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https://www.readbyqxmd.com/read/28074125/shiga-vero-toxin-producing-escherichia-coli-isolated-from-the-hospital-foods-virulence-factors-o-serogroups-and-antimicrobial-resistance-properties
#1
Reza Ranjbar, Mojtaba Masoudimanesh, Farhad Safarpoor Dehkordi, Nematollah Jonaidi-Jafari, Ebrahim Rahimi
BACKGROUND: According to the presence of the weak, diabetic and immunosuppressive patients in hospitals, hospital foods should have a high quality and safety. Cooking a lot of foods higher than daily requirement, storage of cooked foods in an inappropriate condition and presence of nurses and servants in distribution of food to patients are the main reasons caused contamination of hospital foods. Shiga toxigenic Escherichia coli is one of the common cause of food poisoning in hospitals...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28056274/-serotype-identification-and-antibiotic-susceptibility-of-shiga-toxin-producing-escherichia-coli-in-the-weishan-area-in-shandong-province-china
#2
C C Shao, B Hu, Z W Bi, Z Q Kou, M Fang, B L Chen, Z Q Bi
Objective: To determine the serotypes and drug resistance profiles of Shiga toxin-producing Escherichia coli (STEC) in animal stools from the Weishan area in Shandong Province, China. To provide the basis for further study. Methods: Five hundred animal stool samples (from pigs, cattle, sheep, dogs and birds) were collected from the Weishan area and STEC strains were isolated from these samples. Strains were serotyped by a serum agglutination test, and their drug resistance profiles were determined through antimicrobial sensitivity experiments...
January 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/28049493/presence-of-foodborne-pathogens-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-and-methicillin-resistant-staphylococcus-aureus-in-slaughtered-reindeer-in-northern-finland-and-norway
#3
Sauli Laaksonen, Antti Oksanen, Jérôme Julmi, Claudio Zweifel, Maria Fredriksson-Ahomaa, Roger Stephan
BACKGROUND: Various food-producing animals were recognized in recent years as healthy carriers of bacterial pathogens causing human illness. In northern Fennoscandia, the husbandry of semi-domesticated reindeer (Rangifer tarandus tarandus) is a traditional livelihood and meat is the main product. This study determined the presence of selected foodborne pathogens, methicillin-resistant Staphylococcus aureus (MRSA), and extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in healthy semi-domesticated reindeer at slaughter in northern Finland and Norway...
January 3, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28046078/immune-response-in-calves-vaccinated-with-type-three-secretion-system-antigens-and-shiga-toxin-2b-subunit-of-escherichia-coli-o157-h7
#4
Luisina Martorelli, Sergio Garbaccio, Daniel A Vilte, Adriana A Albanese, María P Mejías, Marina S Palermo, Elsa C Mercado, Cristina E Ibarra, Angel A Cataldi
Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group...
