keyword
MENU ▼
Read by QxMD icon Read
search

antibiotic resistance and biofilm formation

keyword
https://www.readbyqxmd.com/read/28108098/comparative-efficacies-of-daptomycin-vancomycin-and-linezolid-in-experimental-enterococcal-peritonitis
#1
Toshiki Kajihara, Shigeki Nakamura, Naoki Iwanaga, Kazuhiro Oshima, Katsuji Hirano, Taiga Miyazaki, Koichi Izumikawa, Katsunori Yanagihara, Yoshitsugu Miyazaki, Noboru Hattori, Nobuoki Kohno, Shigeru Kohno, Hiroshi Mukae
Enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. Although the in vitro susceptibility of daptomycin (DAP) against Enterococci is well established, the Food and Drug Administration has only approved its use for complicated skin and skin structure infections induced by Enterococcus faecalis. In this study we evaluated the potential therapeutic application of DAP in a murine model of enterococcal experimental peritonitis...
January 17, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28106797/the-complex-relationship-between-virulence-and-antibiotic-resistance
#2
REVIEW
Meredith Schroeder, Benjamin D Brooks, Amanda E Brooks
Antibiotic resistance, prompted by the overuse of antimicrobial agents, may arise from a variety of mechanisms, particularly horizontal gene transfer of virulence and antibiotic resistance genes, which is often facilitated by biofilm formation. The importance of phenotypic changes seen in a biofilm, which lead to genotypic alterations, cannot be overstated. Irrespective of if the biofilm is single microbe or polymicrobial, bacteria, protected within a biofilm from the external environment, communicate through signal transduction pathways (e...
January 18, 2017: Genes
https://www.readbyqxmd.com/read/28087484/inhibition-of-bacterial-adhesion-and-biofilm-formation-by-dual-functional-textured-and-nitric-oxide-releasing-surfaces
#3
Li-Chong Xu, Yaqi Wo, Mark E Meyerhoff, Christopher A Siedlecki
: In separate prior studies, physical topographic surface modification or nitric oxide (NO) release has been demonstrated to each be an effective approach to inhibit and control bacterial adhesion and biofilm formation on polymeric surfaces. Such approaches can prevent biomaterial-associated infection without causing the antibiotic resistance of the strain. In this work, both techniques were successfully integrated and applied to a polyurethane (PU) biomaterial surface that bears ordered pillar topographies (400/400 nm and 500/500 nm patterns) at the top surface and a S-nitroso-N-acetylpenicillamine (SNAP, NO donor) doped sub-layer in the middle, via a soft lithography two-stage replication process...
January 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087472/extract-of-clinopodium-bolivianum-protects-against-e-coli-invasion-of-uroepithelial-cells
#4
Soumitra Mohanty, Witchuda Kamolvit, Silvia Zambrana, Corine Sandström, Eduardo Gonzales, Claes-Göran Östenson, Annelie Braunerf
ETHNOPHARMACOLOGICAL RELEVANCE: Clinopodium bolivianum is a South American plant with anti-inflammatory and anti-infective activities. The increasing antibiotic resistance urges for alternative therapy. Based on its use in traditional medicine, we investigated the effect of C. bolivianum on the ability to defend bladder epithelial cells from E. coli infection. MATERIALS AND METHODS: The extract was analysed by LC-MS. Bladder epithelial cell lines T24 and 5637 and uropathogenic E...
