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Claudin-1

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https://www.readbyqxmd.com/read/28719888/inhibition-of-single-routes-of-intracellular-signaling-is-not-sufficient-to-neutralize-the-biphasic-disturbance-of-a-retinal-endothelial-cell-barrier-induced-by-vegf-a165
#1
Heidrun L Deissler, Gerhard K Lang, Gabriele E Lang
BACKGROUND/AIMS: Hallmark of diabetic macular edema is the enhanced permeability of retinal endothelial cells (REC) induced by vascular endothelial growth factor (VEGF-A165), which acts through activating specific receptors. To improve the predictability of inhibitors' potentials to block harmful effects of VEGF-A165, we investigated if its signaling pathways triggered in REC are redundant. METHODS: Immortalized bovine REC monolayers were treated with inhibitors specific for various protein kinases in combination with VEGF-A165...
July 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28700666/effects-of-kluyveromyces-marxianus-supplementation-on-immune-responses-intestinal-structure-and-microbiota-in-broiler-chickens
#2
Weiwei Wang, Zhui Li, Zengpeng Lv, Beibei Zhang, Hong Lv, Yuming Guo
To investigate the effects of Kluyveromyces marxianus on immune responses, intestinal structure and microbiota in broilers, 840 1-d-old broiler chicks were randomly divided into seven groups (eight replicates) and were fed basal diets without or with 0.25, 0.50, 1.0, 1.5, 2.0, and 2.5 g/kg of K. marxianus (2.0×1010 CFU/g). Serum and intestine samples were collected at 21 d of age. The results showed that increasing K. marxianus addition linearly reduced feed conversion ratio but linearly elevated relative thymus weight, as well as quadratically increased serum lysozyme and IgG levels, with the medium dose (1...
2017: PloS One
https://www.readbyqxmd.com/read/28699141/epi-perineural-and-schwann-cells-as-well-as-perineural-sheath-integrity-are-affected-following-2-4-d-exposure
#3
Marzieh Sharifi Pasandi, Farshad Hosseini Shirazi, Mohammad Reza Gholami, Hossein Salehi, Nowruz Najafzadeh, Mohammad Mazani, Hatef Ghasemi Hamidabadi, Ali Niapour
2,4-dicholorophenoxy acetic acid (2,4-D) is a worldwide-known hormone herbicide. However, there are increasing concerns about its exposure and risks of developing pathological conditions for the peripheral nervous system. The aim of this study was to investigate the mechanism(s) involved in the toxicity of 2,4-D on peripheral nerve's cellular components. The epi/perineural and Schwann cells and a total of three cell lines were treated with 2,4-D. The viability of cells at different doses of 2,4-D was measured by MTT assay...
July 11, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28688953/antrodia-camphorata-inhibits-metastasis-and-epithelial-to-mesenchymal-transition-via-the-modulation-of-claudin-1-and-wnt-%C3%AE-catenin-signaling-pathways-in-human-colon-cancer-cells
#4
You-Cheng Hseu, Yu-Hsien Chao, Kai-Yuan Lin, Tzong-Der Way, Hui-Yi Lin, Varadharajan Thiyagarajan, Hsin-Ling Yang
ETHNOPHARMACOLOGICAL RELEVANCE: Antrodia camphorata (AC) is a well known traditional Chinese medicinal mushroom in Taiwan, has been used to treat various diseases including cancer. MATERIALS AND METHODS: In this study, we investigated the anti-metastatic and anti-EMT properties of a fermented culture broth of AC in human colon SW480(claudin-1-) and metastatic SW620(claudin-1+) cancer cells in vitro. RESULTS: AC down-regulates claudin-1 and inhibits the proliferation and colony-formation abilities of both SW620(claudin-1+) and SW480(claudin-1-) cells...
July 5, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28681173/pro-apoptotic-effect-of-%C3%AE-2-tgz-in-claudin-1-low-triple-negative-breast-cancer-cells-involvement-of-claudin-1
#5
Geoffroy Marine, Kleinclauss Alexandra, Grandemange Stéphanie, Hupont Sébastien, Boisbrun Michel, Flament Stéphane, Grillier-Vuissoz Isabelle, Kuntz Sandra
PURPOSE: 40% of triple-negative breast cancer (TNBC) do not express claudin-1, a major constituent of tight junction. Patients with these "claudin-1-low" tumors present a higher relapse incidence. A major challenge in oncology is the development of innovative therapies for such poor prognosis tumors. In this context, we study the anticancer effects of ∆2-TGZ, a compound derived from troglitazone (TGZ), on cell models of these tumors. METHODS AND RESULTS: In MDA-MB-231 and Hs578T "claudin-1-low" TNBC cells, Δ2-TGZ treatment induced claudin-1 protein expression and triggered apoptosis as measured by FACS analysis (annexin V/PI co-staining)...
