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https://www.readbyqxmd.com/read/29468143/metagenomic-characterization-of-the-human-intestinal-microbiota-in-fecal-samples-from-stec-infected-patients
#1
Federica Gigliucci, F A Bastiaan von Meijenfeldt, Arnold Knijn, Valeria Michelacci, Gaia Scavia, Fabio Minelli, Bas E Dutilh, Hamideh M Ahmad, Gerwin C Raangs, Alex W Friedrich, John W A Rossen, Stefano Morabito
The human intestinal microbiota is a homeostatic ecosystem with a remarkable impact on human health and the disruption of this equilibrium leads to an increased susceptibility to infection by numerous pathogens. In this study, we used shotgun metagenomic sequencing and two different bioinformatic approaches, based on mapping of the reads onto databases and on the reconstruction of putative draft genomes, to investigate possible changes in the composition of the intestinal microbiota in samples from patients with Shiga Toxin-producing E...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29460001/detection-and-discrimination-of-five-e-coli-pathotypes-using-a-combinatory-sybr%C3%A2-green-qpcr-screening-system
#2
Elodie Barbau-Piednoir, Sarah Denayer, Nadine Botteldoorn, Katelijne Dierick, Sigrid C J De Keersmaecker, Nancy H Roosens
A detection and discrimination system for five Escherichia coli pathotypes, based on a combination of 13 SYBR® Green qPCR, has been developed, i.e., combinatory SYBR® Green qPCR screening system for pathogenic E. coli (CoSYPS Path E. coli). It allows the discrimination on isolates and the screening of potential presence in food of the following pathotypes of E. coli: shigatoxigenic (STEC) (including enterohemorrhagic (EHEC)), enteropathogenic (EPEC), enteroaggregative (EAggEC), enteroaggregative shigatoxigenic (EAggSTEC), and enteroinvasive (EIEC) E...
February 20, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29457626/-transesophageal-echocardiography-in-adults-guidelines-of-the-working-group-on-echocardiography-of-the-polish-cardiac-society
#3
Piotr Lipiec, Janusz Bąk, Wojciech Braksator, Marcin Fijałkowski, Andrzej Gackowski, Zbigniew Gąsior, Jarosław D Kasprzak, Anna Klisiewicz, Mirosław Kowalski, Tomasz Kukulski, Katarzyna Mizia-Stec, Edyta Płońska-Gościniak, Piotr Pruszczyk, Bożena Sobkowicz, Piotr Szymański, Andrzej Szyszka, Olga Trojnarska, Andrzej Wysokiński, Piotr Hoffman
This document presents current Polish guidelines on the clinical use of transesophageal echocardiography, including guidance of percutaneous procedures and intraoperative echocardiography, in adult patients. The authors present recommendations regarding indications and contraindications, staff and equipment requirements, patient preparation and information, examination protocol, reporting and reimbursement.
2018: Kardiologia Polska
https://www.readbyqxmd.com/read/29457625/-transthoracic-echocardiography-in-adults-guidelines-of-the-working-group-on-echocardiography-of-the-polish-cardiac-society
#4
Piotr Lipiec, Janusz Bąk, Wojciech Braksator, Marcin Fijałkowski, Andrzej Gackowski, Zbigniew Gąsior, Jarosław D Kasprzak, Anna Klisiewicz, Mirosław Kowalski, Tomasz Kukulski, Katarzyna Mizia-Stec, Edyta Płońska-Gościniak, Piotr Pruszczyk, Bożena Sobkowicz, Piotr Szymański, Andrzej Szyszka, Olga Trojnarska, Andrzej Wysokiński, Piotr Hoffman
This document presents current Polish guidelines on the clinical use of transthoracic echocardiography, including stress examinations, in adult patients. The examinations with pocket-size imaging devices are also discussed. The authors present recommendations regarding indications and contraindications, staff and equipment requirements, patient preparation and information, examination protocol, reporting and reimbursement.
