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outer membrane protein and biofilm formation

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https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#1
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163446/more-than-gliding-involvement-of-gldd-and-gldg-in-the-virulence-of-flavobacterium-psychrophilum
#2
David Pérez-Pascual, Tatiana Rochat, Brigitte Kerouault, Esther Gómez, Fabienne Neulat-Ripoll, Celine Henry, Edwige Quillet, Jose A Guijarro, Jean F Bernardet, Eric Duchaud
A fascinating characteristic of most members of the genus Flavobacterium is their ability to move over surfaces by gliding motility. Flavobacterium psychrophilum, an important pathogen of farmed salmonids worldwide, contains in its genome the 19 gld and spr genes shown to be required for gliding or spreading in Flavobacterium johnsoniae; however, their relative role in its lifestyle remains unknown. In order to address this issue, two spreading deficient mutants were produced as part of a Tn4351 mutant library in F...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163381/functional-characterization-of-acinetobacter-baumannii-lacking-the-rna-chaperone-hfq
#3
Han-Yueh Kuo, Hsuan-Hao Chao, Po-Cheng Liao, Long Hsu, Kai-Chih Chang, Chi-Hua Tung, Chang-Hua Chen, Ming-Li Liou
The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq, A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29158242/mutations-in-sugar-nucleotide-synthesis-genes-restore-holdfast-polysaccharide-anchoring-to-caulobacter-crescentus-holdfast-anchor-mutants
#4
Gail G Hardy, Evelyn Toh, Cécile Berne, Yves V Brun
Attachment is essential for microorganisms to establish interactions with both biotic and abiotic surfaces. Stable attachment of Caulobacter crescentus to surfaces requires an adhesive polysaccharide holdfast, but the exact composition of holdfast is unknown. The holdfast is anchored to the cell envelope by outer membrane proteins HfaA, HfaB and HfaD. Holdfast anchor gene mutations result in holdfast shedding and reduced cell adherence. Translocation of HfaA and HfaD to the cell surface requires HfaB. The Wzx homolog, HfsF, is predicted to be a bacterial polysaccharide flippase...
November 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29089624/combating-virulence-of-gram-negative-bacilli-by-ompa-inhibition
#5
Xavier Vila-Farrés, Raquel Parra-Millán, Viviana Sánchez-Encinales, Monica Varese, Rafael Ayerbe-Algaba, Nuria Bayó, Salvador Guardiola, María Eugenia Pachón-Ibáñez, Martin Kotev, Jesús García, Meritxell Teixidó, Jordi Vila, Jerónimo Pachón, Ernest Giralt, Younes Smani
Preventing the adhesion of pathogens to host cells provides an innovative approach to tackling multidrug-resistant bacteria. In this regard, the identification of outer membrane protein A (OmpA) as a key bacterial virulence factor has been a major breakthrough. The use of virtual screening helped us to identify a cyclic hexapeptide AOA-2 that inhibits the adhesion of Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli to host cells and the formation of biofilm, thereby preventing the development of infection in vitro and in a murine sepsis peritoneal model...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29075243/acinetobacter-baumannii-virulence-traits-a-comparative-study-of-a-novel-sequence-type-with-other-italian-endemic-international-clones
#6
Cecilia Ambrosi, Daniela Scribano, Marta Aleandri, Carlo Zagaglia, Laura Di Francesco, Lorenza Putignani, Anna Teresa Palamara
Carbapenem-resistant Acinetobacter baumannii (CRAb) have emerged in recent decades as major causes of nosocomial infections. Resistance is mainly due to overexpression of intrinsic and/or acquired carbapenemases, especially oxacillinases (OXA). In Italy, although the sequence type (ST) 2 and the ST78 are the most frequently detected, we recently reported ST632, a single locus variant of ST2. Therefore, this study was aimed at unraveling common bacterial surface virulence factors involved in pathogenesis and antibiotic resistance in representative CRAb of these ST genotypes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29074368/planctomycetes-attached-to-algal-surfaces-insight-into-their-genomes
#7
Mafalda Faria, Nicola Bordin, Jana Kizina, Jens Harder, Damien Devos, Olga M Lage
Planctomycetes are bacteria with complex molecular and cellular biology. They have large genomes, some over 7Mb, and complex life cycles that include motile cells and sessile cells. Some live on the complex biofilm of macroalgae. Factors governing their life in this environment were investigated at the genomic level. We analyzed the genomes of three planctomycetes isolated from algal surfaces. The genomes were 6.6Mbp to 8.1Mbp large. Genes for outer-membrane proteins, peptidoglycan and lipopolysaccharide biosynthesis were present...
