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Longbin Zheng, Ying Li, Xuesong Li, Jiayuan Kou, Zhaoyu Zhong, Yueqing Jiang, Zhongni Liu, Ye Tian, Liming Yang
BACKGROUND/AIMS: Sonodynamic therapy (SDT) is considered a new approach for the treatment of atherosclerosis. We previously confirmed that hydroxyl acetylated curcumin (HAC) was a sonosensitizer. In this study, we investigated the mechanism of THP-1 macrophage apoptosis and autophagy induced by HAC mediated SDT (HAC-SDT). METHODS: Cell viability was measured using a CCK-8 assay. Laser scanning confocal microscopy was used to measure the levels of intracellular reactive oxygen species (ROS), sub-cellular HAC localization, BAX and cytochrome C translocation, LC3 expression, monodansylcadaverine staining and Dil-labeled oxidized low density lipoprotein (Dil-ox-LDL) uptake...
October 17, 2016: Cellular Physiology and Biochemistry
Wei Li, Qing Yang, Zhaoyu Gu, Chuanbao Wu, Dong Meng, Jie Yu, Qiuju Chen, Yang Li, Hui Yuan, Dongmei Wang, Tianzhong Li
In this study, we characterized a naturally occurring self-compatible apple cultivar, 'CAU-1' (S1S9), and studied the underlying mechanism that causes its compatibility. Analyses of both fruit set rate and seed number after self-pollination or cross-pollination with 'Fuji' (S1S9), and of pollen tube growth, demonstrated that 'CAU-1' is self-compatible. Genetic analysis by S-RNase PCR-typing of selfed progeny of 'CAU-1' revealed the presence of all progeny classes (S1S1, S1S9, and S9S9). Moreover, no evidence of S-allele duplication was found...
November 2016: Plant Science: An International Journal of Experimental Plant Biology
Fumin Pan, Bin Shen, Surendra K Chy, Zhiyao Yong, Xiaoming Liu, Zhaoyu Ba, Zhonghan Liu, Jianguang Zhu, Desheng Wu
OBJECTIVE: To investigate the clinical outcomes of transforaminal endoscopic system(TESSYS) for discogenic low back pain(DLBP). METHODS: 62 consecutive patients with one-level DLBP applying to the including criteria underwent TESSYS from January 2010 to December 2013. These patients included 38 males and 24 females with a mean age of 42.7 ± 5.4 years (range, 18-73 yr) and a mean follow-up of 26.8 ± 4.2 months (range, 12-48 months). The visual analog scale (VAS) were used for back pain, the Oswestry Disability Index (ODI) for lumbar function, and the modified MacNab criteria for clinical global outcomes...
September 28, 2016: International Journal of Surgery
Huimin Gao, Zhaoyu Chen, Yongmei Fu, Xiaoyan Yang, Ruihui Weng, Rui Wang, Jianjun Lu, Mengqiu Pan, Kunlin Jin, Chris McElroy, Beisha Tang, Ying Xia, Qing Wang
The nuclear orphan receptor, Nur77 plays important roles in neuroimflammation, apoptosis, and dopaminergic neurodegeneration. We conducted a further mechanistic investigation into the association of Nur77 with cell death. Cytosporone B (Csn-B), an agonist for Nur77, and Nur77 knockdown were adopted in the 6-hydroxydopamine (OHDA)-lesioned PC12 cells to investigate the mechanisms underlying Nur77-mediated injury. The 6-OHDA incubation caused Nur77 translocation from the nucleus to cytosol and Endoplasm reticulum (ER) and induced co-localization of Tom20/Nur77 and Protein Disulfide Isomerase (PDI)/Nur77...
September 29, 2016: Scientific Reports
Zhaoyu Wang, Yanhong Bi, Rongling Yang, Xiangjie Zhao, Ling Jiang, Chun Zhu, Yuping Zhao, Jianbo Jia
Efficient and highly regioselective synthesis of the potential 6''-O-sorboyl-polydatin prodrug in biomass-derived 2-methyltetrahydrofuran (2-MeTHF) was achieved using Candida antarctica lipase B for the first time. Under the optimal conditions, the initial reaction rate, maximum substrate conversion, and 6''-regioselectivity were as high as 8.65 mM/h, 100%, and 100%, respectively. Kinetic and operational stability investigations evidently demonstrated excellent enzyme compatibility of the 2-MeTHF compared to the traditional organic solvents...
