keyword
MENU ▼
Read by QxMD icon Read
search

cxcl3

keyword
https://www.readbyqxmd.com/read/28989790/mechanistic-insights-into-molecular-evolution-of-species-specific-differential-glycosaminoglycan-binding-surfaces-in-growth-related-oncogene-chemokines
#1
Khushboo Gulati, Minal Jamsandekar, Krishna Mohan Poluri
Chemokines are chemotactic cytokines involved in leucocyte trafficking to infected tissue. Growth-related oncogene (GRO) chemokines namely CXCL1, CXCL2 and CXCL3 are neutrophil activating chemokines sharing a conserved three-dimensional structure, but encompassing functional diversity due to gene duplication and evolutionary events. However, the evolutionary mechanisms including selection pressures involved in diversification of GRO genes have not yet been characterized. Here, we performed comprehensive evolutionary analysis of GRO genes among different mammalian species...
September 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28977039/host-transcriptional-responses-following-ex-vivo-re-challenge-with-mycobacterium-tuberculosis-vary-with-disease-status
#2
Elaine A Yu, Serene H John, Elizabeth C Tablante, Christine A King, John Kenneth, David G Russell, Saurabh Mehta
The identification of immune correlates that are predictive of disease outcome for tuberculosis remains an ongoing challenge. To address this issue, we evaluated gene expression profiles from peripheral blood mononuclear cells following ex vivo challenge with Mycobacterium tuberculosis, among participants with active TB disease (ATBD, n = 10), latent TB infection (LTBI, n = 10), and previous active TB disease (after successful treatment; PTBD, n = 10), relative to controls (n = 10). Differential gene expression profiles were assessed by suppression-subtractive hybridization, dot blot, real-time polymerase chain reaction, and the comparative cycle threshold methods...
2017: PloS One
https://www.readbyqxmd.com/read/28933920/multi-tissue-transcriptomics-delineates-the-diversity-of-airway-t-cell-functions-in-asthma
#3
Akul Singhania, Joshua C Wallington, Caroline G Smith, Daniel Horowitz, Karl J Staples, Peter H Howarth, Stephan D Gadola, Ratko Djukanović, Christopher H Woelk, Timothy Sc Hinks
Asthma arises from the complex interplay of inflammatory pathways in diverse cell types and tissues. We sought to undertake a comprehensive transcriptomic assessment of the epithelium and airway T cells that remain understudied in asthma, and investigate interactions between multiple cells and tissues. Epithelial brushings and flow-sorted CD3+ T cells from sputum and bronchoalveolar lavage were obtained from healthy subjects (N=19) and asthmatic patients (mild, moderate and severe asthma; N=46). Gene expression was assessed using Affymetrix HT HG-U133+ PM GeneChips and results were validated by real-time quantitative PCR...
September 21, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28928065/molecular-cloning-and-biophysical-characterization-of-cxcl3-chemokine
#4
Khushboo Gulati, Krishnakant Gangele, Nipanshu Agarwal, Minal Jamsandekar, Dinesh Kumar, Krishna Mohan Poluri
CXCL3 is a neutrophil activating chemokine that belongs to GRO subfamily of CXC chemokines. GRO chemokine family comprises of three chemokines GRO α (CXCL1), GROβ (CXCL2), and GRO γ (CXCL3), which arose as a result of gene duplication events during the course of chemokine evolution. Although primary sequences of GRO chemokines are highly similar, they performs several protein specific functions in addition to their common property of neutrophil trafficking. However, the molecular basis for their differential functions has not well understood...
