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biofilm producing E. coli

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https://www.readbyqxmd.com/read/29348344/draft-genome-sequence-of-bacillus-velezensis-osy-s3-a-producer-of-potent-antimicrobial-agents-active-against-bacteria-and-fungi
#1
Michelle M Gerst, Mustafa Yesil, Ahmed E Yousef
Bacillus velezensis OSY-S3 produces anti-Listeria, anti-Escherichia coli, and antifungal compounds. Additionally, fermentate of B. velezensis OSY-S3 culture removes Staphylococcus aureus biofilms effectively. The draft genome sequence of B. velezensis OSY-S3 reported here had a genome size of ~3.90 Mb and a G+C content of 46.5%.
January 18, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29345080/environmental-adaptation-and-vertical-dissemination-of-esbl-pampc-producing-escherichia-coli-in-an-integrated-broiler-production-chain-in-the-absence-of-an-antibiotic-treatment
#2
Michaela Projahn, Katrin Daehre, Torsten Semmler, Sebastian Guenther, Uwe Roesler, Anika Friese
High prevalence numbers of extended-spectrum beta-lactamase- (ESBL-)/plasmid-mediated AmpC beta-lactamase- (pAmpC-) producing Escherichia coli in broiler chicken and their distribution along the broiler production chain is an ongoing problem in food production. We, therefore, investigated resistant isolates along the broiler production chain to determine whether there is a constantly occurring direct vertical transmission of the ESBL-/pAmpC-producing E. coli from the parent flocks to their offspring or not...
January 17, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29335484/microbial-competition-of-rhodotorula-mucilaginosa-uanl-001l-and-e-coli-increase-biosynthesis-of-non-toxic-exopolysaccharide-with-applications-as-a-wide-spectrum-antimicrobial
#3
Augusto Vazquez-Rodriguez, Ximena G Vasto-Anzaldo, Daniel Barboza Perez, Eduardo Vázquez-Garza, Héctor Chapoy-Villanueva, Gerardo García-Rivas, Javier A Garza-Cervantes, Jéssica J Gómez-Lugo, Alma Elizabeth Gomez-Loredo, Maria Teresa Garza Gonzalez, Xristo Zarate, Jose Ruben Morones-Ramirez
Bacterial species are able to colonize and establish communities in biotic and abiotic surfaces. Moreover, within the past five decades, incidence of bacterial strains resistant to currently used antibiotics has increased dramatically. This has led to diverse health issues and economical losses for different industries. Therefore, there is a latent need to develop new and more efficient antimicrobials. This work reports an increased production of an exopolysaccharide in a native yeast strain isolated from the Mexican Northeast, Rhodotorula mucilaginosa UANL-001L, when co-cultured with E...
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29306732/sequential-effect-of-phages-and-cold-nitrogen-plasma-against-escherichia-coli-o157-h7-biofilms-on-different-vegetables
#4
Haiying Cui, Mei Bai, Lu Yuan, Duraiarasan Surendhiran, Lin Lin
Escherichia coli O157:H7 (E. coli O157:H7) is one of the most common pathogens in fresh vegetables and fruits, and most of the diseases produced by E. coli O157:H7 are associated with biofilms. Cold nitrogen plasma (CNP) is a cold sterilization technique which has no residue. However to completely eliminate the biofilm on the surface of vegetables the processing power and time of CNP have to be enhanced, which will impact on the quality of fruits and vegetables. Thus the sequential treatment of CNP and phage techniques was engineered in this study...
January 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29282051/microbiota-dysbiosis-in-inflammatory-bowel-diseases-in-silico-investigation-of-the-oxygen-hypothesis
#5
Michael A Henson, Poonam Phalak
BACKGROUND: Inflammatory bowel diseases (IBD), which include ulcerative colitis and Crohn's disease, cause chronic inflammation of the digestive tract in approximately 1.6 million Americans. A signature of IBD is dysbiosis of the gut microbiota marked by a significant reduction of obligate anaerobes and a sharp increase in facultative anaerobes. Numerous experimental studies have shown that IBD is strongly correlated with a decrease of Faecalibacterium prausnitzii and an increase of Escherichia coli...
