keyword
MENU ▼
Read by QxMD icon Read
search

bacteria adhesion and biofilm formation

keyword
https://www.readbyqxmd.com/read/28339597/compositional-differences-in-multi-species-biofilms-formed-on-various-orthodontic-adhesives
#1
Jung-Sub An, Kyungsun Kim, Soha Cho, Bum-Soon Lim, Sug-Joon Ahn
Objectives: To investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics. Methods: Multi-species biofilms using 13 bacterial species were grown on the surfaces of composite, compomer, and resin-modified glass-ionomer cement (RMGI). The changes in Streptococcus mutans (Sm), Streptococcus sobrinus (Ss), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and total bacteria were determined at day 1 (T1) and day 4 (T2) using real-time polymerase chain reaction...
January 25, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28325767/cohesive-properties-of-the-caulobacter-crescentus-holdfast-adhesin-are-regulated-by-a-novel-c-di-gmp-effector-protein
#2
Kathrin S Sprecher, Isabelle Hug, Jutta Nesper, Eva Potthoff, Mohamed-Ali Mahi, Matteo Sangermani, Volkhard Kaever, Torsten Schwede, Julia Vorholt, Urs Jenal
When encountering surfaces, many bacteria produce adhesins to facilitate their initial attachment and to irreversibly glue themselves to the solid substrate. A central molecule regulating the processes of this motile-sessile transition is the second messenger c-di-GMP, which stimulates the production of a variety of exopolysaccharide adhesins in different bacterial model organisms. In Caulobacter crescentus, c-di-GMP regulates the synthesis of the polar holdfast adhesin during the cell cycle, yet the molecular and cellular details of this control are currently unknown...
March 21, 2017: MBio
https://www.readbyqxmd.com/read/28325766/staphylococcal-%C3%AE-toxin-modulates-human-aortic-endothelial-cell-and-platelet-function-through-sphingomyelinase-and-biofilm-ligase-activities
#3
Alfa Herrera, Katarina Kulhankova, Vijay K Sonkar, Sanjana Dayal, Aloysius J Klingelhutz, Wilmara Salgado-Pabón, Patrick M Schlievert
Staphylococcus aureus causes many infections, such as skin and soft tissue, pneumonia, osteomyelitis, and infective endocarditis (IE). IE is an endovascular infection of native and prosthetic valves and the lining of the heart; it is characterized by the formation of cauliflower-like "vegetations" composed of fibrin, platelets, other host factors, bacteria, and bacterial products. β-Toxin is an S. aureus virulence factor that contributes to the microorganism's ability to cause IE. This cytolysin has two enzymatic activities: sphingomyelinase (SMase) and biofilm ligase...
March 21, 2017: MBio
https://www.readbyqxmd.com/read/28295372/surface-charge-modification-decreases-pseudomonas-aeruginosa-adherence-in-vitro-and-bacterial-persistence-in-an-in-vivo-implant-model
#4
W Katherine Kao, Patricia M Gagnon, Joseph P Vogel, Richard A Chole
OBJECTIVE: Chronic, persistent infections complicate otologic procedures utilizing implantable devices such as cochlear implants or tympanostomy tubes. These infections are thought to be due to the establishment of microbial biofilms on implant surfaces. To address this issue, we hypothesized that surface charge modification may inhibit the formation of Pseudomonas aeruginosa biofilms on implant surfaces in vitro and in vivo. STUDY DESIGN: We evaluated the effect of surface charge modification on bacterial biofilm formation by assessing the effect of the surface charge on bacterial adhesion in vitro and bacterial persistence in vivo...
March 14, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28291321/nanoengineered-superhydrophobic-surfaces-of-aluminum-with-extremely-low-bacterial-adhesivity
#5
Ferdi Hizal, Natthakan Rungraeng, Junghoon Lee, Soojin Jun, Henk J Busscher, Henny C Van der Mei, Chang-Hwan Choi
Bacterial adhesion and biofilm formation on surfaces are troublesome in many industrial processes. Here, nanoporous and nanopillared aluminum surfaces were engineered by anodizing and post-etching processes and made hydrophilic (using the inherent oxide layer) or hydrophobic (applying a Teflon coating) with the aim of discouraging bacterial adhesion. Adhesion of Staphylococcus aureus ATCC 12600 (Gram-positive, spherically-shaped) and Escherichia coli K-12 (Gram-negative, rod-shaped) was evaluated to the nanoengineered surfaces under both static and flow conditions (fluid shear rate of 37 1/s)...
