia Lesko, Gotthold Gäbel, Alexander Gleich, Helga Pfannkuche
Continuous cell lines have become indispensable tools that have enabled investigations into cellular mechanisms by increasing experimental reproducibility and sample availability, and decreasing the use of experimental animals. To facilitate studies of epithelial barrier function of the porcine colon, we aimed to establish an epithelial cell line with an extended replicative capacity. Cells were isolated from the proximal colon of a 3-week-old piglet and transduced using a recombinant retroviral vector construct containing the simian virus 40 large T antigen (SV40 TAg)...
January 5, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28038359/green-tea-extract-treatment-reduces-nf%C3%AE%C2%BAb-activation-in-mice-with-diet-induced-nonalcoholic-steatohepatitis-by-lowering-tnfr1-and-tlr4-expression-and-ligand-availability
#6
Jinhui Li, Teryn N Sapper, Eunice Mah, Meredith V Moller, Joshua B Kim, Chureeporn Chitchumroonchokchai, Joshua D McDonald, Richard S Bruno
NFκB-mediated inflammation contributes to liver injury during nonalcoholic steatohepatitis (NASH). We hypothesized that antiinflammatory activities of green tea extract (GTE) during NASH would lower tumor necrosis factor receptor-1 (TNFR1)- and Toll-like receptor-4 (TLR4)-mediated NFκB activation. Male C57BL6/J mice (6 weeks old) were fed a low-fat (LF) or high-fat (HF) diet for 12 weeks to induce NASH. They were then randomized to continue on these diets supplemented with 0 or 2% GTE (n=10/group) for an additional 8 weeks prior to evaluating NASH, NFκB inflammation and TNFR1 and TLR4 receptor complexes and their respective ligands, TNFα and endotoxin...
December 21, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28034640/oral-perineurioma-clinicopathologic-features-from-two-cases-and-review-of-literature
#7
Wagner Gomes da Silva, Marisol Martínez Martínez, Águida Maria Menezes Aguiar Miranda, Roberto Bastos Silva, Henrique Martins da Silveira, Oslei Paes de Almeida, Fábio Ramôa Pires
The present study reports two cases of oral perineuriomas, including a clinicopathologic review of the 39 published cases (17 intraneural and 22 extraneural perineuriomas) in the English language literature. In the first case, the tumor occurred in an 84-year-old male as a painless, asymptomatic, 20-mm submucosal nodule on the right lower mucobuccal fold near to the premolar area. In the second case, a 46-year-old female presented with a painless, 25-mm, slow-growing, fibrous, pedunculated nodule on the right buccal mucosa...
October 12, 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/27999452/microrna-regulation-of-endothelial-junction-proteins-and-clinical-consequence
#8
REVIEW
Yugang Zhuang, Hu Peng, Victoria Mastej, Weiguo Chen
Cellular junctions play a critical role in structural connection and signal communication between cells in various tissues. Although there are structural and functional varieties, cellular junctions include tight junctions, adherens junctions, focal adhesion junctions, and tissue specific junctions such as PECAM-1 junctions in endothelial cells (EC), desmosomes in epithelial cells, and hemidesmosomes in EC. Cellular junction dysfunction and deterioration are indicative of clinical diseases. MicroRNAs (miRNA) are ~20 nucleotide, noncoding RNAs that play an important role in posttranscriptional regulation for almost all genes...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27981377/myrrh-exerts-barrier-stabilising-and-protective-effects-in-ht-29-b6-and-caco-2-intestinal-epithelial-cells
#9
Rita Rosenthal, Julia Luettig, Nina A Hering, Susanne M Krug, Uwe Albrecht, Michael Fromm, Jörg-Dieter Schulzke
PURPOSE: Myrrh, the oleo-gum resin of Commiphora molmol, is well known for its anti-inflammatory properties. In different animal models, it protected against DSS-, TNBS- and oxazolone-induced colitis. To date, no information concerning the effect of myrrh on barrier properties are available. Thus, this study investigates the effect of myrrh on paracellular barrier function in the absence or presence of the pro-inflammatory cytokine TNFα. METHODS: Monolayers of human colon cell lines HT-29/B6 and Caco-2 were incubated with myrrh under control conditions or after challenge with the pro-inflammatory cytokine TNFα...
