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Lipidomics

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https://www.readbyqxmd.com/read/28221327/changes-in-the-lipidomic-profile-of-aqueous-humor-in-open-angle-glaucoma
#1
Javier Cabrerizo, Javier A Urcola, Elena Vecino
PURPOSE: To identify and determine differences in lipid profiles of aqueous humor (AH) in patients with open-angle glaucoma (OAG). MATERIALS AND METHODS: The lipidomic profile of 10 samples of AH of patients with OAG and 10 controls was analyzed. Patients with a history of anterior segment surgery, anterior segment pathology, or intraocular injections were excluded. Topical ocular medications within the last 6 months were reported. AH was obtained during the first step of nonperforating deep sclerotomy in OAG patients and during refractive lensectomy in controls...
February 17, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28220056/a-lipidomic-and-metabolomic-serum-signature-from-nonhuman-primates-exposed-to-ionizing-radiation
#2
Evan L Pannkuk, Evagelia C Laiakis, Tytus D Mak, Giuseppe Astarita, Simon Authier, Karen Wong, Albert J Fornace
INTRODUCTION: Due to dangers associated with potential accidents from nuclear energy and terrorist threats, there is a need for high-throughput biodosimetry to rapidly assess individual doses of radiation exposure. Lipidomics and metabolomics are becoming common tools for determining global signatures after disease or other physical insult and provide a "snapshot" of potential cellular damage. OBJECTIVES: The current study assesses changes in the nonhuman primate (NHP) serum lipidome and metabolome 7 days following exposure to ionizing radiation (IR)...
May 2016: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28219782/characterization-of-phospholipid-nitroxidation-by-lc-ms-in-biomimetic-models-and-in-h9c2-myoblast-using-a-lipidomic-approach
#3
Tânia Melo, Pedro Domingues, Teresa M Ribeiro-Rodrigues, Henrique Girão, Marcela A Segundo, M Rosário M Domingues
Under nitroxidative stress conditions, lipids are prone to be modified by reaction with reactive nitrogen species (RNS) and different modifications were reported to occur in fatty acids. However, in the case of phospholipids (PL) studied under nitroxidative stress conditions, only nitroalkene derivatives of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were reported when using both in vitro biomimetic conditions and in vivo model system of type 1 diabetes mellitus. Therefore, in order to further explore other nitroxidative modifications of PL, a biomimetic model of nitroxidation combined with liquid chromatography mass spectrometry (MS) and MS/MS approaches were used to characterize the nitrated and nitroxidized derivatives of PCs and PEs...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28219720/lipidomics-in-research-on-yeast-membrane-lipid-homeostasis
#4
Anton I P M de Kroon
Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae.
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219319/biomarkers-in-alzheimer-s-disease-recent-update
#5
Sushil Sharma, Walter Lipincott
Alzheimer disease (AD) is an age-related neurodegenerative disorder, characterized by loss of memory and cognitive function. It is the common cause of dementia in elderly and is a global health concern as the population of people aged 85 and older, is growing alarmingly. Although pharmacotherapy for the treatment of AD has improved, lot of work remains to treat this devastating disease. AD pathology begins even before the onset of clinical symptoms. Because therapies could be more effective if implemented early in the disease progression, it is highly prudent to discover reliable biomarkers, to detect its exact pathophysiology during pre-symptomatic stage...
