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https://www.readbyqxmd.com/read/28432753/identification-of-indothiazinone-as-a-natural-antiplatelet-agent
#1
Chansik Yang, Sugyeong Kwon, Se-Jong Kim, Minseon Jeong, Ji-Young Park, Dongeun Park, Soon Jun Hong, Jong-Wha Jung, Chungho Kim
Cardiovascular disease, which is caused by unregulated platelet aggregation, is one of the main causes of deaths worldwide. Many studies have focused on natural products with antiplatelet effects as a safe alternative therapy in order to prevent the disease. In this context, an in-house chemical library was screened to find natural products capable of inhibiting the interaction between platelet integrin αIIbβ3 and fibrinogen, which is an essential step in platelet aggregation. On the basis of the screening results, indothiazinone, an alkaloid found in microbial cultures, was identified as a potential antiplatelet agent...
April 22, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28421694/molecular-engineering-and-plant-expression-of-an-immunoglobulin-heavy-chain-scaffold-for-delivery-of-a-dengue-vaccine-candidate
#2
Mi-Young Kim, Craig Van Dolleweerd, Alastair Copland, Matthew John Paul, Sven Hofmann, Gina R Webster, Emily Julik, Ivonne Ceballos-Olvera, Jorge Reyes-Del Valle, Moon-Sik Yang, Yong-Suk Jang, Rajko Reljic, Julian K Ma
In order to enhance vaccine uptake by the immune cells in vivo, molecular engineering approach was employed to construct a Polymeric Immunoglobulin G Scaffold (PIGS) that incorporates multiple copies of an antigen and targets the Fc gamma receptors on antigen-presenting cells. These self-adjuvanting immunogens were tested in the context of dengue infection, for which there is currently no globally licensed vaccine yet. Thus, the consensus domain III sequence (cEDIII) of dengue glycoprotein E was incorporated into PIGS and expressed in both tobacco plants and Chinese Ovary Hamster cells...
April 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28412866/comparison-of-the-morphokinetic-parameters-of-embryos-according-to-ovarian-reserve-in-ivf-cycles
#3
Suleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Hayriye Celenk, Ferda Burcu Tamer, Selcuk Erkilinc
The aim of this study is to evaluate the impact of ovarian reserve and age of women on early morphokinetic parameters of embryos with a time-lapse monitoring system. In total, 197 infertile couples with poor ovarian reserve (Group 1, n = 41), normal ovarian reserve (Group 2, n = 59), or polycystic ovaries (Group 3, n = 97) were included. The time from insemination to the following events were analyzed: pronuclear fading (Pnf) and cleavage to 2, 3, 4 and 5 cells. The optimal ranges for morphokinetic parameters of t5, s2 and cc2 in each group were also evaluated...
April 17, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28412857/prevalence-of-diminished-ovarian-reserve-in-chinese-women-with-polycystic-ovary-syndrome-and-sensitive-diagnostic-parameters
#4
Jing Jin, Xiangyan Ruan, Lin Hua, Xuanxuan Tian, Yanglu Li, Lijuan Wang, Alfred O Mueck
OBJECTIVES: To assess the prevalence of diminished ovarian reserve (DOR) in patients with polycystic ovary syndrome (PCOS) in China, and to search for sensitive diagnostic parameters. METHODS: Three hundred and thirty eight PCOS women aged 20-39 years were recruited. Basic characteristics, follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone and anti-Müllerian hormone (AMH) were measured. The best indices to diagnose PCOS with DOR were assessed...
April 17, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28409838/modulation-and-modeling-of-monoclonal-antibody-n-linked-glycosylation-in-mammalian-cell-perfusion-reactors
#5
Daniel J Karst, Ernesto Scibona, Elisa Serra, Jean-Marc Bielser, Jonathan Souquet, Matthieu Stettler, Hervé Broly, Miroslav Soos, Massimo Morbidelli, Thomas K Villiger
Mammalian cell perfusion cultures are gaining renewed interest as an alternative to traditional fed-batch processes for the production of therapeutic proteins, such as monoclonal antibodies (mAb). The steady state operation at high viable cell density allows the continuous delivery of antibody product with increased space-time yield and reduced in-process variability of critical product quality attributes (CQA). In particular, the production of a confined mAb N-linked glycosylation pattern has the potential to increase therapeutic efficacy and bioactivity...
