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outer membrane protein and biofilm formation

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https://www.readbyqxmd.com/read/29594069/-tetrahymena-thermophila-predation-enhances-environmental-adaptation-of-the-carp-pathogenic-strain-aeromonas-hydrophila-nj-35
#1
Jin Liu, Yuhao Dong, Nannan Wang, Shougang Li, Yuanyuan Yang, Yao Wang, Furqan Awan, Chengping Lu, Yongjie Liu
Persistence of Aeromonas hydrophila in aquatic environments is the principle cause of fish hemorrhagic septicemia. Protistan predation has been considered to be a strong driving force for the evolution of bacterial defense strategies. In this study, we investigated the adaptive traits of A. hydrophila NJ-35, a carp pathogenic strain, in response to Tetrahymena thermophila predation. After subculturing with Tetrahymena , over 70% of A. hydrophila colonies were small colony variants (SCVs). The SCVs displayed enhanced biofilm formation, adhesion, fitness, and resistance to bacteriophage infection and oxidative stress as compared to the non- Tetrahymena- exposed strains...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29577084/aggregative-adherence-and-intestinal-colonization-by-enteroaggregative-escherichia-coli-are-produced-by-interactions-among-multiple-surface-factors
#2
Laura V Blanton, Lawrence T Wang, Jennifer Hofmann, Joshua DuBow, Alexander Lafrance, Stephen Kwak, Levi Bowers, Mandara A Levine, Charles O Hale, Philip M Meneely, Iruka N Okeke
Enteroaggregative Escherichia coli (EAEC) bacteria are exceptional colonizers that are associated with diarrhea. The genome of EAEC strain 042, a diarrheal pathogen validated in a human challenge study, encodes multiple colonization factors. Notable among them are aggregative adherence fimbriae (AAF/II) and a secreted antiaggregation protein (Aap). Deletion of aap is known to increase adherence, autoaggregation, and biofilm formation, so it was proposed that Aap counteracts AAF/II-mediated interactions. We hypothesized that Aap sterically masks heat-resistant agglutinin 1 (Hra1), an integral outer membrane protein recently identified as an accessory colonization factor...
March 2018: MSphere
https://www.readbyqxmd.com/read/29575515/conserved-structural-features-anchor-biofilm-associated-rtx-adhesins-to-the-outer-membrane-of-bacteria
#3
Shuaiqi Guo, David N Langelaan, Sean W Phippen, Steven P Smith, Ilja K Voets, Peter L Davies
Repeats-In-Toxin (RTX) adhesins are present in many Gram-negative bacteria to facilitate biofilm formation. Previously, we reported that the 1.5-MDa RTX adhesin (MpIBP) from the Antarctic bacterium, Marinomonas primoryensis, is tethered to the bacterial cell surface via its N-terminal Region I (RI). Here, we show the detailed structural features of RI. It has an N-terminal periplasmic retention domain (RIN), a central domain (RIM) that can insert into the β-barrel of an outer-membrane pore protein during MpIBP secretion, and three extracellular domains at its C terminus (RIC) that transition the protein into the extender region (RII)...
March 25, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29554105/sub-minimum-inhibitory-concentrations-of-colistin-and-polymyxin-b-promote-acinetobacter-baumannii-biofilm-formation
#4
Yoshinori Sato, Yuka Unno, Tsuneyuki Ubagai, Yasuo Ono
We investigated the numbers of planktonic and biofilm cells and the expression levels of genes encoding efflux pumps and biofilm-related proteins in 10 clinical isolates of multi-drug resistant Acinetobacter baumannii (MDRA) as well as in its standard strain ATCC 19606 in the presence of colistin (CST), polymyxin B (PMB), minomycin (MIN), and tigecycline (TGC) at their respective sub-MICs. The number of planktonic and biofilm cells of ATCC 19606 decreased in the presence of all aforementioned antibiotics in a dose-dependent manner...
