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vascular smooth muscle cell lineage tracing

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https://www.readbyqxmd.com/read/28473437/endothelial-myocyte-enhancer-factor-2c-inhibits-migration-of-smooth-muscle-cells-through-fenestrations-in-the-internal-elastic-lamina
#1
Yao Wei Lu, Anthony M Lowery, Li-Yan Sun, Harold A Singer, Guohao Dai, Alejandro P Adam, Peter A Vincent, John J Schwarz
OBJECTIVE: Laminar flow activates myocyte enhancer factor 2 (MEF2) transcription factors in vitro to induce expression of atheroprotective genes in the endothelium. Here we sought to establish the role of Mef2c in the vascular endothelium in vivo. APPROACH AND RESULTS: To study endothelial Mef2c, we generated endothelial-specific deletion of Mef2c using Tie2-Cre or Cdh5-Cre-ER(T2) and examined aortas and carotid arteries by en face immunofluorescence. We observed enhanced actin stress fiber formation in the Mef2c-deleted thoracic aortic endothelium (laminar flow region), similar to those observed in normal aortic inner curvature (disturbed flow region)...
May 4, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28463231/disruption-of-lineage-specification-in-adult-pulmonary-mesenchymal-progenitor-cells-promotes-microvascular-dysfunction
#2
Christa F Gaskill, Erica J Carrier, Jonathan A Kropski, Nathaniel C Bloodworth, Swapna Menon, Robert F Foronjy, M Mark Taketo, Charles C Hong, Eric D Austin, James D West, Anna L Means, James E Loyd, W David Merryman, Anna R Hemnes, Stijn De Langhe, Timothy S Blackwell, Dwight J Klemm, Susan M Majka
Pulmonary vascular disease is characterized by remodeling and loss of microvessels and is typically attributed to pathological responses in vascular endothelium or abnormal smooth muscle cell phenotypes. We have challenged this understanding by defining an adult pulmonary mesenchymal progenitor cell (MPC) that regulates both microvascular function and angiogenesis. The current understanding of adult MPCs and their roles in homeostasis versus disease has been limited by a lack of genetic markers with which to lineage label multipotent mesenchyme and trace the differentiation of these MPCs into vascular lineages...
May 2, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28370670/origin-and-characterization-of-alpha-smooth-muscle-actin-positive-cells-during-murine-lung-development
#3
Alena Moiseenko, Vahid Kheirollahi, Cho-Ming Chao, Negah Ahmadvand, Jennifer Quantius, Jochen Wilhelm, Susanne Herold, Katrin Ahlbrecht, Rory E Morty, Albert A Rizvanov, Parviz Minoo, Elie El Agha, Saverio Bellusci
ACTA2 expression identifies pulmonary airway and vascular smooth muscle cells (SMCs) as well as alveolar myofibroblasts (MYF). Mesenchymal progenitors expressing fibroblast growth factor 10 (Fgf10), Wilms tumor 1 (Wt1), or glioma-associated oncogene 1 (Gli1) contribute to SMC formation from early stages of lung development. However, their respective contribution and specificity to the SMC and/or alveolar MYF lineages remain controversial. In addition, the contribution of mesenchymal cells undergoing active WNT signaling remains unknown...
April 3, 2017: Stem Cells
https://www.readbyqxmd.com/read/28360226/dual-function-for-mature-vascular-smooth-muscle-cells-during-arteriovenous-fistula-remodeling
#4
Jinjing Zhao, Frances L Jourd'heuil, Min Xue, David Conti, Reynold I Lopez-Soler, Roman Ginnan, Arif Asif, Harold A Singer, David Jourd'heuil, Xiaochun Long
BACKGROUND: The arteriovenous fistula (AVF) is the preferred form of hemodialysis access for patients with chronic kidney disease. However, AVFs are associated with significant problems including high incidence of both early and late failures, usually attributed to inadequate venous arterialization and neointimal hyperplasia, respectively. Understanding the cellular basis of venous remodeling in the setting of AVF could provide targets for improving AVF patency rates. METHODS AND RESULTS: A novel vascular smooth muscle cell (VSMC) lineage tracing reporter mouse, Myh11-Cre/ERT2-mTmG, was used to track mature VSMCs in a clinically relevant AVF mouse model created by a jugular vein branch end to carotid artery side anastomosis...
