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vascular smooth muscle cell clonality

Boon-Seng Soh, Shi-Yan Ng, Hao Wu, Kristina Buac, Joo-Hye C Park, Xiaojun Lian, Jiejia Xu, Kylie S Foo, Ulrika Felldin, Xiaobing He, Massimo Nichane, Henry Yang, Lei Bu, Ronald A Li, Bing Lim, Kenneth R Chien
Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins...
2016: Nature Communications
Abdul Q Sheikh, Ashish Misra, Ivan O Rosas, Ralf H Adams, Daniel M Greif
Excess and ectopic smooth muscle cells (SMCs) are central to cardiovascular disease pathogenesis, but underlying mechanisms are poorly defined. For instance, pulmonary hypertension (PH) or elevated pulmonary artery blood pressure is a devastating disease with distal extension of smooth muscle to normally unmuscularized pulmonary arterioles. We identify novel SMC progenitors that are located at the pulmonary arteriole muscular-unmuscular border and express both SMC markers and the undifferentiated mesenchyme marker platelet-derived growth factor receptor-β (PDGFR-β)...
October 7, 2015: Science Translational Medicine
Gabriella Lania, Rosa Ferrentino, Antonio Baldini
The T-box transcription factor TBX1 has critical roles in maintaining proliferation and inhibiting differentiation of cardiac progenitor cells of the second heart field (SHF). Haploinsufficiency of the gene that encodes it is a cause of congenital heart disease. Here, we developed an embryonic stem (ES) cell-based model in which Tbx1 expression can be modulated by tetracycline. Using this model, we found that TBX1 down regulates the expression of VEGFR2, and we confirmed this finding in vivo during embryonic development...
2015: PloS One
M B Gravanis, R Waksman
Although the precise pathogenesis of restenosis after percutaneous transluminal coronary angioplasty (PTCA) remains somewhat elusive, our understanding about the reparative phenomena at the site of dilatation has been significantly improved in recent years. Thus, restenosis appears to be the result of migration, proliferation, and excessive matrix formation by smooth muscle cells plus vascular wall remodeling leading to chronic recoil (constriction). Proposed pharmacotherapies to prevent restenosis have been ineffective in humans, in spite of a relative success in certain experimental animals...
January 1997: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Xiaojun Lian, Xiaoping Bao, Abraham Al-Ahmad, Jialu Liu, Yue Wu, Wentao Dong, Kaitlin K Dunn, Eric V Shusta, Sean P Palecek
Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors may provide the means for vascularization of tissue-engineered constructs and can serve as models to study vascular development and disease. Here, we report a method to efficiently produce endothelial cells from hPSCs via GSK3 inhibition and culture in defined media to direct hPSC differentiation to CD34(+)CD31(+) endothelial progenitors. Exogenous vascular endothelial growth factor (VEGF) treatment was dispensable, and endothelial progenitor differentiation was β-catenin dependent...
November 11, 2014: Stem Cell Reports
Susanne Feil, Birgit Fehrenbacher, Robert Lukowski, Frank Essmann, Klaus Schulze-Osthoff, Martin Schaller, Robert Feil
RATIONALE: Atherosclerosis is a widespread and devastating disease, but the origins of cells within atherosclerotic plaques are not well defined. OBJECTIVE: To investigate the specific contribution of vascular smooth muscle cells (SMCs) to atherosclerotic plaque formation by genetic inducible fate mapping in mice. METHODS AND RESULTS: Vascular SMCs were genetically pulse-labeled using the tamoxifen-dependent Cre recombinase, CreER(T2), expressed from the endogenous SM22α locus combined with Cre-activatable reporter genes that were integrated into the ROSA26 locus...
September 12, 2014: Circulation Research
Sarah J Holdsworth-Carson, Marina Zaitseva, Jane E Girling, Beverley J Vollenhoven, Peter A W Rogers
Uterine fibroids are a prevalent gynaecological condition in reproductive-aged women and are the commonest reason for hysterectomy. The cellular composition of clonal fibroids are heterogeneous, with phenotypically dissimilar cells that include smooth muscle cells (SMC), vascular SMC (VSMC) and fibroblasts. The aim of our study was to investigate genes that are commonly differentially expressed between fibroid and myometrial whole tissues in phenotypically different sub-populations of cells isolated from fibroid and myometrium...
May 2014: Reproduction: the Official Journal of the Society for the Study of Fertility
Sarah J Holdsworth-Carson, Marina Zaitseva, Beverley J Vollenhoven, Peter A W Rogers
Uterine fibroids are conventionally defined as clonally derived benign tumours from the proliferation of a single smooth muscle cell (SMC). We have previously identified fibroblast-like cells in fibroids, the presence of which raises the question as to whether all cells within the fibroid have the same clonal origin. The first aim of this study was to develop a fluorescence-activated cell sorting (FACS)-based method to isolate different cell types from human myometrium and fibroid tissues. Secondly, we aimed to use X chromosome inactivation analysis to determine the clonality of cell subpopulations isolated from myometrial and fibroid tissues...
