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https://www.readbyqxmd.com/read/28528003/acinetobacter-baumannii-k11-and-k83-capsular-polysaccharides-have-the-same-6-deoxy-l-talose-containing-pentasaccharide-k-units-but-different-linkages-between-the-k-units
#1
Johanna J Kenyon, Alexander S Shashkov, Sof'ya N Senchenkova, Mikhail M Shneider, Bin Liu, Anastasiya V Popova, Nikolay P Arbatsky, Konstantin A Miroshnikov, Lei Wang, Yuriy A Knirel, Ruth M Hall
Acinetobacter baumannii produces a variety of capsular polysaccharides (CPS) via genes located at the chromosomal K locus and some KL gene clusters include genes for the synthesis of specific sugars. The structures of K11 and K83 CPS produced by isolates LUH5545 and LUH5538, which carry related KL11a and KL83 gene clusters, respectively, were established by sugar analysis and one- and two-dimensional (1)H and (13)C NMR spectroscopy. Both CPS contain L-rhamnose (L-Rha) and 6-deoxy-L-talose (L-6dTal), and both KL gene clusters include genes for dTDP-L-Rhap synthesis and a tle (talose epimerase) gene encoding an epimerase that converts dTDP-L-Rhap to dTDP-L-6dTalp...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527793/prevalence-of-non-aureus-staphylococcus-species-causing-intramammary-infections-in-canadian-dairy-herds
#2
Larissa A Z Condas, Jeroen De Buck, Diego B Nobrega, Domonique A Carson, Sohail Naushad, Sarne De Vliegher, Ruth N Zadoks, John R Middleton, Simon Dufour, John P Kastelic, Herman W Barkema
Non-aureus staphylococci (NAS), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. To establish their importance as a group, the distribution of individual species needs to be determined. In the present study, NAS intramammary infection (IMI) was defined as a milk sample containing ≥1,000 cfu/mL in pure or mixed culture that was obtained from a cohort of cows assembled by the Canadian Bovine Mastitis Research Network. Overall, 6,213 (6.3%) of 98,233 quarter-milk samples from 5,149 cows and 20,305 udder quarters were associated with an NAS IMI...
May 17, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28527256/-stat3-activation-by-hypoxia-in-in-vitro-models-of-cervix-cancer-and-endothelial-cells
#3
Óscar Ortega, Alejandro Ondo-Méndez, Ruth Garzón
INTRODUCTION: The biological behavior of cancer cells is influenced by the tumor microenvironment in which they develop. In this context, stressor stimuli such as hypoxia are considered critical for tumor development and therapeutic management. Cellular response to various stimuli is evidenced in the activation of intracellular signaling pathways such as JAK/STAT, which is one of the most important for its effects in differentiation and cell proliferation. OBJECTIVE: To evaluate the condition of the JAK/STAT pathway through the expression/activation of the STAT3 protein in cervix cancer cells (HeLa) and endothelial cells (EA...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28526819/systematic-comparison-of-2a-peptides-for-cloning-multi-genes-in-a-polycistronic-vector
#4
Ziqing Liu, Olivia Chen, J Blake Joseph Wall, Michael Zheng, Yang Zhou, Li Wang, Haley Ruth Vaseghi, Li Qian, Jiandong Liu
Cloning of multiple genes in a single vector has greatly facilitated both basic and translational studies that require co-expression of multiple factors or multi-units of complex protein. Many strategies have been adopted, among which 2A "self-cleaving" peptides have garnered increased interest for their polycistronic nature, small size and high "cleavage" efficiency. However, broad application of 2 A peptides is limited by the lack of systematic comparison of different 2As alone or in combination. Here we characterized the effect of varying gene position and 2As on the expression of proteins encoded in bi-, tri-, or quad-cistronic constructs...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526673/the-effectiveness-of-a-computer-game-based-rehabilitation-platform-for-children-with-cerebral-palsy-protocol-for-a-randomized-clinical-trial
#5
Anuprita Kanitkar, Tony Szturm, Sanjay Parmar, Dorcas Bc Gandhi, Gina Ruth Rempel, Gayle Restall, Monika Sharma, Amitesh Narayan, Jeyaraj Pandian, Nilashri Naik, Ravi R Savadatti, Mahesh Appasaheb Kamate
BACKGROUND: It is difficult to engage young children with cerebral palsy (CP) in repetitive, tedious therapy. As such, there is a need for innovative approaches and tools to motivate these children. We developed the low-cost, computer game-based rehabilitation platform CGR that combines fine manipulation and gross movement exercises with attention and planning game activities appropriate for young children with CP. OBJECTIVE: The objective of this study is to provide evidence of the therapeutic value of CGR to improve upper extremity (UE) motor function for children with CP...
