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https://www.readbyqxmd.com/read/28317792/incidental-finding-of-a-bubble-shaped-mass-during-coronary-angiography
#1
Tomasz Bochenek, Błażej Kusz, Michał Lelek, Marek Grabka, Katarzyna Mizia-Stec
No abstract text is available yet for this article.
March 20, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28317440/potent-immune-responses-induced-by-a-salmonella-ghost-delivery-system-that-expresses-the-recombinant-stx2eb-fedf-and-feda-proteins-of-the-escherichia-coli-producing-f18-and-shiga-toxin-in-a-murine-model-and-evaluation-of-its-protective-effect-as-a-porcine-vaccine
#2
Gayeon Won, John Hwa Lee
BACKGROUND: In the pathogenicity of porcine edema disease, which is caused by the Escherichia coli-producing F18 and Shiga toxin, F18(+) fimbrial adhesins and Shiga toxin 2e (Stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen. OBJECTIVE: To develop a vaccine candidate against edema disease by combining three selected antigens of F18(+) Shiga toxin-producing Escherichia coli (STEC). METHODS: Genetically engineered Salmonella Typhimurium (ST) ghosts that express Stx2eB, FedF, and FedA were individually inserted in a ghost plasmid cassette (pJHL184), and the resultant plasmids were transformed into a Δasd Δlon ΔcpxR ST (JOL912)...
March 20, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28304216/characterizing-the-multidrug-resistance-of-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-cattle-farms-and-abattoirs
#3
Carrie-Ann Kennedy, Séamus Fanning, Maria Karczmarczyk, Brian Byrne, Áine Monaghan, Declan Bolton, Torres Sweeney
Non-O157 Shiga toxin-producing Escherichia coli (STECs) are not as well characterized as O157 STEC cases, despite their similar prevalence in many countries. Hence, the objective of this study was to investigate the phenotypic and genotypic basis of multidrug resistance (MDR) in non-O157 STEC farm- and abattoir-sourced isolates and assess the potential dissemination of these MDR profiles in vitro. Susceptibility testing to 20 antimicrobials was performed on 146 non-O157 STECs isolated from farm and abattoir environments...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28303131/multiplexed-single-intact-cell-droplet-digital-pcr-music-ddpcr-method-for-specific-detection-of-enterohemorrhagic-e-coli-ehec-in-food-enrichment-cultures
#4
Tanis C McMahon, Burton W Blais, Alex Wong, Catherine D Carrillo
Foodborne illness attributed to enterohemorrhagic E. coli (EHEC), a highly pathogenic subset of Shiga toxin-producing E. coli (STEC), is increasingly recognized as a significant public health issue. Current microbiological methods for identification of EHEC in foods often use PCR-based approaches to screen enrichment broth cultures for characteristic gene markers [i.e., Shiga toxin (stx) and intimin (eae)]. However, false positives arise when complex food matrices, such as beef, contain mixtures of eae-negative STEC and eae-positive E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28300774/protection-of-the-queuosine-biosynthesis-enzyme-quef-from-irreversible-oxidation-by-a-conserved-intramolecular-disulfide
#5
Adeba Mohammad, Adriana Bon Ramos, Bobby W K Lee, Spencer W Cohen, Maryam K Kiani, Dirk Iwata-Reuyl, Boguslaw Stec, Manal A Swairjo
QueF enzymes catalyze the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of the nitrile group of 7-cyano-7-deazaguanine (preQ₀) to 7-aminomethyl-7-deazaguanine (preQ₁) in the biosynthetic pathway to the tRNA modified nucleoside queuosine. The QueF-catalyzed reaction includes formation of a covalent thioimide intermediate with a conserved active site cysteine that is prone to oxidation in vivo. Here, we report the crystal structure of a mutant of Bacillus subtilis QueF, which reveals an unanticipated intramolecular disulfide formed between the catalytic Cys55 and a conserved Cys99 located near the active site...
March 16, 2017: Biomolecules
https://www.readbyqxmd.com/read/28296151/bilirubin-protects-the-aging-heart
#6
EDITORIAL
Samuel O Adeosun, David E Stec
In this issue of Acta Physiologica, Bakrania, et al., examined the effects of hyperbilirubinemia on the aging male heart under basal conditions and after ischemic injury (1) . Heart disease is a significant problem for the aging population in general and males in particular. Ischemic heart disease is a leading cause of global mortality for males and is responsible for one out of four deaths in males in the United States. Therefore, developing novel strategies to combat the development and progression of heart disease has the potential to save millions of lives a year...
March 10, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28294683/evaluation-of-rectoanal-mucosal-swab-sampling-for-molecular-detection-of-enterohemorrhagic-escherichia-coli-in-beef-cattle
#7
Getahun E Agga, Terrance M Arthur, Susanne Hinkley, Joseph M Bosilevac
Cattle are a primary reservoir of enterohemorrhagic Escherichia coli (EHEC), and contaminated beef products are a source of human infections. The U.S. Department of Agriculture Food Safety and Inspection Service declared seven EHEC serogroups (O26, O45, O103, O111, O121, O145, and O157) as adulterants in raw ground beef. Sampling a large number of animals for EHEC surveillance or evaluations of EHEC-focused preharvest interventions requires a convenient and robust sampling method. We evaluated the diagnostic performance of rectoanal mucosal swab (RAMS) for the detection of the top seven EHEC serogroups...
