keyword
MENU ▼
Read by QxMD icon Read
search

vascular smooth muscle cell lineage tracing

keyword
https://www.readbyqxmd.com/read/27682618/extensive-proliferation-of-a-subset-of-differentiated-yet-plastic-medial-vascular-smooth-muscle-cells-contribute-to-neointimal-formation-in-mouse-injury-and-atherosclerosis-models
#1
Joel Chappell, Jennifer L Harman, Vagheesh M Narasimhan, Haixiang Yu, Kirsty Foote, Benjamin D Simons, Martin R Bennett, Helle F Jorgensen
RATIONALE: Vascular smooth muscle cell (VSMC) accumulation is a hallmark of atherosclerosis and vascular injury. However, fundamental aspects of proliferation and the phenotypic changes within individual VSMCs, which underlie vascular disease remain unresolved. In particular, it is not known if all VSMCs proliferate and display plasticity, or whether individual cells can switch to multiple phenotypes. OBJECTIVE: To assess whether proliferation and plasticity in disease is a general characteristic of VSMCs or a feature of a subset of cells...
September 28, 2016: Circulation Research
https://www.readbyqxmd.com/read/27403660/characterisation-of-cultured-mesothelial-cells-derived-from-the-murine-adult-omentum
#2
Sumaya Dauleh, Ilaria Santeramo, Claire Fielding, Kelly Ward, Anne Herrmann, Patricia Murray, Bettina Wilm
The human omentum has been long regarded as a healing patch, used by surgeons for its ability to immunomodulate, repair and vascularise injured tissues. A major component of the omentum are mesothelial cells, which display some of the characteristics of mesenchymal stem/stromal cells. For instance, lineage tracing studies have shown that mesothelial cells give rise to adipocytes and vascular smooth muscle cells, and human and rat mesothelial cells have been shown to differentiate into osteoblast- and adipocyte-like cells in vitro, indicating that they have considerable plasticity...
2016: PloS One
https://www.readbyqxmd.com/read/27320174/lineage-tracing-of-cells-involved-in-atherosclerosis
#3
Julián Albarrán-Juárez, Harmandeep Kaur, Myriam Grimm, Stefan Offermanns, Nina Wettschureck
BACKGROUND AND AIMS: Despite the clinical importance of atherosclerosis, the origin of cells within atherosclerotic plaques is not fully understood. Due to the lack of a definitive lineage-tracing strategy, previous studies have provided controversial results about the origin of cells expressing smooth muscle and macrophage markers in atherosclerosis. We here aim to identify the origin of vascular smooth muscle (SM) cells and macrophages within atherosclerosis lesions. METHODS: We combined a genetic fate mapping approach with single cell expression analysis in a murine model of atherosclerosis...
August 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27060598/pdgfrb-cre-targets-lymphatic-endothelial-cells-of-both-venous-and-non-venous-origins
#4
Maria H Ulvmar, Ines Martinez-Corral, Lukas Stanczuk, Taija Mäkinen
The Pdgfrb-Cre line has been used as a tool to specifically target pericytes and vascular smooth muscle cells. Recent studies showed additional targeting of cardiac and mesenteric lymphatic endothelial cells (LECs) by the Pdgfrb-Cre transgene. In the heart, this was suggested to provide evidence for a previously unknown nonvenous source of LECs originating from yolk sac (YS) hemogenic endothelium (HemEC). Here we show that Pdgfrb-Cre does not, however, target YS HemEC or YS-derived erythro-myeloid progenitors (EMPs)...
June 2016: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/26952986/clarification-of-mural-cell-coverage-of-vascular-endothelial-cells-by-live-imaging-of-zebrafish
#5
Koji Ando, Shigetomo Fukuhara, Nanae Izumi, Hiroyuki Nakajima, Hajime Fukui, Robert N Kelsh, Naoki Mochizuki
Mural cells (MCs) consisting of vascular smooth muscle cells and pericytes cover the endothelial cells (ECs) to regulate vascular stability and homeostasis. Here, we clarified the mechanism by which MCs develop and cover ECs by generating transgenic zebrafish lines that allow live imaging of MCs and by lineage tracing in vivo To cover cranial vessels, MCs derived from either neural crest cells or mesoderm emerged around the preformed EC tubes, proliferated and migrated along EC tubes. During their migration, the MCs moved forward by extending their processes along the inter-EC junctions, suggesting a role for inter-EC junctions as a scaffold for MC migration...