December 15, 2016: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/27974683/cldn1-expression-in-cervical-cancer-cells-is-related-to-tumor-invasion-and-metastasis
#10
Wei-Na Zhang, Wei Li, Xiao-Li Wang, Zheng Hu, Da Zhu, Wen-Cheng Ding, Dan Liu, Ke-Zhen Li, Ding Ma, Hui Wang
Even though infection with human papillomaviruses (HPV) is very important, it is not the sole cause of cervical cancer. Because it is known that genetic variations that result from HPV infection are probably the most important causes of cervical cancer, we used human whole genome array comparative genomic hybridization to detect the copy number variations of genes in cervical squamous cell carcinoma. The results of the array were validated by PCR, FISH and immunohistochemistry. We find that the copy number and protein expression of claudin-1 (CLDN1) increase with the progression of cervical cancer...
December 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27966776/tumor-necrosis-factor-%C3%AE-increases-claudin-1-4-and-7-expression-in-tubular-cells-role-in-permeability-changes
#11
Yasaman Amoozadeh, Qinghong Dan, Shaista Anwer, Hsiao Han Huang, Vanessa Barbieri, Faiza Waheed, Mazharul Maishan, Katalin Szászi
Tumor necrosis factor-α (TNFα), is a pathogenic cytokine in kidney disease that alters expression of claudins in tubular cells. Previously we showed that in LLC-PK1 cells TNFα caused a biphasic change in transepithelial resistance (TER) consisting of an early drop and recovery, followed by a late increase. However, the underlying mechanisms and the role of specific claudins in the TER effect remained incompletely understood. Here we sought to define how TNFα affects claudins 1, 4 and 7 in tubular cells and to correlate their changes with the TER effect...
December 14, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27957611/barrier-function-in-the-peripheral-and-central-nervous-system-a-review
#12
REVIEW
A K Reinhold, H L Rittner
The peripheral (PNS) and central nervous system (CNS) are delicate structures, highly sensitive to homeostatic changes-and crucial for basic vital functions. Thus, a selection of barriers ensures the protection of the nervous system from noxious blood-borne or surrounding stimuli. In this chapter, anatomy and functioning of the blood-nerve (BNB), the blood-brain (BBB), and the blood-spinal cord barriers (BSCB) are presented and the key tight junction (TJ) proteins described: claudin-1, claudin-3, claudin-5, claudin-11, claudin-12, claudin-19, occludin, Zona occludens-1 (ZO-1), and tricellulin are by now identified as relevant for nerval barriers...
January 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27951472/soy-protein-concentrate-mitigates-markers-of-colonic-inflammation-and-loss-of-gut-barrier-function-in-vitro-and-in-vivo
#13
Zachary T Bitzer, Amy L Wopperer, Benjamin J Chrisfield, Ling Tao, Timothy K Cooper, Jairam Vanamala, Ryan J Elias, John E Hayes, Joshua D Lambert
Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability...
November 27, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27924224/micu1-may-be-a-promising-intervention-target-for-gut-derived-sepsis-induced-by-intra-abdominal-hypertension
#14
Yuxin Leng, Qinggang Ge, Zhiling Zhao, Kun Wang, Gaiqi Yao
Intra-abdominal hypertension (IAH) is a common and serious complication in critically ill patients, for which there is no targeted therapy. IAH-induced dysfunction of intestinal barriers is closely associated with oxidative imbalances, which are considered to provide a pathophysiological basis for subsequent gut-derived sepsis. However, the upstream mechanism that produces oxidative damage during IAH remains unknown. It is not clear whether 'mitochondrial Ca(2+) uptake 1' (MICU1, the key protein regulating the oxidative process) is involved in preventing Ca(2+)m (mitochondrial Ca(2+)) overload...
2016: Cell Death Discovery
https://www.readbyqxmd.com/read/27920472/anti-inflammatory-effect-of-cannabinoid-agonist-win55-212-on-mouse-experimental-colitis-is-related-to-inhibition-of-p38mapk
#15
Ya-Jing Feng, Yong-Yu Li, Xu-Hong Lin, Kun Li, Ming-Hua Cao
AIM: To investigate the anti-inflammatory effect and the possible mechanisms of an agonist of cannabinoid (CB) receptors, WIN55-212-2 (WIN55), in mice with experimental colitis, so as to supply experimental evidence for its clinical use in future. METHODS: We established the colitis model in C57BL/6 mice by replacing the animals' water supply with 4% dextran sulfate sodium (DSS) for 7 consecutive days. A colitis scoring system was used to evaluate the severity of colon local lesion...
