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Wenjun Zhang, Junfang Chen, Xiao Hong Pan, Jiafeng Zhang, Zhihong Guo, Yan Luo, Jiezhe Yang, Yan Xia, Lin He, Yun Xu, Ke Xu, Xiaobei Ding
OBJECTIVE: To investigate the HIV-1 molecular epidemiology among young people (18 to 25 years old) in Hangzhou. METHODS: Plasma samples from 262 newly diagnosed HIV-1 infected patients were collected between 2009 and 2013 from Hangzhou of Zhejiang province. HIV-1 nucleotide sequences of pol gene regions were amplified using a nested PCR method and sequenced. Phylogenetic and recombination analyses were used to determine the HIV-1 genotypes. RESULTS: Based on all sequences generated, the subtype/circulating recombinant forms (CRF) distribution was as follows: CRF01_AE (68...
October 20, 2016: AIDS Research and Human Retroviruses
Yihong Hu, Zhenzhou Wan, Yan-Heng Zhou, Davey M Smith, Yong-Tang Zheng, Chiyu Zhang
The on-going generation of HIV-1 inter-subtype recombination has led to new circulating recombinant forms (CRFs) and unique recombinant forms (URFs) in Asia. Here, we evaluated whether previously reported URFs were actually CRFs. All available complete or near full-length HIV-1 URFs sequences from Asia were retrieved from the HIV Los Alamos National Laboratory Sequence database, and phylogenetic, transmission cluster and bootscan analyses were performed using MEGA 6.0, Cluster Picker 1.2.1, and SimPlot3.5.1...
October 20, 2016: AIDS Research and Human Retroviruses
Mara Cucinotta, Silvia Manrique, Andrea Guazzotti, Nadia E Quadrelli, Marta A Mendes, Eva Benkova, Lucia Colombo
The developmental program of the pistil is under the control of both auxin and cytokinin, which crosstalk converges on the regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes couldn't be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present...
October 13, 2016: Development
Giuseppina Musumeci, Giacomo Magnani, Isabella Bon, Serena Longo, Alessia Bertoldi, Anna Maria Degli Antoni, Maria Rita Rossi, Alessandro Ruggeri, Vittorio Sambri, Simona Semprini, Laura Sighinolfi, Maria Alessandra Ursitti, Alessandro Zerbini, Vincenzo Colangeli, Leonardo Calza, Alba Carola Finarelli, Erika Massimiliani, Maria Carla Re
It is crucial to establish the timing of infection and distinguish between early and long-lasting HIV-1 infections not only for partner notification and epidemiological surveillance, but also to offer early drug treatment and contain the spread of infection. This study analyzed serum and/or plasma samples with a first positive HIV antibody/antigen result coming from different Medical Centers in the Emilia Romagna Region, North East Italy, using the avidity assay, Western Blotting, RNA viral load, CD4 cell counts and genotyping assay...
September 13, 2016: New Microbiologica
Paul Talman, Thi Duong, Steve Vucic, Susan Mathers, Svetha Venkatesh, Robert Henderson, Dominic Rowe, David Schultz, Robert Edis, Merrilee Needham, Richard Macdonnell, Pamela McCombe, Carol Birks, Matthew Kiernan
OBJECTIVE: To capture the clinical patterns, timing of key milestones and survival of patients presenting with amyotrophic lateral sclerosis/motor neuron disease (ALS/MND) within Australia. METHODS: Data were prospectively collected and were timed to normal clinical assessments. An initial registration clinical report form (CRF) and subsequent ongoing assessment CRFs were submitted with a completion CRF at the time of death. DESIGN: Prospective observational cohort study...
September 30, 2016: BMJ Open
Monica Rydén-Aulin, Dimitrios Boumpas, Irene Bultink, Jose Luis Callejas Rubio, Luis Caminal-Montero, Antoni Castro, Agustín Colodro Ruiz, Andrea Doria, Thomas Dörner, Cristina Gonzalez-Echavarri, Elisa Gremese, Frederic A Houssiau, Tom Huizinga, Murat Inanç, David Isenberg, Annamaria Iuliano, Søren Jacobsen, Juan Jimenéz-Alonso, Lászlo Kovács, Xavier Mariette, Marta Mosca, Ola Nived, Joaquim Oristrell, Manuel Ramos-Casals, Javier Rascón, Guillermo Ruiz-Irastorza, Luis Sáez-Comet, Gonzalo Salvador Cervelló, Gian Domenico Sebastiani, Danilo Squatrito, Gabriella Szücs, Alexandre Voskuyl, Ronald van Vollenhoven
OBJECTIVES: Rituximab (RTX) is a biological treatment used off-label in patients with systemic lupus erythematosus (SLE). This survey aimed to investigate the off-label use of RTX in Europe and compare the characteristics of patients receiving RTX with those receiving conventional therapy. METHODS: Data on patients with SLE receiving RTX were taken from the International Registry for Biologics in SLE retrospective registry and complemented with data on patients with SLE treated with conventional therapy...
