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outer membrane protein and biofilm formation

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https://www.readbyqxmd.com/read/27892689/biofilm-inhibitors-targeting-the-outer-membrane-protein-a-of-pasteurella-multocida-in-swine
#1
Anchanee Kubera, Arinthip Thamchaipenet, Menachem Shoham
Pasteurella multocida (Pm) is the causative agent of atrophic rhinitis in swine. This study aimed to discover biofilm inhibitors against swine Pm to counteract antibiotic resistance and decrease virulence. The virulence factor outer membrane protein A (OmpA) was targeted. A library of drugs approved by the Food and Drug Administration (FDA) was used to perform virtual screening against PmOmpA. The top-scoring compounds had no effect on the growth of Pm serotype A or D. Mycophenolate mofetil showed the highest efficacy in inhibiting biofilm formation by Pm serotype A, with an IC50 of 7...
November 28, 2016: Biofouling
https://www.readbyqxmd.com/read/27870886/comparative-genomics-revealed-multiple-helicobacter-pylori-genes-associated-with-biofilm-formation-in-vitro
#2
Eric Hong Jian Wong, Chow Goon Ng, Eng Guan Chua, Alfred Chin Yen Tay, Fanny Peters, Barry J Marshall, Bow Ho, Khean Lee Goh, Jamuna Vadivelu, Mun Fai Loke
BACKGROUND: Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. MATERIALS AND METHODS: Biofilm yield of 32 Helicobacter pylori strains (standard strain and 31 clinical strains) were determined by crystal-violet assay and grouped into poor, moderate and good biofilm forming groups. Whole genome sequencing of these 32 clinical strains was performed on the Illumina MiSeq platform...
2016: PloS One
https://www.readbyqxmd.com/read/27771071/differential-expression-of-putative-adhesin-genes-of-actinobacillus-suis-grown-in-in-vivo-like-conditions
#3
Adina R Bujold, Josée Labrie, Mario Jacques, Janet I MacInnes
Actinobacillus suis is an opportunistic pathogen that resides in the tonsils of the soft palate of swine. Unknown stimuli can cause this organism to invade the host, resulting in septicaemia and sequelae including death. To better understand its pathogenesis, the expression of several adhesin genes was evaluated by semi-quantitative real-time PCR in A. suis grown in conditions that mimic the host environment, including different nutrient and oxygen levels, exponential and stationary phases of growth, and in the presence of the stress hormone epinephrine...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27672084/impact-of-moderate-temperature-changes-on-neisseria-meningitidis-adhesive-phenotypes-and-proteome
#4
Martin Lappann, Andreas Otto, Madita Brauer, Dörte Becher, Ulrich Vogel, Kay Johswich
Neisseria meningitidis, the meningococcus, bears the potential of life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared meningococcal biofilm formation, autoaggregation and cellular adherence between 32°C and 37°C and found a clear increase in all these phenotypes at 32°C, suggestive for a stronger in vivo colonization capability at this temperature...
September 26, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27595778/role-of-ompa2-surface-regions-of-porphyromonas-gingivalis-in-host-pathogen-interactions-with-oral-epithelial-cells
#5
Kathryn L Naylor, Magdalena Widziolek, Stuart Hunt, Mary Conolly, Matthew Hicks, Prachi Stafford, Jan Potempa, Craig Murdoch, C W Ian Douglas, Graham P Stafford
Outer membrane protein A (OmpA) is a key outer membrane protein found in Gram-negative bacteria that contributes to several crucial processes in bacterial virulence. In Porphyromonas gingivalis, OmpA is predicted as a heterotrimer of OmpA1 and OmpA2 subunits encoded by adjacent genes. Here we describe the role of OmpA and its individual subunits in the interaction of P. gingivalis with oral cells. Using knockout mutagenesis, we show that OmpA2 plays a significant role in biofilm formation and interaction with human epithelial cells...
