Nienke J T van Kooten, Arjen B Blom, Iris J Teunissen van Manen, Wessel F Theeuwes, Johannes Roth, Mark A J Gorris, Birgitte Walgreen, Annet W Sloetjes, Monique M Helsen, Elly L Vitters, Peter L E M van Lent, Sander Koëter, Peter M van der Kraan, Thomas Vogl, Martijn H J van den Bosch
OBJECTIVES: Macrophages are key orchestrators of the osteoarthritis (OA)-associated inflammatory response. Macrophage phenotype is dependent on environmental cues like the inflammatory factor S100A8/A9. Here, we investigated how S100A9 exposure during monocyte-to-macrophage differentiation affects macrophage phenotype and function. METHODS: OA synovium cellular composition was determined using flow cytometry and multiplex immunohistochemistry. Healthy donor monocytes were differentiated towards M1- and M2-like macrophages in presence of S100A9...
January 12, 2024: Rheumatology