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https://www.readbyqxmd.com/read/28819154/rpos-independent-evolution-reveals-the-importance-of-attenuated-camp-crp-regulation-in-high-hydrostatic-pressure-resistance-acquisition-in-e-coli
#1
Elisa Gayán, Alexander Cambré, Chris W Michiels, Abram Aertsen
High hydrostatic pressure (HHP) processing is an attractive non-thermal alternative to food pasteurization. Nevertheless, the large inter- and intra-species variations in HHP resistance among foodborne pathogens and the ease by which they can acquire extreme resistance are an issue of increasing concern. Since RpoS activity has been considered as a central determinant in the HHP resistance of E. coli and its pathovars, this study probed for the potential of an E. coli MG1655 ΔrpoS mutant to acquire HHP resistance by directed evolution...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28813002/application-of-surfactant-micelle-entrapped-eugenol-for-prevention-of-growth-of-the-shiga-toxin-producing-escherichia-coli-in-ground-beef
#2
Tamra N Tolen, Songsirin Ruengvisesh, Thomas M Taylor
Beef safety may be compromised by O157 and non-O157 Shiga toxin-producing Escherichia coli (STEC) contamination. The capacity of surfactant micelles loaded with the plant-derived antimicrobial eugenol to reduce STEC on beef trimmings that were later ground and refrigerated for five days at 5 ± 1 °C was tested to determine their utility for beef safety protection. STEC-inoculated trimmings were treated with free eugenol, micelle-encapsulated eugenol, 2% lactic acid (55 °C), sterile distilled water (25 °C), or left untreated (control)...
August 16, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28803933/phage-in-urban-wastewater-have-the-potential-to-disseminate-antibiotic-resistance
#3
Gayathri Upeksha Gunathilaka, Varun Tahlan, Abdullah Ibn Mafiz, Manisha Polur, Yifan Zhang
A total of 29 Escherichia coli phage were isolated from wastewater samples collected from an urban wastewater treatment plant (WWTP) and characterized by host range determination, Transmission Electron Microscopy (TEM), antibiotic resistance gene identification, and phage transduction. β-lactam resistance genes, blaCMY, blaTEM, blaSHV, blaCTXM, and blaOXA, were amplified on phage DNA by PCR. Out of nine host range patterns observed, six were able to multiply in three or more indicator strains, including Shiga-toxin producing E...
August 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28803013/short-communication-detection-of-stx2-and-elt-genes-in-bovine-milk-by-using-a-multiplex-pcr-system
#4
R D S Nandi, A C Campos, J J Puño-Sarmiento, R P Maluta, S P D Rocha, R K T Kobayashi, G Nakazato
The aim of this study was to detect 2 important toxin genes from diarrheagenic Escherichia coli (DEC) in bovine milk using a new multiplex PCR. To standardize the multiplex PCR, the stx2 and elt genes were investigated for the detection of Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC), respectively. The DNA template was prepared with a thermal procedure (boiling) and a commercial kit. Samples consisted of UHT and pasteurized milk, both skimmed, and STEC and ETEC were tested in concentrations between 10(1) and 10(9) cfu/mL...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28798736/escherichia-coli-o157-h7-acid-sensitivity-correlates-with-flocculation-phenotype-during-nutrient-limitation
#5
Kathryn L Kay, Frederick Breidt, Pina M Fratamico, Gian M Baranzoni, Gwang-Hee Kim, Amy M Grunden, Deog-Hwan Oh
Shiga toxin producing Escherichia coli (STEC) strains vary in acid resistance; however, little is known about the underlying mechanisms that result in strain specific differences. Among 25 STEC O157:H7 strains tested, 7 strains flocculated when grown statically for 18 h in minimal salts medium at 37°C, while 18 strains did not. Interestingly, the flocculation phenotype (cells came out of suspension) was found to correlate with degree of acid sensitivity in an assay with 400 mM acetic acid solution at pH 3...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28765569/locus-of-adhesion-and-autoaggregation-laa-a-pathogenicity-island-present-in-emerging-shiga-toxin-producing-escherichia-coli-strains
#6
David A Montero, Juliana Velasco, Felipe Del Canto, Jose L Puente, Nora L Padola, David A Rasko, Mauricio Farfán, Juan C Salazar, Roberto Vidal
