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Adipose-derived mesenchymal cells

Fawaz Abomaray, Sebastian Gidlöf, Bartosz Bezubik, Mikael Engman, Cecilia Götherström
Endometriosis is an inflammatory disease marked by ectopic growth of endometrial cells. Mesenchymal stromal cells (MSC) have immunosuppressive properties that have been suggested as a treatment for inflammatory diseases. Therefore, the aim herein was to examine effects of allogeneic MSC on endometriosis-derived cells in vitro as a potential therapy for endometriosis. MSC from allogeneic adipose tissue (Ad-MSC) and stromal cells from endometrium (ESCendo ) and endometriotic ovarian cysts (ESCcyst ) from women with endometriosis were isolated...
2018: Stem Cells International
Simona Ceccarelli, Cristina Nodale, Enrica Vescarelli, Paola Pontecorvi, Valeria Manganelli, Giovanni Casella, Maria Giuseppina Onesti, Maurizio Sorice, Ferdinando Romano, Antonio Angeloni, Cinzia Marchese
Adipogenesis is regulated by a complex network of molecules, including fibroblast growth factors. Keratinocyte growth factor (KGF) has been previously reported to promote proliferation on rat preadipocytes, although the expression of its specific receptor, FGFR2-IIIb/KGFR, is not actually detected in mesenchymal cells. Here, we demonstrate that human adipose-derived stem cells (ASCs) show increased expression of KGF during adipogenic differentiation, especially in the early steps. Moreover, KGF is able to induce transient activation of ERK and p38 MAPK pathways in these cells...
2018: Stem Cells International
Mazaher Gholipourmalekabadi, Alexander M Seifalian, Aleksandra M Urbanska, Mir Davood Omrani, John G Hardy, Zahra Madjd, Seyed Mahmoud Hashemi, Hossein Ghanbarian, Peiman Brouki Milan, Masoud Mozafari, Rui L Reis, Subhas C Kundu, Ali Samadikuchaksaraei
Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds...
March 12, 2018: Biomacromolecules
Marcus S Niepel, Fadi Almouhanna, Bhavya K Ekambaram, Matthias Menzel, Andreas Heilmann, Thomas Groth
BACKGROUND: Cells possess a specialized machinery through which they can sense physical as well as chemical alterations in their surrounding microenvironment that affect their cellular behavior. AIM: In this study, we aim to establish a polyelectrolyte multilayer system of 24 layers of poly-l-lysine and hyaluronic acid to control stem cell response after chemical cross-linking. METHODS AND RESULTS: The multilayer build-up process is monitored using different methods, which show that the studied polyelectrolyte multilayer system grows exponentially following the islands and islets theory...
February 1, 2018: International Journal of Artificial Organs
Yu Bin Lee, Eun Mi Kim, Hayeon Byun, Hyung-Kwan Chang, Kwanghee Jeong, Zachary M Aman, Yu Suk Choi, Jungyul Park, Heungsoo Shin
Numerous methods have been reported for the fabrication of 3D multi-cellular spheroids and their use in stem cell culture. Current methods typically relying on the self-assembly of trypsinized, suspended stem cells, however, show limitations with respect to cell viability, throughput, and accurate recapitulation of the natural microenvironment. In this study, we developed a new system for engineering cell spheroids by self-assembly of micro-scale monolayer of stem cells. We prepared synthetic hydrogels with the surface of chemically formed micropatterns (squares/circles with width/diameter of 200 μm) on which mesenchymal stem cells isolated from human nasal turbinate tissue (hTMSCs) were selectively attached and formed a monolayer...
March 1, 2018: Biomaterials
Ting Shen, Qing-Qing Zheng, Jiang Shen, Qiu-Shi Li, Xing-Hui Song, Hong-Bo Luo, Chao-Yang Hong, Ke Yao
Background: Corneal stromal cells (CSCs) are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells (ADSCs) have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear...
March 20, 2018: Chinese Medical Journal
M Duran, A C M Luzo, J G de Souza, W J Favaro, P Garcia, N Duran
Graphene and graphene oxide topography have an effect on the fate of stem cells such as adhesion, differentiation, and proliferation. This overview clearly shows that a new design and manipulation of associated graphene oxide stem cell culture platforms are of paramount importance as a focus in stem cell to tissue engineering applications. This overview also proposes that a film of graphene oxide is an efficient platform to modulate structure and function of multipotent mesenchymal stem/stromal cells (MSCs) and in special human adipose tissue derived mesenchymal stromal/stem cells (AT-MSCs)...
