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Cheng Chen, Qiang Li, Qiangfeng Wang, Daihua Lu, Hong Zhang, Jian Wang, Rongtao Fu
Ganoderma is well known for its use in traditional Chinese medicine and is widely cultivated in China, Korea, and Japan. Increased temperatures associated with global warming are negatively influencing the growth and development of Ganoderma. Nitric oxide is reported to play an important role in alleviating fungal heat stress (HS). However, the transcriptional profiling of Ganoderma oregonense in response to HS, as well as the transcriptional response regulated by NO to cope with HS has not been reported. We used RNA-Seq technology to generate large-scale transcriptome data from G...
November 16, 2017: Scientific Reports
Nada K Sedky, Zaynab El Gammal, Amir E Wahba, Eman Mosad, Zahraa Y Waly, Amira Ali El-Fallal, Reem K Arafa, Nagwa El-Badri
OBJECTIVES: Despite advances in therapy of breast and ovarian cancers, they still remain among the most imperative causes of cancer death in women. The first can be considered one of the most widespread diseases among females, while the latter is more lethal and needs prompt treatment. Thus, the research field can still benefit from discovery of new compounds that can be of potential use in management of these grave illnesses. We hereby aimed to assess the antitumor activity of the phytosterol α-spinasterol isolated from Ganoderma resinaceum mushroom on human breast cancer cell lines (MCF-7, MDA-MB-231), as well as, on human ovarian cancer cell line (SKOV-3)...
November 16, 2017: Journal of Cellular Biochemistry
Didem Sohretoglu, Shile Huang
The mushroom Ganoderma lucidum (G. lucidum) has been used for centuries in Asian countries to treat various diseases and to promote health and longevity. Clinical studies have shown beneficial effects of G. lucidum as an alternative adjuvant therapy in cancer patients without obvious toxicity. G. lucidum polysaccharides (GLP) is the main bioactive component in the water soluble extracts of this mushroom. Evidence from in vitro and in vivo studies has demonstrated that GLP possesses potential anticancer activity through immunomodulatory, anti-proliferative, pro-apoptotic, anti-metastatic and anti-angiogenic effects...
November 13, 2017: Anti-cancer Agents in Medicinal Chemistry
Zhen Tan, Jin-Lian Zhao, Ji-Mei Liu, Min Zhang, Ri-Dao Chen, Ke-Bo Xie, Da-Wei Chen, Jun-Gui Dai
Two new lanostane triterpenoids (1 and 2), two new ergostane-type steroids (3 and 4) together with two known lanostane triterpenoids (5 and 6) and one known steroid (7) were isolated from the cultured mycelia of Ganoderma capense (CGMCC 5.71). Their structures were determined on the basis of extensive spectroscopic (HRESIMS, 1D NMR, 2D NMR) data analyses. Compound 1 exhibited moderate cytotoxic activity against the human cancer cell line NCI-H1650 with an IC50 value of 22.3 μM, and 7 displayed cytotoxic activity against the human cancer cell line HCT116 with an IC50 value of 17...
November 9, 2017: Journal of Asian Natural Products Research
Xiaohui Zhao, Dayu Zhou, Yunen Liu, Chun Li, Xiaoguang Zhao, Ying Li, Wei Li
Prostate cancer is one of the most common types of malignant tumor of men worldwide and the incidence and mortality rate is gradually increasing. At present, the molecular mechanisms of growth and migration in human prostate cancer have not been completely elucidated. Studies have demonstrated that Ganoderma lucidum polysaccharides (GLP) can inhibit cancer. Therefore the present study investigated the effect and molecular mechanism of GLP on cell growth and migration of LNCaP human prostate cancer cells. LNCaP cells were transfected with either a protein arginine methyltransferase 6 (PRMT6) overexpression plasmid or PRMT6 small interfering (si)RNA...
October 26, 2017: Molecular Medicine Reports
Titin Andri Wihastuti, Teuku Heriansyah
Background: Atherosclerosis occurs as a result of low-density lipoprotein (LDL) deposits oxidation. Endothelial dysfunction is an early process of atherosclerosis. Restoring endothelial lining back to normal by endothelial progenitor cells (EPCs) is critical for slowing or reversing vascular disease progression. Oxidative stress from hydrogen peroxide (H2O2) is increased in dyslipidemia so that antioxidant agent is required to prevent destruction of blood vessels. Objectives: This study aims to report Ganoderma lucidum polysaccharide peptide (PsP) effects in atherogenic process by measuring H2O2 level, IL-10 level, and EPC number in blood serum, and also intima-media thickness of aorta in dyslipidemia Wistar rat model by giving them a hypercholesterol diet (HCD)...