Shiga Toxin-producing Escherichia coli (STEC) are a group of foodborne pathogens associated with diarrhea, dysentery, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Shiga toxins are the major virulence factor of these pathogens, however adhesion and colonization to the human intestine is required for STEC pathogenesis. A subset of STEC strains carry the Locus of Enterocyte Effacement (LEE) pathogenicity island (PAI), which encodes genes that mediate the colonization of the human intestine. While LEE-positive STEC strains have traditionally been associated with human disease, the burden of disease caused by STEC strains that lacks LEE (LEE-negative) has increased recently in several countries; however, in the absence of LEE, the molecular pathogenic mechanisms by STEC strains are unknown...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28757042/occurrence-and-characterization-of-stx-and-or-eae-positive-escherichia-coli-isolated-from-wildlife-including-a-typical-epec-strain-from-a-wild-boar
#7
Carla Andrea Alonso, Azucena Mora, Dafne Díaz, Miguel Blanco, David González-Barrio, Francisco Ruiz-Fons, Carmen Simón, Jorge Blanco, Carmen Torres
Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains are food-borne pathogens associated with acute diarrhea. Haemolytic-uremic syndrome (HUS) is often a complication of STEC infection. In order to examine the occurrence, serotypes, virulence and antimicrobial-resistance profiles of STEC and EPEC in wildlife, 326 faecal E. coli strains from 304 clinically healthy animals were analyzed. For this approach stx1, stx2 and eae genes, as well as accessory virulence determinants (ehx, hlyA, saa, tia, bfp, subAB) were PCR-screened and sequenced...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28726488/use-of-an-electrostatic-spraying-system-or-the-sprayed-lethality-in-container-method-to-deliver-antimicrobial-agents-onto-the-surface-of-beef-subprimals-to-control-shiga-toxin-producing-escherichia-coli
#8
J Max Stella, John B Luchansky, Kelsey Miller, Bradley A Shoyer, Laura E Shane, Lianna McGeary, Manuela Osoria, Laura J Stahler, Nicholas J Sevart, Randall K Phebus, Harshavardhan Thippareddi, Anna C S Porto-Fett
The efficacy of an electrostatic spraying system (ESS) and/or the sprayed lethality in container (SLIC) method to deliver antimicrobial agents onto the surface of beef subprimals to reduce levels of Shiga toxin-producing Escherichia coli (STEC) was evaluated. Beef subprimals were surface inoculated (lean side; ca. 5.8 log CFU per subprimal) with 2 mL of an eight-strain cocktail comprising single strains of rifampin-resistant (100 μg/mL) STEC (O26:H11, O45:H2, O103:H2, O104:H4, O111:H(-), O121:H19, O145:NM, and O157:H7)...
July 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28718802/ouabain-protects-human-renal-cells-against-the-cytotoxic-effects-of-shiga-toxin-type-2-and-subtilase-cytotoxin
#9
María M Amaral, Magalí C Girard, Romina S Álvarez, Adrienne W Paton, James C Paton, Horacio A Repetto, Flavia Sacerdoti, Cristina A Ibarra
Hemolytic uremic syndrome (HUS) is one of the most common causes of acute renal failure in children. The majority of cases are associated with Shiga toxin (Stx)-producing Escherichia coli (STEC). In Argentina, HUS is endemic and presents the highest incidence rate in the world. STEC strains expressing Stx type 2 (Stx2) are responsible for the most severe cases of this pathology. Subtilase cytotoxin (SubAB) is another STEC virulence factor that may contribute to HUS pathogenesis. To date, neither a licensed vaccine nor effective therapy for HUS is available for humans...
July 18, 2017: Toxins
https://www.readbyqxmd.com/read/28715078/polish-stress-echocardiography-registry-pol-stress-registry-a-multicenter-study-stress-echo-in-poland-numbers-settings-results-and-complications
#10
Edyta Płońska-Gościniak, Jarosław D Kasprzak, Szymon Olędzki, Jolanta Rzucidło-Resil, Piotr Gościniak, Tomasz Kukulski, Katarzyna Mizia-Stec, Wojciech Kosmala, Dariusz Kosior, Marta Marcinkiewicz-Siemion, Barbara Brzezińska, Beata Zaborska, Marcin Fijałkowski, Iwona Świątkiewicz, Andrzej Szyszka, Jan Błażejewski, Wojciech Witkiewicz, Zbigniew Gąsior, Danuta Sorysz, Agnieszka Olszanecka
BACKGROUND: Stress echocardiography (SE) is widely used in Europe. No collective data have been available on the use of SE in Poland until now. AIM: To evaluate the number of SE investigations performed in Poland, their settings, complications and results. METHODS: In this retrospective survey, referral cardiology centers in Poland were asked to fill in a questionnaire regarding SE examinations performed from May 01, 2014 to May 01, 2015. RESULTS: The study included data from 17 university hospitals and large community hospitals which performed 4611 SE examinations, including 4408 tests in patients investigated for coronary artery disease (CAD) and 203 tests to evaluate valvular heart disease (VHD)...
