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bacteriological water analysis

Aneela Zameer Durrani, Zubair Bashir, Imran Rasheed, Noor-Ul-Ain Sarwar
Bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in Cholistan, Dera Ismail Khan and Rahim Yar Khan districts in South Punjab, Pakistan. For this purpose 300 camels were sampled randomly at different common grazing and watering point. For study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. An overall prevalence of 15%and 5% was recorded for trypanosomiasis and Anaplasmosis respectively. Trypanosoma evansi was identified with 280 bp product on polymerase chain reaction test...
April 18, 2017: Microbial Pathogenesis
Robert Wolf, Jörg Hiesel, Sabrina Kuchling, Armin Deutz, John Kastelic, Herman W Barkema, Peter Wagner
Clostridium chauvoei is a gram positive, spore building bacterium that causes blackleg, a mostly fatal disease in cattle and other ruminants. Although the disease is common, little is known on the epidemiology of blackleg. As infection occurs through the environment, the risk of blackleg might be increased in areas with a specific climate or soil type. Therefore, the objective of the present study was to identify spatial and temporal clusters in the incidence of blackleg in the province of Styria, Austria. Data were collected within the governmentally delivered blackleg control program which includes vaccination of cattle with access to known blackleg pastures and compensation for fallen stock...
March 1, 2017: Preventive Veterinary Medicine
Andrew P Jacobson, Hua Wang, Vikas S Gill, Robert Duvall, Gabriela Arce, Stuart Chirtel, Thomas S Hammack
Four buffered preenrichment media (BAX(®) System MP Media (BAX)), Universal Preenrichment Broth (UPB), modified Buffered Peptone Water (mBPW), and Buffered Peptone Water (BPW)) were compared with lactose broth (LB) in the Bacteriological Analytical Manual's (BAM) Salmonella culture method for the analysis of 9 leafy green produce and herb types. Artificially contaminated test portions were pre-enriched in each medium and the results were analyzed statistically using Fisher's Exact 2-tailed F test (p < 0...
May 2017: Food Microbiology
Julia Krolik, Allison Maier, Shawna Thompson, Anna Majury
Many people living in rural areas rely on privately owned wells as their primary source of drinking water. These water sources are at risk for fecal contamination of human, wildlife, and livestock origin. While traditional bacteriological testing involves culture-based methods, microbial source tracking (MST) assays present an opportunity to additionally determine the source of fecal contamination. This study investigated the main host sources of contamination in private well water samples with high levels of Escherichia coli (E...
December 2016: Journal of Water and Health
Željko Cvetnić, Sanja Duvnjak, Martina Đuras, Tomislav Gomerčić, Irena Reil, Maja Zdelar-Tuk, Silvio Špičić
Marine mammal brucellosis has been known for more than 20 years, but recent work suggests it is more widespread than originally thought. Brucella (B.) pinnipedialis has been isolated from pinnipeds, while B. ceti strains have been associated with cetaceans. Here we report a Brucella strain isolated from multiple lymph nodes of one bottlenose dolphin (Tursiops truncatus) during routine examination of dolphin carcasses found in the Croatian part of the northern Adriatic Sea during the summer of 2015. Classical bacteriological biotyping, PCR-based techniques (single, multiplex, PCR-RFLP) and 16S rRNA DNA sequencing were used to identify Brucella spp...
November 30, 2016: Veterinary Microbiology
Pitchouna I Kilunga, John M Kayembe, Amandine Laffite, Florian Thevenon, Naresh Devarajan, Crispin K Mulaji, Josué I Mubedi, Zéphirin G Yav, Jean-Paul Otamonga, Pius T Mpiana, John Poté
Although the United Nations General Assembly recognized in 2010 the right to safe and clean drinking water and sanitation as a human right that is essential to the full enjoyment of life and all other human rights, the contamination of water supplies with faecal pathogens is still a major and unsolved problem in many parts of the world. In this study, faecal indicator bacteria (FIB), including Escherichia coli (E. coli) and Enterococcus (ENT), were quantified over the period of June/July 2014 and June/July 2015 to assess the quality of hospital effluents (n = 3: H1, H2 and H3) and of rivers receiving wastewaters from the city of Kinshasa, Democratic Republic of Congo...