March 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28292660/prevalence-antimicrobial-resistance-and-multiple-locus-variable-number-tandem-repeat-analysis-profiles-of-diarrheagenic-escherichia-coli-isolated-from-different-retail-foods
#8
Lili Wang, Hiromi Nakamura, Eriko Kage-Nakadai, Yukiko Hara-Kudo, Yoshikazu Nishikawa
Diarrheagenic E. coli (DEC) isolates were recovered from local retail markets and the Osaka Municipal Central Wholesale Market in Japan. Retail food samples were collected for analysis in Osaka Japan from 2005 to 2008 and consisted of 32 beef, 28 pork, 20 poultry, 136 fish, 66 fruits and vegetables and 51 ready-to-eat (RTE) food samples. A total of 82 DEC strains were recovered from 64 (19%) food samples with the highest prevalence in poultry (100%, 20/20), followed by pork (54%, 15/28), beef (28%, 9/32), fruits and vegetables (12%, 8/66), fish (6...
March 7, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28287466/sex-dependent-effects-of-ho-1-deletion-from-adipocytes-in-mice
#9
Peter A Hosick, Mary Frances Weeks, Michael W Hankins, Kyle H Moore, David E Stec
Induction of heme oxygenase-1 (HO-1) has been demonstrated to decrease body weight and improve insulin sensitivity in several models of obesity in rodents. To further study the role of HO-1 in adipose tissue, we created an adipose-specific HO-1 knockout mouse model. Male and female mice were fed either a control or a high-fat diet for 30 weeks. Body weights were measured weekly and body composition, fasting blood glucose and insulin levels were determined every six weeks. Adipocyte-specific knockout of HO-1 had no significant effect on body weight in mice fed a high-fat diet but increased body weight in female mice fed a normal-fat diet...
March 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28283424/modelling-plankton-ecosystems-in-the-meta-omics-era-are-we-ready
#10
REVIEW
Krzysztof Franciszek Stec, Luigi Caputi, Pier Luigi Buttigieg, Domenico D'Alelio, Federico Matias Ibarbalz, Matthew B Sullivan, Samuel Chaffron, Chris Bowler, Maurizio Ribera d'Alcalà, Daniele Iudicone
Recent progress in applying meta-omics approaches to the study of marine ecosystems potentially allows scientists to study the genetic and functional diversity of plankton at an unprecedented depth and with enhanced precision. However, while a range of persistent technical issues still need to be resolved, a much greater obstacle currently preventing a complete and integrated view of the marine ecosystem is the absence of a clear conceptual framework. Herein, we discuss the knowledge that has thus far been derived from conceptual and statistical modelling of marine plankton ecosystems, and illustrate the potential power of integrated meta-omics approaches in the field...
March 7, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28281197/wild-birds-and-urban-pigeons-as-reservoirs-for-diarrheagenic-escherichia-coli-with-zoonotic-potential
#11
Clarissa A Borges, Marita V Cardozo, Livia G Beraldo, Elisabete S Oliveira, Renato P Maluta, Kaline B Barboza, Karin Werther, Fernando A Ávila
In order to describe the role of wild birds and pigeons in the transmission of shiga toxigenic Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) to humans and other animals, samples were collected from cloacae and oropharynx of free-living wild birds and free-living pigeons. Two STEC (0.8%) and five EPEC strains (2.0%) were isolated from wild birds and four EPEC strains (2.0%) were recovered from pigeons. Serogroups, sequence types (STs) and virulence genes, such as saa, iha, lpfA O113, ehxA, espA, nleB and nleE, detected in this study had already been implicated in human and animal diseases...
March 9, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28278412/revised-surveillance-case-definitions-shiga-toxin-producing-escherichia-coli-stec
#12
(no author information available yet)
No abstract text is available yet for this article.
September 30, 2016: Communicable Diseases Intelligence Quarterly Report
https://www.readbyqxmd.com/read/28274429/pectic-oligosaccharide-structure-function-relationships-prebiotics-inhibitors-of-escherichia-coli-o157-h7-adhesion-and-reduction-of-shiga-toxin-cytotoxicity-in-ht29-cells
#13
Rong Di, Malathi S Vakkalanka, Chatchaya Onumpai, Hoa K Chau, Andre White, Robert A Rastall, Kit Yam, Arland T Hotchkiss
Shiga toxin (Stx)-producing, food-contaminating Escherichia coli (STEC) is a major health concern. Plant-derived pectin and pectic-oligosaccharides (POS) have been considered as prebiotics and for the protection of humans from Stx. Of five structurally different citrus pectic samples, POS1, POS2 and modified citrus pectin 1 (MCP1) were bifidogenic with similar fermentabilities in human faecal cultures and arabinose-rich POS2 had the greatest prebiotic potential. Pectic oligosaccharides also enhanced lactobacilli growth during mixed batch faecal fermentation...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28272905/g-protein-coupled-receptor-55-gpr55-expresses-differently-in-patients-with-crohn-s-disease-and-ulcerative-colitis
#14
Marcin Włodarczyk, Aleksandra Sobolewska-Włodarczyk, Adam I Cygankiewicz, Damian Jacenik, Wanda M Krajewska, Krystyna Stec-Michalska, Aleksandra Piechota-Polańczyk, Maria Wiśniewska-Jarosińska, Jakub Fichna
AIM: To investigate the levels of G protein-coupled receptor 55 (GPR55) expression in colonic tissue of inflammatory bowel disease (IBD) patients and healthy controls, and its potential implication in IBD treatment. METHODS: Fifty patients were enrolled in our prospective study: n = 21 with Crohn's disease (CD) and n = 16 with ulcerative colitis (UC); 19 women and 18 men. Control consisted of 13 non-IBD patients. In each subject, two biopsies were taken from different colonic locations...
