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https://www.readbyqxmd.com/read/29457249/gut-bacteria-of-cockroaches-are-a-potential-source-of-antibacterial-compound-s
#1
Noor Akbar, Ruqaiyyah Siddiqui, Mazhar Iqbal, K Sagathevan, Naveed Ahmed Khan
Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii...
February 19, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29455336/utilization-of-organic-nitrogen-by-arbuscular-mycorrhizal-fungi-is-there-a-specific-role-for-protists-and-ammonia-oxidizers
#2
Petra Bukovská, Michael Bonkowski, Tereza Konvalinková, Olena Beskid, Martina Hujslová, David Püschel, Veronika Řezáčová, María Semiramis Gutiérrez-Núñez, Milan Gryndler, Jan Jansa
Arbuscular mycorrhizal (AM) fungi can significantly contribute to plant nitrogen (N) uptake from complex organic sources, most likely in concert with activity of soil saprotrophs and other microbes releasing and transforming the N bound in organic forms. Here, we tested whether AM fungus (Rhizophagus irregularis) extraradical hyphal networks showed any preferences towards certain forms of organic N (chitin of fungal or crustacean origin, DNA, clover biomass, or albumin) administered in spatially discrete patches, and how the presence of AM fungal hyphae affected other microbes...
February 17, 2018: Mycorrhiza
https://www.readbyqxmd.com/read/29444861/interacting-heads-motif-has-been-conserved-as-a-mechanism-of-myosin-ii-inhibition-since-before-the-origin-of-animals
#3
Kyoung Hwan Lee, Guidenn Sulbarán, Shixin Yang, Ji Young Mun, Lorenzo Alamo, Antonio Pinto, Osamu Sato, Mitsuo Ikebe, Xiong Liu, Edward D Korn, Floyd Sarsoza, Sanford I Bernstein, Raúl Padrón, Roger Craig
Electron microscope studies have shown that the switched-off state of myosin II in muscle involves intramolecular interaction between the two heads of myosin and between one head and the tail. The interaction, seen in both myosin filaments and isolated molecules, inhibits activity by blocking actin-binding and ATPase sites on myosin. This interacting-heads motif is highly conserved, occurring in invertebrates and vertebrates, in striated, smooth, and nonmuscle myosin IIs, and in myosins regulated by both Ca 2+ binding and regulatory light-chain phosphorylation...
February 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29429293/-deep-anterior-lamellar-keratoplasty-combined-with-antiviral-therapy-in-the-treatment-of-severe-herpes-necrotizing-stromal-keratitis
#4
S X Li, J T Wang, Y Jiang, X Wang, W Y Shi
Objective: To evaluate the efficacy of modified deep lamellar keratoplasty (DLKP) combined with antiviral medications for severe herpes necrotizing stromal keratitis. Methods: Retrospective case series study. Modified DLKP was performed in combination with antiviral medications in fifty patients (50 eyes) with severe necrotizing stromal keratitis, which was unresponsive to systemic and topical antiviral treatment for 1 week, at Shandong Eye Hospital. Before surgery, the operated eyes were examined using slit-lamp microscopy...
February 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29426636/phylogenetic-relationships-among-endosymbiotic-r-body-producer-bacteria-providing-their-host-the-killer-trait
#5
Martina Schrallhammer, Michele Castelli, Giulio Petroni
R-body producing bacterial endosymbionts of Paramecium spp. transform their hosts into "killer" paramecia and provide them a selective advantage. This killer trait is connected to the presence of R-bodies, which are peculiar, tightly coiled protein ribbons capable of rapid unrolling. Based mainly on those two characteristics the respective obligate intracellular bacteria have been comprised in the genus Caedibacter and additional traits such as host species, subcellular localization, and R-body dimensions and mode of unrolling were used for species discrimination...
February 6, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29417274/molecular-evidence-of-chlamydiales-in-ticks-from-wild-and-domestic-hosts-in-sardinia-italy
#6
Valentina Chisu, Cipriano Foxi, Antonio Tanda, Giovanna Masala
Ticks are well known to be important vectors for a wide range of bacteria, viruses and protozoa affecting human and animal health. Ixodid ticks are widely distributed in Sardinia, and an increasing number of tick-borne bacteria have been documented in the island. A growing number of evidence are supporting the hypothesis of alternative transmission routes for chlamydial bacteria such as the involvement of vectors. This study was conducted to provide possible molecular detection of members belonging to the Chlamydiales order in Sardinian ticks and to update information concerning the presence of new ectoparasite-borne bacteria in ticks collected from domestic and wild hosts in a typical Mediterranean environment...
