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shiga toxin producing enteropathogenic E. coli

Lutz Geue, Christian Menge, Christian Berens, Stefanie A Barth
Shiga toxin-producing Escherichia coli (STEC) strains are important zoonotic enteric pathogens with the main reservoir in cattle. Here, we present the genomes of two STEC strains and one atypical enteropathogenic E. coli strain from cattle origin, obtained during a longitudinal study in German cattle herds.
May 11, 2017: Genome Announcements
Atieh Darbandi, Parviz Owlia, Saeid Bouzari, Horieh Saderi
BACKGROUND AND OBJECTIVES: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), diffusely adhering E. coli (DAEC) and shiga toxin producing E. coli (STEC) strains are among the most frequent causative agents in acute diarrhea. The aim of this study was to determine the frequency of DEC pathotypes in Khuzestan province...
December 2016: Iranian Journal of Microbiology
Lili Wang, Shaobo Zhang, Dongming Zheng, Sami Fujihara, Akiyo Wakabayashi, Kazuyuki Okahata, Masakazu Suzuki, Atsunori Saeki, Hiromi Nakamura, Yukiko Hara-Kudo, Eriko Kage-Nakadai, Yoshikazu Nishikawa
The source and routes of diarrheagenic Escherichia coli (DEC) remain poorly clarified. To investigate the involvement of domestic animals in the dissemination of DEC, the prevalence of DEC among foods and fecal specimens of cattle, pigs, chickens, healthy carriers, and patients in Osaka and Hyogo, Japan, was investigated using a multiplex real-time PCR assay. The most prevalent virulence genes were astA and eae, with prevalences of 46.8% and 27.4%, respectively. stx1 (26.6%) and stx2 (45.9%) were prevalent in cattle feces, and est (8...
March 28, 2017: Japanese Journal of Infectious Diseases
Srikanth Palanisamy, YuChen Chang, Joy Scaria, Rafael Antonio Casarin Penha Filho, Kenlyn E Peters, Sanjay H Doiphode, Ali Sultan, Hussni O Mohammed
INTRODUCTION: Pathogenic Escherichia coli has been listed among the most important bacteria associated with foodborne illnesses around the world. We investigated the genetic relatedness among Shiga toxin-producing E. coli (STEC) isolated along the animal food supply chain and from humans diagnosed with gastroenteritis in Qatar. METHODS: Samples were collected from different sources along the food supply chain and from patients admitted to the hospital with complaints of gastroenteritis...
March 30, 2017: Foodborne Pathogens and Disease
Lili Wang, Hiromi Nakamura, Eriko Kage-Nakadai, Yukiko Hara-Kudo, Yoshikazu Nishikawa
Diarrheagenic E. coli (DEC) isolates were recovered from local retail markets and the Osaka Municipal Central Wholesale Market in Japan. Retail food samples were collected for analysis in Osaka Japan from 2005 to 2008 and consisted of 32 beef, 28 pork, 20 poultry, 136 fish, 66 fruits and vegetables and 51 ready-to-eat (RTE) food samples. A total of 82 DEC strains were recovered from 64 (19%) food samples with the highest prevalence in poultry (100%, 20/20), followed by pork (54%, 15/28), beef (28%, 9/32), fruits and vegetables (12%, 8/66), fish (6...
March 7, 2017: International Journal of Food Microbiology
Esmeralda Rangel-Vargas, Carlos A Gómez-Aldapa, Reyna N Falfan-Cortes, María L Rodríguez-Marín, Angélica Godínez-Oviedo, Otilio A Acevedo-Sandoval, Javier Castro-Rosas
Chili peppers are a very important crop in Mexico. However, these peppers have been associated with Salmonella infection outbreaks in the United States, and Salmonella and diarrheagenic Escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in Mexico. To decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. Roselle ( Hibiscus sabdariffa ) calyces have been reported to have an antimicrobial effect on pathogenic bacteria...