May 18, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28526481/randomised-control-trial-of-the-impact-of-a-brief-tele-support-intervention-on-initial-attendance-at-physiotherapy-group-sessions-for-pelvic-floor-problems
#6
Lisa A Osborne, C Mair Whittall, Ruth Emanuel, Simon Emery, Phil Reed
OBJECTIVE: To assesses the efficacy of a brief telephone call to patients on a waiting list for physiotherapy treatment for pelvic floor dysfunction on initial attendance. DESIGN: A three-armed randomized control study. SETTING: An outpatient physiotherapy clinic at a hospital. PARTICIPANTS: 130 consecutively referred women patients (mean age of 51.47, range = 26-84) with a variety of pelvic floor dysfunction problems...
May 16, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28526357/universal-motorcycle-helmet-laws-to-reduce-injuries-a-community-guide-systematic-review
#7
REVIEW
Yinan Peng, Namita Vaidya, Ramona Finnie, Jeffrey Reynolds, Cristian Dumitru, Gibril Njie, Randy Elder, Rebecca Ivers, Chika Sakashita, Ruth A Shults, David A Sleet, Richard P Compton
CONTEXT: Motorcycle crashes account for a disproportionate number of motor vehicle deaths and injuries in the U.S. Motorcycle helmet use can lead to an estimated 42% reduction in risk for fatal injuries and a 69% reduction in risk for head injuries. However, helmet use in the U.S. has been declining and was at 60% in 2013. The current review examines the effectiveness of motorcycle helmet laws in increasing helmet use and reducing motorcycle-related deaths and injuries. EVIDENCE ACQUISITION: Databases relevant to health or transportation were searched from database inception to August 2012...
June 2017: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28526039/influences-on-patient-satisfaction-in-healthcare-centers-a-semi-quantitative-study-over-5%C3%A2-years
#8
Ruth D Thornton, Nicole Nurse, Laura Snavely, Stacey Hackett-Zahler, Kenice Frank, Robert A DiTomasso
BACKGROUND: Knowledge of ambulatory patients' satisfaction with clinic visits help improve communication and delivery of healthcare. The goal was to examine patient satisfaction in a primary care setting, identify how selected patient and physician setting and characteristics affected satisfaction, and determine if feedback provided to medical directors over time impacted patient satisfaction. METHODS: A three-phase, semi-quantitative analysis was performed using anonymous, validated patient satisfaction surveys collected from 889 ambulatory outpatients in 6 healthcare centers over 5-years...
May 19, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28525932/endoscopic-ultrasound-of-duodenal-heterotopic-gastric-mucosa
#9
Malay Sharma, Piyush Somani, Krishnaveni Janarthanan, Saurabh Jindal, Rajendra Prasad, Ruth Shifa Hari
No abstract text is available yet for this article.
May 19, 2017: Endoscopy
https://www.readbyqxmd.com/read/28525921/a-new-electrogram-based-diagnostic-algorithm-to-improve-the-left-ventricular-effective-pacing-detection-corrected-a-non-response-to-cardiac-resynchronization-therapy-pacing
#10
Roberto Matía, Antonio Hernández-Madrid, Ruth Nicholson Klepfer, Subham Ghosh, Gonzalo Sánchez-Huete, Javier Moreno
No abstract text is available yet for this article.
