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https://www.readbyqxmd.com/read/29353449/blastulation-timing-is-associated-with-differential-mitochondrial-content-in-euploid-embryos
#1
Jacqueline R Ho, Nabil Arrach, Katherine Rhodes-Long, Wael Salem, Lynda K McGinnis, Karine Chung, Kristin A Bendikson, Richard J Paulson, Ali Ahmady
PURPOSE: Preimplantation genetic screening (PGS) and assessment of mitochondrial content (MC) are current methods for selection of the best embryos for transfer. Studies suggest that time-lapse morphokinetics (TLM) may also be helpful for selecting embryos more likely to implant. In our study, we sought to examine the relationship between TLM parameters and MC to determine if they could be used adjunctively in embryo selection. We also examined the relationship between MC with ploidy and blastulation...
January 20, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29353422/biomechanical-in-vitro-comparison-of-suture-anchors-for-thumb-ucl-repair
#2
M Wagner, W Schmoelz, H Stofferin, R Arora
Different types of intraosseous suture anchors can be used for thumb ulnar collateral ligament (UCL) repair surgery. Some of them have already been tested biomechanically in intact bone; however, there exists little knowledge of their stability when implanted in avulsion fracture. In this biomechanical in vitro study, three anchor types (hard, soft and novel BoneWelding) were tested on 24 fresh frozen human thumbs from 12 body donors. After the repair of an iatrogenic ligamentous UCL rupture, the thumbs were cyclically loaded...
January 20, 2018: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/29353316/validation-of-a-new-classification-for-periprosthetic-shoulder-fractures
#3
Chlodwig Kirchhoff, Marc Beirer, Ulrich Brunner, Arne Buchholz, Peter Biberthaler, Moritz Crönlein
INTRODUCTION: Successful treatment of periprosthetic shoulder fractures depends on the right strategy, starting with a well-structured classification of the fracture. Unfortunately, clinically relevant factors for treatment planning are missing in the pre-existing classifications. Therefore, the aim of the present study was to describe a new specific classification system for periprosthetic shoulder fractures including a structured treatment algorithm for this important fragility fracture issue...
January 20, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29353284/usefulness-of-totally-implantable-central-venous-access-devices-in-elderly-patients-a-retrospective-study
#4
Yuki Imaoka, Fumito Kuranishi, Yoshiteru Ogawa
BACKGROUND/AIMS: The need for totally implantable central venous access devices (TICVADs) has increased with increased opportunities in the use of chemotherapy and parenteral nutrition. This study aimed to determine the outcomes of TICVAD implantation and use in patients aged ≥85 years. METHODS: Between January 2010 and August 2016, 117 patients underwent TICVAD implantation and their records were retrospectively reviewed. RESULTS: Participants were divided into 2 groups (plus-85 and sub-85 groups)...
January 19, 2018: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/29353144/effects-of-the-hdac-inhibitor-scriptaid-on-the-in%C3%A2-vitro-development-of-bovine-embryos-and-on-imprinting-gene-expression-levels
#5
R Laguna-Barraza, M J Sánchez-Calabuig, A Gutiérrez-Adán, D Rizos, S Pérez-Cerezales
This study examines the effects of the histone deacetylation inhibitor scriptaid (SCR) on preimplantation embryo development in vitro and on imprinting gene expression. We hypothesized that SCR would increase histone acetylation levels, enhance embryonic genome activation, and regulate imprinting and X-chromosome inactivation (XCI) in in vitro produced bovine embryos. Zygotes were cultured in vitro in presence or absence of SCR added at different time points. We assessed cleavage and blastocyst rates as well as the quality of blastocysts through: (i) differential cell counts; (ii) survival after vitrification/thawing and (iii) gene expression analysis -including imprinted genes...
