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https://www.readbyqxmd.com/read/28734977/modulation-of-the-interaction-between-human-p450-3a4-and-b-megaterium-reductase-via-engineered-loops
#1
Silvia Castrignanò, Serena D'Avino, Giovanna Di Nardo, Gianluca Catucci, Sheila J Sadeghi, Gianfranco Gilardi
Chimerogenesis involving cytochromes P450 is a successful approach to generate catalytically self-sufficient enzymes. However, the connection between the different functional modules should allow a certain degree of flexibility in order to obtain functional and catalytically efficient proteins. We previously applied the molecular Lego approach to develop a chimeric P450 3A4 enzyme linked to the reductase domain of P450 BM3 (BMR). Three constructs were designed with the connecting loop containing no glycine, 3 glycine or 5 glycine residues and showed a different catalytic activity and coupling efficiency...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734901/gastroresistant-oral-peptide-for-fluorescence-imaging-of-colonic-inflammation
#2
Paola Luciani, Ander Estella-Hermoso de Mendoza, Tommaso Casalini, Silvia Lang, Kirstin Atrott, Marianne R Spalinger, Anna Pratsinis, Jens Sobek, Isabelle Frey-Wagner, Jens Schumacher, Jean-Christophe Leroux, Gerhard Rogler
The use of molecular markers for inflammation in the gastrointestinal tract could empower optical imaging modalities for early diagnosis and eventually personalized timely treatments. A major hurdle to the widespread use of functional fluorescence imaging is the absence of suitable contrast agents, in particular to be administered via the oral route due to the usual proteolytic susceptibility of the biomarkers. By designing a retro-inverso peptide, starting from a previously described sequence specific for N-cadherin, we achieved resistance to gastrointestinal degradation and even slightly improved specificity towards the target, both in ex vivo and in vivo experimental colitis...
July 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28734753/cranial-bone-structure-in-children-with-sagittal-craniosynostosis-relationship-with-surgical-outcomes
#3
Naiara Rodriguez-Florez, Amel Ibrahim, J Ciaran Hutchinson, Alessandro Borghi, Greg James, Owen J Arthurs, Patrizia Ferretti, David Dunaway, Silvia Schievano, N U Owase Jeelani
BACKGROUND: While spring-assisted cranioplasty has become a widespread technique to correct scaphocephaly in children with sagittal synostosis, predicting head shape changes induced by the gradual opening of the springs remains challenging. This study aimed to explore the role of cranial bone structure on surgical outcomes. METHODS: Patients with isolated sagittal synostosis undergoing spring-assisted cranioplasty at GOSH (London, UK) were recruited (n = 18, age: 3-8 months)...
June 27, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28734711/-bifidobacterium-scardovii-isolated-from-2-consecutive-urine-samples
#4
Daiana Guevara Nuñez, Adriana N De Paulis, Eugenia Bertona, Miguel Ángel Gutiérrez, Carlos A Vay, Juan P Suárez, Silvia C Predari
Bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by CO2 and anaerobiosis. Exceptionally it has been associated with infections in humans. An elderly male patient with a urinary tract infection due to B. scardovii and Enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. The bacillus did not grow on standard media, but on chocolate agar incubated in CO2 and on supplemented Brucella agar in an anaerobic atmosphere, incubated for 72h at 35°C...
July 19, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28734679/assessment-of-biodistribution-using-mesenchymal-stromal-cells-algorithm-for-study-design-and-challenges-in-detection-methodologies
#5
Blanca Reyes, Maria Isabel Coca, Margarita Codinach, María Dolores López-Lucas, Anna Del Mazo-Barbara, Marta Caminal, Irene Oliver-Vila, Valentín Cabañas, Silvia Lope-Piedrafita, Joan García-López, José M Moraleda, Cesar G Fontecha, Joaquim Vives
BACKGROUND AIMS: Biodistribution of candidate cell-based therapeutics is a critical safety concern that must be addressed in the preclinical development program. We aimed to design a decision tree based on a series of studies included in actual dossiers approved by competent regulatory authorities, noting that the design, execution and interpretation of pharmacokinetics studies using this type of therapy is not straightforward and presents a challenge for both developers and regulators...
