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https://www.readbyqxmd.com/read/28445599/growth-engineering-of-synechococcus-elongatus-pcc-7942-for-mixotrophy-under-natural-light-conditions-for-improved-feedstock-production
#1
Aditya Sarnaik, Reena Pandit, Arvind Lali
Synechococcus elongatus PCC 7942 has been widely explored as cyanobacterial cell factory through genetic modifications for production of various value-added compounds. However, successful industrial scale-ups have not been reported for the system predominantly due to its obligate photoautotrophic metabolism and use of artificial light in photobioreactors. Hence, engineering the organism to perform mixotrophy under natural light could serve as an effective solution. Thus, we applied a genetically engineered strain of Synechococcus elongatus PCC 7942 expressing heterologous hexose transporter gene (galP) to perform mixotrophy under natural light in a temperature controlled environmental chamber (EC)...
April 26, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28442456/the-malaria-system-microapp-a-new-mobile-device-based-tool-for-malaria-diagnosis
#2
Allisson Dantas Oliveira, Clara Prats, Mateu Espasa, Francesc Zarzuela Serrat, Cristina Montañola Sales, Aroa Silgado, Daniel Lopez Codina, Mercia Eliane Arruda, Jordi Gomez I Prat, Jones Albuquerque
BACKGROUND: Malaria is a public health problem that affects remote areas worldwide. Climate change has contributed to the problem by allowing for the survival of Anopheles in previously uninhabited areas. As such, several groups have made developing news systems for the automated diagnosis of malaria a priority. OBJECTIVE: The objective of this study was to develop a new, automated, mobile device-based diagnostic system for malaria. The system uses Giemsa-stained peripheral blood samples combined with light microscopy to identify the Plasmodium falciparum species in the ring stage of development...
April 25, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28440636/probing-and-comparing-the-photobromination-and-photoiodination-of-dissolved-organic-matter-by-using-ultrahigh-resolution-mass-spectrometry
#3
Zhineng Hao, Yongguang Yin, Dong Cao, Jing-Fu Liu
Photochemical halogenation of dissolved organic matter (DOM) may represent an important abiotic source of natural organobromine compounds (OBCs) and natural organoiodine compounds (OICs) within surface waters. Here we report the significantly enhanced formation of OBCs and OICs by photohalogenating DOM in freshwater and seawater, as well as the noticeable difference in distribution and composition pattern of newly formed OBCs and OICs. By using the negative ion electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry, numerous OBCs and OICs were identified during the photo halogenation processes in sunlit waters...
April 25, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28432328/tactile-flow-overides-other-cues-to-self-motion
#4
Laurence R Harris, Kenzo Sakurai, William H A Beaudot
Vestibular-somatosensory interactions are pervasive in the brain but it remains unclear why. Here we explore the contribution of tactile flow to processing self-motion. We assessed two aspects of self-motion: timing and speed. Participants sat on an oscillating swing and either kept their hands on their laps or rested them lightly on an earth-stationary surface. They viewed a grating oscillating at the same frequency as their motion and judged its phase or, in a separate experiment, its speed relative to their perceived motion...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425557/dual-spectra-band-emissive-eu-2-mn-2-co-activated-alkaline-earth-phosphates-for-indoor-plant-growth-novel-phosphor-converted-leds
#5
Young Jun Yun, Jin Kyu Kim, Ji Young Ju, Seul Ki Choi, Woon Ik Park, Jae Yong Suh, Ha-Kyun Jung, Yongseon Kim, Sungho Choi
This paper reports designing a novel single composition blue/red color illuminating phosphor followed by fabricating "smart" agricultural/horticultural LED lighting. Color-tunable Eu(2+)/Mn(2+) co-activated alkaline earth phosphates, Na(Sr,Ba)PO4 and Ca3Mg3(PO4)4, are considered, and the stable doping sites for the corresponding activators are identified by using first-principle DFT calculations. We can realize the designated color purity with stable thermal quenching preserved luminescence behavior is induced by the Eu(2+) center positioned at different coordination states with intermixed Sr(2+)/Ba(2+) sites in Na(Sr,Ba)PO4 hosts...
