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https://www.readbyqxmd.com/read/28506884/enhancing-effect-of-50%C3%A2-hz-rotating-magnetic-field-on-induction-of-shiga-toxin-converting-lambdoid-prophages
#1
M Struk, B Grygorcewicz, P Nawrotek, A Augustyniak, M Konopacki, M Kordas, R Rakoczy
Studies aimed at investigating factors and mechanism of induction of prophages, a major pathogenesis factor of Shiga toxin-producing Escherichia coli (STEC), are considered important to develop an effective treatment for STEC infections. In this study, we demonstrated the synergistic effect of the rotating magnetic field (RMF) of induction B = 34 mT and frequency ƒ = 50 Hz at a constant temperature of 37 °C and mitomycin C (MMC), that resulted in a higher level of induction of stx-carrying lambdoid Stx prophages...
May 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28503491/variation-in-the-distribution-of-putative-virulence-and-colonization-factors-in-shiga-toxin-producing-escherichia-coli-isolated-from-different-categories-of-cattle
#2
María E Cáceres, Analía I Etcheverría, Daniel Fernández, Edgardo M Rodríguez, Nora L Padola
Shiga toxin-producing Escherichia coli (STEC) are pathogens of significant public health concern. Several studies have confirmed that cattle are the main reservoir of STEC in Argentina and other countries. Although Shiga toxins represent the primary virulence factors of STEC, the adherence and colonization of the gut are also important in the pathogenesis of the bacteria. The aim of this study was to analyze and to compare the presence of putative virulence factors codified in plasmid -katP, espP, subA, stcE- and adhesins involved in colonization of cattle -efa1, iha- in 255 native STEC strains isolated from different categories of cattle from different production systems...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28499121/molecular-characterization-of-o157-h7-o26-h11-and-o103-h2-shiga-toxin-producing-escherichia-coli-isolated-from-dairy-products
#3
T Douëllou, S Delannoy, S Ganet, P Fach, E Loukiadis, M-C Montel, D Sergentet-Thevenot
Pathogenic Shiga toxin-producing E. coli (STEC) are recognized worldwide as environment and foodborne pathogens which can be transmitted by ingestion of ready-to-eat food such as raw milk-derived products. STEC show a prevalence rate in dairy products of 0.9%, yet comparably few outbreaks have been related to dairy products consumption. In this study, we used rt-qPCR to identify the virulence potential of O157, O26 and O103 STEC strains isolated from raw-milk dairy products by analyzing virulence-related gene frequencies and associations with O-island (OI) 44, OI-48, OI-50, OI-57, OI-71 and OI-122...
April 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28495777/complete-annotated-genome-sequences-of-two-shiga-toxin-producing-escherichia-coli-strains-and-one-atypical-enteropathogenic-e-coli-strain-isolated-from-naturally-colonized-cattle-of-german-origin
#4
Lutz Geue, Christian Menge, Christian Berens, Stefanie A Barth
Shiga toxin-producing Escherichia coli (STEC) strains are important zoonotic enteric pathogens with the main reservoir in cattle. Here, we present the genomes of two STEC strains and one atypical enteropathogenic E. coli strain from cattle origin, obtained during a longitudinal study in German cattle herds.
May 11, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28494766/filmarray%C3%A2-gi-panel-performance-for-the-diagnosis-of-acute-gastroenteritis-or-hemorragic-diarrhea
#5
Antonio Piralla, Giovanna Lunghi, Gianluigi Ardissino, Alessia Girello, Marta Premoli, Erika Bava, Milena Arghittu, Maria Rosaria Colombo, Alessandra Cognetto, Patrizia Bono, Giulia Campanini, Piero Marone, Fausto Baldanti
BACKGROUND: Acute gastroenteritis is a common cause of morbidity and mortality in humans worldwide. The rapid and specific identification of infectious agents is crucial for correct patient management. However, diagnosis of acute gastroenteritis is usually performed with diagnostic panels that include only a few pathogens. In the present bicentric study, the diagnostic value of FilmArray™ GI panels was assessed in unformed stool samples of patients with acute gastroenteritis and in a series of samples collected from pediatric patients with heamorragic diarrhea...