July 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28734004/comparison-of-preferences-of-healthcare-professionals-and-ms-patients-for-attributes-of-disease-modifying-drugs-a-best-worst-scaling
#6
Ingrid E H Kremer, Silvia M A A Evers, Peter J Jongen, Mickaël Hiligsmann
BACKGROUND: The choice between disease-modifying drugs (DMDs) for the treatment of multiple sclerosis (MS) becomes more often a shared decision between the patient and the neurologist and MS nurse. This study aimed to assess which DMD attributes are most important for the healthcare professionals in selecting a DMD for a patient. Subsequently, within this perspective, the neurologists' and nurses' perspectives were compared. Lastly, the healthcare professionals' perspective was compared with the patients' perspective to detect any differences that may need attention in the communication about DMDs...
July 21, 2017: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/28733783/perianth-organs-in-nymphaeaceae-comparative-study-on-epidermal-and-structural-characters
#7
Lucía Melisa Zini, Beatriz Gloria Galati, María Silvia Ferrucci
The perianth organs of six species of Nymphaeaceae, representing Euryale, Nymphaea and Victoria, were studied on the basis of macroscopical, micromorphological, and anatomical characters. The aims were to determine whether perianth is differentiated among tepal whorls considering the presence of sepaloid and petaloid characters, and to evaluate the occurrence of both features in individual tepals. Selected perianth series were examined macroscopically, with light microscopy, and scanning electron microscopy...
July 21, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28733610/environmental-impact-of-omnivorous-ovo-lacto-vegetarian-and-vegan-diet
#8
Alice Rosi, Pedro Mena, Nicoletta Pellegrini, Silvia Turroni, Erasmo Neviani, Ilario Ferrocino, Raffaella Di Cagno, Luca Ruini, Roberto Ciati, Donato Angelino, Jane Maddock, Marco Gobbetti, Furio Brighenti, Daniele Del Rio, Francesca Scazzina
Food and beverage consumption has a great impact on the environment, although there is a lack of information concerning the whole diet. The environmental impact of 153 Italian adults (51 omnivores, 51 ovo-lacto-vegetarians, 51 vegans) and the inter-individual variability within dietary groups were assessed in a real-life context. Food intake was monitored with a 7-d dietary record to calculate nutritional values and environmental impacts (carbon, water, and ecological footprints). The Italian Mediterranean Index was used to evaluate the nutritional quality of each diet...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733576/mait-cells-accumulate-in-placental-intervillous-space-and-display-a-highly-cytotoxic-phenotype-upon-bacterial-stimulation
#9
Martin Solders, Laia Gorchs, Tom Erkers, Anna-Carin Lundell, Silvia Nava, Sebastian Gidlöf, Eleonor Tiblad, Isabelle Magalhaes, Helen Kaipe
During pregnancy, the maternal immune system must tolerate the developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on the MR1 molecule, but their presence and function at the foetal-maternal interface is not known. We here isolated mononuclear cells from paired samples of peripheral blood (PB), intervillous blood (IVB), and decidua parietalis (DP) following uncomplicated term pregnancies...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733520/the-ketogenic-diet-is-not-feasible-as-a-therapy-in-a-cd-1-nu-nu-mouse-model-of-renal-cell-carcinoma-with-features-of-stauffer-s-syndrome
#10
Silvia Vidali, Sepideh Aminzadeh-Gohari, René Günther Feichtinger, Renaud Vatrinet, Andreas Koller, Felix Locker, Tricia Rutherford, Maura O'Donnell, Andrea Stöger-Kleiber, Bridget Lambert, Thomas Klaus Felder, Wolfgang Sperl, Barbara Kofler
The ketogenic diet (KD), a high-fat low-carbohydrate diet, has shown some efficacy in the treatment of certain types of tumors such as brain tumors and neuroblastoma. These tumors are characterized by the Warburg effect. Because renal cell carcinoma (RCC) presents similar energetic features as neuroblastoma, KD might also be effective in the treatment of RCC. To test this, we established xenografts with RCC 786-O cells in CD-1 nu/nu mice and then randomized them to a control diet or to KDs with different triglyceride contents...
