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pathogenicity and biofilm formation

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https://www.readbyqxmd.com/read/28917089/the-trehalose-specific-transporter-lpqy-sugabc-is-required-for-antimicrobial-and-anti-biofilm-activity-of-trehalose-analogues-in-mycobacterium-smegmatis
#1
Jeffrey M Wolber, Bailey L Urbanek, Lisa M Meints, Brent F Piligian, Irene C Lopez-Casillas, Kailey M Zochowski, Peter J Woodruff, Benjamin M Swarts
Mycobacteria, including the bacterial pathogen that causes human tuberculosis, possess distinctive pathways for synthesizing and utilizing the non-mammalian disaccharide trehalose. Trehalose metabolism is essential for mycobacterial viability and has been linked to in vitro biofilm formation, which may bear relevance to in vivo drug tolerance. Previous research has shown that some trehalose analogues bearing modifications at the 6-position inhibit growth of various mycobacterial species. In this work, 2-, 5-, and 6-position-modified trehalose analogues were synthesized using our previously reported one-step chemoenzymatic method and shown to inhibit growth and biofilm formation in the two-to three-digit micromolar range in Mycobacterium smegmatis...
August 9, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28916560/cmeabc-multidrug-efflux-pump-contributes-to-antibiotic-resistance-and-promotes-campylobacter-jejuni-survival-and-multiplication-in-acanthamoeba-polyphaga
#2
Ana Vieira, Amritha Ramesh, Alan M Seddon, Andrey V Karlyshev
Campylobacter jejuni is a foodborne pathogen recognized as the leading cause of human bacterial gastroenteritis. The wide use of antibiotics in medicine and in animal husbandry has led to an increased incidence of antibiotic resistance in Campylobacter In addition to a role in multidrug resistance, the Campylobacter CmeABC RND-type efflux pump, which is associated with multidrug resistance (MDR), may also be involved in virulence. As a vehicle of pathogenic microorganisms, the protozoan Acanthamoeba is a good model for the investigation of bacterial survival in the environment and molecular mechanisms of pathogenicity...
September 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28913868/alterations-of-c-di-gmp-turnover-proteins-modulate-semi-constitutive-rdar-biofilm-formation-in-commensal-and-uropathogenic-escherichia-coli
#3
Annika Cimdins, Roger Simm, Fengyang Li, Petra Lüthje, Kaisa Thorell, Åsa Sjöling, Annelie Brauner, Ute Römling
Agar plate-based biofilm of enterobacteria like Escherichia coli is characterized by expression of the extracellular matrix components amyloid curli and cellulose exopolysaccharide, which can be visually enhanced upon addition of the dye Congo Red, resulting in a red, dry, and rough (rdar) colony morphology. Expression of the rdar morphotype depends on the transcriptional regulator CsgD and occurs predominantly at ambient temperature in model strains. In contrast, commensal and pathogenic isolates frequently express the csgD-dependent rdar morphotype semi-constitutively, also at human host body temperature...
September 15, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28912344/parasex-generates-phenotypic-diversity-de-novo-and-impacts-drug-resistance-and-virulence-in-candida-albicans
#4
Matthew P Hirakawa, Darius E Chyou, Denis Huang, Aaron R Slan, Richard J Bennett
Candida albicans is a diploid fungus that is a frequent cause of mucosal and systemic infections in humans. This species exhibits an unusual parasexual cycle in which mating produces tetraploid cells that undergo a non-meiotic program of concerted chromosome loss to return to a diploid or aneuploid state. In this work, we used a multipronged approach to examine the capacity of parasex to generate diversity in C. albicans First, we compared the phenotypic properties of 32 genotyped progeny and observed wide-ranging differences in fitness, filamentation, biofilm formation and virulence...
