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https://www.readbyqxmd.com/read/29777691/improving-electrical-properties-of-ipsc-cardiomyocytes-by-enhancing-cx43-expression
#1
Valentin Sottas, Carl-Mattheis Wahl, Mihnea C Trache, Michael Bartolf-Kopp, Sidney Cambridge, Markus Hecker, Nina D Ullrich
The therapeutic potential of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by immature functional features including low impulse propagation and reduced cell excitability. Key players regulating electrical activity are voltage-gated Na+ channels (Nav 1.5) and gap junctions built from connexin-43 (Cx43). Here we tested the hypothesis that enhanced Cx43 expression increases intercellular coupling and influences excitability by modulating Nav 1.5. Using transgenic approaches, Cx43 and Nav 1...
May 16, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29751502/the-development-of-compartmentation-of-camp-signaling-in-cardiomyocytes-the-role-of-t-tubules-and-caveolae-microdomains
#2
REVIEW
Navneet K Bhogal, Alveera Hasan, Julia Gorelik
3′-5′-cyclic adenosine monophosphate (cAMP) is a signaling messenger produced in response to the stimulation of cellular receptors, and has a myriad of functional applications depending on the cell type. In the heart, cAMP is responsible for regulating the contraction rate and force; however, cAMP is also involved in multiple other functions. Compartmentation of cAMP production may explain the specificity of signaling following a stimulus. In particular, transverse tubules (T-tubules) and caveolae have been found to be critical structural components for the spatial confinement of cAMP in cardiomyocytes, as exemplified by beta-adrenergic receptor (β-ARs) signaling...
May 3, 2018: Journal of Cardiovascular Development and Disease
https://www.readbyqxmd.com/read/29747586/selective-modulation-of-local-linkages-between-active-transcription-and-oxidative-demethylation-activity-shapes-cardiomyocyte-specific-gene-body-epigenetic-status-in-mice
#3
Mayumi Oda, Shunichi Wakabayashi, N Ari Wijetunga, Shinsuke Yuasa, Hirokazu Enomoto, Ruri Kaneda, Sung Han Yoon, Nishant Mittal, Qiang Jing, Masako Suzuki, John M Greally, Keiichi Fukuda, Shinji Makino
BACKGROUND: Cell-type-specific genes exhibit heterogeneity in genomic contexts and may be subject to different epigenetic regulations through different gene transcriptional processes depending on the cell type involved. The gene-body regions (GBRs) of some cardiomyocyte (CM)-specific genes are long and highly hypomethylated in CMs. To explore the cell-type specificities of epigenetic patterns and functions, multiple epigenetic modifications of GBRs were compared among CMs, liver cells and embryonic stem cells (ESCs)...
May 10, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29743935/pharmacokinetics-of-chinese-medicines-strategies-and-perspectives
#4
REVIEW
Ru Yan, Ying Yang, Yijia Chen
The modernization and internationalization of Chinese medicines (CMs) are hampered by increasing concerns on the safety and the efficacy. Pharmacokinetic (PK) study is indispensable to establish concentration-activity/toxicity relationship and facilitate target identification and new drug discovery from CMs. To cope with tremendous challenges rooted from chemical complexity of CMs, the classic PK strategies have evolved rapidly from PK study focusing on marker/main drug components to PK-PD correlation study adopting metabolomics approaches to characterize associations between disposition of global drug-related components and host metabolic network shifts...
2018: Chinese Medicine
https://www.readbyqxmd.com/read/29731429/electrophysiologic-characterization-of-calcium-handling-in-human-induced-pluripotent-stem-cell-derived-atrial-cardiomyocytes
#5
Mariana Argenziano, Erin Lambers, Liang Hong, Arvind Sridhar, Meihong Zhang, Brandon Chalazan, Ambili Menon, Eleonora Savio-Galimberti, Joseph C Wu, Jalees Rehman, Dawood Darbar
Human induced pluripotent stem cell (hiPSC)-derived atrial cardiomyocytes (CMs) hold great promise for elucidating underlying cellular mechanisms that cause atrial fibrillation (AF). In order to use atrial-like hiPSC-CMs for arrhythmia modeling, it is essential to better understand the molecular and electrophysiological phenotype of these cells. We performed comprehensive molecular, transcriptomic, and electrophysiologic analyses of retinoic acid (RA)-guided hiPSC atrial-like CMs and demonstrate that RA results in differential expression of genes involved in calcium ion homeostasis that directly interact with an RA receptor, chicken ovalbumin upstream promoter-transcription factor 2 (COUP-TFII)...