2017: PloS One
https://www.readbyqxmd.com/read/28040174/evaluating-the-efficacy-of-beef-slaughter-line-interventions-by-quantifying-the-six-major-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-using-real-time-multiplex-pcr
#5
Kaushi S T Kanankege, Kelly S Anklam, Catherine M Fick, Megan J Kulow, Charles W Kaspar, Barbara H Ingham, Andrew Milkowski, Dörte Döpfer
Six major Shiga toxin producing Escherichia coli (STEC) serogroups: O26, O103, O145, O111, O121, and O45 have been declared as adulterants in federally inspected raw beef in the USA effective June 4th, 2012 in addition to the routinely tested STEC O157: H7. This study tests a real-time multiplex PCR assay and pooling of the samples to optimize the detection and quantification (prevalence and contamination) of six major non-O157 STEC, regardless of possessing Shiga toxins. To demonstrate the practicality, one large-scale slaughter plant (Plant LS) and one small-scale slaughter plant (Plant SS) located in the Mid-Western USA were sampled, in 2011, before the establishment of 2013 USDA laboratory protocols...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28040162/occurrence-and-characterization-of-food-borne-pathogens-isolated-from-fruit-vegetables-and-sprouts-retailed-in-the-czech-republic
#6
Hana Vojkovská, Petra Myšková, Tereza Gelbíčová, Alena Skočková, Ivana Koláčková, Renáta Karpíšková
Food of non-animal origin is a major component of the human diet and has been considered to pose a low risk from the point of view of bacteriological safety. However, an increase in the number of outbreaks of illness caused by such pathogens and linked to the consumption of fresh fruit and vegetables have been reported from around the world recently. Salmonella spp., STEC (Shiga toxin producing Escherichia coli) and Listeria monocytogenes are among the most frequently identified agents. Additionally, the transmission of antibiotic resistant strains including also the methicillin resistant S...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28024150/orthogonal-lipid-sensors-identify-transbilayer-asymmetry-of-plasma-membrane-cholesterol
#7
Shu-Lin Liu, Ren Sheng, Jae Hun Jung, Li Wang, Ewa Stec, Matthew J O'Connor, Seohyoen Song, Rama Kamesh Bikkavilli, Robert A Winn, Daesung Lee, Kwanghee Baek, Kazumitsu Ueda, Irena Levitan, Kwang-Pyo Kim, Wonhwa Cho
Controlled distribution of lipids across various cell membranes is crucial for cell homeostasis and regulation. We developed an imaging method that allows simultaneous in situ quantification of cholesterol in two leaflets of the plasma membrane (PM) using tunable orthogonal cholesterol sensors. Our imaging revealed marked transbilayer asymmetry of PM cholesterol (TAPMC) in various mammalian cells, with the concentration in the inner leaflet (IPM) being ∼12-fold lower than that in the outer leaflet (OPM). The asymmetry was maintained by active transport of cholesterol from IPM to OPM and its chemical retention at OPM...
December 26, 2016: Nature Chemical Biology
https://www.readbyqxmd.com/read/28011696/genotypic-characterization-and-biofilm-formation-of-shiga-toxin-producing-escherichia-coli
#8
C Picozzi, D Antoniani, I Vigentini, R Foschino
Shiga toxin producing Escherichia coli (STEC) are recognized as one of the most dangerous food-borne pathogens. The production of Shiga toxins together with intimin protein are among the main virulence factors. However, the ability to form biofilm can protect bacteria against environmental factors (i.e. desiccation, exposure to UV rays, predation, etc) and sanitization procedures (cleaning, rinsing, chlorination), increasing their survival on food products and in manufacturing plants. Forty-five isolates collected from food and fecal samples were genotyped by Pulsed Field Gel Electrophoresis (PFGE) analysis with XbaI restriction enzyme and investigated by searching for toxins (stx1, stx2) and intimin (eae) genes and serogroup (O157, O26, O145, O111, O103 and O104)...
December 22, 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28010019/internal-medicine-an%C3%A2-indispensable-field-of%C3%A2-medicine
#9
Katarzyna Mizia-Stec
No abstract text is available yet for this article.
December 22, 2016: Polskie Archiwum Medycyny Wewnętrznej
https://www.readbyqxmd.com/read/28003594/first-isolation-of-hybrid-shigatoxigenic-and-enterotoxigenic-escherichia-coli-stec-etec-strain-harboring-stx2-and-elt-genes-in-republic-of-korea
#10
Kyung-Hwan Oh, Eunkyung Shin, Su-Mi Jung, Jinsuk Im, Seung-Hak Cho, Sahyun Hong, Cheon-Kwon Yoo, Gyung Tae Chung
No abstract text is available yet for this article.
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27995343/pre-procedural-renal-resistive-index-accurately-predicts-contrast-induced-acute-kidney-injury-in-patients-with-preserved-renal-function-submitted-to-coronary-angiography
#11
Maciej T Wybraniec, Maria Bożentowicz-Wikarek, Jerzy Chudek, Katarzyna Mizia-Stec
The study aimed to evaluate the clinical utility of ultrasonographic intra-renal blood flow parameters, together with the wide range of different risk factors, for the prediction of contrast-induced acute kidney injury (CI-AKI) in patients with preserved renal function, referred for coronary angiography or percutaneous coronary interventions (CA/PCI). This prospective study covered 95 consecutive patients (69.5% men; median age 65 years) subject to elective or urgent CA/PCI. Data regarding 128 peri-procedural variables were collected...