March 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28289087/reoxidation-of-the-thiol-disulfide-oxidoreductase-mdba-by-a-bacterial-vitamin-k-epoxide-reductase-in-the-biofilm-forming-actinobacterium-actinomyces-oris
#6
Truc Thanh Luong, Melissa E Reardon-Robinson, Sara D Siegel, Hung Ton-That
Post-translocational protein folding in the Gram-positive biofilm-forming actinobacterium Actinomyces oris is mediated by a membrane-bound thiol-disulfide oxidoreductase named MdbA, which catalyzes oxidative folding of nascent polypeptides transported by the Sec translocon. Reoxidation of MdbA involves a bacterial Vitamin K Epoxide Reductase (VKOR)-like protein that contains four cysteine residues C93/C101 and C175/C178 with the latter forming a canonical CXXC thioredoxin-like motif; however, the mechanism of VKOR-mediated reoxidation of MdbA is not known...
March 13, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28280487/candida-albicans-mycofilms-support-staphylococcus-aureus-colonization-and-enhances-miconazole-resistance-in-dual-species-interactions
#7
Ryan Kean, Ranjith Rajendran, Jennifer Haggarty, Eleanor M Townsend, Bryn Short, Karl E Burgess, Sue Lang, Owain Millington, William G Mackay, Craig Williams, Gordon Ramage
Polymicrobial inter-kingdom biofilm infections represent a clinical management conundrum. The presence of co-isolation of bacteria and fungi complicates the ability to routinely administer single antimicrobial regimens, and synergy between the microorganisms influences infection severity. We therefore investigated the nosocomial pathogens Staphylococcus aureus and Candida albicans with respect to antimicrobial intervention. We characterized the interaction using biofilm assays and evaluated the effect of miconazole treatment using in vitro and in vivo assays...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28267427/prevention-of-biomaterial-infection-by-pre-operative-incubation-with-human-cells
#8
Ramón Pérez-Tanoira, Antti A Aarnisalo, Kari K Eklund, Xia Han, Antti Soininen, Veli-Matti Tiainen, Jaime Esteban, Teemu J Kinnari
BACKGROUND: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation. METHODS: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1 × 10(5) cells/mL), the materials were incubated for 4...
March 7, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28259957/photodynamic-inactivation-of-fibroblasts-and-inhibition-of-staphylococcus%C3%A2-epidermidis-adhesion-and-biofilm-formation-by-toluidine-blue-o
#9
Xin Li, Zizhong Liu, Houfang Liu, Xi Chen, Yue Liu, Honglue Tan
Treating skin and soft tissue infections of severe limb traumas can be challenging. Crucial concerns focus on inhibiting biofilm formation by antibiotic‑resistant bacteria, and preventing scar formation by fibroblastic hyperproliferation. The local use of toluidine blue O (TBO)‑mediated photodynamic therapy (PDT) may be a promising strategy for treating such lesions. The present study used Staphylococcus epidermidis (strain ATCC 35984) to assess the effects of TBO‑PDT on bacterial adherence and biofilm formation, using confocal laser scanning microscopy (CLSM), tissue culture plating (TCP) and scanning electron microscopy (SEM)...
February 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28243912/formation-of-biofilms-by-foodborne-pathogens-and-development-of-laboratory-in-vitro-model-for-the-study-of-campylobacter-genus-bacteria-based-on-these-biofilms
#10
N R Efimochkina, I B Bykova, Yu M Markova, Yu V Korotkevich, V V Stetsenko, L P Minaeva, S A Sheveleva
We analyzed the formation of biofilms by 7 strains of Campylobacter genus bacteria and 18 strains of Enterobacteriaceae genus bacteria that were isolated from plant and animal raw materials, from finished products, and swabs from the equipment of the food industry. Biofilm formation on glass plates, slides and coverslips, microtubes made of polymeric materials and Petri dishes, and polystyrene plates of different profiles were analyzed. When studying the process of films formation, different effects on bacterial populations were simulated, including variation of growth factor composition of culture media, technique of creating of anaerobiosis, and biocide treatment (active chlorine solutions in a concentration of 100 mg/dm(3))...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28237766/n-acetylcysteine-inhibits-growth-adhesion-and-biofilm-formation-of-gram-positive-skin-pathogens
#11
Daria Eroshenko, Tatyana Polyudova, Vladimir Korobov
N-Acetylcysteine (NAC) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of Gram-positive and Gram-negative bacteria. Our aim was to assess the effects of NAC on the growth of Gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. The minimum inhibitory concentrations (MICs) of NAC against Enterococcus faecalis, Corynebacterium ammoniagenes, Mycobacterium smegmatis, Propionibacterium acnes, Staphylococcus aureus, S. epidermidis, Streptococcus pyogenes, and 14 clinical strains of coagulase-negative staphylococci (CNS) ranged from 0...
