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Adipose-derived mesenchymal cells

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https://www.readbyqxmd.com/read/29772738/angiogenesis-pet-tracer-uptake-68-ga-nodaga-e-crgdyk-%C3%A2-in-induced-myocardial-infarction-and-stromal-cell-treatment-in-minipigs
#1
Thomas Rasmussen, Bjarke Follin, Jens Kastrup, Malene Brandt-Larsen, Jacob Madsen, Thomas Emil Christensen, Morten Juhl, Smadar Cohen, Karsten Pharao Hammelev, Christian Holdflod Møller, Jens Peter Goetze, Philip Hasbak, Andreas Kjær
Angiogenesis is considered integral to the reparative process after ischemic injury. The αv β₃ integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68 Ga-NODAGA-E[(cRGDyK)]₂ (RGD) is a positron-emission-tomography (PET) ligand targeted towards αv β₃ integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI)...
May 16, 2018: Diagnostics
https://www.readbyqxmd.com/read/29770732/characterization-of-canine-adipose-derived-mesenchymal-stromal-stem-cells-in-serum-free-medium
#2
Zhuoming Liu, Rudell Screven, Lynne Boxer, Michael Myers, Lax Devireddy
In this manuscript, we report on the development of a defined serum-free medium capable of supporting the culture expansion of mesenchymal stromal/stem cells (MSCs) from canine adipose tissue (canine Ad-MSCs). The potential benefits of serum-free media can only be utilized if cells cultured in serum-free media maintain the same functional characteristics as cells cultured in serum-containing media. Therefore, we analyze the characteristics of canine Ad-MSCs cultured in this serum-free medium or in serum-containing medium through evaluation of growth kinetics, clonogenic capacity, senescence, and differentiation capacity...
May 17, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29768414/adipose-derived-mesenchymal-stem-cells-admsc-for-the-treatment-of-secondary-progressive-multiple-sclerosis-a-triple-blinded-placebo-controlled-randomized-phase-i-ii-safety-and-feasibility-study
#3
Oscar Fernández, Guillermo Izquierdo, Victoria Fernández, Laura Leyva, Virginia Reyes, Miguel Guerrero, Antonio León, Carlos Arnaiz, Guillermo Navarro, Maria Dolores Páramo, Antonio De la Cuesta, Bernat Soria, Abdelkrim Hmadcha, David Pozo, Rafael Fernandez-Montesinos, Maria Leal, Itziar Ochotorena, Patricia Gálvez, Maria Angeles Geniz, Francisco Javier Barón, Rosario Mata, Cristina Medina, Carlos Caparrós-Escudero, Ana Cardesa, Natividad Cuende
BACKGROUND: Currently available treatments for secondary progressive multiple sclerosis(SPMS) have limited efficacy and/or safety concerns. Adipose-mesenchymal derived stem cells(AdMSCs) represent a promising option and can be readily obtained using minimally invasive procedures. PATIENTS AND METHODS: In this triple-blind, placebo-controlled study, cell samples were obtained from consenting patients by lipectomy and subsequently expanded. Patients were randomized to a single infusion of placebo, low-dose(1x106cells/kg) or high-dose(4x106cells/kg) autologous AdMSC product and followed for 12 months...
2018: PloS One
https://www.readbyqxmd.com/read/29766931/-in-vitro-differentiation-of-neural-cells-from-human-adipose-tissue-derived-stromal-cells
#4
Shruti D Dave, Chetan N Patel, Aruna V Vanikar, Hargovind L Trivedi
Background: Stem cells, including neural stem cells (NSCs), are endowed with self-renewal capability and hence hold great opportunity for the institution of replacement/protective therapy. We propose a method for in vitro generation of stromal cells from human adipose tissue and their differentiation into neural cells. Materials and Methods: Ten grams of donor adipose tissue was surgically resected from the abdominal wall of the human donor after the participants' informed consents...
May 2018: Neurology India
https://www.readbyqxmd.com/read/29766824/adipose-derived-mesenchymal-stem-cells-in-the-treatment-of-obesity-a-systematic-review-of-longitudinal-studies-on-preclinical-evidence
#5
Fatima Saleh, Leila Itani, Simona Calugi, Riccardo Dalle Grave, Marwan El Ghoch
BACKGROUND: Obesity is an increasing global health problem, but its treatment is not yet optimal, especially in the long term. For this reason, preclinical studies have been conducted relating to a new therapeutic strategy for obesity based on adipose-derived mesenchymal stem cells (AD-MSCs). The aim of our systematic review is to summarize these findings deriving from the animal model in order to establish whether there is sufficient evidence to justify going forward to clinical studies...
May 15, 2018: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29766151/the-effect-of-supernatant-product-of-adipose-tissue-derived-mesenchymal-stem-cells-and-density-gradient-centrifugation-preparation-methods-on-pregnancy-in-intrauterine-insemination-cycles-an-rct
#6
Hoda Fazaeli, Faezeh Davoodi, Naser Kalhor, Reza Tabatabaii Qomi
Background: One of the most important involved factors in pregnancy occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters. Objective: To compare the effect of preparation methods in order to IUI, SPAS and density gradient centrifugation (DGC). Materials and Methods: This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle...