January 11, 2018: Theriogenology
https://www.readbyqxmd.com/read/29353106/perineurium-like-sheath-derived-from-long-term-surviving-mesenchymal-stem-cells-confers-nerve-protection-to-the-injured-spinal-cord
#6
Yuan-Huan Ma, Xiang Zeng, Xue-Cheng Qiu, Qing-Shuai Wei, Ming-Tian Che, Ying Ding, Zhou Liu, Guo-Hui Wu, Jia-Hui Sun, Mao Pang, Li-Min Rong, Bin Liu, Zaid Aljuboori, Inbo Han, Eng-Ang Ling, Yuan-Shan Zeng
The functional multipotency enables mesenchymal stem cells (MSCs) promising translational potentials in treating spinal cord injury (SCI). Yet the fate of MSCs grafted into the injured spinal cord has not been fully elucidated even in preclinical studies, rendering concerns of their safety and genuine efficacy. Here we used a rat spinal cord transection model to evaluate the cell fate of allograft bone marrow derived MSCs. With the application of immunosuppressant, donor cells, delivered by biocompatible scaffold, survived up to 8 weeks post-grafting...
January 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29352934/%C3%AE-cyclodextrin-phenylacetic-acid-mesh-as-a-drug-trap
#7
Hyeong Sup Yu, Jae Min Lee, Yu Seok Youn, Kyung Taek Oh, Kun Na, Eun Seong Lee
In this study, we developed a nanoporous biodegradable mesh, bioinspired by the spider web, which is prepared via electrospinning using γ-cyclodextrin (γ-CD) conjugated with phenylacetic acid (PA), named γ-CDP. The resulting γ-CDP has a microfibrous or microspherical shape and contains drug trap meshlike γ-CD pores. These γ-CDP micromeshes (microspheres or microfibers) enable efficient drug capture and drug transport into deep γ-CDP nanocompartments or out of the γ-CDP web, resulting in a driving domain for a 4-week drug release...
March 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29352772/neurotransmitters-and-receptors-changes-in-medial-nucleus-of-the-trapezoid-body-mntb-of-early-developmental-rats-with-single-side-deafness
#8
Jinsheng Dai, Jinfeng Liu, Mo Zhou, Wenjiao Wang, Zhi-Qing David Xu, Ningyu Wang
BACKGROUND Congenital single-side deafness (SSD) affects sound localization even after cochlear implantation (CI) in some conditions. The medial nucleus of the trapezoid body (MNTB) plays an important role in binaural benefit and sound localization, but little is known about intrinsic molecular changes in MNTB with SSD. We aimed to observe changes in MNTB in early-developmental SSD rats, including the key neurotransmitters (GABA, Gly, Glu) and major receptors (GABAa-R/GABAb-R for GABA, Gly-R for Gly, and AMPA/NMDA for Glu)...
January 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29352728/differential-proteomic-analysis-of-synovial-fluid-from-hip-arthroplasty-patients-with-a-pseudotumor-vs-periprosthetic-osteolysis
#9
Isabelle Catelas, Eric A Lehoux, Zhibin Ning, Daniel Figeys, Stephen J Baskey, Paul E Beaulé
Adverse tissue reactions to metal implants, including pseudotumors, can compromise implant functionality and survivorship. The identification of specific proteins in the synovial fluid (SF) of hip arthroplasty patients with a pseudotumor may lead to a better understanding of the underlying pathomechanisms. The objective of the present study was to compare the protein content of SF from patients with a short-term metal-on-metal hip implant associated with a pseudotumor and patients with a long-term metal-on-polyethylene hip implant associated with periprosthetic osteolysis...
January 20, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29352701/bailout-surgical-explantation-of-a-transcatheter-valve-in-valve-for-subacute-thrombosis-when-there-is-no-time-for-anticoagulation-case-report-and-literature-review
#10
Francesco Notaristefano, Matteo Rocco Reccia, Salvatore Notaristefano, Roberto Annunziata, Rocco Sclafani, Giuseppe Ambrosio, Claudio Cavallini
Trancatheter heart valve (THV) thrombosis is effectively treated with anticoagulation but alternative therapies are required when a faster gradient reduction is needed. Open heart surgery has been rarely performed due to the high perioperative mortality and only five cases have been described so far. Here we describe a case of emergent surgical explantation for THV thrombosis after a valve-in-valve. A 67years old man underwent transcatheter aortic valve implantation for a failed surgical bioprosthesis Epic 21mm (St...