September 14, 2017: Genetics
https://www.readbyqxmd.com/read/28904973/effects-of-bacillus-serine-proteases-on-the-bacterial-biofilms
#5
Olga Mitrofanova, Ayslu Mardanova, Vladimir Evtugyn, Lydia Bogomolnaya, Margarita Sharipova
Serratia marcescens is an emerging opportunistic pathogen responsible for many hospital-acquired infections including catheter-associated bacteremia and urinary tract and respiratory tract infections. Biofilm formation is one of the mechanisms employed by S. marcescens to increase its virulence and pathogenicity. Here, we have investigated the main steps of the biofilm formation by S. marcescens SR 41-8000. It was found that the biofilm growth is stimulated by the nutrient-rich environment. The time-course experiments showed that S...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28904418/leaf-extracts-of-selected-gardening-trees-can-attenuate-quorum-sensing-and-pathogenicity-of-pseudomonas-aeruginosa-pao1
#6
Kaimin Niu, Min Kuk, Haein Jung, Kokgan Chan, Sooki Kim
An increasing concern on resistance to multiple-antibiotics has led to the discovery of novel agents and the establishment of new precaution strategy. Numerous plant sources have been widely studied to reduce virulence of pathogenic bacteria by interfering cell-to-cell based communication called quorum sensing (QS). Leaf extracts of 17 gardening trees were collected and investigated for their anti-QS effects using a sensor strain Chromobacterium violaceum CV026. Methanolic extracts of K4 (Acer palmatum), K9 (Acer pseudosieboldianum) and K13 (Cercis chinensis) leaves were selected for further experiments based on their antagonism effect on QS without inhibiting C...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28900419/enhanced-killing-and-antibiofilm-activity-of-encapsulated-cinnamaldehyde-against-candida-albicans
#7
Shahper N Khan, Shakir Khan, Jawed Iqbal, Rosina Khan, Asad U Khan
Candida sp. impelled opportunistic infection in immune-compromised patients ensuing from asymptomatic colonization to pathogenic forms. Moreover, slow spread of Candida species inducing refractory mucosal and invasive infections brings acute resistance to antifungal drugs. Hence, here we probed the effect of encapsulated preparation of cinnamaldehyde (CNMA) in multilamellar liposomes (ML) against Candida albicans. The efficacy of ML-CNMA against Candida biofilm was assessed by scanning electron microscopy, transmission electron microscopy, as well as light microscopy and its percent inhibition, was determined by XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] and crystal violet assay...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28900418/lmof2365_0442-encoding-for-a-fructose-specific-pts-permease-iia-may-be-required-for-virulence-in-l-monocytogenes-strain-f2365
#8
Yanhong Liu, Brian B Yoo, Cheng-An Hwang, Yujuan Suo, Shiowshuh Sheen, Parvaneh Khosravi, Lihan Huang
Listeria monocytogenes is a foodborne pathogen that causes listeriosis, which is a major public health concern due to the high fatality rate. LMOf2365_0442, 0443, and 0444 encode for fructose-specific EIIABC components of phosphotransferase transport system (PTS) permease that is responsible for sugar transport. In previous studies, in-frame deletion mutants of a putative fructose-specific PTS permease (LMOf2365_0442, 0443, and 0444) were constructed and analyzed. However, the virulence potential of these deletion mutants has not been studied...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28900144/structural-and-functional-insights-into-the-periplasmic-detector-domain-of-the-gacs-histidine-kinase-controlling-biofilm-formation-in-pseudomonas-aeruginosa
#9
Ahmad Ali-Ahmad, Firas Fadel, Corinne Sebban-Kreuzer, Moly Ba, Gauthier Dangla Pélissier, Olivier Bornet, Françoise Guerlesquin, Yves Bourne, Christophe Bordi, Florence Vincent
Pseudomonas aeruginosa is an opportunistic pathogenic bacterium responsible for both acute and chronic infections and has developed resistance mechanisms due to its ability to promote biofilm formation and evade host adaptive immune responses. Here, we investigate the functional role of the periplasmic detector domain (GacSPD) from the membrane-bound GacS histidine kinase, which is one of the key players for biofilm formation and coordination of bacterial lifestyles. A gacS mutant devoid of the periplasmic detector domain is severely defective in biofilm formation...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900103/yeej-is-an-inverse-autotransporter-from-escherichia-coli-that-binds-to-peptidoglycan-and-promotes-biofilm-formation
#10
Marta Martinez-Gil, Kelvin G K Goh, Elze Rackaityte, Chizuko Sakamoto, Bianca Audrain, Danilo G Moriel, Makrina Totsika, Jean-Marc Ghigo, Mark A Schembri, Christophe Beloin
Escherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT)...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900025/psp-stress-response-proteins-form-a-complex-with-mislocalized-secretins-in-the-yersinia-enterocolitica-cytoplasmic-membrane
#11
Disha Srivastava, Amal Moumene, Josué Flores-Kim, Andrew J Darwin
The bacterial phage shock protein system (Psp) is a conserved extracytoplasmic stress response that is essential for the virulence of some pathogens, including Yersinia enterocolitica It is induced by events that can compromise inner membrane (IM) integrity, including the mislocalization of outer membrane pore-forming proteins called secretins. In the absence of the Psp system, secretin mislocalization permeabilizes the IM and causes rapid cell death. The Psp proteins PspB and PspC form an integral IM complex with two independent roles...