December 28, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/29215297/virulence-genes-and-antimicrobial-resistance-in-escherichia-coli-from-cheese-made-from-unpasteurized-milk-in-brazil
#6
Anna C L P de Campos, Juan J Puño-Sarmiento, Leonardo P Medeiros, Luís E S Gazal, Renato Pariz Maluta, Armando Navarro, Renata K T Kobayashi, Eder P Fagan, Gerson Nakazato
Cow raw milk cheese is widely eaten in Brazil. These products may be contaminated with pathogenic bacteria. In this work, we investigated the presence of Escherichia coli in raw milk cheese from different States in Brazil. From 147 "Minas" cheese samples, 28 cheeses were positive for E. coli. Among 39 E. coli isolates of the cheeses, one was positive for eae and negative for bpfA and efa1/lifA using PCR, and so was classified as atypical Enteropathogenic E. coli (aEPEC). Two other isolates were positive for extraintestinal pathogenic E...
December 7, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29214000/role-of-antigen-43-on-biofilm-formation-and-horizontal-antibiotic-resistance-gene-transfer-in-non-o157-shiga-toxin-producing-escherichia-coli-strains
#7
Roholla Taghadosi, Mohammad Reza Shakibaie, Reza Ghanbarpour, Hossein Hosseini-Nave
Background and Objectives: The objectives of this study were to evaluate the antibiotic resistance profiles, biofilm formation, presence of antigen 43 (Ag43) gene, and transfer of antibiotic resistance phenotype among non-O157 Shiga toxin producing Escherichia coli (STEC). Materials and Methods: From October 2014 to November 2015 a total of 276 stool samples were collected from healthy calves, goats and 395 patients with the sign of nonbloody diarrhea and screened for presence of stx and serotype O157 genes by polymerase chain reaction (PCR) technique...
April 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#8
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29125461/chemotaxis-to-self-generated-ai-2-promotes-biofilm-formation-in-escherichia-coli
#9
Sneha Jani, Andrew L Seely, George L Peabody V, Arul Jayaraman, Michael D Manson
Responses to the interspecies quorum-sensing signal autoinducer-2 (AI-2) regulate the patterns of gene expression that promote biofilm development. Escherichia coli also senses AI-2 as a chemoattractant, a response that requires the periplasmic AI-2-binding protein LsrB and the chemoreceptor Tsr. Here, we confirm, as previously observed, that under static conditions highly motile E. coli cells self-aggregate and form surface-adherent structures more readily than cells lacking LsrB and Tsr, or than ΔluxS cells unable to produce AI-2...
November 9, 2017: Microbiology
https://www.readbyqxmd.com/read/29091715/antiquorum-sensing-antibiofilm-formation-and-cytotoxicity-activity-of-commonly-used-medicinal-plants-by-inhabitants-of-borabu-sub-county-nyamira-county-kenya
#10
Eric Omori Omwenga, Andreas Hensel, Susana Pereira, Alfred Anakalo Shitandi, Francisco M Goycoolea
Productions of various bacterial traits like production of virulence factors (e.g. toxins, enzymes), biofilm formation, luminescence among others, have been known to be controlled by quorum sensing (QS), a process that is dependent on chemical signals or autoinducers (AIs). Bacteria known to rely on such AIs are known to be virulent and tend to be resistant against various antimicrobial agents. Therefore, strategies aimed at the inhibition of QS pathways, are regarded as potential novel therapies in managing bacterial virulence hence reducing their ability to induce infections in humans...
