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carotid ligation mouse model

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https://www.readbyqxmd.com/read/29407891/fluid-shear-stress-regulates-the-expression-of-lectin-like-oxidized-low-density-lipoprotein-receptor-1-via-klf2-ap-1-pathway-depending-on-its-intensity-and-pattern-in-endothelial-cells
#1
Ji Yoon Lee, Jihwa Chung, Kyoung Hwa Kim, Shung Hyun An, Minsuk Kim, Junbeom Park, Kihwan Kwon
BACKGROUND AND AIMS: Vascular endothelial cells (ECs) are exposed to fluid shear stress (FSS), which modulates vascular pathophysiology. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is crucial in endothelial dysfunction and atherosclerosis. We elucidated the mechanism regulating LOX-1 expression in ECs by FSS. METHODS: Human umbilical vein endothelial cells were exposed to laminar shear stress (LSS) of indicated intensities using a unidirectional steady flow, or to oscillatory shear stress (OSS) using a bidirectional disturbed flow...
January 31, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29363221/pretreatment-with-magnesium-sulfate-attenuates-white-matter-damage-by-preventing-cell-death-of-developing-oligodendrocytes
#2
Takahiro Seyama, Yoshimasa Kamei, Takayuki Iriyama, Shinya Imada, Mari Ichinose, Masatake Toshimitsu, Tomoyuki Fujii, Hiroaki Asou
AIM: Antenatal maternal administration of magnesium sulfate (MgSO4 ) reduces cerebral palsy in preterm infants. However, it remains controversial as to whether it also reduces occurrence of white matter damage, or periventricular leukomalacia. We assessed the effect of MgSO4 against white matter damage induced by hypoxic-ischemic insult using a neonatal rat model and culture of premyelinating oligodendrocytes (pre-OL). METHODS: Rat pups at postnatal day (P) 6 were administered either MgSO4 or vehicle intraperitoneally before hypoxic-ischemic insult (unilateral ligation of the carotid artery followed by 6% oxygen for 1 h)...
January 23, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29355709/magnesium-sulphate-induces-preconditioning-in-preterm-rodent-models-of-cerebral-hypoxia-ischemia
#3
Gabriella Koning, Ellinor Lyngfelt, Pernilla Svedin, Anna-Lena Leverin, Masako Jinnai, Pierre Gressens, Claire Thornton, Xiaoyang Wang, Carina Mallard, Henrik Hagberg
BACKGROUND: Brain injury in preterm infants represents a substantial clinical problem associated with development of motor impairment, cognitive deficits and psychiatric problems. According to clinical studies, magnesium sulphate (MgSO4) given to women in preterm labor reduces the risk of cerebral palsy in the offspring but the mechanisms behind its neuroprotective effects are still unclear. Our aim was to explore whether MgSO4 induces tolerance (preconditioning) in the preterm rodent brain...
January 17, 2018: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/29286442/neurobehavioral-assessments-in-a-mouse-model-of-neonatal-hypoxic-ischemic-brain-injury
#4
MinGi Kim, Ji Hea Yu, Jung Hwa Seo, Yoon-Kyum Shin, Soohyun Wi, Ahreum Baek, Suk-Young Song, Sung-Rae Cho
We performed unilateral carotid artery occlusion on CD-1 mice to create a neonatal hypoxic-ischemic (HI) model and investigated the effects of neonatal HI brain injury by studying neurobehavioral functions in these mice compared to non-operated (i.e., normal) mice. During the study, Rice-Vannucci's method was used to induce neonatal HI brain damage in postnatal day 7-10 (P7-10) mice. The HI operation was performed on the pups by unilateral carotid artery ligation and exposure to hypoxia (8% O2 and 92% N2 for 90 min)...
November 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29203170/diameter-of-fluorescent-microspheres-determines-their-distribution-throughout-the-cortical-watershed-area-in-mice
#5
Naoki Tsukada, Masahiro Katsumata, Koichi Oki, Kazushi Minami, Takato Abe, Shinichi Takahashi, Yoshiaki Itoh, Norihiro Suzuki
A hemodynamic mechanism has long been assumed to play an important role in watershed infarction. In recent years, however, clinical evidence has indicated that an embolic mechanism is involved. The mechanism by which emboli are trapped preferentially in watershed areas remains unclear. In the present study, we developed a mouse embolus model using fluorescent microspheres with different diameters and evaluated the role of the microspheres' diameters in the generation of a watershed-patterned distribution. We injected fluorescent microspheres of four different diameters (i...