April 28, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29720615/characterizing-the-role-of-atrial-natriuretic-peptide-signaling-in-the-development-of-embryonic-ventricular-conduction-system
#6
Arun Govindapillai, Adam Hotchkiss, Mark Baguma-Nibasheka, Robert A Rose, Lucile Miquerol, Oliver Smithies, Nobuyo Maeda, Kishore B S Pasumarthi
Patients born with congenital heart defects frequently encounter arrhythmias due to defects in the ventricular conduction system (VCS) development. Although recent studies identified transcriptional networks essential for the heart development, there is scant information on the mechanisms regulating VCS development. Based on the association of atrial natriuretic peptide (ANP) expression with VCS forming regions, it was reasoned that ANP could play a critical role in differentiation of cardiac progenitor cells (CPCs) and cardiomyocytes (CMs) toward a VCS cell lineage...
May 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29716942/mechanosensitive-gene-regulation-by-myocardin-related-transcription-factors-is-required-for-cardiomyocyte-integrity-in-load-induced-ventricular-hypertrophy
#7
Michael A Trembley, Pearl Quijada, Esperanza Agullo-Pascual, Kevin M Tylock, Mert Colpan, Ronald A Dirkx, Jason R Myers, Deanne M Mickelsen, Karen de Mesy Bentley, Eli Rothenberg, Christine S Moravec, Jeffrey D Alexis, Carol C Gregorio, Robert T Dirksen, Mario Delmar, Eric M Small
Background -Hypertrophic cardiomyocyte (CM) growth and dysfunction accompanies various forms of heart disease. The mechanisms responsible for transcriptional changes that impact cardiac physiology and the transition to heart failure (HF) are not well understood. The intercalated disc (ID) is a specialized intercellular junction coupling CM electrical activity and force transmission, and is gaining attention as a mechanosensitive signaling hub and hotspot for causative mutations in cardiomyopathy. Methods -Transmission electron microscopy, confocal microscopy, and single-molecule localization microscopy (SMLM) were used to examine changes in ID structure and protein localization in the murine and human heart...
May 1, 2018: Circulation
https://www.readbyqxmd.com/read/29713785/regulation-of-somatostatin-receptor-2-in-the-context-of-antidepressant-treatment-response-in-chronic-mild-stress-in-rat
#8
Agata Faron-Górecka, Maciej Kuśmider, Joanna Solich, Magdalena Kolasa, Paulina Pabian, Piotr Gruca, Irena Romańska, Dariusz Żurawek, Marta Szlachta, Mariusz Papp, Lucyna Antkiewicz-Michaluk, Marta Dziedzicka-Wasylewska
RATIONALE: The role of somatostatin and its receptors for the stress-related neuropsychiatric disorders has been widely raised. Recently, we have also demonstrated the involvement of somatostatin receptor type 2-sst2R and dopamine receptor type 2-D2R in stress. OBJECTIVE: In this context, we decided to find if these receptors are involved in response to antidepressant treatment in animal model of depression-chronic mild stress (CMS). METHODS: Here, we report data obtained following 7-week CMS procedure...
April 30, 2018: Psychopharmacology
https://www.readbyqxmd.com/read/29705915/molecular-mapping-and-inheritance-of-restoration-of-fertility-rf-in-a4-hybrid-system-in-pigeonpea-cajanus-cajan-l-millsp
#9
Rachit K Saxena, Kishan Patel, C V Sameer Kumar, Kuldeep Tyagi, K B Saxena, Rajeev K Varshney
We report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer lines. Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and identify quantitative trait loci (QTL) associated with restoration of fertility (Rf)...