February 7, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29412494/the-rising-tide-of-acanthamoeba-keratitis-in-auckland-new-zealand-a-7-year-review-of-presentation-diagnosis-and-outcomes-2009-2016
#7
James McKelvie, Moaz Alshiakhi, Mohammed Ziaei, Dipika V Patel, Charles Nj McGhee
IMPORTANCE: Acanthamoeba is an increasingly prevalent cause of vision-threatening microbial keratitis. BACKGROUND: To assess the incidence, clinical presentation, diagnosis and outcomes of patients with Acanthamoeba keratitis (AK) in Auckland, New Zealand over a 7-year period. DESIGN: Retrospective observational consecutive case series. PARTICIPANTS: Fifty-eight eyes of 52 patients diagnosed with AK. METHODS: All cases of AK were identified using a cross-referenced search of clinical, laboratory and pharmacy records from March 2009 to May 2016...
February 7, 2018: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/29410444/pro-metastatic-collagen-lysyl-hydroxylase-dimer-assemblies-stabilized-by-fe2-binding
#8
Hou-Fu Guo, Chi-Lin Tsai, Masahiko Terajima, Xiaochao Tan, Priyam Banerjee, Mitchell D Miller, Xin Liu, Jiang Yu, Jovita Byemerwa, Sarah Alvarado, Tamer S Kaoud, Kevin N Dalby, Neus Bota-Rabassedas, Yulong Chen, Mitsuo Yamauchi, John A Tainer, George N Phillips, Jonathan M Kurie
Collagen lysyl hydroxylases (LH1-3) are Fe2+- and 2-oxoglutarate (2-OG)-dependent oxygenases that maintain extracellular matrix homeostasis. High LH2 levels cause stable collagen cross-link accumulations that promote fibrosis and cancer progression. However, developing LH antagonists will require structural insights. Here, we report a 2 Å crystal structure and X-ray scattering on dimer assemblies for the LH domain of L230 in Acanthamoeba polyphaga mimivirus. Loop residues in the double-stranded β-helix core generate a tail-to-tail dimer...
February 6, 2018: Nature Communications
https://www.readbyqxmd.com/read/29405157/an-improvised-medium-for-axenic-cultivation-of-acanthamoeba-spp
#9
Kirti Megha, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium.
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29401523/survival-and-growth-of-stenotrophomonas-maltophilia-in-free-living-amoebae-fla-and-bacterial-virulence-properties
#10
Elodie Denet, Valentin Vasselon, Béatrice Burdin, Sylvie Nazaret, Sabine Favre-Bonté
Stenotrophomonas maltophilia is found ubiquitously in the environment and is an important emerging nosocomial pathogen. S. maltophilia has been recently described as an Amoebae-Resistant Bacteria (ARB) that exists as part of the microbiome of various free-living amoebae (FLA) from waters. Co-culture approaches with Vermamoeba vermiformis demonstrated the ability of this bacterium to resist amoebal digestion. In the present study, we assessed the survival and growth of six environmental and one clinical S. maltophilia strains within two amoebal species: Acanthamoeba castellanii and Willaertia magna...
2018: PloS One
https://www.readbyqxmd.com/read/29398818/an-unusual-cause-of-central-nervous-system-infection-during-acute-myeloid-leukemia-induction-chemotherapy-acanthamoeba-brain-abscess
#11
Ram V Nampoothiri, Pankaj Malhotra, Ankur Jain, Nitya Batra, Kirti Gupta, Fen Saj, Sumeeta Khurana, Harsha Mahalingam, Anupam Lal, Kanchan Mukherjee, Bishan Radotra, Subhash Varma
No abstract text is available yet for this article.
January 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29390396/acute-keratoconjunctivitis-due-to-contamination-of-contact-lens-care-solution-with-histamine-producing-raoultella-species-a-case-report
#12
Hiroshi Eguchi, Fumika Hotta, Tomomi Kuwahara, Haruyuki Nakayama-Imaohji, Shunji Kusaka, Yoshikazu Shimomura
RATIONALE: Contact lens storage cases are known to be contaminated by a significant number of bacteria. However, histamine-producing Raoultella species has not been reported to contaminate contact lens storage case. PATIENT CONCERNS: A 27-year-old woman with keratoconjunctivitis that developed in the left eye owing to a cosmetic contact lens and poor hygiene was referred to our hospital. The corrected visual acuity was hand motion. DIAGNOSES: Corneal infection other than Acanthamoeba keratitis (AK) and corneal hypoxia were excluded...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29378020/multiple-roots-of-fruiting-body-formation-in-amoebozoa
#13
Falk Hillmann, Gillian Forbes, Silvia Novohradská, Iuliia Ferling, Konstantin Riege, Marco Groth, Martin Westermann, Manja Marz, Thomas Spaller, Thomas Winckler, Pauline Schaap, Gernot Glöckner
Establishment of multicellularity represents a major transition in eukaryote evolution. A subgroup of Amoebozoa, the dictyosteliids, has evolved a relatively simple aggregative multicellular stage resulting in a fruiting body supported by a stalk. Protosteloid amoeba, which are scattered throughout the amoebozoan tree, differ by producing only one or few single stalked spores. Thus, one obvious difference in the developmental cycle of protosteliids and dictyosteliids seems to be the establishment of multicellularity...