2016: BioMed Research International
Xaoyan Dai, Ye Ding, Zhaoyu Liu, Wencheng Zhang, Ming-Hui Zou
RATIONALE: Elevated levels of C/EBP homologous protein (CHOP), a member of the C/EBP transcription factor family, in advanced atherosclerotic plaques is reported to be associated with atherosclerotic plaque rupture in humans. However, the molecular mechanism by which CHOP accumulation occurs is poorly defined. OBJECTIVE: The aim of this study was to investigate if (1) macrophage AMP-activated kinase (AMPK) regulates cellular CHOP accumulation and (2) whole-body Ampk deletion leads to neointimal disruption...
September 20, 2016: Circulation Research
Xiaobo Wei, Ronghua Yan, Zhaoyu Chen, Ruihui Weng, Xu Liu, Huimin Gao, Xiaofeng Xu, Zhuang Kang, Zhexing Liu, Yan Guo, Zhenhua Liu, Jan Petter Larsen, Jin Wang, Beisha Tang, Mark Hallett, Qing Wang
This study aimed to identify a PD-specific MRI pattern using combined diffusion tensor imaging (DTI) and arterial spin labeling (ASL) to discriminate patients with early PD from healthy subjects and evaluate disease status. Twenty-one early and 22 mid-late PD patients, and 22 healthy, age/gender-matched controls underwent 3-T MRI with apparent diffusion coefficient (ADC), fractional anisotropy (FA), fiber number (FN) and cerebral blood flow (CBF) measurements. We found that compared with healthy subjects, there was a profound reduction in FN passing through the SN in PD...
2016: Scientific Reports
Zhifa Wang, Zhaoyu Ju, Longlong He, Zhijin Li, Yanpu Liu, Bin Liu
PURPOSE: In a previous study, the authors verified the protective efficacy of adipose-derived stem cells (ADSCs) on the prevention of salivary gland (SG) damage induced by irradiation in mice. As a critical step before implementation in clinical practice, the present study investigated the protective effect of ADSCs in a miniature pig SG model, because miniature pigs share many characteristics with humans. MATERIALS AND METHODS: Third-passage autologous ADSCs at a concentration of 4 × 10(6) cells/mL were transplanted by intraglandular injection into parotid glands (PGs) immediately after local irradiation at a single dose of 20 Gy...
August 16, 2016: Journal of Oral and Maxillofacial Surgery
Guolin Zhao, Ting Zhao, Huifu Xiao, Zilong Liu, Guipeng Liu, Jianhong Yang, Zhaoyu Ren, Jintao Bai, Yonghui Tian
We experimentally demonstrate a tunable Fano resonance which originates from the optical interference between two different resonant cavities using silicon micro-ring resonator with feedback coupled waveguide fabricated on silicon-on-insulator (SOI) substrate. The resonance spectrum can be periodically tuned via changing the resonant wavelengths of two resonators through the thermo-optic effect. In addition to this, we can also change the transmission loss of the feedback coupled waveguide (FCW) to tune the resonance spectrum by the injection free carriers to FCW...
September 5, 2016: Optics Express
Zheng Wang, Guangyan Miao, Xue Xue, Xiangyang Guo, Chongzhen Yuan, Zhaoyu Wang, Gangming Zhang, Yingyu Chen, Du Feng, Junjie Hu, Hong Zhang
Mutations in the human autophagy gene EPG5 cause the multisystem disorder Vici syndrome. Here we demonstrated that EPG5 is a Rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes. EPG5 is recruited to late endosomes/lysosomes by direct interaction with Rab7 and the late endosomal/lysosomal R-SNARE VAMP7/8. EPG5 also binds to LC3/LGG-1 (mammalian and C. elegans Atg8 homolog, respectively) and to assembled STX17-SNAP29 Qabc SNARE complexes on autophagosomes. EPG5 stabilizes and facilitates the assembly of STX17-SNAP29-VAMP7/8 trans-SNARE complexes, and promotes STX17-SNAP29-VAMP7-mediated fusion of reconstituted proteoliposomes...