December 19, 2016: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/27991739/paediatric-hus-cases-related-to-the-consumption-of-raw-milk-sold-by-vending-machines-in-italy-quantitative-risk-assessment-based-on-escherichia-coli-o157-official-controls-over-7%C3%A2-years
#12
F Giacometti, P Bonilauri, S Piva, G Scavia, S Amatiste, D M Bianchi, M N Losio, S Bilei, G Cascone, D Comin, P Daminelli, L Decastelli, G Merialdi, R Mioni, A Peli, A Petruzzelli, F Tonucci, G Liuzzo, A Serraino
A quantitative risk assessment (RA) was developed to estimate haemolytic-uremic syndrome (HUS) cases in paediatric population associated with the consumption of raw milk sold in vending machines in Italy. The historical national evolution of raw milk consumption phenomenon since 2008, when consumer interest started to grow, and after 7 years of marketing adjustment, is outlined. Exposure assessment was based on the official Shiga toxin-producing Escherichia coli O157:H7 (STEC) microbiological records of raw milk samples from vending machines monitored by the regional Veterinary Authorities from 2008 to 2014, microbial growth during storage, consumption frequency of raw milk, serving size, consumption preference and age of consumers...
December 19, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27988533/determinants-and-taxonomic-consequences-of-extreme-egg-shell-variability-in-i-ramazzottius-i-i-subanomalus-i-biserov-1985-tardigrada
#13
Daniel Stec, Witold Morek, Piotr Gąsiorek, Łukasz Kaczmarek, Łukasz Michalczyk
Nearly a half of known eutardigrade species lay ornamented eggs. The ornamentation is thought to provide attachment of the egg to the substrate and protection for the developing embryo, but from the taxonomic point of view chorion morphology may also provide key characters for species differentiation and identification, especially between closely related taxa. Nonetheless, despite the evolutionary and taxonomic importance of the egg shell, the determinants of its morphology are very poorly, if at all, understood...
December 15, 2016: Zootaxa
https://www.readbyqxmd.com/read/27987365/evaluation-of-the-performance-of-the-iq-check-kits-and-the-usda-microbiology-laboratory-guidebook-methods-for-detection-of-shiga-toxin-producing-e-coli-stec-and-stec-and-salmonella-simultaneously-in-ground-beef
#14
Gian Marco Baranzoni, Pina M Fratamico, Federica Boccia, Lori K Bagi, Gwang-Hee Kim, Aniello Anastasio, Tiziana Pepe
AIMS: To evaluate the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top 7 Shiga toxin-producing E. coli (STEC) (O157:H7, O26, O45, O103, O111, O121, and O145) in ground beef and both STEC and Salmonella in co-inoculated samples. METHODS AND RESULTS: Ground beef samples inoculated with ~10 CFU of STEC or both STEC and S. Typhimurium were stored at 4°C for 72 h, followed by screening with the IQ-Check and BAX System kit (MLG) methods that employ different enrichment media...
December 17, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27986670/emerging-public-health-challenges-of-shiga-toxin-producing-escherichia-coli-related-to-changes-in-the-pathogen-the-population-and-the-environment
#15
Mohamed A Karmali
Emerging public health challenges of Shiga toxin-producing Escherichia coli include increases in disease severity or frequency and shifts in risk factors associated with changes, often interconnected, in the pathogen, the population, and the environment. Mitigation requires a "One Health" approach.Emerging public health challenges of Shiga toxin (stx)-producing Escherichia coli (STEC) include the occurrence of more frequent or severe disease and risk factors shifts associated with changes, often interconnected, in the pathogen, the population, and the environment...
