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antibiofilm activities

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https://www.readbyqxmd.com/read/27902776/inhibition-of-candida-albicans-biofilm-formation-by-the-synthetic-lactoferricin-derived-peptide-hlf1-11
#1
Paola Morici, Roberta Fais, Cosmeri Rizzato, Arianna Tavanti, Antonella Lupetti
The aim of this study was to evaluate the in vitro activity of the synthetic peptide hLF1-11 against biofilm produced by clinical isolates of Candida albicans with different fluconazole susceptibility. The antibiofilm activity of the peptide hLF1-11 was assessed in terms of reduction of biofilm cellular density, metabolic activity and sessile cell viability. The extent of morphogenesis in hLF1-11 treated and untreated biofilms was also investigated microscopically. Transcription levels of genes related to cell adhesion, hyphal development and extracellular matrix production were analysed by qRT-PCR in hLF1-11 treated and untreated biofilms...
2016: PloS One
https://www.readbyqxmd.com/read/27864804/activity-of-norspermidine-on-bacterial-biofilms-of-multidrug-resistant-clinical-isolates-associated-with-persistent-extremity-wound-infections
#2
Anthony P Cardile, Ronald L Woodbury, Carlos J Sanchez, Sandra C Becerra, Rebecca A Garcia, Katrin Mende, Joseph C Wenke, Kevin S Akers
Biofilm formation is a major virulence factor for numerous pathogenic bacteria and is cited as a central event in the pathogenesis of chronic human infections, which is in large part due to excessive extracellular matrix secretion and metabolic changes that occur within the biofilm rendering them highly tolerant to antimicrobial treatments. Polyamines, including norspermidine, play central roles in bacterial biofilm development, but have also recently been shown to inhibit biofilm formation in select strains of various pathogenic bacteria...
November 19, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27864779/activity-of-novel-inhibitors-of-staphylococcus-aureus-biofilms
#3
Seung-Gyun Woo, So-Yeon Lee, So-Min Lee, Kyoung-Hee Lim, Eun-Ju Ha, Yong-Bin Eom
Staphylococcus aureus is one of the most important pathogens causing chronic biofilm infections. These are becoming more difficult to treat owing to drug resistance, particularly because S. aureus biofilms limit the efficacy of antimicrobial agents, leading to high morbidity and mortality. In the present study, we screened for inhibitors of S. aureus biofilm formation using a natural product library from the Korea Chemical Bank (KCB). Screening by crystal violet-based biomass staining assay identified hit compounds...
November 18, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27862685/topical-application-of-probiotics-in-skin-adhesion-antimicrobial-and-antibiofilm-in-vitro-assays
#4
Elísia G Lopes, Diana A Moreira, Patricia Gullón, Beatriz Gullón, Alejandra Cardelle-Cobas, Freni K Tavaria
AIMS: When skin dysbiosis occurs as a result of skin disorders, probiotics can act as modulators, restoring microbial balance. Several properties of selected probiotics were evaluated so that their topical application could be considered. METHODS AND RESULTS: Adhesion, antimicrobial, quorum sensing and antibiofilm assays were carried out with several probiotic strains and tested against selected skin pathogens. All tested strains displayed significant adhesion to keratin...
November 12, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27856271/antimicrobial-antitrypanosomal-and-antibiofilm-activity-of-equisetum-hyemale
#5
Camilla Filippi Dos Santos Alves, Pauline Cordenonsi Bonez, Marcia de Ebling de Souza, Ritiel Corrêa da Cruz, Aline Augusti Boligon, Mariana Piana, Thiele Facim Brum, Grazielle Guidolin Rossi, Roberta da Silva Jesus, Thirssa Helena Grando, Silvia Gonzalez Monteiro, Marli Matiko Anraku de Campos, Janice Luering Giongo, Roberto Christ Vianna Santos
This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52...
