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carotid ligation mouse model

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https://www.readbyqxmd.com/read/27830723/inhibition-of-epac1-suppresses-mitochondrial-fission-and-reduces-neointima-formation-induced-by-vascular-injury
#1
Hui Wang, William G Robichaux, Ziqing Wang, Fang C Mei, Ming Cai, Guangwei Du, Ju Chen, Xiaodong Cheng
Vascular smooth muscle cell (VSMC) activation in response to injury plays an important role in the development of vascular proliferative diseases, including restenosis and atherosclerosis. The aims of this study were to ascertain the physiological functions of exchange proteins directly activated by cAMP isoform 1 (Epac1) in VSMC and to evaluate the potential of Epac1 as therapeutic targets for neointima formation during vascular remodeling. In a mouse carotid artery ligation model, genetic knockdown of the Epac1 gene led to a significant reduction in neointima obstruction in response to vascular injury...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27582556/blockade-of-urotensin-ii-receptor-prevents-vascular-dysfunction
#2
Young-Ae Kim, Dong Gil Lee, Kyu Yang Yi, Byung Ho Lee, Yi-Sook Jung
Urotensin II (UII) is a potent vasoactive peptide and mitogenic agent to induce proliferation of various cells including vascular smooth muscle cells (VSMCs). In this study, we examined the effects of a novel UII receptor (UT) antagonist, KR-36676, on vasoconstriction of aorta and proliferation of aortic SMCs. In rat aorta, UII-induced vasoconstriction was significantly inhibited by KR-36676 in a concentration-dependent manner. In primary human aortic SMCs (hAoSMCs), UII-induced cell proliferation was significantly inhibited by KR-36676 in a concentration-dependent manner...
September 1, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27355581/protective-effect-of-irisin-on-atherosclerosis-via-suppressing-oxidized-low-density-lipoprotein-induced-vascular-inflammation-and-endothelial-dysfunction
#3
Yuzhu Zhang, Qian Mu, Zheng Zhou, Haibo Song, Yuan Zhang, Fei Wu, Miao Jiang, Fang Wang, Wen Zhang, Liang Li, Lei Shao, Xingli Wang, Shiwu Li, Lijun Yang, Qi Wu, Mingxiang Zhang, Dongqi Tang
Irisin, a newly discovered myokine, is considered as a promising candidate for the treatment of metabolic disturbances and cardiovascular diseases. In the present study, we used two animal models, apolipoprotein E-deficient mice fed on a high-cholesterol diet and a mouse carotid partial ligation model to test the anti-atherosclerotic effect of irisin. Irisin treatment (0.5 μg/g body weight/day) significantly reduced the severity of aortic atherosclerosis in apolipoprotein E-deficient mice fed on a high-cholesterol diet and suppressed carotid neointima formation in a carotid partial ligation model...
2016: PloS One
https://www.readbyqxmd.com/read/27173134/discovery-of-novel-peptides-targeting-pro-atherogenic-endothelium-in-disturbed-flow-regions-targeted-sirna-delivery-to-pro-atherogenic-endothelium-in-vivo
#4
Jihwa Chung, Hyunbo Shim, Kwanchang Kim, Duhwan Lee, Won Jong Kim, Dong Hoon Kang, Sang Won Kang, Hanjoong Jo, Kihwan Kwon
Atherosclerosis occurs preferentially in arterial regions exposed to disturbed blood flow. Targeting these pro-atherogenic regions is a potential anti-atherogenic therapeutic approach, but it has been extremely challenging. Here, using in vivo phage display approach and the partial carotid ligation model of flow-induced atherosclerosis in mouse, we identified novel peptides that specifically bind to endothelial cells (ECs) exposed to disturbed flow condition in pro-atherogenic regions. Two peptides, CLIRRTSIC and CPRRSHPIC, selectively bound to arterial ECs exposed to disturbed flow not only in the partially ligated carotids but also in the lesser curvature and branching point of the aortic arch in mice as well as human pulmonary artery branches...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27006011/nitric-oxide-deficit-drives-intimal-hyperplasia-in-mouse-models-of-hypertension
#5
F Allagnat, J-A Haefliger, M Lambelet, A Longchamp, X Bérard, L Mazzolai, J-M Corpataux, S Déglise
OBJECTIVE: To evaluate the impact of different types of hypertension on the development of intimal hyperplasia (IH). METHOD: Genetic, surgical, and pharmacological models of hypertension were used to compare IH formation in a murine model of carotid artery ligation (CAL). CAL was performed in normotensive WT male mice and in three mouse models of hypertension: (1) L-NAME (Nω-nitro-l-arginine-methyl-ester) treatment for 2 weeks prior to CAL to instate renin-independent hypertension; (2) 2K1C (two kidneys, one clip) surgery 1 week prior to CAL to induce renin-dependent hypertension; (3) Cx40-/- mice, a genetic model of renin-dependent hypertension...
