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https://www.readbyqxmd.com/read/28336844/gold-nanobeacons-for-tracking-gene-silencing-in-zebrafish
#1
Milton Cordeiro, Lara Carvalho, Joana Silva, Leonor Saúde, Alexandra R Fernandes, Pedro V Baptista
The use of gold nanoparticles for effective gene silencing has demonstrated its potential as a tool for gene expression experiments and for the treatment of several diseases. Here, we used a gold nanobeacon designed to specifically silence the enhanced green fluorescence protein (EGFP) mRNA in embryos of a fli-EGFP transgenic zebrafish line, while simultaneously allowing the tracking and localization of the silencing events via the beacon's emission. Fluorescence imaging measurements demonstrated a decrease of the EGFP emission with a concomitant increase in the fluorescence of the Au-nanobeacon...
January 11, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28323199/acute-toxicity-bioaccumulation-and-effects-of-dietary-transfer-of-silver-from-brine-shrimps-exposed-to-pvp-pei-coated-silver-nanoparticles-to-zebrafish
#2
José María Lacave, Álvaro Fanjul, Eider Bilbao, Nerea Gutierrez, Irantzu Barrio, Inmaculada Arostegui, Miren P Cajaraville, Amaia Orbea
The extensive use and release to the aquatic environment of silver nanoparticles (NPs) could lead to their incorporation into the food web. Brine shrimp larvae of 24h showed low sensitivity to the exposure to PVP/PEI-coated Ag NPs (5nm), with EC50 values at 24h of 19.63mg Ag L(-1), but they significantly accumulated silver after 24h of exposure to 100μg L(-1) of Ag NPs. Thus, to assess bioaccumulation and effects of silver transferred by the diet in zebrafish, brine shrimp larvae were exposed to 100ng L(-1) of Ag NPs as an environmentally relevant concentration or to 100μg L(-1) as a potentially effective concentration and used to feed zebrafish for 21days...
March 16, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28299721/pro-np%C3%A2-protect-against-tio2-nanoparticle-induced-phototoxicity-in-zebrafish-model-exploring-potential-application-for-skin-care
#3
Min-Sik Kim, Melinda Stees, Bala Vamsi K Karuturi, Sivakumar Vijayaraghavalu, Richard E Peterson, Gary L Madsen, Vinod Labhasetwar
Titanium dioxide nanoparticles (TiO2NPs) are used in sunscreen products to protect the skin from the sun's ultraviolet rays. However, following exposure to sunlight, the photocatalytic activity of TiO2NPs can produce an excess of reactive oxygen species (ROS), causing skin cell damage, triggering an inflammatory response. In zebrafish model, we evaluated how well Pro-NP™ (biodegradable NPs containing superoxide dismutase and catalase) could protect them from TiO2NP-induced photo-oxidative stress. We hypothesized that the antioxidant properties of Pro-NP™ would protect zebrafish embryos from the phototoxic effects of TiO2NPs, improving overall survival and growth...
March 15, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28289416/specific-and-efficient-uptake-of-surfactant-free-poly-lactic-acid-nanovaccine-vehicles-by-mucosal-dendritic-cells-in-adult-zebrafish-after-bath-immersion
#4
Julien Rességuier, Emilie Delaune, Anne-Line Coolen, Jean-Pierre Levraud, Pierre Boudinot, Dominique Le Guellec, Bernard Verrier
Activation of mucosal immunity is a key milestone for next-generation vaccine development. Biocompatible polymer-based nanoparticles (NPs) are promising vectors and adjuvants for mucosal vaccination. However, their in vivo uptake by mucosae and their biodistribution in antigen-presenting cells (APCs) need to be better understood to optimize mucosal nanovaccine designs. Here, we assessed if APCs are efficiently targeted in a spontaneous manner by surfactant-free poly(lactic acid) nanoparticles (PLA-NPs) after mucosal administration...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28286999/an-assessment-of-the-importance-ofexposure-routes-to-the-uptake-and-internal-localisation-of-fluorescent-nanoparticles-in-zebrafish-danio-rerio-using-light-sheet-microscopy
#5
L M Skjolding, G Ašmonaitė, R I Jølck, T L Andresen, H Selck, A Baun, J Sturve
A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some uptake methods have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used light sheet microscopy (LSM) to define the uptake and localisation of fluorescently labelled nanoparticles in living organisms with minimal sample preparation. Zebrafish (Danio rerio) were exposed to fluorescent gold nanoparticles (Au NPs) and fluorescent polystyrene NPs via aqueous or dietary exposure...
