keyword
https://read.qxmd.com/read/38512049/single-cell-rna-sequencing-profiling-cellular-heterogeneity-and-specific-responses-of-fish-gills-to-microplastics-and-nanoplastics
#21
JOURNAL ARTICLE
Siwen Zheng, Wen-Xiong Wang
Fish gills are highly sensitive organs for microplastic (MP) and nanoplastic (NP) invasions, but the cellular heterogeneity of fish gills to MPs and NPs remains largely unknown. We employed single-cell RNA sequencing to investigate the responses of individual cell populations in tilapia Oreochromis niloticus gills to MP and NP exposure at an environmentally relevant concentration. Based on the detected differentially expressed gene (DEG) numbers, the most affected immune cells by MP exposure were macrophages, while the stimulus of NPs primarily targeted T cells...
March 21, 2024: Environmental Science & Technology
https://read.qxmd.com/read/38510108/yap-induces-a-neonatal-like-pro-renewal-niche-in-the-adult-heart
#22
JOURNAL ARTICLE
Rich Gang Li, Xiao Li, Yuka Morikawa, Francisco J Grisanti-Canozo, Fansen Meng, Chang-Ru Tsai, Yi Zhao, Lin Liu, Jong Kim, Bing Xie, Elzbieta Klysik, Shijie Liu, Md Abul Hassan Samee, James F Martin
After myocardial infarction (MI), mammalian hearts do not regenerate, and the microenvironment is disrupted. Hippo signaling loss of function with activation of transcriptional co-factor YAP induces heart renewal and rebuilds the post-MI microenvironment. In this study, we investigated adult renewal-competent mouse hearts expressing an active version of YAP, called YAP5SA, in cardiomyocytes (CMs). Spatial transcriptomics and single-cell RNA sequencing revealed a conserved, renewal-competent CM cell state called adult (a)CM2 with high YAP activity...
March 2024: Nat Cardiovasc Res
https://read.qxmd.com/read/38507713/effects-of-a-bioengineered-allogeneic-cellularized-construct-bacc-on-primary-human-macrophage-phenotype
#23
JOURNAL ARTICLE
Lindsay A Steele, Beatriz Hernaez Estrada, Kara L Spiller
The mechanisms behind the pro-healing effects of multicellular, bioengineered allogeneic cellularized constructs (BACC) are not known. Macrophages are key regulators of every phase of the wound healing process and are the primary cells that mediate the response to biomaterials. We hypothesized that cells within the BACC modulate macrophage behavior, which may contribute to the mechanism by which BACC promotes healing. To probe the influence of cells within the BACC compared to effects of the underlying collagen substrate, primary human macrophages were cultured in direct or indirect contact with BACC or with the same collagen substrate used in the BACC manufacturing...
March 20, 2024: Advanced Healthcare Materials
https://read.qxmd.com/read/38503922/human-lung-cancer-harbors-spatially-organized-stem-immunity-hubs-associated-with-response-to-immunotherapy
#24
JOURNAL ARTICLE
Jonathan H Chen, Linda T Nieman, Maxwell Spurrell, Vjola Jorgji, Liad Elmelech, Peter Richieri, Katherine H Xu, Roopa Madhu, Milan Parikh, Izabella Zamora, Arnav Mehta, Christopher S Nabel, Samuel S Freeman, Joshua D Pirl, Chenyue Lu, Catherine B Meador, Jaimie L Barth, Mustafa Sakhi, Alexander L Tang, Siranush Sarkizova, Colles Price, Nicolas F Fernandez, George Emanuel, Jiang He, Katrina Van Raay, Jason W Reeves, Keren Yizhak, Matan Hofree, Angela Shih, Moshe Sade-Feldman, Genevieve M Boland, Karin Pelka, Martin J Aryee, Mari Mino-Kenudson, Justin F Gainor, Ilya Korsunsky, Nir Hacohen
The organization of immune cells in human tumors is not well understood. Immunogenic tumors harbor spatially localized multicellular 'immunity hubs' defined by expression of the T cell-attracting chemokines CXCL10/CXCL11 and abundant T cells. Here, we examined immunity hubs in human pre-immunotherapy lung cancer specimens and found an association with beneficial response to PD-1 blockade. Critically, we discovered the stem-immunity hub, a subtype of immunity hub strongly associated with favorable PD-1-blockade outcome...
