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Mariam Meddeb, Wassila Carpentier, Nicolas Cagnard, Sophie Nadaud, Antoine Grillon, Cathy Barthel, Sylvie Josiane De Martino, Benoît Jaulhac, Nathalie Boulanger, Frédéric Schramm
In Lyme borreliosis, the skin is the key site for bacterial inoculation by the infected tick and for cutaneous manifestations. We previously showed that different strains of Borrelia burgdorferi sensu stricto isolated from tick and from different clinical stages of the Lyme borreliosis (erythema migrans, and acrodermatitis chronica atrophicans) elicited a very similar transcriptional response in normal human dermal fibroblasts. In this study, using whole transcriptome microarray chips, we aimed to compare the transcriptional response of normal human dermal fibroblasts stimulated by 3 Borrelia burgdorferi sensu lato strains belonging to 3 main pathogenic species (B...
2016: PloS One
X Huang, T Huang, W Deng, G Yan, H Qiu, Y Huang, S Ke, Y Hou, Y Zhang, Z Zhang, S Fang, L Zhou, B Yang, J Ren, H Ai, L Huang
Prevalence of swine respiratory disease causes poor growth performance in and serious economic losses to the swine industry. In this study, a categorical trait of enzootic pneumonia-like (EPL) score representing the infection gradient of a respiratory disease, more likely enzootic pneumonia, was recorded in a herd of 332 Chinese Erhualian pigs. According to their EPL scores and the disease effect on weight gains, these pigs were grouped into controls (EPL score ≤ 1) and cases (EPL score > 1). The weight gain of the case group reduced significantly at days 180, 210, 240 and 300 as compared to the control group...
September 12, 2016: Animal Genetics
Mona Elsafadi, Muthurangan Manikandan, Muhammad Atteya, Jamil Amjad Hashmi, Zafar Iqbal, Abdullah Aldahmash, Musaad Alfayez, Moustapha Kassem, Amer Mahmood
Human bone marrow-derived stromal stem cells (hBMSC) exhibit multiple functions, including differentiation into skeletal cells (progenitor function), hematopoiesis support, and immune regulation (nonprogenitor function). We have previously demonstrated the presence of morphological and functional heterogeneity of hBMSC cultures. In the present study, we characterized in detail two hTERT-BMSC clonal cell populations termed here CL1 and CL2 that represent an opposing phenotype with respect to morphology, markers expression: alkaline phosphatase (ALP) and CD146, and ex vivo differentiation potential...
2016: Stem Cells International
Robert H Brophy, Linda J Sandell, Muhammad Farooq Rai
BACKGROUND: Meniscus tears are classified as traumatic or degenerative based on the tear pattern. There is little evidence demonstrating biological differences between the 2 tear types. HYPOTHESIS: Gene expression signatures in the injured meniscus are different between traumatic (vertical) and degenerative (complex, horizontal, or flap) tears. STUDY DESIGN: Controlled laboratory study. METHODS: Samples of the torn meniscus from the white-white zone were removed at the time of clinically indicated partial meniscectomy from 48 patients (37 with degenerative tears and 11 with traumatic tears)...
September 7, 2016: American Journal of Sports Medicine
Hamidreza Moazzeni, Mohammad Taghi Akbari, Shahin Yazdani, Elahe Elahi
The transcription factor PITX2 is implicated in glaucoma pathology. In an earlier study we had used microarray analysis to identify genes in the trabecular meshwork (TM) that are affected by knock down of PITX2. Here, those studies were pursued to identify genes that are direct targets of PITX2 and that may be relevant to glaucoma. Initially, bioinformatics tools were used to select among the genes that had been affected by PITX2 knock down those that have PITX2 binding sites and that may be involved in glaucoma related functions...
November 15, 2016: Gene
Ruiyang Zhang, Weiyun Zhu, Shengyong Mao
BACKGROUND: The objective of this study was to characterize the mRNA expression profile related to rumen epithelial inflammation through the in vivo and in vitro experiments. In the in vivo experiment, rumen papillae were collected from four dairy cows adapted to either a 40 % (LC) or 70 % (HC) concentrate feeds for microarray analysis. RESULTS: Results showed that 245 differentially expressed genes (DEGs) were detected in the cows fed the HC relative to the LC diet...
2016: Journal of Animal Science and Biotechnology
Rosa Mistica C Ignacio, Carla R Gibbs, Eun-Sook Lee, Deok-Soo Son
Obesity is characterized as an accumulation of adipose tissue mass represented by chronic, low-grade inflammation. Obesity-derived inflammation involves chemokines as important regulators contributing to the pathophysiology of obesity-related diseases such as cardiovascular disease, diabetes and some cancers. The obesity-driven chemokine network is poorly understood. Here, we identified the profiles of chemokine signature between human preadipocytes and adipocytes, using PCR arrays and qRT-PCR. Both preadipocytes and adipocytes showed absent or low levels in chemokine receptors in spite of some changes...