July 5, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28679754/sec24c-dependent-transport-of-claudin-1-regulates-hepatitis-c-virus-entry
#6
Peiqi Yin, Ye Li, Leiliang Zhang
Claudin-1 is an HCV co-receptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little is known about how the level of claudin-1 in cell surface is regulated by host vesicular transport. Here, we identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the COPII vesicular transport system. By interacting with Sec24C through its C-terminal YV, claudin-1 is transported from the ER and is eventually targeted into cell surface. Blocking COPII transport inhibits HCV entry by reducing the level of claudin-1 in cell surface...
July 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28676351/bidirectional-brain-gut-interactions-and-chronic-pathological-changes-after-traumatic-brain-injury-in-mice
#7
Elise L Ma, Allen D Smith, Neemesh Desai, Lumei Cheung, Marie Hanscom, Bogdan A Stoica, David J Loane, Terez Shea-Donohue, Alan I Faden
OBJECTIVES: Traumatic brain injury (TBI) has complex effects on the gastrointestinal tract that are associated with TBI-related morbidity and mortality. We examined changes in mucosal barrier properties and enteric glial cell response in the gut after experimental TBI in mice, as well as effects of the enteric pathogen Citrobacter rodentium (Cr) on both gut and brain after injury. METHODS: Moderate-level TBI was induced in C57BL/6 mice by controlled cortical impact (CCI)...
July 1, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28674421/chronic-stress-and-intestinal-barrier-dysfunction-glucocorticoid-receptor-and-transcription-repressor-hes1-regulate-tight-junction-protein-claudin-1-promoter
#8
Gen Zheng, Gordon Victor Fon, Walter Meixner, Amy Creekmore, Ye Zong, Michael K Dame, Justin Colacino, Priya H Dedhia, Shuangsong Hong, John W Wiley
Chronic stress and elevated glucocorticoid hormone are associated with decreases in the intestinal epithelial tight junction protein claudin-1 (CLDN1). Human/rat CLDN1 promoters contain glucocorticoid response elements (GREs) and adjacent transcription repressor HES1 binding N-boxes. Notch signaling target HES1 expression was high and glucocorticoid receptor (NR3C1) low at the crypt base and the pattern reversed at the crypt apex. Chronic stress reduced overall rat colon HES1 and NR3C1 that was associated with CLDN1 downregulation...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28662272/campylobacter-fetus-impairs-barrier-function-in-ht-29-b6-cells-through-focal-tight-junction-alterations-and-leaks
#9
Roland Bücker, Susanne M Krug, Anja Fromm, Hans Linde Nielsen, Michael Fromm, Henrik Nielsen, Jörg-Dieter Schulzke
Infections by Campylobacter species are the most common foodborne zoonotic disease worldwide. Campylobacter jejuni and C. coli are isolated most frequently from human stool samples, but severe infections by C. fetus (Cf), which can cause gastroenteritis, septicemia, and abortion, are also found. This study aims at the characterization of pathological changes in Cf infection using an intestinal epithelial cell model. The Cf-induced epithelial barrier defects appeared earlier than those of avian Campylobacter species like C...
June 29, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28659146/antibody-targeting-of-claudin-1-as-a-potential-colorectal-cancer-therapy
#10
S Cherradi, A Ayrolles-Torro, N Vezzo-Vié, N Gueguinou, V Denis, E Combes, F Boissière, M Busson, L Canterel-Thouennon, C Mollevi, M Pugnière, F Bibeau, M Ychou, P Martineau, C Gongora, M Del Rio
BACKGROUND: Metastatic colorectal cancer (mCRC) is one of the major causes of cancer-related death. Despite the substantial progress in mCRC management, it remains important to identify new therapeutic options and biological markers for personalized medicine. Here, we investigated the expression of claudin-1 (CLDN1), a major tight junction transmembrane protein, in the different colorectal cancer (CRC) molecular subtypes and then assessed the anti-tumor effect of a new anti-CLDN1 monoclonal antibody (mAb)...
