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Cervical intraepithelial neoplasia

Michela Iacobellis, Cecilia Violante, Gabriella Notarachille, Angela Simone, Rosa Scarfì, Giuseppe Giuffrè
BACKGROUND: According to international guidelines, HPV DNA tests represent a valid alternative to Pap Test for primary cervical cancer screening, provided that they guarantee balanced clinical sensitivity and specificity for cervical intraepithelial neoplasia grade 2 or more severe lesions. The aim of this study was to assess whether REALQUALITY RQ-HPV Screen, a new assay based on real time PCR that targets the E6-E7 region of 14 high-risk human papillomaviruses, meets the criteria for primary cervical cancer screening...
March 20, 2018: Virology Journal
Andrea Ciavattini, Giovanni Delli Carpini, Lorenzo Moriconi, Nicolò Clemente, Nina Montik, Rosa De Vincenzo, Anna Del Fabro, Monica Buttignol, Caterina Ricci, Francesca Moro, Francesco Sopracordevole
OBJECTIVES: To evaluate cervical regeneration at 6 months following excisional treatment for high-grade cervical intraepithelial neoplasia (CIN), and to investigate the effect of cone dimensions, age of patients and technique of excision on the efficacy of the regeneration process. DESIGN: Prospective observational multicentric study. SETTING: Three tertiary care and research centres. PARTICIPANTS: Among the 197 eligible women of childbearing age, older than 25 years of age, undergoing for the first time a loop electrosurgical excision procedure or carbon dioxide laser cervical excision for a high-grade CIN at the colposcopy-directed cervical punch biopsy, and with a final diagnosis of high-grade CIN, 165 completed the 6-month follow-up and were included in the analysis...
March 19, 2018: BMJ Open
Liv Reidun Tverelv, Sveinung Wergeland Sørbye, Finn Egil Skjeldestad
OBJECTIVE: The Norwegian Cervical Cancer Screening Programme recommends follow-up of histologically confirmed normal/cervical intraepithelial neoplasia (CIN) 1 with combined cytology and human papillomavirus testing within 6 to 12 months. This study examines adherence to guidelines and subsequent risk for CIN 3+ within this subset of women. MATERIALS AND METHODS: Women aged 25 to 69 years attending the Norwegian Cervical Cancer Screening Programme in Norway's 2 northernmost counties were included...
March 14, 2018: Journal of Lower Genital Tract Disease
Srividya Budithi, Richard Peevor, David Pugh, Emmanouil Papagiannakis, Amanda Durman, Nassera Banu, Aderemi Alalade, Simon Leeson
BACKGROUND: The diagnostic accuracy of colposcopy is poor for detecting precancerous cervical lesions. OBJECTIVES: We assessed the performance of colposcopy for identifying cervical intraepithelial neoplasia grade 2 or worse (CIN2+), before and after including a dynamic spectral imaging (DSI) map that quantifies and maps acetowhitening to assist subsequent biopsy of suspicious lesions. METHODS: Four hundred and twenty-five women were examined at a multi-center setting in Wales, of which 393 women were included in the final analysis...
March 14, 2018: Gynecologic and Obstetric Investigation
Jieun Son, Yuri Park, Sang-Hyuk Chung
Human papillomavirus (HPV) is required but not sufficient for cervical carcinoma (CxCa). Estradiol (E2 ) promotes CxCa development in K14E7 transgenic mice expressing the HPV16 E7 oncoprotein under the control of the keratin 14 (K14) promoter. E2 mainly works through estrogen receptor α (ERα). However, the role of ERα in human CxCa has been underappreciated largely because it is not expressed in carcinoma cells. We have shown that deletion of Esr1 (the ERα-coding gene) in the cervical stroma of K14E7 mice promotes regression of cervical intraepithelial neoplasia (CIN), the precursor lesion of CxCa...
