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bacteria adhesion and biofilm formation

Benoit Couvigny, Saulius Kulakauskas, Nicolas Pons, Benoit Quinquis, Anne-Laure Abraham, Thierry Meylheuc, Christine Delorme, Pierre Renault, Romain Briandet, Nicolas Lapaque, Eric Guédon
Biofilm formation is crucial for bacterial community development and host colonization by Streptococcus salivarius , a pioneer colonizer and commensal bacterium of the human gastrointestinal tract. This ability to form biofilms depends on bacterial adhesion to host surfaces, and on the intercellular aggregation contributing to biofilm cohesiveness. Many S. salivarius isolates auto-aggregate, an adhesion process mediated by cell surface proteins. To gain an insight into the genetic factors of S. salivarius that dictate host adhesion and biofilm formation, we developed a screening method, based on the differential sedimentation of bacteria in semi-liquid conditions according to their auto-aggregation capacity, which allowed us to identify twelve mutations affecting this auto-aggregation phenotype...
2018: Frontiers in Microbiology
Hitesh Pingle, Peng-Yuan Wang, Helmut Thissen, Peter Kingshott
Micro- and nanotopographies can interfere with bacteria attachment, however, the interplay existing between surface chemistry and topography remains unclear. Here, self-assembled spherical micrometer- silica and nanometer poly(methyl methacrylate) (PMMA)-sized particles are used to make binary colloidal crystal (BCC) topographical patterns to study bacterial attachment. A uniform surface chemistry of allylamine plasma polymer (AAMpp) is coated on the top of the BCCs to study only the topography effects. The uncoated and coated BCCs are exposed to Pseudomonas aeruginosa, and the surfaces and bacteria are characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and fluorescence microscopy...
February 27, 2018: Small
Beatrice Gutt, Qun Ren, Irmgard Hauser-Gerspach, Piotr Kardas, Stefan Stübinger, Monika Astasov-Frauenhoffer, Tuomas Waltimo
Periodontitis is a very common health problem caused by formation of pathogenic bacterial biofilm that triggers inflammation resulting in either reversible gingivitis or irreversible periodontal hard and soft tissue damages, leading to loss of teeth when left untreated. Commensal bacteria play an important role in oral health in many aspects. Mainly by colonizing oral tissues, they (i) contribute to maturation of immune response, and (ii) foreclose attachment of pathobiont and, therefore, prevent from infection...
2018: Frontiers in Microbiology
Antonio Nunes Filho, Michelle de Medeiros Aires, Danilo Cavalcante Braz, Ruth Hinrichs, Alexandre José Macedo, Clodomiro Alves
Bacterial adhesion on three different surfaces: untreated Ti, plasma nitriding, and plasma carbonitriding Ti substrates were investigated. The samples were placed in bacterial cultures of Pseudomonas aeruginosa to assess biofilm formation. The correlation between the amount of bacteria attached to the surface after a lapse of time with nanotopography and physicochemical properties was performed. TiN showed the highest capacity to avoid bacterial adhesion, while presenting intermediate roughness and wettability...
February 2018: Artificial Organs
Géssica A Costa, Fernanda C P Rossatto, Aline W Medeiros, Ana Paula F Correa, Adriano Brandelli, Ana Paula G Frazzon, Amanda DE S DA Motta
The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods...
February 1, 2018: Anais da Academia Brasileira de Ciências
Naiya B Patel, Jorge A Hinojosa, Meifang Zhu, Danielle M Robertson
Purpose: We have previously shown that invasive strains of Pseudomonas aeruginosa exploit the robust neutrophil response to form biofilms on contact lens surfaces and invade the corneal epithelium. The present study investigated the ability of multiple bacterial genera, all commonly recovered during contact lens-related infectious events, to adhere to and form biofilms on contact lens surfaces in the presence of neutrophils. Methods: Five reference strains from the American Type Culture Collection were used: P...
2018: Molecular Vision
Sumreen Hayat, Saima Muzammil, Muhammad Hidayat Rasool, Zonaira Nisar, Syed Zajif Hussain, Anjum Nasim Sabri, Saba Jamil
The aim of the current investigation was to determine antibacterial and antibiofilm potential of magnesium oxide (MgO) nanoparticles against antibiotic resistant clinical strains of bacteria. MgO nanoparticles were synthesized by wet chemical method, and were further characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Antibacterial activity was determined by broth microdilution and agar diffusion method. Bradford method was used to assess cellular protein leakage as a result of loss of membrane integrity...