December 1, 2017: Brain Research
https://www.readbyqxmd.com/read/29080545/increased-serum-trem-1-level-is-associated-with-in-stent-restenosis-and-activation-of-trem-1-promotes-inflammation-proliferation-and-migration-in-vascular-smooth-muscle-cells
#6
Fang Wang, Chang Li, Feng Hua Ding, Ying Shen, Jie Gao, Zhu Hui Liu, Jia Wei Chen, Rui Yan Zhang, Wei Feng Shen, Xiao Qun Wang, Lin Lu
BACKGROUND AND AIMS: In-stent restenosis (ISR) remains a major limitation of percutaneous coronary intervention despite improvements in stent design and pharmacological agents, whereas the mechanism of ISR has not been fully clarified. In the present study, we sought to investigate the potential association of serum soluble TREM-1 (sTREM-1) levels with the incidence of ISR. The role of TREM-1 was evaluated in cultured vascular smooth muscle cells (VSMCs). METHODS: Out of 1683 patients undergoing coronary intervention and follow-up coronary angiography after approximately one year, 130 patients were diagnosed with ISR, and 150 gender- and age-matched patients with no ISR were randomly included as controls...
October 14, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29066981/hydrogen-sulfide-attenuates-atherosclerosis-in-a-partially-ligated-carotid-artery-mouse-model-via-regulating-angiotensin-converting-enzyme-2-expression
#7
Yanjun Lin, Huasu Zeng, Lin Gao, Ting Gu, Changqian Wang, Huili Zhang
Hydrogen sulfide has been suggested to play an essential role in atherogenesis. There is a paucity of information about the association between H2S and angiotensin converting enzyme 2 (ACE2), a novel homolog of ACE. Therefore, the aim of the study was to explore the role of H2S in atherosclerosis with respect to ACE2 both in vitro and in vivo. Here, a murine model of acutely disturbed flow-induced atherosclerosis by left common carotid artery (LCA) partial ligation was utilized. We found that carotid partial ligation in high-fat fed apoE(-/-) mice significantly inhibited endogenous H2S synthesis in LCA...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29053988/intermedin-reduces-neointima-formation-by-regulating-vascular-smooth-muscle-cell-phenotype-via-camp-pka-pathway
#8
Qing Zhu, Xian-Qiang Ni, Wei-Wei Lu, Jin-Sheng Zhang, Jin-Ling Ren, Di Wu, Yao Chen, Lin-Shuang Zhang, Yan-Rong Yu, Chao-Shu Tang, Yong-Fen Qi
BACKGROUND AND AIMS: Vascular smooth muscle cell (VSMC) dedifferentiation contributes to neointima formation, which results in various vascular disorders. Intermedin (IMD), a cardiovascular paracrine/autocrine polypeptide, is involved in maintaining circulatory homeostasis. However, whether IMD protects against neointima formation remains largely unknown. The purpose of this study is to investigate the role of IMD in neointima formation and the possible mechanism. METHODS: IMD1-53 (100ng/kg/h) or saline water was used on rat carotid-artery balloon-injury model...
November 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29025747/uncoupling-protein-2-inhibits-myointimal-hyperplasia-in-preclinical-animal-models-of-vascular-injury
#9
Yan Zhang, Yaolei Zhang, Wei Li, Peijian Wang, Rui Gu, Yaxing Feng, Shujie Wei, Ke Peng, Yunrong Zhang, Linan Su, Qiang Wang, De Li, Dachun Yang, Wing Tak Wong, Yongjian Yang, Shuangtao Ma
BACKGROUND: Intracoronary stent restenosis, characterized by excessive smooth muscle cell (SMC) proliferation and myointimal hyperplasia, remains a clinical challenge. Mitochondrial membrane potential has been linked to the proliferative rate of SMCs. This study aimed to screen a critical gene regulating mitochondrial potential and to confirm its effects on myointimal formation in preclinical animal models. METHODS AND RESULTS: We performed transcriptome screening for genes differentially expressed in ligated versus unligated mouse carotid arteries...
October 12, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29016743/semaphorin-3e-attenuates-neointimal-formation-via-suppressing-vsmcs-migration-and-proliferation
#10
Jie-Hong Wu, Yanan Li, Yi-Fan Zhou, James Haslam, Opoku Nana Elvis, Ling Mao, Yuan-Peng Xia, Bo Hu
Aims: The migration and proliferation of vascular smooth muscle cells (VSMCs) are crucial events in the neointimal formation, a hallmark of atherosclerosis and restenosis. Semaphorin3E (Sema3E) has been found to be a critical regulator of cell migration and proliferation in many scenarios. However, its role on VSMCs migration and proliferation is unclear. This study aimed to investigate the effect of Sema3E on VSMCs migration, proliferation and neointimal formation, and explore possible mechanisms...