July 17, 2017: Kardiologia Polska
https://www.readbyqxmd.com/read/28713792/shiga-toxin-producing-escherichia-coii-in-slaughtered-pigs-and-pork-products
#11
Lia Bardasi, Roberta Taddei, Ilaria Fiocchi, Maria Francesca Pelliconi, Mattia Ramini, Elena Toschi, Giuseppe Merialdi
During the years 2015-2016, 83 faecal samples were collected at slaughter from pigs reared in farms located in Central-Northern Italy. During the years 2014-2016 a total of 562 pork products [465 not-readyto-eat (NRTE) and 97 ready-to-eat (RTE) products] were collected from retail outlets, large retailers and processing plants. The samples were analysed according to ISO TS 13136:2012. Out of 83 swine faecal samples, 77 (92.8%) resulted stx-positive by real time polymerase chain reaction (PCR), 5 stx2+ and 1 stx1+ Shiga toxin-producing Escherichia coli (STEC) strains were isolated...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28687651/characterization-of-shiga-toxin-producing-escherichia-coli-strains-of-o91-serogroup-isolated-from-food-and-environmental-samples
#12
Peter C H Feng, Sabine Delannoy, David W Lacher, Joseph M Bosilevac, Patrick Fach, Lothar Beutin
Shiga toxin-producing Escherichia coli (STEC) of the O91:H21 serotype has caused severe infections including hemolytic uremic syndrome. Strains of the O91 serogroup have been isolated from food, animals and the environment worldwide, but are not well characterized. We used a microarray and other molecular assays to examine 49 O91 strains (environmental, food and clinical) for virulence potential and phylogenetic relationships. Most of the isolates were identified to be strains of O91:H21 and O91:H14 serotype, with a few O91:H10 strains and one O91:H9 strain...
July 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28685874/prediction-of-contrast-induced-acute-kidney-injury-by-early-post-procedural-analysis-of-urinary-biomarkers-and-intra-renal-doppler-flow-indices-in-patients-undergoing-coronary-angiography
#13
Maciej T Wybraniec, Jerzy Chudek, Maria Bożentowicz-Wikarek, Katarzyna Mizia-Stec
BACKGROUND: The study was designed to evaluate the applicability of combined assessment of urinary biomarkers and intra-renal Doppler flow indices for the prediction of contrast-induced acute kidney injury (CI-AKI) after coronary angiography/percutaneous coronary interventions (CA/PCI). METHODS: This prospective observational study covered 95 consecutive patients with coronary artery disease subject to elective or urgent CA/PCI. Doppler intra-renal flow indices were assessed before and 1 h following CA/PCI...
July 6, 2017: Journal of Interventional Cardiology
https://www.readbyqxmd.com/read/28682564/-complement-factor-b-mutation-in-atypical-hemolytic-uremic-syndrome-rare-cause-of-rare-disease
#14
Luca Visconti, Valeria Cernaro, Gianluigi Ardissino, Martina Sgarbanti, Domenico Ferrara, Giuseppe Visconti, Domenico Santoro, Michele Buemi
Hemolytic uremic syndrome (HUS) is a rare disease characterized by microangiopathic hemolysis, platelet consumption and multiple organ failure with predominant renal involvement. In the most of cases (85-90%), it is associated with enteric infection due to Shiga-toxin or verocytotoxin (STEC-VTEC)-producer Escherichia coli. Rarely, in about 10-15% of cases, HUS develops in the presence of a disorder of alternative complement pathway regulation and it is defined atypical (aHUS). We describe the case of a 65-year-old man who came to our attention with a clinical presentation of aHUS and a clinical course characterized by rapidly progressive acute renal failure (ARF), which required renal replacement treatments, and by a stable clinical picture of hematological impairment as a marker of a non-severe and self-limiting form...
April 2017: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/28680031/suspicion-of-myocarditis-in-a-patient-with-mitral-valve-prolapse
#15
Mateusz Polak, Romuald Wojnicz, Jarosław Myszor, Jan Szczogiel, Katarzyna Mizia-Stec
No abstract text is available yet for this article.