April 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28409824/a-comparative-genomic-hybridization-approach-to-study-gene-copy-number-variations-among-chinese-hamster-cell-lines
#6
Nandita Vishwanathan, Arpan Bandyopadhyay, Hsu-Yuan Fu, Kathryn C Johnson, Nathan M Springer, Wei-Shou Hu
Chinese Hamster Ovary (CHO) cells are aneuploid in nature. The genome of recombinant protein producing CHO cell lines continuously undergoes changes in its structure and organization. We analyzed nine cell lines, including parental cell lines, using a comparative genomic hybridization (CGH) array focused on gene-containing regions. The comparison of CGH with copy-number estimates from sequencing data showed good correlation. Hierarchical clustering of the gene copy number variation data from CGH data revealed the lineage relationships between the cell lines...
April 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28409820/identification-and-control-of-novel-growth-inhibitors-in-fed-batch-cultures-of-chinese-hamster-ovary-cells
#7
Bhanu Chandra Mulukutla, Jaitashree Kale, Taylor Kalomeris, Michaela Jacobs, Gregory W Hiller
Chinese hamster ovary (CHO) cells in culture are known to consume large amounts of nutrients and divert most of them towards byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. Glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yielding higher peak cell densities and titers. Interestingly, even in such optimized cultures, cell growth slows and eventually stops, indicating the emergence of other factors that negatively affect cell growth...
April 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28401606/transcriptional-polymorphism-of-pirna-regulatory-genes-underlies-the-mariner-activity-in-d-simulans-testes
#8
Bastien Saint-Leandre, Isabelle Clavereau, Aurelie Hua-Van, Pierre Capy
During colonization of new areas, natural populations have to deal with changing environments, and transposable elements (TEs) can be useful "tools" in the adaptation process since they are major contributor to the structural and functional evolution of genomes. In this general context, the activity (copy number, transcriptional and excision rate) of the mariner mos1 element was estimated in 19 natural populations of D. simulans. It is shown (1) that mos1 expression is always higher and more variable in testes than in ovaries; (2) that mos1 activity is higher in colonizing populations compared to the sub-Saharan African ones (ancestral populations); (3) that mos1 variations in transcript levels and copy number are negatively correlated to transcriptional variations of piRNA genes, aubergine and argonaute3...
April 12, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28390152/reproductive-morphology-of-oarfish-regalecus-russellii
#9
Kristy L Forsgren, Homam Jamal, Andrew Barrios, E W Misty Paig-Tran
Reproduction is a critical aspect of understanding the biology of fishes. Relatively little is known about oarfish (Regalecus russellii) reproduction, however strandings of dead animals have provided a rare opportunity to investigate the gonadal morphology of four fish; two females and two males. A female collected in June 2015 (4.32 m TL) had bifurcated ovaries 2.14 m in length and 2.14 kg. The gonadosomatic index (GSI) was 11.8% and the fish was determined to be spawning capable/spawning reproductive phase...
April 8, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28390103/asn1-encoded-asparagine-synthetase-in-floral-organs-contributes-to-nitrogen-filling-in-arabidopsis-seeds
#10
Laure Gaufichon, Anne Marmagne, Katia Belcram, Tadakatsu Yoneyama, Yukiko Sakakibara, Toshiharu Hase, Olivier Grandjean, Clément Gilles, Sylvie Citerne, Stéphanie Boutet-Mercey, Céline Masclaux-Daubresse, Fabien Chardon, Fabienne Soulay, Xiaole Xu, Marion Trassaert, Maryam Shakiebaei, Amina Najihi, Akira Suzuki
Despite a general view that asparagine synthetase generates asparagine as a long-distance nitrogen transport amino acid to sink organs, its role in nitrogen metabolic pathways in floral organs during seed nitrogen filling has remained undefined. We demonstrate that onset of pollination in Arabidopsis induces selected genes of asparagine metabolism particularly at ovule stage (stage 0): ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), which accompanies enhanced asparagine synthetase protein, asparagine and total amino acids...
