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https://www.readbyqxmd.com/read/29343966/prevalence-of-antigliadin-iga-antibodies-in-psoriasis-vulgaris-and-response-of-seropositive-patients-to-a-gluten-free-diet
#1
Nikolai A Kolchak, Maria K Tetarnikova, Maria S Theodoropoulou, Alexandra P Michalopoulou, Demetrios S Theodoropoulos
Introduction: The course of psoriasis relies on a variety of metabolic and immunological parameters. Identification of underlying pro-inflammatory conditions and their control is desired for optimal management. Background: Increased prevalence of serum markers for celiac disease has been reported among patients with psoriasis. The likelihood of occult celiac disease in a subpopulation of patients has been postulated and gluten-free diets have been reported to be effective...
2018: Journal of Multidisciplinary Healthcare
https://www.readbyqxmd.com/read/29342175/immunocontraceptive-target-repertoire-defined-by-systematic-identification-of-sperm-membrane-alloantigens-in-a-single-species
#2
Nathaly Cormier, John J McGlone, John Leszyk, Daniel M Hardy
Sperm competence in animal fertilization requires the collective activities of numerous sperm-specific proteins that are typically alloimmunogenic in females. Consequently, sperm membrane alloantigens are potential targets for contraceptives that act by blocking the proteins' functions in gamete interactions. Here we used a targeted proteomics approach to identify the major alloantigens in swine sperm membranes and lipid rafts, and thereby systematically defined the repertoire of these sperm-specific proteins in a single species...
2018: PloS One
https://www.readbyqxmd.com/read/29338788/high-throughput-immunophenotypic-characterization-of-bone-marrow-and-cord-blood-derived-mesenchymal-stromal-cells-reveals-common-and-differentially-expressed-markers-identification-of-angiotensin-converting-enzyme-cd143-as-a-marker-differentially-expressed
#3
Eliana Amati, Omar Perbellini, Gianluca Rotta, Martina Bernardi, Katia Chieregato, Sabrina Sella, Francesco Rodeghiero, Marco Ruggeri, Giuseppe Astori
BACKGROUND: Mesenchymal stromal cells (MSC) are a heterogeneous population of multipotent progenitors used in the clinic because of their immunomodulatory properties and their ability to differentiate into multiple mesodermal lineages. Although bone marrow (BM) remains the most common MSC source, cord blood (CB) can be collected noninvasively and without major ethical concerns. Comparative studies comprehensively characterizing the MSC phenotype across several tissue sources are still lacking...
January 16, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29336363/utility-and-safety-of-intrathecal-methotrexate-treatment-in-severe-anti-n-methyl-d-aspartate-receptor-encephalitis-a-pilot-study
#4
Xun-Zhe Yang, Hua-Dong Zhu, Hai-Tao Ren, Yi-Cheng Zhu, Bin Peng, Li-Ying Cui, Hong-Zhi Guan
BACKGROUND: Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is a treatable autoimmune neurologic syndrome that occurs with or without tumor association. However, some severe cases are refractory to systemic immunotherapy. This pilot study aimed to evaluate the utility and safety of intrathecal methotrexate injection for severe patients with anti-NMDAR encephalitis who did not respond to first-line immunotherapy. METHODS: Intrathecal injections with methotrexate and dexamethasone were performed weekly in four legible patients within consecutive 4 weeks...
January 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29334288/targeted-anti-il-13-therapies-in-asthma-current-data-and-future-perspectives
#5
Polyxeni Ntontsi, Evgenia Papathanassiou, Stelios Loukides, Petros Bakakos, Georgios Hillas
Introduction The identification of patients with severe asthma who will benefit from a personalized management approach remains an unmet need. Interleukin-13 (IL-13) is a cytokine possessing a significant role in asthma pathogenesis and progression of disease. Humanised monoclonal antibodies against IL-13 and IL-13 and IL-4 receptors are mainly proposed as add-on therapy in patients with TH2-high inflammation with uncontrolled asthma despite maximum therapy. Areas covered The role of IL-13 in airway inflammation in severe asthma, the targeted anti-IL-13 therapies and biomarkers that predict response to anti-IL-13 treatment are discussed...
