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https://www.readbyqxmd.com/read/29350722/chemical-decontamination-of-ips-cell-derived-neural-cell-mixtures
#1
Di Mao, Xie Khim Watson Chung, Tomoko Andoh-Noda, Ying Qin, Shin-Ichi Sato, Yasushi Takemoto, Wado Akamatsu, Hideyuki Okano, Motonari Uesugi
This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.
January 19, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29345014/human-induced-pluripotent-stem-cell-models-of-retinitis-pigmentosa
#2
REVIEW
Ana Artero Castro, Dunja Lukovic, Pavla Jendelova, Slaven Erceg
Hereditary retinal dystrophies, specifically retinitis pigmentosa (RP) are clinically and genetically heterogeneous diseases affecting primarily retinal cells and retinal pigment epithelial (RPE) cells with blindness as a final outcome. Understanding the pathogenicity behind these diseases has been largely precluded by the unavailability of affected tissue from patients, large genetic heterogeneity and animal models that do not faithfully represent some human diseases. A landmark discovery of human induced pluripotent stem cells (hiPSC) permitted the derivation of patient-specific cells...
January 18, 2018: Stem Cells
https://www.readbyqxmd.com/read/29339826/using-intracellular-markers-to-identify-a-novel-set-of-surface-markers-for-live-cell-purification-from-a-heterogeneous-hipsc-culture
#3
Elizabeth J Paik, Alison L O'Neil, Shi-Yan Ng, Chicheng Sun, Lee L Rubin
Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can provide sources for midbrain dopaminergic (mDA) neural progenitors (NPCs) for cell therapy to treat Parkinson's disease (PD) patients. However, the well-known line-to-cell line variability in the differentiation capacity of individual cell lines needs to be improved for the success of this therapy. To address this issue, we sought to identify mDA NPC specific cell surface markers for fluorescence activated cell sorting (FACS). Through RNA isolation after sorting for NPCs based on staining for cell-specific transcription factors followed by microarray, we identified two positive cell surface markers (CORIN and CD166) and one negative cell surface marker (CXCR4) for mDA NPC sorting...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29337120/deriving-dorsal-spinal-sensory-interneurons-from-human-pluripotent-stem%C3%A2-cells
#4
Sandeep Gupta, Daniel Sivalingam, Samantha Hain, Christian Makkar, Enrique Sosa, Amander Clark, Samantha J Butler
Cellular replacement therapies for neurological conditions use human embryonic stem cell (hESC)- or induced pluripotent stem cell (hiPSC)-derived neurons to replace damaged or diseased populations of neurons. For the spinal cord, significant progress has been made generating the in-vitro-derived motor neurons required to restore coordinated movement. However, there is as yet no protocol to generate in-vitro-derived sensory interneurons (INs), which permit perception of the environment. Here, we report on the development of a directed differentiation protocol to derive sensory INs for both hESCs and hiPSCs...
January 9, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29337067/the-impact-of-growth-factors-on-human-induced-pluripotent-stem-cells-differentiation-into-cardiomyocytes
#5
Marianne E Yassa, Iman A Mansour, Nadia I Sewelam, Hala Hamza, Taghrid Gaafar
Human induced pluripotent stem cells (hiPSCs) act as a promising therapeutic alternative for cardiovascular diseases. They yield a large number of functional cardiomyocytes (CMs) from autologous cell sources without ethical or immunological problems. However, significant limitations still remain in terms of line-to-line variability in CM yield and reproducibility. AIM: To efficiently enhance NP0040 hiPSCs differentiation into CMs. MAIN METHODS: Following a standard cardiac differentiation protocol using small molecules targeting the canonical Wnt signaling, growth factors (BMP4 and FGF2) and ascorbic acid were added further in order to increase the cardiac differentiation efficiency...
