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https://www.readbyqxmd.com/read/28736425/cd133-regulates-il-1%C3%AE-signaling-and-neutrophil-recruitment-in-glioblastoma
#1
Seon Yong Lee, Jun-Kyum Kim, Hee-Young Jeon, Seok Won Ham, Hyunggee Kim
CD133, a pentaspan transmembrane glycoprotein, is generally used as a cancer stem cell marker in various human malignancies, but its biological function in cancer cells, especially in glioma cells, is largely unknown. Here, we demonstrated that forced expression of CD133 increases the expression of IL-1β and its downstream chemokines, namely, CCL3, CXCL3 and CXCL5, in U87MG glioma cells. Although there were no apparent changes in cell growth and sphere formation in vitro and tumor growth in vivo, in vitro trans-well studies and in vivo tumor xenograft assays showed that neutrophil recruitment was markedly increased by the ectopic expression of CD133...
July 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28708839/tumor-necrosis-factor-dynamically-regulates-the-mrna-stabilome-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#2
Konstantinos Loupasakis, David Kuo, Upneet K Sokhi, Christopher Sohn, Bethany Syracuse, Eugenia G Giannopoulou, Sung Ho Park, Hyelim Kang, Gunnar Rätsch, Lionel B Ivashkiv, George D Kalliolias
During rheumatoid arthritis (RA), Tumor Necrosis Factor (TNF) activates fibroblast-like synoviocytes (FLS) inducing in a temporal order a constellation of genes, which perpetuate synovial inflammation. Although the molecular mechanisms regulating TNF-induced transcription are well characterized, little is known about the impact of mRNA stability on gene expression and the impact of TNF on decay rates of mRNA transcripts in FLS. To address these issues we performed RNA sequencing and genome-wide analysis of the mRNA stabilome in RA FLS...
2017: PloS One
https://www.readbyqxmd.com/read/28680854/bovine-endometrial-epithelial-cells-scale-their-pro-inflammatory-response-in-vitro-to-pathogenic-trueperella-pyogenes-isolated-from-the-bovine-uterus-in-a-strain-specific-manner
#3
Mohammad Ibrahim, Sarah Peter, Karen Wagener, Marc Drillich, Monika Ehling-Schulz, Ralf Einspanier, Christoph Gabler
Among different bacteria colonizing the bovine uterus, Trueperella pyogenes is found to be associated with clinical endometritis (CE). The ability of cows to defend against T. pyogenes infections depends on the virulence of invading bacteria and on the host's innate immunity. Therefore, to gain insights into bacterial factors contributing to the interplay of this host pathogen, two strains of T. pyogenes were included in this study: one strain (TP2) was isolated from the uterus of a postpartum dairy cow developing CE and a second strain (TP5) was isolated from a uterus of a healthy cow...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28626076/cardiac-fibroblast-transcriptome-analyses-support-a-role-for-interferogenic-profibrotic-and-inflammatory-genes-in-anti-ssa-ro-associated-congenital-heart-block
#4
Robert M Clancy, Androo J Markham, Tanisha Jackson, Sara E Rasmussen, Miroslav Blumenberg, Jill P Buyon
The signature lesion of SSA/Ro autoantibody-associated congenital heart block (CHB) is fibrosis and a macrophage infiltrate, supporting an experimental focus on cues influencing the fibroblast component. The transcriptomes of human fetal cardiac fibroblasts were analyzed using two complementary approaches. Cardiac injury conditions were simulated in vitro by incubating human fetal cardiac fibroblasts with supernatants from macrophages transfected with the SSA/Ro-associated hY3. The top ten upregulated transcripts in the stimulated fibroblasts reflected a type I interferon (IFN) response (e...
June 16, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28546923/homocysteine-mediates-transcriptional-changes-of-the-inflammatory-pathway-signature-genes-in-human-retinal-pigment-epithelial-cells
#5
Mahavir Singh, Suresh C Tyagi
AIM: To test whether homocysteine (Hcy) can influence the transcriptional profile, we hypothesized that Hcy can lead to the induction of proinflammatory molecules in the retinal cells of aging people. METHODS: An unbiased in vitro inflammatory pathway focused study was designed employing retinal pigment epithelial (RPE) cell line, ARPE-19. Cells were cultured in the presence or absence of Hcy to capture target genes' expression profile. Three different concentrations of Hcy were added in the culture medium of confluent monolayers...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28533735/esm-1-sirna-knockdown-decreased-migration-and-expression-of-cxcl3-in-prostate-cancer-cells
#6
Juan Rebollo, Jan Geliebter, Niradiz Reyes
Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells...
