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Claudin-1

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https://www.readbyqxmd.com/read/29775701/lithium-posttreatment-alleviates-blood-brain-barrier-injury-after-intracerebral-hemorrhage-in-rats
#1
Weishan Li, Rui Li, Sha Zhao, Cheng Jiang, Zhen Liu, Xiaobo Tang
Vasogenic cerebral edema formation after blood brain barrier (BBB) damage aggravates the devastating consequences of intracerebral hemorrhage (ICH). The present study aims to probe into a therapeutic method on BBB preservation after ICH with a glycogen synthase kinase-3β (GSK-3β) inhibitor, lithium. Intrastriatal infusion of semicoagulated autologous whole blood or sham surgery was performed on male Sprague-Dawley (SD) rats (n = 208).Experimental animals received administration of 4,6-disubstitutedpyrrolo-pyrimidine (TWS119), lithium alone or in combination with a phosphatidylinositol 3-kinase inhibitor, wortmannin, after ICH...
May 15, 2018: Neuroscience
https://www.readbyqxmd.com/read/29773105/-protective-role-of-green-tea-polyphenols-in-intestinal-mucosal-barrier-function-of-mice-with-colitis-induced-by-tnbs-through-inhibiting-jak2-stat3-pathway
#2
Jin Xi, Sitang Ge, Lugen Zuo, Yuke Zhu, Lian Wang, Qiaoli Xie
Objective To analyze the therapeutic effect of green tea polyphenols (GTP) in mice with colitis induced by TNBS and its possible mechanism. Methods Colitis was induced by TNBS in BALB/C mice. The mice were randomly divided into GTP group (n=10) and TNBS model group (n=10). Mice in the GTP group were given GTP [0.2 mL at a dose level of 100 mg/(kg.d)] by oral gavage, and mice in the model group were given normal saline [0.2 ml/d] by gavage. Four weeks later, the mice were sacrificed. Disease activity index (DAI) and inflammatory score were evaluated by HE staining...
March 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29770867/dietary-supplementation-with-an-amino-acid-blend-enhances-intestinal-function-in-piglets
#3
Dan Yi, Baocheng Li, Yongqing Hou, Lei Wang, Di Zhao, Hongbo Chen, Tao Wu, Ying Zhou, Binying Ding, Guoyao Wu
The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB...
May 16, 2018: Amino Acids
https://www.readbyqxmd.com/read/29747061/serrated-epithelial-colorectal-polyps-hyperplastic-polyps-sessile-serrated-adenomas-with-perineurial-stroma-clinicopathological-and-molecular-analysis-of-a-new-series
#4
Katharina Erlenbach-Wünsch, Michel Bihl, Arndt Hartmann, Gabriel M Groisman, Michael Vieth, Abbas Agaimy
Serrated colorectal fibroblastic polyps (FPs) are rare benign mucosal lesions composed of serrated epithelial crypts separated and distorted by intimately associated bland spindle cell proliferations with perineurial-like phenotype. We herein describe 21 new FPs affecting 10 females and 9 males aged 45 to 80 yrs. (mean, 62 yrs). Lesions originated in the sigmoid colon/rectosigmoid junction (n = 16), rectum (n = 2), and other parts of the colon (n = 3). Most patients had additional synchronous or metachronous polyps: classical adenomas (12 patients), sessile serrated adenoma/SSA (1 patient), hyperplastic polyps/HPs (7 patients), both HPs and adenomas (6 patients) and colorectal cancer (2 patients)...
May 4, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29726306/secreted-giardia-intestinalis-cysteine-proteases-disrupt-intestinal-epithelial-cell-junctional-complexes-and-degrade-chemokines
#5
Jingyi Liu, Showgy Ma'ayeh, Dimitra Peirasmaki, Britta Lundström-Stadelmann, Lars Hellman, Staffan G Svärd
Giardiasis is a common diarrheal disease caused by the protozoan parasite Giardia intestinalis. Cysteine proteases (CPs) are acknowledged as virulence factors in Giardia but their specific role in the molecular pathogenesis of disease is not known. Herein, we aimed to characterize the three main secreted CPs (CP14019, CP16160 and CP16779), which were identified by mass spectrometry in the medium during interaction with intestinal epithelial cells (IECs) in vitro. First, the CPs were epitope-tagged and localized to the endoplasmic reticulum and cytoplasmic vesicle-like structures...