February 22, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28221026/nanomechanics-of-ph-responsive-drug-loaded-bi-layered-polymer-grafts
#12
Prathima Chandra Nalam, Hyun-Su Lee, Nupur Bhatt, Robert W Carpick, David M Eckmann, Russell J Composto
Stimuli-responsive polymer films play an important role in the development of smart antibacterial coatings. In this study, we consider complementary architectures of polyelectrolyte films including a thin chitosan layer (CH), poly (acrylic acid) (PAA) brushes and a bilayer structure of CH grafted to PAA brushes (CH/PAA) as possible candidates for targeted drug delivery platforms. Atomic force microscopy (AFM) was employed to study the structure-mechanical property relationship for these mono- and bi- layered polymer grafts at pH 7...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28216386/serotonin-disturbs-colon-epithelial-tolerance-of-commensal-e-coli-by-increasing-nox2-derived-superoxide
#13
Suhrid Banskota, Sushil Chandra Regmi, Jaya Gautam, Pallavi Gurung, Yu-Jeong Lee, Sae Kwang Ku, Jin-Hyung Lee, Jintae Lee, Hyeun Wook Chang, Sang Joon Park, Jung-Ae Kim
Adherent-invasive E. coli colonization and Toll-like receptor (TLR) expression are increased in the gut of inflammatory bowel disease (IBD) patients. However, the underlying mechanism of such changes has not been determined. In the current study, it was examined whether gut serotonin (5-hydroxytryptamine, 5-HT) can induce adherent-invasive E. coli colonization and increase TLR expression. In a co-culture system, commensal E. coli strain (BW25113, BW) adhered minimally to colon epithelial cells, but this was significantly enhanced by 5-HT to the level of a pathogenic strain (EDL933)...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28213285/mechanical-and-chemical-studies-on-eps-from-sulfobacillus-thermosulfidooxidans-from-planktonic-to-biofilm-cells
#14
Qian Li, Wolfgang Sand
Bacteria attach to minerals and form biofilms, which can initiate and enhance bioleaching. Extracellular polymeric substances (EPS) play a crucial role during the whole process. Little is known how the cell surface/EPS mechanically and chemically respond to transformation from planktonic to biofilm cells. In this study the attachment and biofilm formation by Sulfobacillus thermosulfidooxidans were followed during pyrite leaching. Adhesiveness and stiffness of the cell/biofilm and the pyrite surface were checked by atomic force microscopy (AFM) in force mapping mode under real living conditions...
February 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28211364/antibacterial-effect-of-novel-biodegradable-and-bioresorbable-plda-mg-composites
#15
M C Fernández-Calderón, S C Cifuentes, M A Pacha-Olivenza, A M Gallardo-Moreno, L Saldaña, J L González-Carrasco, M T Blanco, N Vilaboa, M L González-Martín, C Pérez-Giraldo
Polylactic acid/Mg composites have been recently proposed for biodegradable osteosynthesis devices because, with regards to the neat polymer, they combine an enhanced biocompatibility and bioactivity with better mechanical properties, particularly creep strength. A question still arises about their bacterial behavior. For this purpose, composites of poly-L-D-lactic acid (PLDA) loaded with 1 and 10 wt.% of Mg microparticles were evaluated using Staphylococcus epidermidis, with special emphasis on the study of bacterial adhesion and biofilm formation...
