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outer membrane protein and biofilm formation

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https://www.readbyqxmd.com/read/28800709/the-major-outer-membrane-protein-mopb-is-required-for-twitching-movement-and-affects-biofilm-formation-and-virulence-in-two-xylella-fastidiosa-strains
#1
Hongyu Chen, Prem Prasad Kandel, Luisa Fernanda Cruz, Paul Anthony Cobine, Leonardo De La Fuente
MopB is a major outer membrane protein in Xylella fastidiosa, a bacterial plant pathogen that causes losses on many economically important crops. Based on in silico analysis, the uncharacterized MopB protein of X. fastidiosa contains a β-barrel structure with an OmpA-like domain and a predicted calcium-binding motif. Here, MopB function was studied by mutational analysis taking advantage of the natural competence of X. fastidiosa. Mutants of mopB were constructed in two different X. fastidiosa strains, the type strain 'Temecula' and the more virulent 'WM1-1'...
August 11, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28797211/disulfide-bonds-a-key-modification-in-bacterial-extracytoplasmic-proteins
#2
S F Lee, L Davey
Disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. Almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. Accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. Recent advances clearly show that oral bacteria also have the ability to from disulfide bonds, and this ability has an effect on a range of dental plaque-related phenotypes...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28691662/role-of-the-inner-membrane-histidine-kinase-rcsc-and-outer-membrane-lipoprotein-rcsf-in-the-activation-of-the-rcs-phosphorelay-signal-transduction-system-in-escherichia-coli
#3
Takatsugu Sato, Akira Takano, Nanako Hori, Tomoko Izawa, Takayoshi Eda, Kota Sato, Mitsuru Umekawa, Hiroyoshi Miyagawa, Kenji Matsumoto, Ayako Muramatsu-Fujishiro, Kouji Matsumoto, Satoshi Matsuoka, Hiroshi Hara
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. The outer-membrane lipoprotein RcsF is an essential component of the Rcs system. Mislocalization of RcsF to the periplasm or the cytoplasmic membrane leads to high activation of the Rcs system, suggesting that RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. This is consistent with the result reported by Cho et al...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28678862/pseudomonas-aeruginosa-cells-attached-to-a-surface-display-a-typical-proteome-early-as-20-minutes-of-incubation
#4
Marc Crouzet, Stéphane Claverol, Anne-Marie Lomenech, Caroline Le Sénéchal, Patricia Costaglioli, Christophe Barthe, Bertrand Garbay, Marc Bonneu, Sébastien Vilain
Biofilms are present in all environments and often result in negative effects due to properties of the biofilm lifestyle and especially antibiotics resistance. Biofilms are associated with chronic infections. Controlling bacterial attachment, the first step of biofilm formation, is crucial for fighting against biofilm and subsequently preventing the persistence of infection. Thus deciphering the underlying molecular mechanisms involved in attachment could allow discovering molecular targets from it would be possible to develop inhibitors against bacterial colonization and potentiate antibiotherapy...
2017: PloS One
https://www.readbyqxmd.com/read/28670572/biological-functions-of-the-secretome-of-neisseria-meningitidis
#5
REVIEW
Jan Tommassen, Jesús Arenas
Neisseria meningitidis is a Gram-negative bacterial pathogen that normally resides as a commensal in the human nasopharynx but occasionally causes disease with high mortality and morbidity. To interact with its environment, it transports many proteins across the outer membrane to the bacterial cell surface and into the extracellular medium for which it deploys the common and well-characterized autotransporter, two-partner and type I secretion mechanisms, as well as a recently discovered pathway for the surface exposure of lipoproteins...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28609638/hyperbiofilm-phenotype-of-pseudomonas-aeruginosa-defective-for-the-plcb-and-plcn-secreted-phospholipases
#6
Shawn Lewenza, Laetitia Charron-Mazenod, Shirin Afroj, Erik van Tilburg Bernardes
Biofilms are dense communities of bacteria enmeshed in a protective extracellular matrix composed mainly of exopolysaccharides, extracellular DNA, proteins, and outer membrane vesicles (OMVs). Given the role of biofilms in antibiotic-tolerant and chronic infections, novel strategies are needed to block, disperse, or degrade biofilms. Enzymes that degrade the biofilm matrix are a promising new therapy. We screened mutants in many of the enzymes secreted by the type II secretion system (T2SS) and determined that the T2SS, and specifically phospholipases, play a role in biofilm formation...
