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https://www.readbyqxmd.com/read/28803837/silencing-of-glut1-induces-chemoresistance-via-modulation-of-akt-gsk-3%C3%AE-%C3%AE-catenin-survivin-signaling-pathway-in-breast-cancer-cells
#1
Sunhwa Oh, KeeSoo Nam, Hyungjoo Kim, Incheol Shin
Cancer cells require increased aerobic glycolysis to support rapid cell proliferation. For their increased energy demands, cancer cells express glucose transporter (Glut) proteins at a high level. Glut1 is associated with basal-like breast cancer and is considered a potential therapeutic target. To investigate the possibility of Glut1 as a therapeutic target in breast cancer cells, we downregulated Glut1 in triple-negative breast cancer (TNBC) cell lines using a short hairpin system. We determined whether Glut1 silencing might enhance anti-proliferative effect of chemotherapeutic agents...
August 10, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28803777/paraoxonase-2-facilitates-pancreatic-cancer-growth-and-metastasis-by-stimulating-glut1-mediated-glucose-transport
#2
Arvindhan Nagarajan, Shaillay Kumar Dogra, Lisha Sun, Neeru Gandotra, Thuy Ho, Guoping Cai, Gary Cline, Priti Kumar, Robert A Cowles, Narendra Wajapeyee
Metabolic deregulation is a hallmark of human cancers, and the glycolytic and glutamine metabolism pathways were shown to be deregulated in pancreatic ductal adenocarcinoma (PDAC). To identify new metabolic regulators of PDAC tumor growth and metastasis, we systematically knocked down metabolic genes that were overexpressed in human PDAC tumor samples using short hairpin RNAs. We found that p53 transcriptionally represses paraoxonase 2 (PON2), which regulates GLUT1-mediated glucose transport via stomatin. The loss of PON2 initiates the cellular starvation response and activates AMP-activated protein kinase (AMPK)...
July 21, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28803235/18f-fdg-uptake-in-well-differentiated-neuroendocrine-tumors-correlates-with-both-ki-67-and-vhl-pathway-inactivation
#3
Margot Bucau, Astrid Laurent-Bellue, Nicolas Poté, Olivia Hentic, Jérôme Cros, Nidaa Mikail, Vinciane Rebours, Philippe Ruszniewski, Rachida Lebtahi, Anne Couvelard
<br>Introduction: 18FDG PET-scanner positivity correlates with poor prognosis in neuroendocrine neoplasms (NEN). Glucose transporter 1 (GLUT1) and carbonic anhydrase 9 (CA9) are markers of agressivity in tumors. Together with pVHL, they are involved in tumor cell metabolism via the hypoxia-inducible factor (HIF) signaling pathway. The aim of this study was to compare, in a series of well-differentiated neuroendocrine tumors (NET), the 18-FDG uptake and expression of proliferation marker Ki-67, GLUT-1, CA9 and pVHL...
August 11, 2017: Neuroendocrinology
https://www.readbyqxmd.com/read/28799035/effect-of-heat-stress-on-protein-utilization-and-nutrient-transporters-in-meat-type-chickens
#4
Walid S Habashy, Marie C Milfort, Alberta L Fuller, Youssef A Attia, Romdhane Rekaya, Samuel E Aggrey
The aim of this study was to investigate the effect of heat stress (HS) on digestibility of protein and fat and the expression of nutrient transporters in broilers. Forty-eight male Cobb500 chicks were used in this study. At day 14, birds were randomly divided into two groups and kept under either constant normal temperature (25 °C) or high temperature (35 °C) in individual cages. Five birds per treatment at 1 and 12 days post-treatment were euthanized, and Pectoralis major (P. major) and ileum were sampled for gene expression analysis...
August 10, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28794109/comparative-study-on-glucose-transporters-expression-and-activity-between-stem-cell-derived-brain-microvascular-endothelial-cells-and-hcmec-d3-cells
#5
Abraham J Al-Alahmad
Glucose constitutes the major source of energy of mammalian brains. Glucose uptake at the blood-brain barrier (BBB) occurs through a facilitated glucose transport, through glucose transporter 1 (GLUT1), although other isoforms have been described at the BBB. Mutations in GLUT1 is associated with the GLUT1 deficiency syndrome (GLUT1DS), yet none of the current in vitro models of the human BBB maybe suited for modelling such disorder. In this study, we investigated the expression of glucose transporters and glucose diffusion across the human BBB using brain microvascular endothelial cells (BMECs) derived from healthy patient-derived induced pluripotent stem cells (iPSCs)...
