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shiga toxin producing enteropathogenic E. coli

Mithila Ferdous, Anna M D Kooistra-Smid, Kai Zhou, John W A Rossen, Alexander W Friedrich
Escherichia coli (E.coli) O157 that do not produce Shiga toxin and do not possess flagellar antigen H7 are of diverse H serotypes. In this study, the antibiotic resistance properties, genotype of a set of virulence associated genes and the phylogenetic background of E. coli O157:non-H7 groups were compared. Whole genome sequencing was performed on fourteen O157:non-H7 isolates collected in the STEC-ID-net study. The genomes were compared with E. coli O157 genomes and a typical Enteropathogenic E. coli (tEPEC) genome downloaded from NCBI...
2016: Frontiers in Microbiology
Rowan R Thomas, Heather J L Brooks, Rory O'Brien
BACKGROUND: Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli are gastrointestinal pathogens causing diarrhoeal and extraintestinal disease. Due to lack of EPEC screening and use of Sorbitol-MacConkey (SMAC) agar in faecal screening, the true prevalence of EPEC and non-O157 STEC in New Zealand diarrhoeal cases is unknown. METHODS: Diarrhoeic stools sourced from Dunedin hospital were pre-enriched, DNA extracted with Chelex-100 resin and screened using a multiplex TaqMan quantitative PCR assay amplifying stx1, sxt2 and EPEC (eae) gene markers...
October 3, 2016: Journal of Clinical Pathology
Carlos A Gómez-Aldapa, Jesús A Segovia-Cruz, Jorge F Cerna-Cortes, Esmeralda Rangel-Vargas, Laura P Salas-Rangel, Eduardo J Gutiérrez-Alcántara, Javier Castro-Rosas
The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C...
October 2016: Food Microbiology
K De Rauw, L Detemmerman, J Breynaert, D Piérard
The purpose of this investigation was the evaluation of the performance of the BioFire FilmArray® Gastrointestinal (FA-GI) Panel, a multiplexed molecular stool screening assay, for the detection of diarrheagenic Escherichia coli (DEC), with emphasis on Shiga toxin-producing E. coli (STEC). A dilution series of 12 STEC reference strains was tested with the FA-GI Panel to assess the analytical sensitivity. A total of 389 patient samples were analyzed with the FA-GI Panel and confirmation of the detected DEC was attempted with an in-house culture-based polymerase chain reaction (PCR) method...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
Karla Vasco, Jay P Graham, Gabriel Trueba
UNLABELLED: Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in Ecuador between June and August 2014. Multilocus sequence typing (MLST) was used to assess zoonotic transmission of Campylobacter jejuni and atypical enteropathogenic Escherichia coli (aEPEC), which were the most prevalent bacterial pathogens in children and domestic animals (30...
July 15, 2016: Applied and Environmental Microbiology
A Mahanti, I Samanta, S Bandyopadhyay, S N Joardar
The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx 1/stx 2) was detected in 36 (14.7%) of the 245 E. coli isolates. These STEC strains belonged to 22 different serogroups (O2, O5, O20, O21, O22, O25, O41, O44, O45, O60, O71, O76, O84, O85, O87, O91, O103, O112, O113, O120, O156, and O158) and three were untypeable...
2015: Iranian journal of veterinary research
Zheng Huang, Haijian Pan, Pingping Zhang, Xiaowei Cao, Wenting Ju, Chuanqing Wang, Jianmin Zhang, Jianghong Meng, Zhengan Yuan, Xuebin Xu
BACKGROUND: Diarrheagenic Escherichia coli (DEC) is a major cause of bacterial gastroenteritis in children. Also, antibiotic resistance among DEC is becoming a critical area of concern in clinical settings. METHODS: This study was conducted in 4 hospitals in Shanghai from June 2012 to October 2013. DEC isolates from stool samples of patients with diarrhea were examined to determine their antimicrobial susceptibilities and presence of virulence genes, in order to identify high risk clones...
August 2016: Pediatric Infectious Disease Journal
C Balière, A Rincé, S Delannoy, P Fach, M Gourmelon
UNLABELLED: Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively...
