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Cervical intraepithelial neoplasia

J-P Yang, X-J Yang, L Xiao, Y Wang
OBJECTIVE: To investigate long noncoding RNA PVT1 expression in the serum of cervical cancer patients, and to evaluate serum PVT1 level as a diagnostic biomarker for cervical cancer. PATIENTS AND METHODS: Eighty-eight cervical cancer patients, 64 cervical intraepithelial neoplasia patients, 25 breast cancer patients, 25 ovarian cancer patients, and 111 healthy control subjects were enrolled into this study. PVT1 serum level in these participants and PVT1 expression in 20 pairs of cervical cancer tissues and adjacent paired normal tissues was measured by quantitative reverse transcription-polymerase chain reaction...
October 2016: European Review for Medical and Pharmacological Sciences
Peter V Chin-Hong
Human papillomavirus (HPV) is a common infection in kidney transplant recipients. HPV causes cervical, anal, vulvar, vaginal, penile and head and neck cancers. Kidney transplant recipients have a disproportionate burden of disease given prolonged immunosuppression. Given the long pre-invasive state of precancer lesions such as cervical intraepithelial neoplasia (CIN) and anal intraepithelial neoplasia (AIN) most HPV-cancers are preventable with screening and targeted treatment of disease. Pre-transplant vaccination of age-eligible kidney transplant recipients is otherwise ideal...
September 2016: Seminars in Nephrology
Vânia Sammartino Mariano, Adriana Tarlá Lorenzi, Cristovam Scapulatempo-Neto, Maíra Degiovani Stein, Julio Cesar Possati Resende, Márcio Antoniazzi, Luisa Lina Villa, José Eduardo Levi, Adhemar Longatto-Filho, José Humberto Tavares Guerreiro Fregnani
OBJECTIVE: To evaluate the reproducibility and accuracy of the HPV16/18-E6 test. METHODS: The study population was comprised of 448 women with a previously abnormal Pap who were referred to the Barretos Cancer Hospital (Brazil) for diagnosis and treatment. Two cervical samples were collected immediately before colposcopy, one for the hr-HPV-DNA test and cytology and the other for the HPV16/18-E6 test using high-affinity monoclonal antibodies (mAb). Women with a histologic diagnosis of cervical intraepithelial neoplasia grade 2 or 3 were considered to be positive cases...
2016: PloS One
Ying Liu, Chaoting Zhang, Weijiao Gao, Limin Wang, Yaqi Pan, Yunong Gao, Zheming Lu, Yang Ke
HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive 'normal cervical epithelium', 6 HPV-positive CIN2 and 15 HPV-positive CIN 3 samples, respectively. Of the 10 integrations, one and nine were involved with HPV33 and HPV16, respectively. Our study accurately evaluated HPV integration level in CINs and normal cervical tissues using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis...
October 19, 2016: Scientific Reports
Helen A Kelly, Bernard Sawadogo, Admire Chikandiwa, Michel Segondy, Clare Gilham, Olga Lompo, Tanvier Omar, Marie-Noelle Didelot, Nicolas Nagot, Nicolas Meda, Helen A Weiss, Sinead Delany-Moretlwe, Philippe Mayaud
OBJECTIVE: To describe the effect of antiretroviral therapy (ART) and HIV-related factors on high-risk human papillomavirus (HR-HPV) and high-grade cervical intraepithelial neoplasia lesions (CIN2+) among women living with HIV/AIDS (WLHA) in sub-Saharan Africa. DESIGN: Prospective cohort of WLHA in Ouagadougou, Burkina Faso (BF) and Johannesburg, South Africa (SA). Recruitment was stratified by ART status. METHODS: At baseline and endline (median 16 months), cervical samples and biopsies were analyzed for HPV genotyping (InnoLiPA) and by histology...
October 14, 2016: AIDS
Marcelo Simonsen, José Humberto Tavares Guerreiro Fregnani, Júlio Cesar Possati Resende, Márcio Antoniazzi, Adhemar Longatto-Filho, Cristovam Scapulatempo-Neto
OBJECTIVES: To compare the performance of two cervical collection devices (Cytobrush+Ayres spatula and Cervex-Brush® Combi) for cellular sampling, transformation zone representation and accuracy in diagnosing cervical intraepithelial neoplasia (CIN) 2+. METHODS: Cervical samples were collected from patients referred to the colposcopy unit of the Barretos Cancer Hospital between September 2013 and October 2014 using one of the two sampling devices. Additionally, colposcopy was performed with or without cervical biopsy and/or endocervical curettage...