June 13, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28588134/the-rcs-regulated-colanic-acid-capsule-maintains-membrane-potential-in-salmonella-enterica-serovar-typhimurium
#7
Jasmine M Pando, Joyce E Karlinsey, Jimmie C Lara, Stephen J Libby, Ferric C Fang
The Rcs phosphorelay and Psp (phage shock protein) systems are envelope stress responses that are highly conserved in gammaproteobacteria. The Rcs regulon was found to be strongly induced during metal deprivation of Salmonella enterica serovar Typhimurium lacking the Psp response. Nineteen genes activated by the RcsA-RcsB response regulator make up an operon responsible for the production of colanic acid capsular polysaccharide, which promotes biofilm development. Despite more than half a century of research, the physiological function of colanic acid has remained elusive...
June 6, 2017: MBio
https://www.readbyqxmd.com/read/28400767/the-periplasmic-chaperone-network-of-campylobacter-jejuni-evidence-that-salc-cj1289-and-ppid-cj0694-are-involved-in-maintaining-outer-membrane-integrity
#8
Aidan J Taylor, Shadi A I Zakai, David J Kelly
The outer membrane (OM) of Gram-negative pathogenic bacteria is a key structure in host-pathogen interactions that contains a plethora of proteins, performing a range of functions including adhesion, nutrient uptake, export of effectors and interaction with innate and adaptive components of the immune system. In addition, the OM can exclude drugs and thus contribute to antimicrobial resistance. The OM of the food-borne pathogen Campylobacter jejuni contains porins, adhesins and other virulence factors that must be specifically localized to this membrane, but the protein sorting mechanisms involved are only partially understood...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28357362/bacterial-outer-membrane-vesicle-biogenesis-a-new-mechanism-and-its-implications
#9
COMMENT
Sandro Roier, Franz G Zingl, Fatih Cakar, Stefan Schild
Outer membrane vesicle (OMV) release by Gram-negative bacteria has been observed and studied for decades. First considered as a by-product of cell lysis, it soon became evident that OMVs are actively secreted from the outer membrane (OM) of Gram-negative bacteria. Accordingly, these small particles (~ 10-300 nm in diameter) consist mainly of OM components like phospholipids (PLs), OM proteins, and lipopolysaccharides or lipooligosaccharides. However, OMVs may also comprise periplasmic, inner membrane, or cytoplasmic components...
May 10, 2016: Microbial Cell
https://www.readbyqxmd.com/read/28199190/filamentous-hemagglutinin-adhesin-fhab-limits-a-baumannii-biofilm-formation
#10
Shakiba Darwish Alipour Astaneh, Iraj Rasooli, Seyed Latif Mousavi Gargari
Increased resistance and survival, as well as immune evasion, play a significant role in the pathogenicity of Acinetobacter baumannii. Here, we report on the adhesion of the bacterium to epithelial cells and formation of biofilm on abiotic surfaces. We identificed autotransporter (AT) genes that encode homologues (Fha-like) of the two-partner secretion system (TPS) in A.baumannii (ATCC19606) which we designate as FhaB (exoprotein) and FhaC (transporter) and demonstrate that these novel genes, are under the control of distinct regulatable promoters within either the same (FhaBC) or two distinct (FhaB and FhaC) cells...