May 2016: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/26994041/in%C3%A2-vivo-evidence-for-long-term-vascular-remodeling-resulting-from-chronic-cerebral-hypoperfusion-in-mice
#6
Tom Struys, Kristof Govaerts, Wouter Oosterlinck, Cindy Casteels, Annelies Bronckaers, Michel Koole, Koen Van Laere, Paul Herijgers, Ivo Lambrichts, Uwe Himmelreich, Tom Dresselaers
We have characterized both acute and long-term vascular and metabolic effects of unilateral common carotid artery occlusion in mice by in vivo magnetic resonance imaging and positron emission tomography. This common carotid artery occlusion model induces chronic cerebral hypoperfusion and is therefore relevant to both preclinical stroke studies, where it serves as a control condition for a commonly used mouse model of ischemic stroke, and neurodegeneration, as chronic hypoperfusion is causative to cognitive decline...
March 18, 2016: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/26646502/placental-growth-factor-deficiency-is-associated-with-impaired-cerebral-vascular-development-in-mice
#7
Rayana Leal Luna, Vanessa R Kay, Matthew T Rätsep, Kasra Khalaj, Mallikarjun Bidarimath, Nichole Peterson, Peter Carmeliet, Albert Jin, B Anne Croy
STUDY HYPOTHESIS: Placental growth factor (PGF) is expressed in the developing mouse brain and contributes to vascularization and vessel patterning. STUDY FINDING: PGF is dynamically expressed in fetal mouse brain, particularly forebrain, and is essential for normal cerebrovascular development. WHAT IS KNOWN ALREADY: PGF rises in maternal plasma over normal human and mouse pregnancy but is low in many women with the acute onset hypertensive syndrome, pre-eclampsia (PE)...
February 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/26639507/effect-of-creatine-monohydrate-supplementation-on-relative-serum-level-of-il-6-and-il-18-following-neonatal-hypoxia-ischemia-in-male-albino-mouse
#8
Shahid Iqbal, Muhammad Ali, Furhan Iqbal
IL-6 has been reported to have neuroprotective effects against cerebral ischemia while IL-8 is a pro inflammatory cytokine structurally related to interleukin-1 family. In the present study, we tried to determine whether 2% Creatine monohydrate supplementation for variable duration influence the IL-6 and 18 concentrations in the serum of male albino mouse following right common carotid artery ligation and hypoxia (8% oxygen) for 25 minutes. Our result revealed that serum concentration of IL6 (P=0.0001) as well as IL-18 (P=0...
November 2015: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/26609155/ischemic-preconditioning-in-white-matter-magnitude-and-mechanism
#9
Margaret A Hamner, Zucheng Ye, Richard V Lee, Jamie R Colman, Thu Le, Davin C Gong, Bruce R Ransom, Jonathan R Weinstein
UNLABELLED: Ischemic preconditioning (IPC) is a robust neuroprotective phenomenon whereby brief ischemic exposure confers tolerance to a subsequent ischemic challenge. IPC has not been studied selectively in CNS white matter (WM), although stroke frequently involves WM. We determined whether IPC is present in WM and, if so, its mechanism. We delivered a brief in vivo preconditioning ischemic insult (unilateral common carotid artery ligation) to 12- to 14-week-old mice and determined WM ischemic vulnerability [oxygen-glucose deprivation (OGD)] 72 h later, using acutely isolated optic nerves (CNS WM tracts) from the preconditioned (ipsilateral) and control (contralateral) hemispheres...
November 25, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/26526209/modeling-transient-retinal-ischemia-in-mouse-by-ligation-of-pterygopalatine-artery
#10
Gillipsie Minhas, Ryuichi Morishita, Munehisa Shimamura, Reema Bansal, Akshay Anand
BACKGROUND: Retinal ischemia is a major cause of visual impairment and blindness worldwide. The available therapeutic strategies have limited potential. PURPOSE: In order to understand the pathophysiology and validating therapies for retinal ischemia, establishment of reproducible animal models is necessary. METHODS: In the model discussed in this article, the pterygopalatine artery (PPA) is ligated along with the external carotid artery for 3...
