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Denise Manica, Cláudia Schweiger, Leo Sekine, Simone Chaves Fagondes, Marisa Gasparin, Deborah Salle Levy, Gabriel Kuhl, Marcus Vinicius Collares, Paulo José Cauduro Marostica
OBJECTIVE: To evaluate the performance of two glossoptosis airway obstruction classifications in predicting symptom severity and laryngeal exposure difficulty in Robin Sequence (RS) patients. SETTING: Public tertiary hospital otolaryngology section (Hospital de Clínicas de Porto Alegre - HCPA). PATIENTS: All RS patients diagnosed at HCPA from October 2012 to February 2015 were enrolled, a total of 58 individuals. They were classified in isolated RS, RS-Plus and syndromic RS...
November 2016: International Journal of Pediatric Otorhinolaryngology
M M de Souza, S C M Niciura, P C Tizioto, A M G Ibelli, G Gasparin, M I P Rocha, F A Bressani, W Malagó-Jr, W J S Diniz, P S N de Oliveira, A O Lima, M A Mudadu, W Barioni Junior, L L Coutinho, L C A Regitano
In contrast to the Mendelian inheritance model, parental alleles can contribute unequally to gene expression, which may result in phenotypic variance among individuals and bias in the predicted additive effect of molecular markers associated with production traits. Given the need to understand the effects of allelic variation and parent-of-origin effects on the expression of genes with a commercial interest in cattle, we analyzed the expression of KCNJ11 (potassium inwardly rectifying channel, subfamily J, member 11), which was previously described as a functional candidate gene for meat tenderness...
August 29, 2016: Genetics and Molecular Research: GMR
Francislaine Aparecida Dos Reis Lívero, Luisa Mota da Silva, Daniele Maria Ferreira, Larissa Favaretto Galuppo, Debora Gasparin Borato, Thiago Bruno Lima Prando, Emerson Luiz Botelho Lourenço, Regiane Lauriano Batista Strapasson, Maria Élida Alves Stefanello, Maria Fernanda de Paula Werner, Alexandra Acco
Ethanol is a psychoactive substance highly consumed around the world whose health problems include gastric lesions. Baccharis trimera is used in folk medicine for the treatment of gastrointestinal disorders. However, few studies have evaluated its biological and toxic effects. To validate the popular use of B. trimera and elucidate its possible antiulcerogenic and cytotoxic mechanisms, a hydroethanolic extract of B. trimera (HEBT) was evaluated in models of gastric lesions. Rats and mice were used to evaluate the protective and antiulcerogenic effects of HEBT on gastric lesions induced by ethanol, acetic acid, and chronic ethanol consumption...
September 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
Marisa Gasparin, Cláudia Schweiger, Denise Manica, Antônio Carlos Maciel, Gabriel Kuhl, Deborah Salle Levy, Paulo José Cauduro Marostica
OBJECTIVE: To investigate the accuracy of clinical evaluation of swallowing in a sample of children with laryngomalacia or glossoptosis and describe the prevalence of dysphagia in each of these diseases, as well as characterize the swallow response to speech and language therapy interventions. STUDY DESIGN: Children aged 1 month to 11 years receiving care at the Department of Otolaryngology, Hospital de Clínicas de Porto Alegre, Brazil, were evaluated in a cross-sectional design...
May 26, 2016: Pediatric Pulmonology
Patrícia Nardin, Caroline Zanotto, Fernanda Hansen, Cristiane Batassini, Manuela Sangalli Gasparin, Patrícia Sesterheim, Carlos-Alberto Gonçalves
Diabetic patients and streptozotocin (STZ)-induced diabetes mellitus (DM) models exhibit signals of brain dysfunction, evidenced by neuronal damage and memory impairment. Astrocytes surrounding capillaries and synapses modulate many brain activities that are connected to neuronal function, such as nutrient flux and glutamatergic neurotransmission. As such, cognitive changes observed in diabetic patients and experimental models could be related to astroglial alterations. Herein, we investigate specific astrocyte changes in the rat hippocampus in a model of DM induced by STZ, particularly looking at glial fibrillary acidic protein (GFAP), S100B protein and glutamate uptake, as well as the content of advanced glycated end products (AGEs) in serum and cerebrospinal fluid (CSF), as a consequence of elevated hyperglycemia and the content of receptor for AGEs in the hippocampus...
