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ovulation trigger

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https://www.readbyqxmd.com/read/28427285/premenstrual-dysphoric-disorder-symptoms-following-ovarian-suppression-triggered-by-change-in-ovarian-steroid-levels-but-not-continuous-stable-levels
#1
Peter J Schmidt, Pedro E Martinez, Lynnette K Nieman, Deloris E Koziol, Karla D Thompson, Linda Schenkel, Paul G Wakim, David R Rubinow
OBJECTIVE: Premenstrual dysphoric disorder (PMDD) symptoms are eliminated by ovarian suppression and stimulated by administration of ovarian steroids, yet they appear with ovarian steroid levels indistinguishable from those in women without PMDD. Thus, symptoms could be precipitated either by an acute change in ovarian steroid levels or by stable levels above a critical threshold playing a permissive role in expression of an underlying infradian affective "pacemaker." The authors attempted to determine which condition triggers PMDD symptoms...
April 21, 2017: American Journal of Psychiatry
https://www.readbyqxmd.com/read/28426370/pin-formed-protein-a-door-to-reveal-the-mechanism-for-auxin-triggered-initiation-of-cotton-fiber
#2
Mi Zhang, Yuehua Xiao, Jianyan Zeng, Yan Pei
Cotton fibers are differentiated ovule epidermal cells that provide an ideal model to study cell differentiation and elongation. Establishment of auxin maximum in fiber cells is crucial for cotton-fiber protrusion from ovule surface. However, it is unclear where the auxin originates from and how the auxin accumulates in fiber cells. Our recent results indicate that the auxin is mainly imported from the outside of ovules, and transported to fiber cells through GhPIN (homolog of PIN-formed proteins in cotton) -mediated polar auxin transport, rather than in situ synthesis...
April 20, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28410121/nitric-oxide-signaling-during-meiotic-cell-cycle-regulation-in-mammalian-oocytes
#3
Meenakshi Tiwari, Shilpa Prasad, Ashutosh N Pandey, Karuppanan V Premkumar, Anima Tripathi, Anumegha Gupta, Doddalingaiah R Chetan, Pramod K Yadav, Tulsidas G Shrivastav, Shail K Chaube
  Nitric oxide (NO) acts as a major signal molecules and modulate physiology of mammalian oocytes. Ovarian follicles generate large amount of NO through nitric oxide synthase (NOS) pathway to maintain diplotene arrest in preovulatory oocytes. Removal of oocytes from follicular microenvironment or follicular rupture during ovulation disrupt the flow of NO from granulosa cells to the oocyte that results a transient decrease of oocyte cytoplasmic NO level. Decreased NO level reduces cyclic nucleotides level by inactivating guanylyl cyclases directly or indirectly...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28400616/granulosa-cell-endothelin-2-expression-is-fundamental-for-ovulatory-follicle-rupture
#4
Joseph A Cacioppo, Po-Ching Patrick Lin, Patrick R Hannon, Daniel R McDougle, Arnon Gal, CheMyong Ko
Ovulation is dependent upon numerous factors mediating follicular growth, vascularization, and ultimately oocyte release via follicle rupture. Endothelin-2 (EDN2) is a potent vasoconstrictor that is transiently produced prior to follicle rupture by granulosa cells of periovulatory follicles and induces ovarian contraction. To determine the role of Edn2 expression, surgical transplant and novel conditional knockout mice were super-ovulated and analyzed. Conditional knockout mice utilized a new iCre driven by the Esr2 promoter to selectively remove Edn2...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28393578/kisspeptin-as-a-promising-oocyte-maturation-trigger-for-in-vitro-fertilisation-in-humans
#5
Miro Kasum, Daniela Franulić, Ermin Čehić, Slavko Orešković, Albert Lila, Emina Ejubović
The aim of this review is to analyse the effectiveness of exogenous kisspeptin administration as a novel alternative of triggering oocyte maturation, instead of currently used triggers such as human chorionic gonadotropin (hCG) or gonadotropin releasing hormone (GnRH) agonist, in women undergoing in vitro fertilisation (IVF) treatment. Kisspeptin has been considered a master regulator of two modes of GnRH and hence gonadotropin secretion, pulses and surges. Administration of kisspeptin-10 and kisspeptin-54 induces the luteinising hormone (LH) surge required for egg maturation and ovulation in animal investigations and LH release during the preovulatory phase of the menstrual cycle and hypothalamic amenorrhoea in humans...