July 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28733467/acromegaly-at-diagnosis-in-3173-patients-from-the-li%C3%A3-ge-acromegaly-survey-las-database
#11
Patrick Petrossians, Adrian F Daly, Emil Natchev, Luigi Maione, Karin Blijdorp, Mouna Sahnoun Fathallah, Renata Auriemma, Alpha M Diallo, Anna-Lena Hulting, Diego Ferone, Vaclav Hana, Silvia Filipponi, Caroline Sievers, Claudia Nogueira, Carmen Fajardo Montañana, Davide M C Carvalho, Vaclav Hana, Günter K Stalla, Marie-Lise Jaffrain-Réa, Brigitte Delemer, Annamaria A L Colao, Thierry Brue, Sebastian J C M M Neggers, Sabina Zacharieva, Philippe Chanson, Albert Beckers
Acromegaly is a rare disorder caused by chronic growth hormone (GH) hypersecretion. While diagnostic and therapeutic methods have advanced, little information exists on trends in acromegaly characteristics over time. The Liège Acromegaly Survey (LAS) database, a relational database, is designed to assess the profile of acromegaly patients at diagnosis and during long-term follow-up at multiple treatment centers. The study population consisted of 3173 acromegaly patients from ten countries; 54.5% were female...
July 21, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/28733272/adherence-to-web-based-self-assessments-in-long-term-direct-to-patient-research-two-year-study-of-multiple-sclerosis-patients
#12
Peter Joseph Jongen, Ingrid E H Kremer, Elena Hristodorova, Silvia M A A Evers, Anton Kool, Esther M van Noort, Mickaël Hiligsmann
BACKGROUND: Direct-to-patient research via Web-based questionnaires is increasingly being used. Missed data or delayed reporting of data may negatively affect the quality of study results. It is insufficiently known to what degree patients adhere to agreed self-assessment schedule over the long term and whether questionnaires are filled out in a timely manner. OBJECTIVE: The objective of this study was to investigate patients' adherence to a self-assessment schedule with low-frequency long questionnaires versus that with a high-frequency short questionnaire...
July 21, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28733220/pan-mtor-inhibitor-mln0128-is-effective-against-intrahepatic-cholangiocarcinoma-induced-in-mice-by-akt-and-yap-co-expression
#13
Shanshan Zhang, Xinhua Song, Dan Cao, Zhong Xu, Biao Fan, Li Che, Junjie Hu, Bin Chen, Mingjie Dong, Maria G Pilo, Antonio Cigliano, Katja Evert, Silvia Ribback, Frank Dombrowski, Rosa M Pascale, Antonio Cossu, Gianpaolo Vidili, Alberto Porcu, Maria M Simile, Giovanni M Pes, Gianluigi Giannelli, John Gordan, Lixin Wei, Matthias Evert, Wenming Cong, Diego F Calvisi, Xin Chen
BACKGROUND & AIMS: Intrahepatic cholangiocarcinoma (ICC) is a lethal malignancy without effective treatment options. MLN0128, a second-generation pan-mTOR inhibitor, shows efficacy for multiple tumor types. METHODS: We established a novel ICC mouse model via hydrodynamic transfection of activated forms of AKT (myr-AKT) and Yap (YapS127A) protooncogenes (that will be referred to as AKT/YapS127A). Genetic approaches were applied to study the requirement of mTORC1 and mTORC2 in mediating AKT/YapS127A driven tumorigenesis...
July 18, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28733123/augmentative-and-alternative-communication-effects-on-quality-of-life-in-patients-with-locked-in-syndrome-and-their-caregivers
#14
Francesco Corallo, Lilla Bonanno, Viviana Lo Buono, Simona De Salvo, Carmela Rifici, Patrizia Pollicino, Cettina Allone, Rosanna Palmeri, Antonino Todaro, Antonella Alagna, Alessia Bramanti, Placido Bramanti, Silvia Marino
BACKGROUND: The aim of this study was to evaluate the impact of the augmentative and alternative communication system on anxiety and depression performances and the quality of life (QoL) of patients who have the locked-in syndrome (LIS) and that of their caregivers. METHOD: We enrolled 15 patients and their principal caregivers. The assessment consisted of the administration of the employed Short Form-36 Questionnaire for the assessment of QoL, the Hamilton Anxiety Rating Scale (HAM-A) for anxiety, and the Beck Depression Inventory for depression...