March 11, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28274763/developmental-and-reproductive-toxicity-of-pvp-pei-coated-silver-nanoparticles-to-zebrafish
#6
Amaia Orbea, Nagore González-Soto, José María Lacave, Irantzu Barrio, Miren P Cajaraville
Cellular and molecular mechanisms of toxicity of silver nanoparticles (NPs) and their toxicity to fish embryos after waterborne exposure have been widely investigated, but much less information is available regarding the effect of Ag NPs on physiological functions such as growth or reproduction. In this work, the effects of waterborne exposure of adult zebrafish (Danio rerio) to PVP/PEI coated Ag NPs (~5nm) on reproduction (fecundity) were investigated. Moreover, the development of the embryos after parental exposure was compared with the development of embryos after direct waterborne exposure to the NPs...
March 6, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28263053/water-soluble-fullerene-derivatives-as-brain-medicine-surface-chemistry-determines-if-they-are-neuroprotective-and-antitumor
#7
Fu-Yu Hsieh, A V Zhilenkov, I I Voronov, E A Khakina, D V Mischenko, Pavel A Troshin, Shan-Hui Hsu
Delivering drugs to the central nervous system (CNS) is a major challenge in treating CNS-related diseases. Nanoparticles that can cross blood-brain barrier (BBB) are potential tools. In this study, water-soluble C60 fullerene derivatives with different types of linkages between the fullerene cage and the solubilizing addend were synthesized (compounds 1-3: C-C bonds, compounds 4-5: C-S bonds, compound 6: C-P bonds, and compounds 7-9: C-N bonds). Fullerene derivatives 1-6 were observed to induce neural stem cell (NSC) proliferation in vitro and rescue the function of injured CNS in zebrafish...
March 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28247754/small-sized-mpeg-plga-nanoparticles-of-schisantherin-a-with-sustained-release-for-enhanced-brain-uptake-and-anti-parkinsonian-activity
#8
Tongkai Chen, Chuwen Li, Ye Li, Xiang Yi, Ruibing Wang, Simon Ming-Yuen Lee, Ying Zheng
Schisantherin A (SA) is a promising anti-Parkinsonism natural product. However, its poor water solubility and rapid serum clearance impose significant barriers to delivery of SA to the brain. This work aimed to develop SA in a nanoparticle formulation that extended SA circulation in the bloodstream and consequently an increased brain uptake and thus to be potentially efficacious for the treatment of Parkinson's disease (PD). Spherical SA nanoparticles with a mean particle size of 70 nm were prepared by encapsulating SA into methoxy poly(ethylene glycol)-block-poly(d,l)-lactic-co-glycolic acid (mPEG-PLGA) nanoparticles (SA-NPs) with an encapsulation efficiency of ∼91% and drug loading of ∼28%...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28240249/enhancing-the-anti-glioma-therapy-of-doxorubicin-by-honokiol-with-biodegradable-self-assembling-micelles-through-multiple-evaluations
#9
Xiang Gao, Ting Yu, Guangya Xu, Gang Guo, Xiaoxiao Liu, Xin Hu, Xiang Wang, Yanhui Liu, Qing Mao, Chao You, Liangxue Zhou
Combination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28183660/anticancer-activity-of-biologically-synthesized-silver-and-gold-nanoparticles-on-mouse-myoblast-cancer-cells-and-their-toxicity-against-embryonic-zebrafish
#10
Rajan Ramachandran, Chandran Krishnaraj, Allur Subramaniyan Sivakumar, Palaniappan Prasannakumar, V K Abhay Kumar, Kwan Seob Shim, Chul-Gyu Song, Soon-Il Yun
The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C2C12). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV-Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28129614/effects-of-titanium-dioxide-nanoparticles-exposure-on-parkinsonism-in-zebrafish-larvae-and-pc12
#11
Qinglian Hu, Fengliang Guo, Fenghui Zhao, Zhengwei Fu
Nanomaterials hold significant potential for industrial and biomedical application these years. Therefore, the relationship between nanoparticles and neurodegenerative disease is of enormous interest. In this contribution, zebrafish embryos and PC12 cell lines were selected for studying neurotoxicity of titanium dioxide nanoparticles (TiO2 NPs). After exposure of different concentrations of TiO2 NPs to embryos from fertilization to 96 hpf, the hatching time of zebrafish was decreased, accompanied by an increase in malformation rate...