2018: PloS One
https://www.readbyqxmd.com/read/29505819/the-role-of-tonb-dependent-copper-receptor-in-virulence-of-acinetobacter-baumannii
#5
Sajad Abdollahi, Iraj Rasooli, Seyed Latif Mousavi Gargari
Acinetobacter baumannii is an opportunistic gram negative pathogen that can adhere to different surfaces and cause different nosocomial infections. To investigate the role of TonB-dependent copper receptor, an outer membrane protein, in virulence of A. baumannii, we deleted this receptor from A. baumannii chromosome. There was a significant decrease in biofilm formation by copper receptor deficient mutant strain. Similarly, the adherence to human epithelial cell and the hydrophobicity were declined. The survival rate of the mutant strain in human sera was reduced while no change was observed in motility of strains...
March 6, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29484598/high-resolution-high-throughput-analysis-of-hfq-binding-sites-using-uv-crosslinking-and-analysis-of-cdna-crac
#6
Brandon Sy, Julia Wong, Sander Granneman, David Tollervey, David Gally, Jai J Tree
Small regulatory nonprotein-coding RNAs (sRNAs) have emerged as ubiquitous and abundant regulators of gene expression in a diverse cross section of bacteria. They play key roles in most aspects of bacterial physiology, including central metabolism, nutrient acquisition, virulence, biofilm formation, and outer membrane composition. RNA sequencing technologies have accelerated the identification of bacterial regulatory RNAs and are now being employed to understand their functions. Many regulatory RNAs require protein partners for activity, or modulate the activity of interacting proteins...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29480976/discovery-of-pantoea-stewartii-subsp-stewartii-genes-important-for-survival-in-corn-xylem-through-a-tn-seq-analysis
#7
Duy An Duong, Roderick V Jensen, Ann M Stevens
The bacterium Pantoea stewartii subsp. stewartii causes Stewart's wilt disease in corn. P. stewartii is transmitted to plants via corn flea beetles where it first colonizes the apoplast causing water-soaked lesions, then migrates to the xylem and forms a biofilm that blocks water transport. Bacterial quorum sensing insures the exopolysaccharide production necessary for biofilm formation occurs only at high cell density. A genomic-level Tn-Seq analysis was performed to identify additional bacterial genes essential for survival in planta and to provide insights into the plant-microbe interactions occurring during wilt disease...
February 26, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29477536/roles-of-outer-membrane-protein-w-ompw-on-survival-morphology-and-biofilm-formation-under-nacl-stresses-in-cronobacter-sakazakii
#8
Yingwang Ye, Na Ling, Jina Gao, Xiyan Zhang, Maofeng Zhang, Liaowang Tong, Haiyan Zeng, Jumei Zhang, Qingping Wu
Cronobacter sakazakii is an important foodborne pathogen associated with rare but severe infections through consumption of powdered infant formula. Tolerance to osmotic stress in Cronobacter has been described. However, the detailed factors involved in tolerance to osmotic stress in C. sakazakii are poorly understood. In this study, roles of outer membrane protein W (OmpW) on survival rates, morphologic changes of cells, and biofilm formation in C. sakazakii under different NaCl concentrations between wild type (WT) and OmpW mutant (ΔOmpW) were determined...
February 21, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29428742/short-communication-roles-of-outer-membrane-protein-w-ompw-on-survival-and-biofilm-formation-of-cronobacter-sakazakii-under-neomycin-sulfate-stress
#9
Yingwang Ye, Na Ling, Jina Gao, Maofeng Zhang, Xiyan Zhang, Liaowang Tong, Dexin Ou, Yaping Wang, Jumei Zhang, Qingping Wu
Cronobacter sakazakii is associated with severe infections including sepsis, neonatal meningitis, and necrotizing enterocolitis. Antibiotic resistance in Cronobacter species has been documented in recent years, but the genes involved in resistance in Cronobacter strains are poorly understood. In this study, we determined the role of outer membrane protein W (OmpW) on survival rates, morphologic changes, and biofilm formation between wild type (WT) and an OmpW mutant strain (ΔOmpW) under neomycin sulfate stress...