May 1, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28525597/influenza-vaccination-modifies-disease-severity-among-community-dwelling-adults-hospitalized-with-influenza
#11
Carmen Sofia Arriola, Shikha Garg, Evan J Anderson, Patricia A Ryan, Andrea George, Shelley M Zansky, Nancy Bennett, Arthur Reingold, Marisa Bargsten, Lisa Miller, Kimberly Yousey-Hindes, Lilian Tatham, Susan R Bohm, Ruth Lynfield, Ann Thomas, Mary Lou Lindegren, William Schaffner, Alicia M Fry, Sandra S Chaves
Background: We investigated the effect of influenza vaccination on disease severity in adults hospitalized with laboratory-confirmed influenza during 2013-14, a season in which vaccine viruses were antigenically similar to those circulating. Methods: We analyzed data from the 2013-14 influenza season, and used propensity score matching to account for the probability of vaccination within age strata (18-49, 50-64 and ≥65 years). Death, intensive care unit (ICU) admission, and hospital and ICU lengths of stay (LOS) were outcome measures for severity...
May 19, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28524746/a-contemporary-profile-of-primary-progressive-multiple-sclerosis-participants-from-the-narcoms-registry
#12
Amber Salter, Nina P Thomas, Tuula Tyry, Gary R Cutter, Ruth Ann Marrie
BACKGROUND: Primary progressive multiple sclerosis (PPMS) represents 10%-15% of all multiple sclerosis (MS) diagnoses. Information regarding socio-demographic and clinical characteristics of persons with PPMS is limited. OBJECTIVE: To characterize persons with PPMS in the North American Research Committee on Multiple Sclerosis (NARCOMS) Registry. METHODS: We compared demographic and health-related characteristics of NARCOMS Registry participants reporting PPMS in the spring 2015 update survey with those reporting relapsing-remitting multiple sclerosis (RRMS) and secondary progressive multiple sclerosis (SPMS), with characteristics of published PPMS cohorts...
May 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28524735/predictors-of-gestational-weight-gain-among-chilean-pregnant-women-the-chilean-maternal-and-infant-nutrition-cohort-study
#13
Maria Luisa Garmendia, Susana Mondschein, Omar Matus, Ruth Murrugarra, Ricardo Uauy
We identified factors associated with gestational weight gain (GWG) in 1,654 Chilean pregnant women with full-term pregnancies. At baseline, we collected information about sociodemographic, gyneco-obstetric, anthropometric, and health care-related factors. We found that pre-pregnancy nutritional body mass index (BMI) was the most important factor related to GWG above recommendations (overweight: RRR = 2.31, 95% CI (1.73, 3.09) and obesity: RRR = 2.90, 95% CI (2.08, 4.03)). We believe that women who are overweight/obese at the beginning of pregnancy should be identified because of their higher risk and that adequate strategies should be designed and implemented to help them achieve a healthy GWG...
May 19, 2017: Health Care for Women International
https://www.readbyqxmd.com/read/28523881/bumps-in-the-road-panniculitis-in-children-and-adolescents-treated-with-vemurafenib
#14
Nika Finelt, Rishi R Lulla, Hector Melin-Aldana, Jennifer Shuley Ruth, Frank Y Lin, Jack M Su, Crystal Y Pourciau, Raegan D Hunt, Brandi M Kenner-Bell
Vemurafenib is increasingly being used to treat nonmelanoma tumors that are positive for the BRAF V600E mutation. We report three children who presented with panniculitis induced by vemurafenib while undergoing treatment for central nervous system tumors and review the literature.
May 2017: Pediatric Dermatology
https://www.readbyqxmd.com/read/28523456/bone-marrow-mesenchymal-stem-cell-administration-significantly-improves-outcome-after-retinal-ischemia-in-rats
#15
Biji Mathew, Jacqueline N Poston, John C Dreixler, Leianne Torres, Jasmine Lopez, Ruth Zelkha, Irina Balyasnikova, Maciej S Lesniak, Steven Roth
PURPOSE: Ischemia-associated retinal degeneration is one of the leading causes of vision loss, and to date, there are no effective treatment options. We hypothesized that delayed injection of bone-marrow stem cells (BMSCs) 24 h after the onset of ischemia could effectively rescue ischemic retina from its consequences, including apoptosis, inflammation, and increased vascular permeability, thereby preventing retinal cell loss. METHODS: Retinal ischemia was induced in adult Wistar rats by increasing intraocular pressure (IOP) to 130-135 mmHg for 55 min...