January 11, 2018: Life Sciences
https://www.readbyqxmd.com/read/29334628/production-and-characterization-of-human-induced-pluripotent-stem-cells-ipscs-from-joubert-syndrome-cssi001-a-2850
#6
Jessica Rosati, Filomena Altieri, Silvia Tardivo, Elisa Maria Turco, Marina Goldoni, Iolanda Spasari, Daniela Ferrari, Laura Bernardini, Giuseppe Lamorte, Enza Maria Valente, Angelo Luigi Vescovi
Joubert Syndrome (JS) is a rare autosomal recessive or X-linked condition characterized by a peculiar cerebellar malformation, known as the molar tooth sign (MTS), associated with other neurological phenotypes and multiorgan involvement. JS is a ciliopathy, a spectrum of disorders whose causative genes encode proteins involved in the primary cilium apparatus. In order to elucidate ciliopathy-associated molecular mechanisms, human induced pluripotent stem cells (hiPSCs) were derived from a patient affected by JS carrying a homozygous missense mutation in the AHI1 gene (p...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29330131/response-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-to-several-pharmacological-agents-when-intrinsic-syncytial-pacing-is-overcome-by-acute-external-stimulation
#7
Haoyu Zeng, Bharathi Balasubramanian, Armando Lagrutta, Frederick Sannajust
We challenged human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) syncytia, mainly, CDI iCells with several classes of well-characterized pharmacological agents (including hERG blocker, Nav1.5 blocker, Cav1.2 blocker and opener, β-adrenergic agonist, and If blocker) under pacing conditions, utilizing the Cardio-ECR instrument, a non-invasive platform featuring simultaneous and continuous measurement of synchronized beating rate and contractility (both signals were acquired simultaneously and well aligned) ...
January 9, 2018: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/29323137/issues-with-the-specificity-of-immunological-reagents-for-nlrp3-implications-for-age-related-macular-degeneration
#8
Cassandra Kosmidou, Nikolaos E Efstathiou, Mien V Hoang, Shoji Notomi, Eleni K Konstantinou, Masayuki Hirano, Kosuke Takahashi, Daniel E Maidana, Pavlina Tsoka, Lucy Young, Evangelos S Gragoudas, Timothy W Olsen, Yuki Morizane, Joan W Miller, Demetrios G Vavvas
Contradictory data have been presented regarding the implication of the NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome in age-related macular degeneration (AMD), the leading cause of vision loss in the Western world. Recognizing that antibody specificity may explain this discrepancy and in line with recent National Institutes of Health (NIH) guidelines requiring authentication of key biological resources, the specificity of anti-NLRP3 antibodies was assessed to elucidate whether non-immune RPE cells express NLRP3...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321049/human-liver-organoids-generated-with-single-donor-derived-multiple-cells-rescue-mice-from-acute-liver-failure
#9
Yun-Zhong Nie, Yun-Wen Zheng, Miyuki Ogawa, Etsuko Miyagi, Hideki Taniguchi
BACKGROUND: Acute liver failure (ALF) is a life-threatening disease with a high mortality rate. However, there are limited treatments or devices available for ALF therapy. Here, we aimed to develop a new strategy for ALF treatment by transplanting functional liver organoids (LOs) generated from single donor-derived human induced pluripotent stem cell (hiPSC) endoderm, endothelial cells (ECs), and mesenchymal cells (MCs). METHODS: First, we isolated ECs and MCs from a single donor umbilical cord (UC) through enzyme digestion and characterized the UC-ECs and UC-MCs by flow cytometry...
January 10, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29320747/identification-of-cardiomyocyte-fated-progenitors-from-human-induced-pluripotent-stem-cells-marked-with-cd82
#10
Masafumi Takeda, Yasuharu Kanki, Hidetoshi Masumoto, Shunsuke Funakoshi, Takeshi Hatani, Hiroyuki Fukushima, Akashi Izumi-Taguchi, Yusuke Matsui, Teppei Shimamura, Yoshinori Yoshida, Jun K Yamashita
Here, we find that human-induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM)-fated progenitors (CFPs) that express a tetraspanin family glycoprotein, CD82, almost exclusively differentiate into CMs both in vitro and in vivo. CD82 is transiently expressed in late-stage mesoderm cells during hiPSC differentiation. Purified CD82+ cells gave rise to CMs under nonspecific in vitro culture conditions with serum, as well as in vivo after transplantation to the subrenal space or injured hearts in mice, indicating that CD82 successfully marks CFPs...
