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https://www.readbyqxmd.com/read/29339137/oxygen-induced-alterations-in-the-expression-of-chromatin-modifying-enzymes-and-the-transcriptional-regulation-of-imprinted-genes
#1
William M Skiles, Avery Kester, Jane H Pryor, Mark E Westhusin, Michael C Golding, Charles R Long
Embryo culture and assisted reproductive technologies have been associated with a disproportionately high number of epigenetic abnormalities in the resulting offspring. However, the mechanisms by which these techniques influence the epigenome remain poorly defined. In this study, we evaluated the capacity of oxygen concentration to influence the transcriptional control of a selection of key enzymes regulating chromatin structure. In mouse embryonic stem cells, oxygen concentrations modulated the transcriptional regulation of the TET family of enzymes, as well as the de novo methyltransferase Dnmt3a...
January 12, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29334209/comparison-of-the-effects-of-vitrification-on-gene-expression-of-mature-mouse-oocytes-using-cryotop-and-open-pulled-straw
#2
Fardin Amidi, Zahra Khodabandeh, Mohamad Hossain Nori Mogahi
Background: Oocyte cryopreservation is an essential part of the assisted reproductive technology (ART), which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems-Cryotop and Open Pulled Straw (OPS)-on mature oocytes gene expressions. MATERIALS AND METHODS: In this experimental study, the survival rate of metaphase II (MII) mouse oocytes were assessed after cryopreservation by vitrification via i. OPS or ii...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29332425/dermatological-manifestations-in-patients-undergoing-in-vitro-fertilisation-a-prospective-study
#3
Aradhana Sood, Suvash Sahu, Sandeep Karunakaran, Rajneesh K Joshi, Deep Kumar Raman
BACKGROUND: Changing sociodemographic patterns with an increase in the age of childbirth have affected fertility rates worldwide. With advancing reproductive medicine, assisted reproductive techniques (ARTs) are becoming common. While dermatological manifestations in normal pregnancies have been well documented, there is a paucity of data regarding cutaneous manifestations in patients undergoing ART. OBJECTIVES: The objectives of our study were to estimate the incidence and types of dermatological manifestations in patients undergoing in vitro fertilisation (IVF) and to study their associations with age, type of infertility, and outcome of the procedure...
January 1, 2018: Journal of Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/29317175/premature-progesterone-rise-in-art-cycles
#4
REVIEW
B Lawrenz, L Melado, H M Fatemi
Premature rise of progesterone during the late follicular phase in stimulated IVF cycles is a frequent event and its effect on the endometrial receptivity and on the ART (Assisted Reproductive Technique) - outcome has become a matter of intense debate and research. An emerging body of evidence demonstrates that premature progesterone rise does have a negative impact on the outcome of the ART-success. Until now, the exact cause of progesterone elevation is not fully clear, however lately published studies points to the fact, that premature progesterone elevation might be caused by enhanced FSH stimulation...
January 6, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29306491/effect-of-air-bubbles-localization-and%C3%A2-migration-after-embryo-transfer%C3%A2-on%C3%A2-assisted-reproductive-technology-outcome
#5
Cem Fıçıcıoğlu, Pınar Özcan, Melis G Koçer, Mert Yeşiladalı, Oya Alagöz, Gülçin Özkara, Ahter T Tayyar, Çiğdem Altunok
OBJECTIVE: To evaluate the effect of embryo flash position and movement of the air bubbles at 1 and 60 minutes after ET on clinical pregnancy rates (PRs). DESIGN: Prospective clinical trial. SETTING: University fertility clinic. PATIENT(S): A total of 230 fresh ultrasound-guided ETs performed by a single physician (C.F.) at the IVF center of Yeditepe University Hospital between September 2016 and February 2017 were included...