October 2015: Annals of Neurosciences
https://www.readbyqxmd.com/read/26506509/cd73-regulates-vascular-smooth-muscle-cell-functions-and-facilitates-atherosclerotic-plaque-formation
#11
Jiayin Yang, Rongrong Jian, Jiangang Yu, Xiuling Zhi, Xiaohong Liao, Jerry Yu, Ping Zhou
Extracellular adenosine, generated by ecto-5'-nucleotidase (CD73) via enzymatic catalyzation, has been found to facilitate atherosclerosis (AS). Thus, suppressing CD73 may attenuate AS. In this study, we evaluated the role of CD73 during AS development and further explored cellular and molecular mechanism in smooth muscle cells (SMCs). In a mouse model of carotid artery ligation, inactivation of CD73 inhibited migration and proliferation of vascular SMCs. In in vitro experiments, RNA interference of CD73 inhibited migration, proliferation, and foam cell transformation of human umbilical artery smooth muscle cells...
November 2015: IUBMB Life
https://www.readbyqxmd.com/read/26498924/the-orphan-nuclear-receptor-nur77-inhibits-low-shear-stress-induced-carotid-artery-remodeling-in-mice
#12
Ying Yu, Zhaohua Cai, Mingli Cui, Peng Nie, Zhe Sun, Shiqun Sun, Shichun Chu, Xiaolei Wang, Liuhua Hu, Jing Yi, Linghong Shen, Ben He
Shear stress, particularly low and oscillatory shear stress, plays a critical pathophysiological role in vascular remodeling-related cardiovascular diseases. Growing evidence suggests that the orphan nuclear receptor Nur77 [also known as TR3 or nuclear receptor subfamily 4, group A, member 1 (NR4A1)] is expressed in diseased human vascular tissue and plays an important role in vascular physiology and pathology. In the present study, we used a mouse model of flow-dependent remodeling by partial ligation of the left common carotid artery (LCCA) to define the exact role of Nur77 in vascular remodeling induced by low shear stress...
December 2015: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/26458725/fasudil-a-rho%C3%A2-kinase-inhibitor-prevents-intima%C3%A2-media-thickening-in-a-partially-ligated-carotid-artery-mouse-model-effects-of-fasudil-in-flow%C3%A2-induced-vascular-remodeling
#13
Xiangyu Zhang, Tao Zhang, Fu Gao, Qingle Li, Chenyang Shen, Yankui Li, Wei Li, Xiaoming Zhang
Vascular remodeling in response to hemodynamic alterations is a physiological process that requires coordinated signaling between endothelial, inflammatory and vascular smooth muscle cells (VSMCs). Extensive experimental and clinical studies have indicated the critical role of the Ras homolog gene family, member A/Rho‑associated kinase (ROCK) signaling pathway in the pathogenesis of cardiovascular disease, where ROCK activation has been demonstrated to promote inflammation and remodeling through inducing the expression of proinflammatory cytokines and adhesion molecules in endothelial cells and VSMCs...
November 2015: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26452067/acute-trkb-inhibition-rescues-phenobarbital-resistant-seizures-in-a-mouse-model-of-neonatal-ischemia
#14
S K Kang, M V Johnston, S D Kadam
Neonatal seizures are commonly associated with hypoxic-ischemic encephalopathy. Phenobarbital (PB) resistance is common and poses a serious challenge in clinical management. Using a newly characterized neonatal mouse model of ischemic seizures, this study investigated a novel strategy for rescuing PB resistance. A small-molecule TrkB antagonist, ANA12, used to selectively and transiently block post-ischemic BDNF-TrkB signaling in vivo, determined whether rescuing TrkB-mediated post-ischemic degradation of the K(+)-Cl(-) co-transporter (KCC2) rescued PB-resistant seizures...
November 2015: European Journal of Neuroscience
https://www.readbyqxmd.com/read/26330828/electroacupuncture-preconditioning-attenuates-ischemic-brain-injury-by-activation-of-the-adenosine-monophosphate-activated-protein-kinase-signaling-pathway
#15
Qiang-Qiang Ran, Huai-Long Chen, Yan-Li Liu, Hai-Xia Yu, Fei Shi, Ming-Shan Wang
Electroacupuncture has therapeutic effects on ischemic brain injury, but its mechanism is still poorly understood. In this study, mice were stimulated by electroacupuncture at the Baihui (GV20) acupoint for 30 minutes at 1 mA and 2/15 Hz for 5 consecutive days. A cerebral ischemia model was established by ligating the bilateral common carotid artery for 15 minutes. At 72 hours after injury, neuronal injury in the mouse hippocampus had lessened, and the number of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling-positive cells reduced after electroacupuncture treatment...