January 10, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28081347/virulence-factors-biofilm-forming-ability-and-antimicrobial-resistance-of-urinary-escherichia-coli-strains-isolated-from-hospitalized-patients
#5
Barbara Kot, Jolanta Wicha, Agata Grużewska, Małgorzata Piechota, Katarzyna Wolska, Maria Obrębska
BACKGROUND/AIM: Escherichia coli is the most frequent cause of urinary tract infections. We investigated the possible associations between the origin of strains, antimicrobial resistance, the presence of urovirulence factors, and biofilm-forming ability. MATERIALS AND METHODS: Antibiotic susceptibility of E. coli strains was tested by disk diffusion method. Hemagglutination assays were performed for phenotypic characterization of the cell surface. Multiplex PCR was used for detection of virulence genes and for determination of phylogenetic relationships...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28077265/a-smart-aminoglycoside-hydrogel-with-tunable-gel-degradation-on-demand-drug-release-and-high-antibacterial-activity
#6
Jingjing Hu, Yanchun Quan, Yuping Lai, Zhao Zheng, Zhiqi Hu, Xinyu Wang, Tianjiao Dai, Qiang Zhang, Yiyun Cheng
Aminoglycosides are a family of critically important antibiotics for the treatment of serious infections including multidrug-resistant pathogens. However, clinical use of aminoglycoside antibiotics is compromised by bacterial biofilm formation at subinhibitory concentrations or adverse side effects such as ototoxicity and nephrotoxicity at high antibiotic doses. Preparation of aminoglycoside formulation that allows on-demand drug delivery is a solution to this sticky issue. Here, we designed a new type of aminoglycoside hydrogels by cross-linking oxidized polysaccharides such as dextran, carboxymethyl cellulose, alginate, and chondroitin using aminoglycosides as cross-linkers...
January 7, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28070537/molecular-analysis-and-expression-of-bap-gene-in-biofilm-forming-multi-drug-resistant-acinetobacter-baumannii
#7
Azizi Omid, Shahcheraghi Fereshteh, Salimizand Himen, Modarresi Farzan, Shakibaie Mohammad Reza, Mansouri Shahla, Ramazanzadeh Rashid, Badmasti Farzad, Nikbin Vajihe
BACKGROUND: Acinetobacter baumannii is commonly resistant to nearly all antibiotics due to presence of antibiotic resistance genes and biofilm formation. In this study we determined the presence of certain antibiotic-resistance genes associated with biofilm production and the influence of low iron concentration on expression of the biofilm-associated protein gene (bap) in development of biofilm among multi-drug-resistant A. baumannii (MDRAB). METHODS: Sixty-five MDRAB isolates from clinical samples were collected...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28069377/development-of-an-aptamer-ampicillin-conjugate-for-treating-biofilms
#8
Cheng Lijuan, Yan Xing, Wu Minxi, Li Wenkai, Deng Le
Biofilm formation involves the development of extracellular matrix and initially depends on adherence and tropism by flagellar movement. With the widespread development of antibiotic resistance and tolerance of biofilms, there is a growing need for novel anti-infective strategies. No currently approved medications specifically target biofilms. Aptamers are single-stranded nucleic acid molecules that may bind to their targets with high affinity and affect the target functions. We developed a bifunctional conjugate by linking an aptamer targeting bacterial flagella with ampicillin...
