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biofilm producing E. coli

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https://www.readbyqxmd.com/read/28081347/virulence-factors-biofilm-forming-ability-and-antimicrobial-resistance-of-urinary-escherichia-coli-strains-isolated-from-hospitalized-patients
#1
Barbara Kot, Jolanta Wicha, Agata Grużewska, Małgorzata Piechota, Katarzyna Wolska, Maria Obrębska
BACKGROUND/AIM: Escherichia coli is the most frequent cause of urinary tract infections. We investigated the possible associations between the origin of strains, antimicrobial resistance, the presence of urovirulence factors, and biofilm-forming ability. MATERIALS AND METHODS: Antibiotic susceptibility of E. coli strains was tested by disk diffusion method. Hemagglutination assays were performed for phenotypic characterization of the cell surface. Multiplex PCR was used for detection of virulence genes and for determination of phylogenetic relationships...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28073397/identification-of-new-members-of-the-escherichia-coli-k-12-mg1655-slya-regulon
#2
Thomas Curran, Fatima Abacha, Stephen Hibberd, Matthew Rolfe, Melissa Lacey, Jeffrey Green
SlyA is a member of the MarR family of bacterial transcriptional regulators. Previously, SlyA has been shown to directly regulate only two operons in Escherichia coli K-12 MG1655, fimB and hlyE (clyA). In both cases SlyA activates gene expression by antagonizing repression by the nucleoid associated protein H-NS. Here the transcript profiles of aerobic glucose-limited steady-state chemostat cultures of E. coli K-12 MG1655, slyA mutant and slyA over-expression strains are reported. The transcript profile of the slyA mutant was not significantly different to that of the parent; however, that of the slyA expression strain was significantly different from that of the vector control...
January 10, 2017: Microbiology
https://www.readbyqxmd.com/read/28039788/evaluation-of-the-catalytic-antibacterial-and-anti-biofilm-activities-of-the-convolvulus-arvensis-extract-functionalized-silver-nanoparticles
#3
Sepideh Hamedi, Seyed Abbas Shojaosadati, Aref Mohammadi
In this study, a rapid green synthesis of the silver nanoparticles (AgNPs) using leaves extract of Convolvulus arvensis was investigated. UV-Visible spectra showed the SPR band at around 430nm which proved the AgNP formation. The reduction of silver ions to nanoparticles was completed within 150min. Particle size analysis verified the formation of AgNPs with average size of 28nm, while their PDI was 0.2. XRD patterns verified the crystalline nature of produced AgNPs. SEM images verified the presence of spherical nanoparticles with no evidence of aggregations...
December 21, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28011696/genotypic-characterization-and-biofilm-formation-of-shiga-toxin-producing-escherichia-coli
#4
C Picozzi, D Antoniani, I Vigentini, R Foschino
Shiga toxin producing Escherichia coli (STEC) are recognized as one of the most dangerous food-borne pathogens. The production of Shiga toxins together with intimin protein are among the main virulence factors. However, the ability to form biofilm can protect bacteria against environmental factors (i.e. desiccation, exposure to UV rays, predation, etc) and sanitization procedures (cleaning, rinsing, chlorination), increasing their survival on food products and in manufacturing plants. Forty-five isolates collected from food and fecal samples were genotyped by Pulsed Field Gel Electrophoresis (PFGE) analysis with XbaI restriction enzyme and investigated by searching for toxins (stx1, stx2) and intimin (eae) genes and serogroup (O157, O26, O145, O111, O103 and O104)...
