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Indirect Antiglobulin Test

Alyssa Ziman, Claudia Cohn, Patricia M Carey, Nancy M Dunbar, Mark K Fung, Andreas Greinacher, Simon Stanworth, Nancy M Heddle, Meghan Delaney
BACKGROUND: Warm-reactive autoantibodies (WAAs) are the most common cause of autoimmune hemolytic anemia (AIHA) and can also be present without clinically significant hemolysis. WAAs complicate immunohematological testing, yet there is no commonly accepted approach to laboratory evaluation and red blood cell (RBC) selection. STUDY DESIGN AND METHODS: We searched PubMed/Cochrane Central for articles that described testing methodology and blood selection for patients with WAAs...
December 4, 2016: Transfusion
Sumaiya Adam, Hennie Lombaard
BACKGROUND: Minor red blood cell antibodies are becoming a more common cause of hemolytic disease of the newborn. Anti-U are a rare alloantibody found almost exclusively in people of black descent. There is limited experience to guide the management of pregnancies complicated by anti-U. Furthermore, there is often no suitable cross-matched blood available for transfusion of a patient with anti-U. CASE REPORT: A 21-year-old P0G1 presented at 25 weeks' gestation with D- disease in pregnancy...
September 23, 2016: Transfusion
Ashish Jain, Ajju Agnihotri, Neelam Marwaha, Ratti Ram Sharma
INTRODUCTION: Red cell allo- and auto-immunization is a well recognized problem in multi-transfused thalassemic patients. We conducted this study on 301 multi-transfused thalassemic patients under the Thalassemia Transfusion Programme of Advanced Pediatric Centre of PGIMER. AIMS AND OBJECTIVES: The study was designed to determine the frequency of alloimmunization and autoimmunization in multi-transfused thalassemic patients and to establish the specificity of alloantibody to red cell antigens, if alloimmunization is detected...
July 2016: Asian Journal of Transfusion Science
Siddhi P Shah, Sangeeta M Kalgutkar, Rajesh B Sawant, Anand S Deshpande
Anti-M antibody, which is not reactive at 37°C, is not clinically significant. Reports of clinically significant anti-M antibodies causing hemolytic disease of the fetus and the newborn (HDFN) and delayed hemolytic transfusion reaction (DHTR) are available. We report 13 cases of anti-M antibodies reactive at room temperature (RT) and at 37°C. These were found in patients of varied age groups (11 months to 85 years) with varied clinical diagnosis. All the female patients were multigravida. In all cases, antibody screening was positive at RT as well as at the indirect antiglobulin test (IAT) phase...
July 2016: Asian Journal of Transfusion Science
Mohammad Mehdi Akbarin, Hassan Ravari, Ataollah Rajabnejad, Narges Valizadeh, Bahare Fazeli
During a review of patients admitted with thromboangiitis obliterans (TAO), there was evidence of normochromic normocytic anemia and abrupt changes in hemoglobin (Hgb) levels in patients with several hospital admissions. Therefore, the evidence of hemolytic anemia was evaluated based on 37 banked plasma samples taken from Caucasian male TAO patients during disease exacerbation between 2012 and 2014. The patients' hospital records, including clinical manifestations and complete blood count, were evaluated. The following tests were performed on all samples: indirect antiglobulin test (IAT), C-reactive protein (CRP), high-sensitivity CRP (hsCRP), lactate dehydrogenase (LDH), haptoglobin, indirect bilirubin, d-aspartate aminotransferase (AST), and d-alanine aminotransferase (ALT)...
September 2016: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
Magalie P Tardy, Lauris Gastaud, Annick Boscagli, Frederic Peyrade, Andrea Gallamini, Antoine Thyss
The patients with refractory Hodgkin lymphoma have a poor prognosis. The nivolumab, an IgG4 monoclonal antibody inhibiting the program death 1 pathway has recently demonstrated its efficacy and its safety in patients with heavily pretreated refractory Hodgkin lymphoma. The side effects of this immunotherapy include autoimmune-like syndromes. A 75-year-old woman with no significant comorbidities was treated by nivolumab (3 mg/kg every 2 wk) as a third-line treatment for refractory Hodgkin lymphoma. A clinical response was observed with the first injection of nivolumab, with a reduction in superficial lymph nodes...