January 25, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29373390/optimized-protocol-for-testing-multipurpose-contact-lens-solution-efficacy-against-acanthamoeba
#14
Daniel P Fedorko, Jeffrey M Brocious, Katherine D Adams, Victoria M Hitchins, Denise L Hampton, Malvina B Eydelman
OBJECTIVES: To evaluate the interlaboratory and intralaboratory reproducibility of a proposed protocol for multipurpose contact lens solution (MPS) disinfection efficacy against Acanthamoeba. METHODS: Acanthamoeba castellanii and Acanthamoeba polyphaga and four MPS with different biocidal agents were used to evaluate the protocol in two different laboratories. In addition to the negative control, a positive control and neutralization control were used. One experiment was performed in triplicate, and all other experiments were performed in duplicate in each laboratory...
January 24, 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/29359845/acanthamoeba-endophthalmitis-during-treatment-for-cutaneous-disease-in-a-renal-transplant-patient
#15
Allison Kutner, Margaret Aldrich, Sarju Patel, Joann J Kang, Bijal Amin, Ranon Mann, Ibne Karim M Ali, Roosecelis Brasil Martines, Jennifer Rittenhouse Cope, Graciela O De Boccardo, Yoram A Puius
Acanthamoeba infections are difficult to diagnose and treat. We present a renal transplant patient who developed Acanthamoeba endophthalmitis on therapy with posaconazole and miltefosine for cutaneous acanthamobiasis. The patient was maintained on intracameral voriconazole injections, and oral azithromycin, fluconazole, and flucytosine. This case highlights novel presentations and treatments for acanthamoebic infection. This article is protected by copyright. All rights reserved.
January 23, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29357901/can-artificial-tears-prevent-acanthamoeba-keratitis-an-in-vitro-approach
#16
Angela Magnet, Thiago Santos Gomes, Carmen Pardinas, Natalia Garcia de Blas, Cruz Sadaba, Eugenia Carrillo, Fernando Izquierdo, José Manuel Benítez Del Castillo, Carolina Hurtado, Carmen Del Aguila, Soledad Fenoy
BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation...
January 22, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29351066/contamination-of-swimming-pools-and-hot-tubs-biofilms-with-acanthamoeba
#17
Behroz Mahdavi Poor, Abdolhossein Dalimi, Fatemeh Ghafarifar, Fariba Khoshzaban, Jalal Abdolalizadeh
The members of Acanthamoeba genus are ubiquitous amoeba which could be a pathogenic parasite. The amoeba is resistant to the common chlorine concentration that used for disinfecting the swimming pool water. Therefore, the pools can be suitable environments for the survival and multiplication of the amoeba. In this cross sectional study, 10 indoor recreational water centers from different regions of Tabriz city were selected and sampling was done from fixed and floating biofilms of the swimming pools and hot tubs...
March 26, 2018: Acta Parasitologica
https://www.readbyqxmd.com/read/29350163/amebaborne-attilina-massiliensis-keratitis-france
#18
Alexandre Battaini, Bernard La Scola, Gaëlle Ho Wang Yin, Louis Hoffart, Michel Drancourt
We report a case of Acanthamoeba castellani keratitis in a person who wore contact lenses. The amebae hosted an ameba-resistant bacterial symbiont, provisionally named "Attilina massiliensis," a yet undescribed α-Proteobacterium.
February 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29344802/protein-profiling-of-acanthamoeba-species-using-maldi-tof-ms-for-specific-identification-of-acanthamoeba-genotype
#19
Kirti Megha, Megha Sharma, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Acanthamoeba spp. are ubiquitous in the environment and have the potential to cause severe infections. The different genotypes of Acanthamoeba have been shown to influence the severity of the disease and response to therapy. Characterizing Acanthamoeba spp. upto genotype can aid in infection control practices. Twenty-five Acanthamoeba isolates, characterized by 18S rDNA sequencing, were subjected to MALDI-TOF MS analysis by creating a database for the individual genotypes. The differentiating features of the various spectra were observed; the coded samples were then tested against the created database...
January 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29340642/identifying-corneal-infections-in-formalin-fixed-specimens-using-next-generation-sequencing
#20
Zhigang Li, Florian P Breitwieser, Jennifer Lu, Albert S Jun, Laura Asnaghi, Steven L Salzberg, Charles G Eberhart
Purpose: We test the ability of next-generation sequencing, combined with computational analysis, to identify a range of organisms causing infectious keratitis. Methods: This retrospective study evaluated 16 cases of infectious keratitis and four control corneas in formalin-fixed tissues from the pathology laboratory. Infectious cases also were analyzed in the microbiology laboratory using culture, polymerase chain reaction, and direct staining. Classified sequence reads were analyzed with two different metagenomics classification engines, Kraken and Centrifuge, and visualized using the Pavian software tool...
January 1, 2018: Investigative Ophthalmology & Visual Science
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