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biofilm producing E. coli

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https://www.readbyqxmd.com/read/29762495/role-of-sdia-on-biofilm-formation-by-atypical-enteropathogenic-escherichia-coli
#1
Hebert F Culler, Samuel C F Couto, Juliana S Higa, Renato M Ruiz, Min J Yang, Vanessa Bueris, Marcia R Franzolin, Marcelo P Sircili
Atypical enteropathogenic Escherichia coli are capable to form biofilm on biotic and abiotic surfaces, regardless of the adherence pattern displayed. Several E. coli mechanisms are regulated by Quorum sensing (QS), including virulence factors and biofilm formation. Quorum sensing is a signaling system that confers bacteria with the ability to respond to chemical molecules known as autoinducers. Suppressor of division inhibitor (SdiA) is a QS receptor present in atypical enteropathogenic E. coli (aEPEC) that detects acyl homoserine lactone (AHL) type autoinducers...
May 15, 2018: Genes
https://www.readbyqxmd.com/read/29760866/short-genome-report-of-cellulose-producing-commensal-escherichia-coli-1094
#2
Joaquin Bernal-Bayard, Laura Gomez-Valero, Aimee Wessel, Varun Khanna, Christiane Bouchier, Jean-Marc Ghigo
Bacterial surface colonization and biofilm formation often rely on the production of an extracellular polymeric matrix that mediates cell-cell and cell-surface contacts. In Escherichia coli and many Betaproteobacteria and Gammaproteobacteria cellulose is often the main component of the extracellular matrix. Here we report the complete genome sequence of the cellulose producing strain E. coli 1094 and compare it with five other closely related genomes within E. coli phylogenetic group A. We present a comparative analysis of the regions encoding genes responsible for cellulose biosynthesis and discuss the changes that could have led to the loss of this important adaptive advantage in several E...
2018: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29758033/phylogenetic-relationships-biofilm-formation-motility-antibiotic-resistance-and-extended-virulence-genotypes-among-escherichia-coli-strains-from-women-with-community-onset-primitive-acute-pyelonephritis
#3
Arianna Pompilio, Valentina Crocetta, Vincenzo Savini, Dezemona Petrelli, Marta Di Nicola, Silvia Bucco, Luigi Amoroso, Mario Bonomini, Giovanni Di Bonaventura
The present work set out to search for a virulence repertoire distinctive for Escherichia coli causing primitive acute pyelonephritis (APN). To this end, the virulence potential of 18 E. coli APN strains was genotypically and phenotypically assessed, comparatively with 19 strains causing recurrent cystitis (RC), and 16 clinically not significant (control, CO) strains. Most of the strains belong to phylogenetic group B1 (69.8%; p<0.01), and APN strains showed unique features, which are the presence of phylogroup A, and the absence of phylogroup B2 and non-typeable strains...
2018: PloS One
https://www.readbyqxmd.com/read/29739319/characterization-of-biofilm-forming-capacity-and-resistance-to-sanitizers-of-a-range-of-e-coli-o26-pathotypes-from-clinical-cases-and-cattle-in-australia
#4
Salma A Lajhar, Jeremy Brownlie, Robert Barlow
BACKGROUND: The formation of biofilms and subsequent encasement of bacterial cells in a complex matrix can enhance resistance to antimicrobials and sterilizing agents making these organisms difficult to eradicate and control. The aim of this study was to evaluate and compare the capacity of 40 E. coli O26 isolates of enterohemorrhagic E. coli (EHEC, n = 27), potential EHEC (pEHEC, n = 3), atypical enteropathogenic E. coli (aEPEC, n = 8) and non-toxigenic E. coli (NTEC, n = 2) from human and cattle sources to form biofilms on different surfaces, and determine whether extracellular matrix (ECM) components (cellulose, curli), motility, prophage insertion in mlrA and cell surface hydrophobicity could influence biofilm formation...