April 28, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29700394/stage-specific-effects-of-bioactive-lipids-on-human-ipsc-cardiac-differentiation-and-cardiomyocyte-proliferation
#10
Arun Sharma, Yuan Zhang, Jan W Buikema, Vahid Serpooshan, Orlando Chirikian, Nina Kosaric, Jared M Churko, Elda Dzilic, Alice Shieh, Paul W Burridge, Joseph C Wu, Sean M Wu
Bioactive lipids such as sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) regulate diverse processes including cell proliferation, differentiation, and migration. However, their roles in cardiac differentiation and cardiomyocyte proliferation have not been explored. Using a 96-well differentiation platform for generating human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) we found that S1P and LPA can independently enhance cardiomyocyte generation when administered at an early stage of differentiation...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29698509/microrna-425-and-microrna-155-cooperatively-regulate-atrial-natriuretic-peptide-expression-and-cgmp-production
#11
Sara Vandenwijngaert, Clara D Ledsky, Obiajulu Agha, Connie Wu, Dongjian Hu, Aranya Bagchi, Ibrahim J Domian, Emmanuel S Buys, Christopher Newton-Cheh, Donald B Bloch
AIMS: Atrial natriuretic peptide (ANP), secreted primarily by atrial cardiomyocytes, decreases blood pressure by raising cyclic 3',5'-guanosine monophosphate (cGMP) levels and inducing vasorelaxation, natriuresis, and diuresis. Raising the level of ANP has been shown to be an effective treatment for hypertension. To advance the future development of an anti-microRNA (miR) approach to increasing expression of ANP, we investigated the regulation of NPPA expression by two miRs: miR-425 and miR-155...
2018: PloS One
https://www.readbyqxmd.com/read/29693249/yes-associated-protein-mediates-human-embryonic-stem-cell-derived-cardiomyocyte-proliferation-involvement-of-epidermal-growth-factor-receptor-signaling
#12
Somi Park, Museog Choe, Hancheol Yeo, Hojae Han, Joongsun Kim, Woocheol Chang, Seungpil Yun, Hojin Lee, Minyoung Lee
Unlike mature cardiomyocytes, human pluripotent stem cell-derived cardiomyocytes exhibit higher proliferative capacity; however, the underlying mechanisms involved are yet to be elucidated. Here, we revealed that the Yes-associated protein (YAP) plays a critical role in regulating cell proliferation in association with epidermal growth factor receptor (EGFR) in human embryonic stem cell-derived cardiomyocytes (hESC-CMs). Our results show that low-density culture significantly promotes the proliferation of hESC-CMs via YAP...
April 25, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29689549/cardiomyocyte-derived-mir-328-promotes-cardiac-fibrosis-by-paracrinely-regulating-adjacent-fibroblasts
#13
Dandan Zhao, Cui Li, He Yan, Tianyu Li, Ming Qian, Nan Zheng, Hua Jiang, Li Liu, Bozhi Xu, Qiuxia Wu, Xuelian Li, Haihai Liang, Hongli Shan
BACKGROUND/AIMS: In our previous study, we demonstrated that elevated expression of miR-328 is a potent determinant of cardiac fibrosis during myocardial infarction (MI). In the present study, histological examination revealed progressive fibrosis in transgenic mice overexpressing cardiomyocyte-specific miR-328. This study investigated whether the transfer of miR-328 from cardiomyocytes (CMs) to cardiac fibroblasts (CFs) in a paracrine manner contributes to myocardial fibrosis. METHODS: Myocardial infarction was established by the occlusion of the left coronary artery...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29684447/type-3-iodothyronine-deiodinase-is-expressed-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#14
Kumiko Nishimura, Masafumi Takeda, Jun K Yamashita, Ichiro Shiojima, Nagaoki Toyoda
AIMS: Type 3 iodothyronine deiodinase (D3), which converts thyroxine (T4 ) and 3,5,3'-triiodothyronine (T3 ) to 3,3',5'-triiodothyronine (rT3 ) and 3,3'-diiodothyronine (T2 ), respectively, inactivates thyroid hormones. We investigated the expression and regulation of D3 in human cardiomyocytes which were differentiated from human induced pluripotent stem cells (hiPSCs). MAIN METHODS: We characterized D3 activity using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis...
April 20, 2018: Life Sciences
https://www.readbyqxmd.com/read/29667000/a-novel-mitochondrial-orf147-causes-cytoplasmic-male-sterility-in-pigeonpea-by-modulating-aberrant-anther-dehiscence
#15
Pooja Bhatnagar-Mathur, Ranadheer Gupta, Palakolanu Sudhakar Reddy, Bommineni Pradeep Reddy, Dumbala Srinivas Reddy, C V Sameerkumar, Rachit Kumar Saxena, Kiran K Sharma
A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytoplasmic male sterility (CMS). We identified a novel ORF (orf147) from the A4 cytoplasm of C. cajanifolius that was created via rearrangements in the CMS line and co-transcribes with the known and unknown sequences...