February 20, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28216054/lipidomics-en-route-to-accurate-quantitation
#6
REVIEW
Sin Man Lam, He Tian, Guanghou Shui
Accurate quantitation is prerequisite for the sustainable development of lipidomics via enabling its applications in various biological and biomedical settings. In this review, the technical considerations and limitations of existent lipidomics technologies, particularly in terms of accurate quantitation; as well as the potential sources of errors along a typical lipidomics workflow that could ultimately give rise to quantitative inaccuracies will be addressed. Furthermore, the pressing need to exercise stricter definitions of terms and protocol standardization pertaining to quantitative lipidomics will be critically discussed, as quantitative accuracy may substantially impact upon the persevering development of lipidomics in the long run...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28212433/elevated-gm3-plasma-concentration-in-idiopathic-parkinson-s-disease-a-lipidomic-analysis
#7
Robin B Chan, Adler J Perotte, Bowen Zhou, Christopher Liong, Evan J Shorr, Karen S Marder, Un J Kang, Cheryl H Waters, Oren A Levy, Yimeng Xu, Hong Bin Shim, Itsik Pe'er, Gilbert Di Paolo, Roy N Alcalay
Parkinson's disease (PD) is a common neurodegenerative disease whose pathological hallmark is the accumulation of intracellular α-synuclein aggregates in Lewy bodies. Lipid metabolism dysregulation may play a significant role in PD pathogenesis; however, large plasma lipidomic studies in PD are lacking. In the current study, we analyzed the lipidomic profile of plasma obtained from 150 idiopathic PD patients and 100 controls, taken from the 'Spot' study at Columbia University Medical Center in New York. Our mass spectrometry based analytical panel consisted of 520 lipid species from 39 lipid subclasses including all major classes of glycerophospholipids, sphingolipids, glycerolipids and sterols...
2017: PloS One
https://www.readbyqxmd.com/read/28210754/targeted-lipidomics-profiling-of-acute-arsenic-exposure-in-mice-serum-by-on-line-solid-phase-extraction-stable-isotope-dilution-liquid-chromatography-tandem-mass-spectrometry
#8
Chao-Yu Chen, Pinpin Lin, Ming-Hsien Tsai, Hui-Ling Lee
Lipidomics is the area of study dedicated to the comprehensive analysis and characterization of the functions and metabolism of lipids in biological samples. One of the most comprehensively studied classes of lipids is polyunsaturated fatty acids (PUFAs). Eicosanoids are a series of bioactive lipid mediators that are metabolized from PUFAs and generated majorly from the precursor arachidonic acid. This study identified the profiles of target eicosanoids after acute exposure to arsenic. The principle objective was to determine and validate 10 eicosanoids in mouse serum using on-line solid-phase extraction integrated with liquid chromatography-electrospray tandem mass spectrometry...
February 16, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28209220/very-low-density-lipoprotein-associated-apolipoproteins-predict-cardiovascular-events-and-are-lowered-by-inhibition%C3%A2-of%C3%A2-apoc-iii
#9
Raimund Pechlaner, Sotirios Tsimikas, Xiaoke Yin, Peter Willeit, Ferheen Baig, Peter Santer, Friedrich Oberhollenzer, Georg Egger, Joseph L Witztum, Veronica J Alexander, Johann Willeit, Stefan Kiechl, Manuel Mayr
BACKGROUND: Routine apolipoprotein (apo) measurements for cardiovascular disease (CVD) are restricted to apoA-I and apoB. Here, the authors measured an unprecedented range of apolipoproteins in a prospective, population-based study and relate their plasma levels to risk of CVD. OBJECTIVES: This study sought to measure apolipoproteins directly by mass spectrometry and compare their associations with incident CVD and to obtain a system-level understanding of the correlations of apolipoproteins with the plasma lipidome and proteome...
February 21, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28208746/evidence-of-a-dha-signature-in-the-lipidome-and-metabolome-of-human-hepatocytes
#10
Veronica Ghini, Mattia Di Nunzio, Leonardo Tenori, Veronica Valli, Francesca Danesi, Francesco Capozzi, Claudio Luchinat, Alessandra Bordoni
Cell supplementation with bioactive molecules often causes a perturbation in the whole intracellular environment. Omics techniques can be applied for the assessment of this perturbation. In this study, the overall effect of docosahexaenoic acid (DHA) supplementation on cultured human hepatocyte lipidome and metabolome has been investigated using nuclear magnetic resonance (NMR) in combination with traditional techniques. The effect of two additional bioactives sharing with DHA the lipid-lowering effect-propionic acid (PRO) and protocatechuic acid (PCA)-has also been evaluated in the context of possible synergism...