March 2017: International Journal of Biomedical Science: IJBS
https://www.readbyqxmd.com/read/28387727/cellular-response-to-titanium-dioxide-nanoparticles-in-intestinal-epithelial-caco-2-cells-is-dependent-on-endocytosis-associated-structures-and-mediated-by-egfr
#7
Kristin Krüger, Katrin Schrader, Martin Klempt
Titanium dioxide (TiO₂) is one of the most applied nanomaterials and widely used in food and non-food industries as an additive or coating material (E171). It has been shown that E171 contains up to 37% particles which are smaller than 100 nm and that TiO₂ nanoparticles (NPs) induce cytotoxicity and inflammation. Using a nuclear factor Kappa-light-chain enhancer of activated B cells (NF-κB) reporter cell line (Caco-2(nfkb-RE)), Real time polymerase chain reaction (PCR), and inhibition of dynamin and clathrin, it was shown that cellular responses induced by 5 nm and 10 nm TiO₂ NPs (nominal size) depends on endocytic processes...
April 7, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28178176/nlrp12-inflammasome-expression-in-the-rat-brain-in-response-to-lps-during-morphine-tolerance
#8
Sulie L Chang, Wenfei Huang, Xin Mao, Sabroni Sarkar
Morphine, an effective but addictive analgesic, can profoundly affect the inflammatory response to pathogens, and long-term use can result in morphine tolerance. Inflammasomes are protein complexes involved in the inflammatory response. The nucleotide-binding oligomerization domain-like receptor (NLR) Family Pyrin Domain Containing (NLRP) 12 (NLRP12) inflammasome has been reported to have anti-inflammatory activity. In this study, we examined the expression of NLRP12 inflammasome related genes in the adult F344 rat brain in response to the bacterial endotoxin lipopolysaccharide (LPS) in the presence and absence of morphine tolerance...
February 6, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28166974/endometrial-mrna-expression-of-selected-pro-inflammatory-factors-and-mucins-in-repeat-breeder-cows-with-and-without-subclinical-endometritis
#9
K Wagener, H Pothmann, I Prunner, S Peter, R Erber, C Aurich, M Drillich, C Gabler
Repeat breeder cows (RBC) are defined as cyclic cows without clinical abnormalities that fail to conceive after at least three subsequent inseminations. Previous studies have elucidated cellular defence mechanisms in the bovine uterus but detailed information on inflammatory events of endometrial cells in RBC is still lacking. Thus, the objective of this study was to analyse endometrial mRNA expression of selected transcripts associated with uterine inflammatory processes. Cytobrush samples from 91 RBC and 11 synchronised heifers with no history of gynaecological abnormalities (controls, CON) were collected...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28161477/sequential-transmigration-of-polymorphonuclear-cells-and-naive-cd3-t-lymphocytes-across-the-blood-cerebrospinal-fluid-barrier-in-vitro-following-infection-with-echovirus-30
#10
Tobias Dahm, Franziska Frank, Ortwin Adams, Holger A Lindner, Hiroshi Ishikawa, Christel Weiss, Christian Schwerk, Horst Schroten, Tobias Tenenbaum, Henriette Rudolph
Viral meningitis by non-polio enteroviruses (NPEV) is a major public health burden causing fatal outcomes especially in the younger population. Strong evidence exists that the blood-cerebrospinal-fluid (CSF) barrier (BCSFB) serves as an entry point for enterovirus and leucocytes into the central nervous system (CNS). Moreover, analysis of clinical CSF specimens of patients with a NPEV infection revealed a predominance of polymorphonuclear granulocytes (PMN) in the early phase and mononuclear cells in the later course of meningitis...