August 19, 2016: Hematological Oncology
L L Golovkina, A G Stremoukhova, T D Pushkina, R S Kalandarov, G V Atroshchenko, M N Vasil'eva, V L Surin, V V Salomashkina, O S Pshenichnikova, G Yu Miterev, E N Parovichnikova, V G Savchenko
AIM: to estimate the spread of weak D antigen types of the Rhesus system in the citizens of the Russian Federation and a possibility of serologically identifying these types. SUBJECTS AND METHODS: The red blood cells and DNA of people with weakened expression of D antigen were investigated using erythrocyte agglutination reaction in salt medium (2 methods); agglutination reaction in the gel columns containing IgM + IgG anti-D antibodies, indirect antiglobulin test with IgG anti-D antibodies (2 methods); polymerase chain reaction to establish the type of weak D...
2016: Terapevticheskiĭ Arkhiv
Rizwan Javed, Suvro Sankha Datta, Sabita Basu, Anupam Chakrapani
INTRODUCTION: Autoimmune hemolytic anemia can be classified depending on presence of warm, cold or mixed type of autoantibodies that are directed against antigens on the red blood cell surface. Here we report a case of pathological cold agglutinin disease which was eventually detected due to blood group discrepancy. CASE DETAILS: A request was sent to the blood bank for two units of packed red cells in a diagnosed case of CLL which showed type IV discrepancy during blood grouping...
June 2016: Indian Journal of Hematology & Blood Transfusion
D Swarup, P S Dhot, J Kotwal, A K Verma
BACKGROUND: Conventionally tube method is used for compatibility and cross matching in transfusion medicine. METHODS: A comparative study was carried out to evaluate the efficacy of conventional tube and gel technique. RESULT: Compatibility testing was performed on 1000 blood samples by conventional test tube method and DiaMed gel method. The results were analysed. CONCLUSION: The gel method was found to be a rapid and reliable procedure without controls...
April 2008: Medical Journal, Armed Forces India
Natasha Yeow, Heather McLiesh, Liyun Guan, Wei Shen, Gil Garnier
A rapid and simple paper-based elution assay for red blood cell antigen typing by the indirect antiglobulin test (IAT) was established. This allows to type blood using IgG antibodies for the important blood groups in which IgM antibodies do not exist. Red blood cells incubated with IgG anti-D were washed with saline and spotted onto the paper assay pre-treated with anti-IgG. The blood spot was eluted with an elution buffer solution in a chromatography tank. Positive samples were identified by the agglutinated and fixed red blood cells on the original spotting area, while red blood cells from negative samples completely eluted away from the spot of origin...
July 2016: Analytical and Bioanalytical Chemistry
N Burin des Roziers, C Ibanez, D Samuel, C Francoz, S Idri, A François, R Mortelecque, P Bierling, F Pirenne
A retrospective analysis was conducted on 20 D(-) liver transplant (LT) recipients transfused with D(+) RBCs perioperatively and screened for RBC antibodies between 2 and 6 months later. None developed anti-D detectable by the indirect antiglobulin test. Two patients produced weak anti-D that reacted only with papain-treated RBCs at 10 and 11 days without any sign of immune haemolysis. Antibodies became quickly undetectable. These data suggest an unusual pattern of alloimmunization in LT recipients with rapid, weak and transient antibody response and support the safety of transfusing D(+) RBCs in most of D(-) patients during LT surgery...
July 2016: Vox Sanguinis
E Ndahimana, A Gothot, C Gérard, F Senyana, S R'Zik, O Mukabayire, L Mutesa
BACKGROUND: Screening of alloantibodies in patients is not yet done in district hospitals of Rwanda. The practice is to transfuse ABO/D compatible blood following an immediate spin crossmatch (IS-XM) or indirect antiglobulin test crossmatch (IAT-XM). OBJECTIVES: To assess the risk of red blood cell (RBC) alloimmunisation associated with the use of IS-XM compared to the IAT-XM in patients receiving blood transfusions in district hospitals in Rwanda. DESIGN: A cross-sectional comparative descriptive study...
April 2013: East African Medical Journal
E Ndahimana, A Gothot, C Gérard, F Senyana, S R'Zik, O Mukabayire, L Mutesa
BACKGROUND: Screening of alloantibodies in patients is not yet done in district hospitals of Rwanda. The practice is to transfuse ABO/D compatible blood following an immediate spin crossmatch (IS-XM) or indirect antiglobulin test crossmatch (IAT-XM). OBJECTIVES: To assess the risk of red blood cell (RBC) alloimmunisation associated with the use of IS-XM compared to the IAT-XM in patients receiving blood transfusions in district hospitals in Rwanda. DESIGN: A cross-sectional comparative descriptive study...