December 22, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27889162/tolerance-of-clostridium-perfringens-biofilms-to-disinfectants-commonly-used-in-the-food-industry
#5
Audrey Charlebois, Mario Jacques, Martine Boulianne, Marie Archambault
Clostridium perfringens is an opportunistic pathogen that can cause food poisoning in humans and various enterotoxemia in animal species. Recently, it was shown to form mono-species biofilms, a structured community of bacterial cells enclosed in a self-produced extracellular matrix. Biofilms have been associated with tolerance to antibiotics, disinfectants, and physical and environmental stresses. Very little is known about the tolerance of C. perfringens biofilm toward disinfectants. In the present study, susceptibilities of C...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27884783/secondary-multidrug-efflux-pump-mutants-alter-escherichia-coli-biofilm-growth-in-the-presence-of-cationic-antimicrobial-compounds
#6
Denice C Bay, Carol A Stremick, Carmine J Slipski, Raymond J Turner
Escherichia coli possesses many secondary active multidrug resistance transporters (MDTs) that confer overlapping substrate resistance to a broad range of antimicrobials via proton and/or sodium motive force. It is uncertain whether redundant MDTs uniquely alter cell survival when cultures grow planktonically or as biofilms. In this study, the planktonic and biofilm growth and antimicrobial resistance of 13 E. coli K-12 single MDT gene deletion strains in minimal and rich media were determined. Antimicrobial tolerance to tetracycline, tobramycin and benzalkonium were also compared for each ΔMDT strain...
November 22, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27878718/proinflammatory-cytokines-as-regulators-of-vaginal-microbiota
#7
E A Kremleva, A V Sgibnev
It was shown that IL-1β, IL-8, and IL-6 in concentrations similar to those in the vagina of healthy women stimulated the growth of normal microflora (Lactobacillus spp.) and suppressed the growth and biofilm production by S. aureus and E. coli. On the contrary, these cytokines in higher concentrations typical of vaginal dysbiosis suppressed normal microflora and stimulated the growth of opportunistic microorganisms. TGF-β1 in both doses produced a stimulating effects on study vaginal microsymbionts. It is hypothesized that pro-inflammatory cytokines serve as the molecules of interspecies communication coordinating the interactions of all components of the vaginal symbiotic system...
November 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27812526/evaluation-and-selection-of-bacillus-species-based-on-enzyme-production-antimicrobial-activity-and-biofilm-synthesis-as-direct-fed-microbial-candidates-for-poultry
#8
Juan D Latorre, Xochitl Hernandez-Velasco, Ross E Wolfenden, Jose L Vicente, Amanda D Wolfenden, Anita Menconi, Lisa R Bielke, Billy M Hargis, Guillermo Tellez
Social concern about misuse of antibiotics as growth promoters (AGP) and generation of multidrug-resistant bacteria have restricted the dietary inclusion of antibiotics in livestock feed in several countries. Direct-fed microbials (DFM) are one of the multiple alternatives commonly evaluated as substitutes of AGP. Sporeformer bacteria from the genus Bacillus have been extensively investigated because of their extraordinary properties to form highly resistant endospores, produce antimicrobial compounds, and synthesize different exogenous enzymes...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/27762637/dextranase-from-arthrobacter-oxydans-kq11-1-inhibits-biofilm-formation-by-polysaccharide-hydrolysis
#9
Xiaobei Wang, Huaixu Cheng, Mingsheng Lu, Yaowei Fang, Yuliang Jiao, Weijuan Li, Gengmao Zhao, Shujun Wang
Dental plaque is a biofilm of water-soluble and water-insoluble polysaccharides, produced primarily by Streptococcus mutans. Dextranase can inhibit biofilm formation. Here, a dextranase gene from the marine microorganism Arthrobacter oxydans KQ11-1 is described, and cloned and expressed using E. coli DH5α competent cells. The recombinant enzyme was then purified and its properties were characterized. The optimal temperature and pH were determined to be 60°C and 6.5, respectively. High-performance liquid chromatography data show that the final hydrolysis products were glucose, maltose, maltotriose, and maltotetraose...