May 18, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28523217/relation-between-patterns-of-intrinsic-network-connectivity-cognitive-functioning-and-symptom-presentation-in-trauma-exposed-patients-with-major-depressive-disorder
#16
Melissa Parlar, Maria Densmore, Geoffrey B Hall, Paul A Frewen, Ruth A Lanius, Margaret C McKinnon
OBJECTIVE: The present study investigated resting fMRI connectivity within the default mode (DMN), salience (SN), and central executive (CEN) networks in relation to neurocognitive performance and symptom severity in trauma-exposed patients with major depressive disorder (MDD). METHOD: Group independent component analysis was conducted among patients with MDD (n = 21), examining DMN, SN, and CEN connectivity in relation to neurocognitive performance and symptom severity...
May 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28522448/estimation-of-the-number-of-women-living-with-metastatic-breast-cancer-in-the-united-states
#17
Angela B Mariotto, Ruth Etzioni, Marc Hurlbert, Lynne Penberthy, Musa Mayer
Background: Distant metastatic breast cancer (MBC), including metastases found at diagnosis (de novo) and those occurring later (recurrence), represents the most severe form of the disease, when resource utilization is most intensive. Yet, the number of women living with MBC in the United States is unknown. The objective of this article is to use population-based data to estimate the prevalence of MBC.Methods: We used a back-calculation method to estimate MBC prevalence from U.S. breast cancer mortality and survival from the Surveillance, Epidemiology and End Results (SEER) registries...
May 18, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28522001/cytotoxic-effect-of-a-mannogalactoglucan-extracted-from-agaricus-bisporus-on-hepg2-cells
#18
Amanda do Rocio Andrade Pires, Andrea Caroline Ruthes, Silvia Maria Suter Correia Cadena, Marcello Iacomini
A mannogalactoglucan (RK2-Ab; Mw 1.8×10(4)gmol(-1)) composed by Man (27.3%), Gal (24.4%) and Glc (48.3%) was extracted and characterized from Agaricus bisporus, and its biological activity was evaluated on human hepatocarcinoma cells (HepG2). The partially-O-methylated alditol acetates together with the NMR data suggest the main chain to be composed of α-d-Galp (32.8%) and β-d-Glcp (37.0%) units (1→6)-linked, with β-d-Manp (14.6%), as non-reducing end units, substituting the side chains at O-2 (α-d-Galp units; 3...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28520984/genome-wide-association-study-of-heavy-smoking-and-daily-nondaily-smoking-in-the-hispanic-community-health-study-study-of-latinos-hchs-sol
#19
Nancy L Saccone, Leslie S Emery, Tamar Sofer, Stephanie M Gogarten, Diane M Becker, Erwin P Bottinger, Li-Shiun Chen, Robert C Culverhouse, Weimin Duan, Dana B Hancock, H Dean Hosgood, Eric O Johnson, Ruth J F Loos, Tin Louie, George Papanicolaou, Krista M Perreira, Erik J Rodriquez, Claudia Schurmann, Adrienne M Stilp, Adam A Szpiro, Gregory A Talavera, Kent D Taylor, James F Thrasher, Lisa R Yanek, Cathy C Laurie, Eliseo J Pérez-Stable, Laura J Bierut, Robert C Kaplan
Introduction: Genetic variants associated with nicotine dependence have previously been identified, primarily in European-ancestry populations. No genome-wide association studies (GWAS) have been reported for smoking behaviors in Hispanics/Latinos in the U.S. and Latin America, who are of mixed ancestry with European, African, and American Indigenous components. Methods: We examined genetic associations with smoking behaviors in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) [N=12,741 with smoking data, 5,119 ever smokers], using ~2...
May 17, 2017: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/28520482/foodborne-disease-outbreaks-in-correctional-institutions-united-states-1998-2014
#20
Mariel A Marlow, Ruth E Luna-Gierke, Patricia M Griffin, Antonio R Vieira
OBJECTIVES: To present the first update on the epidemiology of US foodborne correctional institution outbreaks in 20 years. METHODS: We analyzed data from the Centers for Disease Control and Prevention's Foodborne Disease Outbreak Surveillance System to describe correctional institution outbreaks from 1998 to 2014 and compare them with other foodborne outbreaks. RESULTS: Two hundred foodborne outbreaks in correctional institutions were reported, resulting in 20 625 illnesses, 204 hospitalizations, and 5 deaths...
May 18, 2017: American Journal of Public Health
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