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bacteria adhesion and biofilm formation

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https://www.readbyqxmd.com/read/28803472/blue-light-switchable-bacterial-adhesion-as-a-key-step-toward-the-design-of-biofilms
#1
Fei Chen, Seraphine V Wegner
The control of where and when bacteria adhere to a substrate is a key step toward controlling the formation and organization in biofilms. This study shows how we engineer bacteria to adhere specifically to substrates with high spatial and temporal control under blue light, but not in the dark, by using photoswitchable interaction between nMag and pMag proteins. For this, we express pMag proteins on the surface of E. coli so that the bacteria can adhere to substrates with immobilized nMag protein under blue light...
August 17, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28798735/phenotypic-heterogeneity-in-attachment-of-marine-bacteria-toward-antifouling-copolymers-unraveled-by-afm
#2
Sofiane El-Kirat-Chatel, Aurore Puymege, The H Duong, Perrine Van Overtvelt, Christine Bressy, Lénaïk Belec, Yves F Dufrêne, Maëlle Molmeret
Up to recent years, bacterial adhesion has mostly been evaluated at the population level. Single cell level has improved in the past few years allowing a better comprehension of the implication of individual behaviors as compared to the one of a whole community. A new approach using atomic force microscopy (AFM) to measure adhesion forces between a live bacterium attached via a silica microbead to the AFM tipless cantilever and the surface has been recently developed. The objectives of this study is to examine the bacterial adhesion to a surface dedicated to ship hulls at the population and the cellular level to understand to what extent these two levels could be correlated...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28792661/multi-effect-of-the-water-soluble-moringa-oleifera-lectin-against-serratia-marcescens-and-bacillus-sp-antibacterial-antibiofilm-and-anti-adhesive-properties
#3
M C Moura, D S Trentin, T H Napoleão, M Primon Barros, A S Xavier, N P Carneiro, P M G Paiva, A J Macedo, L C B B Coelho
AIMS: To evaluate the antibiofilm potential of water-soluble Moringa oleifera seed lectin (WSMoL) on Serratia marcescens and Bacillus sp. METHODS AND RESULTS: WSMoL inhibited biofilm formation by S. marcescens at concentrations lower than 2.6 μg ml(-1) and impaired bacterial growth at higher concentrations, avoiding biofilm formation. For Bacillus sp., the lectin inhibited bacterial growth at all concentrations. The antibiofilm action of WSMoL is associated with damage to bacterial cells...
August 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28777541/uv-curable-contact-active-benzophenone-terminated-quaternary-ammonium-antimicrobials-for-applications-in-polymer-plastics-and-related-devices
#4
Lukas Porosa, Alexander Caschera, Amanda Mocella, Joseph Bedard, Evan Ronan, Alan J Lough, Gideon Wolfaardt, Daniel Foucher
A series of UV active benzophenone ([C6H5COC6H4-O-(CH2)n-N+Me2R][X-], 4: R = C12H25, n = 3, X- = Br- , 5a-c: R = C18H37, n = 3, X- = Cl-, Br-, I-; 6a-c: R = C18H37, n = 4, X- = Cl-, Br-, I- ; 7a-c: R = C18H37, n = 6, X- = Cl-, Br-, I-) terminated C12 and C18 quaternary ammonium salts (QACs) were prepared by thermal or microwave driven Menshutkin protocols of the appropriate benzophenone alkyl halide (1a-c, 2a-c, 3a-c) with the corresponding dodecyl- or octadecyl N,N-dimethylamine. All new compounds were characterized by NMR spectroscopy, HRMS spectrometry and in one instance (4) by single crystal X-ray crystallography...
August 4, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28771505/a-mechanistic-individual-based-model-of-microbial-communities
#5
Pahala Gedara Jayathilake, Prashant Gupta, Bowen Li, Curtis Madsen, Oluwole Oyebamiji, Rebeca González-Cabaleiro, Steve Rushton, Ben Bridgens, David Swailes, Ben Allen, A Stephen McGough, Paolo Zuliani, Irina Dana Ofiteru, Darren Wilkinson, Jinju Chen, Tom Curtis
Accurate predictive modelling of the growth of microbial communities requires the credible representation of the interactions of biological, chemical and mechanical processes. However, although biological and chemical processes are represented in a number of Individual-based Models (IbMs) the interaction of growth and mechanics is limited. Conversely, there are mechanically sophisticated IbMs with only elementary biology and chemistry. This study focuses on addressing these limitations by developing a flexible IbM that can robustly combine the biological, chemical and physical processes that dictate the emergent properties of a wide range of bacterial communities...
2017: PloS One
https://www.readbyqxmd.com/read/28759643/in-vitro-comparison-between-%C3%AE-tocopheryl-acetate-and-%C3%AE-tocopheryl-phosphate-against-bacteria-responsible-of-prosthetic-and-joint-infections
#6
Alessandro Bidossi, Monica Bortolin, Marco Toscano, Elena De Vecchi, Carlo L Romanò, Roberto Mattina, Lorenzo Drago
Biofilm-related infections represent a recurrent problem in the orthopaedic setting. In recent years, great interest was directed towards the identification of novel molecules capable to interfere with pathogens adhesion and biofilm formation on implant surfaces. In this study, two stable forms of α-tocopherol, the hydrophobic acetate ester and the water-soluble phosphate ester, were tested in vitro as coating for titanium prosthesis. Antimicrobial activity against microorganisms responsible of prosthetic and joints infections was assessed by broth microdilution method...