April 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28420077/recognition-of-the-duration-and-prediction-of-insect-prevalence-of-stored-rough-rice-infested-by-the-red-flour-beetle-tribolium-castaneum-herbst-using-an-electronic-nose
#6
Sai Xu, Zhiyan Zhou, Keliang Li, Sierra Mari Jamir, Xiwen Luo
The purpose of this research is to explore the feasibility of applying an electronic nose for the intelligent monitoring of injurious insects in a stored grain environment. In this study, we employed an electronic nose to sample rough rice that contained three degrees of red flour beetle (Tribolium castaneum Herbst) infestation for different durations-light degree (LD), middle degree (MD), and heavy degree (HD)-and manually investigated the insect situation at the same time. Manual insect situation investigation shows that, in all three rice treatments, the insect amounts gradually decreased after infestation...
April 14, 2017: Sensors
https://www.readbyqxmd.com/read/28419477/the-effectiveness-of-riboflavin-and-ultraviolet-light-pathogen-reduction-technology-in-eliminating-trypanosoma-cruzi-from-leukoreduced-whole-blood
#7
Teresa Jimenez-Marco, Beatriz Cancino-Faure, Enrique Girona-Llobera, M Magdalena Alcover, Cristina Riera, Roser Fisa
BACKGROUND: The parasitic Chagas disease is caused by the protozoan Trypanosoma cruzi, which is mainly transmitted by insect vectors. Other infection routes, both in endemic and in nonendemic areas, include organ and marrow transplantation, congenital transmission, and blood transfusion. Asymptomatic chronic chagasic individuals may have a low and transient parasitemia in peripheral blood and, consequently, they can unknowingly transmit the disease via blood transfusion. Riboflavin and ultraviolet (UV) light pathogen reduction is a method to reduce pathogen transfusion transmission risk based on damage to the pathogen nucleic acids...
April 16, 2017: Transfusion
https://www.readbyqxmd.com/read/28419148/how-long-to-rest-in-unpredictably-changing-habitats
#8
Mirosław Slusarczyk, Jacek Starzyński, Piotr Bernatowicz
In the present study, we investigated the optimum length of prolonged dormancy (developmental arrest extending over favourable periods) of organisms under uncertain environmental conditions. We used an artificial life model to simulate the evolution of suspended development in the ontogenesis of organisms inhabiting unpredictably changing habitats. A virtual population of semelparous parthenogenetic individuals that varied in a duration of developmental arrest competed for limited resources. At a constant level of available resources, uninterrupted development was the superior life strategy...
2017: PloS One
https://www.readbyqxmd.com/read/28418640/artificially-smart-vesicles-with-superior-structural-stability-fabrication-characterizations-and-transmembrane-traffic
#9
Wen-Jian Zhang, Chun-Yan Hong, Cai-Yuan Pan
Intelligent vesicles are fabricated at up to 30% solid content via an approach of polymerization-induced self-assembly and reorganization (PISR). Upon irradiation with UV light (365 nm), light-triggered dimerization of the coumarin moieties anchored in the membrane leads to the membrane cross-linking of the vesicles, which endows the vesicles with superior structural stability. Due to the tertiary amine groups in the membrane, the vesicles go through a swelling/deswelling change upon switching the pH values...
April 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28418080/phototoxic-potential-of-undispersed-and-dispersed-fresh-and-weathered-macondo-crude-oils-to-gulf-of-mexico-marine-organisms
#10
Bryson E Finch, Solmaz Marzooghi, Dominic M Di Toro, William A Stubblefield
Crude oils contain a mixture of hydrocarbons, including phototoxic polycyclic aromatic hydrocarbons (PAHs) that have the ability to absorb ultraviolet (UV) light. Absorption of UV light by PAHs can substantially increase their toxicity to marine organisms. The objective of the present study was to examine the potential for phototoxicity of fresh and naturally weathered Macondo crude oils alone and in combination with the dispersant Corexit 9500 to mysid shrimp (Americamysis bahia), inland silverside (Menidia beryllina), sheepshead minnow (Cyprinodon variegatus), and Gulf killifish (Fundulus grandis)...