March 30, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28111199/pericytes-of-multiple-organs-do-not-behave-as-mesenchymal-stem-cells-in%C3%A2-vivo
#5
Nuno Guimarães-Camboa, Paola Cattaneo, Yunfu Sun, Thomas Moore-Morris, Yusu Gu, Nancy D Dalton, Edward Rockenstein, Eliezer Masliah, Kirk L Peterson, William B Stallcup, Ju Chen, Sylvia M Evans
Pericytes are widely believed to function as mesenchymal stem cells (MSCs), multipotent tissue-resident progenitors with great potential for regenerative medicine. Cultured pericytes isolated from distinct tissues can differentiate into multiple cell types in vitro or following transplantation in vivo. However, the cell fate plasticity of endogenous pericytes in vivo remains unclear. Here, we show that the transcription factor Tbx18 selectively marks pericytes and vascular smooth muscle cells in multiple organs of adult mouse...
March 2, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28060852/contribution-of-vascular-cells-to-neointimal-formation
#6
Falei Yuan, Dong Wang, Kang Xu, Jixian Wang, Zhijun Zhang, Li Yang, Guo-Yuan Yang, Song Li
The de-differentiation and proliferation of smooth muscle cells (SMCs) are widely accepted as the major contributor to vascular remodeling. However, recent studies indicate that vascular stem cells (VSCs) also play an important role, but their relative contribution remains to be elucidated. In this study, we used genetic lineage tracing approach to further investigate the contribution of SMCs and VSCs to neointimal thickening in response to endothelium denudation injury or artery ligation. In vitro and in vivo analysis of MYH11-cre/Rosa-loxP-RFP mouse artery showed that SMCs proliferated at a much slower rate than non-SMCs...
2017: PloS One
https://www.readbyqxmd.com/read/27682618/extensive-proliferation-of-a-subset-of-differentiated-yet-plastic-medial-vascular-smooth-muscle-cells-contributes-to-neointimal-formation-in-mouse-injury-and-atherosclerosis-models
#7
Joel Chappell, Jennifer L Harman, Vagheesh M Narasimhan, Haixiang Yu, Kirsty Foote, Benjamin D Simons, Martin R Bennett, Helle F Jørgensen
RATIONALE: Vascular smooth muscle cell (VSMC) accumulation is a hallmark of atherosclerosis and vascular injury. However, fundamental aspects of proliferation and the phenotypic changes within individual VSMCs, which underlie vascular disease, remain unresolved. In particular, it is not known whether all VSMCs proliferate and display plasticity or whether individual cells can switch to multiple phenotypes. OBJECTIVE: To assess whether proliferation and plasticity in disease is a general characteristic of VSMCs or a feature of a subset of cells...
December 9, 2016: Circulation Research
https://www.readbyqxmd.com/read/27403660/characterisation-of-cultured-mesothelial-cells-derived-from-the-murine-adult-omentum
#8
Sumaya Dauleh, Ilaria Santeramo, Claire Fielding, Kelly Ward, Anne Herrmann, Patricia Murray, Bettina Wilm
The human omentum has been long regarded as a healing patch, used by surgeons for its ability to immunomodulate, repair and vascularise injured tissues. A major component of the omentum are mesothelial cells, which display some of the characteristics of mesenchymal stem/stromal cells. For instance, lineage tracing studies have shown that mesothelial cells give rise to adipocytes and vascular smooth muscle cells, and human and rat mesothelial cells have been shown to differentiate into osteoblast- and adipocyte-like cells in vitro, indicating that they have considerable plasticity...
2016: PloS One
https://www.readbyqxmd.com/read/27320174/lineage-tracing-of-cells-involved-in-atherosclerosis
#9
Julián Albarrán-Juárez, Harmandeep Kaur, Myriam Grimm, Stefan Offermanns, Nina Wettschureck
BACKGROUND AND AIMS: Despite the clinical importance of atherosclerosis, the origin of cells within atherosclerotic plaques is not fully understood. Due to the lack of a definitive lineage-tracing strategy, previous studies have provided controversial results about the origin of cells expressing smooth muscle and macrophage markers in atherosclerosis. We here aim to identify the origin of vascular smooth muscle (SM) cells and macrophages within atherosclerosis lesions. METHODS: We combined a genetic fate mapping approach with single cell expression analysis in a murine model of atherosclerosis...
August 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27060598/pdgfrb-cre-targets-lymphatic-endothelial-cells-of-both-venous-and-non-venous-origins
#10
Maria H Ulvmar, Ines Martinez-Corral, Lukas Stanczuk, Taija Mäkinen
The Pdgfrb-Cre line has been used as a tool to specifically target pericytes and vascular smooth muscle cells. Recent studies showed additional targeting of cardiac and mesenteric lymphatic endothelial cells (LECs) by the Pdgfrb-Cre transgene. In the heart, this was suggested to provide evidence for a previously unknown nonvenous source of LECs originating from yolk sac (YS) hemogenic endothelium (HemEC). Here we show that Pdgfrb-Cre does not, however, target YS HemEC or YS-derived erythro-myeloid progenitors (EMPs)...
