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Gyanendra Kumar

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https://www.readbyqxmd.com/read/27866915/trends-in-transcranial-doppler-monitoring-in-aneurysmal-subarachnoid-hemorrhage-a-10-year-analysis-of-the-nationwide-inpatient-sample
#1
Gyanendra Kumar, Karen C Albright, John P Donnelly, Angela Hays Shapshak, Mark R Harrigan
BACKGROUND: Transcranial Doppler (TCD) is endorsed by national guidelines for use in aneurysmal subarachnoid hemorrhage (aSAH) for surveillance of cerebral vasospasm (CV). However, nationwide data on utilization of TCD for CV detection and monitoring in aSAH are lacking. METHODS: Analysis of nationwide trends in TCD prevalence was performed using Nationwide Inpatient Sample (NIS) data from 2002 to 2011. Raw counts were converted into weighted counts, which were used to generate national estimates...
November 17, 2016: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/27866718/mutational-analysis-of-metastatic-lymph-nodes-from-papillary-thyroid-carcinoma-in-adult-and-pediatric-patients
#2
Alexander L Shifrin, Michele Fischer, Trevor Paul, Brian Erler, Katherine Gheysens, Prachi Baodhankar, Joanna W Song-Yang, Samantha Taylor, Venkata Arun Timmaraju, Arthur Topilow, Alidad Mireskandari, Gyanendra Kumar
BACKGROUND: Limited data are available on the analysis of somatic mutations in metastatic lymph nodes in adult and pediatric patients with papillary thyroid carcinomas. METHODS: A total of 92, microdissected, formalin-fixed, paraffin-embedded tissue specimens from 39 patients were analyzed for the presence of somatic mutations utilizing the ThyGenX next-generation sequencing test. RESULTS: Somatic mutations were detected in 67% of papillary thyroid carcinoma specimens...
November 17, 2016: Surgery
https://www.readbyqxmd.com/read/27857501/prospective-study-of-the-anterior-cruciate-ligament-reconstruction-associated-with-high-tibial-opening-wedge-osteotomy-in-knee-arthritis-associated-with-instability
#3
Raju Vaishya, Vipul Vijay, Gyanendra Kumar Jha, Amit Kumar Agarwal
INTRODUCTION: Chronic ACL insufficiency with associated varus malalignment due to knee osteoarthritis (OA) is challenging to treat surgically. A combined ACL reconstruction (ACLR) with medial open wedge high tibial osteotomy (HTO) without using any metallic implant for HTO is an effective technique. MATERIALS AND METHOD: All the patients attending the outpatient department ACL injury and with associated medial compartment OA (Kellegren's grade 2 and grade 3) were considered for inclusion in the study...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/27853874/comparative-expression-profile-of-nod1-2-and-certain-acute-inflammatory-cytokines-in-thermal-stressed-cell-culture-model-of-native-and-crossbred-cattle
#4
V Bhanuprakash, Umesh Singh, Gyanendra Singh Sengar, T V Raja, Basavraj Sajjanar, Rani Alex, Sushil Kumar, R R Alyethodi, Ashish Kumar, Ankur Sharma, Suresh Kumar, Bharat Bhusan, Rajib Deb
Thermotolerance depends mainly on the health and immune status of the animals. The variation in the immune status of the animals may alter the level of tolerance of animals exposed to heat or cold stress. The present study was conducted to investigate the expression profile of two important nucleotide binding and oligomerization domain receptors (NLRs) (NOD1 and NOD2) and their central signalling molecule RIP2 gene during in vitro thermal-stressed bovine peripheral blood mononuclear cells (PBMCs) of native (Sahiwal) and crossbred (Sahiwal X HF) cattle...
November 16, 2016: International Journal of Biometeorology
https://www.readbyqxmd.com/read/27847354/an-amino-acid-in-the-stalk-domain-of-n1-neuraminidase-is-critical-for-enzymatic-activity
#5
Mark Zanin, Susu Duan, Sook-San Wong, Gyanendra Kumar, Pradyumna Baviskar, Emily Collin, Charles Russell, Subrata Barman, Benjamin Hause, Richard Webby
: Neuraminidase (NA) is a sialidase expressed on the surface of influenza A viruses that releases progeny viruses from the surface of infected cells and prevents viruses becoming trapped in mucus. It is a homo-tetramer with each monomer consisting of a transmembrane region, a stalk and a globular head with sialidase activity. We recently characterized two swine viruses of the pandemic H1N1 lineage; A/swine/Virginia/1814-1/2012 (pH1N1low-1) and A/swine/Virginia/1814-2/2012 (pH1N1low-2) with almost undetectable NA enzymatic activity compared to the highly homologous A/swine/Pennsylvania/2436/2012 (pH1N1-1) and A/swine/Minnesota/2499/2012 (pH1N1-2) viruses...