January 9, 2018: Cell Reports
https://www.readbyqxmd.com/read/29315416/human-induced-pluripotent-stem-cells-and-male-infertility-an-overview-of-current-progress-and-perspectives
#11
Fang Fang, Zili Li, Qian Zhao, Honggang Li, Chengliang Xiong
Recently, significant progress has been made in ART for the treatment of male infertility. However, current ART has failed to help infertile patients with non-obstructive azoospermia, unless donor sperm is used. In fact, most couples wish to have their own genetically related child. Human induced pluripotent stem cells (hiPSCs) can be generated from patients' somatic cells and in vitro derivation of functional germ cells from patient-specific iPSCs may provide new therapeutic strategies for infertile couples...
January 5, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29312819/small-scale-screening-of-anticancer-drugs-acting-specifically-on-neural-stem-progenitor-cells-derived-from-human-induced-pluripotent-stem-cells-using-a-time-course-cytotoxicity-test
#12
Hayato Fukusumi, Yukako Handa, Tomoko Shofuda, Yonehiro Kanemura
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central nervous system. However, owing to their intrinsic proliferative potential, therapies using transplanted hiPSC-NSPCs carry an inherent risk of undesired growth in vivo. Therefore, it is important to find cytotoxic drugs that can specifically target overproliferative transplanted hiPSC-NSPCs without damaging the intrinsic in vivo stem-cell system...
2018: PeerJ
https://www.readbyqxmd.com/read/29305588/short-term-retinoic-acid-treatment-sustains-pluripotency-and-suppresses-differentiation-of-human-induced-pluripotent-stem-cells
#13
Maria Teresa De Angelis, Elvira Immacolata Parrotta, Gianluca Santamaria, Giovanni Cuda
Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) derived from blastocyst and human induced pluripotent stem cells (hiPSCs) generated from somatic cells by ectopic expression of defined transcriptional factors, have both the ability to self-renew and to differentiate into all cell types. Here we explored the two antagonistic effects of retinoic acid (RA) on hiPSCs. Although RA has been widely described as a pharmacological agent with a critical role in initiating differentiation of pluripotent stem cells, we demonstrate that short-term RA exposure not only antagonizes cell differentiation and sustains pluripotency of hiPSCs, but it also boosts and improves their properties and characteristics...
January 5, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29298685/time-course-transcriptome-analysis-of-human-cellular-reprogramming-from-multiple-cell-types-reveals-the-drastic-change-occurs-between-the-mid-phase-and-the-late-phase
#14
Akihiro Kuno, Ken Nishimura, Satoru Takahashi
BACKGROUND: Human induced pluripotent stem cells (hiPSCs) have been attempted for clinical application with diverse iPSCs sources derived from various cell types. This proposes that there would be a shared reprogramming route regardless of different starting cell types. However, the insights of reprogramming process are mostly restricted to only fibroblasts of both human and mouse. To understand molecular mechanisms of cellular reprogramming, the investigation of the conserved reprogramming routes from various cell types is needed...
January 3, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29290803/glutathione-peroxidase-3-delivered-by-hipsc-mscs-ameliorated-hepatic-ir-injury-via-inhibition-of-hepatic-senescence
#15
Xiang Qi, Kevin Tak-Pan Ng, Qizhou Lian, Chang Xian Li, Wei Geng, Chang Chun Ling, Wai Ho Yeung, Yuen Yuen Ma, Xiao Bing Liu, Hui Liu, Jiang Liu, Xin Xiang Yang, Chung Mau Lo, Kwan Man
Background and Aims: Down-regulation of GPx3 accelerated hepatic senescence, which further caused overwhelming inflammation and severe liver graft injury. MSCs derived from human induced pluripotent stem cells (hiPSC-MSCs) have been developed as more efficient delivery vehicle with the property of injury tropism. Here, we aimed to explore the suppressive role of GPx3 in hepatic IR injury using novel delivery system of hiPSC-MSCs. Methods: The mice IR injury model with partial hepatectomy was established. The engineered hiPSC-MSCs delivering GPx3 was constructed...