June 2016: Immune Network
Amy Sinclair, Laura Park, Mansi Shah, Mark Drotar, Simon Calaminus, Lisa E M Hopcroft, Ross Kinstrie, Amelie V Guitart, Karen Dunn, Sheela A Abraham, Owen Sansom, Alison M Michie, Laura Machesky, Kamil R Kranc, Gerard J Graham, Francesca Pellicano, Tessa L Holyoake
The regulation of hematopoietic stem cell (HSC) survival and self-renewal within the bone marrow (BM) niche is not well understood. We therefore investigated global transcriptomic profiling of normal human HSC/hematopoietic progenitor cells [HPCs], revealing that several chemokine ligands (CXCL1-4, CXCL6, CXCL10, CXCL11, and CXCL13) were upregulated in human quiescent CD34(+)Hoescht(-)Pyronin Y(-) and primitive CD34(+)38(-), as compared with proliferating CD34(+)Hoechst(+)Pyronin Y(+) and CD34(+)38(+) stem/progenitor cells...
July 21, 2016: Blood
Yoshio Kiku, Yuya Nagasawa, Fuyuko Tanabe, Kazue Sugawara, Atsushi Watababe, Eiji Hata, Tomomi Ozawa, Kei-Ichi Nakajima, Toshiro Arai, Tomohito Hayashi
Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations...
May 20, 2016: Journal of Veterinary Medical Science
Steve Oghumu, Thomas J Knobloch, Cesar Terrazas, Sanjay Varikuti, Jennifer Ahn-Jarvis, Claire E Bollinger, Hans Iwenofu, Christopher M Weghorst, Abhay R Satoskar
Oral cancer kills about 1 person every hour each day in the United States and is the sixth most prevalent cancer worldwide. The pro-inflammatory cytokine 'macrophage migration inhibitory factor' (MIF) has been shown to be expressed in oral cancer patients, yet its precise role in oral carcinogenesis is not clear. In this study, we examined the impact of global Mif deletion on the cellular and molecular process occurring during oral carcinogenesis using a well-established mouse model of oral cancer with the carcinogen 4-nitroquinoline-1-oxide (4NQO)...
September 15, 2016: International Journal of Cancer. Journal International du Cancer
Ping Chen, Zhengzi Yi, Weijia Zhang, Mary E Klotman, Benjamin K Chen
OBJECTIVE: Viral replication and interstitial inflammation play important roles in the pathogenesis of HIV-associated nephropathy. Cell-cell interactions between renal tubule epithelial cells (RTECs) and HIV-infected T cells can trigger efficient virus internalization and viral gene expression by RTEC. To understand how HIV replication initiates HIV-associated nephropathy, we studied the cellular response of RTECs to HIV, examining the transcriptional profiles of primary RTECs exposed to cell-free HIV or HIV-infected T cells...
July 31, 2016: AIDS
Zhenghong Li, Qidi Zhang, Qingqing Zhang, Mingyi Xu, Ying Qu, Xiaobo Cai, Lungen Lu
Hepatocyte proliferation and collagen I (COLI) secretion are important processes during liver regeneration. This study aimed to investigate the role of CXCL6 in hepatocyte proliferation and COLI secretion. Serum CXCL6 levels in patients with chronic hepatitis B (CHB) were examined and the effects of CXCL6 on the proliferation of L02 hepatocytes and the secretion of COLI from LX2 human hepatic stellate cells were evaluated. We found that serum CXCL6 levels increased gradually with disease progression of CHB, and there was positive correlation between serum CXCL6 level and alanine transaminase (ALT) and aspartate transaminase (AST)...
June 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Wenwen Yan, Siwan Wen, Lemin Wang, Qianglin Duan, Lin Ding
OBJECTIVE: To investigate the differential gene expression of cytokines and compare their impacts on the immune functions among the acute myocardial infarction patients (AMI), the stable angina patients (SA) and the controls. METHODS: 20 patients with AMI, 20 patients with SA and 20 healthy volunteers were recruited into the study. Whole human genome microarray analysis was used to detect the gene expression differences in interferons, interleukins, chemokines, tumor necrosis factors and associated receptors in peripheral blood mononuclear cells (PBMCs) among three groups...
2015: International Journal of Clinical and Experimental Medicine
Anneleen Mortier, Mieke Gouwy, Jo Van Damme, Paul Proost, Sofie Struyf
Post-translational modification of chemokines is an essential regulatory mechanism to enhance or dampen the inflammatory response. CD26/dipeptidylpeptidase IV, ubiquitously expressed in tissues and blood, removes NH2-terminal dipeptides from proteins with a penultimate Pro or Ala. A large number of human chemokines, including CXCL2, CXCL6, CXCL9, CXCL10, CXCL11, CXCL12, CCL3L1, CCL4, CCL5, CCL11, CCL14, and CCL22, are cleaved by CD26; however, the efficiency is clearly influenced by the amino acids surrounding the cleavage site and although not yet proven, potentially affected by the chemokine concentration and interactions with third molecules...