November 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27914788/claudin-6-enhances-cell-invasiveness-through-claudin-1-in-ags-human-adenocarcinoma-gastric-cancer-cells
#16
A C Torres-Martínez, J F Gallardo-Vera, A N Lara-Holguin, L F Montaño, E P Rendón-Huerta
Claudins participate in tissue barrier function. The loss of this barrier is associated to metalloproteases-related extracellular matrix and basal membranes degradation. Claudin-1 is a pro-MMP-2 activator and claudin-6 transfected AGS (AGS-Cld6) cells are highly invasive. Our aim was to determine if claudin-6 was direct or indirectly associated with MMP-2 activation and cell invasiveness. Cytofluorometry, cell fractioning, immunoprecipitation, gelatin-zymography, cell migration and invasiveness assays were performed, claudin-2, -6, -7 and -9 transfected AGS cells, anti-MMP-2, -9 and -14, anti-claudins specific antibodies and claudin-1 small interfering RNA were used...
January 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/27904960/active-and-passive-involvement-of-claudins-in-the-pathophysiology-of-intestinal-inflammatory-diseases
#17
REVIEW
Christian Barmeyer, Michael Fromm, Jörg-Dieter Schulzke
Intestinal inflammatory diseases, four of which are discussed here, are associated with alterations of claudins. In ulcerative colitis, diarrhea and antigen entry into the mucosa occurs. Claudin-2 is upregulated but data on other claudins are still limited or vary (e.g., claudin-1 and -4). Apart from that, tight junction changes contribute to diarrhea via a leak flux mechanism, while protection against antigen entry disappears behind epithelial gross lesions (erosions) and apoptotic foci. Crohn's disease is additionally characterized by a claudin-5 and claudin-8 reduction which plays an active role in antigen uptake already before gross lesions appear...
January 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27883241/neutrophil-autophagy-and-extracellular-dna-traps-contribute-to-airway-inflammation-in-severe-asthma
#18
D L Pham, G-Y Ban, S-H Kim, Y S Shin, Y-M Ye, Y-J Chwae, H-S Park
BACKGROUND: Autophagy and neutrophil extracellular DNA traps (NETs) are implicated in asthma; however, their roles in asthma pathogenesis have not been elucidated. OBJECTIVES: We compared autophagy and NET production levels from peripheral blood neutrophils (PBNs) of patients with severe asthma (SA) and non-severe asthma (NSA). Additionally, we investigated the inflammatory effects of NETs on human airway epithelial cells (AECs) and peripheral blood eosinophils (PBEs)...
January 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27880979/low-dose-lipopolysaccharide-causes-biliary-injury-by-blood-biliary-barrier-impairment-in-a-rat-hepatic-ischemia-reperfusion-model
#19
J Reiling, K R Bridle, M Gijbels, F G Schaap, L Jaskowski, N Santrampurwala, L J Britton, C M Campbell, S W M Olde Damink, D H G Crawford, C H C Dejong, J Fawcett
INTRODUCTION: This study explored whether bacterial endotoxins, in the form of lipopolysaccharides (LPS), could have an injurious effect on the biliary tract in conjunction with ischemia. METHOD: Sixty-four rats were randomly assigned to four groups: sham operation (sham), 1mg/kg LPS i.p. (LPS), hepatic ischemia reperfusion (IR), and IR combined with LPS (IR+LPS). Following 1 or 6 hours of reperfusion, serum liver tests, bile duct histology, immunofluorescence microscopy (zonula occludens-1), bile composition (bile salts, phospholipids, lactate dehydrogenase), hepatic gene expression (bile salt transporters and inflammatory mediators) as well as serum and biliary cytokine concentrations were quantified and compared between the study groups...
November 23, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/27878684/bakuchiol-protects-against-acute-lung-injury-in-septic-mice
#20
Xinxin Zhang, Ning Chang, Yong Zhang, Mingxiang Ye, Zhiping Han, Jie Li, Jian Zhang
Sepsis is a systemic inflammatory reaction that may lead to multiple organ damage and acute lung injury (ALI). Bakuchiol (Bak) has been reported to confer protection against inflammation and oxidative stress. However, its effect on sepsis-induced acute lung injury remains unclear. In the present study, male C57BL/6 mice were subjected to cecal ligation and puncture (CLP), and Bak (15, 30, 60 mg/kg) was administered intragastrically after 0 and 3 h of surgery. Lung water content was detected. Pathologic changes in lung tissues were evaluated via hematoxylin and eosin (H&E) staining...
November 23, 2016: Inflammation
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