September 1, 2016: Molecular Cell
Kejia Wen, Lijuan Yang, Tuanlin Xiong, Chao Di, Danhui Ma, Menghua Wu, Zhaoyu Xue, Xuedi Zhang, Li Long, Weimin Zhang, Jiaying Zhang, Xiaolin Bi, Junbiao Dai, Qiangfeng Zhang, Zhi John Lu, Guanjun Gao
Long noncoding RNAs (lncRNAs), a recently discovered class of cellular RNAs, play important roles in the regulation of many cellular developmental processes. Although lncRNAs have been systematically identified in various systems, most of them have not been functionally characterized in vivo in animal models. In this study, we identified 128 testis-specific Drosophila lncRNAs and knocked out 105 of them using an optimized three-component CRISPR/Cas9 system. Among the lncRNA knockouts, 33 (31%) exhibited a partial or complete loss of male fertility, accompanied by visual developmental defects in late spermatogenesis...
September 2016: Genome Research
Zhaoyue Wang, Li Sheng, Yue Yang, Fan Yang, Xiao Xiao, Jing Hua, Canjie Guo, Yiran Wei, Ruqi Tang, Qi Miao, Jun Zhang, Yanmei Li, Jingyuan Fang, Dekai Qiu, Edward L Krawitt, Christopher L Bowlus, M Eric Gershwin, Qixia Wang, Xiong Ma
There is a paucity of information related to the usefulness of corticosteroid therapy in autoimmune hepatitis (AIH) with decompensated cirrhosis. In this study, we sought to determine the therapeutic effect of corticosteroids in this special group of AIH patients. Eighty-two AIH patients with decompensated cirrhosis were included through a retrospective analysis from January 2009 to September 2015. Sixty-four patients were treated with corticosteroids while 18 patients did not receive any corticosteroids. Clinical, laboratory, and histological characteristics and outcomes were analyzed comparing corticosteroid-treated and untreated groups...
August 11, 2016: Clinical Reviews in Allergy & Immunology
Meng Zhou, Lei Zhong, Wanying Xu, Yifan Sun, Zhaoyue Zhang, Hengqiang Zhao, Lei Yang, Jie Sun
Deregulation of long non-coding RNAs (lncRNAs) expression has been proven to be involved in the development and progression of cancer. However, expression pattern and prognostic value of lncRNAs in breast cancer recurrence remain unclear. Here, we analyzed lncRNA expression profiles of breast cancer patients who did or did not develop recurrence by repurposing existing microarray datasets from the Gene Expression Omnibus database, and identified 12 differentially expressed lncRNAs that were closely associated with tumor recurrence of breast cancer patients...
2016: Scientific Reports
Yanxian Hu, Zhaoyu Wang, Xin Huang, Bijun Xia, Lan Tang, Zhijie Zheng, Ling Ye
1. Gelsemium elegans Benth (Loganiaceae) is a toxic plant that can be used for committing suicide besides alleviating pains. Its anti-inflammatory and analgesic effect mainly come from its active ingredient, namely koumine. Koumine, an indole alkaloid, possesses widely pharmacological effects especially inhibition of neuropathic pain. 2. This study aimed to investigate the metabolic profile of koumine using human liver microsomes (HLMs), selective chemical inhibitors and recombinant human CYP isoforms. Ultra-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS) was used to detect and identify metabolites...
August 8, 2016: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Chuanfang Chen, Shunhao Wang, Linlin Li, Pingping Wang, Changyou Chen, Zhaoyu Sun, Tao Song
The bacterial magnetic nanoparticles (BMPs) are biomineralized by the magnetotactic bacteria and naturally covered with a layer of biomembrane. Herein, BMPs were isolated and firstly used for the photothermal therapy (PTT) of cancer under the guidance of magnetic resonance imaging (MRI) in vitro and in vivo. The results showed that BMPs could rapidly convert the energy of 808 nm near-infrared (NIR) light into heat. After internalization by the HepG2 tumor cells, BMPs with good biocompatibility could induce an efficient killing effect after NIR light irradiation, along with a change of mitochondrial membrane potential (ΔΨm) and level of intracellular reactive oxygen species (ROS)...