April 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28416382/isolation-pathogenicity-and-characterization-of-a-novel-bacterial-pathogen-streptococcus-uberis-from-diseased-mandarin-fish-siniperca-chuatsi
#11
Xia Luo, Xiaozhe Fu, Guoli Liao, Ouqin Chang, Zhibin Huang, Ningqiu Li
In recent years, mandarin fish had a high mortality rate associated with abnormal swimming, exophthalmia, corneal opacity and eye hemorrhage on a fish farm located at Foshan city, Guangdong province, China. Three isolates of Gram-positive, chain-forming cocci were recovered from moribund fish, and designated as SS131025-1, SS131025-2, and SS131025-3. These isolates were identified as Streptococcus uberis according to their morphologic and physio-biochemical characteristics as well as phylogenetic analysis based on their 16S rRNA and GapC gene sequences...
April 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28415539/in-vitro-cytotoxicity-evaluation-of-nano-carbon-particles-with-different-sp-2-sp-3-ratios
#12
S S Li, B J Wu, Q Y Deng, Y B Guo, Y X Leng, N Huang
Graphitization occurs during the long-term service of a diamond-like carbon (DLC) modified artificial joint. Then, DLC wear debris, which are carbon particles with different sp(2)/sp(3) ratios and sizes ranging from the nano- to micro-meter scale produced. In this paper, to promote the application of DLC coating for artificial joint modification, the cytotoxicity of DLC debris (nano-carbon particles, NCs) with different sp(2)/sp(3) ratios was studied. The microstructure and physical characteristics of NCs with different sp(2)/sp(3) ratios were investigated by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415286/dissociation-between-two-subgroups-of-the-suprachiasmatic-nucleus-affected-by-the-number-of-damped-oscillated-neurons
#13
Changgui Gu, Huijie Yang, Jos Ht Rohling
In mammals, the main clock located in the suprachiasmatic nucleus (SCN) of the brain synchronizes the body rhythms to the environmental light-dark cycle. The SCN is composed of about 2×10^{4} neurons which can be classified into three oscillatory phenotypes: self-sustained oscillators, damped oscillators, and arrhythmic neurons. Exposed to an artificial external light-dark cycle with a period of 22h instead of 24h, two subgroups of the SCN can become desynchronized (dissociated). The ventrolateral (VL) subgroup receives photic input and is entrained to the external cycle and a dorsomedial (DM) subgroup oscillates with its endogenous (i...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28414344/measurement-of-thickness-of-highly-inhomogeneous-crude-oil-slicks
#14
Surya Cheemalapati, Heather P Forth, Hao Wang, Karthik Raj Konnaiyan, Jeffrey M Morris, Anna L Pyayt
As part of the Deepwater Horizon toxicity testing program, a number of laboratories generated oil slicks in the laboratory to study potential toxic effects of these oil slicks on aquatic organisms. Understanding the details of how these slicks affect aquatic organisms requires careful correlation between slick thickness and the observed detrimental effects. Estimating oil film thickness on water can be challenging since the traditional color-based technique used in the field is very imprecise. Also, as we demonstrate here, the films formed on the water surface are highly nonuniform on a microscale level, and thus uniform thin film thickness measurement techniques based on optical interference do not work...
April 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/28411604/ultrafast-coherent-energy-transfer-with-high-efficiency-based-on-plasmonic-nanostructures
#15
Jun Ren, Tian Chen, Bo Wang, Xiangdong Zhang
The theory of energy transfer dynamics of a pair of donor and acceptor molecules located in the plasmonic hot spots is developed by means of the master equation approach and the electromagnetic Green's tensor technique. A nonlocal effect has been considered by using a hydrodynamic model. The coherent interaction between the two molecules in plasmonic nanostructures is investigated, and we find that the coupling strength between two molecules can be larger than dissipation. It is shown that the energy transfer efficiency of a pair of molecules can be improved largely and the transfer time decreases to dozens of femtoseconds when the contribution of quantum coherence is considered...