July 1, 2016: Applied and Environmental Microbiology
Rouhollah Kazemi, Asal Akhavian, Jafar Amani, Jafar Salimian, Mohammad-Javad Motamedi, Amir Mousavi, Mahyat Jafari, Ali-Hatef Salmanian
Infectious diarrhoea remains an emerging problem in the world health program. Among diarrheagenic agents, Vibrio cholerae and enterotoxigenic and enterohemorrhagic Escherichia coli are critical enteropathogens. AB5 toxin produced by these bacteria, heat-labile enterotoxin (LT), cholera enterotoxin (CT), and shiga-like cytotoxin (STX) can target the immune system and are subunit vaccine candidates. A chemically-synthesized chimeric construct composed of the binding subunits of these toxins (LTB, STXB, and CTXB) was developed based on bioinformatics studies...
June 2016: Microbes and Infection
Carlos A Gómez-Aldapa, Jorge F Cerna-Cortes, Esmeralda Rangel-Vargas, Mdel Refugio Torres-Vitela, Angelica Villarruel-López, Eduardo J Gutiérrez-Alcántara, Javier Castro-Rosas
The presence of multidrug-resistant pathogenic bacteria in food is a significant public health concern. Diarrheagenic Escherichia coli pathotypes (DEPs) are foodborne bacteria. In Mexico, DEPs have been associated with diarrheal illness. There is no information about the presence of multidrug-resistant DEPs on fresh vegetables and in cooked vegetable salads in Mexico. "Nopalitos" (Opuntia ficus-indica L.) is a Cactacea extensively used as a fresh green vegetable throughout Mexico. The presence of generic E...
May 2016: Foodborne Pathogens and Disease
I Fakih, D Thiry, J-N Duprez, M Saulmont, A Iguchi, D Piérard, L Jouant, G Daube, Y Ogura, T Hayashi, B Taminiau, J G Mainil
Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www...
February 23, 2016: Veterinary Microbiology
Hong Tang, Yong Li, Pingping Zhang, Jiayin Guo, Zheng Huang, Hao Xu, Qi Hou, Chuanqing Wang, Mei Zeng, Huiming Jin, Jiayu Hu, Xianming Shi, Jianmin Zhang, Biao Kan, Yanwen Xiong, Ailan Zhao, Lu Ran, Xuebin Xu
OBJECTIVE: To understand the distribution of diarrheagenic Escherichia (E.) coli in population in Shanghai and discuss the practice model of cooperation in enteric infectious disease prevention and control between public health institution and hospital. METHODS: Sentinel hospitals were assigned, standard detection and identification of diarrheagenic E. coli were conducted, incidence curve of diarrheagenic E. coli infection was drawn and epidemiologic survey and laboratory detection were conducted for suspect diarrheagenic E...
November 2015: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Fatemeh Doregiraee, Masoud Alebouyeh, Bahar Nayeri Fasaei, Saeed Charkhkar, Elahe Tajedin, Mohammad Reza Zali
AIM: The purpose of this study was to investigate the prevalence of enteropathogenic Escherichia coli (EPEC) and shiga toxin producing E. coli (STEC) strains in healthy broilers in Iran. BACKGROUND: STEC and EPEC strains as diarrheagenic E. coli are among the most prevalent causative agents in acute diarrhea. Domestic animals, mainly cattle and sheep, have been implicated as the principal reservoirs of these pathotypes; however their prevalence among the broilers is varied among different countries...
2016: Gastroenterology and Hepatology From Bed to Bench
S Baron, S Delannoy, S Bougeard, E Larvor, E Jouy, O Balan, P Fach, I Kempf
This study investigated antimicrobial resistance, screened for the presence of virulence genes involved in intestinal infections, and determined phylogenetic groups of Escherichia coli isolates from untreated poultry and poultry treated with ceftiofur, an expanded-spectrum cephalosporin. Results show that none of the 76 isolates appeared to be Shiga toxin-producing E. coli or enteropathogenic E. coli. All isolates were negative for the major virulence factors/toxins tested (ehxA, cdt, heat-stable enterotoxin [ST], and heat-labile enterotoxin [LT])...