March 7, 2018: Current Molecular Medicine
F Louwen, A Ritter, N N Kreis, J Yuan
Obesity is associated with a variety of disorders including cardiovascular diseases, diabetes mellitus and cancer. Obesity changes the composition and structure of adipose tissue, linked to pro-inflammatory environment, endocrine/metabolic dysfunction, insulin resistance and oxidative stress. Adipose-derived mesenchymal stem cells (ASCs) have multiple functions like cell renewal, spontaneous repair and homeostasis in adipose tissue. In this review article, we have summarized the recent data highlighting that ASCs in obesity are defective in various functionalities and properties including differentiation, angiogenesis, motility, multipotent state, metabolism and immunomodulation...
March 8, 2018: Obesity Reviews: An Official Journal of the International Association for the Study of Obesity
Tatiane Garcia Stancker, Stella Souza Vieira, Andrey Jorge Serra, Rafael do Nascimento Lima, Regiane Dos Santos Feliciano, José Antônio Silva, Solange Almeida Dos Santos, Marcia Ataize Dos Santos Vieira, Maíra Cecília Brandão Simões, Ernesto Cesar Leal-Junior, Paulo de Tarso Camillo de Carvalho
This study aimed to determine whether photobiomodulation therapy (PBMT) could improve the bioavailability and chondroprotective benefits of mesenchymal stem cells injected into the knees of rats used as an experimental model of osteoarthritis (OA) as well as reduce the expression of matrix metalloproteinases (MMPs) and degradation of type II collagen (COL2-1) in the cartilage. Adipose-derived stem/stromal cells (ADSCs) were collected from three male Fischer 344 rats and characterized by flow cytometry. Fifty female Fischer 344 rats were distributed into five groups of 10 animals each...
March 8, 2018: Lasers in Medical Science
Tetsuya Ikemoto, Rui Feng, Mitsuo Shimada, Yu Saito, Shuichi Iwahashi, Yuji Morine, Satoru Imura
OBJECTIVES: We aimed to develop a simple protocol for deriving insulin-producing cells (IPCs) from adipose-derived mesenchymal stem cells (ADSCs). We established a 2-step creation method and an acceleration strategy with a histone deacetylase inhibitor that promoted a pro-endocrine pancreatic lineage. METHODS: We seeded ADSCs in 96-well dishes and cultured in Dulbecco's modified Eagle's medium/F12 medium containing 1% fetal bovine serum, 1% B27 supplement, 1% N2 supplement, 50-ng/mL human activin A, and 10-nM exendin-4 for step 1 of differentiation (7 days)...
April 2018: Pancreas
Shuang Zhang, Zhiqiang Zhu, Yufeng Wang, Shi Liu, Chenqiong Zhao, Weijun Guan, Yuhua Zhao
The role of mesenchymal stem cells (MSCs) in cellular therapy is well recognized in this work. MSCs have advantages of high proliferation, clone formation, multi-lineage differentiation and immunosuppression. Furthermore, adipose-resident MSCs (ADSCs) are extensively employed due to its advantages of abundant source, low cost and simple operation. Many researchers have emphasized the role of adipose-resident MSCs in the development of therapies for liver injury, but few attentions were paid on the use of induced functional hepatocytes...
March 7, 2018: Cytotechnology
Saverio La Francesca, Johnathon M Aho, Matthew R Barron, Ellen W Blanco, Sherif Soliman, Lena Kalenjian, Ariel D Hanson, Elisaveta Todorova, Matthew Marsh, KaLia Burnette, Harout DerSimonian, Robert D Odze, Dennis A Wigle
Treatment of esophageal disease can necessitate resection and reconstruction of the esophagus. Current reconstruction approaches are limited to utilization of an autologous conduit such as stomach, small bowel, or colon. A tissue engineered construct providing an alternative for esophageal replacement in circumferential, full thickness resection would have significant clinical applications. In the current study, we demonstrate that regeneration of esophageal tissue is feasible and reproducible in a large animal model using synthetic polyurethane electro-spun grafts seeded with autologous adipose-derived mesenchymal stem cells (aMSCs) and a disposable bioreactor...
March 7, 2018: Scientific Reports
Yumin Zhu, Yaping Wu, Jie Cheng, Qiong Wang, Zhongwu Li, Yanling Wang, Dongmiao Wang, Hua Wang, Weibing Zhang, Jinhai Ye, Hongbing Jiang, Lin Wang
BACKGROUND: Adipose-derived stem cells (ADSCs) are an attractive cell source for bone tissue engineering and have great potential for bone regeneration and defect repair. The transcriptional coactivator with PDZ-binding motif (TAZ) has been demonstrated to modulate osteogenic and adipogenic differentiation of mesenchymal stem cells. However, its roles during ADSC differentiation and therapeutic potentials for bone regeneration have as yet not been well established. METHODS: TAZ expression was measured during osteogenic differentiation of ADSCs in vitro...