2018: Kardiologia Polska
https://www.readbyqxmd.com/read/29454639/neutrophil-to-lymphocyte-ratio-platelet-to-lymphocyte-ratio-and-c-reactive-protein-as-new-and-simple-prognostic-factors-in-patients-with-metastatic-renal-cell-cancer-treated-with-tyrosine-kinase-inhibitors-a-systemic-review-and-meta-analysis
#5
Aleksandra Semeniuk-Wojtaś, Arkadiusz Lubas, Rafał Stec, Tomasz Syryło, Stanisław Niemczyk, Cezary Szczylik
BACKGROUND: Inflammation plays a crucial role in cancer development. In this study, we evaluate the prognostic values of systemic inflammation markers such as neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CRP) for the progression-free survival and overall survival in patients with metastatic renal cell carcinoma treated with tyrosine kinase inhibitors. MATERIALS AND METHODS: PubMed and the Cochrane Library databases were searched for published studies on the effect of NLR, PLR, and CRP in patients with metastatic renal cell carcinoma treated with tyrosine kinase inhibitors...
February 2, 2018: Clinical Genitourinary Cancer
https://www.readbyqxmd.com/read/29449403/high-quality-complete-genome-sequences-of-three-bovine-shiga-toxin-producing-escherichia-coli-o177-h-flic-h25-isolates-harboring-virulent-stx2-and-multiple-plasmids
#6
Haiqing Sheng, Mingrui Duan, Samuel S Hunter, Scott A Minnich, Matthew L Settles, Daniel D New, Jennifer R Chase, Matthew W Fagnan, Carolyn J Hovde
Shiga toxin-producing Escherichia coli (STEC) bacteria are zoonotic pathogens. We report here the high-quality complete genome sequences of three STEC O177:H- ( fliC H25 ) strains, SMN152SH1, SMN013SH2, and SMN197SH3. The assembled genomes consisted of one optical map-verified circular chromosome for each strain, plus two plasmids for SMN013SH2 and three plasmids for SMN152SH1 and SMN197SH3, respectively.
February 15, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29446686/survey-of-foodborne-pathogens-aerobic-plate-counts-total-coliform-counts-and-escherichia-coli-counts-in-leafy-greens-sprouts-and-melons-marketed-in-the-united-states
#7
Guodong Zhang, Yi Chen, Lijun Hu, David Melka, Hua Wang, Anna Laasri, Eric W Brown, Errol Strain, Marc Allard, Vincent K Bunning, Mickey Parish, Steven M Musser, Thomas S Hammack
The objective of this research was to assess the microbiological status of leafy greens, sprouts, and melons from U.S. markets. A total of 14,183 samples of leafy greens, 2,652 samples of sprouts, and 3,411 samples of melons were collected throughout the United States from 2009 to 2014. The samples were analyzed for aerobic plate counts, total coliform counts, Escherichia coli counts, and the presence and levels of Salmonella, Shigella, Listeria monocytogenes, and Shiga toxin-producing E. coli (STEC), depending on the year and type of produce...
February 15, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29446673/a-systematic-review-of-fatigue-in-radiology-is-it-a-problem
#8
Nadia Stec, Danielle Arje, Alan R Moody, Elizabeth A Krupinski, Pascal N Tyrrell
OBJECTIVE: The purpose of this study was to review current literature regarding radiologist fatigue. MATERIALS AND METHODS: A literature search was performed using PubMed. Key words and Medical Subject Heading terms were used to generate refined queries with inclusion and exclusion criteria, focusing on fatigue and error. Results were selected according to these criteria: examined radiologist fatigue and radiologic error stemming from fatigue; experimental results measured as accuracy, error, or performance; and peer-reviewed publication...
February 15, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29443761/haemolytic-uremic-syndrome-due-to-infection-with-adenovirus-a-case-report-and-literature-review
#9
Victoria Birlutiu, Rares Mircea Birlutiu
RATIONALE: Haemolytic-uremic syndrome is a rare but serious complication of bacterial and viral infections, which is characterized by the triad of: acute renal failure, microangiopathic haemolytic anemia and thrombocytopenia, sometimes severe, requiring peritoneal dialysis. In Europe, hemolytic-uremic syndrome (HUS) in paediatric pathology is primarily caused by Shiga toxin-producing Escherichia coli (STEC) O157, followed by O26. Beside these etiologies, there are other bacterial and viral infections, and also noninfectious ones that have been associated to lead to HUS as well: in the progression of neoplasia, medication-related, post-transplantation, during pregnancy or associated with the antiphospholipid syndrome, systemic lupus erythematosus or family causes with autosomal dominant or recessive inheritance...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29439390/pathogenicity-islands-distribution-in-non-o157-shiga-toxin-producing-escherichia-coli-stec
#10
Jimena Soledad-Cadona, Ana Victoria Bustamante, Juliana González, Andrea Mariel-Sanso
Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens associated with outbreaks and hemolytic-uremic syndrome. Cattle and meat foods are the main reservoir and infection source, respectively. Pathogenicity islands (PAIs) play an important role in STEC pathogenicity, and non-locus of the enterocyte effacement(LEE) effector ( nle ) genes present on them encode translocated substrates of the type III secretion system. A molecular risk assessment based on the evaluation of the nle content has been used to predict which STEC strains pose a risk to humans...