February 7, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29398539/synthesis-kinetics-and-inhibition-of-escherichia-coli-heptosyltransferase-i-by-monosaccharide-analogues-of-lipid-a
#10
Noreen K Nkosana, Daniel J Czyzyk, Zarek S Siegel, Joy M Cote, Erika A Taylor
Gram-negative bacteria comprise the majority of microbes that cause infections that are resistant to pre-existing antibiotics. The complex cell wall architecture contributes to their ability to form biofilms, which are often implicated in hospital-acquired infections. Biofilms promote antibiotic resistance by enabling the bacteria to survive hostile environments such as UV radiation, pH shifts, and antibiotics. The outer membrane of Gram-negative bacteria contains lipopolysaccharide (LPS), which plays a role in adhesion to surfaces and formation of biofilms...
February 2, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29229732/utilization-of-variant-and-fusion-proteins-to-functionally-map-the-aggregatibacter-actinomycetemcomitans-trimeric-autotransporter-protein-apia
#11
Carla Cugini, Yongyi Mei, David Furgang, Nisha George, Narayanan Ramasubbu, Daniel H Fine
The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is a causative agent of localized aggressive periodontitis. Critical to its infection process is the first and essential step of attachment, which is related to the coordinated functions of surface components comprised of proteins and extracellular polysaccharides. One such protein is the outer membrane trimeric autotransporter protein ApiA, a versatile virulence factor with numerous functions, including cell binding, invasion, serum resistance, autoaggregation, and induction of cytokine release...
March 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#12
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163446/more-than-gliding-involvement-of-gldd-and-gldg-in-the-virulence-of-flavobacterium-psychrophilum
#13
David Pérez-Pascual, Tatiana Rochat, Brigitte Kerouault, Esther Gómez, Fabienne Neulat-Ripoll, Celine Henry, Edwige Quillet, Jose A Guijarro, Jean F Bernardet, Eric Duchaud
A fascinating characteristic of most members of the genus Flavobacterium is their ability to move over surfaces by gliding motility. Flavobacterium psychrophilum , an important pathogen of farmed salmonids worldwide, contains in its genome the 19 gld and spr genes shown to be required for gliding or spreading in Flavobacterium johnsoniae ; however, their relative role in its lifestyle remains unknown. In order to address this issue, two spreading deficient mutants were produced as part of a Tn 4351 mutant library in F...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163381/functional-characterization-of-acinetobacter-baumannii-lacking-the-rna-chaperone-hfq
#14
Han-Yueh Kuo, Hsuan-Hao Chao, Po-Cheng Liao, Long Hsu, Kai-Chih Chang, Chi-Hua Tung, Chang-Hua Chen, Ming-Li Liou
The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq , A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29158242/mutations-in-sugar-nucleotide-synthesis-genes-restore-holdfast-polysaccharide-anchoring-to-caulobacter-crescentus-holdfast-anchor-mutants
#15
Gail G Hardy, Evelyn Toh, Cécile Berne, Yves V Brun
Attachment is essential for microorganisms to establish interactions with both biotic and abiotic surfaces. Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of the holdfast is unknown. The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB, and HfaD. <u>H</u>old<u>f</u>ast <u>a</u>nchor gene mutations result in holdfast shedding and reduced cell adherence. Translocation of HfaA and HfaD to the cell surface requires HfaB...