April 15, 2016: Development
https://www.readbyqxmd.com/read/26902114/smooth-muscle-origin-of-postnatal-2nd-cvp-is-pre-determined-in-early-embryo
#6
Qiaozhen Liu, Hui Zhang, Xueying Tian, Lingjuan He, Xiuzhen Huang, Zhen Tan, Yan Yan, Sylvia M Evans, Joshua D Wythe, Bin Zhou
Recent identification of the neonatal 2nd coronary vascular population (2nd CVP) suggests that a subset of these vessels form de novo and mature in the inner myocardial wall of the postnatal heart. However, the origin of smooth muscle cells (SMCs) in the postnatal 2nd CVP remains undetermined. Using a tamoxifen-inducible Wt1-CreER driver and a Rosa26-RFP reporter line, we traced the lineage of epicardial cells to determine if they contribute to SMCs of the 2nd CVP. Late embryonic and postnatal induction of Wt1-CreER activity demonstrated that at these stages Wt1-labeled epicardium does not significantly migrate into the myocardium to form SMCs...
March 18, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26892967/vascular-smooth-muscle-cells-in-atherosclerosis
#7
REVIEW
Martin R Bennett, Sanjay Sinha, Gary K Owens
The historical view of vascular smooth muscle cells (VSMCs) in atherosclerosis is that aberrant proliferation of VSMCs promotes plaque formation, but that VSMCs in advanced plaques are entirely beneficial, for example preventing rupture of the fibrous cap. However, this view has been based on ideas that there is a homogenous population of VSMCs within the plaque, that can be identified separate from other plaque cells (particularly macrophages) using standard VSMC and macrophage immunohistochemical markers...
February 19, 2016: Circulation Research
https://www.readbyqxmd.com/read/26693821/origin-of-matrix-producing-cells-that-contribute-to-aortic-fibrosis-in-hypertension
#8
Jing Wu, Kim Ramil C Montaniel, Mohamed A Saleh, Liang Xiao, Wei Chen, Gary K Owens, Jay D Humphrey, Mark W Majesky, David T Paik, Antonis K Hatzopoulos, Meena S Madhur, David G Harrison
Various hypertensive stimuli lead to exuberant adventitial collagen deposition in large arteries, exacerbating blood pressure elevation and end-organ damage. Collagen production is generally attributed to resident fibroblasts; however, other cells, including resident and bone marrow-derived stem cell antigen positive (Sca-1(+)) cells and endothelial and vascular smooth muscle cells, can produce collagen and contribute to vascular stiffening. Using flow cytometry and immunofluorescence, we found that adventitial Sca-1(+) progenitor cells begin to produce collagen and acquire a fibroblast-like phenotype in hypertension...
February 2016: Hypertension
https://www.readbyqxmd.com/read/26650841/kinetics-of-label-retaining-cells-in-the-developing-rat-kidneys
#9
Jianwen Wang, Guiting Lin, Amjad Alwaal, Xiaoyu Zhang, Guifang Wang, Xingyuan Jia, Lia Banie, Jacqueline Villalta, Ching-Shwun Lin, Tom F Lue
BACKGROUND: The kidney is a specialized low-regenerative organ with several different types of cellular lineages. The BrdU label-retaining cell (LRCs) approach has been used as part of a strategy to identify tissue-specific stem cells in the kidney; however, because the complementary base pairing in double-stranded DNA blocks the access of the anti-BrdU antibody to BrdU subunits, the stem cell marker expression in BrdU-labeled cells are often difficult to detect. In this study, we introduced a new cell labeling and detection method in which BrdU was replaced with 5-ethynyl-2-deoxyuridine (EdU) and examined the time-dependent dynamic changes of EdU-labeled cells and potential stem/progenitor markers in the development of kidney...
2015: PloS One
https://www.readbyqxmd.com/read/26573225/abcg2-labeled-cells-contribute-to-different-cell-populations-in-the-embryonic-and-adult-heart
#10
Michelle J Doyle, Travis J Maher, Qinglu Li, Mary G Garry, Brian P Sorrentino, Cindy M Martin
ATP-binding cassette transporter subfamily G member 2 (Abcg2)-expressing cardiac-side population cells have been identified in the developing and adult heart, although the role they play in mammalian heart growth and regeneration remains unclear. In this study, we use genetic lineage tracing to follow the cell fate of Abcg2-expressing cells in the embryonic and adult heart. During cardiac embryogenesis, the Abcg2 lineage gives rise to multiple cardiovascular cell types, including cardiomyocytes, endothelial cells, and vascular smooth muscle cells...