February 1, 2017: Virus Research
https://www.readbyqxmd.com/read/28120235/identifying-key-genes-associated-with-hirschsprung-s-disease-based-on-bioinformatics-analysis-of-rna-sequencing-data
#11
Wei-Kang Pan, Ya-Fei Zhang, Hui Yu, Ya Gao, Bai-Jun Zheng, Peng Li, Chong Xie, Xin Ge
BACKGROUND: Hirschsprung's disease (HSCR) is a type of megacolon induced by deficiency or dysfunction of ganglion cells in the distal intestine and is associated with developmental disorders of the enteric nervous system. To explore the mechanisms of HSCR, we analyzed the RNA-sequencing data of the expansion and the narrow segments of colon tissues separated from children with HSCR. METHODS: RNA-sequencing of the expansion segments and the narrow segments of colon tissues isolated from children with HSCR was performed...
June 2017: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/28018222/suppression-of-medulloblastoma-lesions-by-forced-migration-of-preneoplastic-precursor-cells-with-intracerebellar-administration-of-the-chemokine-cxcl3
#12
Manuela Ceccarelli, Laura Micheli, Felice Tirone
Medulloblastoma (MB), tumor of the cerebellum, remains a leading cause of cancer-related mortality in childhood. We previously showed, in a mouse model of spontaneous MB (Ptch1(+/-)/Tis21(-/-)), that a defect of the migration of cerebellar granule neuron precursor cells (GCPs) correlates with an increased frequency of MB. This occurs because GCPs, rather than migrating internally and differentiating, remain longer in the proliferative area at the cerebellar surface, becoming targets of transforming insults...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27965576/functional-genomics-identifies-tis21-dependent-mechanisms-and-putative-cancer-drug-targets-underlying-medulloblastoma-shh-type-development
#13
Giulia Gentile, Manuela Ceccarelli, Laura Micheli, Felice Tirone, Sebastiano Cavallaro
We have recently generated a novel medulloblastoma (MB) mouse model with activation of the Shh pathway and lacking the MB suppressor Tis21 (Patched1(+/-)/Tis21(KO) ). Its main phenotype is a defect of migration of the cerebellar granule precursor cells (GCPs). By genomic analysis of GCPs in vivo, we identified as drug target and major responsible of this defect the down-regulation of the promigratory chemokine Cxcl3. Consequently, the GCPs remain longer in the cerebellum proliferative area, and the MB frequency is enhanced...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27916291/intratumoral-immunization-by-p19arf-and-interferon-%C3%AE-gene-transfer-in-a-heterotopic-mouse-model-of-lung-carcinoma
#14
João Paulo Portela Catani, Ruan F V Medrano, Aline Hunger, Paulo Del Valle, Sandy Adjemian, Daniela Bertolini Zanatta, Guido Kroemer, Eugenia Costanzi-Strauss, Bryan E Strauss
Therapeutic strategies that act by eliciting and enhancing antitumor immunity have been clinically validated as an effective treatment modality but may benefit from the induction of both cell death and immune activation as primary stimuli. Using our AdRGD-PG adenovector platform, we show here for the first time that in situ gene transfer of p19Arf and interferon-β (IFNβ) in the LLC1 mouse model of lung carcinoma acts as an immunotherapy. Although p19Arf is sufficient to induce cell death, only its pairing with IFNβ significantly induced markers of immunogenic cell death...
December 2016: Translational Oncology
https://www.readbyqxmd.com/read/27832807/sex-specific-variation-in-signaling-pathways-and-gene-expression-patterns-in-human-leukocytes-in-response-to-endotoxin-and-exercise
#15
Asghar Abbasi, Rodolfo de Paula Vieira, Felix Bischof, Michael Walter, Masoud Movassaghi, Nicole C Berchtold, Andreas M Niess, Carl W Cotman, Hinnak Northoff
BACKGROUND: While exercise effects on the immune system have received increasing attention in recent years, it remains unclear to what extent gender and fluctuations in sex hormones during menstrual cycle influence immunological responses to exercise. METHODS: We investigated mRNA changes induced through exhaustive exercise (half-marathon; pre-exercise and post-exercise [30 min, 3 h, 24 h] on whole blood cultures ± lipopolysaccharide [LPS] [1 h]) with a specific focus on sex differences (men vs women in luteal phase) as an extension of our previous study...