September 6, 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/27434141/conformational-dynamics-of-peb4-exhibit-mother-s-arms-chain-model-a-molecular-dynamics-study
#6
Sarath Chandra Dantu, Sagar Khavnekar, Avinash Kale
Peb4 from Campylobacter jejuni is an intertwined dimeric, periplasmic holdase, which also exhibits peptidyl prolyl cis/trans isomerase (PPIase) activity. Peb4 gene deletion alters the outer membrane protein profile and impairs cellular adhesion and biofilm formation for C. jejuni. Earlier crystallographic study has proposed that the PPIase domains are flexible and might form a cradle for holding the substrate and these aspects of Peb4 were explored using sub-microsecond molecular dynamics simulations in solution environment...
July 19, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27226289/xanthomonas-citri-ssp-citri-requires-the-outer-membrane-porin-oprb-for-maximal-virulence-and-biofilm-formation
#7
Florencia A Ficarra, Carolina Grandellis, Estela M Galván, Luis Ielpi, Regina Feil, John E Lunn, Natalia Gottig, Jorgelina Ottado
Xanthomonas citri ssp. citri (Xcc) causes canker disease in citrus, and biofilm formation is critical for the disease cycle. OprB (Outer membrane protein B) has been shown previously to be more abundant in Xcc biofilms compared with the planktonic state. In this work, we showed that the loss of OprB in an oprB mutant abolishes bacterial biofilm formation and adherence to the host, and also compromises virulence and efficient epiphytic survival of the bacteria. Moreover, the oprB mutant is impaired in bacterial stress resistance...
May 25, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27217419/htra-is-important-for-stress-resistance-and-virulence-in-haemophilus-parasuis
#8
Luhua Zhang, Ying Li, Yiping Wen, Gee W Lau, Xiaobo Huang, Rui Wu, Qigui Yan, Yong Huang, Qin Zhao, Xiaoping Ma, Xintian Wen, Sanjie Cao
Haemophilus parasuis is an opportunistic pathogen that causes Glässer's disease in swine, with polyserositis, meningitis, and arthritis. The high-temperature requirement A (HtrA)-like protease, which is involved in protein quality control, has been reported to be a virulence factor in many pathogens. In this study, we showed that HtrA of H. parasuis (HpHtrA) exhibited both chaperone and protease activities. Finally, nickel import ATP-binding protein (NikE), periplasmic dipeptide transport protein (DppA), and outer membrane protein A (OmpA) were identified as proteolytic substrates for HpHtrA...
August 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27216069/a-transposon-screen-identifies-genetic-determinants-of-vibrio-cholerae-resistance-to-high-molecular-weight-antibiotics
#9
Tobias Dörr, Fernanda Delgado, Benjamin D Umans, Matthew A Gerding, Brigid M Davis, Matthew K Waldor
Gram-negative bacteria are notoriously resistant to a variety of high-molecular-weight antibiotics due to the limited permeability of their outer membrane (OM). The basis of OM barrier function and the genetic factors required for its maintenance remain incompletely understood. Here, we employed transposon insertion sequencing to identify genes required for Vibrio cholerae resistance to vancomycin and bacitracin, antibiotics that are thought to be too large to efficiently penetrate the OM. The screen yielded several genes whose protein products are predicted to participate in processes important for OM barrier functions and for biofilm formation...
August 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27113583/structural-insights-into-the-regulatory-mechanism-of-the-pseudomonas-aeruginosa-yfibnr-system
#10
Min Xu, Xuan Yang, Xiu-An Yang, Lei Zhou, Tie-Zheng Liu, Zusen Fan, Tao Jiang
YfiBNR is a recently identified bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) signaling system in opportunistic pathogens. It is a key regulator of biofilm formation, which is correlated with prolonged persistence of infection and antibiotic drug resistance. In response to cell stress, YfiB in the outer membrane can sequester the periplasmic protein YfiR, releasing its inhibition of YfiN on the inner membrane and thus provoking the diguanylate cyclase activity of YfiN to induce c-di-GMP production. However, the detailed regulatory mechanism remains elusive...