January 16, 2017: Chemosphere
https://www.readbyqxmd.com/read/28127503/embryonic-zebrafish-model-a-well-established-method-for-rapidly-assessing-the-toxicity-of-homeopathic-drugs-toxicity-evaluation-of-homeopathic-drugs-using-zebrafish-embryo-model
#12
Himanshu R Gupta, Yogesh Patil, Dipty Singh, Mansee Thakur
OBJECTIVES: Advancements in nanotechnology have led to nanoparticle (NP) use in various fields of medicine. Although the potential of NPs is promising, the lack of documented evidence on the toxicological effects of NPs is concerning. A few studies have documented that homeopathy uses NPs. Unfortunately, very few sound scientific studies have explored the toxic effects of homeopathic drugs. Citing this lack of high-quality scientific evidence, regulatory agencies have been reluctant to endorse homeopathic treatment as an alternative or adjunct treatment...
December 2016: Journal of Pharmacopuncture
https://www.readbyqxmd.com/read/28124705/responsive-mesoporous-silica-nanoparticles-for-sensing-of-hydrogen-peroxide-and-simultaneous-treatment-toward-heart-failure
#13
Si Yu Tan, Cathleen Teh, Chung Yen Ang, Menghuan Li, Peizhou Li, Vladimir Korzh, Yanli Zhao
Chronic heart failure is often characterized by the elevated amounts of reactive oxygen species such as hydrogen peroxide (H2O2) in the heart. Thus, it is of importance that selective release of therapeutic drugs occurs at the heart failure site to maximize therapeutic effects. In this work, functional mesoporous silica nanoparticles (MSNPs) were developed for detection of H2O2, selective drug release and controlled treatment toward heart failure. The H2O2-sensitive probe was attached to the surface of the MSNPs, and a therapeutic drug of heart failure, captopril, was loaded within the pores of the MSNPs and retained by the binding of α-cyclodextrin to the probe...
January 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28104549/mechanisms-of-silver_nanoparticles-induced-hypopigmentation-in-embryonic-zebrafish
#14
Lian Xu, Qin-Han Xu, Xin-Ying Zhou, Li-Yan Yin, Peng-Peng Guan, Ting Zhang, Jing-Xia Liu
Silver_nanoparticles (AgNPs) have been reported to inhibit specification of erythroid cells and to induce spinal cord deformities and cardiac arrhythmia in vertebrates, but have not been implicated in development of neural crest (NC) and pigment cells in an in vivo model yet. In current study, down-regulated expressions of NC genes pax7 and foxd3, melanophore genes mitfa and dct, and xanthophore gene gch2 in AgNPs-exposed embryos were revealed by microarray, qRT-PCR and whole-mount in situ hybridization (WISH)...
March 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28061455/enhancement-of-the-bioavailability-of-a-novel-anticancer-compound-acetyltanshinone-iia-by-encapsulation-within-mpeg-plga-nanoparticles-a-study-of-formulation-optimization-toxicity-and-pharmacokinetics
#15
Qi Wang, Na Wei, Xiaofeng Liu, Alex Chang, Kathy Qian Luo
The Poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) (mPEG-PLGA) nanoparticles carrying acetyltanshinone IIA (ATA), a novel anti-breast cancer agent, were prepared by ultrasonic emulsion method to enhance the bioavailability and reduce the toxicity. Systematic optimization of encapsulation process was achieved using an orthogonal design. Drug efficacy analysis showed that ATA nanoparticles were as effective as free ATA against estrogen receptor positive breast cancer cells, but much less toxic towards human endothelial cells...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28026936/safe-by-design-cuo-nanoparticles-via-fe-doping-cu-o-bond-length-variation-and-biological-assessment-in-cells-and-zebrafish-embryos
#16
Hendrik Naatz, Sijie Lin, Ruibin Li, Wen Jiang, Zhaoxia Ji, Chong Hyun Chang, Jan Köser, Jorg Thöming, Tian Xia, Andre E Nel, Lutz Mädler, Suman Pokhrel
The safe implementation of nanotechnology requires nanomaterial hazard assessment in accordance with the material physicochemical properties that trigger the injury response at the nano/bio interface. Since CuO nanoparticles (NPs) are widely used industrially and their dissolution properties play a major role in hazard potential, we hypothesized that tighter bonding of Cu to Fe by particle doping could constitute a safer-by-design approach through decreased dissolution. Accordingly, we designed a combinatorial library in which CuO was doped with 1-10% Fe in a flame spray pyrolysis reactor...