August 2016: Neurochemical Research
P C Tizioto, L L Coutinho, G B Mourão, G Gasparin, W Malagó-Jr, F A Bressani, R R Tullio, R T Nassu, J F Taylor, L C A Regitano
The myogenic differentiation 1 gene (MYOD1) has a key role in skeletal muscle differentiation and composition through its regulation of the expression of several muscle-specific genes. We first used a general linear mixed model approach to evaluate the association of MYOD1 expression levels on individual beef tenderness phenotypes. MYOD1mRNA levels measured by quantitative polymerase chain reactions in 136 Nelore steers were significantly associated (P ≤ 0.01) with Warner-Bratzler shear force, measured on the longissimus dorsi muscle after 7 and 14 days of beef aging...
August 2016: Animal Genetics
F S Mesquita, R S Ramos, G Pugliesi, S C S Andrade, V Van Hoeck, A Langbeen, M L Oliveira, A M Gonella-Diaza, G Gasparin, H Fukumasu, L H Pulz, C M Membrive, L L Coutinho, M Binelli
Studying the multitude of molecular networks and pathways that are potentially involved in a complex trait such as fertility requires an equally complex and broad strategy. Here, we used Next-Generation Sequencing for the characterization of the transcriptional signature of the bovine endometrial tissue. Periovulatory endocrine environments were manipulated to generate two distinctly different fertility phenotypes. Cycling, non-lactating, multiparous Nelore cows were manipulated to ovulate larger (> 13 mm; LF group; high fertility phenotype) or smaller (< 12 mm; SF group) follicles...
March 2016: Genomics Data
Caroline Zanotto, Fabrício Simão, Manuela Sangalli Gasparin, Regina Biasibetti, Lucas Silva Tortorelli, Patrícia Nardin, Carlos-Alberto Gonçalves
Diabetes mellitus (DM) is a metabolic disorder associated with micro- and macrovascular alterations that contribute to the cognitive impairment observed in diabetic patients. Signs of breakdown of the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB) have been found in patients and animal models of DM. Breakdown of the BBB and BCSFB can lead to disruptions in cerebral homeostasis and eventually neural dysfunction and degeneration. However, our understanding of the biochemistry underlying barrier protein modifications is incomplete...
March 1, 2016: Molecular Neurobiology
Débora Gasparin Borato, Camila Toledo Scoparo, Daniele Maria-Ferreira, Luísa Mota da Silva, Lauro Mera de Souza, Marcello Iacomini, Maria Fernanda de Paula Werner, Cristiane Hatsuko Baggio
Green tea is an infusion of unfermented leaves of Camellia sinensis (L.) Kuntze (Theaceae), traditionally used for the treatment of obesity, hypercholesterolemia, and gastric complaints. This study evaluated the mechanisms involved in the gastric ulcer healing of the hydroalcoholic extract from green tea (GEt), its ethyl acetate fraction, (GEAc) and epigallocatechin gallate (EGCG) using the model of acetic acid-induced gastric ulcer in rats. The chronic gastric ulcer was induced by application of 80 % acetic acid on serosal mucosa of rats...
