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https://www.readbyqxmd.com/read/29773834/embryonic-pou5f1-is-required-for-expanded-bovine-blastocyst-formation
#1
Bradford W Daigneault, Sandeep Rajput, George W Smith, Pablo J Ross
POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29768247/oocyte-triggering-day-progesterone-levels-and-endometrial-appearance-in-normoresponders-undergoing-ivf-icsi-cycles-a-hypothesis-and-a-study-protocol
#2
Charalampos Siristatidis, Panagiotis Drakopoulos, Paraskevi Vogiatzi, Vasilios Karageorgiou, George Daskalakis
In this report, we propose a study protocol capable of improving IVF outcomes in subfertile women with expected normal ovarian response. This proposal derives from conflicting published data and observations in our daily practice, concerning the negative impact of progesterone (P4) elevation at the day of oocyte triggering on pregnancy outcomes. Our hypothesis points to the combination of two previous "suspects" of reduced success after assisted reproduction techniques (ART) - the endometrium ultrasonographic parameters and P4 elevation at the day of oocyte triggering on their impact on pregnancy outcomes...
May 16, 2018: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/29761478/pain-relief-for-women-undergoing-oocyte-retrieval-for-assisted-reproduction
#3
REVIEW
Irene Kwan, Rui Wang, Emily Pearce, Siladitya Bhattacharya
BACKGROUND: Various methods of conscious sedation and analgesia (CSA) have been used during oocyte retrieval for assisted reproduction. The choice of agent has been influenced by the quality of sedation and analgesia and by concerns about possible detrimental effects on reproductive outcomes. OBJECTIVES: To assess the effectiveness and safety of different methods of conscious sedation and analgesia for pain relief and pregnancy outcomes in women undergoing transvaginal oocyte retrieval...
May 15, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29757581/transmyometrial-embryo-transfer-as-a-useful-method-to-overcome-difficult-embryo-transfers-a-single-center-retrospective-study
#4
Janisse Ferreri Dos Anjos, Emma Gabriela Portillo Osorio, Joana Peñarrubia, Ester Vidal, Francisco Fábregues Gasol
OBJECTIVE: Pregnancy after an embryo transfer depends largely on embryo quality, endometrial receptivity, and the technique used in the embryo transfer. Embryo transfers have been reported as inevitably traumatic and difficult for 5-7% of patients in assisted reproduction treatment. In these cases, transmyometrial embryo transfer should be considered as a suitable method to overcome difficult embryo transfers. The aim of this study was to report our experience with this technique and analyze its causes, results and complications...
May 14, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29749276/cryopreservation-of-low-number-of-human-spermatozoa-which-is-better-vapor-phase-or-direct-submerging-in-liquid-nitrogen
#5
Akram Hosseini, Mohammad Ali Khalili, Ali Reza Talebi, Azam Agha-Rahimi, Saeed Ghasemi-Esmailabad, Bryan Woodward, Nahid Yari
Cryopreservation and subsequent survival of semen samples with low numbers of human spermatozoa in assisted reproductive technology (ART) facilities can be challenging. The aim of this study was to compare the quality of warmed human spermatozoa following vitrification using direct submerging (DS) in liquid nitrogen (LN2 ) or with LN2 vapour (V). Normozoospermic ejaculates were prepared by the swim-up technique and the motile sperm fraction was divided into three groups: (i) fresh (control); (ii) DS methods in LN2 (DS Group); or (iii) cryopreserved in V (Group V)...
May 11, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29741637/microscopic-analysis-of-sperm-movement-links-to-mechanisms-and-protein-components
#6
Kazuo Inaba, Kogiku Shiba
Cilia and flagella are cell machines that power hydrodynamic forces by fast beating. They are composed of bundles of 9 + 2 microtubules that associate with several protein structures including axonemal dyneins and their regulators. Spermatozoa are single cells with in most cases a single flagellum and are good cell models to study how flagellar movements are regulated and linked to cell behavior. In addition, the assessment of sperm motility is an important diagnostic tool for evaluating male fertility in human reproductive medicine, and in livestock and fisheries sciences...
May 8, 2018: Microscopy
https://www.readbyqxmd.com/read/29741247/prenatal-and-preimplantation-diagnosis-of-hemoglobinopathies
#7
REVIEW
C Vrettou, G Kakourou, T Mamas, J Traeger-Synodinos
The hemoglobinopathies, as a group, are one of the most common serious monogenic diseases in the world. An accepted and widely adopted approach to reduce the number of new cases involves carrier-screening programs, with the option of prenatal diagnosis (PND) or preimplantation diagnosis (preimplantation genetic testing for monogenic disease, PGT-M) for carrier couples. The aim of PND is to provide an accurate result as early in pregnancy as possible, which necessitates prior identification of the parental disease-causing mutations, as well as safe and timely biopsy of fetal material...