June 28, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28651246/development-of-a-perfusion-platform-for-dynamic-cultivation-of-in-vitro-skin-models
#11
Kay Strüver, Wolfgang Friess, Sarah Hedtrich
Reconstructed skin models are suitable test systems for toxicity testing and for basic investigations on (patho-)physiological aspects of human skin. Reconstructed human skin, however, has clear limitations such as the lack of immune cells and a significantly weaker skin barrier function compared to native human skin. Potential reasons for the latter might be the lack of mechanical forces during skin model cultivation which is performed classically in static well-plate setups. Mechanical forces and shear stress have a major impact on tissue formation and, hence, tissue engineering...
June 27, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28650927/dexamethasone-prevents-lipopolysaccharide-induced-epithelial-barrier-dysfunction-in-rat-ileum
#12
Aline Barbosa Ribeiro, Humberto Giusti, Ana Paula Trevelin Souza, Celso Rodrigues Franci, Rafael Simone Saia
Inflammatory mediators have been postulated as elementary inducing factors to the disruption of the intestinal tight junctions (TJ) and consequently, gut permeability and bacterial translocation. Corticosteroids are considered the mainstay in the treatment of septic shock; however the impact of this therapy on the intestinal epithelial barrier dysfunction during septic shock remains unknown. Our aims were to demonstrate the role of low dexamethasone (DEX) doses in modulation of the inflammatory response, as well as the expression and the arrangement of TJ proteins in endotoxemic rats...
June 23, 2017: Shock
https://www.readbyqxmd.com/read/28650689/time-and-dose-dependent-claudin-contribution-to-biological-functions-lessons-from-claudin-1-in-skin
#13
Reitaro Tokumasu, Atsushi Tamura, Sachiko Tsukita
There are many types of biologic compartments in the body. Tight junctions (TJs) function to create a dynamic paracellular barrier to separate these compartments and maintain homeostasis. The TJ component claudin-1 is the major transmembrane protein responsible for forming the paracellular barrier in the epidermis; other claudins in the epidermis include claudin-3, -4, -12, -23 and -25. Accumulating evidence points to a relationship between claudin-1 and skin diseases; for example, a decrease in claudin-1 is reported in human atopic dermatitis...
June 19, 2017: Tissue Barriers
https://www.readbyqxmd.com/read/28636801/water-channels-and-barriers-formed-by-claudins
#14
REVIEW
Rita Rosenthal, Dorothee Günzel, Dian Theune, Carolina Czichos, Jörg-Dieter Schulzke, Michael Fromm
Physiological studies in leaky epithelia, like kidney proximal tubules and the small intestine, have documented water transport via both transcellular and paracellular pathways. The discovery of aquaporin water channels provided a molecular basis for transcellular water movement. In contrast, the contribution, or even existence, of a specific paracellular water pathway has been disputed for a long time, until the cation channel-forming tight junction protein claudin-2 was shown to also permit the paracellular passage of water through its pore...
June 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28633471/development-of-a-chicken-ileal-explant-culture-model-for-measurement-of-gut-inflammation-induced-by-lipopolysaccharide
#15
Qian Zhang, Susan D Eicher, Kolapo M Ajuwon, Todd J Applegate
Gut mucosa holds a single layer of epithelial cells and the largest mass of lymphoid tissue in the body. Although the epithelial cell culture model is widely used to assess intestinal barrier function, it has limitations for studying cellular interactions, in particular those of the immune system. In this study, a chicken ileal explant culture model was developed for investigating short-term gut inflammatory and secretory responses in an ex vivo environment. Initially, ileal explants from broilers at 21 d of age were cultured ex vivo up to 6 h...
June 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28633193/trictide-a-tricellulin-derived-peptide-to-overcome-cellular-barriers
#16
Jimmi Cording, Basak Arslan, Christian Staat, Sophie Dithmer, Susanne M Krug, Anneliese Krüger, Philipp Berndt, Ramona Günther, Lars Winkler, Ingolf E Blasig, Reiner F Haseloff
The majority of tight junction (TJ) proteins restrict the paracellular permeation of solutes via their extracellular loops (ECLs). Tricellulin tightens tricellular TJs (tTJs) and regulates bicellular TJ (bTJ) proteins. We demonstrate that the addition of recombinantly produced extracellular loop 2 (ECL2) of tricellulin opens cellular barriers. The peptidomimetic trictide, a synthetic peptide derived from tricellulin ECL2, increases the passage of ions, as well as of small and larger molecules up to 10 kDa, between 16 and 30 h after application to human epithelial colorectal adenocarcinoma cell line 2...