August 9, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28793782/direct-activation-of-nadph-oxidase-2-by-2-deoxyribose-1-phosphate-triggers-nuclear-factor-kappa-b-dependent-angiogenesis
#6
Dina Vara, Joanna M Watt, Tiago M Fortunato, Harry Mellor, Matthew Burgess, Kate Wicks, Kimberly Mace, Shaun Reeksting, Anneke T Lubben, Caroline Pd Wheeler-Jones, Giordano Pula
AIMS: Deoxyribose-1-phosphate (dRP) is a pro-angiogenic paracrine stimulus released by cancer cells, platelets and macrophages and acting on endothelial cells. The objective of this study was to clarify how dRP stimulates angiogenic responses in human endothelial cells. RESULTS: Live cell imaging, electron paramagnetic resonance (EPR), pull-down of dRP-interacting proteins followed by immunoblotting, gene silencing of different NOXs and their regulatory co-subunits by siRNA transfection, and experiments with inhibitors of the sugar transporter GLUT1 were utilized to demonstrate that dRP acts intracellularly by directly activating the endothelial NADPH oxidase 2 (NOX2) complex, but not NOX4...
August 10, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28791350/prl-3-improves-colorectal-cancer-cell-proliferation-and-invasion-through-il-8-mediated-glycolysis-metabolism
#7
Heyang Xu, Yujie Zeng, Lu Liu, Qian Gao, Shaowen Jin, Qiusheng Lan, Wei Lai, Xingxi Luo, Heng Wu, Yongliang Huang, Zhonghua Chu
Phosphatase of regenerating liver-3 (PRL-3) has been found to be overexpressed in liver metastases of colorectal cancer and rarely expressed in primary tumors, which plays an important role in the metastasis of colorectal cancer cells. Metabolism reprogramming has been found to be a hallmark of cancer cells, and aerobic glycolysis is a metabolic adaption for cancer cells and promotes cell proliferation. However, the association between PRL-3 and glycolysis in colorectal cancer cells is not well understood. In the present study, we explored the association between PRL-3 and glycolysis...
August 2, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28778020/long-term-effect-of-dietary-overload-lithium-on-the-glucose-metabolism-in-broiler-chickens
#8
Shiping Bai, Shuqin Pan, Keying Zhang, Xuemei Ding, Jianping Wang, Qiufeng Zeng, Yue Xuan, Zuowei Su
Lithium, like insulin, activates glycogen synthase and stimulates glucose transport in rat adipocytes. To investigate the effect of dietary overload lithium on glucose metabolism in broiler chickens, one-day-old chicks were fed a basal diet supplemented with 0 (control) or 100mg lithium/kg (overload lithium) for 35days. Compared to controls, glucose disappearance rates were lower (p=0.035) 15-120min after glucose gavage, and blood glucose concentrations were lower (p=0.038) 30min after insulin injection in overload lithium broilers...
July 22, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28768434/metabolic-reprogramming-during-hepatitis-b-disease-progression-offers-novel-diagnostic-and-therapeutic-opportunities
#9
REVIEW
Jesse Jr Masson, Hugh Ww Billings, Clovis S Palmer
Metabolic remodeling occurs in immune cells during an infection. Host cells must upregulate energy production for growth, proliferation, and effector functions to limit the damage imposed by pathogens. One example, the hepatitis B virus, induces hepatic injury in human hepatocytes through dysregulation of aerobic glycolysis and lipid metabolism. Increased glycolytic metabolism mediated by elevated expression of Glut1, glucose influx, and lactate secretion is associated with this Warburg phenotype, a classic metabolic signature also observed in cancer cells...
August 2017: Antiviral Chemistry & Chemotherapy
https://www.readbyqxmd.com/read/28764171/evaluation-of-hypoxia-inducible-factor-1%C3%AE-and-glucose-transporter-1-expression-in-non-melanoma-skin-cancer-an-immunohistochemical-study
#10
Iman Seleit, Ola Ahmed Bakry, Dalia Rifaat Al-Sharaky, Rania Abdel Aziz Ragab, Shimaa Ahmed Al-Shiemy
INTRODUCTION: Hypoxia Inducible Factor-1 (HIF-1) is a mediator enabling cell adaptation to hypoxia. It plays its role mainly through transcription of many target genes including Glucose Transporter-1 (GLUT-1) gene. AIM: The present work aimed at evaluating the pattern and distribution of HIF-1α and GLUT-1 in each case and control. MATERIALS AND METHODS: A case-control and retrospective study was conducted on archival blocks diagnosed from pathology department as, Basal Cell Carcinoma (BCC, 20 cases), cutaneous Squamous Cell Carcinoma (SCC, 20 cases) and 20 normal site-matched skin biopsies from age and gender-matched healthy subjects as a control...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28760661/sox4-promotes-proliferative-signals-by-regulating-glycolysis-through-akt-activation-in-melanoma-cells
#11
Wei Dai, Xinyuan Xu, Shuli Li, Jingjing Ma, Qiong Shi, Sen Guo, Lin Liu, Weinan Guo, Peng Xu, Yuanmin He, Guannan Zhu, Liwen Wang, Rui Ge, Yu Liu, Zhe Jian, Gang Wang, Lan Shen, Tianwen Gao, Chunying Li
The sex-determining region Y-related high-mobility-group box transcription factor 4 (SOX4) plays a fundamental role during embryogenesis and controls cell fate and differentiation. Recently, increased SOX4 expression has been reported in various cancer types, contributing to the progression and survival of cancer cells. However, the distinct functions and downstream targets of SOX4 remain to be fully elucidated. In this study, we initially found elevated SOX4 expression in melanoma. SOX4 regulates apoptosis and cell cycle arrest, affects glucose consumption and lactate production, and consequently, promotes melanoma cell proliferation...