January 3, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29304839/new-national-outcome-data-on-fresh-versus-cryopreserved-donor-oocytes
#6
Vitaly A Kushnir, Sarah K Darmon, David H Barad, Norbert Gleicher
BACKGROUND: Improvements in oocyte cryopreservation techniques and establishment of cryopreserved donor oocyte banks have led to improved access to and lower cost of donor oocytes, upending the traditional practice of fresh oocyte donation. The objective of this study was to examine national trends in utilization and live birth rates with fresh versus cryopreserved donor oocytes. METHODS: A retrospective analysis of 2013 through 2015 aggregate U.S. national data reported by the Society for Assisted Reproductive Technology which included 30,160 IVF cycles with either fresh or cryopreserved donor oocytes was performed...
January 5, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29297236/cleavage-stage-embryo-micromanipulation-in-the-clinical-setting
#7
Iman Halvaei, Shahin Ghazali, Stefania A Nottola, Mohammad Ali Khalili
Embryo micromanipulation was developed after introduction of microinjection to overcome infertility. Embryo micromanipulation may be performed at any embryo stage from pronuclear to blastocyst. The technique started out as basic and turned out to be increasingly more complex. Embryo micromanipulation at the cleavage-stage includes a wide range of techniques, from opening the zona pellucida in order to improve the chance of implantation, to removing detrimental components from the embryo to enhance embryo development or blastomeres for preimplantation genetic diagnosis and embryo splitting...
January 3, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29287896/mitochondria-targeted-antioxidant-mitotempo-improves-the-post-thaw-sperm-quality
#8
Xilan Lu, Yingchun Zhang, Hongwei Bai, Jingjing Liu, Juntao Li, Bin Wu
Human spermatozoa cryopreservation is an important means of assisted reproductive technology and male fertility preservation. Although this technique is particularly useful, sperm cryopreservation significantly reduces the quality of spermatozoa after freezing and thawing. The objective of the study is to examine the efficacy of mitochondria-targeted antioxidant MitoTEMPO in improving sperm quality during semen cryopreservation processes. Semen samples were collected and cryopreserved in extenders containing different concentrations (0...
December 26, 2017: Cryobiology
https://www.readbyqxmd.com/read/29277366/role-of-withania-somnifera-ashwagandha-in-the-management-of-male-infertility
#9
REVIEW
Pallav Sengupta, Ashok Agarwal, Maria Pogrebetskaya, Shubhadeep Roychoudhury, Damayanthi Durairajanayagam, Ralf Henkel
To manage male infertility caused by hormonal imbalance, infections and other predicaments, multifarious treatment strategies are emerging worldwide. Contemporary treatments, such as assisted reproductive techniques, are costly with low success rates of only 10-30%; however, herbal remedies are gaining more attention as an alternative or supplementary therapeutic modality for male infertility. The beneficial effects induced by oral intake of the roots of a small evergreen shrub, Withania sominifera (Ashwagandha) on semen quality of infertile men have previously been studied...
December 7, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29249329/comparative-ovarian-function-and-reproductive-monitoring-of-endangered-mammals
#10
Janine L Brown
The ability to track gonadal function is facilitated by the use of endocrine and ultrasound techniques, both of which are important tools for optimizing reproduction and ensuring sustainability of fragile populations. With so many species now endangered, captive breeding is increasingly viewed as a means to sustain important insurance populations. As reproduction is key to species survival, understanding how to control and monitor ovarian function is vital. Through decades of study, we now have a greater understanding of the diversity, and plasticity, of reproductive mechanisms across taxa...