March 13, 2018: Journal of Pathology
Sugela Blancas, Rogelio Medina-Berlanga, Liliana Ortíz-García, Alfredo Loredo-Ramírez, Leticia Santos
Specific markers in lesions of the human uterine cervix cancer (UCC) are still needed for prognostic, diagnostic and/or therapeutic purposes. In this study we evaluated key molecules at protein level between normal epithelium, cervical intraepithelial neoplasia (CIN1-3) and invasive cancer of a group of molecules previously reported at mRNA level. For that purpose, human formalin-fixed paraffin embedded tissue microarrays (TMAs) were constructed containing 205 Mexican tissue core specimens. Immunohistochemistry and quantitative analysis of histological staining was performed against twenty-two distinct proteins for each core and the processing platform ImageJ...
March 12, 2018: Pathology Oncology Research: POR
Ravit Bassal, Yoav Guilburd, Hagit Shapira, Joseph Azuri, Judith Zandbank, Eduardo Schejter
BACKGROUND: The human papillomavirus (HPV) test has proven to be efficient in triaging women with abnormal Pap findings in women with low cytological atypia, but there is no data about the accuracy for large loop excision of transformation zone in cases of recurrent atypia. OBJECTIVES: To assess the clinical correlation between results of HPV typing and conization histology in women who had recurrent abnormal Pap test results with no colposcopy findings. METHODS: Our retrospective cohort study included 138 women enrolled in the Maccabi Healthcare Services who had consecutive atypical Pap test results for 2 years in which no abnormal colposcopic findings were detected...
March 2018: Israel Medical Association Journal: IMAJ
Nguyen Vu Quoc Huy, Le Minh Tam, Ngo Viet Quynh Tram, Dang Cong Thuan, Truong Quang Vinh, Cao Ngoc Thanh, Linus Chuang
This study aims to determine the diagnostic values of visual inspection with acetic acid (VIA) and Pap smear in a cervical cancer screening program at a community level in Vietnam. A cross-sectional analysis was obtained, including 1034 women of reproductive age from Thua Thien Hue Province, Vietnam from 09/2012 to 09/2013. Samples were taken from cervixes for Pap smear testing, followed by visual inspection with acetic acid. Subjects with abnormal VIA and/or positive cytology results were invited for colposcopy and biopsy...
May 2018: Gynecologic Oncology Reports
Lulu Yu, Mingyue Jiang, Pengpeng Qu, Zeni Wu, Peisong Sun, Mingrong Xi, Yu Qin, Xin Liu, Guangdong Liao, Xiaoqin Lei, Lixin Sun, Yongzhen Zhang, Zhifang Li, Wen Chen, You-Lin Qiao
HPV-16 and -18 account for about 80% of cervical cancers. We evaluated the performance of HPV-16/18 oncoprotein to predict precancer and cancer in corresponding tissue biopsy specimens. 1008 women attending cervical cancer screening program and 638 women referred to colposcopy with biopsy-confirmed cervical intraepithelial neoplasia grade 2 or worse (CIN2+) from 4 hospitals were recruited (1646 in total). All women were tested OncoE6 (AVC), Liquid Based Cytology (Hologic) and cobas HPV test (Roche). Colposcopy was performed on women with any abnormal results...
March 9, 2018: International Journal of Cancer. Journal International du Cancer
Fatma Beyazit, Fatma Sılan, Meryem Gencer, Buket Aydin, Barış Paksoy, Mesut A Unsal, Ozturk Ozdemir
OBJECTIVES: Cervical cancer is the second most common type of cancer for women worldwide with a great proportion proved to be related to human papillomavirus (HPV) infection. As infection with HPV is the strongest risk factor for cervical neoplasia, detection of HPV genotypes in cervical and vaginal specimens of women with normal and abnormal cytology seems to be of paramount importance in cervical cancer screening. The objective of the study is to evaluate the prevalence and HPV genotypes among women with normal or abnormal Pap smear tests...
