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persister cells in biofilm

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https://www.readbyqxmd.com/read/28169510/differences-in-internalization-and-growth-of-escherichia-coli-o157-h7-within-the-apoplast-of-edible-plants-spinach-and-lettuce-compared-with-the-model-species-nicotiana-benthamiana
#1
Kathryn M Wright, Louise Crozier, Jacqueline Marshall, Bernhard Merget, Ashleigh Holmes, Nicola J Holden
Internalization of food-borne bacteria into edible parts of fresh produce plants represents a serious health risk. Therefore, internalization of verocytotoxigenic E. coli O157:H7 isolate Sakai was assessed in two species associated with outbreaks, spinach (Spinacia oleracea) and lettuce (Lactuca sativa) and compared to the model species Nicotiana benthamiana. Internalization occurred in the leaves and roots of spinach and lettuce throughout a 10 day time-course. The plant species, tissue type and inoculum dose all impacted the outcome...
February 7, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28167931/the-active-component-of-aspirin-salicylic-acid-promotes-staphylococcus-aureus-biofilm-formation-in-a-pia-dependent-manner
#2
Cristian Dotto, Andrea Lombarte Serrat, Natalia Cattelan, María S Barbagelata, Osvaldo M Yantorno, Daniel O Sordelli, Monika Ehling-Schulz, Tom Grunert, Fernanda R Buzzola
Aspirin has provided clear benefits to human health. But salicylic acid (SAL) -the main aspirin biometabolite- exerts several effects on eukaryote and prokaryote cells. SAL can affect, for instance, the expression of Staphylococcus aureus virulence factors. SAL can also form complexes with iron cations and it has been shown that different iron chelating molecules diminished the formation of S. aureus biofilm. The aim of this study was to elucidate whether the iron content limitation caused by SAL can modify the S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28167629/il-10-plays-opposing-roles-during-staphylococcus-aureus-systemic-and-localized-infections
#3
John M Leech, Keenan A Lacey, Michelle E Mulcahy, Eva Medina, Rachel M McLoughlin
IL-10 is a potent anti-inflammatory mediator that plays a crucial role in limiting host immunopathology during bacterial infections by controlling effector T cell activation. Staphylococcus aureus has previously been shown to manipulate the IL-10 response as a mechanism of immune evasion during chronic systemic and biofilm models of infection. In the present study, we demonstrate divergent roles for IL-10 depending on the site of infection. During acute systemic S. aureus infection, IL-10 plays an important protective role and is required to prevent bacterial dissemination and host morbidity by controlling effector T cells and the associated downstream hyperactivation of inflammatory phagocytes, which are capable of host tissue damage...
February 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28159787/biofilm-formation-on-stainless-steel-in-milk-environment-is-mediated-by-the-proteinase-prts-in-streptococcus-thermophilus-uc8547
#4
D Bassi, F Cappa, S Gazzola, L Orrù, P S Cocconcelli
: In Streptococcus thermophilus gene transfer events and loss of ancestral traits during years contribute to its high adaptation to the milk environment. Biofilm formation capacity, a phenotype that is lost in the majority of strains, plays a role in the persistence in dairy environments, such as milk pasteurisation and cheese manufacturing plants. To investigate on this property, we have studied S. thermophilus UC8547, a fast acidifying dairy starter culture, selected for its high capacity to form biofilm on stainless steel in environmental conditions resembling the dairy environment...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28150898/biofilm-formation-invasiveness-and-colicinogeny-in-locus-of-enterocyte-and-effacement-negative-o113-h21-shigatoxigenic-escherichia-coli
#5
Luís Fernando Dos Santos, Franciele Tafarello Biscola, Evanilde Maria Gonçalves, Beatriz Ernestina Cabílio Guth
AIMS: Although Shiga toxins (Stx) are well established virulence traits of O113:H21 Shigatoxigenic Escherichia coli (STEC) strains, a shortage in the knowledge of other virulence properties that may contribute to pathogenesis may exist in this serotype. This study investigated biofilm, invasiveness and colicinogeny capabilities in O113:H21 STEC isolated in Brazil, mostly from animal reservoirs. A search for genes which were reported to participate in the process of biofilm formation was also performed...