October 14, 2016: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Ghada Masoud, Aleya Abbass, Amani Abaza, Walaa Hazzah
Swimming pools have been identified as posing some public health risks to users due to either bacterial or chemical contamination. As a result, maintaining good swimming pool water quality is an important issue in preventing health risks for bathers. This study aimed to evaluate the bacteriological quality of some swimming pools in Alexandria and to investigate the prevalence of Staphylococcus aureus (S. aureus) in water samples. A total of 120 water samples from 10 swimming pools were collected. Bacteriological analysis included heterotrophic plate count (HPC) using pour plate method; enumeration of total coliforms (TC), Escherichia coli (E...
July 2016: Environmental Monitoring and Assessment
G B N Assis, G C Tavares, F L Pereira, H C P Figueiredo, C A G Leal
Streptococcus agalactiae and Francisella noatunensis subsp. orientalis (Fno) are important pathogens for farm-raised tilapia worldwide. There are no reports of coinfection caused by S. agalactiae and Fno in fish. This study aimed to determine the aetiology of atypical mortalities in a cage farm of Nile tilapia and to characterize the genetic diversity of the isolates. Fifty-two fish were sampled and subjected to parasitological and bacteriological examination. The S. agalactiae and Fno isolates were genotyped using MLST and REP-PCR, respectively...
January 2017: Journal of Fish Diseases
Mayada R Moussa, Rim A Hussein, Hesham M El-Naggar
BACKGROUND: According to the WHO, nearly 24% of the global disease burden is attributable to environmental risk factors (RFs). People living in periurban areas are particularly at risk due to lack of basic sanitation requirements. OBJECTIVE: To investigate the possible association between environmental RFs and disease prevalence for a community living in a periurban area in Alexandria, Egypt. MATERIALS AND METHODS: A cross-sectional study was conducted in 2012...
March 2016: Journal of the Egyptian Public Health Association
Amanjot K Singh, Vikram Kumar Gupta, Bhuvan Sharma, Bhavna Singla, Paramjeet Kaur, Geeta Walia
INTRODUCTION: Ground water is the ultimate and most suitable fresh water resource for human consumption in the urban areas of India. Studies regarding ground water quality have shown that the higher rate of exploration as compared to the rate of recharging, inappropriate dumping of solid, as well as liquid waste, lack of strict enforcement of law has led to the deterioration of ground water quality. The present study was thus, carried out to evaluate physicochemical, as well as a microbiological profile of tap water, and filtered water in urban areas of Patiala, Punjab...
October 2015: Journal of Family Medicine and Primary Care
Pelin Yilmaz, Pablo Yarza, Josephine Z Rapp, Frank O Glöckner
Determining which microbial taxa are out there, where they live, and what they are doing is a driving approach in marine microbial ecology. The importance of these questions is underlined by concerted, large-scale, and global ocean sampling initiatives, for example the International Census of Marine Microbes, Ocean Sampling Day, or Tara Oceans. Given decades of effort, we know that the large majority of marine Bacteria and Archaea belong to about a dozen phyla. In addition to the classically culturable Bacteria and Archaea, at least 50 "clades," at different taxonomic depths, exist...
2015: Frontiers in Microbiology
Abhilasha Karkey, Thibaut Jombart, Alan W Walker, Corinne N Thompson, Andres Torres, Sabina Dongol, Nga Tran Vu Thieu, Duy Pham Thanh, Dung Tran Thi Ngoc, Phat Voong Vinh, Andrew C Singer, Julian Parkhill, Guy Thwaites, Buddha Basnyat, Neil Ferguson, Stephen Baker
One of the UN sustainable development goals is to achieve universal access to safe and affordable drinking water by 2030. It is locations like Kathmandu, Nepal, a densely populated city in South Asia with endemic typhoid fever, where this goal is most pertinent. Aiming to understand the public health implications of water quality in Kathmandu we subjected weekly water samples from 10 sources for one year to a range of chemical and bacteriological analyses. We additionally aimed to detect the etiological agents of typhoid fever and longitudinally assess microbial diversity by 16S rRNA gene surveying...
January 2016: PLoS Neglected Tropical Diseases
Pasqualina Laganà, Umberto Moscato, Andrea Poscia, Daniele Ignazio La Milia, Stefania Boccia, Emanuela Avventuroso, Santi Delia
INTRODUCTION: Legionnaires' disease is normally acquired by inhalation of legionellae from a contaminated environmental source. Water systems of large buildings, such as hospitals, are often contaminated with legionellae and therefore represent a potential risk for the hospital population. The aim of this study was to evaluate the potential contamination of Legionella pneumophila (LP) in a large hospital in Italy through georeferential statistical analysis to assess the possible sources of dispersion and, consequently, the risk of exposure for both health care staff and patients...