January 15, 2018: Expert Opinion on Investigational Drugs
https://www.readbyqxmd.com/read/29329556/cancer-immunotherapy-beyond-immune-checkpoint-inhibitors
#6
REVIEW
Julian A Marin-Acevedo, Aixa E Soyano, Bhagirathbhai Dholaria, Keith L Knutson, Yanyan Lou
Malignant cells have the capacity to rapidly grow exponentially and spread in part by suppressing, evading, and exploiting the host immune system. Immunotherapy is a form of oncologic treatment directed towards enhancing the host immune system against cancer. In recent years, manipulation of immune checkpoints or pathways has emerged as an important and effective form of immunotherapy. Agents that target cytotoxic T lymphocyte-associated molecule-4 (CTLA-4), programmed cell death receptor-1 (PD-1), and programmed cell death ligand-1 (PD-L1) are the most widely studied and recognized...
January 12, 2018: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/29326360/immunopet-in-inflammatory-bowel-disease-imaging-cd4-t-cells-in-a-murine-model-of-colitis
#7
Amanda Christine Freise, Kirstin A Zettlitz, Felix Bergara Salazar, Richard Tavaré, Wen-Ting K Tsai, Arion Hadjioannou, Nora Rozengurt, Jonathan Braun, Anna M Wu
Inflammatory bowel diseases (IBD) in humans are characterized in part by aberrant CD4+ T cell responses. Currently, identification of foci of inflammation within the gut requires invasive procedures such as colonoscopy and biopsy. Molecular imaging with antibody fragment probes could be used to noninvasively monitor cell subsets causing intestinal inflammation. Here, GK1.5 cys-diabody (cDb), an anti-mouse CD4 antibody fragment derived from the GK1.5 hybridoma, was used as a positron emission tomography (PET) probe for CD4+ T cells in the dextran sulfate sodium (DSS) mouse model of IBD...
January 11, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29324225/the-marburgvirus-neutralizing-human-monoclonal-antibody-mr191-targets-a-conserved-site-to-block-virus-receptor-binding
#8
Liam B King, Marnie L Fusco, Andrew I Flyak, Philipp A Ilinykh, Kai Huang, Bronwyn Gunn, Robert N Kirchdoerfer, Kathryn M Hastie, Amandeep K Sangha, Jens Meiler, Galit Alter, Alexander Bukreyev, James E Crowe, Erica Ollmann Saphire
Since their first identification 50 years ago, marburgviruses have emerged several times, with 83%-90% lethality in the largest outbreaks. Although no vaccines or therapeutics are available for human use, the human antibody MR191 provides complete protection in non-human primates when delivered several days after inoculation of a lethal marburgvirus dose. The detailed neutralization mechanism of MR191 remains outstanding. Here we present a 3.2 Å crystal structure of MR191 complexed with a trimeric marburgvirus surface glycoprotein (GP)...
January 10, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/29322650/molecular-characterization-of-the-%C3%AE-amyloid-4-10-epitope-of-plaque-specific-a%C3%AE-antibodies-by-affinity-mass-spectrometry-using-alanine-site-mutation
#9
Raluca Ștefănescu, Loredana Lupu, Marilena Manea, Roxana E Iacob, Michael Przybylski
Alzheimer disease is a neurodegenerative disease affecting an increasing number of patients worldwide. Current therapeutic strategies are directed to molecules capable to block the aggregation of the β-amyloid(1-42) (Aβ) peptide and its shorter naturally occurring peptide fragments into toxic oligomers and amyloid fibrils. Aβ-specific antibodies have been recently developed as powerful antiaggregation tools. The identification and functional characterization of the epitope structures of Aβ antibodies contributes to the elucidation of their mechanism of action in the human organism...
January 2018: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/29318959/novel-biological-therapies-in-severe-asthma-targeting-the-right-trait
#10
Gilda Varricchi, Giancarlo Marone, Giuseppe Spadaro, Michele Russo, Francescopaolo Granata, Arturo Genovese, Gianni Marone
Asthma is a heterogeneous disease characterized by chronic airway inflammation that results in a wide spectrum of clinical manifestations. Patients with severe asthma represent a substantial share of consumption of healthcare resources and hospitalization. Moreover, these patients are at risk of increased morbidity and mortality. Recently, several phenotypes and endotypes of asthma have been identified. The identification of specific subtypes of asthma is fundamental for optimizing the clinical benefit of novel treatments...