February 15, 2017: Journal of Food Protection
Mithila Ferdous, Anna M D Kooistra-Smid, Kai Zhou, John W A Rossen, Alexander W Friedrich
Escherichia coli (E.coli) O157 that do not produce Shiga toxin and do not possess flagellar antigen H7 are of diverse H serotypes. In this study, the antibiotic resistance properties, genotype of a set of virulence associated genes and the phylogenetic background of E. coli O157:non-H7 groups were compared. Whole genome sequencing was performed on fourteen O157:non-H7 isolates collected in the STEC-ID-net study. The genomes were compared with E. coli O157 genomes and a typical Enteropathogenic E. coli (tEPEC) genome downloaded from NCBI...
2016: Frontiers in Microbiology
Rowan R Thomas, Heather J L Brooks, Rory O'Brien
BACKGROUND: Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli are gastrointestinal pathogens causing diarrhoeal and extraintestinal disease. Due to lack of EPEC screening and use of Sorbitol-MacConkey (SMAC) agar in faecal screening, the true prevalence of EPEC and non-O157 STEC in New Zealand diarrhoeal cases is unknown. METHODS: Diarrhoeic stools sourced from Dunedin hospital were pre-enriched, DNA extracted with Chelex-100 resin and screened using a multiplex TaqMan quantitative PCR assay amplifying stx1, sxt2 and EPEC (eae) gene markers...
January 2017: Journal of Clinical Pathology
Carlos A Gómez-Aldapa, Jesús A Segovia-Cruz, Jorge F Cerna-Cortes, Esmeralda Rangel-Vargas, Laura P Salas-Rangel, Eduardo J Gutiérrez-Alcántara, Javier Castro-Rosas
The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C...
October 2016: Food Microbiology
K De Rauw, L Detemmerman, J Breynaert, D Piérard
The purpose of this investigation was the evaluation of the performance of the BioFire FilmArray® Gastrointestinal (FA-GI) Panel, a multiplexed molecular stool screening assay, for the detection of diarrheagenic Escherichia coli (DEC), with emphasis on Shiga toxin-producing E. coli (STEC). A dilution series of 12 STEC reference strains was tested with the FA-GI Panel to assess the analytical sensitivity. A total of 389 patient samples were analyzed with the FA-GI Panel and confirmation of the detected DEC was attempted with an in-house culture-based polymerase chain reaction (PCR) method...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
Karla Vasco, Jay P Graham, Gabriel Trueba
UNLABELLED: Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in Ecuador between June and August 2014. Multilocus sequence typing (MLST) was used to assess zoonotic transmission of Campylobacter jejuni and atypical enteropathogenic Escherichia coli (aEPEC), which were the most prevalent bacterial pathogens in children and domestic animals (30...
July 15, 2016: Applied and Environmental Microbiology
A Mahanti, I Samanta, S Bandyopadhyay, S N Joardar
The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx 1/stx 2) was detected in 36 (14.7%) of the 245 E. coli isolates. These STEC strains belonged to 22 different serogroups (O2, O5, O20, O21, O22, O25, O41, O44, O45, O60, O71, O76, O84, O85, O87, O91, O103, O112, O113, O120, O156, and O158) and three were untypeable...
2015: Iranian journal of veterinary research
Zheng Huang, Haijian Pan, Pingping Zhang, Xiaowei Cao, Wenting Ju, Chuanqing Wang, Jianmin Zhang, Jianghong Meng, Zhengan Yuan, Xuebin Xu
BACKGROUND: Diarrheagenic Escherichia coli (DEC) is a major cause of bacterial gastroenteritis in children. Also, antibiotic resistance among DEC is becoming a critical area of concern in clinical settings. METHODS: This study was conducted in 4 hospitals in Shanghai from June 2012 to October 2013. DEC isolates from stool samples of patients with diarrhea were examined to determine their antimicrobial susceptibilities and presence of virulence genes, in order to identify high risk clones...
August 2016: Pediatric Infectious Disease Journal
C Balière, A Rincé, S Delannoy, P Fach, M Gourmelon
UNLABELLED: Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively...