2018: Ginekologia Polska
Mauricio A Cuello, María E Espinosa, Elisa J Orlandini, Du Y Hwang
OBJECTIVE: To evaluate the usefulness of endocervical curettage (ECC) during loop electrosurgical excision procedures (LEEPs) in predicting the risk of persistence/recurrence of cervical intraepithelial neoplasia grade 2 or higher (CIN2+) and informing clinical decision-making after LEEP. METHODS: The present retrospective study included women undergoing LEEP for CIN2+ at a teaching hospital in Chile between January 1, 2007, and December 31, 2014. Demographic, pathologic, and follow-up data were collected...
March 6, 2018: International Journal of Gynaecology and Obstetrics
Christina Founta, Emmanouil Papagiannakis, Nithya Ratnavelu, Arlene Feusi, Stavros Natsis, Melissa Bradbury, Ann Fisher, Raj Naik
After treatment for cervical intraepithelial neoplasia (CIN), in the UK women who are cytology-negative, high-risk (HR) human papilloma virus (HPV) positive are referred to colposcopy. This pilot study assessed the incidence of residual/recurrent CIN and the diagnostic accuracy of colposcopy with dynamic spectral imaging (DSI) mapping in their detection.This was a prospective service evaluation carried out in a UK National Health Service (NHS) colposcopy clinic. All women, referred with negative cytology/HR-HPV positive result following treatment for CIN from March 2013 until November 2014, who were examined with the DSI digital colposcope were included...
January 2018: Medicine (Baltimore)
H R Wang, Y Lin, X Y Zhang, X T Ma
This study aims to assess the value of transvaginal color Doppler sonography (TVCD) combined with colposcopy for diagnosis of early stage cervical cancer and precancerous lesions. From January 2015 to September 2016, one hundred patients, diagnosed as having early stage cervical cancer and precancerous lesions by histopathological study, were selected as the observation subjects. All patients were examined by TVCD and colposcopy, and the results were compared with histopathological findings. The diagnostic accuracy, sensitivity and specificity of TVCD alone, colposcopy alone, and TVCD combined with colposcopy in early stage of cervical cancer and precancerous lesion were compared...
January 2018: Journal of Biological Regulators and Homeostatic Agents
Ana C O Neves, Camilo L M Morais, Thais P P Mendes, Boniek G Vaz, Kássio M G Lima
Cervical cancer is still an important issue of public health since it is the fourth most frequent type of cancer in women worldwide. Much effort has been dedicated to combating this cancer, in particular by the early detection of cervical pre-cancerous lesions. For this purpose, this paper reports the use of mass spectrometry coupled with multivariate analysis as an untargeted lipidomic approach to classifying 76 blood plasma samples into negative for intraepithelial lesion or malignancy (NILM, n = 42) and squamous intraepithelial lesion (SIL, n = 34)...
March 2, 2018: Scientific Reports
Yingji Jin, Seung Cheol Kim, Hyoung Jin Kim, Woong Ju, Yun Hwan Kim, Hong-Jin Kim
There has been no attempt to apply protein-based markers of exfoliated cervical cells (ECCs) for primary screening of cervical cancer. In the present study, the levels of six tumor-associated protein [TAPs: Sialyl Lewis A (SLeA), Cancer Antigen 15-3 (CA 15-3), p53, heat shock protein (Hsp)70, Hsp27 and squamous cervical carcinoma antigen (SCCA)]and of two human papillomavirus (HPV) viral proteins (HPV16 E7 and HPV16 L1) of ECCs lysates were evaluated by enzyme-linked immunosorbent assays (ELISAs).The wells of 96-well plates were coated with the ECCs lysates from normal, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III and cancer groups, and candidate proteins were detected by relevant antibodies...