May 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28491244/diarrheagenic-escherichia-coli-pathotypes-frequency-in-khuzestan-province-of-iran
#6
Atieh Darbandi, Parviz Owlia, Saeid Bouzari, Horieh Saderi
BACKGROUND AND OBJECTIVES: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), diffusely adhering E. coli (DAEC) and shiga toxin producing E. coli (STEC) strains are among the most frequent causative agents in acute diarrhea. The aim of this study was to determine the frequency of DEC pathotypes in Khuzestan province...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491053/hydrophobicity-of-residue-128-of-the-stress-inducible-sigma-factor-rpos-is-critical-for-its-activity
#7
Tadayuki Iwase, Takashi Matsuo, Saiko Nishioka, Akiko Tajima, Yoshimitsu Mizunoe
RpoS is a key stress-inducible sigma factor that regulates stress resistance genes in Escherichia coli, such as the katE gene encoding catalase HPII and the glg genes encoding glycogen synthesis proteins. Monitoring RpoS activity can provide information on the stress sensitivity of E. coli isolates in clinical settings because the RpoS in these isolates is often mutated. In the present study, we found a novel, missense point mutation at RpoS residue 128 in a clinical Shiga toxin-producing E. coli (STEC) isolate...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28487231/antiviral-effect-of-compounds-derived-from-angelica-archangelica-l-on-herpes-simplex-virus-1-and-coxsackievirus-b3-infections
#8
Barbara Rajtar, Krystyna Skalicka-Woźniak, Łukasz Świątek, Agnieszka Stec, Anastazja Boguszewska, Małgorzata Polz-Dacewicz
No abstract text is available yet for this article.
May 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28485631/comparative-effect-of-heat-shock-on-survival-of-o157-h7-and-non-o157-shiga-toxigenic-escherichia-coli-and-salmonella-in-lean-beef-with-or-without-moisture-enhancing-ingredients
#9
Akhila Vasan, Steven C Ingham, Barbara H Ingham
Thermal tolerance of pathogenic bacteria has been shown to increase after exposure to sublethal elevated temperatures, or heat shock. We evaluated the effect of heat shock at 48°C on thermal tolerance (D55°C) of cocktails of O157 and non-O157 Shiga toxigenic Escherichia coli (STEC) and Salmonella in lean ground beef with or without moisture-enhancing ingredients. Beef was moisture enhanced to 110% (w) with a 5% NaCl-2.5% sodium tripolyphosphate (w/w) brine. Meat, with or without added brine, was inoculated (∼10(8) CFU/g) and heat shocked at 48°C for 0, 5, or 30 min, followed by isothermal heating at 55°C...
May 9, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28475359/assessment-of-consumer-exposure-to-salmonella-spp-campylobacter-spp-and-shiga-toxin-producing-escherichia-coli-in-meat-products-at-retail-in-the-city-of-sao-paulo-brazil
#10
Christiane Asturiano Ristori, Ruth Estela Gravato Rowlands, Cecília Geraldes Martins, Maria Luisa Barbosa, Luis Fernando Dos Santos, Miyoko Jakabi, Bernadette Dora Gombossy de Melo Franco
Meat products may be vehicles of bacterial pathogens to humans, and Salmonella spp., Campylobacter spp., and Shiga toxin-producing Escherichia coli (STEC) are the most relevant. The aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of Sao Paulo, Brazil. Salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/32) and chicken legs 37...
