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Red blood cell antigen

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https://www.readbyqxmd.com/read/29023750/trends-in-antigen-negative-red-blood-cell-distributions-by-racial-or-ethnic-groups-in-the-united-states
#1
Mark H Yazer, Waseem Q Anani, Gregory A Denomme, Matthew S Karafin, Merlyn Sayers, Beth H Shaz
BACKGROUND: The overall number of red blood cell (RBC) units distributed to hospitals throughout the world and in the United States has decreased lately. This study was performed to determine if the number of antigen-negative RBC units distributed to hospitals has followed this trend. STUDY DESIGN AND METHODS: Stratified by ethnicity, data on total RBC distributions and antigen-negative RBC distributions from six large blood collectors in the United States were obtained from 2009 through 2016...
October 11, 2017: Transfusion
https://www.readbyqxmd.com/read/28984339/a-unique-immunofluorescence-protocol-to-detect-protein-expression-in-vascular-tissues-tacking-a-long-standing-pathological-hitch
#2
Puneet Gandhi, Richa Khare
OBJECTIVE: Autofluorescence induced interference is one of the major drawbacks in immunofluorescence analysis of formalin-fixed paraffin-embedded tissues, as it decreases the signal-to-noise ratio of specific labeling. Apart from aldehyde-fixation induced artifacts; collagen and elastin, red blood cells and endogenous fluorescent pigment lipofuscin are prime sources of autofluorescence in vascular and aging tissues. We describe herein, an optimized indirect-immunofluorescence method for archival formalin-fixed paraffin-embedded tissues tissues and cryo sections, using a combination of 3-reagents in a specific order, to achieve optimal fluorescence signals and imaging...
October 6, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28983058/red-cell-alloimmunization-is-associated-with-development-of-autoantibodies-and-increased-red-cell-transfusion-requirements-in-myelodysplastic-syndrome
#3
Deepak Singhal, Monika M Kutyna, Rakchha Chhetri, Li Yan A Wee, Sophia Hague, Lakshmi Nath, Shriram V Nath, Romi Sinha, Nicholas Wickham, Ian D Lewis, David M Ross, Peter G Bardy, Luen Bik To, John Reynolds, Erica M Wood, David J Roxby, Devendra K Hiwase
Up to 90% of myelodysplastic syndrome patients require red blood cell transfusion, however comprehensive data addressing red cell alloimmunization are limited. This study evaluates the incidence and clinical impact of red cell alloimmunization in a large cohort of myelodysplastic syndrome patients registered in the statewide South Australian-MDS registry. Median age of the 817 patients was 73 years, and 66% were male. The cumulative incidence of alloimmunization was 11%. Disease-modifying therapy was associated with a lower risk of alloimmunization...
October 5, 2017: Haematologica
https://www.readbyqxmd.com/read/28982913/perioperative-red-blood-cell-transfusion-is-associated-with-poor-long-term-survival-in-pancreatic-adenocarcinoma
#4
Tomoyuki Abe, Hironobu Amano, Keiji Hanada, Tomoyuki Minami, Shuji Yonehara, Minoru Hattori, Tsuyoshi Kobayashi, Toshikatsu Fukuda, Masahiro Nakahara, Hideki Ohdan, Toshio Noriyuki
BACKGROUND/AIM: Perioperative red blood cell transfusion (RBCT) can negatively affect the host's immune system. We investigated the effects of perioperative RBCT on long-term survival among patients with pancreatic ductal adenocarcinoma (PDAC). PATIENTS AND METHODS: We retrospectively evaluated 148 patients with PDAC who underwent surgery with curative intent (33 who received RBCTs and 115 who did not). Significant prognostic variables on univariate analysis were subjected to multivariate analyses using a Cox proportional hazard regression model...