April 8, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28389520/oocyte-stem-cells-fact-or-fantasy
#11
Corrina J Horan, Suzannah Alice Williams
For many decades, the dogma prevailed that female mammals had a finite pool of oocytes at birth and this was gradually exhausted during a lifetime of reproductive function. However, in 2004, a new era began in the field of female oogenesis. A study was published that appeared to detect oocyte-stem cells capable of generating new eggs within mouse ovaries. This study was highly controversial and the years since this initial finding have produced extensive research and even more extensive debate into their possibility...
April 7, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28379636/conserved-insulin-signaling-in-the-regulation-of-oocyte-growth-development-and-maturation
#12
REVIEW
Debabrata Das, Swathi Arur
Insulin signaling regulates various aspects of physiology, such as glucose homeostasis and aging, and is a key determinant of female reproduction in metazoans. That insulin signaling is crucial for female reproductive health is clear from clinical data linking hyperinsulinemic and hypoinsulinemic condition with certain types of ovarian dysfunction, such as altered steroidogenesis, polycystic ovary syndrome, and infertility. Thus, understanding the signaling mechanisms that underlie the control of insulin-mediated ovarian development is important for the accurate diagnosis of and intervention for female infertility...
April 5, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28374493/expression-of-juvenile-hormone-acid-o-methyltransferase-and-juvenile-hormone-synthesis-in-blattella-germanica
#13
Claudia V Dominguez, Jose L Maestro
Juvenile hormone (JH), a sesquiterpenoid synthetized by the insect corpora allata (CA), plays critical roles in metamorphosis and reproduction. Penultimate or last step of JH synthesis is catalyzed by juvenile hormone acid O-methyltransferase (JHAMT). Here we report the cloning and expression analysis of the JHAMT orthologue in the cockroach, Blattella germanica (L.) (BgJHAMT). BgJHAMT is mainly expressed in CA, with only expression traces in ovary. Three different isoforms, differing in the 3'-UTR sequence, were identified...
April 4, 2017: Insect Science
https://www.readbyqxmd.com/read/28371547/mir-143-targets-mapk7-in-cho-cells-and-induces-a-hyperproductive-phenotype-to-enhance-production-of-difficult-to-express-proteins
#14
Melanie Schoellhorn, Simon Fischer, Andreas Wagner, René Handrick, Kerstin Otte
In recent years, the number of complex but clinically effective biologicals such as multi-specific antibody formats and fusion proteins has increased dramatically. However, compared to classical monoclonal antibodies (mAbs), these rather artificially designed therapeutic proteins have never undergone millions of years of evolution and thus often turn out to be difficult-to-express using mammalian expression systems such as Chinese hamster ovary (CHO) cells. In order to provide access to these sophisticated but effective drugs, host cell engineering of CHO production cell lines represents a promising approach to overcome low production yields...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28371489/a-strategy-to-accelerate-protein-production-from-a-pool-of-clones-in-chinese-hamster-ovary-cells-for-toxicology-studies
#15
Zhilan Hu, Wendy Hsu, Abby Pynn, Domingos Ng, Donna Quicho, Yilma Adem, Zephie Kwong, Brad Mauger, John Joly, Bradley Snedecor, Michael W Laird, Dana C Andersen, Amy Shen
In the biopharmaceutical industry, a clonally derived cell line is typically used to generate material for Investigational New Drug (IND)-enabling toxicology studies. The same cell line is then used to generate material for clinical studies. If a pool of clones can be used to produce material for IND-enabling toxicology studies ("Pool for Tox strategy") during the time a lead clone is being selected for clinical material production, the toxicology studies can be accelerated significantly (approximately four months at Genentech), leading to a potential acceleration of four months for the IND submission...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28371311/low-glucose-concentrations-within-typical-industrial-operating-conditions-have-minimal-effect-on-the-transcriptome-of-recombinant-cho-cells
#16
Yogender Kumar Gowtham, Christopher A Saski, Sarah W Harcum
Typically, mammalian cell culture medium contains high glucose concentrations that are analogous to diabetic levels in humans, suggesting that mammalian cells are cultivated in excessive glucose. Using RNA-Seq, this study characterized the Chinese hamster ovary (CHO) cell transcriptome under two glucose concentrations to assess the genetic effects associated with metabolic pathways, in addition to other global responses. The initial extracellular glucose concentrations used represented high (30 mM) and low (10 mM) glucose conditions, where at the time the transcriptomes were compared, the glucose concentrations were approximately 24 and 4...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28370206/adjuvant-psychological-therapy-in-long-term-endocrine-conditions
#17
J Daniels, J M Turner-Cobb
Consideration of psychological distress in long term endocrine conditions is of vital importance given the prevalence of anxiety and depression in such disorders. Poor mental health can lead to compromised self-care, higher utilisation of health services, lower rates of adherence, reduced quality of life and ultimately poorer outcomes. Adjuvant psychological therapy offers an effective resource to reduce distress in endocrinological disorders. While the vast majority of work in this area has focused on psychological screening and intervention in diabetes, identification and recognition of psychological distress is equally important in other endocrinological conditions, with supportive evidence in Polycystic Ovary Syndrome and Addison's disease...