2017: PloS One
https://www.readbyqxmd.com/read/28749324/investigation-of-the-fim1-putative-pilus-locus-of-streptococcus-equi-subspecies-equi
#7
Karen Frances Steward, Carl Robinson, Duncan J Maskell, Chiara Nenci, Andrew Stephen Waller
The Gram-positive bacterium Streptococcus equi subspecies equi (S. equi) is the causative agent of strangles, among the most frequently diagnosed infectious diseases of horses worldwide. Genome analysis of S. equi strain 4047 (Se4047) identified a putative operon, Fim1, with similarity to the pilus loci of other Gram-positive bacteria. The Fim1 locus was present in all strains of S. equi and its close relative S. equi subspecies zooepidemicus (S. zooepidemicus) that have been studied to date. In this study we provide evidence that the putative structural pilus proteins, SEQ_0936 and CNE, are produced on the cell surface during in vitro growth and in vivo infection...
July 28, 2017: Microbiology
https://www.readbyqxmd.com/read/28744274/tobramycin-and-amikacin-delay-adhesion-and-microcolony-formation-in-pseudomonas-aeruginosa-cystic-fibrosis-isolates
#8
Elodie Olivares, Stéphanie Badel-Berchoux, Christian Provot, Benoît Jaulhac, Gilles Prévost, Thierry Bernardi, François Jehl
Cystic fibrosis (CF) patients are predisposed to chronic colonization of the major airways by Pseudomonas aeruginosa biofilms. Pulmonary infections, involving sessile bacteria, are the main cause of morbidity and mortality. As the eradication of antibiotic-resistant biofilms remains impossible, one key objective for the treatment of lung infections is to delay the switch of P. aeruginosa to a sessile phenotype. Few tools are currently available in hospital laboratories to evaluate the susceptibility of adherent microorganisms to antimicrobials...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28704947/sponge-inspired-dibromohemibastadin-prevents-and-disrupts-bacterial-biofilms-without-toxicity
#9
Tiffany Le Norcy, Hendrik Niemann, Peter Proksch, Karen Tait, Isabelle Linossier, Karine Réhel, Claire Hellio, Fabienne Faÿ
Since the banning of several families of compounds in antifouling (AF) coatings, the search for environmentally friendly AF compounds has intensified. Natural sources of AF compounds have been identified in marine organisms and can be used to create analogues in laboratory. In a previous study, we identified that dibromohemibastadin-1 (DBHB) is a promising AF molecule, leading to the inhibition of the activity of phenoloxidase, an enzyme involved in the attachment of mussels to surfaces. This paper describes the activity of the DBHB on biofilm formation and its detachment and on bacterial adhesion and communication: quorum sensing...
July 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28703765/anti-biofilm-effect-of-biodegradable-coatings-based-on-hemibastadin-derivative-in-marine-environment
#10
Tiffany Le Norcy, Hendrik Niemann, Peter Proksch, Isabelle Linossier, Karine Vallée-Réhel, Claire Hellio, Fabienne Faÿ
Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed...
July 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28698836/copper-as-an-alternative-antimicrobial-coating-for-implants-an-in-vitro-study
#11
Claudia Bergemann, Sarah Zaatreh, Katharina Wegner, Kathleen Arndt, Andreas Podbielski, Rainer Bader, Cornelia Prinz, Ulrich Lembke, J Barbara Nebe
AIM: To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride (TiCuN) film and an additional BONIT(®) coating on titanium substrates. METHODS: For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray (TPS). On the TPS-coated samples, the TiCuN layer was deposited by physical vapor deposition. The BONIT(®) layer was coated electrochemically. The concentration of copper ions released from TiCuN films was measured by atomic absorption spectrometry...
June 24, 2017: World Journal of Transplantation
https://www.readbyqxmd.com/read/28697438/assessment-of-the-correlation-among-antibiotic-resistance-adherence-to-abiotic-and-biotic-surfaces-invasion-and-cytotoxicity-of-pseudomonas-aeruginosa-isolated-from-diseased-gilthead-sea-bream
#12
Faouzi Lamari, Ibtissem Chakroun, Sami Rtimi
Improper uses of antibiotics to treat fish disease pose an increase of multidrug resistance in Pseudomonas aeruginosa. In order to escape host antimicrobial agents and induce cytotoxicity, different virulence properties are needed by these bacteria such as, biofilm formation, adhesion and invasion ability. This study was conducted to isolate Pseudomonas aeruginosa from diseased cultured gilthead sea bream. Seventeen isolates of Pseudomonas aeruginosa were identified by PCR. All of the isolates tested were susceptible to Gentamicin and Ciprofloxacin...