January 2017: Heart International
Guihua Xu, Jie Shen, Peng Sun, Yan Niu, Pengwei Zhao, Pingping Tang, Jiayi Zhang, Chunxue Fei, Leinan Bu, Zhiyi Yue, Honghao Liu, Zhiqiang Wang, Limin Yang, Dejun Sun
Although potato extract, derived from various methods, exhibits anticancer, antiviral and anti-parasite activities in vitro and in vivo, the bioactivity of potato solution remains unclear using the freeze-thaw extraction method granted by the State Intellectual Property Office of China. In the present study, a potato freeze-thaw solution (PFTS) was fed to mice with ascites tumor that were pre-treated with cyclophosphamide. The numbers of peripheral white blood cells (WBCs), macrophage phagocytosis, lymphocyte transformation and survival of mice were measured...
November 2017: Oncology Letters
Bulent Ergun
Ganoderma lucidum (GL) is a mushroom used as a traditional remedy for the treatment of various infections since ancient times. This study, was aimed to investigate antimicrobial activity potential of GL against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Candida glabrata, Candida krusei, and Candida parapsilopsis. Furthermore, it was also aimed to evaluate the toxicity potential of GL. Antimicrobial activities were screened by using microbroth dilution method...
September 2017: Pakistan Journal of Pharmaceutical Sciences
Theodorus Eko Pramudito, Delia Agustina, Thi Kim Ngan Nguyen, Antonius Suwanto
Bacterial antifungal cyclic lipopeptides (ACLs) have become a promising alternative to synthetic fungicide to control pathogenic fungi. Bacillus sp. is known to produce three families of ACL, namely iturin, surfactin, and fengycin. In this paper, we characterized the ACLs produced by B. methylotrophicus HC51 (referred as HC51) mainly regarding its composition and effectivity against fungal plant pathogen. HC51 culture was tested against various pathogenic fungi and the ACLs were extracted and analyzed using liquid chromatography-electrospray ionization mass spectrometry...
November 3, 2017: Probiotics and Antimicrobial Proteins
Xian-Qiang Che, Shao-Ping Li, Jing Zhao
A new triterpenoid and 18 analogues were isolated from the water extract of Ganoderma lucidum by column chromatographic techniques, including silica gel, ODS, Sephadex LH-20, and HPLC. The new compound was elucidated as 2β-acetoxy-3β,25-dihydroxy-7,11,15-trioxo-lanost-8-en-26-oic acid on the basis of analyses of extensive spectroscopic data and its physicochemical properties. Comparison of NMR data with those reported in literature, the known analogues were determined as ganoderic acid H (2), 12β-acetoxy-3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (3), ganoderenic acid D (4),ganoderic acid C1 (5),ganoderic acid G (6),3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid (7),ganoderic acid B (8),ganoderic acid C6 (9),3β,15α-dihydroxy-7,11,23-trioxo-lanost-8,16-dien-26-oic acid (10),ganoderic acid A (11),ganolucidic acid A (12),lucidenic acid E2 (13),lucidenic acid N (14),lucidenic acid P (15), lucidenic acid B (16),lucidenic acid A (17),lucidenic acid C (18),and lucidenic acid L (19), respectively...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Sittichai Sillapapongwarakorn, Somchai Yanarojana, Darawan Pinthong, Amnuay Thithapandha, Jiraporn Ungwitayatorn, Porntip Supavilai
Triterpenoids isolated from Ganoderma lucidum (GLTs) exhibit a broad spectrum of anti-cancer properties, including anti-proliferative, anti-metastatic and anti-angiogenic activities. Current research studies revealed the role by GLTs in inducing apoptosis and suppression of telomerase activity of cancer cells with much lower toxicity to healthy cells. Compounds selectively binding and stabilizing G-quadruplex structures could inhibit the telomerase or downregulate the oncogenes and may act as anti-cancer agents...
2017: Bioinformation
Balraj Singh Gill, Navgeet, Richa Mehra, Vicky Kumar, Sanjeev Kumar
Cancer is a multifactorial disease, causing behavioral and metabolic alterations, leading to uncontrolled cell proliferation with collateral weakening of immune system. Crucial balance between cell proliferation and cell death determines the fate of a cell, which might progress towards survival or apoptosis. Apoptosis is a complex, programmed, and highly regulated process causing dramatic morphological and biochemical perturbations in the cellular machinery. Ganoderma lucidum is a basidiomycetes, polypore mushroom known for its pharmacological properties in cancer...
October 28, 2017: Investigational New Drugs
Yifei Jiang, Yajing Chang, Yong Liu, Meng Zhang, Heng Luo, Cui Hao, Pengjiao Zeng, Yue Sun, Hua Wang, Lijuan Zhang
Ganoderma sinense or "Chinese Lingzhi" is a well-known medicinal fungus in China for more than 2000 years. Polysaccharide is the main immunomodulatory and antitumor component in G. sinense. In 2010, G. sinense polysaccharide (GSP) tablet is approved as an adjunctive therapeutic drug in China for treating leukopenia and hematopoietic injury caused by concurrent chemo/radiation therapy during cancer treatment by the State Food and Drug Administration (SFDA). β-glucan, an established immunostimulant, is one of the components in GSP...
