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https://www.readbyqxmd.com/read/28283264/uterine-contractility-and-elastography-as-prognostic-factors%C3%A2-for-pregnancy-after-intrauterine-insemination
#1
Nelly Swierkowski-Blanchard, Florence Boitrelle, Laura Alter, Jacqueline Selva, Thibaud Quibel, Antoine Torre
OBJECTIVE: To determine the impact of the frequency and intensity of uterine contractions (UCs) at the time of IUI on subsequent fertility. DESIGN: Observational pilot study. SETTING: University hospital. PATIENT(S): One hundred volunteer women scheduled for IUI between April 2011 and July 2013, in whom UCs were assessed during the ultrasound before IUI. INTERVENTION(S): A two-dimensional sagittal uterus elastography was recorded for 5 minutes...
March 7, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28280800/clomiphene-citrate-versus-letrozole-with-gonadotropins-in-intrauterine-insemination-cycles-a-randomized-trial
#2
Leila Pourali, Sedigheh Ayati, Shirin Tavakolizadeh, Hourieh Soleimani, Fatemeh Teimouri Sani
BACKGROUND: Clomiphene citrate is one of the effective drugs for infertility treatment due to oligo-ovulation or anovulation. Intrauterine insemination (IUI) is one of more adherent methods for treatment of infertile cases which is followed by controlled ovarian hyperstimulation (COH). OBJECTIVE: the aim of this study was to evaluate Clomiphene citrate versus letrozole with gonadotropins in IUI cycles. MATERIALS AND METHODS: In this prospective randomized trial, 180 infertile women who were referred to Milad Hospital were selected...
January 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28276192/the-pregnancy-outcome-of-progesterone-primed-ovarian-stimulation-using-medroxyprogesterone-acetate4mg-versus-10mg-daily-in-infertile-women-undergoing-in-vitro-fertilization-a-randomized-controlled-trial
#3
J Dong, Y Wang, W R Chai, Q Q Hong, N L Wang, L H Sun, H Long, L Wang, H Tian, Q F Lyu, X F Lu, Q J Chen, Y P Kuang
OBJECTIVE: To investigate the clinical outcome and endocrinological characteristics of progestin-primed ovarian stimulation (PPOS) using medroxyprogesterone acetate (MPA) 4mg versus 10mg daily in infertile women with normal ovary reserve. DESIGN: A randomized parallel controlled trial. SETTING: Tertiary-care academic medical center. PARTICIPANTS: 300 infertile women undergoing IVF/ICSI treatment. METHODS: HMG 225IU/d and MPA (group A: 10mg/d; group B: 4mg/d) were started simultaneously from cycle day 3 onwards...
March 9, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28272720/the-efficacy-of-modified-luteal-phase-support-with-intramuscular-progesterone-in-ivf-icsi-cycles-a-retrospective-observational-study
#4
A Conforti, I Strina, A Mollo, R Amoroso, V Marrone, C Alviggi, R Marci, G de Placido
OBJECTIVE: The use of gonadotropin-releasing hormone agonist for ovulation triggering has become an intriguing topic in the last few years. As long as adequate luteal phase support is provided, it may be a valuable alternative to standard hCG triggering, associated with a significant reduction in OHSS incidence. Several luteal phase support options have been proposed, but few studies have addressed the issue of the appropriate route for progesterone administration to women triggered with GnRHa...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28231273/periovulatory-follicular-fluid-levels-of-estradiol-trigger-inflammatory-and-dna-damage-responses-in-oviduct-epithelial-cells
#5
Sergio E Palma-Vera, Jennifer Schoen, Shuai Chen
OBJECTIVE: Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models...
2017: PloS One
https://www.readbyqxmd.com/read/28203244/pistil-transcriptome-analysis-to-disclose-genes-and-gene-products-related-to-aposporous-apomixis-in-hypericum-perforatum-l
#6
Giulio Galla, Sara Zenoni, Linda Avesani, Lothar Altschmied, Paride Rizzo, Timothy F Sharbel, Gianni Barcaccia
Unlike sexual reproduction, apomixis encompasses a number of reproductive strategies, which permit maternal genome inheritance without genetic recombination and syngamy. The key biological features of apomixis are the circumvention of meiosis (i.e., apomeiosis), the differentiation of unreduced embryo sacs and egg cells, and their autonomous development in functional embryos through parthenogenesis, and the formation of viable endosperm either via fertilization-independent means or following fertilization with a sperm cell...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28197932/effect-of-gnrha-ovulation-trigger-dose-on-follicular-fluid-characteristics-and-granulosa-cell-gene-expression-profiles
#7
Thi Ngoc Lan Vuong, M T Ho, T Q Ha, M Brehm Jensen, C Yding Andersen, P Humaidan
PURPOSE: A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and top-quality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression...