March 19, 2024: Nature Immunology
https://read.qxmd.com/read/38503010/biomimetic-nanoparticles-for-effective-celastrol-delivery-to-targeted-treatment-of-rheumatoid-arthritis-through-the-ros-nf-%C3%AE%C2%BAb-inflammasome-axis
#25
JOURNAL ARTICLE
Bo Wang, Jiquan Shen, Xinggao Wang, Ruixing Hou
Previous study has indicated that Celastrol (Cel) has various physiological and pharmacological effects, including antibacterial, antioxidant, pro-apoptotic, anticancer and anti-rheumatoid arthritis (RA) effects. However, low water solubility, low oral bioavailability, narrow treatment window, and high incidence of systemic adverse reactions still limit the further clinical application of Cel. Here, aiming at effectively overcome those shortcomings of Cel to boost its beneficial effects for treating RA, we developed the leukosome (LEUKO) coated biomimetic nanoparticles (NPs) for the targeted delivery of Cel to arthritis injury area in RA...
March 18, 2024: International Immunopharmacology
https://read.qxmd.com/read/38501838/combining-bulk-and-scrna-seq-to-explore-the-molecular-mechanisms-governing-the-distinct-efferocytosis-activities-of-a-macrophage-subpopulation-in-pdac
#26
JOURNAL ARTICLE
Shaoliang Zhu, Quan Cheng, Mengjie Zou, Chunxing Li, Yi Tang, Longjie Xia, Yanming Jiang, Zheng Gong, Zhenyong Tang, Yuntian Tang, Honglin Luo, Ningfu Peng, Xiaojing Wang, Xiaofeng Dong
Pancreatic ductal adenocarcinoma (PDAC), a very aggressive tumour, is currently the third leading cause of cancer-related deaths. Unfortunately, many patients face the issue of inoperability at the diagnostic phase leading to a quite dismal prognosis. The onset of metastatic processes has a crucial role in the elevated mortality rates linked to PDAC. Individuals with metastatic advances receive only palliative therapy and have a grim prognosis. It is essential to carefully analyse the intricacies of the metastatic process to enhance the prognosis for individuals with PDAC...
April 2024: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/38501437/fibroblast-activation-protein-promotes-progression-of-hepatocellular-carcinoma-via-regulating-the-immunity
#27
JOURNAL ARTICLE
Xiangcheng Wang, Ruilong Niu, Hao Yang, Yu Lin, Hui Hou, Hong Yang
Fibroblast activation protein (FAP) has been indicated to express in cancer-associated fibroblasts (CAFs) in most cancers. This work was dedicated to exploring FAP's effects on hepatocellular carcinoma (HCC). The data were extracted from The Cancer Genome Atlas, Gene Expression Omnibus, ImmPort, and Reactome databases. The correlation between FAP and HCC patients' prognosis was explored via survival analysis. The qRT-PCR and western blot analysis were used to analyze the FAP mRNA and protein expression levels, respectively...
March 19, 2024: Cell Biology International
https://read.qxmd.com/read/38499092/a-supramolecular-hydrogel-dressing-with-antibacterial-immunoregulation-and-pro-regeneration-ability-for-biofilm-associated-wound-healing
#28
JOURNAL ARTICLE
Yumeng Wang, Xinghong Zhao, Xingjian Zhou, Juqin Dai, Xiaowen Hu, Yinzi Piao, Guangyue Zu, Jian Xiao, Keqing Shi, Yong Liu, Yuanfeng Li, Linqi Shi
Chronic wound treatment has emerged as a significant healthcare concern worldwide due to its substantial economic burden and the limited effectiveness of current treatments. Effective management of biofilm infections, regulation of excessive oxidative stress, and promotion of tissue regeneration are crucial for addressing chronic wounds. Hydrogel stands out as a promising candidate for chronic wound treatment. However, its clinical application is hindered by the difficulty in designing and fabricating easily and conveniently...