February 2, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28138928/analysis-of-pseudomonas-aeruginosa-pao1-biofilm-protein-profile-after-exposure-to-n-butanolic-cyclamen-coum-extract-alone-and-in-combination-with-ciprofloxacin
#6
Morvarid Shafiei, Ahya Abdi-Ali, Fereshteh Shahcheraghi, Hojatollah Vali, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi
Pseudomonas aeruginosa biofilm-related infections are the major cause of premature death in cystic fibrosis patients. Strategies to induce biofilm dispersal are of interest, because of their potential in preventing biofilm-related infections. Our previous work demonstrated that n-butanolic Cyclamen coum extract with ciprofloxacin could eliminate 1- and 3-day-old P. aeruginosa PAO1 biofilms. To gain new insights into the role of C. coum extract and its synergistic effect with ciprofloxacin in eliminating P. aeruginosa PAO1 biofilms, two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry-based protein identification were used...
January 30, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28132897/targeting-biofilms-and-persisters-of-eskape-pathogens-with-p14kans-a-kanamycin-peptide-conjugate
#7
Mohamed F Mohamed, Anna Brezden, Haroon Mohammad, Jean Chmielewski, Mohamed N Seleem
BACKGROUND: The worldwide emergence of antibiotic resistance represents a serious medical threat. The ability of these resistant pathogens to form biofilms that are highly tolerant to antibiotics further aggravates the situation and leads to recurring infections. Thus, new therapeutic approaches that adopt novel mechanisms of action are urgently needed. To address this significant problem, we conjugated the antibiotic kanamycin with a novel antimicrobial peptide (P14LRR) to develop a kanamycin peptide conjugate (P14KanS)...
January 27, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28115132/characterization-of-bacterial-community-dynamics-in-a-full-scale-drinking-water-treatment-plant
#8
Cuiping Li, Fangqiong Ling, Minglu Zhang, Wen-Tso Liu, Yuxian Li, Wenjun Liu
Understanding the spatial and temporal dynamics of microbial communities in drinking water systems is vital to securing the microbial safety of drinking water. The objective of this study was to comprehensively characterize the dynamics of microbial biomass and bacterial communities at each step of a full-scale drinking water treatment plant in Beijing, China. Both bulk water and biofilm samples on granular activated carbon (GAC) were collected over 9months. The proportion of cultivable cells decreased during the treatment processes, and this proportion was higher in warm season than cool season, suggesting that treatment processes and water temperature probably had considerable impact on the R2A cultivability of total bacteria...
January 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28112955/fate-of-eight-different-polymers-under-uncontrolled-composting-conditions-relationships-between-deterioration-biofilm-formation-and-the-material-surface-properties
#9
Anne Mercier, Kevin Gravouil, Willy Aucher, Sandra Brosset-Vincent, Linette Kadri, Jenny Colas, Didier Bouchon, Thierry Ferreira
With the ever-increasing volume of polymer wastes and their associated detrimental impacts on the environment, the plastic life cycle has drawn increasing attention. Here, eight commercial polymers selected from biodegradable to environmentally persistent materials, all formulated under a credit card format, were incubated in an outdoor compost to evaluate their fate over time and to profile the microbial communities colonizing their surfaces. After 450 days in compost, the samples were all colonized by multispecies biofilms, these latest displaying different amounts of adhered microbial biomass and significantly distinct bacterial and fungal community compositions depending on the substrate...