March 30, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28369727/n-terminal-or-signal-peptide-sequence-engineering-prevents-truncation-of-human-monoclonal-antibody-light-chains
#18
S J Gibson, N J Bond, S Milne, A Lewis, A Sheriff, G Pettman, R Pradhan, D R Higazi, D Hatton
Monoclonal antibodies (mAbs) contain short N-terminal signal peptides on each individual polypeptide that comprises the mature antibody, targeting them for export from the cell in which they are produced. The signal peptide is cleaved from each heavy chain (Hc) and light chain (Lc) polypeptide after translocation to the ER and prior to secretion. This process is generally highly efficient, producing a high proportion of correctly cleaved Hc and Lc polypeptides. However, mis-cleavage of the signal peptide can occur, resulting in truncation or elongation at the N-terminus of the Hc or Lc...
March 28, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28361557/protective-effect-of-alpha-lipoic-acid-in-methotrexate-induced-ovarian-oxidative-injury-and-decreased-ovarian-reserve-in-rats
#19
Oya Soylu Karapinar, Neslihan Pinar, Oğuzhan Özcan, Tümay Özgür, Kenan Dolapçıoğlu
To determine whether the possible oxidative effect of methotrexate (Mtx) on ovary and to evaluate the effectiveness of alpha lipoic acid (ALA), which may be useful in many oxidative stress models. Thirty-two female Wistar-albino rats were randomly divided into four groups; control group, alpha lipoic acid group (ALA 100 mg/kg, 10 days), multiple dose Mtx group (Mtx 1 mg/kg 1, 3, 5, 7 days) and Mtx and ALA group (Mtx 1 mg/kg 1, 3, 5, 7 days and ALA 100 mg/kg, 10 days). Serum total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI), tumor necrosis factor-alpha (TNF-α), tissue malondialdehyde (MDA) and activities of glutathione peroxidase (GSH-Px) and catalase (CAT) and anti-Mullerian hormone (AMH) and total ovarian follicle count were evaluated...
March 31, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28345252/amplification-of-fsh-signaling-by-cftr-and-nuclear-soluble-adenylyl-cyclase-in-the-ovary
#20
Hui Chen, Hsiao Chang Chan
cAMP/PKA pathway is one of the most important signaling pathways widely distributed in most eukaryotic cells. The activation of the canonical cAMP/PKA pathway depends on transmembrane adenylyl cyclase (tmAC). Recently, soluble adenylyl cyclase (sAC), which is activated by HCO3(-) or Ca(2+) , emerges to provide an alternative way to activate cAMP/PKA pathway with the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated Cl(-) / HCO3(-) -conducting anion channel, as a key player. This review summarizes new progress in the investigation of the CFTR/HCO3(-) -dependent sAC signaling and its essential role in various reproductive processes, particularly in ovarian functions...
March 27, 2017: Clinical and Experimental Pharmacology & Physiology
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