March 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
Angela María Gonella-Diaza, Sónia Cristina da Silva Andrade, Mariana Sponchiado, Guilherme Pugliesi, Fernando Silveira Mesquita, Veerle Van Hoeck, Ricardo de Francisco Strefezzi, Gustavo R Gasparin, Luiz L Coutinho, Mario Binelli
In cattle, molecular control of oviduct receptivity to the embryo is poorly understood. Here, we used a bovine model for receptivity based on size of the pre-ovulatory follicle to compare oviductal global and candidate gene transcript abundance on day 4 of the estrous cycle. Growth of the pre-ovulatory follicle (POF) of Nelore (Bos indicus) cows was manipulated to produce two groups: large POF large corpus luteum (CL) group (LF-LCL; greater receptivity) and small POF-small CL group (SF-SCL). Oviductal samples were collected four days after GnRH-induced ovulation...
2015: PloS One
Veerle Van Hoeck, Saara C Scolari, Guilherme Pugliesi, Angela M Gonella-Diaza, Sónia C S Andrade, Gustavo R Gasparin, Luiz L Coutinho, Mario Binelli
The uterus plays a central role among the reproductive tissues in the context of early embryo-maternal communication and a successful pregnancy depends on a complex series of endometrial molecular and cellular events. The factors responsible for the initial interaction between maternal and embryonic tissues, leading to the establishment of pregnancy, remain poorly understood. In this context, Illumina's next-generation sequencing technology has been used to discover the uterine transcriptome signature that is favourable for ongoing pregnancy...
September 2015: Genomics Data
Fernanda Gasparin Palermo, Débora de Paula Soares de Medeiros Albuquerque, Wellington P Martins, Edward Araujo Júnior, Rafael Frederico Bruns
OBJECTIVE: To establish a structured review process to facilitate the identification of the fetal nasal bone (NB) in the first trimester ultrasound scan to improve the quality images. METHODS: We conducted a retrospective observational study in fetal NB images obtained during ultrasound exams of singleton pregnancies that underwent first trimester screening (crown-rump length 45-84 mm). When the images were obtained the examiner was not aware of the study. Audit was conducted by an examiner according criteria established by the Fetal Medicine Foundation...
November 23, 2015: Journal of Maternal-fetal & Neonatal Medicine
Mariella Vieira Pereira Leão, Célia Regina Gonçalves e Silva, Silvana Soléo Ferreira dos Santos, Poliana Gasparin Correa Leite
PURPOSE: To investigate the influence of Lactobacillus rhamnosus in the expression of virulence factors of Candida albicans in vitro. METHODS: A suspension of L. rhamnosus was initially grown in MRS agar. The other day, Sabouraud dextrose agar was added on the growth of lactobacilli and C. albicans was seeded for 24, 48 and 72 hours. Candida strains were then isolated for investigation of the ability of biofilm formation, by means of cultivation into 96 wells plaque, and reading the optical densities and counting colony forming units per mL...
September 2015: Revista Brasileira de Ginecologia e Obstetrícia
A A Gasparin, L Souza, M Siebert, R M Xavier, R M S Chakr, P E Palominos, J C T Brenol, O A Monticielo
OBJECTIVE: The ovarian reserve of patients with systemic lupus erythematosus (SLE) may be affected by disease activity and medication use. Studies have found that patients with SLE have similar fertility rates as healthy women of the same age. The goal of the present study was to investigate the ovarian reserve of patients with SLE by measuring anti-Müllerian hormone (AMH) levels, and compare it to that of healthy controls. METHOD: This was a case-control study performed on 80 premenopausal women, of whom 40 fulfilled the 1997 American College of Rheumatology (ACR) criteria for SLE and 40 healthy controls paired by oral contraceptive use...
March 2016: Lupus
Mariane dos Santos, Rafael N Bringhenti, Patrícia G Rodrigues, Jonathan F do Nascimento, Sane V Pereira, Rafael Zancan, Odirlei A Monticielo, Andrese A Gasparin, Waldir P de Castro, Francisco V Veronese
AIM: Glomerular deposition of immune complexes and inflammation induce podocyte injury in lupus nephritis (LN). This study hypothesized that the severity of the histological lesions of LN affects podocyte-associated mRNAs profiles in kidney tissue and in urine. METHODS: Thirty-three patients with LN were grouped according to the presence of mild mesangial (classes I and II) or moderate-to-severe immune complex deposition, proliferation and/or inflammation (classes III, IV and V) in kidney biopsy...