October 23, 2017: Genomics
https://www.readbyqxmd.com/read/29063141/cmpx-affects-virulence-in-pseudomonas-aeruginosa-through-the-gac-rsm-signaling-pathway-and-by-modulating-c-di-gmp-levels
#8
Anjali Y Bhagirath, Deepti Somayajula, Yanqi Li, Kangmin Duan
Pseudomonas aeruginosa is an ubiquitous organism which is able to infect and colonize many types of hosts including humans. Colonization of P. aeruginosa in chronic infections leads to the formation of biofilms, which are difficult to eradicate. P. aeruginosa is capable of regulating its virulence factors in response to external environment triggers and its signaling mechanism involves two-component regulatory systems and small molecules such as bis-(3'-5')-cyclic dimeric guanosine monophosphate. PA1611-RetS-GacS/A-RsmA/Y/Z is a key regulatory pathway in P...
October 23, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29040295/hydrophobic-residues-are-critical-for-the-helix-forming-hemolytic-and-bactericidal-activities-of-amphipathic-antimicrobial-peptide-tp4
#9
Ting-Wei Chang, Shu-Yi Wei, Shih-Han Wang, Hung-Mu Wei, Yu-June Wang, Chiu-Feng Wang, Chinpan Chen, You-Di Liao
Antimicrobial peptides are important components of the host innate defense mechanism against invading pathogens, especially for drug-resistant bacteria. In addition to bactericidal activity, the 25 residue peptide TP4 isolated from Nile tilapia also stimulates cell proliferation and regulates the innate immune system in mice. In this report, TP4 hyperpolarized and depolarized the membrane potential of Pseudomonas aeruginosa at sub-lethal and lethal concentrations. It also inhibited and eradicated biofilm formation...
2017: PloS One
https://www.readbyqxmd.com/read/29021252/a-chemical-and-biological-toolbox-for-type-vd-secretion-characterization-of-the-phospholipase-a1-autotransporter-fpla-from-fusobacterium-nucleatum
#10
Michael A Casasanta, Christopher C Yoo, Hans B Smith, Alison J Duncan, Kyla Cochrane, Ann C Varano, Emma Allen-Vercoe, Daniel J Slade
Fusobacterium nucleatum is an oral pathogen that is linked to multiple human infections and colorectal cancer. Strikingly, F. nucleatum achieves virulence in the absence of large, multi-protein secretion systems (Type I, II, III, IV, and VI) which are widely used by Gram-negative bacteria for pathogenesis. By contrast, F. nucleatum strains contain genomic expansions of Type V secreted effectors (autotransporters) that are critical for host cell adherence, invasion, and biofilm formation. Here we present the first characterization of a F...
October 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28972168/pela-and-pelb-form-a-modification-and-secretion-complex-essential-for-pel-polysaccharide-dependent-biofilm-formation-in-pseudomonas-aeruginosa
#11
Lindsey S Marmont, Gregory B Whitfield, Jacquelyn D Rich, Patrick Yip, Laura B Giesbrecht, Carol A Stremick, John C Whitney, Matthew R Parsek, Joe J Harrison, P Lynne Howell
The Pel polysaccharide (PEL) is synthesized by the opportunistic pathogen Pseudomonas aeruginosa and is an important biofilm constituent critical for bacterial virulence and persistence. PEL is a cationic polymer that promotes cell-cell interactions within the biofilm matrix through electrostatic interactions with extracellular DNA (eDNA). Translocation of PEL across the outer membrane is proposed to occur via PelB, a membrane-embedded porin with a large periplasmic domain predicted to contain 19 tetratricopeptide repeats (TPRs)...
September 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28959742/the-biofilm-inhibitor-carolacton-enters-gram-negative-cells-studies-using-a-tolc-deficient-strain-of-escherichia-coli
#12
Jannik Donner, Michael Reck, Boyke Bunk, Michael Jarek, Constantin Benjamin App, Jan P Meier-Kolthoff, Jörg Overmann, Rolf Müller, Andreas Kirschning, Irene Wagner-Döbler
The myxobacterial secondary metabolite carolacton inhibits growth of Streptococcus pneumoniae and kills biofilm cells of the caries- and endocarditis-associated pathogen Streptococcus mutans at nanomolar concentrations. Here, we studied the response to carolacton of an Escherichia coli strain that lacked the outer membrane protein TolC. Whole-genome sequencing of the laboratory E. coli strain TolC revealed the integration of an insertion element, IS5, at the tolC locus and a close phylogenetic relationship to the ancient E...