October 2016: Biomaterials
Danli Kong, Ying Zhan, Zhaoyu Liu, Ting Ding, Min Li, Haibing Yu, Laxi Zhang, Huawen Li, Aiyue Luo, Dongwei Zhang, Yifei Wang, Shixuan Wang, Zhefan Zhang, Hongyu Zhang, Xiaodong Huang, Paul Yao, Yuanling Ding, Zhengxiang Liu
SIRT1 has many important molecular functions in aging, and the estrogen receptors (ERs) have a vasculoprotective effect, although the detailed mechanism for the roles of SIRT1 and ERs in vascular aging remains unclear. We found that ERβ expression in the endothelium was reduced in aging mice, and the expression of ERα and SIRT1 did not change, while SIRT1 activity declined. Further investigation showed that the ERβ expression was regulated by SIRT1 through complexes of SIRT1-PPARγ/RXR-p300 that bind to a PPRE (PPAR response element) site on the ERβ promoter, and the declined SIRT1 function in aging mice was due to compromised phosphorylation at S154...
July 29, 2016: Aging Cell
Lianxin Hu, Jiajun Xu, Meng-Xin Yin, Liguo Zhang, Yi Lu, Wenqing Wu, Zhaoyu Xue, Margaret S Ho, Guanjun Gao, Yun Zhao, Lei Zhang
Non-receptor tyrosine kinase activated cdc42 kinase was reported to participate in several types of cancers in mammals. It is also believed to have an anti-apoptotic function in Drosophila. Here, we report the identification of Drosophila activated cdc42 kinase as a growth promoter and a novel Hippo signaling pathway regulator. We find that activated cdc42 kinase promotes tissue growth through modulating Yorkie activity. Furthermore, we demonstrate that activated cdc42 kinase interacts with Expanded and induces tyrosine phosphorylation of Expanded on multiple sites...
2016: Cell Discovery
Huaiping Zhu, Miao Zhang, Zhaoyu Liu, Junjie Xing, Cate Moriasi, Xiaoyan Dai, Ming-Hui Zou
OBJECTIVE: AMP-activated protein kinase (AMPK), an energy and redox sensor, is activated in response to various cellular stresses, including hypoxia, nutrient deprivation, oxidative stress, and fluid shear stress at the site of vessel blockade. The activation of AMPK is involved in angiogenesis. However, it is unknown whether AMPK can influence arteriogenesis. Here, we demonstrate the contribution of macrophage AMPK to arteriogenesis and collateral remodeling and their underlying mechanisms in well-characterized in vivo and in vitro models...
September 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
Shujun Jiang, Qinghua Wang, Zan Huang, Anying Song, Yu Peng, Siyuan Hou, Shiying Guo, Weiyun Zhu, Sheng Yan, Zhaoyu Lin, Xiang Gao
Duodenum-jejunum gastric bypass (DJB) has been used to treat morbid diabetic patients. However, neither the suitability among patients nor the mechanisms of this surgical treatment is clear. Previously, we reported a new mouse strain named Timo as type 2 diabetes model caused by brain-derived neurotrophic factor (Bdnf) deficiency. In this study, we found that DJB on Timo mice reversed their metabolic abnormalities without altering the expression of Bdnf. Glucose tolerance and insulin sensitivity were improved greatly, along with reduction of fat accumulation in liver and white adipose tissue...
August 2016: American Journal of Pathology
Zhaoyu Hu, Aileen Bongat, Laurence A Mulard
Protection against bacterial infections, including shigellosis, can be achieved by antibodies against the bacterial surface polysaccharide. In line with our efforts to develop vaccine candidates for shigellosis, we report the synthesis of penta-, deca-, and pentadecasaccharides as well as of tri-, octa-, and tridecasaccharides, as endchain and intrachain fragments, respectively, of the surface polysaccharide of Shigella flexneri 3a, a prevalent serotype. The syntheses rely on the efficiency of the trichloroacetimidate glycosylation chemistry, whereby iteration with di- and trisaccharide building blocks provided fragments made of up to three mono-O-acetylated polysaccharide repeating units...
July 7, 2016: Chemistry, An Asian Journal
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