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antibiotic resistance and biofilm formation

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https://www.readbyqxmd.com/read/29473844/antimicrobial-and-antibiofilm-activity-and-machine-learning-classification-analysis-of-essential-oils-from-different-mediterranean-plants-against-pseudomonas-aeruginosa
#1
Marco Artini, Alexandros Patsilinakos, Rosanna Papa, Mijat Božović, Manuela Sabatino, Stefania Garzoli, Gianluca Vrenna, Marco Tilotta, Federico Pepi, Rino Ragno, Laura Selan
Pseudomonas aeruginosa is a ubiquitous organism and opportunistic pathogen that can cause persistent infections due to its peculiar antibiotic resistance mechanisms and to its ability to adhere and form biofilm. The interest in the development of new approaches for the prevention and treatment of biofilm formation has recently increased. The aim of this study was to seek new non-biocidal agents able to inhibit biofilm formation, in order to counteract virulence rather than bacterial growth and avoid the selection of escape mutants...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29463543/mesoscopic-energy-minimization-drives-pseudomonas-aeruginosa-biofilm-morphologies-and-consequent-stratification-of-antibiotic-activity-based-on-cell-metabolism
#2
M V Sheraton, K H Yam, C H Tan, H S Oh, E Mancini, L Yang, S A Rice, P M A Sloot
Segregation of bacteria based on their metabolic activity in biofilms plays an important role in the development of antibiotic drug resistance. Mushroom-shaped biofilm structures, which are reported for many bacteria, exhibit topographically varying levels of multiple drug resistance from the cap of the mushroom to its stalk. Understanding the dynamics behind the formation of such structures can aid in design of drug delivery systems, antibiotics, or physical systems for removal of biofilms. We explore the development of metabolically heterogenous Pseudomonas aeruginosa biofilms using numerical models and laboratory knock-out experiments on wild-type and chemotaxis deficient mutants...
February 20, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29463534/alginate-oligosaccharide-induced-modification-of-the-lasi-lasr-and-rhli-rhlr-quorum-sensing-systems-in-pseudomonas-aeruginosa
#3
Alison A Jack, Saira Khan, Lydia C Powell, Manon F Pritchard, Konrad Beck, Hina Sadh, Lucy Sutton, Alessandra Cavaliere, Hannah Florance, Philip D Rye, David W Thomas, Katja E Hill
Pseudomonas aeruginosa plays a major role in many chronic infections. Its ability to readily form biofilms contributes to its success as an opportunistic pathogen and its resistance/tolerance to antimicrobial/antibiotic therapy. A low molecular weight alginate oligomer (OligoG CF-5/20), derived from marine algae, has previously been shown to impair motility in P. aeruginosa biofilms and disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG CF-5/20 may induce alterations in QS signalling in P...
February 20, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29462909/antimicrobial-peptides-for-topical-treatment-of-osteomyelitis-and-implant-related-infections-study-in-the-spongy-bone
#4
Pavel Melicherčík, Ondřej Nešuta, Václav Čeřovský
We examined the benefits of short linear α-helical antimicrobial peptides (AMPs) invented in our laboratory for treating bone infection and preventing microbial biofilm formation on model implants due to causative microorganisms of osteomyelitis. For this purpose, we introduced a model of induced osteomyelitis that utilizes human femur heads obtained from the hospital after their replacement with artificial prostheses. We found that the focus of the infection set up in the spongy part of this bone treated with AMP-loaded calcium phosphate cement was eradicated much more effectively than was the focus treated with antibiotics such as vancomycin or gentamicin loaded into the same cement...
February 16, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29462728/treatment-of-lumbar-discitis-using-silicon-nitride-spinal-spacers-a-case-series-and-literature-review
#5
William M Rambo
INTRODUCTION: Septic infection of a lumbar intervertebral disc is a serious disorder which is often difficult to diagnose and appropriately treat because of the rarity of the disease, the varied presentation of symptoms, and the frequency of low-back pain within the overall population. Its etiology can be pyogenic, granulomatous, fungal, or parasitic; its incidence is rising due to increased patient susceptibility and improved diagnostic tools. Conservative treatments involve antibiotics, physical therapy, and/or immobilization...