July 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28695218/determination-of-the-nano-scaled-contact-area-of-staphylococcal-cells
#13
Christian Spengler, Nicolas Thewes, Philipp Jung, Markus Bischoff, Karin Jacobs
Bacterial adhesion is a crucial step during the development of infections as well as the formation of biofilms. Hence, fundamental research of bacterial adhesion mechanisms is of utmost importance. So far, less is known about the size of the contact area between bacterial cells and a surface. This gap will be filled by this study using a single-cell force spectroscopy-based method to investigate the contact area between a single bacterial cell of Staphylococcus aureus and a solid substrate. The technique relies on the strong influence of the hydrophobic interaction on bacterial adhesion: by incrementally crossing a very sharp hydrophobic/hydrophilic interface while performing force-distance curves with a single bacterial probe, the bacterial contact area can be determined...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28691240/superdurable-coating-fabricated-from-a-double-sided-tape-with-long-term-zero-bacterial-adhesion
#14
Wei Wang, Yang Lu, Hui Zhu, Zhiqiang Cao
There is no coating technology currently available to prevent the notorious biofilm formation issue. Here, a potential solution to fully address this tough issue is reported by developing a super-antifouling coating. The use of zwitterionic hydrogel (a double-sided tape) and commercial superglue is combined and a durable and ultrarobust antifouling zwitterionic (DURA-Z) coating is created that can be easily and universally applied on common substrates. Commercial superglue mostly for binding hydrophobic materials is used to strongly immobilize the superhydrophilic DURA-Z coating through interpenetration...
July 10, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28678862/pseudomonas-aeruginosa-cells-attached-to-a-surface-display-a-typical-proteome-early-as-20-minutes-of-incubation
#15
Marc Crouzet, Stéphane Claverol, Anne-Marie Lomenech, Caroline Le Sénéchal, Patricia Costaglioli, Christophe Barthe, Bertrand Garbay, Marc Bonneu, Sébastien Vilain
Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy...
2017: PloS One
https://www.readbyqxmd.com/read/28675109/titanium-surfaces-immobilized-with-the-major-antimicrobial-fragment-fk-16-of-human-cathelicidin-ll-37-are-potent-against-multiple-antibiotic-resistant-bacteria
#16
Biswajit Mishra, Guangshun Wang
Infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. Antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. Previously, the authors' laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17BIPHE2), a peptide engineered based on the only human cathelicidin. This study documents an alternative solution via covalent immobilization of FK-16, amino acid sequence FKRIVQRIKDFLRNLV-amide, which corresponds to the major antimicrobial region (residues 17-32) of LL-37...
July 4, 2017: Biofouling
https://www.readbyqxmd.com/read/28670572/biological-functions-of-the-secretome-of-neisseria-meningitidis
#17
REVIEW
Jan Tommassen, Jesús Arenas
Neisseria meningitidis is a Gram-negative bacterial pathogen that normally resides as a commensal in the human nasopharynx but occasionally causes disease with high mortality and morbidity. To interact with its environment, it transports many proteins across the outer membrane to the bacterial cell surface and into the extracellular medium for which it deploys the common and well-characterized autotransporter, two-partner and type I secretion mechanisms, as well as a recently discovered pathway for the surface exposure of lipoproteins...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28667623/shearing-and-enrichment-of-extracellular-type-iv-pili
#18
Alba Katiria Gonzalez Rivera, Katrina T Forest
Pili are widespread among bacteria. Type IVa pili (T4aP) are associated with a variety of bacterial functions, including adhesion, motility, natural transformation, biofilm formation, and force-dependent signaling. In pathogenic bacteria, T4aP play a crucial role during infection and have been the subject of hundreds of studies. Methods for the isolation and purification of T4aP were first described in the 1970s. Purified pili have been used for studies of filament protein content, morphology, immunogenicity, post-translational modifications, and X-ray crystallography...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28659548/pathogenicity-of-enterococci
#19
REVIEW
Yasuyoshi Ike
Enterococci belong to the group of lactic acid bacteria (LAB), and inhabit the gastrointestinal tracts of a wide variety of animals from insects and to human, and the commensal organism in humans and animals. The commensal/probiotic role of enterococci has evolved through thousands of years in mutual coexistence. Enterococcus have many favorable traits that have been appreciated in food fermentation and preservation, and many serve as probiotics to promote health. While lactobacillus have been shown to confer numerous benefits on and often regarded as health bringing organisms, enterococci have become more recognized as emerging human pathogens in recent years...
2017: Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology
https://www.readbyqxmd.com/read/28634241/psl-produced-by-mucoid-pseudomonas-aeruginosa-contributes-to-the-establishment-of-biofilms-and-immune-evasion
#20
Christopher J Jones, Daniel J Wozniak
Despite years of research and clinical advances, chronic pulmonary infections with mucoid Pseudomonas aeruginosa remain the primary concern for cystic fibrosis patients. Much of the research on these strains has focused on the contributions of the polysaccharide alginate; however, it is becoming evident that the neutral polysaccharide Psl also contributes to biofilm formation and the maintenance of chronic infections. Here, we demonstrate that Psl produced by mucoid strains has significant roles in biofilm structure and evasion of immune effectors...
June 20, 2017: MBio
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