September 12, 2017: MBio
https://www.readbyqxmd.com/read/28894437/evolution-of-stenotrophomonas-maltophilia-in-cystic-fibrosis-lung-over-chronic-infection-a-genomic-and-phenotypic-population-study
#12
Alfonso Esposito, Arianna Pompilio, Clotilde Bettua, Valentina Crocetta, Elisabetta Giacobazzi, Ersilia Fiscarelli, Olivier Jousson, Giovanni Di Bonaventura
Stenotrophomonas maltophilia has been recognized as an emerging multi-drug resistant opportunistic pathogen in cystic fibrosis (CF) patients. We report a comparative genomic and phenotypic analysis of 91 S. maltophilia strains from 10 CF patients over a 12-year period. Draft genome analyses included in silico Multi-Locus Sequence Typing (MLST), Single-Nucleotide Polymorphisms (SNPs), and pangenome characterization. Growth rate, biofilm formation, motility, mutation frequency, in vivo virulence, and in vitro antibiotic susceptibility were determined and compared with population structure over time...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28893915/hyperbiofilm-formation-by-bordetella-pertussis-strains-correlates-with-enhanced-virulence-traits
#13
Natalia Cattelan, Jamie Jennings-Gee, Purnima Dubey, Osvaldo M Yantorno, Rajendar Deora
Pertussis or whooping cough caused by the obligate human pathogen Bordetella pertussis is undergoing a world-wide resurgence. Majority of studies with this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in its pathogenesis. We conducted a side-by side comparison of the biofilm forming ability of the prototype laboratory strains with currently circulating isolates from two countries with different vaccination programs...
September 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28893789/gemini-cationic-amphiphiles-control-biofilm-formation-by-bacterial-vaginosis-pathogens
#14
Ammar Algburi, Yingyue Zhang, Richard Weeks, Nicole Comito, Saskia Zehm, Juanita Pinto, Kathryn E Uhrich, Michael L Chikindas
Antibiotic resistance and recurrence of bacterial vaginosis (BV), a polymicrobial infection, justify the need for novel antimicrobials to counteract microbial resistance to conventional antibiotics. Previously, two series of cationic amphiphiles (CAms), which self-assemble into supramolecular nanostructures with membrane-lytic properties, were designed with hydrophilic head groups and non-polar domains. The combination of CAms with commonly prescribed antibiotics is suggested as a promising strategy for targeting microorganisms that are resistant to conventional antibiotics...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28893777/modulation-of-staphylococcus-aureus-response-to-antimicrobials-by-the-candida-albicans-quorum-sensing-molecule-farnesol
#15
Eric F Kong, Christina Tsui, Sona Kucharíková, Patrick Van Dijck, Mary Ann Jabra-Rizk
In microbial biofilms, microorganisms utilize secreted signaling chemical molecules to coordinate their collective behavior. Farnesol is a quorum-sensing molecule secreted by the fungal species Candida albicans, shown to play a central physiological role during fungal biofilm growth. Our pervious in vitro and in vivo studies characterized an intricate interaction between C. albicans and the bacterial pathogen Staphylococcus aureus, as these species co-exist in biofilm. In this study, we aimed to investigate the impact of farnesol on S...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28893374/cell-differentiation-defines-acute-and-chronic-infection-cell-types-in-staphylococcus-aureus
#16
Juan-Carlos García-Betancur, Angel Goñi-Moreno, Thomas Horger, Melanie Schott, Malvika Sharan, Julian Eikmeier, Barbara Wohlmuth, Alma Zernecke, Knut Ohlsen, Christina Kuttler, Daniel Lopez
A central question to biology is how pathogenic bacteria initiate acute or chronic infections. Here we describe a genetic program for cell-fate decision in the opportunistic human pathogen Staphylococcus aureus, which generates the phenotypic bifurcation of the cells into two genetically identical but different cell types during the course of an infection. Whereas one cell type promotes the formation of biofilms that contribute to chronic infections, the second type is planktonic and produces the toxins that contribute to acute bacteremia...