October 2017: Anticancer Research
https://www.readbyqxmd.com/read/28982373/nanodiamond-enhances-immune-responses-in-mice-against-recombinant-ha-h7n9-protein
#5
Ngoc Bich Pham, Thuong Thi Ho, Giang Thu Nguyen, Thuy Thi Le, Ngoc Thu Le, Huan-Cheng Chang, Minh Dinh Pham, Udo Conrad, Ha Hoang Chu
BACKGROUND: The continuing spread of the newly emerged H7N9 virus among poultry in China, as well as the possibility of human-to-human transmission, has attracted numerous efforts to develop an effective vaccine against H7N9. The use of nanoparticles in vaccinology is inspired by the fact that most pathogens have a dimension within the nano-size range and therefore can be processed efficiently by the immune system, which leads to a potent immune response. Herein, we report a facile approach to increase antigen size to achieve not only fast but also effective responses against the recombinant HA/H7N9 protein via a simple conjugation of the protein onto the surface of nanodiamond particles...
October 5, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28981948/-identification-of-a-bw14-subtype-and-exploration-for-its-molecular-basis
#6
Qing Chen, Ping Li, Jianyu Xiao, Le Lu, Yan Ye, Shuya Wang, Chengyin Huang, Yunlong Zhuang
OBJECTIVE: To identify a rare subtype of the ABO blood group system and explore its molecular basis. METHODS: Based on a standard serological assay, ABO subtype and haplotype were analyzed through PCR amplification of the 7 exons and adjacent introns of the ABO gene and TA clone sequencing. RESULTS: Forward typing showed a B type, while reverse typing demonstrated an extremely weak anti-B on routine gel analysis, which indicated a forward and reverse typing discrepancy...
October 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28981932/-the-role-of-mir-492-in-the-regulation-of-ok-blood-group-antigen-expression-on-red-blood-cells
#7
Luyi Ye, Chen Wang, Qixiu Yang, Ziyan Zhu
OBJECTIVE: To investigate whether miR-492 is involved in the post-transcriptional regulation of OK blood group antigen expression on red blood cells. METHODS: Two 3'-UTR fragments of the BSG gene were synthesized with a chemical method, which respectively encompassed the BSG rs8259 TT or BSG rs8259 AA sites. The fragments were added with Xho I and Not I restriction enzyme cutting sites at both ends and cloned into a pUC57 vector, which in turn was constructed into a psiCHECK-2 vector and verified by sequencing...
October 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28981210/proteome-and-structural-organization-of-the-knob-complex-on-the-surface-of-the-plasmodium-infected-red-blood-cell
#8
ShubaVarshini Alampalli, Manish Grover, Syama Chandran, Utpal Tatu, Pragyan Achary
PURPOSE: The cell membrane of the erythrocytes infected with the malaria parasite Plasmodium falciparum undergoes several changes during the course of parasite life cycle and forms protrusions known as 'knobs' on its surface during the mature trophozoite and schizont stages. The structural organization of knob components especially PfEMP1 on the iRBC surface is the main determinant for the cytoadhesive and rosetting capacity of the iRBC by binding to various host receptors as well as for the variable antigenicity, which is crucial for immunoevasion...