February 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28186691/balancing-microbial-and-mammalian-cell-functions-on-calcium-ion-modified-implant-surfaces
#16
Eduardo Anitua, Ricardo Tejero, Miguel Ángel Pacha-Olivenza, María Coronada Fernández-Calderón, María Delgado-Rastrollo, Mari Mar Zalduendo, María Troya, Ciro Pérez-Giraldo, María Luisa González-Martín
Implant integration is a complex process mediated by the interaction of the implant surface with the surrounding ions, proteins, bacteria, and tissue cells. Although most implants achieve long-term bone-tissue integration, preventing pervasive implant-centered infections demands further advances, particularly in surfaces design. In this work, we analyzed classical microrough implant surfaces (only acid etched, AE; sandblasted then acid etching, SB + AE) and a new calcium-ion-modified implant surface (AE + Ca) in terms of soft- and hard-tissue integration, bacterial adhesion, and biofilm formation...
February 10, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28167523/the-role-of-cyclic-di-gmp-and-exopolysaccharide-in-type-iv-pilus-dynamics
#17
Jan Ribbe, Amy E Baker, Sebstian Euler, George A O'Toole, Berenike Maier
For Pseudomonas aeruginosa the levels of cyclic di-GMP govern the transition from the planktonic state to biofilm formation. Type IV pili (T4P) are crucial determinants of biofilm structure and dynamics, but it is unknown how the levels of c-di-GMP affect pilus dynamics. Here, we scrutinized how c-di-GMP affects molecular motor properties and adhesive behavior of T4P. By means of retraction, T4P generated forces of ∼ 30 pN. Deletion mutants in the proteins with known roles in biofilm formation, swarming motility and exopolysaccharide (EPS) production, specifically, the diguanulate cyclases sadC and roeA or the c-di-GMP phosphodiesterase bifA, showed only modest effects on velocity or force of T4P retraction...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28131941/rationally-designed-dual-functional-block-copolymers-for-bottlebrush-like-coatings-in-vitro-and-in-vivo-antimicrobial-antibiofilm-and-antifouling-properties
#18
Qiang Gao, Meng Yu, Yajuan Su, Meihua Xie, Xin Zhao, Peng Li, Peter X Ma
Numerous antimicrobial coatings have been developed for biomedical devices/implants, but few can simultaneously fulfill the requirements for antimicrobial and antifouling ability and biocompatibility. In this study, to develop an antimicrobial and antibiofilm surface coating, diblock amphiphilic molecules with antimicrobial and antifouling segments in a single chain were rationally designed and synthesized. Cationic antimicrobial polypeptides (AMP) were first synthesized by N-carboxyanhydride ring-opening polymerization (NCA-ROP)...
March 15, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28124027/bacterial-lysis-through-interference-with-peptidoglycan-synthesis-increases-biofilm-formation-by-nontypeable-haemophilus-influenzae
#19
Sara Marti, Carmen Puig, Alexandra Merlos, Miguel Viñas, Marien I de Jonge, Josefina Liñares, Carmen Ardanuy, Jeroen D Langereis
Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that mainly causes otitis media in children and community-acquired pneumonia or exacerbations of chronic obstructive pulmonary disease in adults. A large variety of studies suggest that biofilm formation by NTHi may be an important step in the pathogenesis of this bacterium. However, the underlying mechanisms involved in this process are poorly elucidated. In this study, we used a transposon mutant library to identify bacterial genes involved in biofilm formation...
January 2017: MSphere
https://www.readbyqxmd.com/read/28123297/a-decomposable-silica-based-antibacterial-coating-for-percutaneous-titanium-implant
#20
Jia Wang, Guofeng Wu, Xiangwei Liu, Guanyang Sun, Dehua Li, Hongbo Wei
Although percutaneous titanium implants have become one of the best choices as retainers in the facial defects, peri-implantitis still occurs at a significant rate. This unwanted complication occurs due to adhesion of bacteria and subsequent biofilm formation. To solve this problem, we have developed a novel antibiotic nanodelivery system based on self-decomposable silica nanoparticles. In this study, silica-gentamycin (SG) nanoparticles were successfully fabricated using an innovative one-pot solution. The nanoparticles were incorporated within a gelatin matrix and cross-linked on microarc-oxidized titanium...
2017: International Journal of Nanomedicine
keyword
keyword
116564
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"