2016: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/26952986/clarification-of-mural-cell-coverage-of-vascular-endothelial-cells-by-live-imaging-of-zebrafish
#11
Koji Ando, Shigetomo Fukuhara, Nanae Izumi, Hiroyuki Nakajima, Hajime Fukui, Robert N Kelsh, Naoki Mochizuki
Mural cells (MCs) consisting of vascular smooth muscle cells and pericytes cover the endothelial cells (ECs) to regulate vascular stability and homeostasis. Here, we clarified the mechanism by which MCs develop and cover ECs by generating transgenic zebrafish lines that allow live imaging of MCs and by lineage tracing in vivo To cover cranial vessels, MCs derived from either neural crest cells or mesoderm emerged around the preformed EC tubes, proliferated and migrated along EC tubes. During their migration, the MCs moved forward by extending their processes along the inter-EC junctions, suggesting a role for inter-EC junctions as a scaffold for MC migration...
April 15, 2016: Development
https://www.readbyqxmd.com/read/26902114/smooth-muscle-origin-of-postnatal-2nd-cvp-is-pre-determined-in-early-embryo
#12
Qiaozhen Liu, Hui Zhang, Xueying Tian, Lingjuan He, Xiuzhen Huang, Zhen Tan, Yan Yan, Sylvia M Evans, Joshua D Wythe, Bin Zhou
Recent identification of the neonatal 2nd coronary vascular population (2nd CVP) suggests that a subset of these vessels form de novo and mature in the inner myocardial wall of the postnatal heart. However, the origin of smooth muscle cells (SMCs) in the postnatal 2nd CVP remains undetermined. Using a tamoxifen-inducible Wt1-CreER driver and a Rosa26-RFP reporter line, we traced the lineage of epicardial cells to determine if they contribute to SMCs of the 2nd CVP. Late embryonic and postnatal induction of Wt1-CreER activity demonstrated that at these stages Wt1-labeled epicardium does not significantly migrate into the myocardium to form SMCs...
March 18, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26892967/vascular-smooth-muscle-cells-in-atherosclerosis
#13
REVIEW
Martin R Bennett, Sanjay Sinha, Gary K Owens
The historical view of vascular smooth muscle cells (VSMCs) in atherosclerosis is that aberrant proliferation of VSMCs promotes plaque formation, but that VSMCs in advanced plaques are entirely beneficial, for example preventing rupture of the fibrous cap. However, this view has been based on ideas that there is a homogenous population of VSMCs within the plaque, that can be identified separate from other plaque cells (particularly macrophages) using standard VSMC and macrophage immunohistochemical markers...
February 19, 2016: Circulation Research
https://www.readbyqxmd.com/read/26693821/origin-of-matrix-producing-cells-that-contribute-to-aortic-fibrosis-in-hypertension
#14
Jing Wu, Kim Ramil C Montaniel, Mohamed A Saleh, Liang Xiao, Wei Chen, Gary K Owens, Jay D Humphrey, Mark W Majesky, David T Paik, Antonis K Hatzopoulos, Meena S Madhur, David G Harrison
Various hypertensive stimuli lead to exuberant adventitial collagen deposition in large arteries, exacerbating blood pressure elevation and end-organ damage. Collagen production is generally attributed to resident fibroblasts; however, other cells, including resident and bone marrow-derived stem cell antigen positive (Sca-1(+)) cells and endothelial and vascular smooth muscle cells, can produce collagen and contribute to vascular stiffening. Using flow cytometry and immunofluorescence, we found that adventitial Sca-1(+) progenitor cells begin to produce collagen and acquire a fibroblast-like phenotype in hypertension...
February 2016: Hypertension
https://www.readbyqxmd.com/read/26650841/kinetics-of-label-retaining-cells-in-the-developing-rat-kidneys
#15
Jianwen Wang, Guiting Lin, Amjad Alwaal, Xiaoyu Zhang, Guifang Wang, Xingyuan Jia, Lia Banie, Jacqueline Villalta, Ching-Shwun Lin, Tom F Lue
BACKGROUND: The kidney is a specialized low-regenerative organ with several different types of cellular lineages. The BrdU label-retaining cell (LRCs) approach has been used as part of a strategy to identify tissue-specific stem cells in the kidney; however, because the complementary base pairing in double-stranded DNA blocks the access of the anti-BrdU antibody to BrdU subunits, the stem cell marker expression in BrdU-labeled cells are often difficult to detect. In this study, we introduced a new cell labeling and detection method in which BrdU was replaced with 5-ethynyl-2-deoxyuridine (EdU) and examined the time-dependent dynamic changes of EdU-labeled cells and potential stem/progenitor markers in the development of kidney...