November 14, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27836761/effect-of-holarrhena-antidysentrica-ha-and-andrographis-paniculata-ap-on-the-biofilm-formation-and-cell-membrane-integrity-of-opportunistic-pathogen-salmonella-typhimurium
#6
Ankit Tanwar, Raman Chawla, Ankita Singh Chakotiya, Pallavi Thakur, Rajeev Goel, Mitra Basu, Rajesh Arora, Haider Ali Khan
Increasing occurrence of gastroenteritis outbreaks caused by food borne opportunistic microorganisms has become a major problem in food industry as well as in immunocompromised host. Antimicrobial agents are losing their efficacy due to increase in the microbial resistance. For such reasons, conventional treatment has become limited to manage the infections state. Need of the hour is to instigate the search for safer holistic alternatives. The present study was hence conducted to assess the antibiofilm effect and mode of action of aquo alcoholic extracts of Holarrhena antidysentrica (Ha) and Andrographis paniculata (Ap) against the Salmonella enterica serovar typhimurium...
November 9, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27815202/haemolytic-and-antibiofilm-properties-of-haemocyanin-purified-from-the-haemolymph-of-indian-white-shrimp-fenneropenaeus-indicus
#7
Ramachandran Ishwarya, Baskaralingam Vaseeharan, Arokiadhas Iswarya, Sivashanmugam Karthikeyan
Haemocyanin (Hc) is an important non-specific immune macromolecule present in the haemolymph of both mollusks and crustaceans. In the present study, Hc was purified from the haemolymph of Indian white shrimp Fenneropenaeus indicus by gel filtration chromatography and it exhibits a single band with a molecular weight of 74 kDa on SDS-PAGE. The X-ray diffraction (XRD) and High performance liquid chromatography (HPLC) result of purified Hc express single peak at 31.5° be a sign of crystalline nature and appear as a single peak with a retention time of 5...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27812802/biotechnological-potential-of-actinobacteria-from-canadian-and-azorean-volcanic-caves
#8
Cristina Riquelme, Maria de Lurdes Enes Dapkevicius, Ana Z Miller, Zachary Charlop-Powers, Sean Brady, Cohord Mason, Naowarat Cheeptham
Caves are regarded as extreme habitats with appropriate conditions for the development of Actinobacteria. In comparison with other habitats, caves have not yet been the target of intensive screening for bioactive secondary metabolites produced by actinomycetes. As a primary screening strategy, we conducted a metagenomic analysis of the diversity and richness of a key gene required for non-ribosomal peptide (NRP) biosynthesis, focusing on cave-derived sediments from two Canadian caves (a lava tube and a limestone cave) to help us predict whether different types of caves may harbor drug-producing actinobacteria...
November 3, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27809517/antibacterial-and-antibiofilm-activity-of-cationic-small-molecules-with-spatial-positioning-of-hydrophobicity-an-in-vitro-and-in-vivo-evaluation
#9
Jiaul Hoque, Mohini Mohan Konai, Shanola Smitha Sequeira, Sandip Samaddar, Jayanta Haldar
More than 80% of the bacterial infections are associated with biofilm formation. To combat infections, amphiphilic small molecules have been developed as promising antibiofilm agents. However, cytotoxicity of such molecules still remains a major problem. Herein we demonstrate a concept in which antibacterial versus cytotoxic activities of cationic small molecules is tuned by spatial positioning of hydrophobic moieties while keeping positive charges constant. Compared to the molecules with more pendent hydrophobicity from positive centres (MIC = 1-4 μg/mL and HC50 = 60-65 μg/mL), molecules with more confined hydrophobicity between two centres show similar antibacterial activity but significantly less toxicity towards human erythrocytes (MIC = 1-4 μg/mL and HC50 = 805-1242 μg/mL)...
November 4, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27808174/essential-oils-and-eugenols-inhibit-biofilm-formation-and-the-virulence-of-escherichia-coli-o157-h7
#10
Yong-Guy Kim, Jin-Hyung Lee, Giyeon Gwon, Soon-Il Kim, Jae Gyu Park, Jintae Lee
Enterohemorrhagic Escherichia coli O157:H7 (EHEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. We investigated the abilities of various plant essential oils and their components to inhibit biofilm formation by EHEC. Bay, clove, pimento berry oils and their major common constituent eugenol at 0.005% (v/v) were found to markedly inhibit EHEC biofilm formation without affecting planktonic cell growth. In addition, three other eugenol derivatives isoeugenol, 2-methoxy-4-propylphenol, and 4-ethylguaiacol had antibiofilm activity, indicating that the C-1 hydroxyl unit, the C-2 methoxy unit, and C-4 alkyl or alkane chain on the benzene ring of eugenol play important roles in antibiofilm activity...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27787755/antimicrobial-peptide-isolated-from-bacillus-amyloliquefaciens-k14-revitalizes-its-use-in-combinatorial-drug-therapy
#11
Sudip Regmi, Yun Hee Choi, Yoon Seok Choi, Mi Ri Kim, Jin Cheol Yoo
The present study was performed to evaluate the antibacterial activities of an antimicrobial peptide (CSpK14) and the synergies thereof with β-lactams against vancomycin-resistant Staphylococcus aureus (VRSA) and Enterococci (VRE). Our strain was isolated from fermented food (kimchi), which is 99.79 % homologous with Bacillus amyloliquefaciens subsp. plantarum FZB42(T). CSpK14 was purified to homogeneity by diammonium sulfate precipitation, concentration, dialysis, and followed by two-stage chromatographic separation, i...
