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Minsheng You

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https://www.readbyqxmd.com/read/29620473/selecting-and-validating-reference-genes-for-quantitative-real-time-pcr-in-i-plutella-xylostella-i-l
#1
Yanchun You, Miao Xie, Liette Vasseur, Minsheng You
Gene expression analysis provides important clues regarding gene functions, and quantitative real-time PCR (qRT-PCR) is a widely-used method for gene expression studies. Reference genes are essential for normalizing and accurately assessing gene expressions. In the present study, 16 candidate reference genes (<i>ACTB</i>, <i>CyPA</i>, <i>EF1-α</i>, <i>GAPDH</i>, <i>HSP90</i>, <i>NDPk</i>, <i>RPL13a</i>, <i>RPL18</i>, <i>RPL19</i>, <i>RPL32</i>, <i>RPL4</i>, <i>RPL8</i>, <i>RPS13</i>, <i>RPS4</i>, <i>α-TUB</i> and <i>β-TUB</i>) of <i>Plutella xylostella</i> were selected to evaluate their gene expression stability across different experimental conditions using five statistical algorithms (geNorm, NormFinder, Delta Ct method, BestKeeper and RefFinder)...
April 5, 2018: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29499842/a-two-step-purification-strategy-using-calmodulin-as-an-affinity-tag
#2
Lianyun Lin, Chen Liu, Bidhan Chandra Nayak, Weiyi He, Minsheng You, Zhiguang Yuchi
Calmodulin (CaM) is a Ca2+ -binding protein that plays an important role in cellular Ca2+ -signaling. CaM interacts with diverse downstream target proteins and regulates their functions in a Ca2+ -dependent manner. CaM changes its conformation and hydrophobicity upon [Ca2+ ] change and consequently changes its interaction with CaM-binding domains from the targets. Based on these special properties of CaM, it was used as an affinity tag to develop a novel purification strategy by using it for two sequential orthogonal purification steps: 1) an affinity purification step, in which CaM-tag interacts with an immobilized CaM-binding domain; and 2) a hydrophobic interaction chromatography step, during which CaM binds to a phenyl sepharose column...
February 27, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29410659/gut-microbiota-mediate-insecticide-resistance-in-the-diamondback-moth-plutella-xylostella-l
#3
Xiaofeng Xia, Botong Sun, Geoff M Gurr, Liette Vasseur, Minqian Xue, Minsheng You
The development of insecticide resistance in insect pests is a worldwide concern and elucidating the underlying mechanisms is critical for effective crop protection. Recent studies have indicated potential links between insect gut microbiota and insecticide resistance and these may apply to the diamondback moth, Plutella xylostella (L.), a globally and economically important pest of cruciferous crops. We isolated Enterococcus sp. (Firmicutes), Enterobacter sp. (Proteobacteria), and Serratia sp. (Proteobacteria) from the guts of P...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29305978/gene-expression-profiling-provides-insights-into-the-immune-mechanism-of-plutella-xylostella-midgut-to-microbial-infection
#4
Junhan Lin, Xiaofeng Xia, Xiao-Qiang Yu, Jinhong Shen, Yong Li, Hailan Lin, Shanshan Tang, Liette Vasseur, Minsheng You
Insect gut immunity plays a key role in defense against microorganism infection. The knowledge of insect gut immunity has been obtained mostly from Drosophila melanogaster. Little is known about gut immunity in the diamondback moth, Plutella xylostella (L.), a pest destroying cruciferous crops worldwide. In this study, expressions of the immune-related genes in the midgut of P. xylostella orally infected with Staphylococcus aureus, Escherichia coli and Pichia pastoris were profiled by RNA-seq and qRT-PCR approaches...
March 20, 2018: Gene
https://www.readbyqxmd.com/read/29198776/insect-c-type-lectins-in-innate-immunity
#5
Xiaofeng Xia, Minsheng You, Xiang-Jun Rao, Xiao-Qiang Yu
C-type lectins (CTLs) are a family of proteins that contain characteristic modules of carbohydrate-recognition domains (CRDs) and they possess the binding activity to ligands in a calcium-dependent manner. CTLs play important roles in animal immune responses, and in insects, they are involved in opsonization, nodule formation, agglutination, encapsulation, melanization, and prophenoloxidase activation, as well as in maintaining gut microbiome homeostasis. In this review, we will summarize insect CTLs, compare the properties of insect CTLs with vertebrate CTLs, and focus mainly on the domain organization and functions of insect CTLs in innate immunity...