February 28, 2018: Archives of Pharmacal Research
Ayodele Gomih, Jennifer S Smith, Kari E North, Michael G Hudgens, Wendy R Brewster, Zhiqing Huang, David Skaar, Fidel Valea, Rex C Bentley, Adriana C Vidal, Rachel L Maguire, Randy L Jirtle, Susan K Murphy, Cathrine Hoyo
The role of host epigenetic mechanisms in the natural history of low-grade cervical intraepithelial neoplasia (CIN1) is not well characterized. We explored differential methylation of imprinted gene regulatory regions as predictors of the risk of CIN1 regression. A total of 164 patients with CIN1 were recruited from 10 Duke University clinics for the CIN Cohort Study. Participants had colposcopies at enrollment and up to five follow-up visits over three years. DNA was extracted from exfoliated cervical cells for methylation quantitation at CpG (cytosine-phosphate-guanine) sites and human papillomavirus (HPV) genotyping...
February 28, 2018: International Journal of Cancer. Journal International du Cancer
Dongmei Tan, Chao Zhou, Sai Han, Xuetao Hou, Shufang Kang, Youzhong Zhang
Cervical cancer is the second most common type of cancer among women worldwide and a leading cause of mortality in women. Metastases reduce the overall survival rate in patients with cervical cancer. Thus, it is clinically urgent to investigate the molecular mechanism of cervical cancer metastasis. The aim of the present study was to investigate the mechanism of microRNA (miR)‑378 in the metastasis of cervical cancer. In the present study, miR‑378 expression levels were significantly upregulated in cervical cancer tissues and cervical intraepithelial neoplasia III tissues when compared with normal cervix tissues...
February 27, 2018: Molecular Medicine Reports
Liming Wang, Hui Shen, Da Zhu, Bei Feng, Lan Yu, Xun Tian, Ci Ren, Chun Gao, Xiaomin Li, Ding Ma, Zheng Hu, Hui Wang
Integration of the high risk human papillomavirus (HR-HPV) genome into host chromatin is an important step in cervical carcinogenesis. We identified HR-HPV integration sites within the human genome through detection of integrated papillomavirus sequences-PCR and assessed the role of high mobility group A 2 (HMGA2) in cervical carcinogenesis in clinical samples and cell lines. HPV integration sites were analyzed in 40 cervical cancer samples, while copy number variation and protein expression were assessed in 19 normal cervixes, 49 cervical intraepithelial neoplasia (CIN), and 52 cervical cancer samples...
January 30, 2018: Oncotarget
Karoliina Tainio, Antonios Athanasiou, Kari A O Tikkinen, Riikka Aaltonen, Jovita Cárdenas, Hernándes, Sivan Glazer-Livson, Maija Jakobsson, Kirsi Joronen, Mari Kiviharju, Karolina Louvanto, Sanna Oksjoki, Riikka Tähtinen, Seppo Virtanen, Pekka Nieminen, Maria Kyrgiou, Ilkka Kalliala
OBJECTIVE: To estimate the regression, persistence, and progression of untreated cervical intraepithelial neoplasia grade 2 (CIN2) lesions managed conservatively as well as compliance with follow-up protocols. DESIGN: Systematic review and meta-analysis. DATA SOURCES: Medline, Embase, and the Cumulative Index to Nursing and Allied Health Literature (CINAHL) from 1 January 1973 to 20 August 2016. ELIGIBILITY CRITERIA: Studies reporting on outcomes of histologically confirmed CIN2 in non-pregnant women, managed conservatively for three or more months...
February 27, 2018: BMJ: British Medical Journal
Yue Zhu, Ying Han, Tian Tian, Peihong Su, Guan Jin, Juan Chen, Yungui Cao
OBJECTIVE: This study aimed to demonstrate the predictive value of miR-21-5p, miR-34a, and human telomerase RNA component (hTERC) in cervical cancer (CC) development and evaluated their potential possibility for future clinical applications. METHODS: Specimens were collected from the normal cervix, cervical intraepithelial neoplasia (CIN) I, CIN II/III, cervical squamous cell carcinoma. Cytological evaluations and histopathologic examinations were conducted in all subjects, along with the assessment of human papillomavirus (HPV) DNA...
January 9, 2018: Pathology, Research and Practice
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