May 8, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29690761/printing-of-patterned-engineered-e-coli-biofilms-with-a-low-cost-3d-printer
#5
Dominik Tobias Schmieden, Samantha J Basalo Vázquez, Hector Sangüesa, Marit van der Does, Timon Idema, Anne Sara Meyer
Biofilms can grow on virtually any surface available, with impacts ranging from endangering the lives of patients to degrading unwanted water contaminants. Biofilm research is challenging due to the high degree of biofilm heterogeneity. A method for the production of standardized, reproducible, and patterned biofilm-inspired materials could be a boon for biofilm research and allow for completely new engineering applications. Here, we present such a method, combining 3D printing with genetic engineering. We prototyped a low-cost 3D printer that prints bioink, a suspension of bacteria in a solution of alginate that solidifies on a calcium-containing substrate...
April 24, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29684093/pgab-orthologues-contain-a-glycoside-hydrolase-domain-that-cleaves-deacetylated-poly-%C3%AE-1-6-n-acetylglucosamine-and-can-disrupt-bacterial-biofilms
#6
Dustin J Little, Roland Pfoh, François Le Mauff, Natalie C Bamford, Christina Notte, Perrin Baker, Manita Guragain, Howard Robinson, Gerald B Pier, Mark Nitz, Rajendar Deora, Donald C Sheppard, P Lynne Howell
Poly-β(1,6)-N-acetyl-D-glucosamine (PNAG) is a major biofilm component of many pathogenic bacteria. The production, modification, and export of PNAG in Escherichia coli and Bordetella species require the protein products encoded by the pgaABCD operon. PgaB is a two-domain periplasmic protein that contains an N-terminal deacetylase domain and a C-terminal PNAG binding domain that is critical for export. However, the exact function of the PgaB C-terminal domain remains unclear. Herein, we show that the C-terminal domains of Bordetella bronchiseptica PgaB (PgaBBb) and E...
April 23, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29657925/biofilm-formation-in-nonmultidrug-resistant-escherichia-coli-isolated-from-patients-with-urinary-tract-infection-in-isfahan-iran
#7
Farkhondeh Poursina, Shima Sepehrpour, Sina Mobasherizadeh
Background: Escherichia coli is a Gram-negative, opportunistic human pathogen in which increasing antibiotic resistance is a great concern for continued human survival. Although biofilm formation is a mechanism that helps E. coli to survive in unfavorable conditions, according to the importance of biofilm formation in developing the antibiotic resistance here, we studied the relation between antibiotic resistance and in vitro qualitative rating method biofilm formation in E. coli isolated from patients with urinary tract infection (UTI)...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29643424/poultry-hatcheries-as-potential-reservoirs-for-antimicrobial-resistant-escherichia-coli-a-risk-to-public-health-and-food-safety
#8
Kamelia M Osman, Anthony D Kappell, Mohamed Elhadidy, Fatma ElMougy, Wafaa A Abd El-Ghany, Ahmed Orabi, Aymen S Mubarak, Turki M Dawoud, Hassan A Hemeg, Ihab M I Moussa, Ashgan M Hessain, Hend M Y Yousef
Hatcheries have the power to spread antimicrobial resistant (AMR) pathogens through the poultry value chain because of their central position in the poultry production chain. Currently, no information is available about the presence of AMR Escherichia coli strains and the antibiotic resistance genes (ARGs) they harbor within hatchezries. Therefore, this study aimed to investigate the possible involvement of hatcheries in harboring hemolytic AMR E. coli. Serotyping of the 65 isolated hemolytic E. coli revealed 15 serotypes with the ability to produce moderate biofilms, and shared susceptibility to cephradine and fosfomycin and resistance to spectinomycin...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29625657/multicellular-behavior-of-environmental-escherichia-coli-isolates-grown-under-nutrient-poor-and-low-temperature-conditions
#9
Laura Di Sante, Armanda Pugnaloni, Francesca Biavasco, Eleonora Giovanetti, Carla Vignaroli
The multicellular behavior designated "red dry and rough" (rdar) morphotype-characterized by production of extracellular matrix mainly comprising curli fimbriae and cellulose-is a potential survival strategy of Escherichia coli outside the host. This study documents the ability of Escherichia cryptic clades, which have recently been recognized as new lineages genetically divergent from E. coli, to grow in unfavorable conditions through expression of distinct phenotypes. Growth under low-temperature and nutrient-poor conditions induced the rdar morphotype in all cryptic clade strains tested, especially after preincubation in broth supplemented with uracil...