November 2016: Biofouling
https://www.readbyqxmd.com/read/27744103/detection-of-ampc-%C3%AE-lactamase-and-adherence-factors-in-uropathogenic-escherichia-coli-isolated-from-aged-patients
#10
Santosh Kumar Singh, Kumari Seema, Minakshi Gupta
Escherichia coli mediated urinary tract infection has been reported to be most prevalent among patients of different class, gender and ages. Currently, multidrug resistant E. coli harboring several virulence factors are most perilous threats for patients especially for elders. The aim of this study was to determine the antibiotic resistance pattern, co-resistance and phenotypic virulence factors present in uropathogenic E. coli isolated from aged patients. Thirty-nine E. coli isolates were collected during May-June 2014 from patients between 50 to 80 years of age...
November 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27698083/the-catabolite-repressor-protein-cyclic-amp-complex-regulates-csgd-and-biofilm-formation-in-uropathogenic-escherichia-coli
#11
David A Hufnagel, Margery L Evans, Sarah E Greene, Jerome S Pinkner, Scott J Hultgren, Matthew R Chapman
The extracellular matrix protects Escherichia coli from immune cells, oxidative stress, predation, and other environmental stresses. Production of the E. coli extracellular matrix is regulated by transcription factors that are tuned to environmental conditions. The biofilm master regulator protein CsgD upregulates curli and cellulose, the two major polymers in the extracellular matrix of uropathogenic E. coli (UPEC) biofilms. We found that cyclic AMP (cAMP) regulates curli, cellulose, and UPEC biofilms through csgD The alarmone cAMP is produced by adenylate cyclase (CyaA), and deletion of cyaA resulted in reduced extracellular matrix production and biofilm formation...
December 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27687245/chemotaxis-towards-autoinducer-2-mediates-autoaggregation-in-escherichia-coli
#12
Leanid Laganenka, Remy Colin, Victor Sourjik
Bacteria communicate by producing and sensing extracellular signal molecules called autoinducers. Such intercellular signalling, known as quorum sensing, allows bacteria to coordinate and synchronize behavioural responses at high cell densities. Autoinducer 2 (AI-2) is the only known quorum-sensing molecule produced by Escherichia coli but its physiological role remains elusive, although it is known to regulate biofilm formation and virulence in other bacterial species. Here we show that chemotaxis towards self-produced AI-2 can mediate collective behaviour-autoaggregation-of E...
September 30, 2016: Nature Communications
https://www.readbyqxmd.com/read/27645299/acetate-metabolism-regulation-in-escherichia-coli-carbon-overflow-pathogenicity-and-beyond
#13
REVIEW
Vicente Bernal, Sara Castaño-Cerezo, Manuel Cánovas
Acetate is ubiquitously found in natural environments. Its availability in the gut is high as a result of the fermentation of nutrients, and although it is rapidly absorbed by intestinal mucosa, it can also be used as carbon source by some members of gut microbiota. The metabolism of acetate in Escherichia coli has attracted the attention of the scientific community due to its role in central metabolism and its link to multiple physiological features. In this microorganism, acetate is involved directly or indirectly on the regulation of functional processes, such as motility, formation of biofilms, and responses to stress...
November 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27638396/indole-production-provides-limited-benefit-to-escherichia-coli-during-co-culture-with-enterococcus-faecalis
#14
Shelly L Pringle, Kelli L Palmer, Robert J C McLean
Escherichia coli lives in the gastrointestinal tract and elsewhere, where it coexists within a mixed population. Indole production enables E. coli to grow with other gram-negative bacteria as indole inhibits N-acyl-homoserine lactone (AHL) quorum regulation. We investigated whether E. coli indole production enhanced competition with gram-positive Enterococcus faecalis, wherein quorum signaling is mediated by small peptides. During planktonic co-culture with E. faecalis, the fitness and population density of E...
September 16, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27626397/flavones-as-quorum-sensing-inhibitors-identified-by-a-newly-optimized-screening-platform-using-chromobacterium-violaceum-as-reporter-bacteria
#15
Malena E Skogman, Sonja Kanerva, Suvi Manner, Pia M Vuorela, Adyary Fallarero
Quorum sensing (QS) is the process by which bacteria produce and detect signal molecules to coordinate their collective behavior. This intercellular communication is a relevant target for anti-biofilm therapies. Here we have optimized a screening-applicable assay to search for new quorum sensing inhibitors from natural compound libraries. In this system, QS is correlated with the production of violacein, which is directly controlled by the LuxI/LuxR system in Chromobacterium violaceum ATCC 31532. The parallel use of C...