January 9, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29317885/seroprevalence-and-risk-factors-for-theileria-equi-infection-in-equines-from-khyber-pakhtunkhwa-province-pakistan
#11
Muhammad Jamal Khan Afridi, Abdul Hafeez Mian, Muhammad Saqib, Ghazanfar Abbas, Javid Ali, Muhammad Khalid Mansoor, Awais Ur Rahman Sial, Imaad Rasheed, Muhammad Hammad Hussain
Background: Theileria equi is a tick borne protozoan parasite which causes piroplasmosis among equines worldwide. The present study was aimed to determine seroprevalence of T. equi in donkeys, horses, and mules from two equine populated districts (Peshawar and Charsadda) of Khyber Pakhtunkhwa (KPK), Pakistan. Methods: A total of 393 equine (195 horses, 194 donkeys and 4 mules) serum samples were collected from five and four randomly selected localities in Charsadda (n = 193) and Peshawar (n = 200), respectively...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29317872/transmission-of-leishmania-infantum-by-rhipicephalus-sanguineus-acari-ixodidae-in-dogs
#12
Alaleh Rakhshanpour, Abdolali Malmasi, Mehdi Mohebali, Sedigheh Nabian, Hossein Mirhendi, Zabihollah Zarei, Abdolhossein Dalimi, Anita Mohammadiha, Behnaz Akhoundi, Amrollah Azarm
Background: Rhipicephalus sanguineus is the most widely distributed tick in the world, which is partly due to its biological flexibility and the global distribution of its major host, the domestic dog. In Mediterranean region it could be principal reservoir host for Leishmania infantum, usually transmitted by the phlebotomine sand flies. In this study, we evaluated the vector potential of R. sanguineus in transmitting L. infantum to uninfected dogs. Methods: During 2014, five dogs with clinical manifestations of canine visceral leishmaniasis (CVL), high anti-Leishmania antibody titers and tick infestation, were selected from CVL endemic areas (Tehran and Alborz provinces)...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29316248/a-standardized-flow-cytometry-procedure-for-the-monitoring-of-regulatory-t-cells-in-clinical-trials
#13
Pitoiset Fabien, Barbié Michèle, Monneret Guillaume, Braudeau Cécile, Pochard Pierre, Pellegrin Isabelle, Trauet Jacques, Labalette Myriam, Klatzmann David, Rosenzwajg Michelle
BACKGROUND: Quantification of regulatory T cells is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections and allergies. Human regulatory T cells are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29315487/using-extensional-flow-to-reveal-diverse-aggregation-landscapes-for-three-igg1-molecules
#14
Leon F Willis, Amit Kumar, John Dobson, Nick Bond, David Lowe, Richard Turner, Sheena E Radford, Nikil Kapur, David J Brockwell
Monoclonal antibodies (mAbs) currently dominate the biopharmaceutical sector due to their potency and efficacy against a range of disease targets. These proteinaceous therapeutics are, however, susceptible to unfolding, mis-folding and aggregation by environmental perturbations. Aggregation thus poses an enormous challenge to biopharmaceutical development, production, formulation and storage. Hydrodynamic forces have also been linked to aggregation, but the ability of different flow fields (e.g. shear and extensional flow) to trigger aggregation has remained unclear...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29313208/perspective-on-immune-oncology-with-liquid-biopsy-peripheral-blood-mononuclear-cells-and-microbiome-with-non-invasive-biomarkers-in-cancer-patients
#15
REVIEW
A Mitsuhashi, Y Okuma
Antibodies against immune checkpoint inhibitors such as anti-programmed cell death protein 1 (PD-1) and anti-programmed death ligand 1 (PD-L1) play a key role in the treatment of advanced lung cancer. To examine the clinical benefits of these agents, preclinical and clinical studies have been conducted to identify definitive biomarkers associated with cancer status. Analysis of the blood and feces of tumor patients has attracted attention in recent studies attempting to identify non-invasive biomarkers such as cytokines, soluble PD-L1, peripheral blood mononuclear cells, and gut microbiota...
January 8, 2018: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/29306502/identification-and-characterization-of-a-novel-protective-antigen-sec_205-of-streptococcus-equi-ssp-zooepidemicus
#16
Huihuang Liang, Bin Tang, Pengpeng Zhao, Mingyong Deng, Lili Yan, Pan Zhai, Zigong Wei
Streptococcus equi ssp. zooepidemicus (SEZ) is an important pathogen of swine streptococcal diseases and can infect a wide range of animals as well as human beings. The absence of effective vaccine confounds the control of SEZ infection. Sec_205, a novel protein identified in the previous study, was inducibly over-expressed in Escherichia coli in the present study. The purified recombinant protein could elicit a significant humoral antibody response and provide efficient protection against lethal challenge of SEZ C55138 in mouse model...