January 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28009508/synthesis-and-antibacterial-study-of-sulfobetaine-quaternary-ammonium-modified-star-shaped-poly-2-dimethylamino-ethyl-methacrylate-based-copolymers-with-an-inorganic-core
#17
Yuji Pu, Zheng Hou, Mya Mya Khin, Rubi Zamudio-Vázquez, Kar Lai Poon, Hongwei Duan, Mary B Chan-Park
Cationic polymethacrylates are interesting candidates for bacterial disinfectants since they can be made in large-scale by various well-established polymerization techniques such as atom transfer radical polymerization (ATRP). However, they are usually toxic or ineffective in serum and various strategies to improve their biocompatibility or nonfouling property have often resulted in compromised bactericidal activity. Also, star-shaped polymers are less explored than linear polymers for application as antibacterial compounds...
December 23, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/28008795/a-comparison-of-fate-and-toxicity-of-selenite-biogenically-and-chemically-synthesized-selenium-nanoparticles-to-zebrafish-danio-rerio-embryogenesis
#18
Joyabrata Mal, Wouter J Veneman, Y V Nancharaiah, Eric D van Hullebusch, Willie J G M Peijnenburg, Martina G Vijver, Piet N L Lens
Microbial reduction of selenium (Se) oxyanions to elemental Se is a promising technology for bioremediation and treatment of Se wastewaters. But a fraction of biogenic nano-Selenium (nano-Se(b)) formed in bioreactors remains suspended in the treated waters, thus entering the aquatic environment. The present study investigated the toxicity of nano-Se(b) formed by anaerobic granular sludge biofilms on zebrafish embryos in comparison with selenite and chemogenic nano-Se (nano-Se(c)). The nano-Se(b) formed by granular sludge biofilms showed a LC50 value of 1...
January 9, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/27999267/zebrafish-as-a-vertebrate-model-system-to-evaluate-effects-of-environmental-toxicants-on-cardiac-development-and-function
#19
REVIEW
Swapnalee Sarmah, James A Marrs
Environmental pollution is a serious problem of the modern world that possesses a major threat to public health. Exposure to environmental pollutants during embryonic development is particularly risky. Although many pollutants have been verified as potential toxicants, there are new chemicals in the environment that need assessment. Heart development is an extremely sensitive process, which can be affected by environmentally toxic molecule exposure during embryonic development. Congenital heart defects are the most common life-threatening global health problems, and the etiology is mostly unknown...
December 16, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27995293/comparative-analysis-of-transcriptional-profile-changes-in-larval-zebrafish-exposed-to-zinc-oxide-nanoparticles-and-zinc-sulfate
#20
Ryeo-Ok Kim, Jin Soo Choi, Byoung-Chul Kim, Woo-Keun Kim
Many studies of the toxic effects of zinc oxide nanoparticles (ZnO NPs) in aquatic organisms have been performed because of increasing ZnO NP use. However, the toxicological pathways are not understood. In this study, ZnO NPs were found to be more toxic than ZnSO4 to zebrafish larvae, but ZnO NP toxicity did not involve transcript alterations. Biological processes affected by ZnO NPs and ZnSO4 were investigated by performing ingenuity pathway analysis on differently expressed genes in larvae exposed to sub-lethal ZnO NP and ZnSO4 concentrations...
February 2017: Bulletin of Environmental Contamination and Toxicology
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