May 5, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28472389/carbon-monoxide-releasing-molecules-blunt-placental-ischemia-induced-hypertension
#11
Eric M George, Kathy Cockrell, Marietta Arany, David E Stec, John M Rimoldi, Rama S V Gadepalli, Joey P Granger
BACKGROUND: Preeclampsia is a pregnancy complication which manifests as new-onset hypertension, proteinuria, and a spectrum of other symptoms. While the underlying causes are still a subject of much debate, it is commonly believed that placental ischemia is a central cause. The ischemic placenta secretes factors which are believed to be responsible for the maternal syndrome; most notably the anti-angiogenic protein soluble fms-like tyrosine kinase 1 (sFlt-1). We have reported that induction of the carbon monoxide (CO) producing protein heme oxygenase-1 restored angiogenic imbalance and reduced blood pressure in a rat model of placental ischemia, and that CO blocks hypoxia-induced sFlt-1 production from placental tissue in vitro...
May 2, 2017: American Journal of Hypertension
https://www.readbyqxmd.com/read/28468281/distinct-expression-of-immunoglobulin-binding-proteins-in-shiga-toxin-producing-escherichia-coli-implicates-high-protein-stability-and-a-characteristic-phenotype
#12
Dennis Rubin, Wenlan Zhang, Helge Karch, Thorsten Kuczius
Several immunoglobulin-binding proteins of Escherichia coli (Eib) have been isolated from both non-pathogenic and pathogenic E. coli strains. Shiga toxin (Stx)-producing E. coli (STEC) contain eibG either as a single gene or in combination with eibC, while other E. coli strains harbour single or multiple eib genes. The Eib proteins bind human immunoglobulins in a non-immune manner and contribute to bacterial chain-like adherence to human epithelial cells. In this study, the EibG expression in several STEC strains was analysed under different environmental conditions...
April 29, 2017: Toxins
https://www.readbyqxmd.com/read/28465160/laparoscopic-intra-abdominal-patent-processus-vaginalis-ligation-in-pediatric-urology-practice
#13
Nima Baradaran, Case M Wood, Olugbemisola O McCoy, Michaella M Prasad, Andrew A Stec
INTRODUCTION AND OBJECTIVE: Inguinal hernias and communicating hydroceles from a patent processus vaginalis (PPV) are common problems in children. This study provides a detailed description of the laparoscopic intra-abdominal patent processus vaginalis ligation (LIPPL) procedure along with its results in pediatric urology patients. METHODS: Prospectively collected data were captured from children (<18 years) who underwent LIPPL from 2012 to 2014. Demographics as well as postoperative characteristics were reviewed and descriptively analyzed...
April 13, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28458656/change-in-the-structure-of-escherichia-coli-population-and-the-pattern-of-virulence-genes-along-a-rural-aquatic-continuum
#14
Fabienne Petit, Olivier Clermont, Sabine Delannoy, Pierre Servais, Michèle Gourmelon, Patrick Fach, Kenny Oberlé, Matthieu Fournier, Erick Denamur, Thierry Berthe
The aim of this study was to investigate the diversity of the Escherichia coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28454739/antibodies-to-shiga-toxins-in-brazilian-cattle
#15
Bruno B Yamamoto, Daniela Luz, Patrícia A E Abreu, Tatiana B Gotti, Silvio A Vasconcellos, Roxane M F Piazza, Denise S P Q Horton
Cattle are considered a reservoir of Shiga toxin-producing Escherichia coli (STEC). There is no information about the presence of antibodies against Shiga toxins in Brazilian bovine serum. Using ELISA, all sera tested showed antibodies against the two main STEC virulence factors; Stx1 and Stx2. Neutralizing antibodies against Stx1 and/or Stx2 were detected in all but one serum. In conclusion, our results indicated that these animals had been exposed to STEC producing both toxins.