2015: PloS One
https://www.readbyqxmd.com/read/26573225/abcg2-labeled-cells-contribute-to-different-cell-populations-in-the-embryonic-and-adult-heart
#16
Michelle J Doyle, Travis J Maher, Qinglu Li, Mary G Garry, Brian P Sorrentino, Cindy M Martin
ATP-binding cassette transporter subfamily G member 2 (Abcg2)-expressing cardiac-side population cells have been identified in the developing and adult heart, although the role they play in mammalian heart growth and regeneration remains unclear. In this study, we use genetic lineage tracing to follow the cell fate of Abcg2-expressing cells in the embryonic and adult heart. During cardiac embryogenesis, the Abcg2 lineage gives rise to multiple cardiovascular cell types, including cardiomyocytes, endothelial cells, and vascular smooth muscle cells...
February 1, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/26020946/coronary-artery-disease-associated-transcription-factor-tcf21-regulates-smooth-muscle-precursor-cells-that-contribute-to-the-fibrous-cap
#17
Sylvia T Nurnberg, Karen Cheng, Azad Raiesdana, Ramendra Kundu, Clint L Miller, Juyong B Kim, Komal Arora, Ivan Carcamo-Oribe, Yiqin Xiong, Nikhil Tellakula, Vivek Nanda, Nikitha Murthy, William A Boisvert, Ulf Hedin, Ljubica Perisic, Silvia Aldi, Lars Maegdefessel, Milos Pjanic, Gary K Owens, Michelle D Tallquist, Thomas Quertermous
Recent genome wide association studies have identified a number of genes that contribute to the risk for coronary heart disease. One such gene, TCF21, encodes a basic-helix-loop-helix transcription factor believed to serve a critical role in the development of epicardial progenitor cells that give rise to coronary artery smooth muscle cells (SMC) and cardiac fibroblasts. Using reporter gene and immunolocalization studies with mouse and human tissues we have found that vascular TCF21 expression in the adult is restricted primarily to adventitial cells associated with coronary arteries and also medial SMC in the proximal aorta of mouse...
May 2015: PLoS Genetics
https://www.readbyqxmd.com/read/25834185/endothelial-plasticity-drives-arterial-remodeling-within-the-endocardium-after-myocardial-infarction
#18
Lucile Miquerol, Jérome Thireau, Patrice Bideaux, Rachel Sturny, Sylvain Richard, Robert G Kelly
RATIONALE: Revascularization of injured, ischemic, and regenerating organs is essential to restore organ function. In the postinfarct heart, however, the mechanisms underlying the formation of new coronary arteries are poorly understood. OBJECTIVE: To study vascular remodeling of coronary arteries after infarction. METHODS AND RESULTS: We performed permanent left coronary ligation on Connexin40-GFP mice expressing green fluorescent protein (GFP) in endothelial cells of coronary arteries but not veins, capillaries, or endocardium...
May 22, 2015: Circulation Research
https://www.readbyqxmd.com/read/25634211/tumor-endothelial-cells-with-distinct-patterns-of-tgf%C3%AE-driven-endothelial-to-mesenchymal-transition
#19
Lin Xiao, Dae Joong Kim, Clayton L Davis, James V McCann, James M Dunleavey, Alissa K Vanderlinden, Nuo Xu, Samantha G Pattenden, Stephen V Frye, Xia Xu, Mark Onaitis, Elizabeth Monaghan-Benson, Keith Burridge, Andrew C Dudley
Endothelial-to-mesenchymal transition (EndMT) occurs during development and underlies the pathophysiology of multiple diseases. In tumors, unscheduled EndMT generates cancer-associated myofibroblasts that fuel inflammation and fibrosis, and may contribute to vascular dysfunction that promotes tumor progression. We report that freshly isolated subpopulations of tumor-specific endothelial cells (TEC) from a spontaneous mammary tumor model undergo distinct forms of EndMT in response to TGFβ stimulation. Although some TECs strikingly upregulate α smooth muscle actin (SMA), a principal marker of EndMT and activated myofibroblasts, counterpart normal mammary gland endothelial cells (NEC) showed little change in SMA expression after TGFβ treatment...
April 1, 2015: Cancer Research
https://www.readbyqxmd.com/read/25064100/genetic-inducible-fate-mapping-in-adult-mice-using-tamoxifen-dependent-cre-recombinases
#20
Susanne Feil, Jana Krauss, Martin Thunemann, Robert Feil
The Cre/lox site-specific recombination system allows the control of gene activity in space and time in almost any tissue of the mouse. A major technical advance was the development of tamoxifen-dependent Cre recombinases, such as CreER(T2), that can be activated by administration of tamoxifen to the animal. This powerful tool greatly facilitates the study of gene functions and the generation of more realistic animal models of sporadic human diseases. Another important application of tamoxifen-dependent Cre recombinases is genetic inducible fate mapping (GIFM)...
2014: Methods in Molecular Biology
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