February 10, 2018: Genes
https://www.readbyqxmd.com/read/29433068/a-simplified-approach-for-evaluating-sustained-slow-pathway-conduction-for-diagnosis-and-treatment-of-atrioventricular-nodal-reentry-tachycardia-in-children-and-adults
#11
Janusz Sledz, Michal Labus, Mariusz Mazij, Monika Klank-Szafran, Dariusz Karbarz, Bartosz Ludwik, Jacek Kusa, Karol Deutsch, Leslaw Szydlowski, Adrian Mscisz, Jerzy Spikowski, Aleksandra Morka, Tomasz Kameczura, Aleksandra Swietoniowska, Sebastian Stec
PURPOSE: During incremental atrial pacing in patients with atrioventricular nodal reentrant tachycardia, the PR interval often exceeds the RR interval (PR > RR) during stable 1:1 AV conduction. However, the PR/RR ratio has never been evaluated in a large group of patients with pacing from the proximal coronary sinus and after isoproterenol challenge. Our study validates new site of pacing and easier method of identification of PR > RR. MATERIAL AND METHODS: A prospective protocol of incremental atrial pacing from the proximal coronary sinus was carried out in 398 patients (AVNRT-228 and control-170)...
February 9, 2018: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29426403/putative-virulence-factors-and-clonal-relationship-of-o174-shiga-toxin-producing-escherichia-coli-isolated-from-human-food-and-animal-sources
#12
Cecilia Cundon, Claudia Carolina Carbonari, Gisela Zolezzi, Marta Rivas, Adriana Bentancor
Shiga toxin-producing Escherichia coli (STEC) is th etiological agent of gastrointestinal diseases as haemorrhagic colitis and haemolytic-uraemic syndrome (HUS). Shiga toxin (Stx) is the main virulence factor. There are two types, Stx1 and Stx2, and several subtypes. Other virulence factors are involved in pathogenesis. While O157:H7 is the predominant serotype, at present non-O157 STEC strains are increasingly recognized as foodborne pathogens worldwide. In Argentina, STEC O174 stands out as an emerging pathogen and is one of the four most prevalent non-O157 STEC serogroups...
February 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29423730/tellurite-resistance-in-shiga-toxin-producing-escherichia-coli
#13
Gentry L Lewis, Quentin R Jorgensen, John D Loy, Rodney A Moxley
Potassium tellurite (K2TeO3) is an effective selective agent for O157:H7 Shiga toxin-producing Escherichia coli (STEC), whereas tellurite resistance in non-O157 STEC is variable with information on O45 minimal. High-level K2TeO3 resistance in STEC is attributable to the ter gene cluster with terD an indicator of the cluster's presence. Polymerase chain reactions for terD and K2TeO3 minimum inhibitory concentration (MIC) determinations in broth cultures were conducted on 70 STEC and 40 non-STEC control organisms...
February 8, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29421358/ability-of-shiga-toxigenic-escherichia-coli-to-survive-within-dry-surface-biofilms-and-transfer-to-fresh-lettuce
#14
Emelia Hornam Adator, Meining Cheng, Rick Holley, Tim McAllister, Claudia Narvaez-Bravo
Biofilms are known to play important roles in bacterial survival and persistence in food-processing environments. This study aimed to determine the ability of the top 7 STEC serotypes to form biofilms on polystyrene (POL) and stainless steel (SS) plates and to quantify their survival and transfer from dry-surface biofilms to lettuce pieces. The ability of 14 STEC strains to form biofilms on these two materials at different exposure times and temperatures was assessed using crystal violet, Congo red and SEM...