December 13, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29352699/computed-tomography-ct-assessment-of-the-membranous-septal-anatomy-prior-to-transcatheter-aortic-valve-replacement-tavr-with-the-balloon-expandable-sapien-3-valve
#11
Brett A Oestreich, Mackenzie Mbai, Sergey Gurevich, Prabhjot S Nijjar, Selcuk Adabag, Stefan Bertog, Rosemary Kelly, Santiago Garcia
OBJECTIVES: The lower limit of the membranous septum (MS) is considered an anatomic landmark for the emergence of the Bundle of His into the left ventricle. Computed tomography (CT) assessment of MS anatomy may provide useful information about the risk of conduction abnormalities following transcatheter aortic valve replacement (TAVR). METHODS AND RESULTS: The study included 102 consecutive patients undergoing TAVR with the Edwards Sapien 3 (S3) valve. Using pre-TAVR CT and post-procedure angiography we evaluated for the presence of calcium in the left ventricular outflow tract (LVOT), calcium depth (CD), implantation depth (ID) and MS length...
December 27, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29352690/diagnostic-utility-of-histological-analysis-for-detecting-ongoing-infection-during-two-stage-revision-arthroplasty-in-patients-with-inflammatory-arthritis
#12
Jaiben George, Yaxia Zhang, Michael Jawad, Mhamad Faour, Alison K Klika, Thomas W Bauer, Carlos A Higuera
BACKGROUND: Surgeons often rely on intra-operative histology (frozen sections [FS]) to determine the next step in surgical management during the second stage (re-implantation surgery) of 2-stage revision arthroplasty. The purpose of the study is to assess the accuracy of permanent sections (PS) and FS in the diagnosis of persistent infection during re-implantation in patients with an inflammatory arthritis. METHODS: From 2001 to 2016, 47 planned second-stage revision total hip arthroplasty and total knee arthroplasty in patients with inflammatory arthritis were identified...
December 29, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29352684/long-term-outcome-of-hip-arthroplasty-in-the-setting-of-pigmented-villonodular-synovitis
#13
Meagan E Tibbo, Cody C Wyles, Peter S Rose, Franklin H Sim, Matthew T Houdek, Michael J Taunton
BACKGROUND: Pigmented villonodular synovitis (PVNS) is a rare, benign, painful proliferation of the synovium previously treated successfully with total hip arthroplasty (THA). Published results come from small series; therefore, the purpose of this study is to investigate the outcomes of THA in the setting of PVNS. METHODS: We identified 25 patients with histologically confirmed, diffuse PVNS who underwent THA between 1971 and 2013. Mean follow-up and age was 10 years and 39 years...
December 29, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29352681/no-consensus-for-femoral-head-impaction-technique-in-surgeon-education-materials-from-orthopedic-implant-manufacturers
#14
Brian J McGrory, Eric Ng
BACKGROUND: Prior studies indicate that one factor that may contribute to total hip arthroplasty failure due to mechanically assisted crevice corrosion at the femoral head-trunnion junction is the method of femoral head fixation. METHODS: Up-to-date on-line surgical technique guides describing fixation of the prosthetic femoral head of common implants of the 4 largest manufacturers as well as 2 minor manufacturers were identified. The information given regarding preparation and fixation of the femoral head was evaluated and compared...
December 30, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29352637/the-ability-of-topical-and-systemic-statins-to-increase-osteogenesis-around-dental-implants-a-systematic-review-of-histomorphometric-outcomes-in-animal-studies
#15
REVIEW
V Moraschini, D C F Almeida, J A Calasans-Maia, M Diuana Calasans-Maia
The purpose of this systematic review was to evaluate the quantitative histomorphometric outcomes of animal studies investigating statins as a pro-osteogenic agent to enhance the osseointegration of dental implants. Some animal studies have suggested a beneficial action of statins on bone tissue. Electronic and manual literature searches, without date or language restriction, ​​were performed by two independent review authors up to February 2017. Eligibility criteria included animal trials quantitatively analysing the pro-osteogenic effect of statins on dental implants...