May 2018: Microbiological Research
https://www.readbyqxmd.com/read/29593666/factors-involved-in-the-persistence-of-a-shiga-toxin-producing-escherichia-coli-o157-h7-strain-in-bovine-feces-and-gastro-intestinal-content
#10
Audrey Segura, Pauline Auffret, Delphine Bibbal, Marine Bertoni, Alexandra Durand, Grégory Jubelin, Monique Kérourédan, Hubert Brugère, Yolande Bertin, Evelyne Forano
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E. coli responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coli O157:H7 outside the bovine gut. The E. coli O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29592871/cystathionine-%C3%AE-lyase-is-involved-in-d-amino-acid-metabolism
#11
Tetsuya Miyamoto, Masumi Katane, Yasuaki Saitoh, Masae Sekine, Hiroshi Homma
Non-canonical D-amino acids play important roles in bacteria including control of peptidoglycan metabolism and biofilm disassembly. Bacteria appear to produce non-canonical D-amino acids to adapt to various environmental changes, and understanding the biosynthetic pathways is important. We identified novel amino acid racemases possessing the ability to produce non-canonical D-amino acids in Escherichia coli and Bacillus subtilis in our previous study, whereas the biosynthetic pathways of these D-amino acids still remain unclear...
March 28, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29581075/distribution-of-extended-spectrum-%C3%AE-lactam-quinolone-and-carbapenem-resistance-genes-and-genetic-diversity-among-uropathogenic-escherichia-coli-isolates-in-tehran-iran
#12
Shahla Shahbazi, Mohammad Reza Asadi Karam, Mehri Habibi, Atefeh Talebi, Saeid Bouzari
OBJECTIVES: Antibiotic resistance among the Uropathogenic Escherichia coli (UPEC) is increasing. The aim of this study was to evaluate antimicrobial resistance in the UPEC isolates and their correlations with virulence factors. METHODS: A total of 120 E. coli isolates were collected from the patients with urinary tract infections in Tehran, Iran. Biofilm formation and antimicrobial susceptibilities were determined by phenotypic tests. Combination disk diffusion and modified Hodge tests (MHT) were performed for the phenotypic detection of ESBL and carbapenemase, respectively...
March 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29581066/no-lower-bacterial-adhesion-for-ceramics-compared-to-other-biomaterials-an-in-vitro-analysis
#13
P A Slullitel, M A Buttaro, G Greco, J I Oñativia, M L Sánchez, S Mc Loughlin, C García-Ávila, F Comba, G Zanotti, F Piccaluga
BACKGROUND: Although there is some clinical evidence of ceramic bearings being associated with a lower infection rate after total hip arthroplasty (THA), available data remains controversial since this surface is usually reserved for young, healthy patients. Therefore, we investigated the influence of five commonly-used biomaterials on the adhesion potential of four biofilm-producing bacteria usually detected in infected THAs. HYPOTHESIS: Ceramic biomaterials exhibit less bacterial adherence than other biomaterials...
March 23, 2018: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/29577084/aggregative-adherence-and-intestinal-colonization-by-enteroaggregative-escherichia-coli-are-produced-by-interactions-among-multiple-surface-factors
#14
Laura V Blanton, Lawrence T Wang, Jennifer Hofmann, Joshua DuBow, Alexander Lafrance, Stephen Kwak, Levi Bowers, Mandara A Levine, Charles O Hale, Philip M Meneely, Iruka N Okeke
Enteroaggregative Escherichia coli (EAEC) bacteria are exceptional colonizers that are associated with diarrhea. The genome of EAEC strain 042, a diarrheal pathogen validated in a human challenge study, encodes multiple colonization factors. Notable among them are aggregative adherence fimbriae (AAF/II) and a secreted antiaggregation protein (Aap). Deletion of aap is known to increase adherence, autoaggregation, and biofilm formation, so it was proposed that Aap counteracts AAF/II-mediated interactions. We hypothesized that Aap sterically masks heat-resistant agglutinin 1 (Hra1), an integral outer membrane protein recently identified as an accessory colonization factor...