December 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29191465/crystal-structure-of-ryanodine-receptor-n-terminal-domain-from-plutella-xylostella-reveals-two-potential-species-specific-insecticide-targeting-sites
#6
Lianyun Lin, Chen Liu, Juan Qin, Jie Wang, Shengjie Dong, Wei Chen, Weiyi He, Qingzhi Gao, Minsheng You, Zhiguang Yuchi
Ryanodine receptors (RyRs) are large calcium-release channels located in sarcoplasmic reticulum membrane. They play a central role in excitation-contraction coupling of muscle cells. Three commercialized insecticides targeting pest RyRs generate worldwide sales over 2 billion U.S. dollars annually, but the structure of insect RyRs remains elusive, hindering our understanding of the mode of action of RyR-targeting insecticides and the development of insecticide resistance in pests. Here we present the crystal structure of RyR N-terminal domain (NTD) (residue 1-205) at 2...
January 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29147778/genome-wide-investigation-of-transcription-factors-provides-insights-into-transcriptional-regulation-in-plutella-xylostella
#7
Qian Zhao, Dongna Ma, Yuping Huang, Weiyi He, Yiying Li, Liette Vasseur, Minsheng You
Transcription factors (TFs), which play a vital role in regulating gene expression, are prevalent in all organisms and characterization of them may provide important clues for understanding regulation in vivo. The present study reports a genome-wide investigation of TFs in the diamondback moth, Plutella xylostella (L.), a worldwide pest of crucifers. A total of 940 TFs distributed among 133 families were identified. Phylogenetic analysis of insect species showed that some of these families were found to have expanded during the evolution of P...
November 16, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28890398/functional-characterization-of-pol-iii-u6-promoters-for-gene-knockdown-and-knockout-in-plutella-xylostella
#8
Yuping Huang, Yajun Wang, Baosheng Zeng, Zhaoxia Liu, Xuejiao Xu, Qian Meng, Yongping Huang, Guang Yang, Liette Vasseur, Geoff M Gurr, Minsheng You
RNA polymerase type III (Pol-III) promoters such as U6 are commonly used to express small RNAs, including short hairpin RNAs (shRNAs) and single guide RNAs (sgRNAs). Functional U6 promoters are widely used in CRISPR systems, and their characterization can facilitate genome editing of non-model organisms. In the present study, six U6 small nuclear RNA (snRNA) promoters containing two conserved elements of a proximal sequence element (PSEA) and a TATA box, were identified and characterized in the diamondback moth (Plutella xylostella) genome...
October 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28789862/genome-wide-identification-and-characterization-of-putative-lncrnas-in-the-diamondback-moth-plutella-xylostella-l
#9
Yue Wang, Tingting Xu, Weiyi He, Xiujing Shen, Qian Zhao, Jianlin Bai, Minsheng You
Long non-coding RNAs (lncRNAs) are of particular interest because of their contributions to many biological processes. Here, we present the genome-wide identification and characterization of putative lncRNAs in a global insect pest, Plutella xylostella. A total of 8096 lncRNAs were identified and classified into three groups. The average length of exons in lncRNAs was longer than that in coding genes and the GC content was lower than that in mRNAs. Most lncRNAs were flanked by canonical splice sites, similar to mRNAs...