January 15, 2018: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29352461/clinical-and-microbiological-evaluation-of-the-effect-of-lactobacillus-reuteri-in-the-treatment-of-mucositis-and-peri-implantitis-a-triple-blind-randomized-clinical-trial
#16
M Galofré, D Palao, M Vicario, J Nart, D Violant
BACKGROUND AND OBJECTIVE: Oral probiotics appear to improve the treatment of periodontal diseases but there is limited evidence on their efficacy in the treatment of peri-implant diseases. The objective of the present study was to evaluate, clinically and microbiologically, the effect of the oral probiotic, Lactobacillus reuteri Prodentis, as adjuvant to non-surgical mechanical therapy in implants with mucositis or peri-implantitis, placed in patients with a history of periodontal disease...
January 19, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29352347/autologous-cell-based-therapy-for-treatment-of-large-bone-defects-from-bench-to-bedside
#17
REVIEW
R Verboket, M Leiblein, C Seebach, C Nau, M Janko, M Bellen, H Bönig, D Henrich, I Marzi
OBJECTIVES: Reconstruction of long segmental bone defects is demanding for patients and surgeons, and associated with long-term treatment periods and substantial complication rates in addition to high costs. While defects up to 4-5 cm length might be filled up with autologous bone graft, heterologous bone from cadavers, or artificial bone graft substitutes, current options to reconstruct bone defects greater than 5 cm consist of either vascularized free bone transfers, the Masquelet technique or the Ilizarov distraction osteogenesis...
January 19, 2018: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/29352303/perfusion-decellularization-of-a-human-limb-a-novel-platform-for-composite-tissue-engineering-and-reconstructive-surgery
#18
Mattia Francesco Maria Gerli, Jacques Paul Guyette, Daniele Evangelista-Leite, Brian Burns Ghoshhajra, Harald Christian Ott
Muscle and fasciocutaneous flaps taken from autologous donor sites are currently the most utilized approach for trauma repair, accounting annually for 4.5 million procedures in the US alone. However, the donor tissue size is limited and the complications related to these surgical techniques lead to morbidities, often involving the donor sites. Alternatively, recent reports indicated that extracellular matrix (ECM) scaffolds boost the regenerative potential of the injured site, as shown in a small cohort of volumetric muscle loss patients...
2018: PloS One
https://www.readbyqxmd.com/read/29352218/nutritional-deficiencies-and-bone-metabolism-after-endobarrier-in-obese-type-2-patients-with-diabetes
#19
Nuria Vilarrasa, Alexandra Fabregat, Silvia Toro, Amador García Gordejuela, Anna Casajoana, Mónica Montserrat, Pilar Garrido, Rafael López-Urdiales, Núria Virgili, Alejandra Planas-Vilaseca, Andreu Simó-Servat, Jordi Pujol
Endobarrier® is a minimally invasive, reversible endoscopic treatment for obesity. It provokes malabsorption along 60 cm of the small intestine, which can contribute to the development of vitamin deficiencies and to changes in bone mineral density (BMD). To determine the prevalence of nutrient deficiencies, changes in body composition and BMD during the first year after Endobarrier® placement. Twenty-one patients with type 2 diabetes met inclusion criteria. Levels of vitamins, micro and macronutrients were assessed prior and at 1, 3 and 12 months post-operatively...
January 19, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29352147/pre-conception-maternal-erythrocyte-saturated-to-unsaturated-fatty-acid-ratio-predicts-pregnancy-after-natural-cycle-frozen-embryo-transfer
#20
Christopher C Onyiaodike, Heather M Murray, Ruiqi Zhang, Barbara J Meyer, Fiona Jordan, E Ann Brown, Robert J B Nibbs, Helen Lyall, Naveed Sattar, Scott M Nelson, Dilys J Freeman
The environment for embryo implantation and fetal growth and development is affected by maternal nutritional, metabolic and health status. The aim of this prospective, cohort study was to test whether plasma metabolic and inflammatory biomarkers can predict pregnancy resulting from in vitro fertilisation (IVF). Women with a natural menstrual cycle undergoing frozen embryo transfer (FET) were recruited and fasting baseline blood samples were collected a mean of 3.4 days prior to the luteinising hormone (LH) surge and a non-fasting blood sample was taken on the day of FET...
January 19, 2018: Scientific Reports
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