February 10, 2018: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29460278/structural-and-metabolic-responses-of-staphylococcus-aureus-biofilms-to-hyperosmotic-and-antibiotic-stress
#6
Mia Mae Kiamco, Abdelrhman Mohamed, Patrick Reardon, Carrie Marean-Reardon, Wrya Moh Aframehr, Douglas R Call, Haluk Beyenal, Ryan Renslow
Biofilms alter their metabolism in response to environmental stress. This study explores the effect of a hyperosmotic agent-antibiotic treatment on the metabolism of Staphylococcus aureus biofilms through the use of nuclear magnetic resonance (NMR) techniques. To determine the metabolic activity of S. aureus, we quantified the concentrations of metabolites in spent medium using high-resolution NMR spectroscopy. Biofilm porosity, thickness, biovolume, and relative diffusion coefficient depth profiles were obtained using NMR microimaging...
February 20, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29433269/genetic-relatedness-antimicrobial-resistance-and-biofilm-formation-of-salmonella-isolated-from-naturally-contaminated-poultry-and-their-processing-environment-in-northern-malaysia
#7
Li-Oon Chuah, Ahamed-Kamal Shamila Syuhada, Ismail Mohamad Suhaimi, Tajudin Farah Hanim, Gulam Rusul
We investigated the genetic relatedness, antibiotic resistance and biofilm-producing ability of 114 strains of Salmonella, belonged to three serotypes (Corvallis, Brancaster and Albany), isolated from naturally contaminated poultry and their environment in wet markets and smale-scale processing plant from northern Malaysia. Pulsed-field gel electrophoresis revealed that Salmonella strains isolated from various wet markets were clonally related, suggesting the widespread dissemination of these three serotypes in northern Malaysia...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29428742/short-communication-roles-of-outer-membrane-protein-w-ompw-on-survival-and-biofilm-formation-of-cronobacter-sakazakii-under-neomycin-sulfate-stress
#8
Yingwang Ye, Na Ling, Jina Gao, Maofeng Zhang, Xiyan Zhang, Liaowang Tong, Dexin Ou, Yaping Wang, Jumei Zhang, Qingping Wu
Cronobacter sakazakii is associated with severe infections including sepsis, neonatal meningitis, and necrotizing enterocolitis. Antibiotic resistance in Cronobacter species has been documented in recent years, but the genes involved in resistance in Cronobacter strains are poorly understood. In this study, we determined the role of outer membrane protein W (OmpW) on survival rates, morphologic changes, and biofilm formation between wild type (WT) and an OmpW mutant strain (ΔOmpW) under neomycin sulfate stress...
February 7, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29427473/antimicrobial-and-cytotoxic-activity-of-silver-nanoparticles-synthesized-from-two-haloalkaliphilic-actinobacterial-strains-alone-and-in-combination-with-antibiotics
#9
Magdalena Wypij, Magdalena Świecimska, Joanna Czarnecka, Hanna Dahm, Mahendra Rai, Patrycja Golinska
AIMS: Presently, the effective antimicrobial agents have been limited by emergence of microbial strains with multi-drug resistance and biofilm formation potential. In the present study, we report remarkable antimicrobial activity of silver nanoparticles (AgNPs) synthesized from Streptomyces calidiresistens IF11 and IF17 strains, including inhibition of biofilm formation and synergistic effect of AgNPs and antibiotics against selected bacteria and yeasts. Cytotoxic effect of AgNPs on mammalian cell lines was also evaluated...
February 10, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29427090/extracellular-red-monascus-pigment-mediated-rapid-one-step-synthesis-of-silver-nanoparticles-and-its-application-in-biomedical-and-environment
#10
Sunil H Koli, Bhavana V Mohite, Rahul K Suryawanshi, Hemant P Borase, Satish V Patil
The development of a safe and eco-friendly method for metal nanoparticle synthesis has an increasing demand, due to emerging environmental and biological harms of hazardous chemicals used in existing nanosynthesis methods. The present investigation reports a rapid one-step, eco-friendly and green approach for the formation of nanosized silver particles (AgNPs) using extracellular non-toxic-colored fungal metabolites (Monascus pigments-MPs). The formation of nanosized silver particles utilizing Monascus pigments was confirmed after exposure of reaction mixture to sunlight, by visually color change and further established by spectrophotometric analysis...