September 10, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27623312/cell-based-high-throughput-screening-identifies-rifapentine-as-an-inhibitor-of-amyloid-and-biofilm-formation-in-escherichia-coli
#16
Marie C Maher, Ji Youn Lim, Cheston Gunawan, Lynette Cegelski
Escherichia coli assemble functional amyloid fibers termed curli that contribute to bacterial adhesion, biofilm formation, and host pathogenesis. We developed a cell-based high-throughput screen to identify inhibitors of curli-mediated adhesion in the laboratory strain MC4100 and curli-associated biofilm formation in the uropathogenic E. coli clinical isolate UTI89. Inhibitors of biofilm formation can operate through many mechanisms, and such inhibitors could hold therapeutic value in preventing and treating urinary tract infections...
October 9, 2015: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27524089/plasma-polymerized-carvone-as-an-antibacterial-and-biocompatible-coating
#17
Yuen Wah Chan, Kim Shyong Siow, Pei Yuen Ng, Usup Gires, Burhanuddin Yeop Majlis
Antibacterial coating is important to prevent the colonization of medical devices by biofilm forming bacteria that would cause infection and sepsis in patients. Current coating techniques such as immobilization of antimicrobial compounds, time-releasing antibiotic agents and silver nanoparticles, require multiple processing steps, and they have low efficacy and low stability. We proposed a single-step plasma polymerization of an essential oil known as carvone to produce a moderately hydrophobic antibacterial coating (ppCar) with an average roughness of <1nm...
November 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27497060/macroscopic-amyloid-fiber-formation-by-staphylococcal-biofilm-associated-suhb-protein
#18
Anirudha Dutta, Sudipta Bhattacharyya, Anirban Kundu, Debabrata Dutta, Amit Kumar Das
Staphylococcus aureus is a commensal and opportunistic pathogen that causes lethal infections. Biofilm forming ability of S. aureus enhances its virulence since biofilm provides the bacteria protective shield against antibiotics and host immunity. Polysaccharide independent biofilm formation by several virulent S. aureus strains have been identified recently, where protein components substitute polysaccharide intercellular adhesin (PIA) involved in bacterial cell attachment. The suhB gene has been reported to be essential in staphylococcal PIA-independent biofilm formation...
October 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27252465/phylogenetic-analysis-of-enteroaggregative-escherichia-coli-eaec-isolates-from-japan-reveals-emergence-of-ctx-m-14-producing-eaec-o25-h4-clones-related-to-sequence-type-131
#19
Naoko Imuta, Tadasuke Ooka, Kazuko Seto, Ryuji Kawahara, Toyoyasu Koriyama, Tsuyoshi Kojyo, Atsushi Iguchi, Koichi Tokuda, Hideki Kawamura, Kiyotaka Yoshiie, Yoshitoshi Ogura, Tetsuya Hayashi, Junichiro Nishi
Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum β-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the blaCTX-M gene, and the capacity to form biofilms...
August 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27248296/a-rich-morphological-diversity-of-biosaline-drying-patterns-is-generated-by-different-bacterial-species-different-salts-and-concentrations-astrobiological-implications
#20
José María Gómez Gómez, Jesús Medina, Fernando Rull
UNLABELLED: Biosaline formations (BSFs) are complex self-organized biomineral patterns formed by "hibernating" bacteria as the biofilm that contains them dries out. They were initially described in drying biofilms of Escherichia coli cells + NaCl. Due to their intricate 3-D morphology and anhydrobiosis, these biomineralogical structures are of great interest in astrobiology. Here we report experimental data obtained with various alkali halide salts (NaF, NaCl, NaBr, LiCl, KCl, CsCl) on BSF formation with E...
July 2016: Astrobiology
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