September 15, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28935983/model-based-vascular-elastography-improves-the-detection-of-flow-induced-carotid-artery-remodeling-in-mice
#11
Vyacheslav A Korshunov, Hexuan Wang, Rifat Ahmed, Deanne M Mickelsen, Qian Zhou, Chen Yan, Marvin M Doyley
Increased arterial thickness measured with ultrasound correlates with future cardiovascular events, but conventional ultrasound imaging techniques cannot distinguish between intima, media, or atherosclerotic plaque in the carotid artery. In this work, we evaluated how well vascular elastography can detect intimal changes in a mouse model of carotid remodeling. We ligated the left external and internal branches of the carotid artery of male FVB mice and performed sham operations for 2 weeks. High-resolution ultrasound imaging accurately detected lower blood velocities and low blood volume flow in the carotid arteries after ligation in FVB mice...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28832962/rubiarbonone-c-inhibits-platelet-derived-growth-factor-induced-proliferation-and-migration-of-vascular-smooth-muscle-cells-through-the-focal-adhesion-kinase-mapk-and-stat3-tyr-705-signalling-pathways
#12
Hyun-Soo Park, Khong Trong Quan, Joo-Hui Han, Sang-Hyuk Jung, Do-Hyung Lee, Eunji Jo, Tae-Wan Lim, Kyung-Sun Heo, MinKyun Na, Chang-Seon Myung
BACKGROUND AND PURPOSE: The proliferation and migration of vascular smooth muscle cells (VSMCs) induced by platelet-derived growth factor (PDGF) are important steps in cardiovascular diseases, including neointimal lesion formation, myocardial infarction and atherosclerosis. Here, we evaluated the rubiarbonone C-mediated signalling pathways that regulate PDGF-induced VSMC proliferation and migration. EXPERIMENTAL APPROACH: Cell proliferation and migration were measured in cells treated with rubiarbonone C followed by PDGF BB using the MTT assay, [(3) H]-thymidine incorporation, flow cytometry and wound-healing migration assay, MMP gelatin zymography, a fluorescence assay for F-actin...
November 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28634464/anti-inflammatory-and-neuroprotective-effects-of-co-ultrapealut-in-a-mouse-model-of-vascular-dementia
#13
Rosalba Siracusa, Daniela Impellizzeri, Marika Cordaro, Rosalia Crupi, Emanuela Esposito, Stefania Petrosino, Salvatore Cuzzocrea
Vascular dementia (VaD), the second most common cause of cognitive impairment in the population, is a disease that results from reduction in regional cerebral blood flow and involves oxidative stress and inflammation. Co-ultramicronized PEALut (co-ultra PEALut) is a new compound with beneficial effects, which include anti-inflammatory and antioxidant properties. Recently, co-ultraPEALut has been shown to exhibit neuroprotective effects in models of Parkinson's disease, cerebral ischemia and Alzheimer's disease...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28546219/inos-derived-nitric-oxide-induces-integrin-linked-kinase-endocytic-lysosome-mediated-degradation-in-the-vascular-endothelium
#14
Paula Reventun, Matilde Alique, Irene Cuadrado, Susana Márquez, Rocío Toro, Carlos Zaragoza, Marta Saura
OBJECTIVE: ILK (integrin-linked kinase) plays a key role in controlling vasomotor tone and is decreased in atherosclerosis. The objective of this study is to test whether nitric oxide (NO) regulates ILK in vascular remodeling. APPROACH AND RESULTS: We found a striking correlation between increased levels of inducible nitric oxide and decreased ILK levels in human atherosclerosis and in a mouse model of vascular remodeling (carotid artery ligation) comparing with iNOS (inducible NO synthase) knockout mice...
July 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28540322/targeting-wnt-%C3%AE-catenin-activated-cells-with-dominant-negative-n-cadherin-to-reduce-neointima-formation
#15
Sarah Hulin-Curtis, Helen Williams, Kerry S Wadey, Graciela B Sala-Newby, Sarah J George
Approximately 50% of coronary artery bypass grafts using the autologous saphenous vein fail within 10 years due to intimal thickening. This study examined whether a gene therapy approach that selectively kills Wnt/β-catenin/T cell factor (TCF) activated vascular smooth muscle cells (VSMCs) using dominant-negative N-cadherin (dn-N-cadherin) reduced intimal thickening. Cultured human VSMCs infected with an adenovirus (Ad) encoding dn-N-cadherin via the TCF promoter (Ad-TOP-dn-N-cadherin) specifically expressed dn-N-cadherin in response to activation of the Wnt/β-catenin/TCF pathway...