November 9, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27761701/application-of-a-loop-mediated-isothermal-amplification-assay-for-rapid-detection-of-cow-components-adulterated-in-buffalo-milk-meat
#6
Rajib Deb, Gyanendra Singh Sengar, Umesh Singh, Sushil Kumar, R R Alyethodi, Rani Alex, T V Raja, A K Das, B Prakash
Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection of cow components adulteration in buffalo milk/meat samples. The test can be completed within around 1 h 40 min starting from DNA extraction and can be performed in water bath without requirement of thermocycler. The cow DNA in buffalo samples were identified in the developed LAMP assay by either visualizing with SYBR Green I/HNB dyes or observing the typical ladder pattern on gel electrophoresis...
October 19, 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/27751593/molecular-markers-bm1500-and-umn2008-are-associated-with-post-thaw-motility-of-bull-sperm
#7
Rafeeque R Alyethodi, Rajib Deb, Rani Alex, Sushil Kumar, Umesh Singh, Shrikant Tyagi, D K Mandal, T V Raja, A K Das, Sheetal Sharma, Gyanendra S Sengar, Rani Singh, B Prakash
Cryopreservation is one of the most important aspects of frozen semen technology and livestock breeding. Uses of candidate molecular markers in selection strategies for male fertility are well recognized. The present investigation targeted two microsatellite markers (BM1500 and UMN 2008) for association with semen quality variables and freezing capacity in Frieswal (HF×Sahiwal) crossbred bulls of Indian origin. Of the different alleles at the polymorphic locus BM1500, the 136bp allele was associated with greater (P<0...
October 11, 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27721563/comparison-of-shear-bond-strength-of-reattached-incisor-fragment-using-er-cr-ysgg-laser-etching-and-conventional-acid-etching-an-in-vitro-study
#8
Ferah Rehman, Gyanendra Kumar, Mridula Goswami, Jatinder Kaur Dhillon
Aim: The aim of this invitro study is to evaluate the shear bond strength of reattached fractured incisor fragments using Er,Cr:YSGG laser and conventional acid etching without additional tooth preparation. Materials and methods: Forty extracted human teeth were divided in two groups of 20 each (Groups A and B). In Group A, fractured surface was treated by an Er, Cr: YSGG laser system (Waterlase MD, Biolase Technology Inc., San Clemente, CA, USA) operating at a wavelength of 2,780 nm and frequency of 20 Hz...
June 29, 2016: Laser Therapy
https://www.readbyqxmd.com/read/27652018/development-of-a-fast-and-economical-genotyping-protocol-for-bovine-leukocyte-adhesion-deficiency-blad-in-cattle
#9
Rafeeque R Alyethodi, Umesh Singh, Sushil Kumar, Rajib Deb, Rani Alex, Sheetal Sharma, Gyanendra S Sengar, B Prakash
Fast and economical means of assaying SNP's are important in diagnostic assays, especially when a large number of animals have to be screened for a genetic disease. This study was aimed at the development of a fast and economical screening assay for bovine leukocyte adhesion deficiency (BLAD) which is an important genetic disease of cattle industry. Four primers were designed where the outer primers amplify a 354 bp amplicon of CD18 gene carrying the polymorphism responsible for BLAD. The specifically designed inner primers in conjunction with the modified reaction mixture and cyclic conditions ensured amplification of either of wild or mutated alleles...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27641974/risk-of-intussusception-with-rotavirus-vaccine
#10
Vivekanandan Kalaiselvan, Prasad Thota, Vipin Kumar, Madhvi Singh Rathore, Anusha Thota, Gyanendra Nath Singh
OBJECTIVE: To study the case series for intussusception associated with the vaccination of rotavirus vaccine in children. METHODS: The study of spontaneous adverse event monitoring such as intussusception due to rotavirus vaccine was carried out from the year 2011 through 2015. The individual case safety reports (ICSRs) of this event were collated, assessed and recorded as per the requirement of Suspected Adverse Drug Reactions Reporting form of Pharmacovigilance Programme of India (PvPI)...