July 28, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28760221/approaching-to-dm2-through-sodium-glucose-cotransporter-2-does-it-make-sense
#12
Julián Segura
The kidney is involved in glucose homeostasis through three main mechanisms: renal gluconeogenesis, renal glucose consumption and glucose reabsorption in the proximal tubule. Glucose reabsorption is one of the most relevant physiological functions of the kidney, through which filtered glucose is fully recovered, urine is free of glucose, and calorie loss is prevented. Approximately 90% of the glucose is reabsorbed in the S1 segment of the proximal tubule, where GLUT2 and SGLT2 transporters are located, while the remaining 10% is reabsorbed in the S3 segment by SGLT1 and GLUT1 transporters...
November 2016: Medicina Clínica
https://www.readbyqxmd.com/read/28759035/sall4-promotes-glycolysis-and-chromatin-remodeling-via-modulating-hp1%C3%AE-glut1-pathway
#13
J Kim, S Xu, L Xiong, L Yu, X Fu, Y Xu
SALL4 has recently been identified to promote chemo-resistance in multiple types of cancer, but the underlying mechanism remains to be fully established. Open chromatin structure is important for DNA damage response (DDR) and DNA repair. Here, we demonstrate that SALL4 promotes open chromatin by destabilizing heterochromatin protein 1α (HP1α) by recruiting ubiquitin E3 ligase CUL4B to HP1α. The silencing of SALL4 in cancer cells decreased the expression levels of Glut1 and inhibited glycolysis in cancer cells...
July 31, 2017: Oncogene
https://www.readbyqxmd.com/read/28756482/epr-oximetry-of-cetuximab-treated-head-and-neck-tumours-in-a-mouse-model
#14
H Gustafsson, A Kale, A Dasu, A Lund, P-H Edqvist, K Roberg
Head and neck squamous cell carcinoma (HNSCC) tumours are associated with high mortality despite advances in therapy. The monoclonal antibody cetuximab (Erbitux(®)) has been approved for the treatment of advanced HNSCC. However, only a subset of HNSC patients receiving cetuximab actually responds to treatment, underlining the need for a means to tailor treatments of individual patients. The aim of the present study was to investigate the effect of cetuximab treatment on tumour growth, on tumour partial oxygen pressure as measured by LiPc electron paramagnetic resonance oximetry and on the expression of proteins involved in tumour growth, metabolism and hypoxia...
July 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28756309/activation-of-brain-glucose-metabolism-ameliorating-cognitive-impairment-in-app-ps1-transgenic-mice-by-electroacupuncture
#15
Weilin Liu, Peiyuan Zhuo, Long Li, Hao Jin, Bingbing Lin, Yingzheng Zhang, Shengxiang Liang, Jie Wu, Jia Huang, Zhifu Wang, Ruhui Lin, Lidian Chen, Jing Tao
An essential feature of Alzheimer's disease (AD) is implicated in brain energy metabolic impairment that is considered underlying pathogenesis of cognitive impairment. Therefore, therapeutic interventions to allay cognitive deficits that target energy metabolism may be an efficacy strategy in AD. In this study, we found that electroacupuncture (EA) at the DU20 acupoint obviously increased glucose metabolism in specific brain regions such as cortex, hippocampus, cingulate gyrus, basal forebrain septum, brain stem, and cerebellum in APP/PS1 transgenic mice by animal (18)F-Fluoro-2-deoxy-D-Glucose ((18)F-FDG)/positron emission tomography (PET) imaging, accompanied by cognitive improvements in the spatial reference learning and memory and memory flexibility and novel object recognition performances...