April 2013: East African Medical Journal
Asmaa M Zahran, Khalid I Elsayh, Khaled Saad, Mostafa Embaby, Ahmed M Ali
BACKGROUND: β-Thalassemia major (BTM) is considered the most common hemoglobinopathy in Egypt and is one of the major health problems in our locality. MATERIALS & METHODS: We investigated the frequency of B-regulatory cells (CD19(+)CD38(hi)CD24(hi)); (Bregs) among polytransfused alloimmunized and non-alloimmunized children with BTM. The study included 110 polytransfused pediatric patients with β-thalassemia major. Clinical and transfusion records of all studied patients were reviewed...
March 2016: Blood Cells, Molecules & Diseases
M Studničková, I Holusková, V Durdová, T Kratochvílová, P Strašilová, I Marková, M Lubušký
AIM OF THE STUDY: Assess the incidence of spontaneous antepartal RhD alloimmunization in RhD negative pregnant women with an RhD positive fetus. DESIGN: Clinical study. SETTING: Department of Obstetrics and Gynecology, Medical School and University Hospital Olomouc. METHODS: A total of 906 RhD negative women with an RhD positive fetus and without the presence of anti-Dalloantibodies at the beginning of pregnancy were examined...
December 2015: Ceská Gynekologie
Ariane Sümnig, Beate Mayer, Volker Kiefel, Andreas Greinacher, Abdulgabar Salama
BACKGROUND: The phenomena of co-incidence of transfusion-induced allo- and autoantibodies, blockage and/or loss of red blood cell (RBC) antigens are conspicuous and may result in confusion and misdiagnosis. CASE REPORT: A 67-year-old female was transferred to the intensive care unit due to hemolysis which developed 2 days following transfusion of three Rh(D)-negative RBC units in the presence of strongly reactive autoantibodies. Standard serological testing and genotyping were performed...
September 2015: Transfusion Medicine and Hemotherapy
Xu Zhang, Changping Sun, Jianping Li
OBJECTIVE: To explore the molecular basis for an individual with a rare weak D phenotype. METHODS: Regular serological assaying and indirect antiglobulin testing (IAT) were performed to characterize the RhD blood group. Mutations of the RHD gene were screened by polymerase chain reaction (PCR), reverse transcription PCR and DNA sequencing. Amplified cDNA product was TA cloned and subjected to haplotype analysis. RESULTS: The RhD blood group of the proband was determined as weak D...
October 2015: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Swarupa Nikhil Bhagwat, Jayashree H Sharma, Julie Jose, Charusmita J Modi
CONTEXT: The routine immunohematological tests can be performed by automated as well as manual techniques. These techniques have advantages and disadvantages inherent to them. AIMS: The present study aims to compare the results of manual and automated techniques for blood grouping and crossmatching so as to validate the automated system effectively. MATERIALS AND METHODS: A total of 1000 samples were subjected to blood grouping by the conventional tube technique (CTT) and the automated microplate LYRA system on Techno TwinStation...
July 2015: Journal of Laboratory Physicians
Luciana Cayres Schmidt, Lilian Castilho, Otavio Vinicius Neves Vieira, Emília Sippert, Ane Caroline Gaspardi, Marina Lobato Martins, Maria Clara Fernandes da Silva Malta
BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated. METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique) were reevaluated for RhD status by three methods...
September 2015: Revista Brasileira de Hematologia e Hemoterapia
Dilek Gurlek Gokcebay, Abdurrahman Kara, Ikbal Ok Bozkaya, Vildan Culha, Nese Yarali, Bahattin Tunc, Namik Ozbek
Direct antiglobulin test positivity had been reported in the course of some lymphoproliferative neoplasms. However, there are a few case reports describing direct antiglobulin test (DAT) positivity in children with acute lymphoblastic leukemia (ALL). We herein report 8 patients who had positive DAT among 95 newly diagnosed children with ALL. None of these patients had evidence of hemolysis during the follow-up. An antibody was detected in 2 of 8 patients with positive DAT. These 2 children also had positive indirect antiglobulin test (IAT); an autoantibody that was reactive at 4°C, and an alloantibody (anti E) that was reactive at 37°C was detected...
2015: Pediatric Hematology and Oncology
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