September 2017: MSphere
https://www.readbyqxmd.com/read/28900103/yeej-is-an-inverse-autotransporter-from-escherichia-coli-that-binds-to-peptidoglycan-and-promotes-biofilm-formation
#13
Marta Martinez-Gil, Kelvin G K Goh, Elze Rackaityte, Chizuko Sakamoto, Bianca Audrain, Danilo G Moriel, Makrina Totsika, Jean-Marc Ghigo, Mark A Schembri, Christophe Beloin
Escherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT)...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28900025/psp-stress-response-proteins-form-a-complex-with-mislocalized-secretins-in-the-yersinia-enterocolitica-cytoplasmic-membrane
#14
Disha Srivastava, Amal Moumene, Josué Flores-Kim, Andrew J Darwin
The bacterial phage shock protein system (Psp) is a conserved extracytoplasmic stress response that is essential for the virulence of some pathogens, including Yersinia enterocolitica It is induced by events that can compromise inner membrane (IM) integrity, including the mislocalization of outer membrane pore-forming proteins called secretins. In the absence of the Psp system, secretin mislocalization permeabilizes the IM and causes rapid cell death. The Psp proteins PspB and PspC form an integral IM complex with two independent roles...
September 12, 2017: MBio
https://www.readbyqxmd.com/read/28870806/structural-insights-into-the-functional-role-of-gmp-in-modulating-the-yfibnr-system
#15
Lei Zhou, Min Xu, Tao Jiang
YfiBNR, a tripartite cyclic-di-GMP (c-di-GMP) signalling system, plays an important role in biofilm formation of the gram-negative bacterium P. aeruginosa, which regulates the cellular processes strongly associated with chronic lung infections and drug resistance. The outer-membrane lipoprotein YfiB can release the inhibition of the inner membrane protein YfiN by sequestering the periplasmatic protein YfiR, resulting in the activation of diguanylate cyclase activity of YfiN and the production of c-di-GMP. In contrast to the extensive studies on c-di-GMP, little is known about how GMP acts in the YfiBNR system...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28859312/phase-variation-of-dna-methyltransferases-and-the-regulation-of-virulence-and-immune-evasion-in-the-pathogenic-neisseria
#16
Kate L Seib, Freda E-C Jen, Adeana L Scott, Aimee Tan, Michael P Jennings
The pathogenic Neisseria provide textbook examples of phase variation: the high frequency, random and reversible switching of gene expression. Typically, phase variable gene expression is observed in genes required for the expression of surface proteins and carbohydrate structures. All Neisseria gonorrhoeae and N. meningitidis strains also express phase variable DNA methyltransferases that are components of DNA restriction-modification systems. Phase variation of these DNA methyltransferases (Mod) alters global DNA methylation patterns...
August 31, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28800709/the-major-outer-membrane-protein-mopb-is-required-for-twitching-movement-and-affects-biofilm-formation-and-virulence-in-two-xylella-fastidiosa-strains
#17
Hongyu Chen, Prem P Kandel, Luisa F Cruz, Paul A Cobine, Leonardo De La Fuente
MopB is a major outer membrane protein (OMP) in Xylella fastidiosa, a bacterial plant pathogen that causes losses on many economically important crops. Based on in silico analysis, the uncharacterized MopB protein of X. fastidiosa contains a β-barrel structure with an OmpA-like domain and a predicted calcium-binding motif. Here, MopB function was studied by mutational analysis taking advantage of the natural competence of X. fastidiosa. Mutants of mopB were constructed in two different X. fastidiosa strains, the type strain Temecula and the more virulent WM1-1...
November 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28797211/disulfide-bonds-a-key-modification-in-bacterial-extracytoplasmic-proteins
#18
S F Lee, L Davey
Disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. Almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. Accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. Recent advances clearly show that oral bacteria also have the ability to from disulfide bonds, and this ability has an effect on a range of dental plaque-related phenotypes...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28691662/role-of-the-inner-membrane-histidine-kinase-rcsc-and-outer-membrane-lipoprotein-rcsf-in-the-activation-of-the-rcs-phosphorelay-signal-transduction-system-in-escherichia-coli
#19
Takatsugu Sato, Akira Takano, Nanako Hori, Tomoko Izawa, Takayoshi Eda, Kota Sato, Mitsuru Umekawa, Hiroyoshi Miyagawa, Kenji Matsumoto, Ayako Muramatsu-Fujishiro, Kouji Matsumoto, Satoshi Matsuoka, Hiroshi Hara
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. The outer-membrane lipoprotein RcsF is an essential component of the Rcs system. Mislocalization of RcsF to the periplasm or the cytoplasmic membrane leads to high activation of the Rcs system, suggesting that RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. This is consistent with the result reported by Cho et al...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28678862/pseudomonas-aeruginosa-cells-attached-to-a-surface-display-a-typical-proteome-early-as-20-minutes-of-incubation
#20
Marc Crouzet, Stéphane Claverol, Anne-Marie Lomenech, Caroline Le Sénéchal, Patricia Costaglioli, Christophe Barthe, Bertrand Garbay, Marc Bonneu, Sébastien Vilain
Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy...
2017: PloS One
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