March 1, 2017: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/28173753/conjugative-type-ivb-pilus-recognizes-lipopolysaccharide-of-recipient-cells-to-initiate-papi-1-pathogenicity-island-transfer-in-pseudomonas-aeruginosa
#11
Toan Phuoc Hong, Michelle Q Carter, Paolo Struffi, Stefano Casonato, Youai Hao, Joseph S Lam, Stephen Lory, Olivier Jousson
BACKGROUND: Pseudomonas aeruginosa pathogenicity island 1 (PAPI-1) is one of the largest genomic islands of this important opportunistic human pathogen. Previous studies have shown that PAPI-1 encodes several putative virulence factors, including a major regulator of biofilm formation and antibiotic-resistance traits. PAPI-1 is horizontally transferable into recipient strains lacking this island via conjugation mediated by the specialized type IV pilus. The PAPI-1 encodes a cluster of ten genes associated with the synthesis and assembly of the type IV pilus...
February 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28173636/identification-and-characterization-of-a-novel-fusobacterium-nucleatum-adhesin-involved-in-physical-interaction-and-biofilm-formation-with-streptococcus-gordonii
#12
Bruno P Lima, Wenyuan Shi, Renate Lux
To successfully colonize the oral cavity, bacteria must directly or indirectly adhere to available oral surfaces. Fusobacterium nucleatum plays an important role in oral biofilm community development due to its broad adherence abilities, serving as a bridge between members of the oral biofilm that cannot directly bind to each other. In our efforts to characterize the molecular mechanisms utilized by F. nucleatum to physically bind to key members of the oral community, we investigated the involvement of F. nucleatum outer membrane proteins in its ability to bind to the pioneer biofilm colonizer, Streptococcus gordonii...
June 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28072820/p-cymene-promotes-its-catabolism-through-the-p-cymene-and-the-p-cumate-pathways-activates-a-stress-response-and-reduces-the-biofilm-formation-in-burkholderia-xenovorans-lb400
#13
Loreine Agulló, María José Romero-Silva, Mirian Domenech, Michael Seeger
p-Cymene is an aromatic terpene that is present in diverse plant species. The aims of this study were to study the p-cymene metabolism in the model aromatic-degrading bacterium Burkholderia xenovorans LB400, and its response to p-cymene. The catabolic p-cymene (cym) and p-cumate (cmt) genes are clustered on the LB400 major chromosome. B. xenovorans LB400 was able to grow on p-cymene as well as on p-cumate as a sole carbon and energy sources. LB400 growth attained higher cell concentration at stationary phase on p-cumate than on p-cymene...
2017: PloS One
https://www.readbyqxmd.com/read/28031283/diversification-of-the-alpb-outer-membrane-protein-of-helicobacter-pylori-affects-biofilm-formation-and-cellular-adhesion
#14
Hideo Yonezawa, Takako Osaki, Toshiyuki Fukutomi, Tomoko Hanawa, Satoshi Kurata, Cynthia Zaman, Fuhito Hojo, Shigeru Kamiya
Helicobacter pylori is one of the most common causes of bacterial infection in humans, and it forms biofilms on human gastric mucosal epithelium as well as on in vitro abiotic surfaces. Bacterial biofilm is critical not only for environmental survival but also for successful infection. We previously demonstrated that strain TK1402, which was isolated from a Japanese patient with duodenal and gastric ulcers, has high biofilm-forming ability in vitro relative to other strains. In addition, we showed that outer membrane vesicles (OMV) play an important role in biofilm formation...
March 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/27988234/deletion-of-the-vacj-gene-affects-the-biology-and-virulence-in-haemophilus-parasuis-serovar-5
#15
Liangyou Zhao, Xueli Gao, Chaonan Liu, Xiaoping Lv, Nan Jiang, Shimin Zheng
Haemophilus parasuis is an important pathogen causing severe infections in pigs. However, the specific bacterial factors that participate in pathogenic process are poorly understood. VacJ protein is a recently discovered outer membrane lipoprotein that relates to virulence in several pathogens. To characterize the function of the vacJ gene in H. parasuis virulent strain HS49, a vacJ gene-deletion mutant ΔvacJ and its complemented strain were constructed. Our findings supported that VacJ is essential for maintenance of cellular integrity and stress tolerance of H...