September 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28881574/prevention-of-colitis-associated-cancer-by-selective-targeting-of-immunoproteasome-subunit-lmp7
#5
Niyati Vachharajani, Thorsten Joeris, Maik Luu, Sabrina Hartmann, Sabine Pautz, Elena Jenike, Georgios Pantazis, Immo Prinz, Markus J Hofer, Ulrich Steinhoff, Alexander Visekruna
Chronic inflammation is a well-known risk factor in development of intestinal tumorigenesis, although the exact mechanisms underlying development of colitis-associated cancer (CAC) still remain obscure. The activity and function of immunoproteasome has been extensively analyzed in the context of inflammation and infectious diseases. Here, we show that the proteasomal immunosubunit LMP7 plays an essential role in development of CAC. Mice devoid of LMP7 were resistant to chronic inflammation and formation of neoplasia, and developed virtually no tumors after AOM/DSS treatment...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28874747/transcriptome-analysis-of-inflammation-related-gene-expression-in-endothelial-cells-activated-by-complement-masp-1
#6
Endre Schwaner, Zsuzsanna Németh, Péter K Jani, Erika Kajdácsi, Márta L Debreczeni, Zoltán Doleschall, József Dobó, Péter Gál, János Rigó, Kinga András, Tamás Hegedűs, László Cervenak
Mannan-binding lectin-associated serine protease 1 (MASP-1), the most abundant enzyme of the complement lectin pathway, is able to stimulate human umbilical vein endothelial cells (HUVECs) to alter the expression of several cytokines and adhesion molecules. This study has assessed to what extent MASP-1 is able to modify the transcriptional pattern of inflammation-related (IR) genes in HUVECs. We utilized Agilent microarray to analyse the effects of recombinant MASP-1 (rMASP-1) in HUVECs, on a set of 884 IR genes...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28842479/tnf-%C3%AE-promotes-nuclear-enrichment-of-tonebp-nfat5-to-selectively-control-inflammatory-but-not-osmoregulatory-responses-in-nucleus-pulposus-cells
#7
Zariel I Johnson, Alexandra C Doolittle, Joseph W Snuggs, Irving M Shapiro, Christine L Le Maitre, Makarand V Risbud
Intervertebral disc degeneration (IDD) causes chronic back pain and is linked to production of proinflammatory molecules by nucleus pulposus (NP) and other disc cells. Activation of TonicityResponsive Enhancer-Binding Protein (TonEBP)/NFAT5 by non-osmotic stimuli, including pro-inflammatory molecules, occurs in cells involved in immune response. However, whether inflammatory stimuli activate TonEBP in NP cells and if TonEBP controls inflammation during IDD is unknown. We show that TNF-α, but not IL-1β or LPS, promoted nuclear enrichment of TonEBP protein...
August 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28736425/cd133-regulates-il-1%C3%AE-signaling-and-neutrophil-recruitment-in-glioblastoma
#8
Seon Yong Lee, Jun-Kyum Kim, Hee-Young Jeon, Seok Won Ham, Hyunggee Kim
CD133, a pentaspan transmembrane glycoprotein, is generally used as a cancer stem cell marker in various human malignancies, but its biological function in cancer cells, especially in glioma cells, is largely unknown. Here, we demonstrated that forced expression of CD133 increases the expression of IL-1β and its downstream chemokines, namely, CCL3, CXCL3 and CXCL5, in U87MG glioma cells. Although there were no apparent changes in cell growth and sphere formation in vitro and tumor growth in vivo, in vitro trans-well studies and in vivo tumor xenograft assays showed that neutrophil recruitment was markedly increased by the ectopic expression of CD133...
July 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28708839/tumor-necrosis-factor-dynamically-regulates-the-mrna-stabilome-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#9
Konstantinos Loupasakis, David Kuo, Upneet K Sokhi, Christopher Sohn, Bethany Syracuse, Eugenia G Giannopoulou, Sung Ho Park, Hyelim Kang, Gunnar Rätsch, Lionel B Ivashkiv, George D Kalliolias
During rheumatoid arthritis (RA), Tumor Necrosis Factor (TNF) activates fibroblast-like synoviocytes (FLS) inducing in a temporal order a constellation of genes, which perpetuate synovial inflammation. Although the molecular mechanisms regulating TNF-induced transcription are well characterized, little is known about the impact of mRNA stability on gene expression and the impact of TNF on decay rates of mRNA transcripts in FLS. To address these issues we performed RNA sequencing and genome-wide analysis of the mRNA stabilome in RA FLS...
