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vascular smooth muscle cell clonality and senescence

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https://www.readbyqxmd.com/read/7019665/cell-culture-aging
#1
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M Reff, E L Schneider
Cellular research in aging has been stimulated by the observation that human diploid cells have a limited number of cell divisions in culture. This loss of cellular proliferation (in vitro senescence) has been extensively studied by biochemical, clonal, and genetic analysis. Studies of human skin fibroblast cultures have revealed that in vitro senescense is related to in vivo human cellular aging. Recently differentiated cells have been proposed for aging studies. These cells may provide additional information on aging since alterations of in vitro cellular functions may be related to the in vivo behavior of specific differented cell types...
May 26, 1981: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/7012162/proliferative-characteristics-of-clonal-endothelial-cell-strains
#2
E M Rosen, S N Mueller, J P Noveral, E M Levine
We have utilized clonal strains of bovine fetal aortic endothelial cells to study cellular senescence in a differentiated cell type of physiological significance. Serial subcultivation of nine endothelial clones derived from three fetal calf aortas revealed proliferative life-spans in vitro of 53--125 population doublings (PDs), compared with 60 and 143 PDs for two lines of bovine fetal lung cells and 85 and 147 PDs for two lines of bovine vascular smooth muscle cells. Serial growth curves showed marked reductions associated with endothelial cellular senescence both in cellular growth rate and culture plateau density...
April 1981: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/1311088/immortalization-of-primary-human-smooth-muscle-cells
#3
N Perez-Reyes, C L Halbert, P P Smith, E P Benditt, J K McDougall
Primary human aortic and myometrial smooth muscle cells (SMCs) were immortalized using an amphotropic recombinant retroviral construct containing the E6 and E7 open reading frames (ORFs) of human papillomavirus type 16. The SMCs expressing the E6/E7 ORFs have considerably elevated growth rates when compared with nonimmortalized control cells and show no signs of senescence with long-term passage. The first SMC line derived in this study has been maintained in continuous tissue culture for greater than 1 year (greater than 180 population doublings)...
February 15, 1992: Proceedings of the National Academy of Sciences of the United States of America
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