May 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29740747/mothers-in-same-sex-relationships-describe-the-process-of-forming-a-family-as-a-stressful-journey-in-a-heteronormative-world-a-swedish-grounded-theory-study
#8
Heléne Appelgren Engström, Elisabet Häggström-Nordin, Catrin Borneskog, Anna-Lena Almqvist
Objectives The aim of this study was to gain insight into how women in same-sex relationships experience the process of forming a family through the use of assisted reproduction technique (ART), from planning the pregnancy to parenthood, and their experience of parental support from healthcare professionals. Methods The participants were 20 women in a same-sex relationship who had conceived through ART at a Swedish clinic. Semi-structured interviews including open questions about pregnancy, parenthood and support from healthcare professionals were conducted...
May 8, 2018: Maternal and Child Health Journal
https://www.readbyqxmd.com/read/29734426/changes-in-the-membrane-carbohydrates-from-sperm-cryopreserved-with-dimethylsulfoxide-or-polyvinylpyrrolidone-of-red-tailed-hawk-buteo-jamaicencis
#9
J A Herrera, G Calderon, C Cruz, M A Avila, G E Quintero, R C Fierro
BACKGROUND: That cryopreservation can induce alterations in sperm. OBJECTIVE: The goal of this study was to evaluate sperm quality and distribution of N-acetylglucosamine, sialic acid and mannose residues in sperm cryopreserved of red-tailed hawk (Buteo jamaicensis). MATERIALS AND METHODS: We studied twenty samples of ejaculated semen for each cryoprotectant dimethylsulfoxide or polyvinylpyrrolidone. Carbohydrate identification was carried out with lectins Triticum vulgaris agglutinin to N-acetylglucosamine and sialic acid and Concanavalia ensiformis for mannose residues...
July 2017: Cryo Letters
https://www.readbyqxmd.com/read/29728797/potential-of-testicular-sperm-to-support-embryonic-development-to-the-blastocyst-stage-is-comparable-to-that-of-ejaculated-sperm
#10
Sun-Hee Lee, Chan Woo Park, Yong-Pil Cheon, Chun Kyu Lim
PURPOSE: Recent studies have shown that improved clinical outcomes can be achieved by transferring blastocysts rather than cleavage-stage embryos. However, blastocyst transfer is not performed in all patients. The aim of this study was to compare clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles using testicular sperm (TE) with those of ICSI cycles using ejaculated sperm (EJ). METHODS: ICSI was performed using EJ in 141 cycles and TE in 37 cycles...
May 5, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29723568/family-history-is-under-estimated-in-children-with-isolated-hypospadias-a-french-multicenter-report-of-88-families
#11
Margot Ollivier, Francoise Paris, Pascal Philibert, Sarah Garnier, Amandine Coffy, Nadège Fauconnet-Servant, Mirna Haddad, Jean Michel Guys, Rachel Reynaud, Alice Faure, Thierry Merrot, Kathy Wagner, Jean Bréaud, Jean Stéphane Valla, Eric Dobremez, Laura Gaspari, Jean-Pierre Daures, Charles Sultan, Nicolas Kalfa
Whereas familial forms of complex disorders/differences of sex development have been widely reported, data regarding isolated hypospadias are sparse and a family history is thought to be less frequent. We aimed 1-to determine the frequency of hypospadias in families of hypospadiac boys 2-to determine if theses familial forms exhibit a particular phenotype 3-to evaluate the prevalence of genetic defects of the main candidate genes. A prospective inclusion of 395 hypospadiac boys screened for family history with a standardized questionnaire, extensive clinical description, family tree and sequencing of AR, SF1, SRD5A2 and MAMLD1 was performed...
April 30, 2018: Journal of Urology
https://www.readbyqxmd.com/read/29722907/use-of-somatic-cell-banks-in-the-conservation-of-wild-felids
#12
REVIEW
Érika A Praxedes, Alana A Borges, Maria V O Santos, Alexsandra F Pereira
The conservation of biological resources is an interesting strategy for the maintenance of biodiversity, especially for wild felids who are constantly threatened with extinction. For this purpose, cryopreservation techniques have been used for the long-term storage of gametes, embryos, gonadal tissues, and somatic cells and tissues. The establishment of these banks has been suggested as a practical approach to the preservation of species and, when done in tandem with assisted reproductive techniques, could provide the means for reproducing endangered species...
May 3, 2018: Zoo Biology
https://www.readbyqxmd.com/read/29714057/large-for-gestational-age-and-macrosomia-in-singletons-born-after-frozen-thawed-embryo-transfer-fet-in-assisted-reproductive-technology-art
#13
REVIEW
Sine Berntsen, Anja Pinborg
Increase in success rates with frozen embryo transfer (FET) and reduced risk of ovarian hyperstimulation syndrome (OHSS) and multiple pregnancies has lead to a steady rise in FET. Further, FET is associated with lower risk of prematurity and low birth weight in singletons, when compared with fresh transfer. However, there is a growing concern of increased risk of large for gestational (LGA) and/or macrosomic children after FET. Macrosomic/LGA births have a higher risk of cesarean section, fetal hypoxia and stillbirth...