February 1, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/26020946/coronary-artery-disease-associated-transcription-factor-tcf21-regulates-smooth-muscle-precursor-cells-that-contribute-to-the-fibrous-cap
#11
Sylvia T Nurnberg, Karen Cheng, Azad Raiesdana, Ramendra Kundu, Clint L Miller, Juyong B Kim, Komal Arora, Ivan Carcamo-Oribe, Yiqin Xiong, Nikhil Tellakula, Vivek Nanda, Nikitha Murthy, William A Boisvert, Ulf Hedin, Ljubica Perisic, Silvia Aldi, Lars Maegdefessel, Milos Pjanic, Gary K Owens, Michelle D Tallquist, Thomas Quertermous
Recent genome wide association studies have identified a number of genes that contribute to the risk for coronary heart disease. One such gene, TCF21, encodes a basic-helix-loop-helix transcription factor believed to serve a critical role in the development of epicardial progenitor cells that give rise to coronary artery smooth muscle cells (SMC) and cardiac fibroblasts. Using reporter gene and immunolocalization studies with mouse and human tissues we have found that vascular TCF21 expression in the adult is restricted primarily to adventitial cells associated with coronary arteries and also medial SMC in the proximal aorta of mouse...
May 2015: PLoS Genetics
https://www.readbyqxmd.com/read/25834185/endothelial-plasticity-drives-arterial-remodeling-within-the-endocardium-after-myocardial-infarction
#12
Lucile Miquerol, Jérome Thireau, Patrice Bideaux, Rachel Sturny, Sylvain Richard, Robert G Kelly
RATIONALE: Revascularization of injured, ischemic, and regenerating organs is essential to restore organ function. In the postinfarct heart, however, the mechanisms underlying the formation of new coronary arteries are poorly understood. OBJECTIVE: To study vascular remodeling of coronary arteries after infarction. METHODS AND RESULTS: We performed permanent left coronary ligation on Connexin40-GFP mice expressing green fluorescent protein (GFP) in endothelial cells of coronary arteries but not veins, capillaries, or endocardium...
May 22, 2015: Circulation Research
https://www.readbyqxmd.com/read/25634211/tumor-endothelial-cells-with-distinct-patterns-of-tgf%C3%AE-driven-endothelial-to-mesenchymal-transition
#13
Lin Xiao, Dae Joong Kim, Clayton L Davis, James V McCann, James M Dunleavey, Alissa K Vanderlinden, Nuo Xu, Samantha G Pattenden, Stephen V Frye, Xia Xu, Mark Onaitis, Elizabeth Monaghan-Benson, Keith Burridge, Andrew C Dudley
Endothelial-to-mesenchymal transition (EndMT) occurs during development and underlies the pathophysiology of multiple diseases. In tumors, unscheduled EndMT generates cancer-associated myofibroblasts that fuel inflammation and fibrosis, and may contribute to vascular dysfunction that promotes tumor progression. We report that freshly isolated subpopulations of tumor-specific endothelial cells (TEC) from a spontaneous mammary tumor model undergo distinct forms of EndMT in response to TGFβ stimulation. Although some TECs strikingly upregulate α smooth muscle actin (SMA), a principal marker of EndMT and activated myofibroblasts, counterpart normal mammary gland endothelial cells (NEC) showed little change in SMA expression after TGFβ treatment...
April 1, 2015: Cancer Research
https://www.readbyqxmd.com/read/25064100/genetic-inducible-fate-mapping-in-adult-mice-using-tamoxifen-dependent-cre-recombinases
#14
Susanne Feil, Jana Krauss, Martin Thunemann, Robert Feil
The Cre/lox site-specific recombination system allows the control of gene activity in space and time in almost any tissue of the mouse. A major technical advance was the development of tamoxifen-dependent Cre recombinases, such as CreER(T2), that can be activated by administration of tamoxifen to the animal. This powerful tool greatly facilitates the study of gene functions and the generation of more realistic animal models of sporadic human diseases. Another important application of tamoxifen-dependent Cre recombinases is genetic inducible fate mapping (GIFM)...
2014: Methods in Molecular Biology
https://www.readbyqxmd.com/read/24727670/tbx18-is-essential-for-normal-development-of-vasculature-network-and-glomerular-mesangium-in-the-mammalian-kidney
#15
Jinshu Xu, Xuguang Nie, Xiaoqiang Cai, Chen-Leng Cai, Pin-Xian Xu
Tbx18 has been shown to be essential for ureteral development. However, it remains unclear whether it plays a direct role in kidney development. Here we addressed this by focusing on examining the pattern and contribution of Tbx18+ cells in the kidney and its role in kidney vascular development. Expression studies and genetic lineage tracing revealed that Tbx18 is expressed in renal capsule, vascular smooth muscle cells and pericytes and glomerular mesangial cells in the kidney and that Tbx18-expressing progenitors contribute to these cell types...