2017: PloS One
https://www.readbyqxmd.com/read/29054868/conditional-function-of-autoaggregative-protein-cah-and-common-cah-mutations-in-shiga-toxin-producing-escherichia-coli
#11
Michelle Qiu Carter, Maria T Brandl, Indira T Kudva, Robab Katani, Matthew R Moreau, Vivek Kapur
Cah is a calcium-binding autotransporter protein involved in autoaggregation and biofilm formation. Although cah is widespread in Shiga toxin-producing Escherichia coli (STEC), we detected mutations in cah at a frequency of 31.3% in this pathogen. In STEC O157:H7 super-shedder strain SS17, a large deletion results in a smaller coding sequence, lacking the C-terminal 71 amino acids compared with Cah in STEC O157:H7 strain EDL933. We examined the function of Cah in biofilm formation and host colonization to better understand selective pressures for cah mutations...
October 20, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29038256/thiol-starvation-induces-redox-mediated-dysregulation-of-escherichia-coli-biofilm-components
#12
David A Hufnagel, Janet E Price, Rachel E Stephenson, Jesse Kelley, Matthew F Benoit, Matthew R Chapman
A hallmark of bacterial biofilms is the production of an extracellular matrix (ECM) that encases and protects the community from environmental stressors. Biofilm formation is an integral portion of the uropathogenic Escherichia coli (UPEC) lifecycle. Approximately 2% of UPEC are cysteine auxotrophs. Here, we investigated how cysteine homeostasis impacted UPEC UTI89 strain biofilm formation and, specifically, the production of the ECM components curli and cellulose. Cysteine auxotrophs produced less cellulose and slightly more curli compared to wildtype (WT) strains, and cysteine auxotrophs formed smooth, non-rugose colonies...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29016293/molecular-and-phenotypic-characterization-of-diarrheagenic-escherichia-coli-strains-isolated-from-bacteremic-children
#13
Maribel Riveros, Wilfredo García, Coralith García, David Durand, Erik Mercado, Joaquim Ruiz, Theresa J Ochoa
Escherichia coli is an important cause of Gram-negative bacteremia. The aim of this study was to characterize at the molecular and phenotypic levels E. coli strains belonging to different diarrheagenic pathotypes [diarrheagenic E. coli (DEC)] isolated from bacteremia in children younger than 5 years of age. Seventy bacteremia E. coli strains were collected in a prospective study in 12 hospitals in Lima, Peru. The presence of virulence genes associated with DEC [enterotoxigenic (lt and st), enteropathogenic (eaeA), shiga toxin-producing (stx1and stx2), enteroinvasive (ipaH), enteroaggregative (aggR), and diffusely adherent (daaD)] was determined by multiplex real-time polymerase chain reaction (PCR)...
October 9, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28986371/genotypic-and-phenotypic-characteristics-in-association-with-biofilm-formation-in-different-pathotypes-of-human-clinical-escherichia-coli-isolates
#14
Juliane Schiebel, Alexander Böhm, Jörg Nitschke, Michał Burdukiewicz, Jörg Weinreich, Aamir Ali, Dirk Roggenbuck, Stefan Rödiger, Peter Schierack
Bacterial biofilm formation is a widespread phenomenon and a complex process requiring a set of genes facilitating the initial adhesion, maturation, production of extracellular polymeric matrix (EPS) and subsequent dispersal of bacteria. Most studies on Escherichia coli (E. coli) biofilm formation have investigated non-pathogenic E. coli K-12 strains. Due to the extensive focus on laboratory strains in most studies, there is poor information regarding biofilm formation by pathogenic E. coli isolates. In this study, we genotypically and phenotypically characterized 187 human clinical E...
October 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28973027/plant-expressed-pyocins-for-control-of-pseudomonas-aeruginosa
#15
Šarūnas Paškevičius, Urtė Starkevič, Audrius Misiūnas, Astra Vitkauskienė, Yuri Gleba, Aušra Ražanskienė
The emergence, persistence and spread of antibiotic-resistant human pathogenic bacteria heralds a growing global health crisis. Drug-resistant strains of gram-negative bacteria, such as Pseudomonas aeruginosa, are especially dangerous and the medical and economic burden they impose underscore the critical need for finding new antimicrobials. Recent studies have demonstrated that plant-expressed bacteriocins of the colicins family can be efficient antibacterials against all major enteropathogenic strains of E...