November 10, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27829437/in-vivo-inhibition-of-mir-155-significantly-alters-post-stroke-inflammatory-response
#16
Juan Carlos Pena-Philippides, Ernesto Caballero-Garrido, Tamar Lordkipanidze, Tamara Roitbak
BACKGROUND: MicroRNA miR-155 is implicated in modulation of the inflammatory processes in various pathological conditions. In our previous studies, we demonstrated that in vivo inhibition of miR-155 promotes functional recovery after mouse experimental stroke. In the present study, we explored if this beneficial effect is associated with miR-155 inhibition-induced alterations in post-stroke inflammatory response. METHODS: Intravenous injections of a specific miR-155 inhibitor were initiated at 48 h after mouse distal middle cerebral artery occlusion (dMCAO)...
November 9, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27698747/co-expressed-differentially-expressed-genes-and-long-non-coding-rnas-involved-in-the-celecoxib-treatment-of-gastric-cancer-an-rna-sequencing-analysis
#17
Bin Song, Juan Du, Ye Feng, Yong-Jian Gao, Ji-Sheng Zhao
The aim of the present study was to investigate the mechanisms of long non-coding RNAs (lncRNAs) in a gastric cancer cell line treated with celecoxib. The human gastric carcinoma cell line NCI-N87 was treated with 15 µM celecoxib for 72 h (celecoxib group) and an equal volume of dimethylsulfoxide (control group), respectively. Libraries were constructed by NEBNext Ultra RNA Library Prep kit for Illumina. Paired-end RNA sequencing reads were aligned to a human hg19 reference genome using TopHat2. Differentially expressed genes (DEGs) and lncRNAs were identified using Cuffdiff...
October 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27682863/chemokine-cxcl1-may-serve-as-a-potential-molecular-target-for-hepatocellular-carcinoma
#18
Ke-Qi Han, Hui Han, Xue-Qun He, Lei Wang, Xiao-Dong Guo, Xue-Ming Zhang, Jie Chen, Quan-Gang Zhu, Hua Nian, Xiao-Feng Zhai, Ma-Wei Jiang
The purpose of this study was to screen for changes in chemokine and chemokine-related genes that are expressed in hepatocellular carcinoma (HCC) as potential markers of HCC progression. Total RNA was extracted from tumor and peritumor tissues from mice with HCC and analyzed using a PCR microarray comprising 98 genes. Changes in gene expression of threefold or more were screened and subsequently confirmed by immunohistochemical analyses and western blotting. Furthermore, whether chemokine knockdown by RNA interference (RNAi) could significantly suppress tumor growth in vivo was also evaluated...
September 28, 2016: Cancer Medicine
https://www.readbyqxmd.com/read/27512010/cxcl3-positively-regulates-adipogenic-differentiation
#19
Joji Kusuyama, Anna Komorizono, Kenjiro Bandow, Tomokazu Ohnishi, Tetsuya Matsuguchi
Chemokines are a family of cytokines inducing cell migration and inflammation. Recent reports have implicated the roles of chemokines in cell differentiation. However, little is known about the functional roles of chemokines in adipocytes. Here, we explored gene expression levels of chemokines and chemokine receptors during adipogenic differentiation. We have found that two chemokines, chemokine (C-X-C motif) ligand 3 (CXCL3) and CXCL13, as well as CXC chemokine receptor 2 (CXCR2), a CXCL3 receptor, are highly expressed in mature adipocytes...
October 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/27493972/actions-of-thyroid-hormone-analogues-on-chemokines
#20
REVIEW
Paul J Davis, Gennadi V Glinsky, Hung-Yun Lin, Shaker A Mousa
The extracellular domain of plasma membrane integrin αvβ3 contains a receptor for thyroid hormone (L-thyroxine, T4; 3,5,3'-triiodo-L-thyronine, T3); this receptor also binds tetraiodothyroacetic acid (tetrac), a derivative of T4. Tetrac inhibits the binding of T4 and T3 to the integrin. Fractalkine (CX3CL1) is a chemokine relevant to inflammatory processes in the CNS that are microglia-dependent but also important to normal brain development. Expression of the CX3CL1 gene is downregulated by tetrac, suggesting that T4 and T3 may stimulate fractalkine expression...
2016: Journal of Immunology Research
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