September 19, 2016: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/27630850/prevalence-of-autoantibodies-and-hla-dr-dq-in-type-1-diabetes-mellitus
#11
Shailja Singh, Usha, Gyanendra Singh, Neeraj Kumar Agrawal, Rana Gopal Singh, Shashi Bhushan Kumar
INTRODUCTION: Type I diabetes Mellitus (T1DM) is caused by autoimmune destruction of β-cells of pancreas. Two forms of T1DM are known called as 1A (autoimmune) and 1B (idiopathic). AIM: Aim was to study the prevalence of Anti-TTG IgA, Anti-TPO, GADA, ZnT8 and IA-2 autoantibodies and HLA DR and DQ genes and its diagnostic value in T1DM. MATERIALS AND METHODS: Thirty four T1DM patients, 59 type 2 diabetes mellitus (T2DM) patients and 28 healthy controls were included in study...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27582756/ssh-analysis-of-endosperm-transcripts-and-characterization-of-heat-stress-regulated-expressed-sequence-tags-in-bread-wheat
#12
Suneha Goswami, Ranjeet R Kumar, Kavita Dubey, Jyoti P Singh, Sachidanand Tiwari, Ashok Kumar, Shuchi Smita, Dwijesh C Mishra, Sanjeev Kumar, Monendra Grover, Jasdeep C Padaria, Yugal K Kala, Gyanendra P Singh, Himanshu Pathak, Viswanathan Chinnusamy, Anil Rai, Shelly Praveen, Raj D Rai
Heat stress is one of the major problems in agriculturally important cereal crops, especially wheat. Here, we have constructed a subtracted cDNA library from the endosperm of HS-treated (42°C for 2 h) wheat cv. HD2985 by suppression subtractive hybridization (SSH). We identified ~550 recombinant clones ranging from 200 to 500 bp with an average size of 300 bp. Sanger's sequencing was performed with 205 positive clones to generate the differentially expressed sequence tags (ESTs). Most of the ESTs were observed to be localized on the long arm of chromosome 2A and associated with heat stress tolerance and metabolic pathways...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27510745/n-acylhydrazone-inhibitors-of-influenza-virus-pa-endonuclease-with-versatile-metal-binding-modes
#13
Mauro Carcelli, Dominga Rogolino, Anna Gatti, Laura De Luca, Mario Sechi, Gyanendra Kumar, Stephen W White, Annelies Stevaert, Lieve Naesens
Influenza virus PA endonuclease has recently emerged as an attractive target for the development of novel antiviral therapeutics. This is an enzyme with divalent metal ion(s) (Mg(2+) or Mn(2+)) in its catalytic site: chelation of these metal cofactors is an attractive strategy to inhibit enzymatic activity. Here we report the activity of a series of N-acylhydrazones in an enzymatic assay with PA-Nter endonuclease, as well as in cell-based influenza vRNP reconstitution and virus yield assays. Several N-acylhydrazones were found to have promising anti-influenza activity in the low micromolar concentration range and good selectivity...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27462325/identification-of-putative-rubisco-activase-tarca1-the-catalytic-chaperone-regulating-carbon-assimilatory-pathway-in-wheat-triticum-aestivum-under-the-heat-stress
#14
Ranjeet R Kumar, Suneha Goswami, Khushboo Singh, Kavita Dubey, Shweta Singh, Renu Sharma, Neeraj Verma, Yugal K Kala, Gyanendra K Rai, Monendra Grover, Dwijesh C Mishra, Bhupinder Singh, Himanshu Pathak, Viswanathan Chinnusamy, Anil Rai, Shelly Praveen
RuBisCo activase (Rca) is a catalytic chaperone involved in modulating the activity of RuBisCo (key enzyme of photosynthetic pathway). Here, we identified eight novel transcripts from wheat through data mining predicted to be Rca and cloned a transcript of 1.4 kb from cv. HD2985, named as TaRca1 (GenBank acc. no. KC776912). Single copy number of TaRca1 was observed in wheat genome. Expression analysis in diverse wheat genotypes (HD2985, Halna, PBW621, and HD2329) showed very high relative expression of TaRca1 in Halna under control and HS-treated, as compared to other cultivars at different stages of growth...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27353886/small-molecule-non-peptide-inhibitors-of-botulinum-neurotoxin-serotype-e-structure-activity-relationship-and-a-pharmacophore-model
#15
Gyanendra Kumar, Rakhi Agarwal, Subramanyam Swaminathan
Botulinum neurotoxins (BoNTs) are the most poisonous biological substance known to humans. They cause flaccid paralysis by blocking the release of acetylcholine at the neuromuscular junction. Here, we report a number of small molecule non-peptide inhibitors of BoNT serotype E. The structure-activity relationship and a pharmacophore model are presented. Although non-peptidic in nature, these inhibitors mimic key features of the uncleavable substrate peptide Arg-Ile-Met-Glu (RIME) of the SNAP-25 protein. Among the compounds tested, most of the potent inhibitors bear a zinc-chelating moiety connected to a hydrophobic and aromatic moiety through a carboxyl or amide linker...