March 2018: MSphere
https://www.readbyqxmd.com/read/29571086/monitoring-biofilm-function-in-new-and-matured-full-scale-slow-sand-filters-using-flow-cytometric-histogram-image-comparison-chic
#15
Sandy Chan, Kristjan Pullerits, Janine Riechelmann, Kenneth M Persson, Peter Rådström, Catherine J Paul
While slow sand filters (SSFs) have produced drinking water for more than a hundred years, understanding of their associated microbial communities is limited. In this study, bacteria in influent and effluent water from full-scale SSFs were explored using flow cytometry (FCM) with cytometric histogram image comparison (CHIC) analysis; and routine microbial counts for heterotrophs, total coliforms and Escherichia coli. To assess if FCM can monitor biofilm function, SSFs differing in age and sand composition were compared...
March 13, 2018: Water Research
https://www.readbyqxmd.com/read/29543544/abundant-production-of-exopolysaccharide-by-eaec-strains-enhances-the-formation-of-bacterial-biofilms-in-contaminated-sprouts
#16
Quintin Borgersen, David T Bolick, Glynis L Kolling, Matthew Aijuka, Fernando Ruiz-Perez, Richard L Guerrant, James P Nataro, Araceli E Santiago
Enteroaggregative E. coli (EAEC) is associated with food-borne outbreaks of diarrhea and growth faltering among children in developing countries. A Shiga toxin-producing EAEC strain of serotype O104:H4 strain caused one of the largest outbreaks of a food-borne infection in Europe in 2011. The outbreak was traced to contaminated fenugreek sprouts, yet the mechanisms whereby such persistent contamination of sprouts could have occurred are not clear. We found that under ambient conditions of temperature and in minimal media, pathogenic Shiga toxin-producing EAEC O104:H4 227-11 and non-Shiga toxin-producing 042 strains both produce high levels of exopolysaccharide structures (EPS) that are released to the external milieu...
March 15, 2018: Gut Microbes
https://www.readbyqxmd.com/read/29512849/phenotypic-and-virulence-traits-of-escherichia-coli-and-salmonella-strains-isolated-from-vegetables-and-fruits-from-india
#17
P Verma, V V Saharan, S Nimesh, A P Singh
AIMS: The present study was designed to assess the phenotypic traits and virulence determinants of vegetable-/fruit-origin Escherichia coli and Salmonella strains. METHODS AND RESULTS: A total of 520 fresh vegetables/fruits samples were analysed for the presence of E. coli, including Shiga toxin-producing E. coli (STEC), and Salmonella. The vegetable-/fruit-origin E. coli and Salmonella strains were further assessed for antimicrobial resistance, biofilm formation, extracellular matrix production and in vitro invasion/intracellular survivability assays...
March 7, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29499267/nano-magnesium-oxide-reinforced-polylactic-acid-biofilms-for-food-packaging-applications
#18
Chetan Swaroop, Mukul Shukla
This study is aimed at producing biofilms by reinforcement of Magnesium Oxide (MgO) nanoparticles in polylactic acid (PLA) biopolymer using the solvent casting method. In this study MgO nanoparticles (up to 4wt%) were reinforced in PLA biopolymer and their key mechanical, barrier, thermal and antibacterial properties were investigated for food packaging applications. Among the prepared biocomposite films, the 2wt% reinforced PLA films showed the maximum improvement in tensile strength and oxygen barrier properties (up to 29% and 25% respectively) in comparison to pristine PLA films...
July 1, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29472619/modulating-bacterial-and-gut-mucosal-interactions-with-engineered-biofilm-matrix-proteins
#19
Anna M Duraj-Thatte, Pichet Praveschotinunt, Trevor R Nash, Frederick R Ward, Neel S Joshi
Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are a class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use of engineered curli fibers to rationally program interactions between bacteria and components of the mucosal epithelium. Commensal E. coli strains were engineered to produce recombinant curli fibers fused to the trefoil family of human cytokines. Biofilms formed from these strains bound more mucins than those producing wild-type curli fibers, and modulated mucin rheology as well...
February 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29462463/typlex%C3%A2-chelate-a-novel-feed-additive-inhibits-campylobacter-jejuni-biofilm-formation-and-cecal-colonization-in-broiler-chickens
#20
F Khattak, V Paschalis, M Green, J G M Houdijk, P Soultanas, J Mahdavi
Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry, the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate (ferric tyrosine), a novel feed additive, in inhibiting Campylobacter jejuni (C...
April 1, 2018: Poultry Science
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