2016: Lupus Science & Medicine
Danqing Hu, Zhengxing Huang, Tak-Ming Chan, Wei Dong, Xudong Lu, Huilong Duan
Background: Clinical major adverse cardiovascular event (MACE) prediction of acute coronary syndrome (ACS) is important for a number of applications including physician decision support, quality of care assessment, and efficient healthcare service delivery on ACS patients. Admission records, as typical media to contain clinical information of patients at the early stage of their hospitalizations, provide significant potential to be explored for MACE prediction in a proactive manner. Methods: We propose a hybrid approach for MACE prediction by utilizing a large volume of admission records...
2016: International Journal of Environmental Research and Public Health
Ben G L Vanneste, Aswin L Hoffmann, Emile N van Lin, Lien Van De Voorde, Michael Pinkawa, Philippe Lambin
BACKGROUND AND PURPOSE: Previous studies confirmed that implantable rectum spacers (IRS) decreased acute gastro-intestinal (GI) toxicity in a significant percentage of prostate cancer patients undergoing intensity modulated radiation therapy (IMRT). We developed decision rules based on clinical risk factors (CRFs) to select those patients who are expected to benefit most from IRS implantation. MATERIALS AND METHODS: For 26 patients dose distributions with (IMRT+IRS) and without (IMRT-IRS) IRS were calculated...
September 16, 2016: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
Doğu Ömür Dede, Onur Sahin, Oğuz Süleyman Özdemir, Burak Yilmaz, Ersan Celik, AySegül Köroğlu
STATEMENT OF PROBLEM: Lithium disilicate restorations are commonly used, particularly in the anterior region. The color of the underlying composite resin foundation (CRF) and luting cement may negatively affect the color of lithium disilicate ceramic restorations. PURPOSE: The purpose of this in vitro study was to investigate the effect of CRF and resin cement materials on the color of lithium disilicate ceramics in 2 different translucencies. MATERIAL AND METHODS: Twenty disks (11×1...
September 9, 2016: Journal of Prosthetic Dentistry
Javier Valero-Elizondo, Joseph A Salami, Chukwuemeka U Osondu, Oluseye Ogunmoroti, Alejandro Arrieta, Erica S Spatz, Adnan Younus, Jamal S Rana, Salim S Virani, Ron Blankstein, Michael J Blaha, Emir Veledar, Khurram Nasir
BACKGROUND: Physical activity (PA) has an established favorable impact on cardiovascular disease (CVD) outcomes and quality of life. In this study, we aimed to estimate the economic effect of moderate-vigorous PA on medical expenditures and utilization from a nationally representative cohort with and without CVD. METHODS AND RESULTS: The 2012 Medical Expenditure Panel Survey data were analyzed. Our study population was limited to noninstitutionalized US adults ≥18 years of age...
2016: Journal of the American Heart Association
Annelies Van Hemelryk, Gert De Meerleer, Piet Ost, Filip Poelaert, Werner De Gersem, Karel Decaestecker, Pieter De Visschere, Valérie Fonteyne
PURPOSE: Improved outcome is reported after surgery or external beam radiation therapy (EBRT) plus androgen deprivation therapy (ADT) for patients with lymph node (LN) positive (N1) prostate cancer (PC). Surgical series have shown that pathologic (p)N1 PC does not behave the same in all patients. The aim of this study was to perform a matched-case analysis to compare the outcome of pN1 and pN0 PC after high-dose EBRT plus ADT. METHODS AND MATERIALS: Radiation therapy up to 80 Gy was delivered to the prostate with a minimal dose of 45 Gy to the pelvis for pN1 patients...
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
Yanping Li, Jing Miao, Zhijiang Miao, Yindi Song, Min Wen, Ya Zhang, Shimin Guo, Yuan Zhao, Yue Feng, Xueshan Xia
In recent years, multiple circulating recombinant forms (CRFs) and unique recombinant forms of human immunodeficiency virus type 1 (HIV-1) have been described in Yunnan, China. Here, we identified a novel HIV-1 CRF (CRF86_BC) isolated from three heterosexuals with no obvious epidemiologic linkage in western Yunnan (Baoshan prefecture) in China. CRF86_BC had a subtype C backbone with four subtype B fragments inserted into the pol, vpr, vpu, env, and nef gene regions, respectively. Furthermore, subregion tree analysis revealed that subtype C backbone originated from an Indian C lineage and subtype B segment inserted was from a Thai B lineage...
October 18, 2016: AIDS Research and Human Retroviruses
Yasushi Matsumura, Atsushi Hattori, Shiro Manabe, Tsutomu Tsuda, Toshihiro Takeda, Katsuki Okada, Taizo Murata, Naoki Mihara
There is a great need to reuse data stored in electronic medical records (EMR) databases for clinical research. We previously reported the development of a system in which progress notes and case report forms (CRFs) were simultaneously recorded using a template in the EMR in order to exclude redundant data entry. To make the data collection process more efficient, we are developing a system in which the data originally stored in the EMR database can be populated within a frame in a template. We developed interface plugin modules that retrieve data from the databases of other EMR applications...