January 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28062294/assessment-of-biofilm-formation-in-pseudomonas-aeruginosa-by-antisense-maze-pna
#9
Hassan Valadbeigi, Nourkhoda Sadeghifard, Majid Baseri Salehi
The hallmark patogenicity in Pseudomonas aeruginosa (P. aeruginosa) is biofilm formation that is not easy to eradicate, because it has variety mechanisms for antibiotic resistance. In addition, toxin-antitoxin (TA) system may play role in biofilm formation. The current study aimed to evaluate the role of TA loci in biofilm formation. Therefore, 18 P. aeruginosa clinical isolates were collected and evaluated for specific biofilm and TA genes. The analysis by RT-qPCR demonstrated that expression of mazE antitoxin in biofilm formation was increase...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28062292/use-of-carvacrol-thymol-and-eugenol-for-biofilm-eradication-and-resistance-modifying-susceptibility-of-salmonella-enterica-serovar-typhimurium-strains-to-nalidixic-acid
#10
Hanene Miladi, Tarek Zmantar, Bochra Kouidhi, Yassine Chaabouni, Kacem Mahdouani, Amina Bakhrouf, Kamel Chaieb
The Aims of the study was to evaluate the antibacterial susceptibility and the biofilm eradication of three natural compounds carvacrol (CAR), thymol (TH) and eugenol (EUG), alone or in combination with nalidixic acid (NA) against twelve Salmonella Typhimurium strains. The minimum inhibitory concentration (MIC) and the minimum biofilm eradication concentration (BEC50) of the tested compounds (CAR, TH and EUG) and their combinations with NA were evaluated. In order to assess whether these bacteria had active efflux pumps, ethidium bromide (EtBr) accumulation assays was achieved using spectrophotometric accumulation assays...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28062284/population-dynamics-antibiotics-resistance-and-biofilm-formation-of-aeromonas-and-vibrio-species-isolated-from-aquatic-sources-in-northern-malaysia
#11
Olumide A Odeyemi, Asmat Ahmad
This study aimed to compare population dynamics, antibiotic resistance and biofilm formation of Aeromonas and Vibrio species from seawater and sediment collected from Northern Malaysia. Isolates with different colony morphology were characterized using both biochemical and molecular methods before testing for antibiotic resistance and biofilm formation. Results obtained from this study showed that in Kedah, the population of Aeromonas isolated from sediment was highest in Pantai Merdeka (8.22 log CFU/ml), Pulau Bunting recorded the highest population of Aeromonas from sediment (8...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28056745/exploiting-quorum-sensing-inhibition-for-the-control-of-pseudomonas-aeruginosa-and-acinetobacter-baumannii-biofilms
#12
Israel Castillo-Juarez, Luis Esaú López-Jácome, Gloria Soberón-Chávez, María Tomás, Jintae Lee, Paulina Castañeda-Tamez, Iván Ángelo Hernández-Bárragan, Martha Yumiko Cruz-Muñiz, Toshinari Maeda, Thomas K Wood, Rodolfo García-Contreras
Pseudomonas aeruginosa and Acinetobacter baumannii are two of the main bacteria responsible for nosocomial infections; both organisms are resistant to several classes of antibiotics making their infections very difficult to treat. Moreover, they possess a remarkable ability to form biofilms, which further enhances their antimicrobial resistance. Both organisms coordinate their formation of biofilms and their expression of virulence factors through quorum sensing, a system that regulates gene expression at high cell densities and that plays a key role in the establishment of bacterial infections...
January 5, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28056743/antimicrobial-peptides-for-the-control-of-biofilm-formation
#13
Mercedes González Moreno, Lisa Lombardi, Mariagrazia Di Luca
Antimicrobial peptides (AMPs) are an abundant and varied group of molecules recognized as the most ancient components of the innate immune system. They are found in a wide group of organisms including bacteria, plants and animals as a defense mechanism against different kinds of infectious pathogens. Over the past two decades, a fast-growing number of AMPs have been identified/designed and their wide-spectrum antimicrobial activity has been deeply investigated. In recent years, there has been an increasing interest in the use of AMPs as alternative anti-biofilm molecules for the control of biofilm-related infections...
January 5, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28049020/eradication-ofpseudomonas-aeruginosacells-by-cathodic-electrochemical-currents-delivered-with-graphite-electrodes
#14
Tagbo H R Niepa, Hao Wang, Jeremy L Gilbert, Dacheng Ren
Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we reported a new approach to eradicating persister cells of Pseudomonas aeruginosa using low-level direct electrochemical current (DC) and synergy with the antibiotic tobramycin. To further understand the underlying mechanism and develop this technology toward possible medical applications, we investigated the electricidal activities of non-metallic biomaterial on persister and biofilm cells of P...