March 16, 2024: Journal of Controlled Release
https://read.qxmd.com/read/38498906/the-comprehensive-landscape-of-prognosis-immunity-and-function-of-the-gli-family-by-pan-cancer-and-single-cell-analysis
#29
JOURNAL ARTICLE
Yinteng Wu, Wenliang Guo, Tao Wang, Ying Liu, Marìa Del Mar Requena Mullor, Raquel Alarcòn Rodrìguez, Shijian Zhao, Ruqiong Wei
The Hedgehog (Hh) signaling pathway has been implicated in the pathogenesis of various cancers. However, the roles of the downstream GLI family (GLI1, GLI2, and GLI3) in tumorigenesis remain elusive. This study aimed to unravel the genetic alterations of GLI1/2/3 in cancer and their association with the immune microenvironment and related signaling pathways. Firstly, we evaluated the expression profiles of GLI1/2/3 in different cancer types, analyzed their prognostic and predictive values, and assessed their correlation with tumor-infiltrating immune cells...
March 18, 2024: Aging
https://read.qxmd.com/read/38493215/axl-and-mertk-regulate-synovial-inflammation-and-are-modulated-by-il-6-inhibition-in-rheumatoid-arthritis
#30
JOURNAL ARTICLE
Alessandra Nerviani, Marie-Astrid Boutet, Giulia Maria Ghirardi, Katriona Goldmann, Elisabetta Sciacca, Felice Rivellese, Elena Pontarini, Edoardo Prediletto, Federico Abatecola, Mattia Caliste, Sara Pagani, Daniele Mauro, Mattia Bellan, Cankut Cubuk, Rachel Lau, Sarah E Church, Briana M Hudson, Frances Humby, Michele Bombardieri, Myles J Lewis, Costantino Pitzalis
The TAM tyrosine kinases, Axl and MerTK, play an important role in rheumatoid arthritis (RA). Here, using a unique synovial tissue bioresource of patients with RA matched for disease stage and treatment exposure, we assessed how Axl and MerTK relate to synovial histopathology and disease activity, and their topographical expression and longitudinal modulation by targeted treatments. We show that in treatment-naive patients, high AXL levels are associated with pauci-immune histology and low disease activity and inversely correlate with the expression levels of pro-inflammatory genes...
March 16, 2024: Nature Communications
https://read.qxmd.com/read/38492583/clinical-therapeutics-for-proliferative-vitreoretinopathy-in-retinal-detachment
#31
REVIEW
Francesco Sabatino, Philip Banerjee, Mahiul M K Muqit
Proliferative vitreoretinopathy (PVR) is an abnormal and prolonged healing response to retinal injury (retinal detachment, post-retinal detachment surgery) characterised by: pre/subretinal membrane formation; retinal gliosis and retinal shortening, retinal pigment epithelium cell proliferation; and increased glial (mainly Mu¨ller cells), fibroblast and inflammatory cell (macrophage, lymphocyte) activity, leading to tractional retinal holes/breaks and multiple costly eye operations suffered by patients...
March 14, 2024: Survey of Ophthalmology
https://read.qxmd.com/read/38492336/interleukin-1%C3%AE-polarization-in-m1-macrophage-mediates-myocardial-fibrosis-in-diabetes
#32
JOURNAL ARTICLE
Wenli Guo, Chen Yang, Jiawei Zou, Tingting Yu, Mingde Li, Ruilin He, Keyang Chen, Rafaela C R Hell, Eric R Gross, Xin Zou, Yao Lu
BACKGROUND: Diabetes is a global health problem whose common complication is diabetic cardiomyopathy, characterized by chronic inflammation of the heart muscle. Macrophages are the main white blood cells found in the resting heart. Therefore, we investigated the underling mechanism of macrophage on myocardial fibrosis in diabetes. METHODS: Here, echocardiography was utilized to evaluate cardiac function, and the degree of myocardial fibrosis was assessed using Masson's trichrome staining, followed by single-cell RNA sequencing (scRNA-seq) to analyze the phenotype, function, developmental trajectory, and interactions between immune cells, endothelial cells (ECs), and fibroblasts (FBs) in the hearts of db/db mice at different stages of diabetes...
