Wei-Syuan Huang, Yuan-Ju Lee, Lu Wang, Hsuan-Hsuan Chen, Yueh-Jung Chao, Vivien Cheng, Shwu-Jen Liaw
BACKGROUND: Copper plays a role in urinary tract infection (UTI) and urinary copper content is increased during Proteus mirabilis UTI. We therefore investigated the effect of copper on uropathogenic P. mirabilis and the underlying mechanisms, focusing on the virulence associated aspects. METHODS: Mouse colonization, swarming/swimming assays, measurement of cell length, flagellin level and urease activity, adhesion/invasion assay, biofilm formation, killing by macrophages, oxidative stress susceptibility, OMPs analysis, determination of MICs and persister cell formation, RT-PCR and transcriptional reporter assay were performed...
February 29, 2024: Journal of Microbiology Immunology and Infection