March 2014: Molecular Human Reproduction
Nicolas Espagnolle, Fabien Guilloton, Frédéric Deschaseaux, Mélanie Gadelorge, Luc Sensébé, Philippe Bourin
Bone marrow mesenchymal stem cells (MSCs) are plastic adherent cells that can differentiate into various tissue lineages, including osteoblasts, adipocytes and chondrocytes. However, this progenitor property is not shared by all cells within the MSC population. In addition, MSCs vary in their proliferation capacity and expression of markers. Because of heterogeneity of CD146 expression in the MSC population, we compared CD146(-/Low) and CD146(High) cells under clonal conditions and after sorting of the non-clonal cell population to determine whether this expression is associated with specific functions...
January 2014: Journal of Cellular and Molecular Medicine
Kathy O Lui, Lior Zangi, Eduardo A Silva, Lei Bu, Makoto Sahara, Ronald A Li, David J Mooney, Kenneth R Chien
Distinct families of multipotent heart progenitors play a central role in the generation of diverse cardiac, smooth muscle and endothelial cell lineages during mammalian cardiogenesis. The identification of precise paracrine signals that drive the cell-fate decision of these multipotent progenitors, and the development of novel approaches to deliver these signals in vivo, are critical steps towards unlocking their regenerative therapeutic potential. Herein, we have identified a family of human cardiac endothelial intermediates located in outflow tract of the early human fetal hearts (OFT-ECs), characterized by coexpression of Isl1 and CD144/vWF...
October 2013: Cell Research
Saurabh Aggarwal, Christine M Gross, Shruti Sharma, Jeffrey R Fineman, Stephen M Black
The pathogenesis of pulmonary hypertension is a complex multifactorial process that involves the remodeling of pulmonary arteries. This remodeling process encompasses concentric medial thickening of small arterioles, neomuscularization of previously nonmuscular capillary-like vessels, and structural wall changes in larger pulmonary arteries. The pulmonary arterial muscularization is characterized by vascular smooth muscle cell hyperplasia and hypertrophy. In addition, in uncontrolled pulmonary hypertension, the clonal expansion of apoptosis-resistant endothelial cells leads to the formation of plexiform lesions...
July 2013: Comprehensive Physiology
Tien Peng, Ying Tian, Cornelis J Boogerd, Min Min Lu, Rachel S Kadzik, Kathleen M Stewart, Sylvia M Evans, Edward E Morrisey
Co-development of the cardiovascular and pulmonary systems is a recent evolutionary adaption to terrestrial life that couples cardiac output with the gas exchange function of the lung. Here we show that the murine pulmonary vasculature develops even in the absence of lung development. We have identified a population of multipotent cardiopulmonary mesoderm progenitors (CPPs) within the posterior pole of the heart that are marked by the expression of Wnt2, Gli1 and Isl1. We show that CPPs arise from cardiac progenitors before lung development...
August 29, 2013: Nature
C Bai, L Hou, M Zhang, L Wang, W Guan, Y Ma
OBJECTIVES: Many kinds of cardiac progenitor cell populations have been identified, including c-kit(+) , Nkx2.5(+) s and GATA4(+) cells. However, these progenitors have limited ability to differentiate into different cardiac cell types. Recently, a new kind of cardiac progenitor cell named the multipotent Isl1(+) cardiovascular progenitor (MICPs) has been identified, which also expresses Nkx2.5, GATA4, CD34 and Flk1. MATERIALS AND METHODS: In this study, we have isolated and characterized MICPs from chicken embryonic heart tissues using immunofluorescence and PCR...
April 2013: Cell Proliferation
Sun-Hwa Song, Woojin Jung, Koung Li Kim, Wonpyo Hong, Hyun Ok Kim, Kyung-Ah Lee, Ki-Young Lee, Wonhee Suh
Identification of differentially expressed genes in angioblasts derived from human embryonic stem cells (hESCs) is of great interest for elucidating the molecular mechanisms underlying human vasculogenesis. The aim of this study was to define hESC-derived angioblasts at the clonal level and to perform comparative transcriptional analysis to characterize their distinct gene expression profiles. In a clonal analysis performed in cell-specific differentiation media, hESC-derived CD34(+)CD31(+) cells were identified as angioblasts in that they exhibited a significantly higher ability to form endothelial cell (EC) and smooth muscle cell (SMC) colonies than CD34(+)CD31(-) and CD34(-) cell populations did...