February 10, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29426873/suppression-of-staphylococcus-aureus-biofilm-formation-and-virulence-by-a-benzimidazole-derivative-um-c162
#11
Cin Kong, Chin-Fei Chee, Katharina Richter, Nicky Thomas, Noorsaadah Abd Rahman, Sheila Nathan
Staphylococcus aureus is a major cause of nosocomial infections and secretes a diverse spectrum of virulence determinants as well as forms biofilm. The emergence of antibiotic-resistant S. aureus highlights the need for alternative forms of therapeutics other than conventional antibiotics. One route to meet this need is screening small molecule derivatives for potential anti-infective activity. Using a previously optimized C. elegans - S. aureus small molecule screen, we identified a benzimidazole derivative, UM-C162, which rescued nematodes from a S...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29426802/development-of-mycoplasma-pneumoniae-biofilms-in-vitro-and-the-limited-role-of-motility
#12
Monica Feng, Andrew C Schaff, Sara A Cuadra Aruguete, Hailey E Riggs, Steven L Distelhorst, Mitchell F Balish
Mycoplasma pneumoniae is a bacterial pathogen of humans that is a major causative agent of chronic respiratory disease. M. pneumoniae infections often recur even after successful treatment of symptoms with antibiotics, and resistance to antibiotics is increasing worldwide, with nearly complete resistance in some places. Although biofilms often contribute to chronicity and resistance, M. pneumoniae biofilms remain poorly characterized. Scanning electron microscopy revealed that cells of wild-type (WT) M. pneumoniae strain M129 biofilms, as well as mutants II-3 and II-3R, in vitro became increasingly rounded as the biofilm towers matured over 5 days...
February 7, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29421548/bacteria-responsive-intelligent-wound-dressing-simultaneous-in-situ-detection-and-inhibition-of-bacterial-infection-for-accelerated-wound-healing
#13
Jin Zhou, Danyu Yao, Zhiyong Qian, Sen Hou, Linhao Li, A Tobias A Jenkins, Yubo Fan
The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist...
January 18, 2018: Biomaterials
https://www.readbyqxmd.com/read/29421318/antibacterial-resistance-biofilm-forming-ability-and-virulence-potential-of-pseudomonas-aeruginosa-isolated-from-burn-patients-in-northern-of-iran
#14
Leila Asadpour
OBJECTIVES: Pseudomonas aeruginosa is frequent in infectious diseases, such as burn and wound infections, making it one of the most promising opportunistic pathogens. The aim of this study was to investigate the antibiotic resistance, biofilm production capacity, and the frequency of genes, which are effective in the biofilm formation and virulence of Pseudomonas aeruginosa, from burn infections. METHODS: In this study, resistance of 90 P. aeruginosa strains to twelve antibacterial agents and extended-spectrum beta-lactamases (ESBLs) and Metallo-β-lactamases(MBL) enzymes production were phenotypically assessed according to Clinical & Laboratory Standards Institute guidelines...
February 5, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29413589/chitosan-nanoencapsulation-of-flavonoids-enhances-their-quorum-sensing-and-biofilm-formation-inhibitory-activities-against-an-e-coli-top-10-biosensor
#15
E O Omwenga, A Hensel, A Shitandi, F M Goycoolea
Phytochemicals have been found to be promising alternatives to conventional antibiotic therapies for the control of bacterial infections, as they may entail less selective pressure and hence reduce the development of resistance. This study involved examining the inhibition of biofilm formation and of quorum sensing (QS), and the cytotoxicity on mammalian cells of two flavonoids, quercetin and baicalein, in free form and associated into chitosan-based nanocapsules. This was done by use of a transformed E. coli Top 10 biosensor strain, while the cytotoxicity was evaluated on MDCK-C7 cells...