December 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29241919/does-the-freeze-all-policy-allow-for-a-better-outcome-in-assisted-reproductive-techniques-than-the-use-of-fresh-embryo-transfers-a%C3%A2-retrospective-study-on-cumulative-live-birth-rates
#11
Jui-Chun Chang, Ming-Jer Chen, Hwa-Fen Guu, Ya-Fang Chen, Yu-Chiao Yi, Hsiao-Fan Kung, Li-Yu Chen, Min-Min Chou
OBJECTIVE: There are increasing concerns regarding the adverse effects associated with control ovarian hyperstimulation (COH) in both endometrial and uterine environments. With the "segmentation treatment policy" of assisted reproductive techniques (ART), endometrial problems may be obviated through embryo cryopreservation. However, it remains unclear if the "freeze-all policy" offers a better outcome when compared with fresh embryo transfer (ET). To clarify this, we compared the cumulative live birth rates (CLBRs) between these two patient populations...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29233501/does-ovarian-stimulation-regimen-affect-ivf-outcome-a-two-centre-real-world-retrospective-study-using-predominantly-cleavage-stage-single-embryo-transfer
#12
Per-Olof Karlström, Jan Holte, Nermin Hadziosmanovic, Kenny A Rodriguez-Wallberg, Jan I Olofsson
In this study, ovarian stimulation using highly purified human menopausal gonadotrophin (HP-HMG) and recombinant FSH (rFSH) for IVF were compared in two large assisted reproduction technique centres in Sweden. A total of 5902 women underwent 9631 oocyte retrievals leading to 8818 embryo transfers (7720 on day 2): single embryo transfers (74.2%); birth rate per embryo transfer (27.7%); multiple birth rate (5.0%); incidence of severe ovarian hyperstimulation syndrome (0.71%). Compared with ovarian stimulation with rFSH, women who received HP-HMG were older, had higher dosages of gonadotrophins administered, fewer oocytes retrieved and more embryos transferred...
October 20, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29216686/congenital-bilateral-absence-of-the-vas-deferens-as-an-atypical-form-of-cystic-fibrosis-reproductive-implications-and-genetic-counseling
#13
REVIEW
D A S de Souza, F R Faucz, L Pereira-Ferrari, V S Sotomaior, S Raskin
Congenital bilateral absence of the vas deferens (CBAVD) is found in 1% to 2% of males with infertility and is present in 6% of obstructive azoospermia cases. Nearly 95% of men with cystic fibrosis (CF, an autosomal recessive disorder) have CBAVD. There are genetic links between CBAVD and CF. Some mutations in the gene encoding cystic fibrosis transmembrane conductance regulator (CFTR) can lead to CBAVD as a monosymptomatic form of CF. With the use of assisted reproductive techniques (ART), especially testicular or epididymal sperm aspiration, intracytoplasmic sperm injection, and in vitro fertilization, it is possible that men with CBAVD can produce offspring...
December 7, 2017: Andrology
https://www.readbyqxmd.com/read/29216009/the-first-trimester-aneuploidy-biochemical-markers-in-ivf-icsi-patients-have-no-additional-benefit-compared-to-spontaneous-conceptions-in-the-prediction-of-pregnancy-complications
#14
Iwona Szymusik, Przemyslaw Kosinski, Katarzyna Kosinska-Kaczynska, Damian Warzecha, Anetta Karwacka, Bartosz Kaczynski, Miroslaw Wielgos
OBJECTIVES: The aim of this study was to determine if the levels of biochemical aneuploidy markers in in vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI) pregnancies differ from those in spontaneous pregnancies and to verify if biochemical markers could predict pregnancy outcome in IVF/ICSI gestations. METHODS: This was a prospective observational study performed in a group of 551 patients who underwent a combined first trimester prenatal screening (ultrasound scan and serum markers)...
December 7, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/29207452/congenital-malformations-in-infants-of-mothers-undergoing-assisted-reproductive-technologies-a-systematic-review-and-meta-analysis-study
#15
Hayedeh Hoorsan, Parvin Mirmiran, Shahla Chaichian, Yousef Moradi, Roza Hoorsan, Fatemeh Jesmi
Objectives: This meta-analysis aimed to evaluate congenital malformations in infants conceived by assisted reproductive techniques (ART), compared with infants conceived spontaneously. Methods: In this study, available resources searched to find relevant articles included PubMed, ScienceDirect, Scopus, Google Scholar, Cochrane, ProQuest, Iranmedex, Magiran, and Scientific Information Database. After extracting the necessary information from evaluated articles, meta-analysis on the articles' data was performed using Stata version 11...