July 1, 2016: Applied and Environmental Microbiology
Rouhollah Kazemi, Asal Akhavian, Jafar Amani, Jafar Salimian, Mohammad-Javad Motamedi, Amir Mousavi, Mahyat Jafari, Ali-Hatef Salmanian
Infectious diarrhoea remains an emerging problem in the world health program. Among diarrheagenic agents, Vibrio cholerae and enterotoxigenic and enterohemorrhagic Escherichia coli are critical enteropathogens. AB5 toxin produced by these bacteria, heat-labile enterotoxin (LT), cholera enterotoxin (CT), and shiga-like cytotoxin (STX) can target the immune system and are subunit vaccine candidates. A chemically-synthesized chimeric construct composed of the binding subunits of these toxins (LTB, STXB, and CTXB) was developed based on bioinformatics studies...
June 2016: Microbes and Infection
Carlos A Gómez-Aldapa, Jorge F Cerna-Cortes, Esmeralda Rangel-Vargas, Mdel Refugio Torres-Vitela, Angelica Villarruel-López, Eduardo J Gutiérrez-Alcántara, Javier Castro-Rosas
The presence of multidrug-resistant pathogenic bacteria in food is a significant public health concern. Diarrheagenic Escherichia coli pathotypes (DEPs) are foodborne bacteria. In Mexico, DEPs have been associated with diarrheal illness. There is no information about the presence of multidrug-resistant DEPs on fresh vegetables and in cooked vegetable salads in Mexico. "Nopalitos" (Opuntia ficus-indica L.) is a Cactacea extensively used as a fresh green vegetable throughout Mexico. The presence of generic E...
May 2016: Foodborne Pathogens and Disease
I Fakih, D Thiry, J-N Duprez, M Saulmont, A Iguchi, D Piérard, L Jouant, G Daube, Y Ogura, T Hayashi, B Taminiau, J G Mainil
Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www...
April 2017: Veterinary Microbiology
Hong Tang, Yong Li, Pingping Zhang, Jiayin Guo, Zheng Huang, Hao Xu, Qi Hou, Chuanqing Wang, Mei Zeng, Huiming Jin, Jiayu Hu, Xianming Shi, Jianmin Zhang, Biao Kan, Yanwen Xiong, Ailan Zhao, Lu Ran, Xuebin Xu
OBJECTIVE: To understand the distribution of diarrheagenic Escherichia (E.) coli in population in Shanghai and discuss the practice model of cooperation in enteric infectious disease prevention and control between public health institution and hospital. METHODS: Sentinel hospitals were assigned, standard detection and identification of diarrheagenic E. coli were conducted, incidence curve of diarrheagenic E. coli infection was drawn and epidemiologic survey and laboratory detection were conducted for suspect diarrheagenic E...
November 2015: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Fatemeh Doregiraee, Masoud Alebouyeh, Bahar Nayeri Fasaei, Saeed Charkhkar, Elahe Tajedin, Mohammad Reza Zali
AIM: The purpose of this study was to investigate the prevalence of enteropathogenic Escherichia coli (EPEC) and shiga toxin producing E. coli (STEC) strains in healthy broilers in Iran. BACKGROUND: STEC and EPEC strains as diarrheagenic E. coli are among the most prevalent causative agents in acute diarrhea. Domestic animals, mainly cattle and sheep, have been implicated as the principal reservoirs of these pathotypes; however their prevalence among the broilers is varied among different countries...
2016: Gastroenterology and Hepatology From Bed to Bench
S Baron, S Delannoy, S Bougeard, E Larvor, E Jouy, O Balan, P Fach, I Kempf
This study investigated antimicrobial resistance, screened for the presence of virulence genes involved in intestinal infections, and determined phylogenetic groups of Escherichia coli isolates from untreated poultry and poultry treated with ceftiofur, an expanded-spectrum cephalosporin. Results show that none of the 76 isolates appeared to be Shiga toxin-producing E. coli or enteropathogenic E. coli. All isolates were negative for the major virulence factors/toxins tested (ehxA, cdt, heat-stable enterotoxin [ST], and heat-labile enterotoxin [LT])...
December 14, 2015: Antimicrobial Agents and Chemotherapy
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