March 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29765427/adipose-derived-mesenchymal-stem-cells-in-the-use-of-cartilage-tissue-engineering-the-need-for-a-rapid-isolation-procedure
#7
REVIEW
Sam L Francis, Serena Duchi, Carmine Onofrillo, Claudia Di Bella, Peter F M Choong
Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24-48 hours...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29765417/zinc-promotes-adipose-derived-mesenchymal-stem-cell-proliferation-and-differentiation-towards-a-neuronal-fate
#8
Mi-Young Moon, Hyun Jung Kim, Bo Young Choi, Min Sohn, Tae Nyoung Chung, Sang Won Suh
Zinc is an essential element required for cell division, migration, and proliferation. Under zinc-deficient conditions, proliferation and differentiation of neural progenitors are significantly impaired. Adipose-derived mesenchymal stem cells (AD-MSCs) are multipotent stem cells that can differentiate into neurons. The aim of this study was to evaluate the effect of zinc on AD-MSC proliferation and differentiation. We initially examined the effect of zinc on stem cell proliferation at the undifferentiated stage...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29763932/microvesicles-from-human-adipose-tissue-derived-mesenchymal-stem-cells-as-a-new-protective-strategy-in-osteoarthritic-chondrocytes
#9
Miguel Tofiño-Vian, Maria Isabel Guillén, María Dolores Pérez Del Caz, Antonio Silvestre, María José Alcaraz
BACKGROUND/AIMS: Chronic inflammation contributes to cartilage degeneration during the progression of osteoarthritis (OA). Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. METHODS: AD-MSC were isolated by collagenase treatment from adipose tissue from healthy individuals subjected to abdominal lipectomy surgery...
May 9, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29760738/dental-mesenchymal-stem-stromal-cells-and-their-exosomes
#10
REVIEW
Peter Stanko, Ursula Altanerova, Jana Jakubechova, Vanda Repiska, Cestmir Altaner
Stem cells derived from human dental pulp tissue (DP-MSC) differ from the other mesenchymal stem cells prepared from bone marrow or adipose tissue due to their embryonic origin from the neural crest and are of special interest because of their neurotropic character. Furthermore, the therapeutic potential of DP-MSCs is realized through paracrine action of extracellularly released components, for which exosomes play an important role. In this review, we intend to explore the properties of these cells with an emphasis on exosomes...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29757934/comparison-of-synthetic-media-designed-for-expansion-of-adipose-derived-mesenchymal-stromal-cells
#11
Michelle Lensch, Angela Muise, Lisa White, Michael Badowski, David Harris
Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible...
May 14, 2018: Biomedicines
https://www.readbyqxmd.com/read/29755530/the-effect-of-age-on-the-regenerative-potential-of-human-eyelid-adipose-derived-stem-cells
#12
Mingqi Zhang, Zhuoshi Wang, Yan Zhao, Lirong Zhang, Ling Xu, Liu Cao, Wei He
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years ( n = 4), (2) >20 years, <45 years ( n = 5), and (3) >55 years ( n = 4)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29751848/differentiation-of-adipose-derived-stem-cells-into-schwann-cell-like-cells-through-intermittent-induction-potential-advantage-of-cellular-transient-memory-function
#13
Xun Sun, Yun Zhu, He-Yong Yin, Zhi-Yuan Guo, Feng Xu, Bo Xiao, Wen-Li Jiang, Wei-Min Guo, Hao-Ye Meng, Shi-Bi Lu, Yu Wang, Jiang Peng
BACKGROUND: Peripheral nerve injury (PNI) is a worldwide issue associated with severe social and economic burden. Autologous nerve grafting, the gold standard treatment for peripheral nerve defects, still has a number of technical limitations. Tissue engineering technology is a novel therapeutic strategy, and mesenchymal stromal cells (MSCs) are promising seed cells for nerve tissue engineering. However, the efficiency of traditional methods for inducing the differentiation of MSCs to Schwann cell-like cells (SCLCs) remains unsatisfactory...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29751779/essential-role-of-atp6ap2-enrichment-in-caveolae-lipid-raft-microdomains-for-the-induction-of-neuronal-differentiation-of-stem-cells
#14
Nehman Makdissy, Katia Haddad, Jeanne D'arc AlBacha, Diana Chaker, Bassel Ismail, Albert Azar, Ghada Oreibi, David Ayoub, Ibrahim Achkar, Didier Quilliot, Ziad Fajloun
BACKGROUND: The subcellular distribution of prorenin receptor and adaptor protein ATP6AP2 may affect neurogenesis. In this study, we hypothesized that ATP6AP2 expression and subcellular relocalization from caveolae/lipid raft microdomains (CLR-Ms) to intracellular sites may correlate with neuronal differentiation (Neu-Dif) of adipose-derived mesenchymal stem cells (ADSCs). METHODS: Human ADSCs isolated from 24 healthy donors and 24 patients with neurological disorders (ND) were cultured and induced for Neu-Dif...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29748664/nanovesicles-from-adipose-derived-mesenchymal-stem-cells-inhibit-t-lymphocyte-trafficking-and-ameliorate-chronic-experimental-autoimmune-encephalomyelitis