July 2015: Neural Regeneration Research
https://www.readbyqxmd.com/read/26254337/chemerin-promotes-the-proliferation-and-migration-of-vascular-smooth-muscle-and-increases-mouse-blood-pressure
#16
Hidemizu Kunimoto, Kyosuke Kazama, Mizuho Takai, Mayuko Oda, Muneyoshi Okada, Hideyuki Yamawaki
Blood chemerin concentration shows positive correlation not only with body mass index and serum triglyceride level but also with systolic blood pressure. While it seems likely that chemerin influences vascular smooth muscle cell (SMC) proliferation and migration, which are crucial to the development of hypertension, this remains to be clarified. In the present study, we investigated whether chemerin controls SMC proliferation and migration in vitro and also affects blood pressure in vivo. In vitro, chemerin significantly stimulated rat mesenteric arterial SMC proliferation and migration, as determined by a cell counting assay and Boyden chamber assay, respectively...
September 2015: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/26252890/evaluation-of-the-bdca2-dtr-transgenic-mouse-model-in-chronic-and-acute-inflammation
#17
Manuela Mandl, Maik Drechsler, Yvonne Jansen, Carlos Neideck, Heidi Noels, Alexander Faussner, Oliver Soehnlein, Christian Weber, Yvonne Döring
BACKGROUND AND AIMS: Plasmacytoid dendritic cells (pDCs) are a small subset of dendritic cells and the main producers of type I interferons. Besides their contribution to tolerance, they are known to be involved in autoimmune diseases and have recently been implicated in atherosclerosis. However, their precise involvement, particularly in advanced lesion development, remains elusive. Hence, we investigated the role of pDCs in atherogenesis vs atheroprogression by specifically depleting this cell population using the BDCA2-DTR mouse model bred to Apolipoprotein E (Apoe-/-) deficient mice...
2015: PloS One
https://www.readbyqxmd.com/read/26125287/external-compression-versus-intravascular-injection-a-mechanistic-animal-model-of-filler-induced-tissue-ischemia
#18
Shu-Hong Chang, Sivash Yousefi, Jia Qin, Kristin Tarbet, Suzan Dziennis, Ruikang Wang, Michael C Chappell
PURPOSE: Soft tissue ischemia is a devastating and unpredictable complication following dermal filler injection. Multiple mechanisms to explain this complication have been proposed, including vascular compression, vessel damage, and intraarterial filler emboli. To elucidate the mechanism of injury, the authors introduce a mouse model, imaged with optical microangiography and laser speckle contrast imaging technologies, to demonstrate in vivo microvascular response to soft tissue and intravascular filler injection...
July 2016: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/26121751/flow-dependent-expression-of-ectonucleotide-tri-di-phosphohydrolase-1-and-suppression-of-atherosclerosis
#19
Yogendra Kanthi, Matthew C Hyman, Hui Liao, Amy E Baek, Scott H Visovatti, Nadia R Sutton, Sascha N Goonewardena, Mithun K Neral, Hanjoong Jo, David J Pinsky
The ability of cells to detect and respond to nucleotide signals in the local microenvironment is essential for vascular homeostasis. The enzyme ectonucleotide tri(di)phosphohydrolase-1 (ENTPD1, also known as CD39) on the surface of leukocytes and endothelial cells metabolizes locally released, intravascular ATP and ADP, thereby eliminating these prothrombotic and proinflammatory stimuli. Here, we evaluated the contribution of CD39 to atherogenesis in the apolipoprotein E-deficient (ApoE-deficient) mouse model of atherosclerosis...
August 3, 2015: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/26086218/micro-ct-technique-is-well-suited-for-documentation-of-remodeling-processes-in-murine-carotid-arteries
#20
Christoph Schürmann, Felix Gremse, Hanjoong Jo, Fabian Kiessling, Ralf P Brandes
BACKGROUND: The pathomechanisms of atherosclerosis and vascular remodelling are under intense research. Only a few in vivo tools to study these processes longitudinally in animal experiments are available. Here, we evaluated the potential of micro-CT technology. METHODS: Lumen areas of the common carotid arteries (CCA) in the ApoE-/- partial carotid artery ligation mouse model were compared between in vivo and ex vivo micro-CT technique and serial histology in a total of 28 animals...
2015: PloS One
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