2015: International Journal of Clinical and Experimental Pathology
F S Mesquita, R S Ramos, G Pugliesi, S C S Andrade, V Van Hoeck, A Langbeen, M L Oliveira, A M Gonella-Diaza, G Gasparin, H Fukumasu, L H Pulz, C M Membrive, L L Coutinho, M Binelli
This study aimed to characterize the endometrial transcriptome and functional pathways overrepresented in the endometrium of cows treated to ovulate larger (≥13 mm) versus smaller (≤12 mm) follicles. Nelore cows were presynchronized prior to receiving cloprostenol (large follicle [LF] group) or not (small follicle [SF] group), along with a progesterone (P4) device on Day (D) -10. Devices were withdrawn and cloprostenol administered 42-60 h (LF) or 30-36 h (SF) before GnRH agonist treatment (D0). Tissues were collected on D4 (experiment [Exp...
August 2015: Biology of Reproduction
Aline S M Cesar, Luciana C A Regitano, James E Koltes, Eric R Fritz-Waters, Dante P D Lanna, Gustavo Gasparin, Gerson B Mourão, Priscila S N Oliveira, James M Reecy, Luiz L Coutinho
Intramuscular fat (IMF) content is related to insulin resistance, which is an important prediction factor for disorders, such as cardiovascular disease, obesity and type 2 diabetes in human. At the same time, it is an economically important trait, which influences the sensorial and nutritional value of meat. The deposition of IMF is influenced by many factors such as sex, age, nutrition, and genetics. In this study Nellore steers (Bos taurus indicus subspecies) were used to better understand the molecular mechanisms involved in IMF content...
2015: PloS One
Karine da Rosa Pereira, Cora Firpo, Marisa Gasparin, Adriane Ribeiro Teixeira, Silvia Dornelles, Tzvi Bacaltchuk, Deborah Salle Levy
Introduction Surgical repair of congenital heart disease in the first years of life compromises the coordination of the suction, breathing, and swallowing functions. Objective To describe the alterations in swallowing found in infants with congenital heart defect during their hospitalization. Methods Prospective, cross-sectional study in a reference hospital for heart disease. The sample consisted of 19 postsurgical patients who underwent an evaluation of swallowing. The infants included were younger than 7 months and had a diagnosis of congenital heart defect and suspected swallowing difficulties...
January 2015: International Archives of Otorhinolaryngology
Mario Binelli, Saara C Scolari, Guilherme Pugliesi, Veerle Van Hoeck, Angela M Gonella-Diaza, Sónia C S Andrade, Gustavo R Gasparin, Luiz L Coutinho
Pregnancy success is critical to the profitability of cattle operations. However, the molecular events driving the uterine tissue towards embryo receptivity are poorly understood. This study aimed to characterize the uterine transcriptome profiles of pregnant (P) versus non-pregnant (NP) cows during early pregnancy and attempted to define a potential set of marker genes that can be valuable for predicting pregnancy outcome. Therefore, beef cows were synchronized (n=51) and artificially inseminated (n=36) at detected estrus...
2015: PloS One
André Rafael Hübner, Daniel Gasparin, Agenor Dias de Meira Junior, Charles Leonardo Israel, Jean Marcel Dambrós, Marcelo Ribeiro, Leandro de Freitas Spinelli
PURPOSE: The research analyses the strength of metallic implants in posterior spinal instrumentation for the treatment of thoracolumbar fractures, considering extended and short fixation techniques on the immediate post-surgical load. Considering that short fixation may bring the advantage of a less invasive surgical procedure to the patient and may also result in lower costs, this evaluation becomes necessary. METHODS: Three-dimensional modelling of the thoracolumbar spine was initially performed...
July 2015: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
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