January 2018: Genomics
https://www.readbyqxmd.com/read/28769965/silicon-supplementation-alters-the-composition-of-herbivore-induced-plant-volatiles-and-enhances-attraction-of-parasitoids-to-infested-rice-plants
#10
Jian Liu, Jiwei Zhu, Pengjun Zhang, Liwei Han, Olivia L Reynolds, Rensen Zeng, Jinhong Wu, Yue Shao, Minsheng You, Geoff M Gurr
Silicon (Si) is important in plant defenses that operate in a direct manner against herbivores, and work in rice (Oryza sativa) has established that this is mediated by the jasmonate signaling pathway. Plant defenses also operate indirectly, by the production of herbivore induced plant volatiles (HIPVs) that attract predators and parasitoids of herbivores. These indirect defenses too are mediated by the jasmonate pathway but no earlier work has demonstrated an effect of Si on HIPVs. In this study, we tested the effect of Si supplementation versus Si deprivation to rice plants on subsequent HIPV production following feeding by the important pest, rice leaffolder (Cnaphalocrocis medinalis)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28683762/segmental-duplications-evolution-and-impact-among-the-current-lepidoptera-genomes
#11
Qian Zhao, Dongna Ma, Liette Vasseur, Minsheng You
BACKGROUND: Structural variation among genomes is now viewed to be as important as single nucleoid polymorphisms in influencing the phenotype and evolution of a species. Segmental duplication (SD) is defined as segments of DNA with homologous sequence. RESULTS: Here, we performed a systematic analysis of segmental duplications (SDs) among five lepidopteran reference genomes (Plutella xylostella, Danaus plexippus, Bombyx mori, Manduca sexta and Heliconius melpomene) to understand their potential impact on the evolution of these species...
July 6, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28632152/isolation-and-characterization-of-microsatellite-loci-for-cotesia-plutellae-hymenoptera-braconidae
#12
Tiansheng Liu, Fushi Ke, Shijun You, Wenbin Chen, Weiyi He, Minsheng You
Fourteen polymorphic microsatellite loci were isolated in this transcriptome-based data analysis for Cotesia plutellae, which is an important larval parasitoid of the worldwide pest Plutella xylostella. A subsequent test was performed for a wild C. plutellae population (N = 32) from Fuzhou, Fujian, southeastern China, to verify the effectiveness of the 14 microsatellite loci in future studies on C. plutellae genetic diversity. The observed number of alleles ranged from two to six. The expected and observed heterozygosity ranged from 0...
June 20, 2017: Insects
https://www.readbyqxmd.com/read/28501604/high-production-purification-biochemical-characterization-and-gene-analysis-of-a-novel-catalase-from-the-thermophilic-bacterium-ureibacillus-thermosphaericus-fzsf03
#13
Xianbo Jia, Xinjian Lin, Yandan Tian, Jichen Chen, Minsheng You
A catalase-producing thermophilic bacterium, Ureibacillus thermosphaericus FZSF03, was isolated from high-temperature compost. Catalase production in this strain increased 31 times and reached 57,630U/mL after optimization in a shake flask, which might represent the highest catalase activity level among reported wild strains. This catalase was further purified and identified. The purified enzyme showed a specific activity of 219,360U/mg, higher than many other catalases. The molecular weight of this enzyme is 52kDa according to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the enzyme was identified as a monofunctional haeme catalase of Ureibacillus thermosphaericus by liquid chromatography-mass spectrometry (LC-MS)/MS...
October 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28491055/metagenomic-sequencing-of-diamondback-moth-gut-microbiome-unveils-key-holobiont-adaptations-for-herbivory
#14
Xiaofeng Xia, Geoff M Gurr, Liette Vasseur, Dandan Zheng, Huanzi Zhong, Bingcai Qin, Junhan Lin, Yue Wang, FengQin Song, Yong Li, Hailan Lin, Minsheng You
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28422446/fusion-of-a-family-20-carbohydrate-binding-module-cbm20-with-cyclodextrin-glycosyltransferase-of-geobacillus-sp-chb1-improves-catalytic-efficiency
#15
Xianbo Jia, Yonghua Guo, Xinjian Lin, Minsheng You, Chenqiang Lin, Longjun Chen, Jichen Chen
Cyclodextrin glycosyltransferase (CGTase) is an important industrial enzyme for production of cyclodextrins (CDs) from starch by intramolecular transglycosylation. CGTase consists of five domains labeled A to E. For optimizing catalytic activity of CGTase, CGTase of Geobacillus sp. was fused with the family 20 carbohydrate-binding module (CBM) of the Bacillus circulans strain 251 CGTase. The CBMbc251 that has a low binding free energy with maltohexaose, was selected by in silico design. Then the fusion enzyme, CGTΔE-CBMbc251, was constructed by fusing the CBMbc251 to the C-terminal region of CGTΔE...