March 15, 2024: International Immunopharmacology
https://read.qxmd.com/read/38490958/proteomic-analysis-of-ascitic-extracellular-vesicles-describes-tumour-microenvironment-and-predicts-patient-survival-in-ovarian-cancer
#33
JOURNAL ARTICLE
Anna Vyhlídalová Kotrbová, Kristína Gömöryová, Antónia Mikulová, Hana Plešingerová, Stanislava Sladeček, Marek Kravec, Šárka Hrachovinová, David Potěšil, Garett Dunsmore, Camille Blériot, Mathilde Bied, Jan Kotouček, Markéta Bednaříková, Jitka Hausnerová, Luboš Minář, Igor Crha, Michal Felsinger, Zbyněk Zdráhal, Florent Ginhoux, Vít Weinberger, Vitězslav Bryja, Vendula Pospíchalová
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type of ovarian cancer, ranks among the deadliest malignancies. Many HGSC patients have excess fluid in the peritoneum called ascites. Ascites is a tumour microenvironment (TME) containing various cells, proteins and extracellular vesicles (EVs). We isolated EVs from patients' ascites by orthogonal methods and analyzed them by mass spectrometry. We identified not only a set of 'core ascitic EV-associated proteins' but also defined their subset unique to HGSC ascites...
March 2024: Journal of Extracellular Vesicles
https://read.qxmd.com/read/38489548/single-dual-specific-anti-pd-l1-tgf-%C3%AE-antibody-synergizes-with-chemotherapy-as-neoadjuvant-treatment-for-pancreatic-ductal-adenocarcinoma-a-preclinical-experimental-study
#34
JOURNAL ARTICLE
Haoxiang Zhang, Jiaoshun Chen, Jianwei Bai, Jing Zhang, Shaoyi Huang, Liang Zeng, Pengfei Zhou, Qiang Shen, Tao Yin
AIMS: Chemotherapy resistance is an important cause of neoadjuvant therapy failure in pancreatic ductal adenocarcinoma (PDAC). BiTP is a single antibody that can simultaneously and dually target transforming growth factor-beta (TGF-β) and programmed cell death 1 ligand 1 (PD-L1). We attempted in this study to investigate the efficacy of BiTP in combination with first-line chemotherapy in PDAC. METHODS: Preclinical assessments of BiTP plus gemcitabine and nab-paclitaxel were completed through a resectable KPC mouse model (C57BL/6J)...
March 14, 2024: International Journal of Surgery
https://read.qxmd.com/read/38488753/leukemia-inhibitory-factor-protects-against-experimental-periodontitis-through-immuno-modulations-of-both-macrophages-and-periodontal-ligament-fibroblasts
#35
JOURNAL ARTICLE
Yanjing Ou, Le Fan, Xiaoqi Wang, Haibin Xia, Mengwen Cheng, Jing Huang, Youde Liang, Yining Wang, Yi Zhou
BACKGROUND: To explore the role of leukemia inhibitory factor (LIF) in periodontitis via in vivo and in vitro experiments. METHODS: The second upper molar of LIF knockout mice and their wild-type littermates were ligated for 8 days. Micro-computed tomography (micro-CT), histological analysis, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed. The expression levels of proinflammatory cytokines were examined in mouse bone marrow derived macrophages and human periodontal ligament fibroblasts (HPDLFs) after lipopolysaccharide (LPS) treatment...
March 15, 2024: Journal of Periodontology
https://read.qxmd.com/read/38488272/immunohistochemical-analysis-of-chronic-and-recurrent-dermatophytosis
#36
JOURNAL ARTICLE
Ramesh M Bhat, Monisha Madhumita, Nisha J Marla, Jyothi Jayaraman
BACKGROUND: Dermatophytosis has assumed epidemic proportions with rising resistance, recalcitrance and recurrence, especially in tropical regions. While various factors contribute to high prevalence worldwide, yet little is known about the interactions between host defence mechanisms and dermatophytes, particularly in chronic and recalcitrant dermatophytosis. OBJECTIVES: We aimed to compare the population of various immune cells in specimens of chronic recurrent dermatophytosis and those with acute superficial dermatophytosis...