February 8, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28112760/the-anti-inflammatory-drug-mesalamine-targets-bacterial-polyphosphate-accumulation
#10
Jan-Ulrik Dahl, Michael J Gray, Daphne Bazopoulou, Francois Beaufay, Justine Lempart, Mark J Koenigsknecht, Ying Wang, Jason R Baker, William L Hasler, Vincent B Young, Duxin Sun, Ursula Jakob
Mesalamine serves as the gold standard in treating ulcerative colitis. However, its precise mechanism(s) of action remains unclear. Here, we show that mesalamine treatment rapidly decreases polyphosphate levels in diverse bacteria, including members of the human gut microbiome. This decrease sensitizes bacteria towards oxidative stress, reduces colonization and attenuates persister cell and biofilm formation, suggesting that mesalamine aids in diminishing the capacity of bacteria to persist within chronically inflamed environments...
January 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28111984/effects-of-meropenem-exposure-in-persister-cells-of-acinetobacter-calcoaceticus-baumannii
#11
Stephanie Wagner Gallo, Bruna Kern Donamore, Vany Elisa Pagnussatti, Carlos Alexandre Sanchez Ferreira, Sílvia Dias de Oliveira
AIM: To evaluate the influence of meropenem in the Acinetobacter calcoaceticus-baumannii (ACB) persister levels. METHODS: Persister levels in planktonic and biofilm cultures from ACB isolates were evaluated after exposure to different meropenem concentrations. RESULTS: A high variability of persister fractions was observed among the isolates cultured under planktonic and biofilm conditions. Meropenem concentration did not influence persister fractions, even when far above the MIC...
January 23, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28110918/nitroxoline-a-broad-spectrum-biofilm-eradicating-agent-against-pathogenic-bacteria
#12
Yasmeen Abouelhassan, Qingping Yang, Hussain Yousaf, Minh Thu Nguyen, Melanie Rolfe, Gregory S Schultz, Robert W Huigens
Bacterial biofilms are surface-attached communities of slow-growing or non-replicating bacteria tolerant to conventional antibiotic therapies. Although biofilms are known to occur in ca. 80% of all bacterial infections, no therapeutic agent has been developed to eradicate bacteria housed within biofilms. We have discovered that nitroxoline, an antibacterial agent used to treat urinary tract infections, displays broad-spectrum biofilm-eradicating activities against major human pathogens, including drug-resistant Staphylococcus aureus and Acinetobacter baumannii strains...
December 6, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28073080/impact-of-environmental-factors-on-the-culturability-and-viability-of-listeria-monocytogenes-under-conditions-encountered-in-food-processing-plants
#13
Anaïs Overney, Joséphine Jacques-André-Coquin, Patricia Ng, Brigitte Carpentier, Laurent Guillier, Olivier Firmesse
The ability of Listeria monocytogenes to adhere to and persist on surfaces for months or even years may be responsible for its transmission from contaminated surfaces to food products. Hence the necessity to find effective means to prevent the establishment of L. monocytogenes in food processing environments. The aim of this study was to assess, through a fractional experimental design, the environmental factors that could affect the survival of L. monocytogenes cells on surfaces to thereby prevent the persistence of this pathogen in conditions mimicking those encountered in food processing plants: culture with smoked salmon juice or meat exudate, use of two materials with different hygiene status, biofilm of L...
March 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28049020/eradication-of-pseudomonas-aeruginosa-cells-by-cathodic-electrochemical-currents-delivered-with-graphite-electrodes
#14
Tagbo H R Niepa, Hao Wang, Jeremy L Gilbert, Dacheng Ren
: Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we reported a new approach to eradicating persister cells of Pseudomonas aeruginosa using low-level direct electrochemical current (DC) and synergy with the antibiotic tobramycin...