October 21, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/27978802/novel-antibacterial-compounds-and-their-drug-targets-successes-and-challenges
#16
Agnieszka A Kaczor, Andrzej Polski, Karolina Sobótka-Polska, Anna Pachuta-Stec, Magdalena Makarska-Bialokoz, Monika Pitucha
Infectious diseases are one of the most important and urgent health problems in the world. According to the World Health Organization (WHO) statistics, infectious and parasitic diseases are a cause of about 16% of all deaths worldwide and over 40% of deaths in Africa. A considerable progress that has been made during last hundred years in the fight against infectious diseases, in particular bacterial infections, can be attributed mainly to three factors: (1) the general improvement of living conditions, in particular sanitation; (2) development of vaccines and (3) development of efficient antibacterial drugs...
December 12, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27969730/tct-383-analysis-of-urinary-biomarkers-of-contrast-induced-acute-kidney-injury-following-coronary-angiography
#17
Maciej Wybraniec, Maria Bożentowicz-Wikarek, Jerzy Chudek, Katarzyna Mizia-Stec
No abstract text is available yet for this article.
November 1, 2016: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/27965648/surveillance-of-diarrheagenic-escherichia-coli-strains-isolated-from-diarrhea-cases-from-children-adults-and-elderly-at-northwest-of-mexico
#18
Adrian Canizalez-Roman, Héctor M Flores-Villaseñor, Edgar Gonzalez-Nuñez, Jorge Velazquez-Roman, Jorge E Vidal, Secundino Muro-Amador, Gerardo Alapizco-Castro, J Alberto Díaz-Quiñonez, Nidia León-Sicairos
Diarrheagenic Escherichia coli (DEC) strains are a main cause of gastrointestinal disease in developing countries. In this study we report the epidemiologic surveillance in a 4-year period (January 2011 to December 2014) of DEC strains causing acute diarrhea throughout the Sinaloa State, Mexico. DEC strains were isolated from outpatients of all ages with acute diarrhea (N = 1,037). Specific DEC pathotypes were identified by PCR-amplification of genes encoding virulence factors. The adhesion phenotype and antibiotic resistance were also investigated...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27964764/investigation-of-a-national-outbreak-of-stec-escherichia-coli-o157-using-online-consumer-panel-control-methods-great-britain-october-2014
#19
C Sinclair, C Jenkins, F Warburton, G K Adak, J P Harris
In October 2014, Public Health England (PHE) identified cases of Shiga toxin-producing Escherichia coli (STEC) serogroup O157 sharing a multiple locus variable-number tandem repeat analysis (MLVA) profile. We conducted a case-control study using multivariable logistic regression to calculate adjusted odds ratios (aOR) and 95% confidence intervals (CI) testing a range of exposures. Cases were defined as laboratory-confirmed STEC O157 with the implicated MLVA profile, were UK residents aged ⩾18 years with symptom onset between 25 September and 30 October 2014, and had no history of travel abroad within 5 days of symptom onset...
December 14, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27958262/role-of-low%C3%A2-dose-dobutamine-echocardiography-in-predicting-response-to-biventricular-pacing-results-from-the%C3%A2-multicenter-viability-in-cardiac-resynchronisation-therapy-viacrt-study
#20
Edyta Płońska-Gościniak, Jarosław D Kasprzak, Tomasz Kukulski, Katarzyna Mizia-Stec, Ewa Nowalany-Kozielska, Zbigniew Gąsior, Krystian Wita, Władysław Sinkiewicz, Hanna Szwed, Piotr Gościniak, Łukasz Chrzanowski
INTRODUCTION    The response to Cardiac Resynchronisation Therapy (CRT) varies significantly, resulting in lack of improvement among the substantial patients proportion.  OBJECTIVES    To identify mechanical dyssynchrony indices with combination of myocardial viability characteristics for predicting long-term response to CRT.  PATIENTS AND METHODS    ViaCRT was a multicentre study coordinated by the Working Group on Echocardiography of  Polish Cardiac Society. 127 patients with heart failure were assessed prospectively...
December 12, 2016: Polskie Archiwum Medycyny Wewnętrznej
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