2017: PloS One
https://www.readbyqxmd.com/read/28680854/bovine-endometrial-epithelial-cells-scale-their-pro-inflammatory-response-in-vitro-to-pathogenic-trueperella-pyogenes-isolated-from-the-bovine-uterus-in-a-strain-specific-manner
#10
Mohammad Ibrahim, Sarah Peter, Karen Wagener, Marc Drillich, Monika Ehling-Schulz, Ralf Einspanier, Christoph Gabler
Among different bacteria colonizing the bovine uterus, Trueperella pyogenes is found to be associated with clinical endometritis (CE). The ability of cows to defend against T. pyogenes infections depends on the virulence of invading bacteria and on the host's innate immunity. Therefore, to gain insights into bacterial factors contributing to the interplay of this host pathogen, two strains of T. pyogenes were included in this study: one strain (TP2) was isolated from the uterus of a postpartum dairy cow developing CE and a second strain (TP5) was isolated from a uterus of a healthy cow...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28626076/cardiac-fibroblast-transcriptome-analyses-support-a-role-for-interferogenic-profibrotic-and-inflammatory-genes-in-anti-ssa-ro-associated-congenital-heart-block
#11
Robert M Clancy, Androo J Markham, Tanisha Jackson, Sara E Rasmussen, Miroslav Blumenberg, Jill P Buyon
The signature lesion of SSA/Ro autoantibody-associated congenital heart block (CHB) is fibrosis and a macrophage infiltrate, supporting an experimental focus on cues influencing the fibroblast component. The transcriptomes of human fetal cardiac fibroblasts were analyzed using two complementary approaches. Cardiac injury conditions were simulated in vitro by incubating human fetal cardiac fibroblasts with supernatants from macrophages transfected with the SSA/Ro-associated noncoding Y ssRNA. The top 10 upregulated transcripts in the stimulated fibroblasts reflected a type I interferon (IFN) response [e...
September 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28546923/homocysteine-mediates-transcriptional-changes-of-the-inflammatory-pathway-signature-genes-in-human-retinal-pigment-epithelial-cells
#12
Mahavir Singh, Suresh C Tyagi
AIM: To test whether homocysteine (Hcy) can influence the transcriptional profile, we hypothesized that Hcy can lead to the induction of proinflammatory molecules in the retinal cells of aging people. METHODS: An unbiased in vitro inflammatory pathway focused study was designed employing retinal pigment epithelial (RPE) cell line, ARPE-19. Cells were cultured in the presence or absence of Hcy to capture target genes' expression profile. Three different concentrations of Hcy were added in the culture medium of confluent monolayers...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28533735/esm-1-sirna-knockdown-decreased-migration-and-expression-of-cxcl3-in-prostate-cancer-cells
#13
Juan Rebollo, Jan Geliebter, Niradiz Reyes
Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells...
March 2017: International Journal of Biomedical Science: IJBS
https://www.readbyqxmd.com/read/28387727/cellular-response-to-titanium-dioxide-nanoparticles-in-intestinal-epithelial-caco-2-cells-is-dependent-on-endocytosis-associated-structures-and-mediated-by-egfr
#14
Kristin Krüger, Katrin Schrader, Martin Klempt
Titanium dioxide (TiO₂) is one of the most applied nanomaterials and widely used in food and non-food industries as an additive or coating material (E171). It has been shown that E171 contains up to 37% particles which are smaller than 100 nm and that TiO₂ nanoparticles (NPs) induce cytotoxicity and inflammation. Using a nuclear factor Kappa-light-chain enhancer of activated B cells (NF-κB) reporter cell line (Caco-2(nfkb-RE)), Real time polymerase chain reaction (PCR), and inhibition of dynamin and clathrin, it was shown that cellular responses induced by 5 nm and 10 nm TiO₂ NPs (nominal size) depends on endocytic processes...
April 7, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28178176/nlrp12-inflammasome-expression-in-the-rat-brain-in-response-to-lps-during-morphine-tolerance
#15
Sulie L Chang, Wenfei Huang, Xin Mao, Sabroni Sarkar
Morphine, an effective but addictive analgesic, can profoundly affect the inflammatory response to pathogens, and long-term use can result in morphine tolerance. Inflammasomes are protein complexes involved in the inflammatory response. The nucleotide-binding oligomerization domain-like receptor (NLR) Family Pyrin Domain Containing (NLRP) 12 (NLRP12) inflammasome has been reported to have anti-inflammatory activity. In this study, we examined the expression of NLRP12 inflammasome related genes in the adult F344 rat brain in response to the bacterial endotoxin lipopolysaccharide (LPS) in the presence and absence of morphine tolerance...