July 26, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28756087/a-glimpse-of-membrane-transport-through-structures-advances-in-the-structural-biology-of-the-glut-glucose-transporters
#16
REVIEW
Nieng Yan
The cellular uptake of glucose is an essential physiological process, and movement of glucose across biological membranes requires specialized transporters. The major facilitator superfamily glucose transporters GLUTs, encoded by the SLC2A genes, have been a paradigm for functional, mechanistic, and structural understanding of solute transport in the past century. This review starts with a glimpse into the structural biology of membrane proteins and particularly membrane transport proteins, enumerating the landmark structures in the past 25years...
July 26, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28754302/immunometabolic-and-lipidomic-markers-associated-with-the-frailty-index-and-quality-of-life-in-aging-hiv-men-on-antiretroviral-therapy
#17
Hui-Ling Yeoh, Allen C Cheng, Catherine L Cherry, Jacquelyn M Weir, Peter J Meikle, Jennifer F Hoy, Suzanne M Crowe, Clovis S Palmer
Chronic immune activation persists despite antiretroviral therapy (ART) in HIV+ individuals and underpins an increased risk of age-related co-morbidities. We assessed the Frailty Index in older HIV+ Australian men on ART. Immunometabolic markers on monocytes and T cells were analyzed using flow cytometry, plasma innate immune activation markers by ELISA, and lipidomic profiling by mass spectrometry. The study population consisted of 80 HIV+ men with a median age of 59 (IQR, 56-65), and most had an undetectable viral load (92%)...
August 2017: EBioMedicine
https://www.readbyqxmd.com/read/28747976/immunohistochemical-localization-of-glucose-transporter-1-and-3-in-the-scrotal-and-abdominal-testes-of-a-dog
#18
Kyu Ri Hahn, Hyo Young Jung, Dae Young Yoo, Jong Whi Kim, Yang Hee Kim, Young Kwang Jo, Geon A Kim, Jin Young Chung, Jung Hoon Choi, In Koo Hwang, Goo Jang, Yeo Sung Yoon
Glucose is essential for testicular function; the uptake of carbohydrate-derived glucose by cells is mediated by glucose transporters (GLUTs). In the present study, we investigated the activity of GLUT1 and GLUT3, the two main isoforms of GLUTs found in testes, in the left scrotal and right abdominal testes of a German Shepherd dog. Immunohistochemical analysis showed that GLUT1 immunoreactivity was absent in the scrotal and abdominal testes. In contrast, weak to moderate GLUT3 immunoreactivity was observed in mature spermatocytes as well as spermatids in the scrotal testis...
June 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/28746873/deletion-of-glut1-and-glut3-reveals-multiple-roles-for-glucose-metabolism-in-platelet-and-megakaryocyte-function
#19
Trevor P Fidler, Robert A Campbell, Trevor Funari, Nicholas Dunne, Enrique Balderas Angeles, Elizabeth A Middleton, Dipayan Chaudhuri, Andrew S Weyrch, E Dale Abel
Anucleate platelets circulate in the blood to facilitate thrombosis and diverse immune functions. Platelet activation leading to clot formation correlates with increased glycogenolysis, glucose uptake, glucose oxidation, and lactic acid production. Simultaneous deletion of glucose transporter (GLUT) 1 and GLUT3 (double knockout [DKO]) specifically in platelets completely abolished glucose uptake. In DKO platelets, mitochondrial oxidative metabolism of non-glycolytic substrates, such as glutamate, increased...
July 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28739174/inhibition-of-mitochondrial-fission-prevents-hypoxia-induced-metabolic-shift-and-cellular-proliferation-of-pulmonary-arterial-smooth-muscle-cells
#20
Valentina Parra, Roberto Bravo-Sagua, Ignacio Norambuena-Soto, Carolina P Hernández-Fuentes, Andrés G Gómez-Contreras, Hugo E Verdejo, Rosemarie Mellado, Mario Chiong, Sergio Lavandero, Pablo F Castro
Inhibition of mitochondrial fission prevents hypoxia-induced metabolic shift and cellular proliferation of pulmonary arterial smooth muscle cells. Valentina Parra, Roberto Bravo-Sagua, Ignacio Norambuena-Soto, Carolina P. Hernández-Fuentes, Andrés G. Gómez-Contreras, Hugo E. Verdejo, Rosemarie Mellado, Mario Chiong, Sergio Lavandero, Pablo F. Castro. Chronic hypoxia exacerbates proliferation of pulmonary arterial smooth muscle cells (PASMC), thereby reducing the lumen of pulmonary arteries. This leads to poor blood oxygenation and cardiac work overload, which are the basis of diseases such as pulmonary artery hypertension (PAH)...
July 21, 2017: Biochimica et Biophysica Acta
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