January 31, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29412515/l-methionine-anti-biofilm-activity-against-pseudomonas-aeruginosa-is-enhanced-by-the-cystic-fibrosis-transmembrane-conductance-regulator-potentiator-ivacaftor
#16
Do-Yeon Cho, Dong-Jin Lim, Calvin Mackey, Christopher G Weeks, Jaime A Peña Garcia, Daniel Skinner, Jessica W Grayson, Harrison S Hill, David K Alexander, Shaoyan Zhang, Bradford A Woodworth
BACKGROUND: Biofilms may contribute to refractory chronic rhinosinusitis (CRS), as they lead to antibiotic resistance and failure of effective clinical treatment. l-Methionine is an amino acid with reported biofilm-inhibiting properties. Ivacaftor is a cystic fibrosis transmembrane conductance regulator (CFTR) potentiator with mild antimicrobial activity via inhibition of bacterial DNA gyrase and topoisomerase IV. The objective of this study was to evaluate whether co-treatment with ivacaftor and l-methionine can reduce the formation of Pseudomonas aeruginosa biofilms...
February 7, 2018: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/29407752/presence-of-the-esp-gene-in-enterococcus-faecium-derived-from-oropharyngeal-microbiota-of-haematology-patients
#17
Milica Jovanović, Tanja Tošić, Snežana Jovanović, Rajica Stošović, Goran Stevanović, Branko Velebit, Marcus John Zervos
OBJECTIVES: Antibiotic use and immunocompromised status in haematology patients have been shown to determine the constituents of commensal microbiota with highly increased resistance, including vancomycin resistant enterococci. We compared the carriage of virulence factor genes and the capacity for biofilm formation in vancomycin resistant enterococci (VRE) originating from the oropharyngeal and stool cultures of haematology patients. DESIGN: PCR tests were used to investigate the presence of genes encoding pathogenicity factors (esp and hyl) in VRE isolates...
January 30, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29405384/in-vitro-antibiofilm-and-antiadhesion-effects-of-magnesium-oxide-nanoparticles-against-antibiotic-resistant-bacteria
#18
Sumreen Hayat, Saima Muzammil, Muhammad Hidayat Rasool, Zonaira Nisar, Syed Zajif Hussain, Anjum Nasim Sabri, Saba Jamil
The aim of the current investigation was to determine antibacterial and antibiofilm potential of magnesium oxide (MgO) nanoparticles against antibiotic resistant clinical strains of bacteria. MgO nanoparticles were synthesized by wet chemical method, and were further characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Antibacterial activity was determined by broth microdilution and agar diffusion method. Bradford method was used to assess cellular protein leakage as a result of loss of membrane integrity...
February 6, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29398539/synthesis-kinetics-and-inhibition-of-escherichia-coli-heptosyltransferase-i-by-monosaccharide-analogues-of-lipid-a
#19
Noreen K Nkosana, Daniel J Czyzyk, Zarek S Siegel, Joy M Cote, Erika A Taylor
Gram-negative bacteria comprise the majority of microbes that cause infections that are resistant to pre-existing antibiotics. The complex cell wall architecture contributes to their ability to form biofilms, which are often implicated in hospital-acquired infections. Biofilms promote antibiotic resistance by enabling the bacteria to survive hostile environments such as UV radiation, pH shifts, and antibiotics. The outer membrane of Gram-negative bacteria contains lipopolysaccharide (LPS), which plays a role in adhesion to surfaces and formation of biofilms...
February 2, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29392390/strategies-to-prevent-the-occurrence-of-resistance-against-antibiotics-by-using-advanced-materials
#20
REVIEW
Arnau Bassegoda, Kristina Ivanova, Eva Ramon, Tzanko Tzanov
Drug resistance occurrence is a global healthcare concern responsible for the increased morbidity and mortality in hospitals, time of hospitalisation and huge financial loss. The failure of the most antibiotics to kill "superbugs" poses the urgent need to develop innovative strategies aimed at not only controlling bacterial infection but also the spread of resistance. The prevention of pathogen host invasion by inhibiting bacterial virulence and biofilm formation, and the utilisation of bactericidal agents with different mode of action than classic antibiotics are the two most promising new alternative strategies to overcome antibiotic resistance...
February 1, 2018: Applied Microbiology and Biotechnology
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