June 2016: Protein & Cell
https://www.readbyqxmd.com/read/26965678/the-virulence-factor-ycho-has-a-pleiotropic-action-in-an-avian-pathogenic-escherichia-coli-apec-strain
#11
Livia Pilatti, Jacqueline Boldrin de Paiva, Thaís Cabrera Galvão Rojas, Janaína Luisa Leite, Rogério Arcuri Conceição, Gerson Nakazato, Wanderley Dias da Silveira
BACKGROUND: Avian pathogenic Escherichia coli strains cause extraintestinal diseases in birds, leading to substantial economic losses to the poultry industry worldwide. Bacteria that invade cells can overcome the host humoral immune response, resulting in a higher pathogenicity potential. Invasins are members of a large family of outer membrane proteins that allow pathogen invasion into host cells by interacting with specific receptors on the cell surface. RESULTS: An in silico analysis of the genome of a septicemic APEC strain (SEPT362) demonstrated the presence of a putative invasin homologous to the ychO gene from E...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/26957546/structural-basis-for-translocation-of-a-biofilm-supporting-exopolysaccharide-across-the-bacterial-outer-membrane
#12
Yan Wang, Archana Andole Pannuri, Dongchun Ni, Haizhen Zhou, Xiou Cao, Xiaomei Lu, Tony Romeo, Yihua Huang
The partially de-N-acetylated poly-β-1,6-N-acetyl-d-glucosamine (dPNAG) polymer serves as an intercellular biofilm adhesin that plays an essential role for the development and maintenance of integrity of biofilms of diverse bacterial species. Translocation of dPNAG across the bacterial outer membrane is mediated by a tetratricopeptide repeat-containing outer membrane protein, PgaA. To understand the molecular basis of dPNAG translocation, we determined the crystal structure of the C-terminal transmembrane domain of PgaA (residues 513-807)...
May 6, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26923129/indole-affects-the-formation-of-multicellular-aggregate-structures-in-pantoea-agglomerans-ys19
#13
Xuemei Yu, Jing Jiang, Chen Liang, Xiao Zhang, Jieru Wang, Delong Shen, Yongjun Feng
Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice. As well as having the ability to form a biofilm, as do most bacteria, it is characterized by the formation of a unique multicellular aggregate structure called symplasmata. Indole is traditionally known as a metabolite of the amino acid tryptophan, which, however, has recently been shown to participate in various regulations of bacterial physiological processes, including stress resistance, quorum sensing and biofilm formation...
2016: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/26851283/the-type-iv-pilus-assembly-atpase-pilb-of-myxococcus-xanthus-interacts-with-the-inner-membrane-platform-protein-pilc-and-the-nucleotide-binding-protein-pilm
#14
Lisa Franziska Bischof, Carmen Friedrich, Andrea Harms, Lotte Søgaard-Andersen, Chris van der Does
Type IV pili (T4P) are ubiquitous bacterial cell surface structures, involved in processes such as twitching motility, biofilm formation, bacteriophage infection, surface attachment, virulence, and natural transformation. T4P are assembled by machinery that can be divided into the outer membrane pore complex, the alignment complex that connects components in the inner and outer membrane, and the motor complex in the inner membrane and cytoplasm. Here, we characterize the inner membrane platform protein PilC, the cytosolic assembly ATPase PilB of the motor complex, and the cytosolic nucleotide-binding protein PilM of the alignment complex of the T4P machinery ofMyxococcus xanthus PilC was purified as a dimer and reconstituted into liposomes...
March 25, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26811318/small-rna-regulation-of-tolc-the-outer-membrane-component-of-bacterial-multidrug-transporters
#15
Ashley Parker, Susan Gottesman
UNLABELLED: Bacteria use multidrug efflux pumps to export drugs and toxic compounds out of the cell. One of the most important efflux pumps in Escherichia coli is the AcrAB-TolC system. Small regulatory RNAs (sRNAs) are known to be major posttranscriptional regulators that can enhance or repress translation by binding to the 5' untranslated region (UTR) of mRNA targets with the help of a chaperone protein, Hfq. In this study, we investigated the expression of acrA, acrB, and tolC translational fusions using 27 Hfq-dependent sRNAs overexpressed from plasmids...