March 1, 2017: Gene
https://www.readbyqxmd.com/read/27892689/biofilm-inhibitors-targeting-the-outer-membrane-protein-a-of-pasteurella-multocida-in-swine
#16
Anchanee Kubera, Arinthip Thamchaipenet, Menachem Shoham
Pasteurella multocida (Pm) is the causative agent of atrophic rhinitis in swine. This study aimed to discover biofilm inhibitors against swine Pm to counteract antibiotic resistance and decrease virulence. The virulence factor outer membrane protein A (OmpA) was targeted. A library of drugs approved by the Food and Drug Administration (FDA) was used to perform virtual screening against PmOmpA. The top-scoring compounds had no effect on the growth of Pm serotype A or D. Mycophenolate mofetil showed the highest efficacy in inhibiting biofilm formation by Pm serotype A, with an IC50 of 7...
January 2017: Biofouling
https://www.readbyqxmd.com/read/27870886/comparative-genomics-revealed-multiple-helicobacter-pylori-genes-associated-with-biofilm-formation-in-vitro
#17
COMPARATIVE STUDY
Eric Hong Jian Wong, Chow Goon Ng, Eng Guan Chua, Alfred Chin Yen Tay, Fanny Peters, Barry J Marshall, Bow Ho, Khean Lee Goh, Jamuna Vadivelu, Mun Fai Loke
BACKGROUND: Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. MATERIALS AND METHODS: Biofilm yield of 32 Helicobacter pylori strains (standard strain and 31 clinical strains) were determined by crystal-violet assay and grouped into poor, moderate and good biofilm forming groups. Whole genome sequencing of these 32 clinical strains was performed on the Illumina MiSeq platform...
2016: PloS One
https://www.readbyqxmd.com/read/27771071/differential-expression-of-putative-adhesin-genes-of-actinobacillus-suis-grown-in-in-vivo-like-conditions
#18
Adina R Bujold, Josée Labrie, Mario Jacques, Janet I MacInnes
Actinobacillus suis is an opportunistic pathogen that resides in the tonsils of the soft palate of swine. Unknown stimuli can cause this organism to invade the host, resulting in septicaemia and sequelae including death. To better understand its pathogenesis, the expression of several adhesin genes was evaluated by semi-quantitative real-time PCR in A. suis grown in conditions that mimic the host environment, including different nutrient and oxygen levels, exponential and stationary phases of growth, and in the presence of the stress hormone epinephrine...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27672084/impact-of-moderate-temperature-changes-on-neisseria-meningitidis-adhesion-phenotypes-and-proteome
#19
Martin Lappann, Andreas Otto, Madita Brauer, Dörte Becher, Ulrich Vogel, Kay Johswich
Neisseria meningitidis, the meningococcus, bears the potential to cause life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared the levels of meningococcal biofilm formation, autoaggregation, and cellular adherence at 32°C and 37°C and found a clear increase in all these phenotypes at 32°C suggestive of a stronger in vivo colonization capability at this temperature...
December 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27595778/role-of-ompa2-surface-regions-of-porphyromonas-gingivalis-in-host-pathogen-interactions-with-oral-epithelial-cells
#20
Kathryn L Naylor, Magdalena Widziolek, Stuart Hunt, Mary Conolly, Matthew Hicks, Prachi Stafford, Jan Potempa, Craig Murdoch, C W Ian Douglas, Graham P Stafford
Outer membrane protein A (OmpA) is a key outer membrane protein found in Gram-negative bacteria that contributes to several crucial processes in bacterial virulence. In Porphyromonas gingivalis, OmpA is predicted as a heterotrimer of OmpA1 and OmpA2 subunits encoded by adjacent genes. Here we describe the role of OmpA and its individual subunits in the interaction of P. gingivalis with oral cells. Using knockout mutagenesis, we show that OmpA2 plays a significant role in biofilm formation and interaction with human epithelial cells...
February 2017: MicrobiologyOpen
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