2018: Theranostics
https://www.readbyqxmd.com/read/29288713/the-effects-of-gentamicin-and-penicillin-streptomycin-on-the-electrophysiology-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-in-manual-patch-clamp-and-multi-electrode-array-system
#16
Soo-Wang Hyun, Bo-Ram Kim, Dan Lin, Sung-Ae Hyun, Seong Shoon Yoon, Joung-Wook Seo
INTRODUCTION: Cell culture media usually contains antibiotics including gentamicin or penicillin/streptomycin (PS) to protect cells from bacterial contamination. However, little is known about the effects of antibiotics on action potential and field potential parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). METHODS: The present study examined the effects of gentamicin (10, 25, and 50μg/ml) and PS (50, 100, and 200U/μg/ml) on electrophysiological activity in spontaneously beating hiPSC-CMs using manual patch clamp and multi-electrode array...
December 27, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/29278408/botulinum-hemagglutinin-mediated-in-situ-break-up-of-human-induced-pluripotent-stem-cell-aggregates-for-high-density-suspension-culture
#17
Suman Chandra Nath, Tokura Tomohiro, Mee-Hae Kim, Masahiro Kino-Oka
Large numbers of human induced pluripotent stem cells (hiPSCs) are required for making stable cell bank. Although suspension culture yields high cell numbers, there remain unresolved challenges for obtaining high-density of hiPSCs because large size aggregates exhibit low growth rates. Here, we established a simple method for hiPSC aggregate break-up using botulinum hemagglutinin (HA), which specifically bound with E-cadherin and disrupted cell-cell connections in hiPSC aggregates. HA showed temporary activity for disrupting the E-cadherin-mediated cell-cell connections to facilitate the break-up of aggregates into small sizes only 9 h after HA addition...
December 26, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29276154/modeling-the-pathogenesis-of-charcot-marie-tooth-disease-type-1a-using-patient-specific-ipscs
#18
Lei Shi, Lihua Huang, Ruojie He, Weijun Huang, Huiyan Wang, Xingqiang Lai, Zhengwei Zou, Jiaqi Sun, Qiong Ke, Minying Zheng, Xilin Lu, Zhong Pei, Huanxing Su, Andy Peng Xiang, Weiqiang Li, Xiaoli Yao
Charcot-Marie-Tooth disease type 1A (CMT1A), one of the most frequent inherited peripheral neuropathies, is associated with PMP22 gene duplication. Previous studies of CMT1A mainly relied on rodent models, and it is not yet clear how PMP22 overexpression leads to the phenotype in patients. Here, we generated the human induced pluripotent stem cell (hiPSC) lines from two CMT1A patients as an in vitro cell model. We found that, unlike the normal control cells, CMT1A hiPSCs rarely generated Schwann cells through neural crest stem cells (NCSCs)...
December 15, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29274391/electrophysiological-characteristics-and-pharmacological-sensitivity-of-two-lines-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-coming-from-two-different-suppliers
#19
Sonia Goineau, Vincent Castagné
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are increasingly used as preclinical tool for predicting drug-induced QT prolongation and arrhythmias. This study was conducted to assess the electrophysiological characteristics and the pharmacological sensitivity of two commercialized hiPSC-CMs. The baseline electrophysiological characteristics measured with a multi-electrode array (MEA) technology differ between Cor.4U and iCell2: higher beat rate (+32bpm) and shorter field potential duration (FPD, -201ms) for Cor...
December 20, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/29273803/mapping-regulatory-variants-in-hipsc-models
#20
Gabriel E Hoffman, Kristen J Brennand
No abstract text is available yet for this article.
January 2018: Nature Genetics
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