March 2024: Mycoses
https://read.qxmd.com/read/38487879/small-extracellular-vesicles-from-infarcted-and-failing-heart-accelerate-tumor-growth
#37
JOURNAL ARTICLE
Tal Caller, Itai Rotem, Olga Shaihov-Teper, Daria Lendengolts, Yeshai Schary, Ruty Shai, Efrat Glick-Saar, Dan Dominissini, Menachem Motiei, Idan Katzir, Rachela Popovtzer, Merav Nahmoud, Alex Boomgarden, Crislyn D'Souza-Schorey, Nili Naftali-Shani, Jonathan Leor
BACKGROUND: Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. However, the mechanism is complex and unclear. Here, we aimed to test our hypothesis that cardiac small extracellular vesicles (sEVs), particularly cardiac mesenchymal stromal cell-derived sEVs (cMSC-sEVs), contribute to the link between post-MI left ventricular dysfunction (LVD) and cancer. METHODS: We purified and characterized sEVs from post-MI hearts and cultured cMSCs...
March 15, 2024: Circulation
https://read.qxmd.com/read/38486384/bioactive-fractions-isolated-from-harungana-madagascariensis-lam-and-psorospermum-aurantiacum-engl-regulate-collagen-and-melanin-biosynthesis-gene-expression-in-uvb-irradiated-cells-with-additional-anti-inflammatory-potential
#38
JOURNAL ARTICLE
Jacqueline N Manjia, Apeksha Joshi, Emmanuel Mfotie Njoya, Kapil Upadhyay, Corinne Ngnameko, Lyndy J McGaw, Ranjitsinh Devkar, Frederic N Njayou, Paul F Moundipa
BACKGROUND: Harungana madagascariensis (HM) and Psorospermum aurantiacum (PA), used traditionally for skin care, have been reported to upregulate the expression of intracellular antioxidant genes, thereby preventing melanoma and protecting fibroblast cell lines from Ultraviolet B (UVB)-induced intracellular oxidative stress. AIMS: This investigation aimed to identify major compounds in bioactive fractions using bioassay- guided fractionation. METHODS: The anti-inflammatory effect of fractions was determined by measuring their inhibitory activity on 15-lipoxygenase and nitric oxide (NO) in lipopolysaccharide-stimulated RAW 264...
March 13, 2024: Current Drug Research Reviews
https://read.qxmd.com/read/38485100/macrophage-phenotype-is-determinant-for-fibrosis-development-in-keloid-disease
#39
JOURNAL ARTICLE
Zélie Dirand, Mélissa Maraux, Marion Tissot, Brice Chatelain, Dorothy Supp, Céline Viennet, Sylvain Perruche, Gwenaël Rolin
Keloid refers to a fibroproliferative disorder characterized by an accumulation of extracellular matrix (ECM) components at the dermis level, overgrowth beyond initial wound, and formation of tumor-like nodule areas. Treating keloid is still an unmet clinical need and the lack of an efficient therapy is clearly related to limited knowledge about keloid etiology, despite the growing interest of the scientific community in this pathology. In past decades, keloids were often studied in vitro through the sole prism of fibroblasts considered as the major effector of ECM deposition...
March 12, 2024: Matrix Biology: Journal of the International Society for Matrix Biology
https://read.qxmd.com/read/38484831/ph-responsive-size-adjustable-liposomes-induce-apoptosis-of-fibroblasts-and-macrophages-for-rheumatoid-arthritis-treatment
#40
JOURNAL ARTICLE
Xiu Zheng, Hong Yang, Zongquan Zhang, Xiaoya Liang, Yan Liu, Chenglong Wang, Xi Yang, Jun Tang, Jingying Mao, Yu Nie, Xiangyu Zhou, Chunhong Li
In rheumatoid arthritis (RA), macrophages infiltrate joints, while fibroblast-like synovial cells proliferate abnormally, forming a barrier against drug delivery, which hinders effective drug delivery to joint focus. Here we firstly designed a pH-responsive size-adjustable nanoparticle, composed by methotrexate (MTX)-human serum albumin (HSA) complex coating with pH-responsive liposome (Lipo/MTX-HSA) for delivering drugs specifically to inflamed joints in acidic environments. We showed in vitro that the nanoparticles can induce mitochondrial dysfunction, promote apoptosis of fibroblast-like synoviocytes and macrophages, further reduce the secretion of inflammatory factors (TNF-α, IL-1β, MMP-9), and regulate the inflammatory microenvironment...
March 12, 2024: Acta Biomaterialia
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