keyword
MENU ▼
Read by QxMD icon Read
search

Acanthamoeba

keyword
https://www.readbyqxmd.com/read/28811501/characterisation-of-sterol-biosynthesis-and-validation-of-14%C3%AE-demethylase-as-a-drug-target-in-acanthamoeba
#1
Scott Thomson, Christopher A Rice, Tong Zhang, RuAngelie Edrada-Ebel, Fiona L Henriquez, Craig W Roberts
The soil amoebae Acanthamoeba causes Acanthamoeba keratitis, a severe sight-threatening infection of the eye and the almost universally fatal granulomatous amoebic encephalitis. More effective treatments are required. Sterol biosynthesis has been effectively targeted in numerous fungi using azole compounds that inhibit the cytochrome P450 enzyme sterol 14α-demethylase. Herein, using Gas Chromatography Mass Spectrometry (GCMS), we demonstrate that the major sterol of Acanthamoeba castellanii is ergosterol and identify novel putative precursors and intermediate sterols in its production...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807757/strategies-to-counter-transmission-of-superbugs-by-targeting-free-living-amoebae
#2
Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Bacterial infections have remained significant despite our advances in the development of a plethora of disinfectants as well as antimicrobial chemotherapy. This is in part due to our incomplete understanding of the prevalence of bacterial pathogens in the environmental and clinical settings. Several lines of evidence suggest that Acanthamoeba is one of the most ubiquitous/resilient protists that also acts as a host/reservoir for pathogenic microbes. Thus targeting the hardy host, which harbour microbial pathogens, offer a potential avenue to counter infection transmission, particularly hospital/community-acquired infections...
August 11, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28807756/first-time-identification-of-acanthamoeba-genotypes-in-the-cornea-samples-of-wild-birds-is-acanthamoeba-keratitis-making-the-predatory-birds-a-target
#3
Muhammet Karakavuk, Mehmet Aykur, Esra Atalay Şahar, Mehmet Karakuş, Duygu Aldemir, Ömer Döndüren, Hüseyin Gökhan Özdemir, Hüseyin Can, Adnan Yüksel Gürüz, Hande Dağcı, Mert Döşkaya
Acanthamoeba is a free-living amoeba which can be isolated from environment and among others well known as an opportunist protozoan parasite causing infections in humans and animals. Eyes are extremely important for the wild birds and losing sight ability due to Acanthamoeba can be dangerous. The studies on Acanthamoeba infection in wild birds is very few in world and Turkey therefore we aimed to screen deceased wild birds found in İzmir and Manisa provinces located in western Turkey using PCR and non-nutrition agar (NNA) plate method...
August 11, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28797593/osteo-cutaneous-acanthamoebiasis-in-a-non-immunocompromised-patient-with-a-favorable-outcome
#4
Megha Sharma, Shashi Sharma Sudhan, Sonali Sharma, Kirti Megha, Ritambhra Nada, Sumeeta Khurana
Osteo-cutaneous form is a rare presentation of acanthamoebiasis. We present the first such case from India in an apparently healthy male who developed cutaneous lesion with bone involvement after traumatic inoculation of Acanthamoeba cysts. The diagnosis was established by routine microbiological techniques and confirmed by 18SrRNA gene sequencing. Aggressive therapy with terbinafine, chlorhexidine, rifampicin and co-trimoxazole was successful in clearing the lesion and preventing encephalitis.
August 7, 2017: Parasitology International
https://www.readbyqxmd.com/read/28794030/the-investigation-of-promoter-sequences-of-marseilleviruses-highlights-a-remarkable-abundance-of-aaatattt-motif-in-intergenic-regions
#5
Graziele Pereira Oliveira, Maurício Teixeira Lima, Thalita Souza Arantes, Felipe Lopes Assis, Rodrigo Araújo Lima Rodrigues, Flávio Guimarães da Fonseca, Cláudio Antônio Bonjardim, Erna Geessien Kroon, Philippe Colson, Bernard La Scola, Jônatas Santos Abrahão
Viruses display a wide range of genomic profiles and, consequently, a variety of gene expression strategies. Specific sequences associated with transcriptional processes have been described in viruses, and putative promoter motifs have been elucidated for some nucleocytoplasmic large DNA viruses (NCLDV). Among NCLDV, the Marseilleviridae is a well-recognized family because of its genomic mosaicism. The marseilleviruses have an ability to incorporate foreign genes, especially from sympatric organisms inhabiting Acanthamoeba, its main known host...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28763516/crystal-structure-of-mimivirus-uracil-dna-glycosylase
#6
Eunju Kwon, Deepak Pathak, Hyeun Wook Chang, Dong Young Kim
Cytosine deamination induced by stresses or enzymatic catalysis converts deoxycytidine into deoxyuridine, thereby introducing a G to A mutation after DNA replication. Base-excision repair to correct uracil to cytosine is initiated by uracil-DNA glycosylase (UDG), which recognizes and eliminates uracil from DNA. Mimivirus, one of the largest known viruses, also encodes a distinctive UDG gene containing a long N-terminal domain (N-domain; residues 1-130) and a motif-I (residues 327-343), in addition to the canonical catalytic domain of family I UDGs (also called UNGs)...