#15
Alessia Farinazzo, Stefano Angiari, Ermanna Turano, Edoardo Bistaffa, Silvia Dusi, Serena Ruggieri, Roberta Bonafede, Raffaella Mariotti, Gabriela Constantin, Bruno Bonetti
Cell based-therapies represent promising strategies for the treatment of neurological diseases. We have previously shown that adipose stem cells (ASC) ameliorate chronic experimental autoimmune encephalomyelitis (EAE). Recent evidence indicates that most ASC paracrine effects are mediated by extracellular vesicles, i.e. micro- and nanovesicles (MVs and NVs). We show that preventive intravenous administration of NVs isolated from ASC (ASC-NVs) before disease onset significantly reduces the severity of EAE and decreases spinal cord inflammation and demyelination, whereas therapeutic treatment with ASC-NVs does not ameliorate established EAE...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29747379/magnesium-deprivation-potentiates-human-mesenchymal-stem-cell-transcriptional-remodeling
#16
Azzurra Sargenti, Sara Castiglioni, Elena Olivi, Francesca Bianchi, Alessandra Cazzaniga, Giovanna Farruggia, Concettina Cappadone, Lucia Merolle, Emil Malucelli, Carlo Ventura, Jeanette A M Maier, Stefano Iotti
Magnesium plays a pivotal role in energy metabolism and in the control of cell growth. While magnesium deprivation clearly shapes the behavior of normal and neoplastic cells, little is known on the role of this element in cell differentiation. Here we show that magnesium deficiency increases the transcription of multipotency markers and tissue-specific transcription factors in human adipose-derived mesenchymal stem cells exposed to a mixture of natural molecules, i.e., hyaluronic, butyric and retinoid acids, which tunes differentiation...
May 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29747027/size-dependent-effects-of-graphene-oxide-on-the-osteogenesis-of-human-adipose-derived-mesenchymal-stem-cells
#17
Ee-Seul Kang, Inbeom Song, Da-Seul Kim, Ukjae Lee, Jang-Kyo Kim, Hyungbin Son, Junhong Min, Tae-Hyung Kim
Graphene derivatives are known to be suitable for biomedical applications, especially for stem cell-based therapies. Herein, we report the size effects of graphene oxide (GO) on differentiation of human adipose-derived mesenchymal stem cells (hADMSCs), using micro-sized (MGO) and nano-sized graphene oxide (NGO) sheets. The MGO and NGO sheets having lateral sizes of 1-10 μm and 100-300 nm, respectively, are coated on glass substrates by drop casting. The hADMSCs grown on the MGO-coated substrates show enhanced cell spreading and proliferation rate when compared with those of NGO counterpart, regardless of their densities...
April 27, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29744428/comparative-investigations-of-structure-and-properties-of-micro-arc-wollastonite-calcium-phosphate-coatings-on-titanium-and-zirconium-niobium-alloy
#18
M B Sedelnikova, E G Komarova, Yu P Sharkeev, T V Tolkacheva, I A Khlusov, L S Litvinova, K A Yurova, V V Shupletsova
Investigation results of micro-arc wollastonite-calcium phosphate (W-CaP) biocoatings on the pure titanium (Ti) and Zr-1wt.%Nb (Zr-1Nb) alloy were presented. The voltages of 150-300 V generate the micro-arc oxidation (MAO) process with the initial amplitude current of 150-550 A and 100-350 A for Ti and Zr-1Nb substrates, respectively. The identical dependencies of changes of the coating thickness, surface roughness and adhesion strength on the process voltage were revealed for the both substrates. The W-CaP coatings with the thickness of 10-11 μm were formed on Ti and Zr-1Nb under the low process voltage of 130-150 V...
September 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29737535/neural-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-induced-by-glial-cell-conditioned-media
#19
Debora Lo Furno, Giuliana Mannino, Rosario Giuffrida, Elisa Gili, Carlo Vancheri, Maria S Tarico, Rosario E Perrotta, Rosalia Pellitteri
Adipose-derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2-3 passages. They were then cultured in OEC-CM or SC-CM for 24 hr or 7 days...
May 8, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29737401/transplantation-of-adipose-tissue-derived-stem-cells-ameliorates-autoimmune-pathogenesis-in-mrl-lpr-mice-modulation-of-the-balance-between-th17-and-treg
#20
W Zhang, Y-L Feng, C-Y Pang, F-A Lu, Y-F Wang
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells characterized by immunomodulatory properties and are therefore considered a promising tool for the treatment of autoimmune diseases. In this study, we aimed to investigate whether transplantation of adipose tissue-derived stem cells (ADSCs) affects the autoimmune pathogenesis in MRL/lpr mice. METHODS: Fifteen 12-week-old MRL/lpr mice were randomly divided into three groups: ADSC, cyclophosphamide (CTX), and control groups, with five mice in each group...
May 8, 2018: Zeitschrift Für Rheumatologie
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