June 20, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28617312/autocrine-human-growth-hormone-promotes-invasive-and-cancer-stem-cell-like-behavior-of-hepatocellular-carcinoma-cells-by-stat3-dependent-inhibition-of-claudin-1-expression
#17
Yi-Jun Chen, Ming-Liang You, Qing-Yun Chong, Vijay Pandey, Qiu-Shi Zhuang, Dong-Xu Liu, Lan Ma, Tao Zhu, Peter E Lobie
Despite progress in diagnosis and treatment of hepatocellular carcinoma (HCC), the clinical outcome is still unsatisfactory. Increased expression of human growth hormone (hGH) in HCC has been reported and is associated with poor survival outcome in HCC patients. Herein, we investigated the mechanism of the oncogenic effects of hGH in HCC cell lines. In vitro functional assays demonstrated that forced expression of hGH in these HCC cell lines promoted cell proliferation, cell survival, anchorage-independent growth, cell migration, and invasion, as previously reported...
June 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28602912/synthetic-8-hydroxydeoxyguanosine-inhibited-metastasis-of-pancreatic-cancer-through-concerted-inhibitions-of-erm-and-rho-gtpase
#18
Jong-Min Park, Young-Min Han, Migyeong Jeong, Myung Hee Chung, Chang Il Kwon, Kwang Hyun Ko, Ki Baik Hahm
8-hydroxydeoxyguanosine (8-OHdG) is generated consequent to oxidative stress, but its paradoxical anti-oxidative, anti-inflammatory, and anti-mutagenic effects via Rho-GTPase inhibition were noted in various models of inflammation and cancer. Metastasis occurs through cell detachment, epithelial-mesenchymal transition (EMT), and cell migration; during these processes, changes in cell morphology are initiated through Rho-GTPase-dependent actin cytoskeleton polymerization. In this study, we explored the anti-metastatic mechanisms of 8-OHdG in Panc-1 pancreatic cancer cells...
June 8, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28598488/myosin-light-chain-kinase-knockout-improves-gut-barrier-function-and-confers-a-survival-advantage-in-polymicrobial-sepsis
#19
C Adam Lorentz, Zhe Liang, Mei Meng, Ching-Wen Chen, Benyam P Yoseph, Elise R Breed, Rohit Mittal, Nathan J Klingensmith, Alton B Farris, Eileen M Burd, Michael Koval, Mandy L Ford, Craig M Coopersmith
Sepsis-induced intestinal hyperpermeability is mediated by disruption of the epithelial tight junction, which is closely associated with the peri-junctional actin-myosin ring. Myosin light chain kinase (MLCK) phosphorylates the myosin regulatory light chain, resulting in increased permeability. The purpose of this study was to determine whether genetic deletion of MLCK would alter gut barrier function and survival from sepsis. MLCK(-/-) and wild type (WT) mice were subjected to cecal ligation and puncture and assayed for both survival and mechanistic studies...
June 7, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28594555/effect-of-aging-on-the-absorption-of-small-peptides-in-spontaneously-hypertensive-rats
#20
Vu Thi Hanh, Weilin Shen, Mitsuru Tanaka, Aino E Siltari, Riita A Korpela, Toshiro Matsui
In the present study, we aimed to evaluate the effect of aging on the absorption of small peptides in spontaneously hypertensive rats (SHRs). Three kinds of dipeptides, glycyl-sarcosine (Gly-Sar), Trp-His, and captopril (a dipeptidomimetic drug), a Gly-Sar-Sar tripeptide, a Gly-Sar-Sar-Sar tetrapeptide, and a Gly-Sar-Sar-Sar-Sar pentapeptide were administered at doses of 10 mg/kg each to 8- and 40-week-old SHRs. The peptides were all detected in their intact forms in the blood. There was a significantly promoted absorption of di-/tripeptides in aged SHRs compared with young SHRs...
June 8, 2017: Journal of Agricultural and Food Chemistry
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