2017: PloS One
https://www.readbyqxmd.com/read/28761479/surveillance-and-molecular-identification-of-acanthamoeba-and-naegleria-species-in-two-swimming-pools-in-alexandria-university-egypt
#7
Ahmad Z Al-Herrawy, Mahmoud I Khalil, Soheir S El-Sherif, Fatima A E Omar, Wael M Lotfy
BACKGROUND: Swimming in contaminated water was reported to be associated with Acanthamoeba and N. fowleri human infections. The present study was carried out with the aim of isolation and identification of the different species of Acanthamoeba and Naegleria from two swimming pools in Alexandria University. METHODS: Samples were collected from the swimming pools of Alexandria University Stadium and Faculty of Agriculture-Alexandria University during the period from May 2012 to April 2013...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28761472/pathogenic-acanthamoeba-t4-genotype-isolated-from-mucosal-tissue-of-a-patient-with-hiv-infection-a-case-report
#8
Fatemeh Memari, Maryam Niyyati, Zeynab Joneidi
Opportunistic infections due to free-living amoebae such as Granulomatous Amoebic Encephalitis (GAE), cutaneous acanthamoebiasis and disseminated infections could be the causative agent of mortality in people living with HIV/AIDS. In this study, we report the occurrence of the Acanthamoeba belonging to the T4 genotype isolated from nasal and oral swabs of a 15-yr-old man with HIV infection. HIV was confirmed using ELISA kit and RT-PCR assay. The isolated strain showed pathogenic potential using thermo and osmotolerance assays...
January 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28758436/-diagnostics-and-treatment-of-infectious-keratitis
#9
Nóra Szentmáry, László Módis, László Imre, Ágnes Füst, Loay Daas, Lenke Laurik, Berthold Seitz, Zoltán Zsolt Nagy
We summarize up-to-date diagnostic and treatment of infectious keratitis using literature data and some clinical examples. In the clinical practice, most commonly bacterial, herpetic, mycotic and acanthamoeba keratitis occur. Beside slitlamp examination, for diagnostic purpose, we analyse corneal sensitivity, perform in vivo confocal microscopy, polymerase-chain-reaction (PCR), in vitro culture and histological examination of the corneal sample. As conservative treatment we use primarily topical moxifloxacin or cephasolin with fortified tobramycin or gentamycin in bacterial, topical antiviral gel (in some cases in combination with systemic antiviral treatment) in part in combination with topical corticosteroids in herpetic, voriconasole or amphotericin-B in mycotic, and topical-triple-therapy (diamidine, biguanid and antibiotics) in acanthamoeba keratitis...
August 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28757697/corneal-ring-infiltration-in-contact-lens-wearers
#10
Seyed Ali Tabatabaei, Mohammad Soleimani, Mohammadkarim Johari
To report a case of atypical sterile ring infiltrates during wearing soft silicone hydrogel contact lens due to poor lens care. A 29-year-old woman presented with complaints of pain, redness, and morning discharge. She was wearing soft silicone hydrogel contact lens previously; her current symptoms began 1 week before presentation. On examination, best-corrected visual acuity was 20/40 in that eye. Slit-lamp examination revealed dense, ring-shaped infiltrate involving both the superficial and deep stromal layers with lucid interval to the limbus, edema of the epithelium, epithelial defect, and vascularization of the superior limbus...
May 2017: Oman Journal of Ophthalmology
https://www.readbyqxmd.com/read/28756938/isolation-and-molecular-characterization-of-acanthamoeba-from-patients-with-keratitis-in-spain
#11
T Martín-Pérez, A Criado-Fornelio, J Martínez, M A Blanco, I Fuentes, J Pérez-Serrano
In order to improve our knowledge on the epidemiology of amoebic keratitis, as well as the identification of Acanthamoeba isolates, we have isolated Acanthamoeba spp. from five symptomatic patients in Spain in the present study. All isolates were grown in axenic liquid medium, with only one exception. The morphology of these isolates were characterized by optical and scanning electron microscopy. Their structural features corresponded to those of amphizoic amoebae (namely Acanthamoeba spp.). The molecular characterization of the five Acanthamoeba isolates yielded six sequences...
July 4, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/28755026/sensitivity-of-enzymatic-toxins-from-corneal-isolate-of-acanthamoeba-protozoan-to-physicochemical-parameters
#12
Viviane P Sant'Ana, Annette S Foronda, Denise de Freitas, Linda C Carrijo-Carvalho, Fábio Ramos de Souza Carvalho
Acanthamoeba is a free-living amoeba that causes severe corneal infection (Acanthamoeba keratitis) and produces a variety of extracellular enzymes, called exoproteome. Since physicochemical characters are suggested being associated with therapeutic profile and clinical severity of the infection, we investigated the physicochemical properties of proteolysis mediated by amoebic exoproteome. Corneal scraping was collected from a patient who showed typical symptoms of acute Acanthamoeba keratitis. Axenic amoeba was phylogenetically identified by 18S rDNA sequencing analysis...