October 27, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27766027/capping-agent-dependent-toxicity-and-antimicrobial-activity-of-silver-nanoparticles-an-in-vitro-study-concerns-about-potential-application-in-dental-practice
#12
Karolina Niska, Narcyz Knap, Anna Kędzia, Maciej Jaskiewicz, Wojciech Kamysz, Iwona Inkielewicz-Stepniak
Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide antimicrobial activity spectrum. However, controversial information on AgNPs toxicity limited their use in oral infections. Therefore, the aim of the present study was to evaluate the antibacterial activities against a panel of oral pathogenic bacteria and bacterial biofilms together with potential cytotoxic effects on human gingival fibroblasts of 10 nm AgNPs: non-functionalized - uncapped (AgNPs-UC) as well as surface-functionalized with capping agent: lipoic acid (AgNPs-LA), polyethylene glycol (AgNPs-PEG) or tannic acid (AgNPs-TA) using silver nitrate (AgNO3) as control...
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27765621/green-synthesis-of-silver-gold-and-silver-gold-bimetallic-nanoparticles-using-the-gloriosa-superba-leaf-extract-and-their-antibacterial-and-antibiofilm-activities
#13
Kasi Gopinath, Shanmugasundaram Kumaraguru, Kasi Bhakyaraj, Subramanian Mohan, Kunga Sukumaran Venkatesh, Masanam Esakkirajan, Periyannan Kaleeswarran, Naiyf S Alharbi, Shine Kadaikunnan, Marimuthu Govindarajan, Giovanni Benelli, Ayyakannu Arumugam
The green fabrication of metal nanoparticles using botanical extracts is gaining increasing research attention in nanotechnology, since it does not require high energy inputs or the production of highly toxic chemical byproducts. Here, silver (Ag), gold (Au) and their bimetallic (Ag/Au) nanoparticles (NPs) were green synthesized using the Gloriosa superba aqueous leaf extract. Metal NPs were studied by spectroscopic (UV-visible spectroscopy, fluorescence spectroscopy, FT-IR spectroscopy, XRD and EDX) and microscopic (AFM and TEM) analysis...
October 17, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27759365/iron-complex-facilitated-copper-redox-cycling-for-nitric-oxide-generation-as-non-toxic-nitrifying-biofilm-inhibitor
#14
Vita Wonoputri, Cindy Gunawan, Sanly Liu, Nicolas Barraud, Lachlan Hartley Yee, May Lim, Rose Amal
In this study, we developed a poly(vinyl chloride) (PVC)-solvent casted mixed metal copper and iron complexes capable of catalytic generation of the antibiofilm nitric oxide (NO) from endogenous nitrite. In the absence of additional reducing agent, we demonstrated that the presence of iron complex facilitates a redox cycling, converting the copper(II) complex to active copper(I) species, which catalyzes the generation of NO from nitrite. Assessed by protein assay and surface coverage analyses, the presence of the mixed metal complexes in systems containing water industry-relevant nitrite-producing nitrifying biofilms was shown to result in a 'non-toxic mode' of biofilm suppression, while confining the bacterial growth to the free-floating planktonic phase...
October 19, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27757704/betulin-inhibits-cariogenic-properties-of-streptococcus-mutans-by-targeting-vicrk-and-gtf-genes
#15
Dharmaprakash Viszwapriya, Ganapathy Ashwinkumar Subramenium, Solai Radhika, Shunmugiah Karutha Pandian
Streptococcus mutans, a multivirulent pathogen is considered the primary etiological agent in dental caries. Development of antibiotic resistance in the pathogen has created a need for novel antagonistic agents which can control the virulence of the organism and reduce resistance development. The present study demonstrates the in vitro anti-virulence potential of betulin (lup-20(29)-ene-3β,28-diol), an abundantly available plant triterpenoid against S. mutans UA159. Betulin exhibited significant dose dependent antibiofilm activity without affecting bacterial viability...