2016: PloS One
Ester Miralpeix, Jordi Genovés, Josep Maria Solé-Sedeño, Gemma Mancebo, Belen Lloveras, Beatriz Bellosillo, Francesc Alameda, Ramon Carreras
p16(INK4a) (p16) tumor-suppressor protein is a biomarker of human papillomavirus (HPV) oncogenic activity that has revealed a high rate of positivity in histological high-gade squamous intraepithelial lesion/cervical intraepithelial neoplasia grade 2 (HSIL/CIN2) lesions. However, there is a paucity of data regarding p16 status as a surrogate marker of HSIL/CIN2 evolution. The aim of this study was to evaluate the outcome of HSIL/CIN2 patients followed up without treatment for 12 months according to p16 immunohistochemical staining...
October 14, 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
L-C Horn, C E Brambs, R Handzel, G Mehlhorn, D Schmidt, K Schierle
The majority of precancerous lesions of the lower female genital tract (intraepithelial neoplasia, IN) are caused by human papillomavirus (HPV) infections resulting in cellular atypia and in turn an altered tissue architecture. Depending on the pathogenesis, a distinction is made between vulvar intraepithelial neoplasia (VIN) classified as classical VIN associated with high-risk HPV infections (u-VIN) and differentiated VIN (d-VIN), which is associated with lichen sclerosus et atrophicus and p53 alterations...
October 13, 2016: Der Pathologe
Elaine W Flagg, Elizabeth A Torrone, Hillard Weinstock
OBJECTIVES: To examine prevalence of low- and high-grade cervical lesions over time in a large cohort of US female adolescents and women. METHODS: We used health care claims data from 9 million privately insured female patients aged 15 to 39 years to estimate annual prevalence of cytologically detected cervical low-grade (LSIL) and high-grade squamous intraepithelial lesions (HSIL) and high-grade histologically detected cervical intraepithelial neoplasia grades 2 and 3 (CIN2+) during 2007 through 2014...
October 13, 2016: American Journal of Public Health
Anneliese Velez-Perez, Xiaohong I Wang, Min Li, Songlin Zhang
Invasive squamous cell carcinoma (SCC) of the cervix involves the progression of premalignant cervical intraepithelial neoplasia (CIN) and is associated with persistent human papilloma virus (HPV) infection. Most CINs will regress, and the challenge is to identify the lesions likely to progress to invasive cancer. We evaluated Sirtuin1 (SIRT1) expression in non-neoplastic cervix, CINs and SCC as a potential biomarker to predict disease progression. A total of 101 cases were selected including 29 CIN 1, 32 CIN 2, 16 CIN 3, 2 microinvasive SCC and 22 invasive SCC...
October 6, 2016: Human Pathology
Hazem Mahmoud Al-Mandeel, Emad Sagr, Khalid Sait, Hassan Mohamed Latifah, Abdulaziz Al-Obaid, Ismail A Al-Badawi, Abdulmohsen O Alkushi, Hany Salem, Nada S Massoudi, Holger Schunemann, Reem A Mustafa, Romina Brignardello-Petersen
BACKGROUND: Cervical cancer is the third most common gynecological malignancy in Saudi women with an estimated incidence rate of 1.9 cases per 100 000 women-years. More than 40% of cervical cancer cases are diagnosed at advanced stages due to lack of a routine screening program in Saudi Arabia. Thus, national guidelines for routine screening and treatment of precancerous cervical lesions are needed. METHODS: The Saudi Centre for Evidence-Based Healthcare invited a panel of local experts and partnered them with a team from McMaster University in Canada for methodological support, to develop national clinical practice guidelines on the screening and treatment of precancerous lesions for cervical cancer...
September 2016: Annals of Saudi Medicine
V G Freitas, G R Focchi, E R Pereira, J E Levi, N M G Speck, J C Ribalta
The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy...
September 16, 2016: Genetics and Molecular Research: GMR
Maaike G Dijkstra, Marjolein van Zummeren, Lawrence Rozendaal, Folkert J van Kemenade, Theo J M Helmerhorst, Peter J F Snijders, Chris J L M Meijer, Johannes Berkhof
OBJECTIVES:  To provide an early risk assessment of extending screening intervals beyond five years for a human papillomavirus (HPV) based cervical screening programme in the Netherlands. DESIGN:  14 year follow-up of a population based randomised cohort from the POBASCAM randomised trial. SETTING:  Organised cervical screening in the Netherlands, based on a programme of three screening rounds (each round done every five years). PARTICIPANTS:  43 339 women aged 29-61 years with a negative HPV and/or negative cytology test participating in the POBASCAM trial...