July 28, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28754142/development-and-validation-of-a-real-time-pcr-assay-for-the-detection-of-clinical-acanthamoebae
#13
Nessika Karsenti, Rachel Lau, Andrew Purssell, Ann Chong-Kit, Marlou Cunanan, Jason Gasgas, Jinfang Tian, Amanda Wang, Filip Ralevski, Andrea K Boggild
BACKGROUND: Suboptimal agreement between molecular assays for the detection of Acanthamoeba spp. in clinical specimens has been demonstrated, and poor assay sensitivity directly imperils the vision of those affected by amoebic keratitis (AK) through delayed diagnosis. We sought to develop and validate a single Taqman real time PCR assay targeting the Acanthamoeba 18S rRNA gene that could be used to enhance sensitivity and specificity when paired with reference assays. METHODS: Biobanked DNA from surplus delinked AK clinical specimens and 10 ATCC strains of Acanthamoeba was extracted...
July 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28719947/efficient-liquid-media-for-encystation-of-pathogenic-free-living-amoebae
#14
Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Suk-Yul Jung, Ho-Joon Shin
Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28709990/in-vitro-development-of-an-effective-treatment-for-acanthamoeba-keratitis
#15
Á Ortillés, J Belloc, E Rubio, M T Fernández, M Benito, J Á Cristóbal, B Calvo, P Goñi
The aim of this study was to develop an in-vitro topical treatment for Acanthamoeba keratitis (AK) effective against cysts and trophozoites. Qualitative assays were performed with voriconazole, chlorhexidine, propamidine, cellulase, tobramycin, ciprofloxacin and paromomycin as monotherapy and various combinations. Riboflavin with ultraviolet-A (R + UV-A) as monotherapy or combined with voriconazole and moxifloxacin was also tested. Quantitative assays to assess cyst viability after treatment were performed for the chemicals that showed the highest activity in the qualitative assays...
July 12, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28708218/microbial-characteristics-of-posttraumatic-infective-keratitis
#16
Blanche X Lim, Victor T C Koh, Manotosh Ray
PURPOSE: To determine the demographics, risk factors, clinical and microbiological characteristics, and treatment outcome of posttraumatic infective keratitis. METHODS: Consecutive patients with posttraumatic infective keratitis presenting to the Ophthalmology Department of a tertiary referral hospital in Singapore between March 2012 and March 2016 were prospectively identified. A standardized data collection form was used to document patient demographics, microbiological diagnosis, antibiotic sensitivity, and pretreatment and posttreatment ocular characteristics...
July 8, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28707344/detection-of-viable-helicobacter-pylori-inside-free-living-amoebae-in-wastewater-and-drinking-water-samples-from-eastern-spain
#17
Laura Moreno-Mesonero, Yolanda Moreno, José Luis Alonso, M Antonia Ferrús
Helicobacter pylori is one of the most concerning emerging waterborne pathogens. It has been suggested that it could survive in water inside free-living amoebae (FLA), but nobody has studied this relationship in the environment yet. Thus, we aimed to detect viable H. pylori cells from inside FLA in water samples. Sixty-nine wastewater and 31 drinking water samples were collected. FLA were purified and identified by PCR and sequencing. For exclusively detecting H. pylori inside FLA, samples were exposed to sodium hypochlorite and assayed by specific PMA-qPCR, DVC-FISH and culture...
July 14, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28704121/evaluation-of-in-vitro-antiamoebic-activity-of-antimicrobial-agents-against-clinical-acanthamoeba-isolates
#18
Hidemasa Nakaminami, Kentaro Tanuma, Kiichi Enomoto, Yukihiko Yoshimura, Tomoyo Onuki, Shin Nihonyanagi, Yukihiro Hamada, Norihisa Noguchi
PURPOSE: The aim of this study was to elucidate in vitro antiamoebic activity of antimicrobial agents at short exposure times similar to those used for actual treatment against Acanthamoeba strains isolated from patients with keratitis. METHODS: The 5 clinical Acanthamoeba isolated in Japan were used in this study. Identification of genotypes for the Acanthamoeba isolates was performed using partial 18S ribosomal DNA (rDNA), including the ASA.S1 region sequences...
July 13, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28700070/granulomatous-amebic-encephalitis-caused-by-acanthamoeba-sp-in-an-immunocompetent-mexican-adult
#19
Elba Guadalupe Rodríguez-Pérez, Kevin Escandón-Vargas, Adriana Ancer
No abstract text is available yet for this article.
May 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28691946/number-of-bacteria-and-time-of-coincubation-with-bacteria-required-for-the-development-of-acanthamoeba-keratitis
#20
Prabhakar Singh, Paavan Kalra, Bhupesh Bagga, Savitri Sharma
No abstract text is available yet for this article.
July 6, 2017: Cornea
keyword
keyword
116438
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"