September 15, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27342075/jejunal-perforation-due-to-ingested-buffalo-bone-mimicking-acute-appendicitis
#16
Ajay Kumar Yadav, Gyanendra Malla, Kunal Bikram Deo, Saroj Giri, Bishnu Murti Bhattarai, Shailesh Adhikary
BACKGROUND: Foreign body ingestion is seen quite frequently in clinical practice, intestinal perforation due to this is rare. The foreign body often mimics another cause of acute abdomen and requires an emergency surgical intervention. The majority of patients do not recall ingesting sharp foreign bodies. CASE PRESENTATION: We report an interesting case of a fifty five year-old man who presented with pain in the right iliac fossa with localised tenderness which was clinically diagnosed as acute appendicitis...
2016: BMC Research Notes
https://www.readbyqxmd.com/read/27331368/time-to-analgesia-in-an-emergency-department-in-eastern-nepal
#17
Rabin Bhandari, Gyanendra Malla, Bijendra Kumar Rai, Chris Curry
OBJECTIVES: Nepal is a least developed country, with limited healthcare resources. An 18 month Fellowship in Emergency Medicine has contributed some improvements to care. This study assessed time to first analgesia in higher and lower acuity patients. METHODS: A prospective observational study of 101 patients in each of the Australasian Triage Scale (ATS) 2, 3 and 100 in ATS 4 was undertaken at B.P. Koirala Institute of Health Sciences. Convenience sampling was used coinciding with researcher's duty hours...
August 2016: Emergency Medicine Australasia: EMA
https://www.readbyqxmd.com/read/27300000/effect-of-recurrent-selection-on-drought-tolerance-and-related-morpho-physiological-traits-in-bread-wheat
#18
Ramya P, Gyanendra Pratap Singh, Neelu Jain, Pradeep Kumar Singh, Manoj Kumar Pandey, Kavita Sharma, Arun Kumar, Harikrishna, Kumble Vinod Prabhu
Drought is one of the major abiotic stresses affecting wheat yield. A recurrent selection program was conducted to improve the drought tolerance and yield of bread wheat using drought tolerant advanced breeding lines from a drought tolerant x susceptible cross (HI 1500 x HUW 510). The parental lines were evaluated for yield, biomass and harvest index (HI) in addition to the drought adaptive traits like Canopy Temperature (CT), chlorophyll content and Normalized Difference Vegetative Index (NDVI). After three rounds of recurrent selection, the half sib progenies exhibited a marked reduction in CT, chlorophyll content and biomass; whereas improvement was noted for yield, HI and NDVI...
2016: PloS One
https://www.readbyqxmd.com/read/27264883/differential-effect-of-thermal-stress-on-hsp70-expression-nitric-oxide-production-and-cell-proliferation-among-native-and-crossbred-dairy-cattle
#19
V Bhanuprakash, Umesh Singh, Gyanendra Sengar, Basavaraj Sajjanar, Bharat Bhusan, T V Raja, Rani Alex, Sushil Kumar, Rani Singh, Ashish Kumar, R R Alyethodi, Suresh Kumar, Rajib Deb
In a tropical country like India, thermal stress is one of the major factors which significantly affects the productivity of dairy cattle. The present study was aimed to identify the effect of heat and cold stress on cell viability, mitogen stimulation indices, nitric oxide production and HSP70 expression in Sahiwal and Holstein crossbred (Frieswal) population in India. The results indicated that the Sahiwal breed can better withstand the effect of heat and cold stress significantly (P<0.05) when compared to the crossbred cattle due to the higher survivability of the Peripheral Blood Mononuclear Cells (PBMCs) and Phytohemagglutinin (PHA-P) mitogen based stimulation indices...
July 2016: Journal of Thermal Biology
https://www.readbyqxmd.com/read/27229302/effectiveness-of-a-cooling-jacket-with-reference-to-physiological-responses-in-iron-foundry-workers
#20
Ashtekar Shirish, Vishal Kapadia, Sanjeev Kumar, Sunil Kumar, Sukhdev Mishra, Gyanendra Singh
Personal cooling garments (PCGs) have gained increased attention in recent years due to heat stress and strain in the working environment. The present study was conducted in hot environments of an iron foundry to evaluate the efficacy of a battery-operated PCG. Twenty-four workers were exposed to climatic conditions of 35.89 ± 1.25 °C, 35% relative humidity during 90-min work with PCG and habitual clothing (HC). Mean weighted skin temperature was significantly lower by 4.84 ± 1.05 °C compared with HC 0...
May 27, 2016: International Journal of Occupational Safety and Ergonomics: JOSE
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