June 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28196471/characterization-and-expression-profiling-of-serine-protease-inhibitors-in-the-diamondback-moth-plutella-xylostella-lepidoptera-plutellidae
#16
Hailan Lin, Xijian Lin, Jiwei Zhu, Xiao-Qiang Yu, Xiaofeng Xia, Fengluan Yao, Guang Yang, Minsheng You
BACKGROUND: Serine protease inhibitors (SPIs) have been found in all living organisms and play significant roles in digestion, development and innate immunity. In this study, we present a genome-wide identification and expression profiling of SPI genes in the diamondback moth, Plutella xylostella (L.), a major pest of cruciferous crops with global distribution and broad resistance to different types of insecticides. RESULTS: A total of 61 potential SPI genes were identified in the P...
February 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28103237/adult-tea-green-leafhoppers-empoasca-onukii-matsuda-change-behaviors-under-varying-light-conditions
#17
Longqing Shi, Liette Vasseur, Huoshui Huang, Zhaohua Zeng, Guiping Hu, Xin Liu, Minsheng You
Insect behaviors are often influenced by light conditions including photoperiod, light intensity, and wavelength. Understanding pest insect responses to changing light conditions may help with developing alternative strategies for pest control. Little is known about the behavioral responses of leafhoppers (Hemiptera: Cicadellidae) to light conditions. The behavior of the tea green leafhopper, Empoasca onukii Matsuda, was examined when exposed to different light photoperiods or wavelengths. Observations included the frequency of locomotion and cleaning activities, and the duration of time spent searching...
2017: PloS One
https://www.readbyqxmd.com/read/27845388/avoidance-escape-and-microstructural-adaptations-of-the-tea-green-leafhopper-to-water-droplets
#18
Meizhen Lin, Liette Vasseur, Guang Yang, Geoff M Gurr, Minsheng You
Rain and dew droplets can dislodge or drown small insects and may be important factors that drive adaptations for avoidance and escape. Studying the microstructure of small insects and insect behaviour can help understand these adaptations. We quantified avoidance behaviour and entrapment of nymphs and adults of the tea green leafhopper (Empoasca onukii) using simulated rainfall onto host plant foliage and made observations of pretarsi and abdomen microstructures. Adults responded rapidly to simulated rainfall and escaped by jumping whilst most young nymphs were washed from water-sprayed leaves though older nymphs tended to remain on leaves and subsequently escaped from water droplets...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27833616/coconut-lethal-yellowing-diseases-a-phytoplasma-threat-to-palms-of-global-economic-and-social-significance
#19
REVIEW
Geoff M Gurr, Anne C Johnson, Gavin J Ash, Bree A L Wilson, Mark M Ero, Carmel A Pilotti, Charles F Dewhurst, Minsheng S You
The recent discovery of Bogia coconut syndrome in Papua New Guinea (PNG) is the first report of a lethal yellowing disease (LYD) in Oceania. Numerous outbreaks of LYDs of coconut have been recorded in the Caribbean and Africa since the late Nineteenth century and have caused the death of millions of palms across several continents during the Twentieth century. Despite the severity of economic losses, it was only in the 1970s that the causes of LYDs were identified as phytoplasmas, a group of insect-transmitted bacteria associated with diseases in many other economically important crop species...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27813664/habitat-management-to-suppress-pest-populations-progress-and-prospects
#20
REVIEW
Geoff M Gurr, Steve D Wratten, Douglas A Landis, Minsheng You
Habitat management involving manipulation of farmland vegetation can exert direct suppressive effects on pests and promote natural enemies. Advances in theory and practical techniques have allowed habitat management to become an important subdiscipline of pest management. Improved understanding of biodiversity-ecosystem function relationships means that researchers now have a firmer theoretical foundation on which to design habitat management strategies for pest suppression in agricultural systems, including landscape-scale effects...
January 31, 2017: Annual Review of Entomology
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