June 16, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28449099/connexin37-reduces-smooth-muscle-cell-proliferation-and-intimal-hyperplasia-in-a-mouse-model-of-carotid-artery-ligation
#16
Florent Allagnat, Céline Dubuis, Martine Lambelet, Loïc Le Gal, Florian Alonso, Jean-Marc Corpataux, Sébastien Déglise, Jacques-Antoine Haefliger
Aims: Intimal hyperplasia (IH) is an abnormal response to vessel injury characterized by the dedifferentiation, migration, and proliferation of quiescent vascular smooth muscle cells (VSMC) to form a neointima layer. Vascular connexins (Cx) are involved in the pathophysiology of various vascular diseases, and Cx43, the main Cx expressed in VSMC, has been shown to promote VSMC proliferation and IH. The aim of this study was to investigate the participation of another Cx, namely Cx37, in the formation of the neointima layer...
June 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28447728/high-expression-of-ubiquitin-specific-peptidase-39-is-associated-with-the-development-of-vascular-remodeling
#17
Shuai He, Wei Zhong, Li Yin, Yifei Wang, Zhibing Qiu, Gang Song
Vascular remodeling is the primary cause underlying the failure of angioplasty surgeries, including vascular stenting, transplant vasculopathy and vein grafts. Multiple restenosis‑associated proteins and genes have been identified to account for this. In the present study, the functions of ubiquitin‑specific peptidase 39 (USP39) were investigated in the context of two vascular remodeling models (a mouse common carotid artery ligation and a pig bilateral saphenous vein‑carotid artery interposition graft)...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28406931/a-model-of-periventricular-leukomalacia-pvl-in-neonate-mice-with-histopathological-and-neurodevelopmental-outcomes-mimicking-human-pvl-in-neonates
#18
Nahla Zaghloul, Hardik Patel, Mohamed Nagy Ahmed
Periventricular leukomalacia (PVL), a brain injury affecting premature infants is commonly associated with cerebral palsy. PVL results from hypoxia-ischemia (HI) with or without infection and is characterized by white matter necrotic lesions, hypomyelination, microglial activation, astrogliosis, and neuronal death. It is important to study a PVL mouse model that mimics human PVL in symptomatology, anatomic and molecular basis. In our neonate mice model, bilateral carotid arteries were temporary ligated at P5 followed by hypoxic exposure (FiO2 of 8% for 20 min...
2017: PloS One
https://www.readbyqxmd.com/read/28348545/extracerebral-tissue-damage-in-the-intraluminal-filament-mouse-model-of-middle-cerebral-artery-occlusion
#19
Markus Vaas, Ruiqing Ni, Markus Rudin, Anja Kipar, Jan Klohs
Middle cerebral artery occlusion is the most common model of focal cerebral ischemia in the mouse. In the surgical procedure, the external carotid artery (ECA) is ligated; however, its effect on the tissue supplied by the vessel has not been described so far. C57BL/6 mice underwent 1 h of transient MCAO (tMCAO) or sham surgery. Multi-spectral optoacoustic tomography was employed at 30 min after surgery to assess oxygenation in the temporal muscles. Microstructural changes were assessed with magnetic resonance imaging and histological examination at 24 h and 48 h after surgery...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28258189/selective-expression-of-tspan2-in-vascular-smooth-muscle-is-independently-regulated-by-tgf-%C3%AE-1-smad-and-myocardin-serum-response-factor
#20
Jinjing Zhao, Wen Wu, Wei Zhang, Yao Wei Lu, Emiley Tou, Jiemei Ye, Ping Gao, David Jourd'heuil, Harold A Singer, Mingfu Wu, Xiaochun Long
Tetraspanins (TSPANs) comprise a large family of 4-transmembrane domain proteins. The importance of TSPANs in vascular smooth muscle cells (VSMCs) is unexplored. Given that TGF-β1 and myocardin (MYOCD) are potent activators for VSMC differentiation, we screened for TGF-β1 and MYOCD/serum response factor (SRF)-regulated TSPANs in VSMC by using RNA-seq analyses and RNA-arrays. TSPAN2 was found to be the only TSPAN family gene induced by TGF-β1 and MYOCD, and reduced by SRF deficiency in VSMCs. We also found that TSPAN2 is highly expressed in smooth muscle-enriched tissues and down-regulated in in vitro models of VSMC phenotypic modulation...
June 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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