February 6, 2018: Microbiology and Immunology
Noreen K Nkosana, Daniel J Czyzyk, Zarek S Siegel, Joy M Cote, Erika A Taylor
Gram-negative bacteria comprise the majority of microbes that cause infections that are resistant to pre-existing antibiotics. The complex cell wall architecture contributes to their ability to form biofilms, which are often implicated in hospital-acquired infections. Biofilms promote antibiotic resistance by enabling the bacteria to survive hostile environments such as UV radiation, pH shifts, and antibiotics. The outer membrane of Gram-negative bacteria contains lipopolysaccharide (LPS), which plays a role in adhesion to surfaces and formation of biofilms...
February 2, 2018: Bioorganic & Medicinal Chemistry Letters
Shivali Jindal, Sanjeev Anand, Lloyd Metzger, Jayendra Amamcharla
Flow of milk through the plate heat exchanger (PHE) results in denaturation of proteins, resulting in fouling. This also accelerates bacterial adhesion on the PHE surface, eventually leading to the development of biofilms. During prolonged processing, these biofilms result in shedding of bacteria and cross-contaminate the milk being processed, thereby limiting the duration of production runs. Altering the surface properties of PHE, such as surface energy and hydrophobicity, could be an effective approach to reduce biofouling...
February 2, 2018: Journal of Dairy Science
Luiza P S Cassiano, Talita M S Ventura, Cintia M S Silva, Aline L Leite, Ana C Magalhães, Juliano P Pessan, Marília Afonso Rabelo Buzalaf
This study detected changes in the protein profile of the acquired enamel pellicle (AEP) formed in vivo after rinsing with whole milk, fat-free milk, or water. Nine subjects in good oral condition took part in the study. The acquired pellicle was formed in the morning, for 120 min, after prophylaxis with pumice. Following this, the volunteers rinsed with 10 mL of whole milk, fat-free milk, or deionized water for 30 s, following a blinded crossover protocol. After 60 min, the pellicle was collected with filter paper soaked in 3% citric acid and processed for analysis by liquid chromatography-electrospray ionization tandem mass spectrometry...
January 27, 2018: Caries Research
Roberto E Bruna, María Victoria Molino, Martina Lazzaro, Javier F Mariscotti, Eleonora García Véscovi
PrtA is the major secreted metalloprotease of Serratia marcescens. Previous reports implicate PrtA in the pathogenic capacity of this bacteria. PrtA is also clinically used as a potent analgesic and anti-inflammatory drug and its catalytic properties attract industrial interest. Comparatively, there is scarce knowledge about the mechanisms that physiologically govern PrtA expression in Serratia In this work, we demonstrate that PrtA production is de-repressed when the bacterial growth temperature decreases from 37°C to 30°C...
January 29, 2018: Journal of Bacteriology
Si-Yu Wang, Xue-Fei Sun, Wen-Jing Gao, Yi-Fu Wang, Bei-Bei Jiang, Muhammad Zaheer Afzal, Chao Song, Shu-Guang Wang
Development of novel approaches for biofouling mitigation is of crucial importance for membrane-based technologies. d-amino acids (d-AAs) have been proposed as a potential strategy to mitigate biofouling. However, the effect of bacterial cell-wall properties and d-AAs type on biofouling mitigation remains unclear. This study assesses the effect of d-AAs type on membrane biofouling control, towards Gram positive (G+) and Gram negative (G-) bacteria. Three kinds of d-AAs were found to inhibit both G+ and G- bacterial attachment in short-term attachment and dead-end filtration experiments...
January 2, 2018: Colloids and Surfaces. B, Biointerfaces
Sinosh Skariyachan, Vaishnavi Sneha Sridhar, Swathi Packirisamy, Supreetha Toplar Kumargowda, Sneha Basavaraj Challapilli
Pseudomonas aeruginosa, a Gram-negative, rod-shaped bacterium causes widespread diseases in humans. This bacterium is frequently related to nosocomial infections such as pneumonia, urinary tract infections (UTIs) and bacteriaemia especially in immunocompromised patients. The current review focuses on the recent perspectives on biofilms formation by these bacteria. Biofilms are communities of microorganisms in which cells stick to each other and often adhere to a surface. These adherent cells are usually embedded within a self-produced matrix of extracellular polymeric substance (EPS)...