December 31, 2018: Virulence
https://www.readbyqxmd.com/read/29705796/clostridium-tyrobutyricum-protect-intestinal-barrier-function-from-lps-induced-apoptosis-via-p38-jnk-signaling-pathway-in-ipec-j2-cells
#6
Zhiping Xiao, Lujie Liu, Wenjing Tao, Xun Pei, Geng Wang, Minqi Wang
BACKGROUND/AIMS: The intestinal mucosa forms a physical and metabolic barrier against the diffusion of pathogens, toxins, and allergens from the lumen into the circulatory system. Early weaning, a critical phase in swine production, can compromise intestinal barrier function through mucosal damage and alteration of tight junction integrity Maintenance of intestinal barrier function plays a pivotal role in optimum gastrointestinal health. In this study, we investigated the effects of Clostridium tyrobutyricum (C...
April 25, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29705246/profilin-mediated-food-allergic-reactions-are-associated-with-oral-epithelial-remodeling
#7
Domenico Rosace, Cristina Gomez-Casado, Paloma Fernandez, Marina Pérez Gordo, María Del Carmen Dominguez, Angel Vega, María Teresa Belver, Tania Ramos, Francisco Vega, Guadalupe Marco, Manuel de Pedro, Leticia Sanchez, María de Las Mercedes Arnas, Marcela Santaolalla, Miguel Ángel Saez, Sara Benedé, Montserrat Fernandez-Rivas, Carlos Blanco, Maria Isabel Alvarado, María M Escribese, Domingo Barber
BACKGROUND: In areas of high exposure to grass pollen, allergic patients are frequently sensitized to profilin, and some develop severe profilin-mediated food reactions. This specific population of patients is ideal to study the relationship between respiratory and food allergies. OBJECTIVE: To determine the role of oral mucosa epithelial barrier integrity in profilin-mediated allergic reactions. METHODS: 38 profilin-allergic patients, stratified into mild or severe according to their clinical history and response to profilin challenge test, together with 6 non-allergic subjects were recruited...
April 26, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29699808/vitamin-a-and-retinoic-acid-exhibit-protective-effects-on-necrotizing-enterocolitis-by-regulating-intestinal-flora-and-enhancing-the-intestinal-epithelial-barrier
#8
Sa Xiao, Qiuping Li, Kun Hu, Yu He, Qing Ai, Liuhong Hu, Jialin Yu
BACKGROUND: Exaggerated inflammation that characterizes necrotizing enterocolitis (NEC) is caused by the invasion of pathogens through an immature intestinal barrier. Vitamin A (VA) and retinoic acid (RA) play important roles in the growth of epithelial tissue and in modulating immune function. OBJECTIVE: To investigate the roles of VA and RA in the development of NEC. METHODS: Levels of serum retinol in patients and in a NEC mouse model were detected with high-performance liquid chromatography...
April 23, 2018: Archives of Medical Research
https://www.readbyqxmd.com/read/29693599/dietary-supplementation-with-oleum-cinnamomi-improves-intestinal-functions-in-piglets
#9
Dan Yi, Qiuhong Fang, Yongqing Hou, Lei Wang, Haiwang Xu, Tao Wu, Joshua Gong, Guoyao Wu
The present study was to determine the efficacy of dietary supplementation with oleum cinnamomi (OCM) on growth performance and intestinal functions in piglets. Sixteen piglets (24-day-old) were randomly assigned to the control or OCM groups. Piglets in the control group were fed a basal diet, whereas piglets in the OCM group were fed the basal diet supplemented with 50 mg/kg OCM. On day 20 of the trial, blood samples and intestinal tissues were obtained from piglets. Compared with the control group, dietary OCM supplementation increased ( p < 0...
April 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29691121/stabilization-of-microtubules-restores-barrier-function-after-cytokine-induced-defects-in-reconstructed-human-epidermis
#10
Chiung-Yueh Hsu, Nicolas Lecland, Valérie Pendaries, Cécile Viodé, Daniel Redoulès, Carle Paul, Andreas Merdes, Michel Simon, Christiane Bierkamp
BACKGROUND: A variety of human skin disorders is characterized by defects in the epidermal barrier, leading to dehydration, itchiness, and rashes. Previously published literature suggests that microtubule stabilization at the cortex of differentiating keratinocytes is necessary for the formation of the epidermal barrier. OBJECTIVES: We tested whether stabilization of microtubules with paclitaxel or epothilone B can repair barrier defects that were experimentally induced in three-dimensional culture models of epidermis...