September 12, 2017: ELife
https://www.readbyqxmd.com/read/28893370/inhibitory-efficacy-of-geraniol-on-biofilm-formation-and-development-of-adaptive-resistance-in-staphylococcus-epidermidis-rp62a
#17
Arunachalam Kannappan, Murugesan Sivaranjani, Ramanathan Srinivasan, Janarthanam Rathna, Shunmugiah Karutha Pandian, Arumugam Veera Ravi
PURPOSE: The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY: Biofilm biomass quantification assay was performed to evaluate the antibiofilm activity of GE against S. epidermidis. Microscopic observation of biofilms and extracellular polymeric substance (EPS), slime and cell surface hydrophobicity (CSH) production were also studied to support the antibiofilm potential of GE...
September 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28890886/development-of-a-novel-anti-adhesive-vaccine-against-pseudomonasaeruginosa-targeting-the-c-terminal-disulfide-loop-of-the-pilin-protein
#18
Sobhan Faezi, Ahmad Reza Bahrmand, Mehdi Mahdavi, Seyed Davar Siadat, Iraj Nikokar, Soroush Sardari
Type IV pili (T4P) are major virulence factors of Pseudomonas aeruginosa (P. aeruginosa) that are associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26b vector, which contains three domains, that each domain contains three tandem repeats. The flexible (GGGGS) and (GGGGS)3 linkers were linked between the three tandem repeats and each pilA domain, respectively...
2017: International Journal of Molecular and Cellular Medicine
https://www.readbyqxmd.com/read/28890436/primary-ciliary-dyskinesia-ciliated-airway%C3%A2-cells-show-increased-susceptibility-to-haemophilus-influenzae-biofilm-formation
#19
Woolf T Walker, Claire L Jackson, Raymond N Allan, Samuel A Collins, Michael J Kelso, Ardeshir Rineh, Nageshwar R Yepuri, Ben Nicholas, Laurie Lau, David Johnston, Peter Lackie, Saul N Faust, Jane S A Lucas, Luanne Hall-Stoodley
Non-typeable Haemophilus influenzae (NTHi) is the most common pathogen in primary ciliary dyskinesia (PCD) patients. We hypothesised that abnormal ciliary motility and low airway nitric oxide (NO) levels on airway epithelial cells from PCD patients might be permissive for NTHi colonisation and biofilm development.We used a primary epithelial cell co-culture model to investigate NTHi infection. Primary airway epithelial cells from PCD and non-PCD patients were differentiated to ciliation using an air-liquid interface culture and then co-cultured with NTHi...
September 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28890187/kinetic-analysis-of-the-inhibition-of-the-drug-efflux-protein-acrb-using-surface-plasmon-resonance
#20
Rumana Mowla, Yinhu Wang, Shutao Ma, Henrietta Venter
Multidrug efflux protein complexes such as AcrAB-TolC from Escherichia coli are paramount in multidrug resistance in Gram-negative bacteria and are also implicated in other processes such as virulence and biofilm formation. Hence efflux pump inhibition, as a means to reverse antimicrobial resistance in clinically relevant pathogens, has gained increased momentum over the past two decades. Significant advances in the structural and functional analysis of AcrB have informed the selection of efflux pump inhibitors (EPIs)...
September 7, 2017: Biochimica et Biophysica Acta
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