November 2017: Journal of Preventive Medicine and Public Health, Yebang Ŭihakhoe Chi
https://www.readbyqxmd.com/read/29203384/do-endometriomas-grow-during-ovarian-stimulation-for-assisted-reproduction-a-three-dimensional-volume-analysis-before-and-after-ovarian-stimulation
#16
Ayse Seyhan, Bulent Urman, Engin Turkgeldi, Baris Ata
Whether endometriomas grow because of supraphysiological oestradiol levels attained during ovarian stimulation for assisted reproduction techniques is a concern. In this prospective study, 25 women with 28 endometriomas underwent three-dimensional ultrasound using sono-automated volume calculation software. Endometrioma volume was measured on the first day of gonadotrophin injection (V1) and the day of ovulation trigger (V2). Nine (36%) women were stimulated in a gonadotrophin releasing hormone antagonist protocol (GnRH), 13 (52%) in a long, and three (12%) in an ultra-long GnRH agonist protocol...
November 17, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29199153/retrospective-assessment-of-an-assisted-reproductive-technology-method
#17
Siham Aboulmakarim, Amal Benbella, Houyam Hardizi, Rachid Bezad
The validation of methods in medical biology is a fundamental step in which the laboratory defines its analytical objectives, characterizes the performance of the technique according to the level of quality it fixes and discusses the results obtained. The objective of this work is to contribute to respect the requirements of ISO 15189 in terms of performance verification of a manual qualitative technique of medically assisted procreation (PMA): morphological identification of the oocyte, the zygote and the embryo, harmonization of professional practices and assessment of competences by associating the bibliographic approach and above all by justifying its choices...
December 4, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29186987/perurethral-transvesical-route-for-oocytes-retrieval-an-old-technique-for-a-new-indication-in-oncofertility
#18
Mohamed Khrouf, Marouen Braham, Maha Bouyahia, Khadija Kacem-Berejeb, Hanène Elloumi-Chaabane, Amel Zhioua, Fethi Zhioua
We aimed to evaluate the safety and efficiency of the peruretheral transvesical oocyte retrieval in oncofertility. We conducted a retrospective comparative study in our assisted reproductive technologies center. STUDY GROUP: 28 pubertal young women affected by malignancies, referred for fertility preservation and refusing transvaginal (TV) procedure. CONTROL GROUP: 28 infertile patients, aged less than 25 years, who have undergone in vitro fertilization with TV oocyte retrieval...
December 2017: Future Oncology
https://www.readbyqxmd.com/read/29164811/congenital-heart-defects-in-ivf-icsi-pregnancy-a-systematic-review-and-meta-analysis
#19
REVIEW
Veronica Giorgione, Fabio Parazzini, Vlasta Fesslova, Sonia Cipriani, Massimo Candiani, Annalisa Inversetti, Cristina Sigismondi, Francesca Tiberio, Paolo Cavoretto
OBJECTIVE: There is no consensus in current practice guidelines whether IVF/ICSI conception represents an indication for performing a fetal echocardiogram. The aim of the study is to assess whether CHD occurs more often with the use of in-vitro-fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) techniques as compared to spontaneous pregnancies. METHODS: A systematic search for studies in PubMed/MEDLINE, Embase and Scopus from inception to September 2017 was conducted...
November 22, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29164653/gradient-sperm-selection-for-reproductive-techniques-in-cattle-is-isolate-a-suitable-replacement-for-percoll
#20
B Sepúlveda, M E Arias, L Aguila, F Zambrano, R Sánchez, R Felmer
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate(®) was a suitable substitute for Percoll(®) for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (ΔΨm) were assessed by flow cytometry...
November 21, 2017: Andrologia
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