April 18, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29690739/-effects-of-short-chain-fatty-acid-on-barrier-disruption-of-human-intestinal-epithelial-cell-induced-by-endotoxin-lipopolysaccharide-and-the-related-mechanism
#11
Y H Feng, Y L Huang, P Wang, F J Wang
Objective: To investigate the effects of short chain fatty acid (SCFA) on barrier disruption of human intestinal epithelial cell induced by endotoxin/lipopolysaccharide (LPS) and the related mechanism. Methods: The human intestinal epithelial cell line Caco-2 was used to reproduce monolayer-cells. Cells were divided into control group, LPS group, and SCFA+ LPS group according to the random number table. Cells in control group were only routinely cultured with DMEM medium. Cells in LPS group were cultured with DMEM medium and LPS with final mass concentration of 10 μg/mL...
April 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29684479/impairment-of-the-gn%C3%AE-11-controlled-expression-of-claudin-1-and-mmp-9-and-collective-migration-of-human-breast-cancer-mcf-7-cells-by-dheas
#12
Neha Upmanyu, Ahmed Bulldan, Dimitrios Papadopoulos, Raimund Dietze, Viveka Nand Malviya, Georgios Scheiner-Bobis
Although dehydroepiandrosterone sulfate (DHEAS) constitutes the most abundant steroid in humans, in-depth investigations of its effects are rather scarce. We address here DHEAS effects on the estrogen receptor-positive metastatic human breast cancer cell line MCF-7. We focus on DHEAS-mediated signaling that might influence expression of claudin-1 and matrix metalloproteinase-9 (MMP-9), both known to be critical factors for migration and invasiveness of various cancers, including breast cancer cells. Physiological concentrations of DHEAS trigger persistent phosphorylation of Erk1/2 in MCF-7 cells...
April 20, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29680668/anti-il-7-receptor-%C3%AE-treatment-ameliorates-newly-established-sj%C3%A3-gren-s-like-exocrinopathy-in-non-obese-diabetic-mice
#13
Jing Zhou, Qing Yu
The levels of interleukin (IL)-7 and its receptor are elevated in the salivary glands of patients with Sjögren's syndrome (SS). Our previous study indicates that IL-7 plays a critical pathogenic role in the development and onset of SS in a mouse model of this disease. The present study aims at determining whether IL-7 also plays a role in sustaining SS pathologies after the disease onset, by using the non-obese diabetic (NOD) model. Intraperitoneal administration of a blocking antibody against the IL-7 receptor α chain (IL-7Rα) to female NOD mice aged 10 weeks, which exhibited newly onset clinical SS, for the duration of 3 weeks significantly ameliorated characteristic SS pathologies including hyposalivation and leukocyte infiltration of the submandibular glands (SMGs)...
April 19, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29678786/fetal-bovine-serum-induces-sustained-but-reversible-epithelial-mesenchymal-transition-in-the-beas-2b-cell-line
#14
S W Malm, E A Amouzougan, W T Klimecki
BEAS-2B is a non-malignant, immortalized human cell line that has been used extensively as a model of lung epithelium. Despite ATCC recommendations to culture BEAS-2B in defined, serum-free media, many publications describe culturing BEAS-2B in fetal bovine serum (FBS)-containing media. The objective of this study was to define the effects of FBS on BEAS-2B cells. FBS exposure resulted in increased nuclear levels of transcription factors responsible for regulating epithelial-mesenchymal transition (EMT), increased cell invasiveness and increased anchorage-independent growth...
April 17, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29675597/upregulated-epithelial-junction-expression-represents-a-novel-parameter-of-the-epithelial-radiation-response-to-fractionated-irradiation-in-oral-mucosa
#15
Sylvia Gruber, Nilsu Cini, Lisa-Marie Kowald, Julia Mayer, Andreas Rohorzka, Peter Kuess, Wolfgang Dörr
PURPOSE: During head and neck cancer treatment, the radiation response of the oral mucosa represents a frequent early side effect. Besides radiation-induced inhibition of proliferation, various other cellular responses occur. The radiation response of adherens and tight junction proteins was so far mostly investigated with large single-dose irradiation protocols, in vivo and in vitro. Therefore, the current study was initiated to investigate the impact of daily fractionated irradiation on the expression of adherens and tight junction proteins in vivo...