April 10, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28386066/preovulatory-suppression-of-mouse-oocyte-cell-volume-regulatory-mechanisms-is-via-signalling-that-is-distinct-from-meiotic-arrest
#6
Samantha Richard, Jay M Baltz
GLYT1-mediated glycine transport is the main cell volume-homeostatic mechanism in mouse eggs and early preimplantation embryos. It is unique to these developmental stages and key to their healthy development. GLYT1 first becomes activated in oocytes only after ovulation is triggered, when meiotic arrest of the oocyte is released, but how this occurs was unknown. Here we show that GLYT1 activity is suppressed in oocytes in the preovulatory antral follicle and that its suppression is mediated by a mechanism distinct from the gap junction-dependent Natriuretic Peptide Precursor C (NPPC) pathway that controls meiotic arrest...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28351814/combining-several-interventions-to-reduce-the-incidence-of-ohss-a-prospective-cohort-study
#7
Mohamed Elmahdy, Eman A Abdelsalam, Hassan A Maghraby
OBJECTIVE: To assess the outcome of using low-dose aspirin, dopamine agonist and triggering ovulation by low dose of HCG in combination with GnRH agonist in fixed GnRH antagonist protocol in patients at risk of OHSS. STUDY DESIGN: This prospective cohort study was conducted on 50 infertile women who were at high risk of OHSS. They received low dose aspirin from first day of stimulation, cabergoline 0.5mg daily from the day of HCG for 8days and low dose of HCG (2500 IU) in combination with GnRH agonist for final oocyte maturation in fixed GnRH antagonist protocol...
May 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28331075/myosin-activity-drives-actomyosin-bundle-formation-and-organization-in-contractile-cells-of-the-c-elegans-spermatheca
#8
Alison C E Wirshing, Erin J Cram
Stress fibers, contractile actomyosin bundles, are important for cellular force production and adaptation to physical stress and have been well-studied within the context of cell migration. However, less is known about actomyosin bundle formation and organization in vivo and in specialized contractile cells, such as smooth muscle and myoepithelial cells. The Caenorhabditis elegans spermatheca is a bag-like organ of 24 myoepithelial cells that houses the sperm and is the site of fertilization. During ovulation, spermathecal cells are stretched by oocyte entry and then coordinately contract to expel the fertilized embryo into the uterus...
March 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28283264/uterine-contractility-and-elastography-as-prognostic-factors%C3%A2-for-pregnancy-after-intrauterine-insemination
#9
Nelly Swierkowski-Blanchard, Florence Boitrelle, Laura Alter, Jacqueline Selva, Thibaud Quibel, Antoine Torre
OBJECTIVE: To determine the impact of the frequency and intensity of uterine contractions (UCs) at the time of IUI on subsequent fertility. DESIGN: Observational pilot study. SETTING: University hospital. PATIENT(S): One hundred volunteer women scheduled for IUI between April 2011 and July 2013, in whom UCs were assessed during the ultrasound before IUI. INTERVENTION(S): A two-dimensional sagittal uterus elastography was recorded for 5 minutes...