January 19, 2018: Folia Microbiologica
Prem Krishnan Raghupathi, Jerneja Zupančič, Asker Daniel Brejnrod, Samuel Jacquiod, Kurt Houf, Mette Burmølle, Nina Gunde-Cimerman, Søren J Sørensen
Extreme habitats are not only limited to natural environments, but also apply to man-made systems, for instance household appliances such as dishwashers. Limiting factors, such as high temperatures, high and low pH, high NaCl concentrations, presence of detergents and shear force from water during washing cycles define the microbial survival in this extreme system. Fungal and bacterial diversity in biofilms isolated from rubber seals of 24 different household dishwashers were investigated using next generation sequencing...
January 12, 2018: Applied and Environmental Microbiology
Liyun Wang, Robert Keatch, Qi Zhao, John A Wright, Clare E Bryant, Anna L Redmann, Eugene M Terentjev
Biofilm formation on abiotic surfaces in food and medical industry can cause severe contamination and infection, yet how biological and physical factors determine cellular architecture of early biofilms and bacterial behavior of the constituent cells remains largely unknown. In this study we examine the specific role of type-I fimbriae in nascent stages of biofilm formation and the response of micro-colonies to environmental flow shear at single-cell resolution. The results show that type-I fimbriae are not required for reversible adhesion from plankton, but critical for irreversible adhesion of Escherichia coli (E...
January 12, 2018: Applied and Environmental Microbiology
C Honey Chandran, T R Keerthi
The Indian honey bee-Apis cerana indica, which harbors an abundant and diverse range of lactic acid bacteria (LAB) in their gut with beneficial effects was used as the source for the isolation of LAB. In the present study, two LAB isolates from honey bee gut were selected primarily based on their phenotypic and selective biochemical characterization, further PCR and identified using 16S rRNA sequencing as Lactobacillus plantarum and were registered in National Centre for Biotechnology Information (NCBI) under accession number KX519413 and KX519414...
January 8, 2018: FEMS Microbiology Letters
Andreas Obermeier, Jochen Schneider, Norbert Harrasser, Jutta Tübel, Heinrich Mühlhofer, Dominik Pförringer, Constantin von Deimling, Peter Foehr, Barbara Kiefel, Christina Krämer, Axel Stemberger, Matthias Schieker, Rainer Burgkart, Rüdiger von Eisenhart-Rothe
BACKGROUND: Surgical sutures can promote migration of bacteria and thus start infections. Antiseptic coating of sutures may inhibit proliferation of adhered bacteria and avoid such complications. OBJECTIVES: This study investigated the inhibition of viable adhering bacteria on novel antimicrobially coated surgical sutures using chlorhexidine or octenidine, a critical factor for proliferation at the onset of local infections. The medical need, a rapid eradication of bacteria in wounds, can be fulfilled by a high antimicrobial efficacy during the first days after wound closure...
2018: PloS One
Husamettin Aygun, Murat Karamese, Cem Ozic, Fikret Uyar
AIM: Adherent-invasive Escherichia coli (AIEC) pathovar has been identified in intestinal mucosa of patients with Crohn's disease. Our aim was to compare the impact of sterile mucosal media (Muc-M) originated from different parts of the intestine on some pathogenic traits of AIEC LF82 strain. MATERIALS & METHODS: Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cell-infection experiments and biofilm-formation assays...
January 5, 2018: Future Microbiology
Jochen Schmid, Broder Rühmann, Volker Sieber, Lorena Romero-Jiménez, Juan Sanjuán, Daniel Pérez-Mendoza
Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors. They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation. Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host...
2018: Methods in Molecular Biology
Mehrnoush Sharifinejad, Narjes Shokatpour, Farshad Farnaghi, Zohreh Abedinyfar, Fahimeh Asadi Amoli, Farahnoosh Doustdar
BACKGROUND: Staphylococcus epidermidis is one of the common causes of bacterial keratitis and post-operation infections. One of the most important virulence factors of S. epidermidis is biofilm formation. Poly-N-acetylglucosamine (PNAG) production is critical for biofilm formation in S. epidermidis. The intercellular adhesion (ica) operon is formed by icaA, icaD, icaB, and icaC genes, which participate in the biosynthesis of PNAG. Strains of S. epidermidis from different infections show different genotypes in relation to adhesion operon genes...
December 26, 2017: Eye & Contact Lens
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