October 5, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28970689/the-prozone-effect-exerted-by-the-complement-binding-anti-le-a-on-anti-d
#9
Sanmukh R Joshi, Kamlesh H Parekh
BACKGROUND: Prozone phenomenon is seen with very high-titer antibodies in an immune serum. AIM: The prozone effect on anti-D by a low-titer anti-Le(a) was investigated associated with neonatal jaundice. MATERIALS AND METHODS: Standard methods were used in investigations. RESULTS: The child was born at full-term developed mild jaundice. With weak direct antiglobulin test+, her indirect serum bilirubin was progressed to 27...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28970680/comparison-of-antibody-titers-using-conventional-tube-technique-versus-column-agglutination-technique-in-abo-blood-group-incompatible-renal-transplant
#10
Amit Bhangale, Amardeep Pathak, Smita Pawar, Tarun Jeloka
INTRODUCTION: Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). MATERIAL AND METHODS: There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the two methods so as to set the 'cut-off' titer accordingly, depending upon the method used...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28970678/inra-a-new-high-frequency-antigen-in-the-indian-in023-blood-group-system
#11
Sanmukh R Joshi, Ankita Sheladiya, Kinjal V Mendapara-Dobariya
BACKGROUND: The INDIAN blood group system comprises 4 antigens sensitive to enzymes and 2-aminoethyl isothiouronium bromide (AET). AIM: The patient's antibody was investigated for its specificity to the high-frequency antigens (HFA) of this system. MATERIAL AND METHODS: Low ionic strength solution (LISS)-tube/LISS-indirect antiglobulin test (IAT) methods were used. The patient's red blood cells (RBCs) were tested with antisera to HFA. Her antibody was tested with RBCs lacking the HFA...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28970677/red-cell-alloimmunization-in-repeatedly-transfused-patients
#12
Dimel K Bhuva, Jitendra H Vachhani
INTRODUCTION: Repeated blood transfusions can result in the production of alloantibodies against one or more red cell antigens, which complicates subsequent transfusions. Aims: The study was done to find incidence of various red cell alloantibodies; to determine the type of alloantibody; to identify the factors such as frequency of transfusion, splenectomy status, donor ethnicity and gender and their association with the development of antibody in repeatedly transfused patients. MATERIALS AND METHODS: This study was carried out in Dept...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28963754/diminished-presentation-of-complement-regulatory-protein-cd55-on-red-blood-cells-from-patients-with-hereditary-haemolytic-anaemias
#13
A Loniewska-Lwowska, K Koza, E Mendek-Czajkowska, P Wieszczy, A Adamowicz-Salach, K Branicka, I Witos, A Sapala-Smoczynska, T Jackowska, J Fabijanska-Mitek
INTRODUCTION: Hereditary haemolytic anaemias (HHA) encompass a heterogeneous group of anaemias characterized by decreased red blood cell survival. The aim of this study was to evaluate the status of red blood cell (RBC) surface molecules known or previously proposed to participate in preventing premature RBC clearance, analysing erythrocytes from patients with two types of HHA: hereditary spherocytosis (HS) and microcytosis. MATERIAL/METHODS: Relative binding of five monoclonal antibodies (mAbs), anti-CD55, anti-CD59, anti-CD44, anti-CD47 and anti-CD58, was evaluated in erythrocytes of patients with HS and hereditary microcytosis, using flow cytometry...
September 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28960464/pde3-inhibitor-attenuates-rat-sinusoidal-obstruction-syndrome-through-inhibition-of-platelet-aggregation-in-disse-s-space
#14
Takashi Miyata, Hidehiro Tajima, Miki Hirata, Shin-Ichi Nakanuma, Isamu Makino, Hironori Hayashi, Katsunobu Oyama, Tomoharu Miyashita, Hiroyuki Takamura, Itasu Ninomiya, Sachio Fushida, Shoichi Iseki, Shin-Ichi Harada, Tomohiko Wakayama, Tetsuo Ohta
BACKGROUND AND AIM: Sinusoidal obstruction syndrome (SOS) is a serious drug-induced liver injury. However, the pathophysiology of the disease remains unclear. This study investigated the effects of cilostazol (CZ), a phosphodiesterase III inhibitor, in a monocrotaline (MCT)-induced rat model of SOS. METHODS: Male Wistar rats were administrated MCT to induce SOS. Rats were divided into control, MCT, and MCT+CZ groups. In the MCT+CZ group, CZ was administered at 48 h, 24 h, and 30 min prior to and 8 h and 24 h after MCT administration...
September 28, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28959352/production-of-erythropoietin-specific-polyclonal-antibodies
#15
Kourosh Maboudi, Seyedeh Marzieh Hosseini, Mina Sepahi, Hashem Yaghoubi, Shahin Hadadian
BACKGROUND: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require specifi c antibodies. High cost, inappropriate shipping (cold chain failures), reduced sensitivity and thus poor detection performance are common pitfalls of using commercial kits for performing immunological tests...