May 1, 2013: Experimental Cell Research
Reza Ardehali, Shah R Ali, Matthew A Inlay, Oscar J Abilez, Michael Q Chen, Timothy A Blauwkamp, Masayuki Yazawa, Yongquan Gong, Roeland Nusse, Micha Drukker, Irving L Weissman
A goal of regenerative medicine is to identify cardiovascular progenitors from human ES cells (hESCs) that can functionally integrate into the human heart. Previous studies to evaluate the developmental potential of candidate hESC-derived progenitors have delivered these cells into murine and porcine cardiac tissue, with inconclusive evidence regarding the capacity of these human cells to physiologically engraft in xenotransplantation assays. Further, the potential of hESC-derived cardiovascular lineage cells to functionally couple to human myocardium remains untested and unknown...
February 26, 2013: Proceedings of the National Academy of Sciences of the United States of America
Olivier Goupille, Giorgia Pallafacchina, Frédéric Relaix, Simon J Conway, Ana Cumano, Benoit Robert, Didier Montarras, Margaret Buckingham
We report expression of Pax3, an important regulator of skeletal muscle stem cell behaviour, in the brachial and femoral arteries of adult mice. In these contractile arteries of the limb, but not in the elastic arteries of the trunk, bands of GFP-positive cells were observed in Pax3(GFP/+) mice. Histological and biochemical examination of the vessels, together with clonal analysis after purification of Pax3-GFP-positive cells by flow cytometry, established their vascular smooth muscle identity. These blood-vessel-derived cells do not respond to inducers of other mesodermal cell types, such as bone, however, they can contribute to muscle fibre formation when co-cultured with skeletal muscle cells...
December 1, 2011: Journal of Cell Science
William E Schutzer, Douglas R Beard, John F Reed, Scott L Mader
A significant finding with aging humans (and aging animal models) is that blood vessels lose their ability to respond to beta-adrenergic receptor stimuli. Therefore, they produce less cyclic adenosine monophosphate (cAMP) and have decreased vasorelaxation with advancing age. This change likely contributes to hypertension, insufficient blood flow, and atherosclerosis. Our goal was to develop a vascular smooth muscle cell culture model that replicates the molecular and biochemical changes observed in blood vessels with advancing age...
August 2011: In Vitro Cellular & Developmental Biology. Animal
Elisabeth Dupin
Melanocytes, the pigmented cells of the skin, and the glial Schwann cells lining peripheral nerves are developmentally derived from an early and transient ectodermal structure of the vertebrate embryo, the neural crest, which is also at the origin of multiple neural and non-neural cell types. Besides melanocytes and neural cells of the peripheral nervous system, the neural crest cells give rise to mesenchymal cell types in the head, which form most of the craniofacial skeleton, dermis, fat tissue and vascular musculo-connective components...
2011: Biologie Aujourd'hui
Jun Zhang, Congxin Huang, Pan Wu, Jing Yang, Tao Song, Yongjun Chen, Xinrong Fan, Ten Wang
The advent of stem cell therapy brings about the hope to restore the loss of cardiac pacemaker cells. However, it is largely unknown whether cardiac stem cells are able to differentiate into pacemaker cells. The purpose of this study was to determine whether the heart of large juvenile mammals contains cardiac stem cells (CSCs), which are suitable as seed cells for restoration of cardiac pacemaker cell. The c-kit(+) CSCs were isolated from one-month-old mongrel dogs. CSCs that we sorted were self-renewing, and they could proliferate by clonal expansion...
October 2010: Tohoku Journal of Experimental Medicine
Masakuni Tokunaga, Mei-Lan Liu, Toshio Nagai, Koji Iwanaga, Katsuhisa Matsuura, Toshinao Takahashi, Masato Kanda, Naomichi Kondo, Pin Wang, Atsuhiko T Naito, Issei Komuro
Implantation of various types of cells into the heart has been reported to be effective for heart failure, however, it is unknown what kinds of cells are most suitable for myocardial repair. To examine which types of cells are most effective, we injected cell-Puramatrix™ (PM) complex into the border area and overlaid the cell-PM patch on the myocardial infarction (MI) area. We compared cardiac morphology and function at 2 weeks after transplantation. Among clonal stem cell antigen-1 positive cardiac progenitors with PM (cSca-1/PM), bone marrow mononuclear cells with PM (BM/PM), skeletal myoblasts with PM (SM/PM), adipose tissue-derived mesenchymal cells with PM (AMC/PM), PM alone (PM), and non-treated MI group (MI), the infarct area of cSca-1/PM was smaller than that of BM/PM, SM/PM, PM and MI...
December 2010: Journal of Molecular and Cellular Cardiology
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