2015: Annals of Agricultural and Environmental Medicine: AAEM
Michał Michałkiewicz, Dobrochna Ginter-Kramarczyk, Izabela K Kruszelnicka
BACKGROUND: Water supplied to the dental units must be of sufficient quality. The article presents the results of the microbiological analysis of cold municipal water which flows into a patient's disposable mouthwash cup, and demineralized water which flows through a waterline into the tool panel of a dental unit from the tank placed in the water group. MATERIAL AND METHODS: In order to assess the degree of purity (impurities) of water used in dental units, 2 series of microbiological tests were carried out in 6 dental surgeries from April to June, 2013...
2015: Medycyna Pracy
Suma George Mulamattathil, Carlos Bezuidenhout, Moses Mbewe
Mafikeng, the capital of the North West Province, receives water from two sources, namely the Molopo eye and the Modimola dam. Once treated, the potable water is mixed and supplied to the city via distribution systems. This study was designed to assess the quality of drinking water in Mafikeng and also to determine whether the water from the two sources has an impact on the mixed water quality. Physico-chemical parameters and bacteriological quality (faecal coliforms (FCs), total coliforms (TCs), heterotrophic bacteria and Peudomonas spp...
December 2015: Journal of Water and Health
Shiva Verma, V A Indumathi, K C Gurudev, Shalini Ashok Naik
INTRODUCTION: Haemodialysis is one of the treatment modalities for patients suffering from end stage renal disease (ESRD). Dialysis patients are exposed to large volumes of water for production of dialysis fluids. Treated water and dialysate come in direct contact with the patient's bloodstream. Such patients suffer from abnormalities of the immune system, making them more susceptible to infections. Microbial contamination of the treated water and dialysate can lead to biofilm formation and release of endotoxins in Haemodialysis system...
October 2015: Journal of Clinical and Diagnostic Research: JCDR
Olumide A Odeyemi
BACKGROUND: Over the past years, there has been increase in packaged water consumption in Nigeria. Although, there are several studies on microbial safety of sachet packaged drinking water, there is no information on prevailing pathogens. FINDINGS: A comprehensive literature search and meta-analysis of peer reviewed primary studies reported from 2005 for microbiological safety of packaged drinking water sold in Nigeria was conducted using "sachet water", "bottled water" and "packaged water" and Nigeria as search algorithms in public scientific literature databases...
2015: SpringerPlus
Paul N Waithaka, John Muthini Maingi, Anthony Kebira Nyamache
Nakuru North sub-county is a peri-urban area which has both dry and wet seasons. Its residents rely mostly on untreated water sources for daily water needs due to unreliable water supply from the urban council. However, this water has not been evaluated on its quality despite residents solely depending on it. This study was aimed at determining the physico-chemical and bacteriological quality of water drawn from River Kandutura and water taps in Nakuru North sub-county. In addition, the study was aimed at carrying out sensitivity test of the isolates to antibiotics and determining effectiveness of solar disinfection in water treatment...
2015: Open Microbiology Journal
Rogerio Tiyo, Carla Zangari de Souza, Letícia Nishi, Camila Fernanda Brustolin, Bianca Altrão Ratti, Ana Lucia Falavigna Guilherme
The aim of this work was to compare, from a parasitological ( Cryptosporidium spp. and Giardia duodenalis), bacteriological (total and thermotolerants coliforms) and physicochemical perspective, water sources used for drinking and irrigation of vegetables intended to be sold for human consumption. From January 2010 to May 2011, samples of different water sources from vegetable producing properties were collected; 100 liters for parasitological analysis, 200 mL for bacteriological analysis, and five liters for physicochemical analysis...
July 2015: Revista do Instituto de Medicina Tropical de São Paulo
Amen Gumbo, Donewell Bangure, Notion T Gombe, More Mungati, Mufuta Tshimanga, Zanele Hwalima, Ignatious Dube
BACKGROUND: Bulawayo City Council held an Integrated Result Based Management workshop among 86 employees from August 18-22, 2014 at Ikhwezi Training Centre in Bulawayo City. On August 21, 2014, a report of diarrhoea among Council employees attending the workshop was received. We investigated the outbreak to determine the risk factors associated with diarrhoea at Ikhwezi Training Centre, Bulawayo City. METHOD: A retrospective cohort study was conducted where 74 Council employees were interviewed on food consumed and presenting signs and symptoms...
2015: BMC Research Notes
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