February 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28207743/lipidomic-approach-for-stratification-of-acute-myeloid-leukemia-patients
#11
Adam Stefanko, Christian Thiede, Gerhard Ehninger, Kai Simons, Michal Grzybek
The pathogenesis and progression of many tumors, including hematologic malignancies is highly dependent on enhanced lipogenesis. De novo fatty-acid synthesis permits accelerated proliferation of tumor cells by providing membrane components but these may also alter physicochemical properties of lipid bilayers, which can impact signaling or even increase drug resistance in cancer cells. Cancer type-specific lipid profiles would permit us to monitor and interpret actual effects of lipid changes, potential fingerprints of individual tumors to be explored as diagnostic markers...
2017: PloS One
https://www.readbyqxmd.com/read/28202633/regional-changes-in-cns-and-retinal-glycerophospholipid-profiles-with-age-a-molecular-blueprint
#12
Blake R Hopiavuori, Martin-Paul Agbaga, Richard S Brush, Michael T Sullivan, William E Sonntag, Robert E Anderson
We present herein a quantitative molecular blueprint of the three major glycerophospholipid (GPL) classes phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE) in retina and six regions of the brain in C57Bl6 mice at 2, 10, and 26 months of age. We found an age-related increase in molecular species containing saturated and monoenoic fatty acids and an overall decrease in the longer chain polyunsaturated fatty acid (PUFA) molecular species across brain regions, with loss of docosahexaenoic acid (DHA)-containing molecular species as the most consistent and dramatic finding...
February 15, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28195557/biogenetic-and-morphofunctional-heterogeneity-of-mitochondria-the-case-of-synaptic-mitochondria
#13
Sergei V Fedorovich, Tatyana V Waseem, Ludmila V Puchkova
The mitochondria of different cells are different in their morphological and biochemical properties. These organelles generate free radicals during activity, leading inevitably to mitochondrial DNA damage. It is not clear how this problem is addressed in long-lived cells, such as neurons. We propose the hypothesis that mitochondria within the same cell also differ in lifespan and ability to divide. According to our suggestion, cells have a pool of 'stem' mitochondria with low metabolic activity and a pool of 'differentiated' mitochondria with significantly shorter lifespans and high metabolic activity...
February 14, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/28193460/multi-omics-a-critical-technical-perspective-on-integrative-lipidomics-approaches
#14
Dominik Kopczynski, Cristina Coman, Rene P Zahedi, Kristina Lorenz, Albert Sickmann, Robert Ahrends
During the past decades, high-throughput approaches for analyzing different molecular classes such as nucleic acids, proteins, metabolites, and lipids have grown rapidly. These approaches became powerful tools for getting a fundamental understanding of biological systems. Considering each approach and its results separately, relations and causal connections between these classes have no chance to be revealed, since only separate molecular snapshots are provided. Only a combined approach, not fully established yet, with the integration of the corresponding data, might yield a comprehensive and complete understanding of biological processes, such as crosstalk and interactions in signaling pathways...