February 14, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28098283/high-precision-localized-proton-gradients-and-fluxes-generated-by-a-microelectrode-device-induce-differential-growth-behaviors-of-pollen-tubes
#8
Chengzhi Hu, Hannes Vogler, Marianne Aellen, Naveen Shamsudhin, Bumjin Jang, Jan T Burri, Nino Läubli, Ueli Grossniklaus, Salvador Pané, Bradley J Nelson
Pollen tubes are tip-growing plant cells that deliver the sperm cells to the ovules for double fertilization of the egg cell and the endosperm. Various directional cues can trigger the reorientation of pollen tube growth direction on their passage through the female tissues. Among the external stimuli, protons serve an important, regulatory role in the control of pollen tube growth. The generation of local guidance cues has been challenging when investigating the mechanisms of perception and processing of such directional triggers in pollen tubes...
January 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28090464/the-efficacy-of-intrauterine-instillation-of-granulocyte-colony-stimulating-factor-in-infertile-women-with-a-thin-endometrium-a-pilot-study
#9
Dayong Lee, Jae Dong Jo, Seul Ki Kim, Byung Chul Jee, Seok Hyun Kim
OBJECTIVE: The study aimed to investigate the efficacy of intrauterine instillation of granulocyte colony-stimulating factor (G-CSF) on the day of ovulation triggering or oocyte retrieval in infertile women with a thin endometrium. METHODS: Fifty women whose endometrial thickness (EMT) was ≤8 mm at the time of triggering during at least one previous in vitro fertilization (IVF) cycle and an index IVF cycle were selected. On the day of triggering (n=12) or oocyte retrieval (n=38), 300 µg of G-CSF was instilled into the uterine cavity...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28029396/standards-of-care-in-infertility-in-europe
#10
REVIEW
Christina I Messini, Alexandros Daponte, George Anifandis, Tahir Mahmood, Ioannis E Messinis
Evidence-based medicine is the principal approach to medical practice. There are several debatable issues in infertility, which require clarification. Over the past 20 years, reliable methodology has been developed for the management of infertile couples. This includes high quality diagnostic and therapeutic procedures, which are applied in highly specialised infertility centres. The European Board and College of Obstetrics and Gynaecology (EBCOG) has published Standards of Care for Women's Health in Europe, which should be the cornerstone for the clinicians and service providers in the European Union to establish common protocols within their centres...
October 20, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27999090/biologically-active-recombinant-carp-lh-as-a-spawning-inducing-agent-for-carp
#11
Joseph Aizen, Lian Hollander-Cohen, Michal Shpilman, Berta Levavi-Sivan
Currently spawning induction in carp species uses carp pituitary extract (CPE) and a combination of synthetic agonist of GnRH -combined with a dopamine antagonist. The main goal of this study was the production of recombinant gonadotropins (GtHs) on a large scale to serve as an alternative to currently used agents. We produced carp (c) recombinant (r) LH as a single-chain in the methylotrophic yeast P. pastoris. LHα-subunit was joined with LHβ-subunit with a flexible linker of three Glycine-Serine repeats and six Histidines to form a mature protein, the β-subunit formed the N-terminal part and the α-subunit the C-terminal part...
December 20, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27986819/there-is-no-evidence-that-the-time-from-egg-retrieval-to-embryo-transfer-affects-live-birth-rates-in-a-freeze-all-strategy
#12
K Lattes, M A Checa, R Vassena, M Brassesco, V Vernaeve
STUDY QUESTION: Does the time from ovum pick-up (OPU) to frozen embryo transfer (FET) affect reproductive outcomes in a freeze-all strategy? SUMMARY ANSWER: Our study did not detect statistically significant differences between first and subsequent cycles, clinically relevant differences are not ruled out and further and larger studies are required. WHAT IS KNOWN ALREADY: Following controlled ovarian hyperstimulation (COH) delaying FET until the endometrium has returned to an optimal pre-stimulation state may have a significant emotional impact on patients, which adds to the stress and anxiety accompanying a standard IVF cycle...
February 2017: Human Reproduction
https://www.readbyqxmd.com/read/27975269/egg-activation-at-fertilization
#13
Zoltan Machaty, Andrew R Miller, Lu Zhang
Fertilization is the union of gametes to initiate development of a new individual. The female gamete is formed during oogenesis. The process begins when, in the early embryo, primordial germ cells arise and subsequently colonize the genital ridges. They differentiate into oogonia, start meiosis, and become primary oocytes. The cell cycle of the primary oocytes then becomes arrested in mid-meiosis for an extended period of time. Prior to ovulation the oocytes undergo a growth phase and their sizes increase significantly...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27865446/use-of-utrogestan-during-controlled-ovarian-hyperstimulation-in-normally-ovulating-women-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection-treatments-in-combination-with-a-freeze-all-strategy-a-randomized-controlled-dose-finding-study-of
#14
Xiuxian Zhu, Hongjuan Ye, Yonglun Fu
OBJECTIVE: To compare the clinical characteristics in a Utrogestan and hMG protocol with the use of different doses of Utrogestan in normally ovulating women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatments. DESIGN: Prospective controlled study. SETTING: Tertiary-care academic medical center. PATIENT(S): A total of 150 infertile patients undergoing IVF/ICSI treatments. INTERVENTION(S): Utrogestan and hMG were administered simultaneously beginning on cycle day 3...