June 30, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28669136/left-ventricular-diverticulum-mimicking-cardiac-tumor
#16
Maciej T Wybraniec, Wojciech Wróbel, Jarosław Myszor, Katarzyna Mizia-Stec
Left ventricular (LV) diverticulum represents a rare and frequently asymptomatic congenital anomaly, which is incidentally discovered during routine transthoracic echocardiography. We present a case of a 66-year-old female patient who was admitted to the cardiology department due to incidental finding of a tumor-like mass associated with the posterior mitral leaflet. Preliminary echocardiographic evaluation revealed a solid structure, suspected of mitral annulus calcification or LV malignancy. However, cardiac contrast-enhanced computed tomography confirmed the presence of LV diverticulum, partially filled with calcified thrombus...
July 2, 2017: Echocardiography
https://www.readbyqxmd.com/read/28656639/comparison-of-immunomagnetic-separation-beads-for-detection-of-six-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-in-different-matrices
#17
A L Kraft, D W Lacher, W L Shelver, J S Sherwood, T M Bergholz
Immunomagnetic separation used with culture based methods has been a useful technique in the detection of pathogens. However, previous studies have not answered many of the necessary questions for real world applications. The objective of this study was to assess the efficacy of different immunomagnetic separation (IMS) bead types in recovery of the correct serogroup from a mixture of big six non-O157 Shiga toxin-producing Escherichia coli strains. To determine the impact of different matrices on recovery, samples of sterile phosphate buffered saline (PBS), sterile and non-sterile cattle faeces, ground beef and lettuce were inoculated with 10 CFU per ml mixture of isolates representing the six serogroups...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28643674/microbiological-and-serological-control-of-escherichia-coli-o157-h7-in-kindergarten-staff-in-buenos-aires-city-and-suburban-areas
#18
Romina J Fernández-Brando, María Marta Amaral, Andrés E Ciocchini, Leticia V Bentancor, Jorge A Trelles, Marcelo Da Rocha, Martín Landriel, Mariana Ugarte, Gabriel Briones, Cristina Ibarra, Marina S Palermo
Shiga toxin (Stx)-producing Escherichia coli (STEC) infections are implicated in the development of the life-threatening hemolytic-uremic syndrome (HUS). Despite the magnitude of the social and economic problems caused by HUS, no licensed vaccine or effective therapy is currently available for human use. Prevention of STEC infections continues being the most important measure to reduce HUS incidence. This is especially true for Argentina where HUS incidence among children is extremely high and shows an endemic pattern...
2017: Medicina
https://www.readbyqxmd.com/read/28640075/maximum-voltage-gradient-technique-for-optimization-of-ablation-for-typical-atrial-flutter-with-zero-fluoroscopy-approach
#19
MULTICENTER STUDY
Karol Deutsch, Janusz Śledź, Mariusz Mazij, Bartosz Ludwik, Michał Labus, Dariusz Karbarz, Bernadetta Pasicka, Michał Chrabąszcz, Arkadiusz Śledź, Monika Klank-Szafran, Laura Vitali-Sendoz, Tomasz Kameczura, Jerzy Śpikowski, Piotr Stec, Marek Ujda, Sebastian Stec
Radiofrequency catheter ablation (RFCA) is an established effective method for the treatment of typical cavo-tricuspid isthmus (CTI)-dependent atrial flutter (AFL). The introduction of 3-dimensional electro-anatomic systems enables RFCA without fluoroscopy (No-X-Ray [NXR]). The aim of this study was to evaluate the feasibility and effectiveness of CTI RFCA during implementation of the NXR approach and the maximum voltage-guided (MVG) technique for ablation of AFL.Data were obtained from prospective standardized multicenter ablation registry...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28630857/effectiveness-of-a-commercial-lactic-acid-bacteria-intervention-applied-to-inhibit-shiga-toxin-producing-escherichia-coli-on-refrigerated-vacuum-aged-beef
#20
Katie R Kirsch, Tamra N Tolen, Jessica C Hudson, Alejandro Castillo, Davey Griffin, T Matthew Taylor
Because of their antagonistic activity towards pathogenic and spoilage bacteria, some members of the lactic acid bacteria (LAB) have been evaluated for use as food biopreservatives. The objectives of this study were to assess the antimicrobial utility of a commercial LAB intervention against O157 and non-O157 Shiga-toxigenic E. coli (STEC) on intact beef strip loins during refrigerated vacuum aging and determine intervention efficacy as a function of mode of intervention application. Prerigor strip loins were inoculated with a cocktail (8...
2017: International journal of food science
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