December 31, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28045495/bacterial-networks-on-hydrophobic-micropillars
#15
Zeinab Jahed, Hamed Shahsavan, Mohit S Verma, Jacob L Rogowski, Brandon B Seo, Boxin Zhao, Ting Y Tsui, Frank X Gu, Mohammad R K Mofrad
Bacteria have evolved as intelligent microorganisms that can colonize and form highly structured and cooperative multicellular communities with sophisticated singular and collective behaviors. The initial stages of colony formation and intercellular communication are particularly important to understand and depend highly on the spatial organization of cells. Controlling the distribution and growth of bacterial cells at the nanoscale is, therefore, of great interest in understanding the mechanisms of cell-cell communication at the initial stages of colony formation...
January 9, 2017: ACS Nano
https://www.readbyqxmd.com/read/28045163/crispr-cas9-technology-applications-in-genome-engineering-development-of-sequence-specific-antimicrobials-and-future-prospects
#16
REVIEW
César de la Fuente-Núñez, Timothy K Lu
The development of CRISPR-Cas9 technology has revolutionized our ability to edit DNA and to modulate expression levels of genes of interest, thus providing powerful tools to accelerate the precise engineering of a wide range of organisms. In addition, the CRISPR-Cas system can be harnessed to design "precision" antimicrobials that target bacterial pathogens in a DNA sequence-specific manner. This capability will enable killing of drug-resistant microbes by selectively targeting genes involved in antibiotic resistance, biofilm formation and virulence...
January 3, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28039075/comparative-genome-analysis-of-multiple-vancomycin-resistant-enterococcus-faecium-isolated-from-two-fatal-cases
#17
Shu Yong Lim, Kien-Pong Yap, Cindy Shuan Ju Teh, Kartini Abdul Jabar, Kwai Lin Thong
Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VREfm) strains isolated from two fatal cases in a tertiary hospital in Malaysia. Two sequence types, ST80 and ST203, were identified which belong to the clinically important clonal complex (CC) 17...
December 28, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28034758/ymdb-mediated-down-regulation-of-suca-inhibits-biofilm-formation-and-induces-apramycin-susceptibility-in-escherichia-coli
#18
Moonjeong Kim, Minji Kim, Kwang-Sun Kim
Antibiotic resistance associated with biofilm formation is a major concern when treating bacterial infections with drugs. The genes and pathways involved in biofilm formation have been extensively studied and are also involved in antibiotic resistance. Recent studies show that overexpression of Escherichia coli (E. coli) YmdB protein alters gene expression profiles and inhibits biofilm formation. Therefore, it is expected that YmdB and its regulated genes play a key role in development of biofilm and antibiotic resistance phenotypes...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28034294/probiotic-properties-of-lactobacillus-fermentum-strains-isolated-from-human-oral-samples-and-description-of-their-antibacterial-activity
#19
Virginia Fuochia, Giovanni Li Volti, Pio Maria Furneri
BACKGROUND: Gram positive bacteria produce peptides, defined bacteriocins which exhibit good antibacterial activity. OBJECTIVE: We evaluated the ability of L. fermentum to produce bacteriocins having therefore, good probiotic features and finally, be safe towards microglial cells. METHOD: Eight wild strains, identified using molecular techniques, were investigated for the evaluation of resistance to bile salts, low pH, H2O2 production, biofilm formation, antibacterial activity and safety on microglia cells (BV2)...
December 29, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28033847/impact-of-polymicrobial-biofilms-in-catheter-associated-urinary-tract-infections
#20
Andreia S Azevedo, Carina Almeida, Luís F Melo, Nuno F Azevedo
Recent reports have demonstrated that most biofilms involved in catheter-associated urinary tract infections are polymicrobial communities, with pathogenic microorganisms (e.g. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) and uncommon microorganisms (e.g. Delftia tsuruhatensis, Achromobacter xylosoxidans) frequently co-inhabiting the same urinary catheter. However, little is known about the interactions that occur between different microorganisms and how they impact biofilm formation and infection outcome...
December 30, 2016: Critical Reviews in Microbiology
keyword
keyword
116560
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"