February 6, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28166974/endometrial-mrna-expression-of-selected-pro-inflammatory-factors-and-mucins-in-repeat-breeder-cows-with-and-without-subclinical-endometritis
#16
K Wagener, H Pothmann, I Prunner, S Peter, R Erber, C Aurich, M Drillich, C Gabler
Repeat breeder cows (RBC) are defined as cyclic cows without clinical abnormalities that fail to conceive after at least three subsequent inseminations. Previous studies have elucidated cellular defence mechanisms in the bovine uterus but detailed information on inflammatory events of endometrial cells in RBC is still lacking. Thus, the objective of this study was to analyse endometrial mRNA expression of selected transcripts associated with uterine inflammatory processes. Cytobrush samples from 91 RBC and 11 synchronised heifers with no history of gynaecological abnormalities (controls, CON) were collected...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28161477/sequential-transmigration-of-polymorphonuclear-cells-and-naive-cd3-t-lymphocytes-across-the-blood-cerebrospinal-fluid-barrier-in-vitro-following-infection-with-echovirus-30
#17
Tobias Dahm, Franziska Frank, Ortwin Adams, Holger A Lindner, Hiroshi Ishikawa, Christel Weiss, Christian Schwerk, Horst Schroten, Tobias Tenenbaum, Henriette Rudolph
Viral meningitis by non-polio enteroviruses (NPEV) is a major public health burden causing fatal outcomes especially in the younger population. Strong evidence exists that the blood-cerebrospinal-fluid (CSF) barrier (BCSFB) serves as an entry point for enterovirus and leucocytes into the central nervous system (CNS). Moreover, analysis of clinical CSF specimens of patients with a NPEV infection revealed a predominance of polymorphonuclear granulocytes (PMN) in the early phase and mononuclear cells in the later course of meningitis...
February 1, 2017: Virus Research
https://www.readbyqxmd.com/read/28120235/identifying-key-genes-associated-with-hirschsprung-s-disease-based-on-bioinformatics-analysis-of-rna-sequencing-data
#18
Wei-Kang Pan, Ya-Fei Zhang, Hui Yu, Ya Gao, Bai-Jun Zheng, Peng Li, Chong Xie, Xin Ge
BACKGROUND: Hirschsprung's disease (HSCR) is a type of megacolon induced by deficiency or dysfunction of ganglion cells in the distal intestine and is associated with developmental disorders of the enteric nervous system. To explore the mechanisms of HSCR, we analyzed the RNA-sequencing data of the expansion and the narrow segments of colon tissues separated from children with HSCR. METHODS: RNA-sequencing of the expansion segments and the narrow segments of colon tissues isolated from children with HSCR was performed...
June 2017: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/28018222/suppression-of-medulloblastoma-lesions-by-forced-migration-of-preneoplastic-precursor-cells-with-intracerebellar-administration-of-the-chemokine-cxcl3
#19
Manuela Ceccarelli, Laura Micheli, Felice Tirone
Medulloblastoma (MB), tumor of the cerebellum, remains a leading cause of cancer-related mortality in childhood. We previously showed, in a mouse model of spontaneous MB (Ptch1(+/-)/Tis21(-/-)), that a defect of the migration of cerebellar granule neuron precursor cells (GCPs) correlates with an increased frequency of MB. This occurs because GCPs, rather than migrating internally and differentiating, remain longer in the proliferative area at the cerebellar surface, becoming targets of transforming insults...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27965576/functional-genomics-identifies-tis21-dependent-mechanisms-and-putative-cancer-drug-targets-underlying-medulloblastoma-shh-type-development
#20
Giulia Gentile, Manuela Ceccarelli, Laura Micheli, Felice Tirone, Sebastiano Cavallaro
We have recently generated a novel medulloblastoma (MB) mouse model with activation of the Shh pathway and lacking the MB suppressor Tis21 (Patched1(+/-)/Tis21(KO) ). Its main phenotype is a defect of migration of the cerebellar granule precursor cells (GCPs). By genomic analysis of GCPs in vivo, we identified as drug target and major responsible of this defect the down-regulation of the promigratory chemokine Cxcl3. Consequently, the GCPs remain longer in the cerebellum proliferative area, and the MB frequency is enhanced...
2016: Frontiers in Pharmacology
keyword
keyword
116269
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"