April 19, 2018: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/29670469/dietary-n-3-pufa-may-attenuate-experimental-colitis
#16
Cloé Charpentier, Ronald Chan, Emmeline Salameh, Khaly Mbodji, Aito Ueno, Moïse Coëffier, Charlène Guérin, Subrata Ghosh, Guillaume Savoye, Rachel Marion-Letellier
Background: Inflammatory bowel diseases (IBD) occurred in genetically predisposed people exposed to environmental triggers. Diet has long been suspected to contribute to the development of IBD. Supplementation with n-3 polyunsaturated fatty acids (PUFA) protects against intestinal inflammation in rodent models while clinical trials showed no benefits. We hypothesized that intervention timing is crucial and dietary fatty acid pattern may influence intestinal environment to modify inflammation genesis...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/29642727/lps-challenge-increased-intestinal-permeability-disrupted-mitochondrial-function-and-triggered-mitophagy-of-piglets
#17
Shuting Cao, Qianhui Zhang, ChunChun Wang, Huan Wu, Lefei Jiao, Qihua Hong, Caihong Hu
Here we investigated the influence of LPS-induced gut injury on antioxidant homeostasis, mitochondrial (mt) function and the level of mitophagy in piglets. The results showed that LPS-induced intestinal injury decreased the transepithelial electrical resistance, increased the paracellular permeability of F1TC dextran 4 kDa, and decreased the expression of claudin-1, occludin and zonula occludens-1 in the jejunum compared with the control group. LPS decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and increased the content of malondialdehyde in the jejunum...
January 1, 2018: Innate Immunity
https://www.readbyqxmd.com/read/29627063/high-fiber-dietary-and-sodium-butyrate-attenuate-experimental-autoimmune-hepatitis-through-regulation-of-immune-regulatory-cells-and-intestinal-barrier
#18
En-De Hu, Da-Zhi Chen, Jin-Lu Wu, Feng-Bin Lu, Lu Chen, Ming-Hua Zheng, Hui Li, Yu Huang, Ji Li, Xiao-Ya Jin, Yue-Wen Gong, Zhuo Lin, Xiao-Dong Wang, Lan-Man Xu, Yong-Ping Chen
Autoimmune hepatitis (AIH) is chronic autoimmune liver disease accompanied with the imbalance of Treg/Th17 and increased intestinal permeability. We investigated the effects of a high fiber diet and sodium butyrate on the Treg/Th17 and intestinal barrier function in an experimental autoimmune hepatitis. Intraperitoneal injection of hepatic antigen (S100) was used to induce experimental autoimmune hepatitis mice model and mice were divided into normal control, S100 model control, S100 plus high fiber diet and S100 plus sodium butyrate...
March 22, 2018: Cellular Immunology
https://www.readbyqxmd.com/read/29626645/chitosan-cmd-mediated-co-delivery-of-sn38-and-snail-specific-sirna-as-a-useful-anticancer-approach-against-prostate-cancer
#19
Amir Afkham, Leili Aghebati-Maleki, Homayoon Siahmansouri, Sanam Sadreddini, Majid Ahmadi, Sanam Dolati, Nahid Manafi Afkham, Parvin Akbarzadeh, Farhad Jadidi-Niaragh, Vahid Younesi, Mehdi Yousefi
BACKGROUND: Prostate cancer is known as the most common malignancy in men. Chitosan has generated great interest as a useful biopolymer for the encapsulation of small interfering RNA (siRNA). Due to cationic nature, chitosan is able to efficiently encapsulate siRNA molecules and form nanoparticles. Furthermore, the biocompatible and biodegradable attributes of chitosan have paved the way for its potential application in the in vivo delivery of therapeutic siRNAs. In this study, we aimed to design chitosan/CMD nanoparticles for the efficient encapsulation of the anti-cancer drugs SN38 and Snail-specific siRNA...
November 20, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29617867/weaning-disrupts-intestinal-antioxidant-status-impairs-intestinal-barrier-and-mitochondrial-function-and-triggers-mitophagy-in-piglets
#20
S T Cao, C C Wang, H Wu, Q H Zhang, L F Jiao, C H Hu
In the present study, we investigated the influence of weaning on antioxidant status, intestinal integrity, mitochondrial function, and the mitophagy level in piglets (weaned at 21 d) during the 1 wk after weaning. The redox status was measured by antioxidant enzymes activities, related genes expression, and malondialdehyde (MDA) content in jejunum. The intestinal barrier function was assessed by the Ussing chamber and expression of tight junction proteins in the jejunum. The function of intestine mitochondria was measured by mitochondrial DNA (mtDNA) content and activities of mitochondria oxidative phosphorylation complexes...
April 3, 2018: Journal of Animal Science
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