October 4, 2016: BMJ: British Medical Journal
Shuting Jiang, Yuehong Yang, Min Fang, Xianglang Li, Xiuxue Yuan, Jingping Yuan
Considering the crucial significance of the tumor microenvironment in cancer development and progression, the present study aimed to investigate the changes in macrophages and angiogenesis during the cervical cancer (CC) progression process from chronic cervicitis to cervical intraepithelial neoplasia grades I-III (CIN I-III) to CC. This investigation included quantitative analysis and assessment of the spatial associations between tumor-associated macrophages (TAMs) and tumor neo-vessels. The conventional immunohistochemistry staining technique was used to detect cluster of differentiation (CD)68 and CD105 biomarker expression for TAMs and tumor neo-vessels, respectively...
October 2016: Oncology Letters
Margot M Koeneman, Freyja Hm van Lint, Sander Mj van Kuijk, Luc Jm Smits, Loes Fs Kooreman, Roy Fpm Kruitwagen, Arnold J Kruse
This study aims to develop a prediction model for spontaneous regression of cervical intraepithelial neoplasia grade 2 (CIN 2) lesions based on simple clinical and pathological parameters. The study was conducted at Maastricht University Medical Center, the Netherlands. The prediction model was developed in a retrospective cohort of 129 women with a histological diagnosis of CIN 2 who were managed by watchful waiting for 6-24months. Five potential predictors for spontaneous regression were selected based on the literature and expert opinion and were analyzed in a multivariable logistic regression model, followed by backward stepwise deletion based on the Wald test...
September 30, 2016: Human Pathology
Hong Zhou, Yapei Wang, Qiongying Lv, Juan Zhang, Qing Wang, Fei Gao, Haoli Hou, Hao Zhang, Wei Zhang, Lijia Li
The ribosomal RNA (rRNA) gene encodes rRNA for protein synthesis. Aberrant expression of the rRNA gene has been generally observed in tumor cells and levels of its promoter methylation as an epigenetic regulator affect rRNA gene transcription. The possible relationship between expression and promoter methylation of rDNA has not been examined in human clinical cervical cancer. Here we investigate rRNA gene expression by quantitative real time PCR, and promoter methylation levels by HpaII/MspI digestion and sodium bisulfite sequencing in the development of human cervical cancer...
2016: PloS One
Ahmed Fadiel, Seung Do Choi, Bora Park, Tae-Hee Kim, Julia Buldo-Licciardi, Mitra Ahmadi, Alan Arslan, Khushbakhat Mittal, Frederick Naftolin
RATIONALE: Development of cervical squamous carcinoma (CXCA) is accompanied by changes in estrogen receptors (ERs, ERα and ERβ) and ezrin expression; however, reports have been conflicting. Using histologically documented staging of cervical biopsies, we determined ezrin and ER relationships during CXCA development. METHODS: Immunoreactive (ir) ezrin, ir-ERα, and ir-ERβ were studied in normal epithelium, carcinoma in situ/cervical intraepithelial neoplasia (CIN) 1 to 3, and local invasion or metastatic CXCA...
September 29, 2016: Reproductive Sciences
Vicki B Benard, Philip E Castle, Steven A Jenison, William C Hunt, Jane J Kim, Jack Cuzick, Ji-Hyun Lee, Ruofei Du, Michael Robertson, Scott Norville, Cosette M Wheeler
Importance: A substantial effect of human papillomavirus (HPV) vaccines on reducing HPV-related cervical disease is essential before modifying clinical practice guidelines in partially vaccinated populations. Objective: To determine the population-based cervical intraepithelial neoplasia (CIN) trends when adjusting for changes in cervical screening practices that overlapped with HPV vaccination implementation. Design, Setting, and Participants: The New Mexico HPV Pap Registry, which captures population-based estimates of both cervical screening prevalence and CIN, was used to compute CIN trends from January 1, 2007, to December 31, 2014...
September 29, 2016: JAMA Oncology
J Hong, K A So, J W Kim
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
Nicole J Polman, Margot H Uijterwaal, Birgit I Witte, Johannes Berkhof, Folkert J van Kemenade, Johan W M Spruijt, W Marchien van Baal, Peppino G C M Graziosi, Dorenda K E van Dijken, René H M Verheijen, Theo J M Helmerhorst, Renske D M Steenbergen, Daniëlle A M Heideman, Ruediger Ridder, Peter J F Snijders, Chris J L M Meijer
Women treated for high-grade cervical intraepithelial neoplasia (CIN) are at risk of recurrent CIN Grade 2 or worse (rCIN2+). Currently, posttreatment monitoring is performed using cytology or cytology/high-risk (hr)HPV cotesting. This study aimed to evaluate the performance of p16/Ki-67 dual-stained cytology (p16/Ki-67) for posttreatment monitoring. Three hundred and twenty-three women treated for high-grade CIN in the SIMONATH study underwent close surveillance by cytology, hrHPV and DNA methylation marker testing up to 12 months posttreatment...
September 27, 2016: International Journal of Cancer. Journal International du Cancer
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