March 8, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28262222/unusual-changes-in-ventricular-repolarization-before-right-ventricular-outflow-tract-arrhythmias
#15
Piotr Kukla, Agnieszka Sławuta, Marek Jastrzębski, Jacek Gajek, Sebastian Stec
No abstract text is available yet for this article.
March 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28260536/nationwide-investigation-of-shiga-toxin-producing-escherichia-coli-among-cattle-in-japan-revealed-the-risk-factors-and-potentially-virulent-subgroups
#16
K Lee, M Kusumoto, T Iwata, S Iyoda, M Akiba
A nationwide study of Shiga toxin-producing Escherichia coli (STEC) was performed to determine the prevalence, characteristics and risk factors for fecal shedding of STEC among cattle in Japan. Information on rearing practices was also collected to identify risk factors for fecal shedding of STEC. STEC was isolated from 24·1% of samples (133/551) collected from 59·1% of farms (65/110). Bayesian clustering using the virulence marker profiles of the isolates subdivided the isolates into four genetically distinct groups, two of which corresponded to eae- or saa-positive STEC, which can cause severe disease in human...
March 6, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28254967/draft-genome-sequence-of-five-shiga-toxin-producing-escherichia-coli-strains-isolated-from-wild-deer-in-japan
#17
Hiroshi Asakura, Tetsuya Ikeda, Shiori Yamamoto, Hidenori Kabeya, Hiromu Sugiyama, Shinji Takai
Shiga toxin-producing Escherichia coli (STEC) is one of the major foodborne pathogens. Having observed the wide distribution of this pathogen in wild deer, we report here the draft genome sequence of five STEC strains isolated from wild deer (Cervus nippon yesoensis) in Hokkaido, Japan.
March 2, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28253564/incorporating-neutrophil-to-lymphocyte-ratio-and-platelet-to-lymphocyte-ratio-in-place-of-neutrophil-count-and-platelet-count-improves-prognostic-accuracy-of-the-international-metastatic-renal-cell-carcinoma-database-consortium-model
#18
Pawel Chrom, Rafal Stec, Lubomir Bodnar, Cezary Szczylik
Purpose: The study investigated whether a replacement of neutrophil count and platelet count by neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) within the International Metastatic Renal Cell Carcinoma Database Consortium (IMDC) model would improve its prognostic accuracy. Materials and Methods: This retrospective analysis included consecutive patients with metastatic renal cell carcinoma treated with first-line tyrosine kinase inhibitors...
March 3, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28235823/an-induced-chromosomal-translocation-in-soybean-disrupts-a-kasi-ortholog-and-is-associated-with-a-high-sucrose-and-low-oil-seed-phenotype
#19
Austin A Dobbels, Jean-Michel Michno, Benjamin W Campbell, Kamaldeep S Virdi, Adrian O Stec, Gary J Muehlbauer, Seth L Naeve, Robert M Stupar
Mutagenesis is a useful tool in many crop species to induce heritable genetic variability for trait improvement and gene discovery. In this study, forward screening of a soybean fast neutron mutant population identified an individual that produced seed with nearly twice the amount of sucrose (8.1% on dry matter basis) and less than half the amount of oil (8.5% on dry matter basis) as compared to wild-type. Bulked segregant analysis, comparative genomic hybridization, and genome resequencing were used to associate the seed composition phenotype with a reciprocal translocation between chromosomes 8 and 13...
February 24, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28225586/multicolor-electrochromic-devices-based-on-molecular-plasmonics
#20
Grant J Stec, Adam Lauchner, Yao Cui, Peter Nordlander, Naomi J Halas
Polycyclic aromatic hydrocarbon (PAH) molecules, the hydrogen-terminated, subnanometer scale version of graphene, support plasmon resonances with the addition or removal of a single electron. Typically colorless when neutral, they are transformed into vivid optical absorbers in either their positively or negatively charged states. Here we demonstrate a low-voltage, multistate electrochromic device based on PAH plasmon resonances that can be reversibly switched between nearly colorless (0 V), olive (+4 V), and royal blue (-3...
February 22, 2017: ACS Nano
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