April 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28410120/snp_tata_comparator-genomewide-landmarks-for-preventive-personalized-medicine
#16
Mikhail Ponomarenko, Dmitry Rasskazov, Irina Chadaeva, Ekaterina Sharypova, Petr Ponomarenko, Olga Arkova, Elena Kashina, Nikita Ivanisenko, Dmitry Zhechev, Ludmila Savinkova, Nikolay Kolchanov
Year after year, conditions, quality, and duration of human lives have been improving due to the progress of science, technology, education, and medicine, which however has a downside. Owing to improvement in children's nutrition, developmental acceleration occurs that imbalances a child's system. Because of virtual worlds of the Internet, social experience of teenagers expands and clashes with puberty of adolescents. Due to the comfort of cities, urbanization emerges and causes stress to adults because of artificial light, noise, pollution, violations of personal space, and family disruption...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28409386/nocturnal-oviposition-behavior-of-blowflies-diptera-calliphoridae-in-the-southern-hemisphere-south-africa-and-australia-and-its-forensic-implications
#17
Kirstin A Williams, James F Wallman, Bryan D Lessard, Christopher R J Kavazos, D Nkosinathi Mazungula, Martin H Villet
Published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minPMI) by up to 12 hours, depending on latitude and season. The majority of published studies are from the northern hemisphere. Field experiments were conducted in South Africa and Australia that extend observations to species of the southern hemisphere. Various vertebrate carrion was exposed at night in summer under different lunar phases and/or artificial lighting, and in woodland and pasture areas...
April 13, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28406968/the-sense-of-balance-in-humans-structural-features-of-otoconia-and-their-response-to-linear-acceleration
#18
Rüdiger Kniep, Dirk Zahn, Jana Wulfes, Leif Erik Walther
We explored the functional role of individual otoconia within the otolith system of mammalians responsible for the detection of linear accelerations and head tilts in relation to the gravity vector. Details of the inner structure and the shape of intact human and artificial otoconia were studied using environmental scanning electron microscopy (ESEM), including decalcification by ethylenediaminetetraacetic acid (EDTA) to discriminate local calcium carbonate density. Considerable differences between the rhombohedral faces of human and artificial otoconia already indicate that the inner architecture of otoconia is not consistent with the point group -3m...
2017: PloS One
https://www.readbyqxmd.com/read/28406471/real-time-vision-based-road-sign-recognition-using-an-artificial-neural-network
#19
Kh Tohidul Islam, Ram Gopal Raj
Road sign recognition is a driver support function that can be used to notify and warn the driver by showing the restrictions that may be effective on the current stretch of road. Examples for such regulations are 'traffic light ahead' or 'pedestrian crossing' indications. The present investigation targets the recognition of Malaysian road and traffic signs in real-time. Real-time video is taken by a digital camera from a moving vehicle and real world road signs are then extracted using vision-only information...
April 13, 2017: Sensors
https://www.readbyqxmd.com/read/28406466/artificial-red-blood-cells-as-potential-photosensitizers-in-dye-laser-treatment-against-port-wine-stains
#20
REVIEW
Naoaki Rikihisa, Shoji Watanabe, Yoshiaki Saito, Hiromi Sakai
We suggest a novel method that uses artificial blood cells (hemoglobin vesicles, Hb-Vs) as photosensitizers in dye laser treatment (at 595-nm wavelength) for port-wine stains (i.e., capillary malformations presenting as red birthmarks) based on the results of animal experiments. As compared with human red blood cells, Hb-Vs have the same absorbance of 595 nm wavelength light and produce the same level of heat following dye laser irradiation. Small sized Hb-Vs (250 nm) distribute in the plasma phase in blood and tend to flow in the marginal zone of microvessels...
April 13, 2017: Journal of Functional Biomaterials
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