May 1, 2018: Birth Defects Research
https://www.readbyqxmd.com/read/29713537/a-systematic-review-on-sperm-dna-fragmentation-in-male-factor-infertility-laboratory-assessment
#14
Manesh Kumar Panner Selvam, Ashok Agarwal
Objective: To review sperm DNA fragmentation (SDF) testing as an important sperm function test in addition to conventional semen analysis. High SDF is negatively associated with semen quality, the fertilisation process, embryo quality, and pregnancy outcome. Over recent decades, different SDF assays have been developed and reviewed extensively to assess their applicability and accuracy as advanced sperm function tests. Amongst them, the standardisation of the terminal deoxynucleotidyl transferased UTP nick-end labelling (TUNEL) assay with a bench top flow cytometer in clinical practice deserves special mention with a threshold value of 16...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29713533/role-of-sperm-dna-fragmentation-in-male-factor-infertility-a-systematic-review
#15
Chak-Lam Cho, Ashok Agarwal
Objective: To summarise the latest evidence on the role of sperm DNA fragmentation (SDF) in male factor infertility, as SDF has been emerging as a valuable tool for male infertility evaluation. Methods: A search of PubMed was conducted using the keywords 'sperm DNA fragmentation' and 'male infertility'. Studies in languages other than English were excluded. All identified studies were screened and clinical studies in humans were included. Results: In all, 150 articles were included for analysis...
March 2018: Arab Journal of Urology
https://www.readbyqxmd.com/read/29708090/couples-psychological-adjustment-to-twin-parenthood-mode-of-conception-spontaneous-versus-assisted-reproduction-and-gender-differences
#16
Iva Tendais, Bárbara Figueiredo, Catarina Canário, David A Kenny
AimTo examine whether mode of conception and gender are associated with parents' psychological adjustment across the transition to twin parenthood. BACKGROUND: There is limited knowledge on the psychological adjustment of couples to twin parenthood during pregnancy and early postpartum, especially for fathers. The available research suggests that first-time mothers of twins conceived by assisted reproduction techniques (ART) may experience lower psychosocial well-being than mothers of spontaneously conceived (SC) twins...
April 30, 2018: Primary Health Care Research & Development
https://www.readbyqxmd.com/read/29698073/surgical-extraction-of-viable-hen-gallus-gallus-domesticus-follicles-for-in-vitro-fertilization
#17
Juan J Perez-Rivero, Angel R Lozada-Gallegos, Jose A Herrera-Barragán
Assisted reproduction techniques in birds have been developed for zootechnical purposes and have been adapted for use in conservation of wild bird species. To develop a technique for obtaining follicles in live hens, 5 Rhode Island red hens ( Gallus gallus domesticus) were anesthetized, and abdominal ultrasound was performed to confirm the presence of ovarian follicles. A left celiotomy then was performed to obtain follicles in different stages of maturation for in vitro fertilization. The follicles were located by digital exploration, then extracted by isolating each follicle with the index finger of each hand, holding it by the stigma, and then applying slight traction towards the exterior of the coelomic cavity until the follicle separated from the ovary...
March 2018: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/29697138/follicular-flushing-during-oocyte-retrieval-in-assisted-reproductive-techniques
#18
REVIEW
Ektoras X Georgiou, Pedro Melo, Julie Brown, Ingrid E Granne
BACKGROUND: Follicular aspiration under transvaginal ultrasound guidance is routinely performed as part of assisted reproductive technology (ART) to retrieve oocytes for in vitro fertilisation (IVF). However, controversy as to whether follicular flushing following aspiration yields a larger number of oocytes and hence is associated with greater potential for pregnancy than aspiration only is ongoing. OBJECTIVES: To assess the safety and efficacy of follicular flushing as compared with aspiration only performed in women undergoing ART...
April 26, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29696942/challenges-of-fertility-preservation-in-leukemia-patients
#19
Moran Shapira, Hila Raanani, Sanaz Derech Chaim, Dror Meirow
An ongoing increase in survival rates among young leukemia patients is accompanied by a growing attention to possible long-term complications such as chemotherapy induced ovarian insufficiency and infertility. Therefore, an important element of the management of these patients is fertility preservation, which is challenged by considerations specific for leukemia. Such considerations include prepuberty, poor medical condition on presentation and the need for urgent chemotherapy, all of which may preclude the use of conventional assisted reproductive technologies...
April 24, 2018: Minerva Ginecologica
https://www.readbyqxmd.com/read/29690805/correction-to-karami-et-al-the-association-of-placenta-previa-and-assisted-reproductive-techniques-a-meta-analysis
#20
(no author information available yet)
No abstract text is available yet for this article.
April 24, 2018: Journal of Maternal-fetal & Neonatal Medicine
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