April 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28280800/clomiphene-citrate-versus-letrozole-with-gonadotropins-in-intrauterine-insemination-cycles-a-randomized-trial
#10
Leila Pourali, Sedigheh Ayati, Shirin Tavakolizadeh, Hourieh Soleimani, Fatemeh Teimouri Sani
BACKGROUND: Clomiphene citrate is one of the effective drugs for infertility treatment due to oligo-ovulation or anovulation. Intrauterine insemination (IUI) is one of more adherent methods for treatment of infertile cases which is followed by controlled ovarian hyperstimulation (COH). OBJECTIVE: the aim of this study was to evaluate Clomiphene citrate versus letrozole with gonadotropins in IUI cycles. MATERIALS AND METHODS: In this prospective randomized trial, 180 infertile women who were referred to Milad Hospital were selected...
January 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28276192/the-pregnancy-outcome-of-progesterone-primed-ovarian-stimulation-using-medroxyprogesterone-acetate4mg-versus-10mg-daily-in-infertile-women-undergoing-in-vitro-fertilization-a-randomized-controlled-trial
#11
J Dong, Y Wang, W R Chai, Q Q Hong, N L Wang, L H Sun, H Long, L Wang, H Tian, Q F Lyu, X F Lu, Q J Chen, Y P Kuang
OBJECTIVE: To investigate the clinical outcome and endocrinological characteristics of progestin-primed ovarian stimulation (PPOS) using medroxyprogesterone acetate (MPA) 4mg versus 10mg daily in infertile women with normal ovary reserve. DESIGN: A randomized parallel controlled trial. SETTING: Tertiary-care academic medical center. PARTICIPANTS: 300 infertile women undergoing IVF/ICSI treatment. METHODS: HMG 225IU/d and MPA (group A: 10mg/d; group B: 4mg/d) were started simultaneously from cycle day 3 onwards...
March 9, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28272720/the-efficacy-of-modified-luteal-phase-support-with-intramuscular-progesterone-in-ivf-icsi-cycles-a-retrospective-observational-study
#12
A Conforti, I Strina, A Mollo, R Amoroso, V Marrone, C Alviggi, R Marci, G de Placido
OBJECTIVE: The use of gonadotropin-releasing hormone agonist for ovulation triggering has become an intriguing topic in the last few years. As long as adequate luteal phase support is provided, it may be a valuable alternative to standard hCG triggering, associated with a significant reduction in OHSS incidence. Several luteal phase support options have been proposed, but few studies have addressed the issue of the appropriate route for progesterone administration to women triggered with GnRHa...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28231273/periovulatory-follicular-fluid-levels-of-estradiol-trigger-inflammatory-and-dna-damage-responses-in-oviduct-epithelial-cells
#13
Sergio E Palma-Vera, Jennifer Schoen, Shuai Chen
OBJECTIVE: Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models...
2017: PloS One
https://www.readbyqxmd.com/read/28203244/pistil-transcriptome-analysis-to-disclose-genes-and-gene-products-related-to-aposporous-apomixis-in-hypericum-perforatum-l
#14
Giulio Galla, Sara Zenoni, Linda Avesani, Lothar Altschmied, Paride Rizzo, Timothy F Sharbel, Gianni Barcaccia
Unlike sexual reproduction, apomixis encompasses a number of reproductive strategies, which permit maternal genome inheritance without genetic recombination and syngamy. The key biological features of apomixis are the circumvention of meiosis (i.e., apomeiosis), the differentiation of unreduced embryo sacs and egg cells, and their autonomous development in functional embryos through parthenogenesis, and the formation of viable endosperm either via fertilization-independent means or following fertilization with a sperm cell...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28197932/effect-of-gnrha-ovulation-trigger-dose-on-follicular-fluid-characteristics-and-granulosa-cell-gene-expression-profiles
#15
Thi Ngoc Lan Vuong, M T Ho, T Q Ha, M Brehm Jensen, C Yding Andersen, P Humaidan
PURPOSE: A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and top-quality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression...