February 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28193056/comprehensive-and-quantitative-analysis-of-polyphosphoinositide-species-by-shotgun-lipidomics-revealed-their-alterations-in-db-db-mouse-brain
#15
Chunyan Wang, Juan Pablo Palavicini, Miao Wang, Linyuan Chen, Kui Yang, Peter A Crawford, Xianlin Han
Polyphosphoinositides (PPI) play crucial roles in cellular signaling and functions. However, comprehensively determining the changed levels of these species during different cellular processes has faced difficulties. Herein, we applied a novel methylation pattern recognition and simulation approach, and we exploited newly derived fragmentation patterns of methylated PPI species for comprehensive analysis of PPI species including phosphate position(s) and fatty acyl chains capable of circumpassing previous limitations...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193003/on-mass-ambiguities-in-high-resolution-shotgun-lipidomics
#16
Chris Bielow, Guido Mastrobuoni, Marica Orioli, Stefan Kempa
Mass-spectrometry-based lipidomics aims to identify as many lipid species as possible from complex biological samples. Due to the large combinatorial search space, unambiguous identification of lipid species is far from trivial. Mass ambiguities are common in direct-injection shotgun experiments, where an orthogonal separation (e.g., liquid chromatography) is missing. Using the rich information within available lipid databases, we generated a comprehensive rule set describing mass ambiguities, while taking into consideration the resolving power (and its decay) of different mass analyzers...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192938/size-dependent-lipidomic-analysis-of-urinary-exosomes-from-patients-with-prostate-cancer-by-flow-field-flow-fractionation-and-nanoflow-liquid-chromatography-tandem-mass-spectrometry
#17
Joon Seon Yang, Jong Cheol Lee, Seul Kee Byeon, Koon Ho Rha, Myeong Hee Moon
Exosomes are membrane-bound extracellular vesicles involved in intercellular communication and tumor cell metastasis. In this study, flow field-flow fractionation (FlFFF) was utilized to separate urinary exosomes by size, demonstrating a significant difference in exosome sizes between healthy controls and patients with prostate cancer (PCa). Exosome fractions of different sizes were collected for microscopic analysis during an FlFFF run and evaluated with exosome marker proteins using Western blot analysis...
February 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28190990/the-translation-of-lipid-profiles-to-nutritional-biomarkers-in-the-study-of-infant-metabolism
#18
Animesh Acharjee, Philippa Prentice, Carlo Acerini, James Smith, Ieuan A Hughes, Ken Ong, Julian L Griffin, David Dunger, Albert Koulman
INTRODUCTION: Links between early life exposures and later health outcomes may, in part, be due to nutritional programming in infancy. This hypothesis is supported by observed long-term benefits associated with breastfeeding, such as better cognitive development in childhood, and lower risks of obesity and high blood pressure in later life. However, the possible underlying mechanisms are expected to be complex and may be difficult to disentangle due to the lack of understanding of the metabolic processes that differentiate breastfed infants compared to those receiving just formula feed...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28188148/analysis-of-sphingolipids-in-human-corneal-fibroblasts-from-normal-and-keratoconus-patients
#19
Hui Qi, Shrestha Priyadarsini, Sarah E Nicholas, Akhee Sarker-Nag, Jeremy Allegood, Charles E Chalfant, Nawajes A Mandal, Dimitrios Karamichos
The pathophysiology of human keratoconus (KC), a bilateral progressive corneal disease, leading to protrusion of the cornea, stromal thinning, and scarring is not well understood. In this study we investigated a novel Sphingolipid (SPL) signaling pathway through which KC may be regulated. Using human corneal fibroblasts (HCF) and human keratoconus cells (HKC) we examined the SPL pathway modulation. Both cell types were stimulated by the three TGF-β isoforms: TGF-B1 (T1), B2 (T2), and B3 (T3). All samples were analyzed using lipidomics, and real time-PCR...
February 10, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28187425/computational-lipidomics-and-lipid-bioinformatics-filling-in-the-blanks
#20
Josch Pauling, Edda Klipp
Lipids are highly diverse metabolites of pronounced importance in health and disease. While metabolomics is a broad field under the omics umbrella that may also relate to lipids, lipidomics is an emerging field which specializes in the identification, quantification and functional interpretation of complex lipidomes. Today, it is possible to identify and distinguish lipids in a high-resolution, high-throughput manner and simultaneously with a lot of structural detail. However, doing so may produce thousands of mass spectra in a single experiment which has created a high demand for specialized computational support to analyze these spectral libraries...
December 22, 2016: Journal of Integrative Bioinformatics
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