January 25, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29420567/outcome-and-clinical-course-of-ehec-o104-infection-in-hospitalized-patients-a-prospective-single-center-study
#15
J P Albersmeier, J P Bremer, W Dammermann, S Lüth, F Hagenmüller, C Rüther, H Otto, A M Nielsen, U Schumacher, S Ullrich
OBJECTIVES: Shiga-toxin producing O157:H7 Entero Haemorrhagic E. coli [STEC/EHEC] are the most common cause of Haemolytic Uraemic Syndrome [HUS] related to infectious haemorrhagic colitis. Nearly all recommendations on long term treatment of EHEC infections refer to this strain. The 2011 outbreak in Northern Europe was the first of this dimension to be caused by the serotype O104:H4. We report on the 3.5 year follow up of 61 patients diagnosed with symptomatic EHEC O104:H4 infection in spring 2011...
2018: PloS One
https://www.readbyqxmd.com/read/29419916/consensus-opinion-on-diagnosis-and-management-of-thrombotic-microangiopathy-in-australia-and-new-zealand
#16
REVIEW
Lucy Fox, Solomon J Cohney, Joshua Y Kausman, Jake Shortt, Peter D Hughes, Erica M Wood, Nicole M Isbel, Theo de Malmanche, Anne Durkan, Pravin Hissaria, Piers Blombery, Thomas D Barbour
Thrombotic microangiopathy (TMA) arises in a variety of clinical circumstances with the potential to cause significant dysfunction of the kidneys, brain, gastrointestinal tract and heart. TMA should be considered in all patients with thrombocytopenia and anaemia, with an immediate request to the haematology laboratory to look for red cell fragments on a blood film. Whilst TMA of any aetiology generally demands prompt treatment, this is especially so in thrombotic thrombocytopenic purpura (TTP) and atypical haemolytic uraemic syndrome (aHUS), where organ failure may be precipitous, irreversible and fatal...
February 8, 2018: Nephrology
https://www.readbyqxmd.com/read/29416183/the-implementation-of-the-strategy-europe-2020-objectives-in-european-union-countries-the-concept-analysis-and-statistical-evaluation
#17
Małgorzata Stec, Mariola Grzebyk
The European Union (EU), striving to create economic dominance on the global market, has prepared a comprehensive development programme, which initially was the Lisbon Strategy and then the Strategy Europe 2020. The attainment of the strategic goals included in the prospective development programmes shall transform the EU into the most competitive economy in the world based on knowledge. This paper presents a statistical evaluation of progress being made by EU member states in meeting Europe 2020. For the basis of the assessment, the authors proposed a general synthetic measure in dynamic terms, which allows to objectively compare EU member states by 10 major statistical indicators...
2018: Quality & Quantity
https://www.readbyqxmd.com/read/29413210/escherichia-coli-complications-in-pediatric-critical-care
#18
REVIEW
Suzanne S Puentes, Michele Dunstan
Escherichia coli is a bacterium that is an important part of the intestinal tract; however, it has the potential to become pathogenic. Shiga toxin-producing E coli (STEC) is a leading cause of E coli infections and has led to outbreaks in North America. Transmission is through ingestion of contaminated food sources, and via infected humans and animals. Young children infected with STEC are at high risk for developing hemolytic uremic syndrome (HUS). HUS is a clinical syndrome characterized by macroangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure...
March 2018: Critical Care Nursing Clinics of North America
https://www.readbyqxmd.com/read/29412505/labor-and-delivery-nursing-experience-a-prerequisite-for-midwifery-education
#19
Melissa A Stec, Megan W Arbour
No abstract text is available yet for this article.
February 7, 2018: Journal of Midwifery & Women's Health
https://www.readbyqxmd.com/read/29410452/occupational-exposure-to-polycyclic-aromatic-hydrocarbons-and-elevated-cancer-incidence-in-firefighters
#20
Anna A Stec, Kathryn E Dickens, Marielle Salden, Fiona E Hewitt, Damian P Watts, Philip E Houldsworth, Francis L Martin
Cancer incidence appears to be higher amongst firefighters compared to the general population. Given that many cancers have an environmental component, their occupational exposure to products of carbon combustion such as polycyclic aromatic hydrocarbons (PAHs) is of concern. This is the first UK study identifying firefighters exposure to PAH carcinogens. Wipe samples were collected from skin (jaw, neck, hands), personal protective equipment of firefighters, and work environment (offices, fire stations and engines) in two UK Fire and Rescue Service Stations...
February 6, 2018: Scientific Reports
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