April 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28098283/high-precision-localized-proton-gradients-and-fluxes-generated-by-a-microelectrode-device-induce-differential-growth-behaviors-of-pollen-tubes
#16
Chengzhi Hu, Hannes Vogler, Marianne Aellen, Naveen Shamsudhin, Bumjin Jang, Jan T Burri, Nino Läubli, Ueli Grossniklaus, Salvador Pané, Bradley J Nelson
Pollen tubes are tip-growing plant cells that deliver the sperm cells to the ovules for double fertilization of the egg cell and the endosperm. Various directional cues can trigger the reorientation of pollen tube growth direction on their passage through the female tissues. Among the external stimuli, protons serve an important, regulatory role in the control of pollen tube growth. The generation of local guidance cues has been challenging when investigating the mechanisms of perception and processing of such directional triggers in pollen tubes...
January 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28090464/the-efficacy-of-intrauterine-instillation-of-granulocyte-colony-stimulating-factor-in-infertile-women-with-a-thin-endometrium-a-pilot-study
#17
Dayong Lee, Jae Dong Jo, Seul Ki Kim, Byung Chul Jee, Seok Hyun Kim
OBJECTIVE: The study aimed to investigate the efficacy of intrauterine instillation of granulocyte colony-stimulating factor (G-CSF) on the day of ovulation triggering or oocyte retrieval in infertile women with a thin endometrium. METHODS: Fifty women whose endometrial thickness (EMT) was ≤8 mm at the time of triggering during at least one previous in vitro fertilization (IVF) cycle and an index IVF cycle were selected. On the day of triggering (n=12) or oocyte retrieval (n=38), 300 µg of G-CSF was instilled into the uterine cavity...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28029396/standards-of-care-in-infertility-in-europe
#18
REVIEW
Christina I Messini, Alexandros Daponte, George Anifandis, Tahir Mahmood, Ioannis E Messinis
Evidence-based medicine is the principal approach to medical practice. There are several debatable issues in infertility, which require clarification. Over the past 20 years, reliable methodology has been developed for the management of infertile couples. This includes high quality diagnostic and therapeutic procedures, which are applied in highly specialised infertility centres. The European Board and College of Obstetrics and Gynaecology (EBCOG) has published Standards of Care for Women's Health in Europe, which should be the cornerstone for the clinicians and service providers in the European Union to establish common protocols within their centres...
October 20, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27999090/biologically-active-recombinant-carp-lh-as-a-spawning-inducing-agent-for-carp
#19
Joseph Aizen, Lian Hollander-Cohen, Michal Shpilman, Berta Levavi-Sivan
Currently spawning induction in carp species uses carp pituitary extract (CPE) and a combination of synthetic agonist of GnRH -combined with a dopamine antagonist. The main goal of this study was the production of recombinant gonadotropins (GtHs) on a large scale to serve as an alternative to currently used agents. We produced carp (c) recombinant (r) LH as a single-chain in the methylotrophic yeast P. pastoris. LHα-subunit was joined with LHβ-subunit with a flexible linker of three Glycine-Serine repeats and six Histidines to form a mature protein, the β-subunit formed the N-terminal part and the α-subunit the C-terminal part...
December 20, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27986819/there-is-no-evidence-that-the-time-from-egg-retrieval-to-embryo-transfer-affects-live-birth-rates-in-a-freeze-all-strategy
#20
K Lattes, M A Checa, R Vassena, M Brassesco, V Vernaeve
STUDY QUESTION: Does the time from ovum pick-up (OPU) to frozen embryo transfer (FET) affect reproductive outcomes in a freeze-all strategy? SUMMARY ANSWER: Our study did not detect statistically significant differences between first and subsequent cycles, clinically relevant differences are not ruled out and further and larger studies are required. WHAT IS KNOWN ALREADY: Following controlled ovarian hyperstimulation (COH) delaying FET until the endometrium has returned to an optimal pre-stimulation state may have a significant emotional impact on patients, which adds to the stress and anxiety accompanying a standard IVF cycle...
February 2017: Human Reproduction
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