March 7, 2018: Stem Cell Research & Therapy
Hiroshi Mikamo, Meizi Jiang, Mahito Noro, Yasuo Suzuki, Nobuyuki Hiruta, Hiroyuki Unoki-Kubota, Wolfgang J Schneider, Hideaki Bujo
The upregulation of brown or brown-like beige adipocytes is a potential strategy for the prevention or treatment of diabetes and coronary artery diseases in obese patients. Epicardial adipose tissue (EAT) differs significantly from subcutaneous fat tissue (SAT) in metabolic properties. To investigate properties of EAT further, thermogenesis gene expression was investigated in human autopsy and murine samples, and adipocytes differentiated from EAT mesenchymal cells. Subsequently, analyzed EAT volume alterations were observed to be associated with weight reduction in obese patients by imaging...
March 6, 2018: Molecular Medicine Reports
Mahmoud Hashemi Tabar, Mohammad Reza Tabandeh, Eskandar Moghimipour, Dian Dayer, Ata A Ghadiri, Elham Allah Bakhshi, Mahmoud Orazizadeh, Mohammad Ali Ghafari
Pancreatic and duodenal homeobox 1 (Pdx1) and Sonic hedgehog (Shh) are the key regulators of beta-cell function. In vitro experiments have shown that there is significant cooperation between Pdx1 and Shh with regard to the production and maintenance of insulin-producing cells (IPCs). In this study, the combined effect of Pdx1 overexpression and Shh manipulation on the function of adipose tissue-derived IPCs was determined. A eukaryotic expression vector ( Pdx1- pCDNA3.1(+)) was constructed and transfected into a Chinese hamster ovary (CHO) cell line...
March 2018: FEBS Open Bio
D Kuah, S Sivell, T Longworth, K James, A Guermazi, F Cicuttini, Y Wang, S Craig, G Comin, D Robinson, J Wilson
BACKGROUND: Cell therapies are being investigated as potential disease modifying treatment options for osteoarthritis (OA). Progenza (PRG) comprises in vitro expanded mesenchymal stem cells derived from human donor adipose tissue combined with cell culture supernatant. The primary objective of this first-in-human study was to evaluate the safety and tolerability of PRG. METHODS: We conducted a single centre, randomized, double-blind, placebo-controlled, single ascending dose study...
March 6, 2018: Journal of Translational Medicine
Baharak Abd Emami, Elena Mahmoudi, Mohammad Ali Shokrgozar, Mohammad Mehdi Dehghan, Saeed Farzad Mohajeri, Nooshin Haghighipour, Seyyed Hossein Marjanmehr, Mohammad Molazem, Susan Amin, Hossein Gholami
Myocardial infarction is one of the leading causes of death all over the world. Mesenchymal stem cells (MSCs) transplantation has shown a promising potential to recovery of ischemic heart disease due to their capability in differentiating into cardiac cells. However, various investigations have been performed to optimize the efficacy of cardiac cell therapy in recent years. Here, we sought to interrogate the effect of autologous transplantation of undifferentiated and predifferentiated adipose and bone marrow-derived MSCs in a rabbit model of myocardial infarction and also to investigate whether cardiac function could be improved by mechanically induced MSCs via equiaxial cyclic strain...
March 6, 2018: Artificial Organs
Akira Furuta, Tokunori Yamamoto, Taro Igarashi, Yasuyuki Suzuki, Shin Egawa, Naoki Yoshimura
INTRODUCTION AND HYPOTHESIS: We investigated the effects of bladder wall injection of mesenchymal stem cells (MSCs) on bladder tissues, function, and nociceptive behavior in a chemically induced interstitial cystitis-like rat model. METHODS: Chemical cystitis of female rats was induced by intravesical instillation of 0.1 N hydrochloride (HCl) once a week for 2 weeks. Bladders were harvested 1, 2, 3, and 4 weeks after the second application for histological examination...
March 6, 2018: International Urogynecology Journal
Sorour Ahmadyan, Mahboubeh Kabiri, Noushin Tasharofi, Simzar Hosseinzadeh, Mousa Kehtari, Athena Hajari Zadeh, Masoud Soleimani, Ali Farazmand, Hana Hanaee-Ahvaz
Stem cells' fate during in vitro differentiation is influenced by biophysicochemical cues. Osmotic stress has proved to enhance chondrocyte marker expression, however its potent negative impacts had never been surveyed. We questioned whether specific osmotic conditions, regarding the osmolyte agent, could benefit chondrogenesis while dampening undesired concomitant hypertrophy and inflammatory responses. To examine the potential side effects of hypertonicity, we assessed cell proliferation as well as chondrogenic and hypertrophic marker expression of human Adipose Derived-MSC after a two week induction in chondrogenic media with either NaCl or Sorbitol, as the osmolyte agent to reach a +100 mOsm hypertonic condition...
February 28, 2018: Cellular and Molecular Biology
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