June 2016: Journal of Leukocyte Biology
S Grad, C Bow, J Karppinen, K D Luk, K M Cheung, M Alini, D Samartzis
Lumbar disc degeneration severity on magnetic resonance imaging (MRI) is associated with low back pain. Pro-inflammatory chemokines CCL5 and CXCL6 are released by induced degenerative discs, and CCL5 has been associated with discogenic back pain. A case-control study was performed, based on the Hong Kong Disc Degeneration Population-Based Cohort of Southern Chinese, to investigate if systemic levels of CCL5 and CXCL6 were elevated in subjects with disc degeneration compared to non-degenerated individuals. Eighty subjects were selected, 40 with no disc degeneration (control group; DDD score 0) and 40 with moderate/severe disc degeneration (disc degeneration group; DDD score ≥5) as noted on MRI...
2016: European Cells & Materials
Ming-Yi Xu, Ying Qu, Zhenghong Li, Fei Li, Chun-Yang Xiao, Lun-Gen Lu
Clinical factors and liver biopsy cannot accurately predict the risk of developing cirrhosis in chronic hepatitis B (CHB).This study was to develop a predictive gene signature for cirrhosis in CHB patients. A total of 183 untreated CHB patients were enrolled. GeneChip, significant analysis of microarray (SAM) and prediction analysis of microarray (PAM) were used to select predictor genes (PGs) in liver tissues. The Cirrhosis Risk Score (CRS) was calculated based on 6 PG variables and the predictive value of CRS was evaluated...
2016: Frontiers in Bioscience (Landmark Edition)
Pei-Suen Tsou, Bradley J Rabquer, Ray A Ohara, William A Stinson, Phillip L Campbell, M Asif Amin, Beatrix Balogh, George Zakhem, Paul A Renauer, Ann Lozier, Eshwar Arasu, G Kenneth Haines, Bashar Kahaleh, Elena Schiopu, Dinesh Khanna, Alisa E Koch
OBJECTIVES: Angiogenesis plays a critical role in SSc (scleroderma). The aim of this study was to examine the expression of growth-regulated protein-γ (Gro-γ/CXCL3), granulocyte chemotactic protein 2 (GCP-2/CXCL6) and their receptor CXCR2 in endothelial cells (ECs) isolated from SSc skin and determine whether these cells mount an angiogenic response towards pro-angiogenic chemokines. The downstream signalling pathways as well as the pro-angiogenic transcription factor inhibitor of DNA-binding protein 1 (Id-1) were also examined...
April 2016: Rheumatology
Shimona Madalli, Martina Beyrau, James Whiteford, Johan Duchene, Inderpal Singh Nandhra, Nimesh S A Patel, Madhur P Motwani, Derek W Gilroy, Christoph Thiemermann, Sussan Nourshargh, Ramona S Scotland
BACKGROUND: Tissue infiltration by neutrophils during acute inflammatory states causes substantial tissue injury. While the magnitude of tissue neutrophil accumulation in innate immune responses is profoundly greater in males than females, fundamental aspects of the molecular mechanisms underlying these sex differences remain largely unknown. METHODS: We investigated sex differences in neutrophil stimulation and recruitment in ischemia/reperfusion (I/R; mesenteric or renal) or carrageenan pleurisy in rats or mice, as well as skin injury in human volunteers...
2015: Biology of Sex Differences
Susanne Schulz, Uta Dorothee Immel, Louise Just, Hans-Günter Schaller, Christiane Gläser, Stefan Reichert
BACKGROUND: Periodontitis is a chronic inflammatory disease triggered by the host immune response. Epigenetic modifications also affect the immune response. We assessed CpG methylation in 22 inflammatory candidate genes (ATF2, CCL25, CXCL14, CXCL3, CXCL5, CXCL6, FADD, GATA3, IL10RA, IL12A, IL12B, IL13, IL13RA1, IL15, IL17C, IL17RA, IL4R, IL6R, IL6ST, IL7, INHA, and TYK2) with respect to the occurrence of aggressive periodontitis (AgP). PATIENTS AND METHODS: In this study 15 AgP patients (53...
January 2016: Human Immunology
Markus Wolf, Markus Siegert, Simone Rothmiller, Nina Scheithauer, Romano Strobelt, Dirk Steinritz, Franz Worek, Horst Thiermann, Annette Schmidt
BACKGROUND: The cell line HaCaT/SM was derived from the human keratinocyte cell line HaCaT. HaCaT/SM cells display a high resistance against sulfur mustard (SM). Intention of the presented study was to determine the cellular and molecular differences between HaCaT/SM and HaCaT so as to evaluate which changes might be responsible for being resistant against SM. METHODS: Both cell lines HaCaT and HaCaT/SM were analyzed with respect to their cell growth, nuclei perimeter, clonogenicity and secretion profile...
February 26, 2016: Toxicology Letters
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