April 25, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28452110/antimicrobial-effects-of-quillaja-saponaria-extract-against-escherichia-coli-o157-h7-and-the-emerging-non-o157-shiga-toxin-producing-e-coli
#16
Snigdha Sewlikar, Doris H D'Souza
Natural alternate methods to control the spread of Shiga toxin-producing Escherichia coli (STEC) are important to prevent foodborne outbreaks. Quillaja saponaria aqueous bark extracts (QE), cleared by the U.S. Food and Drug Administration as a natural flavorant, contain bioactive polyphenols, tannins, and tri-terpenoid saponins with anti-inflammatory and antimicrobial activity. The objective of this study was to determine the effects of commercial QE against E. coli O157:H7 and non-O157 strains over 16 h at 37 °C and RT...
May 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28443248/shiga-toxin-subtypes-of-non-o157-escherichia-coli-serogroups-isolated-from-cattle-feces
#17
Pragathi B Shridhar, Chris Siepker, Lance W Noll, Xiaorong Shi, T G Nagaraja, Jianfa Bai
Shiga toxin producing Escherichia coli (STEC) are important foodborne pathogens responsible for human illnesses. Cattle are a major reservoir that harbor the organism in the hindgut and shed in the feces. Shiga toxins (Stx) are the primary virulence factors associated with STEC illnesses. The two antigenically distinct Stx types, Stx1 and Stx2, encoded by stx1 and stx2 genes, share approximately 56% amino acid sequence identity. Genetic variants exist within Stx1 and Stx2 based on differences in amino acid composition and in cytotoxicity...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28439301/prevalence-virulence-potential-and-pulsed-field-gel-electrophoresis-profiling-of-shiga-toxin-producing-escherichia-coli-strains-from-cattle
#18
Hee-Jin Dong, Soomin Lee, Woohyun Kim, Jae-Uk An, Junhyung Kim, Danil Kim, Seongbeom Cho
BACKGROUND: As a primary source of Shiga-toxin-producing Escherichia coli (STEC) infection, cattle are often targeted to develop strategies for reducing STEC contamination. Monitoring the virulence potentials of STEC isolates from cattle is important for tracing contamination sources, managing outbreaks or sporadic cases, and reducing the risks for human infection. This study aimed to investigate the prevalence of STEC in cattle farm samples in South Korea and to assess their virulence potentials...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28434613/the-diversity-of-beef-safety-a-global-reason-to-strengthen-our-current-systems
#19
REVIEW
Mindy M Brashears, Byron D Chaves
The purpose of this paper is to propose a more integrated and more aggressive system approach to food safety rather than focusing on one segment of the industry, or on one approach as described by or constrained by one set of regulations. We focus on the prevalence and control measures for Salmonella and pathogenic Escherichia coli, particularly, Shiga toxin-producing E. coli (STEC) in live cattle on the farm and in the final raw beef product at retail. We describe the antimicrobial and process control strategies most commonly used during slaughter and processing to prevent and reduce the frequency and concentration of these pathogens in the final product, and we propose points along the food chain where more interventions can be applied to ultimately reduce the prevalence of foodborne pathogens associated with beef and beef products, and to protect public health as well the global food supply...
April 5, 2017: Meat Science
https://www.readbyqxmd.com/read/28429754/real-time-detection-of-tsunami-ionospheric-disturbances-with-a-stand-alone-gnss-receiver-a-preliminary-feasibility-demonstration
#20
Giorgio Savastano, Attila Komjathy, Olga Verkhoglyadova, Augusto Mazzoni, Mattia Crespi, Yong Wei, Anthony J Mannucci
It is well known that tsunamis can produce gravity waves that propagate up to the ionosphere generating disturbed electron densities in the E and F regions. These ionospheric disturbances can be studied in detail using ionospheric total electron content (TEC) measurements collected by continuously operating ground-based receivers from the Global Navigation Satellite Systems (GNSS). Here, we present results using a new approach, named VARION (Variometric Approach for Real-Time Ionosphere Observation), and estimate slant TEC (sTEC) variations in a real-time scenario...
April 21, 2017: Scientific Reports
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