February 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27809622/nitric-oxide-donors-increase-the-pregnancy-rate-in-patients-with-unexplained-infertility-undergoing-clomiphene-citrate-stimulation-and-intrauterine-insemination-a-randomized-controlled-pilot-study
#15
Mohamed Abdel Razik, Seham El-Berry, Ahmed El-Nezamy, Ahmed Saad, Ahmed Abdel Wahab
This study evaluated the effects of nitric oxide donor's treatment on the pregnancy rate and uterine blood flow in patients with unexplained infertility undergoing clomiphene citrate stimulation and intrauterine insemination. A total of 120 patients were randomly allocated to a control group who received 100 mg clomiphene citrate daily from day 5 to 9 of cycle plus placebo vaginal tablets, and a study group received clomiphene citrate plus isosorbide mononitrate 10 mg vaginal tablets. Vaginal ultrasound was done before treatment and every other day starting from day 12 of cycle to count mature follicles and ovulation was triggered by IM injection of 10 000 IU hCG when one follicle measured 18 ≥ mm followed by intrauterine insemination after 36 h...
November 4, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27806429/cellular-localization-of-relaxin-like-gonad-stimulating-peptide-expression-in-asterias-rubens-new-insights-into-neurohormonal-control-of-spawning-in-starfish
#16
Ming Lin, Masatoshi Mita, Michaela Egertová, Cleidiane G Zampronio, Alexandra M Jones, Maurice R Elphick
Gamete maturation and spawning in starfish is triggered by a gonad-stimulating substance (GSS), which is present in extracts of the radial nerve cords. Purification of GSS from the starfish Patiria pectinifera identified GSS as a relaxin-like polypeptide, which is now known as relaxin-like gonad-stimulating peptide (RGP). Cells expressing RGP in the radial nerve cord of P. pectinifera have been visualized, but the presence of RGP-expressing cells in other parts of the starfish body has not been investigated...
May 1, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/27798046/frozen-thawed-embryo-transfers-in-natural-cycles-with-spontaneous-or-induced-ovulation-the-search-for-the-best-protocol-continues
#17
M Montagut, S Santos-Ribeiro, M De Vos, N P Polyzos, P Drakopoulos, S Mackens, A van de Vijver, L van Landuyt, G Verheyen, H Tournaye, C Blockeel
STUDY QUESTION: Is natural cycle frozen-thawed embryo transfer (NC-FET) associated with better clinical pregnancy rates (CPR) when compared to modified natural cycle frozen-thawed embryo transfer (mNC-FET)? SUMMARY ANSWER: NC-FET is associated with a higher CPR compared to mNC-FET. WHAT IS KNOWN ALREADY: There is conflicting evidence regarding the impact of hCG triggering on clinical outcomes after frozen-thawed embryo transfer (FET), and information on the effect of luteal phase support (LPS) is lacking...
December 2016: Human Reproduction
https://www.readbyqxmd.com/read/27774052/altered-expression-of-genes-encoding-neurotransmitter-receptors-in-gnrh-neurons-of-proestrous-mice
#18
Csaba Vastagh, Annie Rodolosse, Norbert Solymosi, Zsolt Liposits
Gonadotropin-releasing hormone (GnRH) neurons play a key role in the central regulation of reproduction. In proestrous female mice, estradiol triggers the pre-ovulatory GnRH surge, however, its impact on the expression of neurotransmitter receptor genes in GnRH neurons has not been explored yet. We hypothesized that proestrus is accompanied by substantial changes in the expression profile of genes coding for neurotransmitter receptors in GnRH neurons. We compared the transcriptome of GnRH neurons obtained from intact, proestrous, and metestrous female GnRH-GFP transgenic mice, respectively...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27770611/creb-activity-is-required-for-luteinizing-hormone-induced-the-expression-of-egf-like-factors
#19
Yakun Wang, Xiaoqiong Hao, Jing Yang, Jia Li, Meijia Zhang
A surge of luteinizing hormone (LH) from the pituitary gland induces the expression of the epidermal growth factor (EGF)-like factors, which triggers oocyte maturation, cumulus expansion, and ovulation. How LH induces EGF-like factor expression is unclear. In the present study, a rapid increase of phosphorylated cAMP response element binding protein (CREB) was observed after the activation of LH receptor by human chorionic gonadotropin. Large antral follicles from equine chorionic gonadotropin-primed mice were cultured in medium with LH to stimulate the expression of EGF-like factors...
December 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27738657/gnrh-agonist-trigger-versus-hcg-trigger-in-gnrh-antagonist-in-ivf-icsi-cycles-a-review-article
#20
REVIEW
Ashraf Alyasin, Shayesteh Mehdinejadiani, Marzieh Ghasemi
Routinely, a bolus of 5.000-10.000 IU human chorionic gonadotropin (hCG) is used for the final follicular maturation and ovulation as a standard method. HCG has the same effect of luteinizing hormone (LH) with long half-life. It has the long lutheotrophic effect which increases the risk of ovarian hyper stimulation syndrome (OHSS). Recently, gonadotropin-releasing hormone agonist (GnRH-a) trigger has been used for the induction of final follicular maturation and ovulation with the aim of reducing the OHSS risk...
September 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
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