December 31, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28046077/an-oxygen-sensing-two-component-system-in-the-burkholderia-cepacia-complex-regulates-biofilm-intracellular-invasion-and-pathogenicity
#15
Matthew M Schaefers, Tiffany L Liao, Nicole M Boisvert, Damien Roux, Deborah Yoder-Himes, Gregory P Priebe
Burkholderia dolosa is a member of the Burkholderia cepacia complex (BCC), which is a group of bacteria that cause chronic lung infection in patients with cystic fibrosis (CF) and can be associated with outbreaks carrying high morbidity and mortality. While investigating the genomic diversity of B. dolosa strains collected from an outbreak among CF patients, we previously identified fixL as a gene showing signs of strong positive selection. This gene has homology to fixL of the rhizobial FixL/FixJ two-component system...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28043500/design-and-development-of-novel-inhibitors-for-the-treatment-of-latent-tuberculosis
#16
Rudraraju Srilakshmi Reshma, Perumal Yogeeswari, Dharmarajan Sriram
OBJECTIVE/BACKGROUND: "The captain of all these men of death", is the apt sobriquet for the age-old disease tuberculosis (TB). Despite the availability of many drugs, cases of increasing resistance in the forms of multi-drug and extensively drug-resistant TB and persistence [characteristic of Mycobacterium tuberculosis (MTB)] make the eradication of TB a nightmare. Approval of bedaquiline by the Food and Drug Administration focused attention on quinoline scaffolds for development of new anti-TB agents...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28031194/azithromycin-ciprofloxacin-impregnated-urinary-catheters-avert-bacterial-colonization-biofilm-formation-and-inflammation-in-a-murine-model-of-foreign-body-associated-urinary-tract-infections-caused-by-pseudomonas-aeruginosa
#17
Hina Saini, Anitha Vadekeetil, Sanjay Chhibber, Kusum Harjai
Pseudomonas aeruginosa is a multifaceted pathogen causing a variety of biofilm-mediated infections including catheter-associated urinary tract infections (CAUTIs). High prevalence of CAUTIs in hospitals, their clinical manifestations like urethritis, cystitis, pyelonephritis, meningitis, urosepsis, death; and economic challenges underscore the need for management of these infections. Biomaterial modification of urinary catheters with dual drugs seems an interesting approach to combat CAUTIs by inhibiting biofilm...
December 28, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28026958/human-%C3%AE-defensin-6-self-assembly-prevents-adhesion-and-suppresses-virulence-traits-of-candida-albicans
#18
Phoom Chairatana, I-Ling Chiang, Elizabeth M Nolan
HD6 is a host-defense peptide that contributes to intestinal innate immunity and mediates homeostasis at mucosal surfaces by forming non-covalent oligomers that capture bacteria and prevent bacterial invasion into the epithelium. Our present work illustrates a new role of HD6 in defending the host epithelium against pathogenic microorganisms. We report that HD6 blocks Candida albicans adhesion to human intestinal epithelial cells and suppresses two C. albicans virulence traits, namely invasion into human epithelial cells and biofilm formation...
December 27, 2016: Biochemistry
https://www.readbyqxmd.com/read/28019691/biofilm-detection-by-wound-blotting-can-predict-slough-development-in-pressure-ulcers-a-prospective-observational-study
#19
Gojiro Nakagami, Gregory Schultz, Daniel J Gibson, Priscilla Phillips, Aya Kitamura, Takeo Minematsu, Tomomitsu Miyagaki, Akitatsu Hayashi, Sanae Sasaki, Junko Sugama, Hiromi Sanada
Bacteria have been found to form multicellular aggregates which have collectively been termed "biofilms". It is hypothesized that biofilm formation is a means to protect bacterial cells including protection form the immune response of humans. This protective mechanism is believed to explain persistent chronic wound infections. At times, the biofilms are abundant enough to see, and remove by simple wiping. However, recent evidence has shown that the removal of these visible portions are not sufficient, and that biofilms can continue to form even with daily wiping...
December 26, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27995274/emergence-of-life-on-earth-a-physicochemical-jigsaw-puzzle
#20
REVIEW
Jan Spitzer
We review physicochemical factors and processes that describe how cellular life can emerge from prebiotic chemical matter; they are: (1) prebiotic Earth is a multicomponent and multiphase reservoir of chemical compounds, to which (2) Earth-Moon rotations deliver two kinds of regular cycling energies: diurnal electromagnetic radiation and seawater tides. (3) Emerging colloidal phases cyclically nucleate and agglomerate in seawater and consolidate as geochemical sediments in tidal zones, creating a matrix of microspaces...
January 2017: Journal of Molecular Evolution
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