April 17, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29665845/deletion-of-hp1%C3%AE-in-cardiac-myocytes-affects-h4k20me3-levels-but-does-not-impact-cardiac-growth
#16
Kyohei Oyama, Danny El-Nachef, Chen Fang, Hidemi Kajimoto, Jeremy P Brown, Prim B Singh, W Robb MacLellan
BACKGROUND: Heterochromatin, which is formed when tri-methyl lysine 9 of histone H3 (H3K9me3) is bound by heterochromatin 1 proteins (HP1s), plays an important role in differentiation and senescence by silencing cell cycle genes. Cardiac myocytes (CMs) accumulate heterochromatin during differentiation and demethylation of H3K9me3 inhibits cell cycle gene silencing and cell cycle exit in CMs; however, it is unclear if this process is mediated by HP1s. In this study, we created a conditional CM-specific HP1 gamma (HP1γ) knockout (KO) mouse model and tested whether HP1γ is required for cell cycle gene silencing and cardiac growth...
April 17, 2018: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29661985/a-net-shaped-multicellular-formation-facilitates-the-maturation-of-hpsc-derived-cardiomyocytes-through-mechanical-and-electrophysiological-stimuli
#17
Taoyan Liu, Chengwu Huang, Hongxia Li, Fujian Wu, Jianwen Luo, Wenjing Lu, Feng Lan
The use of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is limited in drug discovery and cardiac disease mechanism studies due to cell immaturity. Although many approaches have been reported to improve the maturation of hiPSC-CMs, the elucidation of the process of maturation is crucial. We applied a small-molecule-based differentiation method to generate cardiomyocytes (CMs) with multiple aggregation forms. The motion analysis revealed significant physical differences in the differently shaped CMs, and the net-shaped CMs had larger motion amplitudes and faster velocities than the sheet-shaped CMs...
April 14, 2018: Aging
https://www.readbyqxmd.com/read/29644438/comparative-phosphoproteomic-analysis-reveals-differentially-phosphorylated-proteins-regulate-anther-and-pollen-development-in-kenaf-cytoplasmic-male-sterility-line
#18
Peng Chen, Ru Li, Ruiyang Zhou
Cytoplasmic male sterility (CMS) is widely used in plant breeding and represents a perfect model to understand cyto-nuclear interactions and pollen development research. Protein phosphorylation is ubiquitous and is involved in the regulation of diverse cellular processes. To reveal the possible mechanism of CMS and pollen development in kenaf, we performed an iTRAQ-based comparative phosphoproteome analysis in the anthers of a CMS line and wild-type plant (Wt). Whole transcriptome unigenes of kenaf as the reference genome, we identified a total of 3045 phosphorylated sites on 1640 peptides corresponding to 974 unique proteins...
April 11, 2018: Amino Acids
https://www.readbyqxmd.com/read/29643704/bioinformatic-analysis-of-the-possible-regulative-network-of-mir-30a-e-in-cardiomyocytes-2-days-post-myocardial-infarction
#19
Yiping Wang, Yingqiong Huang, Min Zhang, Xiaoqin Zhang, Xuemei Tang, Yan Kang
Background: Both miR-30a and miR-30e are significantly downregulated in cardiomyocytes (CMs) 2 days (d) post myocardial infarction (MI). This study aimed to identify their possible regulative network in CMs 2d post-MI. Methods: The dysregulated mRNAs in left ventricle tissues 2d post-MI in mice model were retrieved from one previous publication. The verified target genes of miR-30a/e and the predicted targets (upregulated 2d post-MI) were subjected to analysis of the involvement in biological processes according to their enrichment in gene ontology (GO) terms...
March 2018: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/29626311/exploration-of-mirnas-and-target-genes-of-cytoplasmic-male-sterility-line-in-cotton-during-flower-bud-development
#20
Hushuai Nie, Yumei Wang, Ying Su, Jinping Hua
Cytoplasmic male sterility (CMS) lines provide crucial material to harness heterosis for crop plants, which serves as an important strategy for hybrid seed production. However, the molecular mechanism remains obscure. Although microRNAs (miRNAs) play important roles in vegetative growth and reproductive growth, there are few reports on miRNAs regulating the development of male sterility in Upland cotton. In present study, 12 small RNA libraries were constructed and sequenced for two development stages of flower buds from a CMS line and its maintainer line...
April 7, 2018: Functional & Integrative Genomics
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