October 18, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27725283/chitosanase-purified-from-bacterial-isolate-bacillus-licheniformis-of-ruined-vegetables-displays-broad-spectrum-biofilm-inhibition
#16
Sahira Nsayef Muslim, Israa M S Al-Kadmy, Nadheema Hammood Hussein, Alaa Naseer Mohammed Ali, Buthainah Mohammed Taha, Sarah Naji Aziz, Abdulaziz Abdullah Al Kheraif, Darshan Devang Divakar, Ravikumar Ramakrishnaiah
A number of bacterial species produces chitosanases which has variety of applications because of its high biodegradability, non-toxicity and antimicrobial assets. In the present study chitosanase is purified from new bacterial species Bacillus licheniformis from spoiled vegetable. This novel strain of Bacillus licheniformis isolated from spoilt cucumber and pepper samples has the ability to produce the chitosanase enzyme when grown on chitosan substrate. Study also examined its antibiofilm properties against diverse bacterial species with biofilm forming ability...
October 8, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27722576/co-loaded-proteinase-k-thyme-oil-liposomes-for-inactivation-of-escherichia-coli-o157-h7-biofilms-on-cucumber
#17
Haiying Cui, Cuixia Ma, Lin Lin
E. coli O157:H7 is a bacterium frequently found on vegetable surfaces, such as cucumber, that can pose a significant threat to consumers. In the present work, proteinase K (PK) and thyme oil (TO) were used to inactivate E. coli O157:H7 biofilms. Both PK and TO were encapsulated in liposomes to improve their chemical stability and to allow for a controlled release. The optimal PK/TO-loaded liposomes (particle size of 170.4 nm, polydispersity index of 0.309, zeta potential of -29.8 mV and entrapment efficiency of 33...
September 14, 2016: Food & Function
https://www.readbyqxmd.com/read/27721053/piper-betle-and-its-bioactive-metabolite-phytol-mitigates-quorum-sensing-mediated-virulence-factors-and-biofilm-of-nosocomial-pathogen-serratia-marcescens-in-vitro
#18
Ramanathan Srinivasan, Kannan Rama Devi, Arunachalam Kannappan, Shunmugiah Karutha Pandian, Arumugam Veera Ravi
ETHNOPHARMACOLOGICAL RELEVANCE: Piper betle, a tropical creeper plant belongs to the family Piperaceae. The leaves of this plant have been well known for their therapeutic, religious and ceremonial value in South and Southeast Asia. It has also been reported to possess several biological activities including antimicrobial, antioxidant, antinociceptive, antidiabetic, insecticidal and gastroprotective activities and used as a common ingredient in indigenous medicines. In Indian system of ayurvedic medicine, P...
October 6, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27709890/a-biodegradable-polycationic-paint-that-kills-bacteria-in-vitro-and-in-vivo
#19
Jiaul Hoque, Padma Akkapeddi, Chandradhish Ghosh, Divakara S S M Uppu, Jayanta Haldar
Bacterial colonization and subsequent formation of biofilms onto surfaces of medical devices and implants is a major source of nosocomial infections. Most antibacterial coatings to combat infections are either metal-based or nondegradable-polymer-based and hence limited by their nondegradability and unpredictable toxicity. Moreover, to combat infections effectively, the coatings are required to display simultaneous antibacterial and antibiofilm activity. Herein we report biocompatible and biodegradable coatings based on organo-soluble quaternary chitin polymers which were immobilized noncovalently onto surfaces as bactericidal paint...
November 2, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27702795/prevention-of-polymicrobial-biofilms-composed-of-pseudomonas-aeruginosa-and-pathogenic-fungi-by-essential-oils-from-selected-citrus-species
#20
Marina Pekmezovic, Ivana Aleksic, Aleksandra Barac, Valentina Arsic-Arsenijevic, Branka Vasiljevic, Jasmina Nikodinovic-Runic, Lidija Senerovic
Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L(-1), whereas no effect on P. aeruginosa growth was observed...
November 2016: Pathogens and Disease
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