January 25, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/26790928/outer-membrane-lipoprotein-vacj-is-required-for-the-membrane-integrity-serum-resistance-and-biofilm-formation-of-actinobacillus-pleuropneumoniae
#16
Fang Xie, Gang Li, Wanjiang Zhang, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang
The outer membrane proteins of Actinobacillus pleuropneumoniae are mediators of infection, acting as targets for the host's defense system. The outer membrane lipoprotein VacJ is involved in serum resistance and intercellular spreading in several pathogenic bacteria. To investigate the role of VacJ in the pathogenicity of Actinobacillus pleuropneumoniae, the vacJ gene-deletion mutant MD12 ΔvacJ was constructed. The increased susceptibility to KCl, SDS plus EDTA, and several antibiotics in the MD12ΔvacJ mutant suggested that the stability of the outer membrane was impaired as a result of the mutation in the vacJ gene...
February 1, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/26759990/outer-membrane-protein-a-plays-a-role-in-pathogenesis-of-acinetobacter-nosocomialis
#17
Sang Woo Kim, Man Hwan Oh, So Hyun Jun, Hyejin Jeon, Seung Il Kim, Kwangho Kim, Yoo Chul Lee, Je Chul Lee
Acinetobacter nosocomialis is an important nosocomial pathogen that causes a variety of human infections. However, the specific virulence factors of this microorganism have not yet been determined. We investigated the role of outer membrane protein A (OmpA) in the pathogenesis of A. nosocomialis. A ΔompA mutant of the A. nosocomialis ATCC 17903(T) strain was constructed using markerless gene deletion. The ΔompA mutant displayed reduced biofilm formation in polystyrene tubes and reduced adherence to A549 cells in comparison to the wild-type strain...
May 18, 2016: Virulence
https://www.readbyqxmd.com/read/26746604/a-proteomic-characterization-of-nthi-lysates
#18
Diego Preciado, Marian Poley, Stephanie Tsai, Amarel Tomney, Kristy Brown, Stephanie Val
BACKGROUND: Non-typeable Haemophilus influenzae (NTHi) is a ubiquitous bacterial pathogen which accounts for a majority of human upper respiratory tract infections. Laboratory lysate preparations from this bacterium are commonly utilized to investigate the promulgation of inflammatory responses in respiratory and middle ear epithelium both in vivo and in vitro. We undertook an unbiased proteomics based analysis of NTHi lysate preps to: (a) identify abundant bacterial proteins present in these lysates that could play a role in NTHi biological effects and (b) determine the protein content variability in different lysate prep batches from the same NTHI strain...
January 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/26673448/outer-membrane-protein-ompq-of-bordetella-bronchiseptica-is-required-for-mature-biofilm-formation
#19
Natalia Cattelan, María Inés Villalba, Gustavo Parisi, Laura Arnal, Diego Omar Serra, Mario Aguilar, Osvaldo Yantorno
Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B...
February 2016: Microbiology
https://www.readbyqxmd.com/read/26663346/the-versatile-tolc-like-slr1270-in-the-cyanobacterium-synechocystis-sp-pcc-6803
#20
Paulo Oliveira, Nuno M Martins, Marina Santos, Filipe Pinto, Zsófia Büttel, Narciso A S Couto, Phillip C Wright, Paula Tamagnini
Here we report on the functional characterization of the hypothetical protein Slr1270, a TolC homologue in Synechocystis sp. PCC 6803. Analysis of a slr1270 insertion deletion mutant and respective wild-type revealed that the mutant presents increased susceptibility to antibiotics. In addition, a detailed study of the exoproteome showed that Slr1270 mediates protein secretion. Among the protein substrates dependent on Slr1270 function, we found the S-layer structural component. Electron microscopy studies of the slr1270 mutant showed that the S-layer is indeed absent...
February 2016: Environmental Microbiology
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