July 1, 2014: Developmental Biology
https://www.readbyqxmd.com/read/24353064/fgf10-positive-cells-represent-a-progenitor-cell-population-during-lung-development-and-postnatally
#16
Elie El Agha, Susanne Herold, Denise Al Alam, Jennifer Quantius, BreAnne MacKenzie, Gianni Carraro, Alena Moiseenko, Cho-Ming Chao, Parviz Minoo, Werner Seeger, Saverio Bellusci
The lung mesenchyme consists of a widely heterogeneous population of cells that play crucial roles during development and homeostasis after birth. These cells belong to myogenic, adipogenic, chondrogenic, neuronal and other lineages. Yet, no clear hierarchy for these lineages has been established. We have previously generated a novel Fgf10(iCre) knock-in mouse line that allows lineage tracing of Fgf10-positive cells during development and postnatally. Using these mice, we hereby demonstrate the presence of two waves of Fgf10 expression during embryonic lung development: the first wave, comprising Fgf10-positive cells residing in the submesothelial mesenchyme at early pseudoglandular stage (as well as their descendants); and the second wave, comprising Fgf10-positive cells from late pseudoglandular stage (as well as their descendants)...
January 2014: Development
https://www.readbyqxmd.com/read/24270161/smooth-muscle-cells-largely-develop-independently-of-functional-hemogenic-endothelium
#17
Monika Stefanska, Guilherme Costa, Michael Lie-A-Ling, Valerie Kouskoff, Georges Lacaud
Vascular smooth muscle cells represent a major component of the cardiovascular system. In vitro studies have shown that FLK1(+) cells derived from embryonic stem (ES) cells can differentiate into both endothelial and smooth muscle cells. These FLK1(+) cells also contain a mesodermal precursor, the hemangioblast, able to produce endothelial, blood and smooth muscle cells. The generation of blood precursors from the hemangioblast was recently shown to occur through a transient cell population of specialised endothelium, a hemogenic endothelium...
January 2014: Stem Cell Research
https://www.readbyqxmd.com/read/24130328/derivation-of-lung-mesenchymal-lineages-from-the-fetal-mesothelium-requires-hedgehog-signaling-for-mesothelial-cell-entry
#18
Radhika Dixit, Xingbin Ai, Alan Fine
Recent studies have shown that mesothelial progenitors contribute to mesenchymal lineages of developing organs. To what extent the overlying mesothelium contributes to lung development remains unknown. To rigorously address this question, we employed Wt1(CreERT2/+) mice for high-fidelity lineage tracing after confirming that Cre recombinase was mesothelial specific and faithfully recapitulated endogenous Wilms' tumor 1 (Wt1) gene expression. We visualized WT1(+) mesothelial cell entry into the lung by live imaging and identified their progenies in subpopulations of bronchial smooth muscle cells, vascular smooth muscle cells and desmin(+) fibroblasts by lineage tagging...
November 2013: Development
https://www.readbyqxmd.com/read/23873040/coordination-of-heart-and-lung-co-development-by-a-multipotent-cardiopulmonary-progenitor
#19
Tien Peng, Ying Tian, Cornelis J Boogerd, Min Min Lu, Rachel S Kadzik, Kathleen M Stewart, Sylvia M Evans, Edward E Morrisey
Co-development of the cardiovascular and pulmonary systems is a recent evolutionary adaption to terrestrial life that couples cardiac output with the gas exchange function of the lung. Here we show that the murine pulmonary vasculature develops even in the absence of lung development. We have identified a population of multipotent cardiopulmonary mesoderm progenitors (CPPs) within the posterior pole of the heart that are marked by the expression of Wnt2, Gli1 and Isl1. We show that CPPs arise from cardiac progenitors before lung development...
August 29, 2013: Nature
https://www.readbyqxmd.com/read/23662173/dysfunctional-resident-lung-mesenchymal-stem-cells-contribute-to-pulmonary-microvascular-remodeling
#20
Kelsey Chow, Joshua P Fessel, Kaoriihida-Stansbury, Eric P Schmidt, Christa Gaskill, Diego Alvarez, Brian Graham, David G Harrison, David H Wagner, Eva Nozik-Grayck, James D West, Dwight J Klemm, Susan M Majka
Pulmonary vascular remodeling and oxidative stress are common to many adult lung diseases. However, little is known about the relevance of lung mesenchymal stem cells (MSCs) in these processes. We tested the hypothesis that dysfunctional lung MSCs directly participate in remodeling of the microcirculation. We employed a genetic model to deplete extracellular superoxide dismutase (EC-SOD) in lung MSCs coupled with lineage tracing analysis. We crossed (floxp)sod3 and mT/mG reporter mice to a strain expressing Cre recombinase under the control of the ABCG2 promoter...
January 2013: Pulmonary Circulation
keyword
keyword
116631
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"