March 2016: Antimicrobial Agents and Chemotherapy
María-Elena Álvarez-Suárez, Andrés Otero, María-Luisa García-López, Jesús A Santos
The purpose of the study was to evaluate the microbiological status (mesophilic aerobic microorganism counts) of 68 samples of bulk tank goat's milk and determine the risk associated with the foodborne pathogens Staphylococcus aureus, enteropathogenic and Shiga toxin-producing Escherichia coli, and Cronobacter sakazakii. Most samples (83.8%) complied with the limits of mesophilic aerobe counts set in the European Union for milk of species other than cows. A total of 144 isolates of coagulase-positive staphylococci were characterized, and 11 (7...
December 2015: Journal of Food Protection
Moisés Caballero, Isabel Rivera, Luis M Jara, Francisco M Ulloa-Stanojlovic, Carlos Shiva
Feral pigeons (Columbia livia) live in close contact with humans and other animals. They can transmit potentially pathogenic and zoonotic agents. The objective of this study was to isolate and detect strains of diarrheagenic Escherichia coli and Campylobacter jejuni of urban feral pigeons from an area of Lima, Peru. Fresh dropping samples from urban parks were collected for microbiological isolation of E. coli strains in selective agar, and Campylobacter by filtration method. Molecular identification of diarrheagenic pathotypes of E...
September 2015: Revista do Instituto de Medicina Tropical de São Paulo
Tadasuke Ooka, Yoshitoshi Ogura, Keisuke Katsura, Kazuko Seto, Hideki Kobayashi, Kimiko Kawano, Eisuke Tokuoka, Masato Furukawa, Seiya Harada, Shuji Yoshino, Junji Seto, Tetsuya Ikeda, Keiji Yamaguchi, Kazunori Murase, Yasuhiro Gotoh, Naoko Imuta, Junichiro Nishi, Tânia A Gomes, Lothar Beutin, Tetsuya Hayashi
Escherichia albertii is a recently recognized close relative of Escherichia coli. This emerging enteropathogen possesses a type III secretion system (T3SS) encoded by the locus of enterocyte effacement, similar to enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC). Shiga toxin-producing strains have also been identified. The genomic features of E. albertii, particularly differences from other Escherichia species, have not yet been well clarified. Here, we sequenced the genome of 29 E. albertii strains (3 complete and 26 draft sequences) isolated from multiple sources and performed intraspecies and intragenus genomic comparisons...
December 2015: Genome Biology and Evolution
Xin Wang, Jing Wang, Hao Sun, Shengli Xia, Ran Duan, Junrong Liang, Yuchun Xiao, Haiyan Qiu, Guangliang Shan, Huaiqi Jing
In China, great differences in economy, social characteristics and hygiene exist between developing and developed regions. A comparative study of infectious diarrhea between two regions was needed. Three groups of diarrheal patients were collected: children ≤5 year-olds from Beijing (developed region) and Henan Province (developing region), and adults over 18 year-olds from Beijing. A questionnaire was used to survey and feces samples were examined for 16 enteropathogens. We enrolled 1422 children and 1047 adults from developed region and 755 children from developing region...
2015: PloS One
Maryse Michèle Um, Olivier Barraud, Monique Kérourédan, Margaux Gaschet, Thibault Stalder, Eric Oswald, Christophe Dagot, Marie-Cecile Ploy, Hubert Brugère, Delphine Bibbal
The goal of this study was to investigate the involvement of bovine slaughterhouse effluents and biosolids in the risk of environmental dissemination of pathogenic and antibiotic-resistant Escherichia coli. Several samples were collected from one adult cattle and one veal calf slaughterhouse wastewater treatment plant (WWTP). The treatment process had no impact on the percentage of Shiga toxin-producing E. coli (STEC) and on the percentage of atypical enteropathogenic E. coli (aEPEC). A STEC O157:H7 was isolated from the thickened sludge of the adult cattle slaughterhouse...
January 1, 2016: Water Research
Wen-Chien Huang, Bing-Mu Hsu, Po-Min Kao, Chi-Wei Tao, Ying-Ning Ho, Chun-Wei Kuo, Yu-Li Huang
Diarrheagenic Escherichia coli (DEC) are the most common agents of diarrhea. Waterborne DEC could pose a potential health risk to human through agricultural, household, recreational, and industrial use. There are few published reports on the detection of DEC and its seasonal distribution in aquatic environments. The presence of DEC in different types of aquatic environments was investigated in this study. Water samples were collected from major rivers, water reservoirs, and recreational hot springs throughout Taiwan...
February 2016: Ecotoxicology and Environmental Safety
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