January 3, 2018: Vaccine
https://www.readbyqxmd.com/read/29306028/unique-mtb-protein-found-in-urine-of-tb-patients-discovery-of-a-unique-mycobacterium-tuberculosis-protein-through-proteomic-analysis-of-urine-from-patients-with-active-tuberculosis
#17
Nira Pollock, Rakesh Dhiman, Nada Daifalla, Maha Farhat, Antonio Campos-Neto
Identification of pathogen-specific biomarkers present in patients' serum or urine samples can be a useful diagnostic approach. In efforts to discover Mycobacterium tuberculosis (Mtb) biomarkers we identified by mass spectroscopy a unique 21-mer Mtb peptide sequence (VVLGLTVPGGVELLPGVALPR) present in the urines of TB patients from Zimbabwe. This peptide has 100% sequence homology with the protein TBCG_03312 from the C strain of Mtb (a clinical isolate identified in New York, NY, USA) and 95% sequence homology with Mtb oxidoreductase (MRGA423_21210) from the clinical isolate MTB423 (identified in Kerala, India)...
January 3, 2018: Microbes and Infection
https://www.readbyqxmd.com/read/29305849/prognosis-of-patients-with-stages-i-and-ii-non-small-cell-lung-cancer-with-nodal-micrometastases
#18
Paweł Gwóźdź, Monika Pasieka-Lis, Katarzyna Kołodziej, Juliusz Pankowski, Renata Banaś, Michał Wiłkojć, Marcin Zieliński
BACKGROUND: This retrospective study aimed to determine the prognostic significance of immunohistochemically detected occult micrometastases (OM) in mediastinal lymph nodes (LN) obtained during Transcervical Extended Mediastinal Lymphadenectomy (TEMLA) of stages I and II non-small cell small cancer (NSCLC) patients before complete surgical resection. METHODS: From January 2007 to June 2011, 75 patients with pathological stage I NSCLC and 73 patients with pathological stage II NSCLC underwent TEMLA and subsequent radical pulmonary resection...
January 3, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29304128/identification-of-a-protective-b-cell-epitope-of-the-staphylococcus-aureus-gapc-protein-by-screening-a-phage-displayed-random-peptide-library
#19
Mengyao Wang, Lu Zhai, Wei Yu, Yuhua Wei, Lizi Wang, Shuo Liu, Wanyu Li, Xiaoting Li, Simiao Yu, Xiaoting Chen, Hua Zhang, Jing Chen, Zhenyue Feng, Liquan Yu, Yudong Cui
The impact of epidemic Staphylococcus aureus (S. aureus) on public health is increasing. Because of the abuse of antibiotics, the antibiotic resistance of S. aureus is increasing. Thus, there is an urgent need to develop new immunotherapies and immunoprophylaxes. Previous studies showed that the GapC protein of S. aureus, which is a surface protein with high glyceraldehyde 3-phosphate dehydrogenase activity, transferrin binding activity, and other biological activities, is highly conserved. GapC induces an effective humoral immune response in vivo...
2018: PloS One
https://www.readbyqxmd.com/read/29303554/a-serological-point-of-care-test-for-the-detection-of-igg-antibodies-against-ebola-virus-in-human-survivors
#20
Polina Brangel, Ariel Sobarzo, Claudio Parolo, Benjamin S Miller, Philip D Howes, Sigal Gelkop, Julius J Lutwama, John M Dye, Rachel A McKendry, Leslie Lobel, Molly M Stevens
Ebola virus disease causes widespread and highly fatal epidemics in human populations. Today, there is still great need for point-of-care tests for diagnosis, patient management and surveillance, both during and post outbreaks. We present a point-of-care test comprising an immunochromatographic strip and a smartphone reader, which detects and semiquantifies Ebola-specific antibodies in human survivors. We developed a Sudan virus glycoprotein monoplex platform and validated it using sera from 90 human survivors and 31 local noninfected controls...
January 5, 2018: ACS Nano
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