October 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Yasushi Sukegawa, Junichiro Kawamura, Kiyotaka Okuno
Cyclophosphamide(CY)was intraperitoneally administered once a week to C57BL/10mice that had received Rous sarcoma virus(RSV)-induced S1018B10 syngeneic tumor transplantation and in whom tumor diameter exceeded 4.5 mm. Survival was prolonged in a group of mice that also received a mixture of LEM and MAK orally. When splenic cells were cultured under mitomycin C-treated S1018B10 stimulation and the S1018B10-directed cell killing ability was examined, the effector cells were found to be F4/80 - DC/Mф cells. Flow cytometric analysis showed that the proportion of F4/80- DC/Mф cells in the splenic cell culture of the CY+LEM+MAK treatment group was higher than that in the untreated group...
October 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
Rakrudee Sarnthima, Saranyu Khammaung, Piyawan Sa-Ard
Culture broth of Ganoderma lucidum was determined for antioxidant, antibacterial and α-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS(+·) and DPPH(·) radicals with a half maximal inhibitory concentration (IC50) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively and reducing power equaled to 4...
October 2017: Journal of Food Science and Technology
Tung Yuan Wang, Cheng-Chia Yu, Pei-Ling Hsieh, Yi-Wen Liao, Chuan-Hang Yu, Ming-Yung Chou
Cancer stem cells (CSCs) have been identified to exert tumor-initiating ability, resulting in the recurrence, metastasis and chemoresistance of oral squamous cell carcinomas. In the present study, we showed that GMI, an immunomodulatory protein from Ganoderma microsporum, induc ed a cytotoxic effect in oral carcinomas stem cells (OCSCs). Treatment of GMI dose-dependently inhibited the expression of CSC markers, including ALDH1 activity and CD44 positivity. Moreover, GMI suppressed the self-renewal property, colony formation, migration, and invasion abilities as well as potentiated chemo-sensitivity in OCSCs...
September 19, 2017: Oncotarget
Kang Li, Kun Na, Tingting Sang, Kaikai Wu, Ying Wang, Xingya Wang
The medicinal mushroom Ganoderma lucidum (G. lucidum) has been reported to possess a variety of pharmacological activities including anticancer effects. However, the anti-colorectal cancer effects and the potential molecular mechanisms of the ethanol extracts of sporoderm-broken spores of G. lucidum (BSGLEE), which mainly contains triterpenoids, have not been reported. The aim of the present study was to investigate the anticancer effects and molecular mechanisms exerted by BSGLEE on colorectal cancer in vitro and in vivo...
September 27, 2017: Oncology Reports
Wei Liu, Jingsong Zhang, Wei Han, Yanfang Liu, Jie Feng, Chuanhong Tang, Na Feng, Qingjiu Tang
Due to the difficulty and high cost for the preparation of triterpenes, one single standard for the simultaneous determination of multi-components (SSDMC) with high performance liquid chromatography (HPLC) is an advanced solution for multi-component analysis. Experiments were carried out to investigate the feasibility of SSDMC for the analysis of Ganoderma triterpenes, with external standard method (ESM) compared, and the samples of Ganoderma were classified by the content of Ganoderma triterpenes. The analysis was performed by using a Fortis Speed Core-C18 column (150mm×4...
October 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiaoyan Tan, Junshe Sun, Zhangyang Xu, Hengchang Li, Jing Hu, Huijuan Ning, Zifang Qin, Haisheng Pei, Tian Sun, Xiuqing Zhang
Ganoderma lucidum is a traditional Chinese medicine, and its polysaccharides possess diverse and significant pharmacological activities. This study aimed to investigate the polysaccharide production, molecular characteristics and in-vitro antioxidant activity of G. lucidum fruiting body after the mushroom was harvested and treated with heat stress (HS). HS enhanced the production of polysaccharides after harvest and treatment of 42 °C HS for 2 h, and that resulted in the highest polysaccharide yield of 10...
October 10, 2017: Bioprocess and Biosystems Engineering
Edwin D Morales-Álvarez, Claudia M Rivera-Hoyos, Sergio A Poveda-Cuevas, Edwin A Reyes-Guzmán, Aura M Pedroza-Rodríguez, Edgar A Reyes-Montaño, Raúl A Poutou-Piñales
The original version of this article unfortunately contained a mistake. The replacement image of Fig. 4 provided by the first corresponding author, Aura M. Pedroza-Rodríguez, is incorrect and that the originally submitted Fig. 4 should have been retained. The original article has been corrected.
October 9, 2017: Applied Biochemistry and Biotechnology
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