March 2017: Iranian journal of biotechnology
https://www.readbyqxmd.com/read/28938239/absence-of-a-correlation-between-the-abo-blood-group-and-thrombus-burden-in-patients-with-st-segment-elevation-myocardial-infarction
#16
Lutfu Askin, Mustafa Cetin, Serdar Turkmen
OBJECTIVE: ABO antigens are highly abundant in many human cell types, including platelets, vascular endothelium, and red blood cells. The presence of a thrombus is associated with adverse clinical outcomes in patients with ST-segment elevation myocardial infarction (STEMI). We reviewed the relationship between ABO blood groups and thrombus burden in patients with STEMI. PATIENTS AND METHODS: We carried out a retrospective study of 396 patients with STEMI who did not have an ABO blood group or thrombus burden...
September 21, 2017: Coronary Artery Disease
https://www.readbyqxmd.com/read/28930939/preventing-mistransfusions-an-evaluation-of-institutional-knowledge-and-a-response
#17
Nickolas MacDougall, Fanglong Dong, Latoya Broussard, Mark E Comunale
BACKGROUND: Blood product mistransfusions occur when a process error causes transfusion of incompatible blood products. These events are known sources of negative patient outcomes. One such event demonstrated an institutional knowledge gap and an opportunity to reduce this source of transfusion errors. The focus of this study was to evaluate the application of point of care cognitive aids to bridge potentially lethal knowledge gaps in blood product to patient compatibility. METHODS: A patient-donor ABO antigen compatibility grid for red blood cells (RBC) and fresh frozen plasma (FFP) was developed for creation of a cognitive aid and a blood product safety quiz...
September 19, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28919492/human-platelet-antigens-are-associated-with-febrile-non-hemolytic-transfusion-reactions
#18
Ding-Ping Chen, Ying-Hao Wen, Jang-Jih Lu, Ching-Ping Tseng, Wan-Ling Chen, Su-Wei Chang
BACKGROUND: Febrile non-hemolytic transfusion reaction (FNHTR) is the most common type of transfusion reactions, and it could be reduced by transfusing patients with leukocyte-poor blood products. However, FNHTR still occur in certain patients transfused with leukocyte-poor red blood cell (LPR) products. It is examined whether human platelet antigen (HPA) could be a potential membrane antigen that plays a role in FNHTR. METHODS: A total of 120 inpatient subjects who transfused with LPR (60 in FNHTR group, 60 in control group) were typed for HPA-2, HPA-3, and HPA-15 using sequence specific primer-polymerase chain reaction (SSP-PCR) and electrophoresis...
September 14, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28916524/new-methods-to-analyze-b-cell-immune-responses-to-thymus-dependent-antigen-sheep-red-blood-cells
#19
Ellen J McAllister, John R Apgar, Charlotte R Leung, Robert C Rickert, Julia Jellusova
B cells contribute critically to an effective immune response by producing Ag-specific Abs. During the immune response to so-called "thymus-dependent Ags," activated B cells seek T cell help and form germinal centers. In contrast, thymus-independent Ags generally do not induce germinal center formation. In the germinal center, B cells undergo somatic hypermutation, affinity-based clonal expansion, and differentiation to produce plasma cells and memory B cells. Valuable insight into these processes has been gained by using model hapten-carrier complexes or SRBCs...
October 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28905952/detection-of-membrane-bound-and-soluble-antigens-by-magnetic-levitation
#20
Mikkel Schou Andersen, Emily Howard, Shulin Lu, Matthew Richard, Mark Gregory, Gordon Ogembo, Ofer Mazor, Pavel Gorelik, Nathan I Shapiro, Anish V Sharda, Ionita Ghiran
Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field. We describe a novel magnetic levitation-based method that can specifically detect cell membrane-bound and soluble antigens by measurable changes in levitation height that result from the formation of antibody-coated bead and antigen complex. We demonstrate our method's ability to sensitively detect an array of membrane-bound and soluble antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD, red blood cell-bound Epstein-Barr viral particles, and soluble IL-6, and validate the results by flow cytometry and immunofluorescence microscopy performed in parallel...
October 11, 2017: Lab on a Chip
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