February 1, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29089624/combating-virulence-of-gram-negative-bacilli-by-ompa-inhibition
#16
Xavier Vila-Farrés, Raquel Parra-Millán, Viviana Sánchez-Encinales, Monica Varese, Rafael Ayerbe-Algaba, Nuria Bayó, Salvador Guardiola, María Eugenia Pachón-Ibáñez, Martin Kotev, Jesús García, Meritxell Teixidó, Jordi Vila, Jerónimo Pachón, Ernest Giralt, Younes Smani
Preventing the adhesion of pathogens to host cells provides an innovative approach to tackling multidrug-resistant bacteria. In this regard, the identification of outer membrane protein A (OmpA) as a key bacterial virulence factor has been a major breakthrough. The use of virtual screening helped us to identify a cyclic hexapeptide AOA-2 that inhibits the adhesion of Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli to host cells and the formation of biofilm, thereby preventing the development of infection in vitro and in a murine sepsis peritoneal model...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29075243/-acinetobacter-baumannii-virulence-traits-a-comparative-study-of-a-novel-sequence-type-with-other-italian-endemic-international-clones
#17
Cecilia Ambrosi, Daniela Scribano, Marta Aleandri, Carlo Zagaglia, Laura Di Francesco, Lorenza Putignani, Anna Teresa Palamara
Carbapenem-resistant Acinetobacter baumannii (CRAb) have emerged in recent decades as major causes of nosocomial infections. Resistance is mainly due to overexpression of intrinsic and/or acquired carbapenemases, especially oxacillinases (OXA). In Italy, although the sequence type (ST) 2 and the ST78 are the most frequently detected, we recently reported ST632, a single locus variant of ST2. Therefore, this study was aimed at unraveling common bacterial surface virulence factors involved in pathogenesis and antibiotic resistance in representative CRAb of these ST genotypes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29074368/planctomycetes-attached-to-algal-surfaces-insight-into-their-genomes
#18
Mafalda Faria, Nicola Bordin, Jana Kizina, Jens Harder, Damien Devos, Olga M Lage
Planctomycetes are bacteria with complex molecular and cellular biology. They have large genomes, some over 7Mb, and complex life cycles that include motile cells and sessile cells. Some live on the complex biofilm of macroalgae. Factors governing their life in this environment were investigated at the genomic level. We analyzed the genomes of three planctomycetes isolated from algal surfaces. The genomes were 6.6Mbp to 8.1Mbp large. Genes for outer-membrane proteins, peptidoglycan and lipopolysaccharide biosynthesis were present...
October 23, 2017: Genomics
https://www.readbyqxmd.com/read/29063141/cmpx-affects-virulence-in-pseudomonas-aeruginosa-through-the-gac-rsm-signaling-pathway-and-by-modulating-c-di-gmp-levels
#19
Anjali Y Bhagirath, Deepti Somayajula, Yanqi Li, Kangmin Duan
Pseudomonas aeruginosa is an ubiquitous organism which is able to infect and colonize many types of hosts including humans. Colonization of P. aeruginosa in chronic infections leads to the formation of biofilms, which are difficult to eradicate. P. aeruginosa is capable of regulating its virulence factors in response to external environment triggers and its signaling mechanism involves two-component regulatory systems and small molecules such as bis-(3'-5')-cyclic dimeric guanosine monophosphate. PA1611-RetS-GacS/A-RsmA/Y/Z is a key regulatory pathway in P...
October 23, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29040295/hydrophobic-residues-are-critical-for-the-helix-forming-hemolytic-and-bactericidal-activities-of-amphipathic-antimicrobial-peptide-tp4
#20
Ting-Wei Chang, Shu-Yi Wei, Shih-Han Wang, Hung-Mu Wei, Yu-June Wang, Chiu-Feng Wang, Chinpan Chen, You-Di Liao
Antimicrobial peptides are important components of the host innate defense mechanism against invading pathogens, especially for drug-resistant bacteria. In addition to bactericidal activity, the 25 residue peptide TP4 isolated from Nile tilapia also stimulates cell proliferation and regulates the innate immune system in mice. In this report, TP4 hyperpolarized and depolarized the membrane potential of Pseudomonas aeruginosa at sub-lethal and lethal concentrations. It also inhibited and eradicated biofilm formation...
2017: PloS One
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