July 18, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28732398/simulation-of-targeted-pollutant-mitigation-strategies-to-reduce-nitrate-and-sediment-hotspots-in-agricultural-watershed
#15
Awoke Dagnew Teshager, Philip W Gassman, Silvia Secchi, Justin T Schoof
About 50% of U.S. water pollution problems are caused by non-point source (NPS) pollution, primarily sediment and nutrients from agricultural areas, despite the widespread implementation of agricultural Best Management Practices (BMPs). However, the effectiveness of implementation strategies and type of BMPs at watershed scale are still not well understood. In this study, the Soil and Water Assessment Tool (SWAT) ecohydrological model was used to assess the effectiveness of pollutant mitigation strategies in the Raccoon River watershed (RRW) in west-central Iowa, USA...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28731721/enantioselective-synthesis-of-spiro-indolizidine-1-3-oxindoles
#16
Maria Pérez, Carlos Ramos, Lucia Massi, Silvia Gazzola, Chiara Taglienti, Nihan Yayik, Elies Molins, Antonio Viayna, F Javier Luque, Joan Bosch, Mercedes Amat
A three-step procedure for the enantioselective synthesis of spiro[indolizidine-1,3'-oxindoles], consisting of a stereoselective cyclocondensation reaction between (S)-tryptophanol and a prochiral or racemic δ-oxoester, bromination of the resulting oxazolopiperidone lactam, and a final stereoselective spirocyclization, is reported.
July 21, 2017: Organic Letters
https://www.readbyqxmd.com/read/28731654/validation-of-the-hematopoietic-cell-transplantation-specific-comorbidity-index-in-a-retrospective-cohort-of-children-and-adolescents-who-received-an-allogeneic-transplantation-in-argentina
#17
Carlos M Figueroa Turienzo, Carolina Cernadas, Mariana Roizen, Silvia Pizzi, Raquel Staciuk
INTRODUCTION: Hematopoietic cell transplantationis a therapy with a risk of transplant-related mortality (TRM), which may vary depending on prior comorbidities. The Hematopoietic Cell Transplantation-Specific Comorbidity Index (HCT-CI) is an instrument developed to measure this risk. There are very few reports on its use in pediatrics. The objective of this study was to validate the HCT-CI in a pediatric cohort of allogeneic hematopoietic-cell transplantation recipients in Argentina. POPULATION AND METHODS: Retrospective cohort made up of 140 transplant patients at Hospital J...
August 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28731233/integration-of-cellular-and-molecular-endpoints-to-assess-the-toxicity-of-polycyclic-aromatic-hydrocarbons-in-hepg2-cell-line
#18
Patrícia I Morgado, Sílvia Jose, Riccardo Wanke, Alexandra M M Antunes, Ana Sofia Cardoso, Luisa Jordao
Polycyclic aromatic hydrocarbons (PAHs) are persistent pollutants present in the environment with known mutagenic and carcinogenic properties. In the present study the effect of exposure to single or multiple doses of benzo[a]anthracene (BaA), pyrene (Pyr) and three halogenated derivatives of these compounds (1-ClPyr; 1-BrPyr and 7-ClBaA) were evaluated in a liver-derived human cell line (HepG2). Cytotoxicity as assessed by the classic MTT and neutral red assays showed a mild toxic effect in response to single or multiple dose exposure for up to 72h; except for multiple dose exposure to BaA and 7-ClBaA (1μM per day during 4 days) and single exposure to 10 μM BaA...
July 21, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28731075/a-simple-supramolecular-assay-for-drug-detection-in-urine
#19
Silvia Sonzini, Jade A McCune, Peter Ravn, Oren A Scherman, Christopher F van der Walle
A supramolecular colorimetric assay utilising the macrocyclic host cucurbit[7]uril (CB[7]) with a commercial dye molecule, neutral red (NR), was evaluated as a novel method for drug detection in urine of a model therapeutic peptide drug Octreotide.
July 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28730964/ccl2-is-modulated-by-cytokines-and-ppar-g-in-anaplastic-thyroid-cancer
#20
Silvia Martina Ferrari, Giusy Elia, Simona Piaggi, Enke Baldini, Salvatore Ulisse, Mario Miccoli, Gabriele Materazzi, Alessandro Antonelli, Poupak Fallahi
BACKGROUND AND OBJECTIVE: Chemokine (C-C motif) ligand (CCL)2, the prototype Th2 chemokine, is secreted by tumor cells, and has growth promoting effects. Whether CCL2 protumorigenic activities will be validated, then CCL2 and its receptor CCR2 may be therapeutic targets in cancer. METHODS: We tested in "primary human anaplastic thyroid carcinoma (ATC) cells" (ANA) versus "normal thyroid follicular cells" (TFC): a) CCL2 secretion basally, after IFN-g and/or TNF-a stimulation; b) PPARg activation by thiazolidinediones (TZDs), rosiglitazone or pioglitazone, on CCL2 secretion, and on proliferation and apoptosis in ANA...
July 19, 2017: Anti-cancer Agents in Medicinal Chemistry
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