2017: PloS One
https://www.readbyqxmd.com/read/28968533/using-the-agricultural-environment-to-select-better-surrogates-for-foodborne-pathogens-associated-with-fresh-produce
#16
Kimberly L Cook, Ethan C Givan, Holly M Mayton, Rohan R Parekh, Ritchie Taylor, Sharon L Walker
Despite continuing efforts to reduce foodborne pathogen contamination of fresh produce, significant outbreaks continue to occur. Identification of appropriate surrogates for foodborne pathogens facilitates relevant research to identify reservoirs and amplifiers of these contaminants in production and processing environments. Therefore, the objective of this study was to identify environmental Escherichia coli isolates from manures (poultry, swine and dairy) and surface water sources with properties similar to those of the produce associated foodborne pathogens E...
December 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28943153/melittin-and-its-potential-in-the-destruction-and-inhibition-of-the-biofilm-formation-by-staphylococcus-aureus-escherichia-coli-and-pseudomonas-aeruginosa-isolated-from-bovine-milk
#17
Tony Picoli, Cristina Mendes Peter, João Luíz Zani, Stefanie Bressan Waller, Matheus Gomes Lopes, Kamilla Neutzling Boesche, Gilberto D Ávila Vargas, Silvia de Oliveira Hübner, Geferson Fischer
Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa stand out in veterinary and human medicine for their role in opportunistic infections and their pathogenic mechanisms, including the biofilms formation. It was investigated the antibacterial activity of melittin and antibiofilm of such bacteria. Twelve strains of these microorganisms isolated from bovine milk were used, as well as the strains S. aureus ATCC 12600, E. coli ATCC 8739 and Pseudomonas aeruginosa ATCC 15442. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution technique...
September 21, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28927409/biological-and-physicochemical-properties-of-biosurfactants-produced-by-lactobacillus-jensenii-p6a-and-lactobacillus-gasseri-p65
#18
I M C Morais, A L Cordeiro, G S Teixeira, V S Domingues, R M D Nardi, A S Monteiro, R J Alves, E P Siqueira, V L Santos
BACKGROUND: Lactobacillus species produce biosurfactants that can contribute to the bacteria's ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. Here, we described the biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. RESULTS: The biosurfactants produced by L. jensenii P6A and L. gasseri P65 reduced the water surface tension from 72 to 43...
September 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28922801/effect-of-severe-weather-events-on-the-shedding-of-shiga-toxigenic-escherichia-coli-in-slaughter-cattle-and-phenotype-of-serogroup-o157-isolates
#19
Kim Stanford, Tim Reuter, Susan J Bach, Linda Chui, Angela Ma, Cheyenne C Conrad, Renata Tostes, Tim A McAllister
High-event periods (HEPs) occur sporadically when beef carcasses and meat have episodes of acute contamination with Shiga toxin-producing Escherichia coli (STEC). In this study, severe weather events were investigated as catalysts for HEPs based on PCR and isolate prevalence of seven E. coli serogroups in slaughter cattle feces. Winter ambient temperatures with daily means 10.5oC warmer or 12.3°C colder than seasonal norms (-10.4°C) most altered STEC shedding. Fecal samples yielded increased proportions (P < 0...
September 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28894939/identification-and-characterization-of-novel-broad-spectrum-amino-acid-racemases-from-escherichia-coli-and-bacillus-subtilis
#20
Tetsuya Miyamoto, Masumi Katane, Yasuaki Saitoh, Masae Sekine, Hiroshi Homma
The peptidoglycan layer of the bacterial cell wall typically contains D-alanine (D-Ala) and D-glutamic acid (D-Glu), and also various non-canonical D-amino acids that have been linked to peptidoglycan remodeling, inhibition of biofilm formation, and triggering of biofilm disassembly. Bacteria produce D-amino acids when adapting to environmental changes as a common survival strategy. In our previous study, we detected non-canonical D-amino acids in Escherichia coli grown in minimal medium. However, the biosynthetic pathways of non-canonical D-amino acids remain poorly understood...
November 2017: Amino Acids
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