2016: Studies in Health Technology and Informatics
Wei Zhen Chow, Abdul Hamid Bon, Sheila Keating, Fread Anderios, Hazwan Abdul Halim, Yutaka Takebe, Adeeba Kamarulzaman, Michael P Busch, Kok Keng Tee
Transfusion-transmissible infections including HIV-1 continue to pose major risks for unsafe blood transfusions due to both window phase infections and divergent viruses that may not be detected by donor screening assays. Given the recent emergence of several HIV-1 circulating recombinant forms (CRFs) in high-risk populations in the Southeast Asia region, we investigated the genetic diversity of HIV-1 among the blood donors in Kuala Lumpur, Malaysia. A total of 211 HIV-positive plasma samples detected among 730,188 donations to the National Blood Centre between 2013 and 2014 were provided (90...
2016: PloS One
Katherine E Beaney, Jackie A Cooper, Stela McLachlan, S Goya Wannamethee, Barbara J Jefferis, Peter Whincup, Yoav Ben-Shlomo, Jacqueline F Price, Meena Kumari, Andrew Wong, Ken Ong, Rebecca Hardy, Diana Kuh, Mika Kivimaki, Antti J Kangas, Pasi Soininen, Mika Ala-Korpela, Fotios Drenos, Steve E Humphries
AIMS: An intergenic locus on chromosome 1 (lead SNP rs10911021) was previously associated with coronary heart disease (CHD) in type 2 diabetes (T2D). Using data from the UCLEB consortium we investigated the relationship between rs10911021 and CHD in T2D, whether rs10911021 was associated with levels of amino acids involved in the γ-glutamyl cycle or any conventional risk factors (CRFs) for CHD in the T2D participants. METHODS: Four UCLEB studies (n = 6531) had rs10911021 imputation, CHD in T2D, CRF and metabolomics data determined using a nuclear magnetic resonance based platform...
2016: Cardiovascular Diabetology
M N Zheng, T L Ning, Y J Gao, X Zhao, L Li, S H Cheng
OBJECTIVE: To understand the characteristics on the diversity of HIV subtypes and related transmission in Tianjin. METHODS: A total of 77 newly diagnosed ART-naïve HIV infectors in 2015 were screened by CD4(+) T cell counts ≥200 cells/μl. Viral RNAs were extracted from plasma samples. Reverse transcription PCR and nested PCR were used to analyze the amplification of pol and env gene fragments, sequencing, and related bioinformatics information. RESULTS: 46...
August 10, 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Dieu-My T Tran, Lani M Zimmerman, Kevin A Kupzyk
BACKGROUND AND PURPOSE: To test the psychometric properties of two measures of knowledge and perception of cardiovascular risk factors (CRFs). METHODS: A methodological study using a modified version of the Heart Disease Fact Questionnaire (HDFQ) and the Health Beliefs Related to Cardiovascular Disease (HBCVD) questionnaires was administered to 100 college-aged students. RESULTS: The HDFQ Kuder-Richardson-20 reliability estimate was .73. The HBCVD Cronbach's alpha was ...
2016: Journal of Nursing Measurement
Mark M Manak, Holly R Hack, Sangeetha V Nair, Andrew Worlock, Jennifer A Malia, Sheila A Peel, Linda L Jagodzinski
Quantitation of the HIV-1 viral load in plasma is the current standard of care for clinical monitoring of HIV-infected individuals undergoing antiretroviral therapy. This study evaluated the analytical and clinical performances of the Aptima HIV-1 Quant Dx assay (Hologic, San Diego, CA) for monitoring viral load by using 277 well-characterized subtype samples, including 171 cultured virus isolates and 106 plasma samples from 35 countries, representing all major HIV subtypes, recombinants, and circulating recombinant forms (CRFs) currently in circulation worldwide...
October 2016: Journal of Clinical Microbiology
Jitendra Jonnagaddala, Toni Rose Jue, Nai-Wen Chang, Hong-Jie Dai
The rapidly increasing biomedical literature calls for the need of an automatic approach in the recognition and normalization of disease mentions in order to increase the precision and effectivity of disease based information retrieval. A variety of methods have been proposed to deal with the problem of disease named entity recognition and normalization. Among all the proposed methods, conditional random fields (CRFs) and dictionary lookup method are widely used for named entity recognition and normalization respectively...
2016: Database: the Journal of Biological Databases and Curation
Na Hong, Dingcheng Li, Yue Yu, Qiongying Xiu, Hongfang Liu, Guoqian Jiang
BACKGROUND: Constructing standard and computable clinical diagnostic criteria is an important but challenging research field in the clinical informatics community. The Quality Data Model (QDM) is emerging as a promising information model for standardizing clinical diagnostic criteria. OBJECTIVE: To develop and evaluate automated methods for converting textual clinical diagnostic criteria in a structured format using QDM. METHODS: We used a clinical Natural Language Processing (NLP) tool known as cTAKES to detect sentences and annotate events in diagnostic criteria...
July 19, 2016: Journal of Biomedical Informatics
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