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https://www.readbyqxmd.com/read/29682888/a-polymeric-immunoglobulin-antigen-fusion-protein-strategy-for-enhancing-vaccine-immunogenicity
#1
Gina R Webster, Craig van Dolleweerd, Thais Guerra, Szymon Stelter, Sven Hofmann, Mi-Young Kim, Audrey Y-H Teh, Gil Reynolds Diogo, Alastair Copland, Mathew Paul, Peter Hart, Rajko Reljic, Julian K-C Ma
In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS), was used to produce a vaccine candidate for Mycobacterium tuberculosis. A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered. Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana. The recombinant protein assembled into polymeric structures (TB-PIGS) in N. benthamiana, similar in size to polymeric IgM...
April 23, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29667174/impact-of-prior-cancer-history-on-the-overall-survival-of-patients-newly-diagnosed-with-cancer-a-pan-cancer-analysis-of-the-seer-database
#2
Huaqiang Zhou, Yan Huang, Zeting Qiu, Hongyun Zhao, Wenfeng Fang, Yunpeng Yang, Yuanyuan Zhao, Xue Hou, Yuxiang Ma, Shaodong Hong, Ting Zhou, Yaxiong Zhang, Li Zhang
The population of cancer survivors with prior cancer is rapidly growing. Whether a prior cancer diagnosis interferes with outcome is unknown. We conducted a pan-cancer analysis to determine the impact of prior cancer history for patients newly diagnosed with cancer. We identified 20 types of primary solid tumors between 2004 to 2008 in the Surveillance, Epidemiology, and End Results (SEER) database. Demographic and clinicopathologic variables were compared by chi-squared test and t-test as appropriate. The propensity score-adjusted Kaplan-Meier method and Cox proportional hazards models were used to evaluate the impact of prior cancer on overall survival (OS)...
April 18, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29665036/stable-mirna-overexpression-in-human-cap-cells-engineering-alternative-production-systems-for-advanced-manufacturing-of-biologics-using-mir-136-and-mir-3074
#3
Benjamin L Weis, Nadine Guth, Simon Fischer, Silke Wissing, Simon Fradin, Karl-Heinz Holzmann, René Handrick, Kerstin Otte
Chinese hamster ovary (CHO) cells still represent the major production host for therapeutic proteins. However, multiple limitations have been acknowledged leading to the search for alternative expression systems. CEVEC's amniocyte production (CAP) cells are human production cells demonstrated to enable efficient overexpression of recombinant proteins with human glycosylation pattern. However, CAP cells have not yet undergone any engineering approaches to optimize process parameters for a cheaper and more sustainable production of biopharmaceuticals...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29664789/what-is-new-in-the-management-of-poor-ovarian-response-in-ivf
#4
Alberto Vaiarelli, Danilo Cimadomo, Nicolò Ubaldi, Laura Rienzi, Filippo Maria Ubaldi
PURPOSE OF REVIEW: The management and treatment of patients with poor ovarian response is still a controversial issue in IVF. Increasing evidences demonstrate that the number of oocytes retrieved after a controlled ovarian stimulation (COS) greatly influences the clinical outcome in terms of cumulative live birth per started cycle. For this reason, any COS should aim to optimize the number of oocytes according to the ovarian reserve of the patient. The aim of this review is to provide an overview of new strategies proposed to manage poor responders according to the novel POSEIDON classification...
April 16, 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29662617/premature-ovarian-aging-in-brca-carriers-a-prototype-of-systemic-precocious-aging
#5
Irit Ben-Aharon, Mattan Levi, David Margel, Rinat Yerushalmi, Shulamith Rizel, Shlomit Perry, Eran Sharon, Noa Hasky, Ronit Abir, Benny Fisch, Ana Tobar, Ruth Shalgi, Salomon Marcello Stemmer
Purpose: Though former evidence implies a correlation of breast cancer susceptibility gene ( BRCA ) mutation with reduced ovarian reserve, the data is yet inconsistent. Our aim was to investigate biomarkers of ovarian aging in a cohort of young healthy carriers of the BRCA mutation. We hypothesized that the role played by BRCA genes in aging pathways is not exclusive to the ovary. Experimental Design: Healthy female BRCA carriers, 40 years or younger and healthy male BRCA carriers, 50 years or younger, were enrolled in the study...
March 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29659191/optimised-arid1a-immunohistochemistry-is-an-accurate-predictor-of-arid1a-mutational-status-in-gynaecological-cancers
#6
Saira Khalique, Kalnisha Naidoo, Ayoma D Attygalle, Divya Kriplani, Frances Daley, Anne Lowe, James Campbell, Thomas Jones, Michael Hubank, Kerry Fenwick, Nicholas Matthews, Alistair G Rust, Christopher J Lord, Susana Banerjee, Rachael Natrajan
ARID1A is a tumour suppressor gene that is frequently mutated in clear cell and endometrioid carcinomas of the ovary and endometrium and is an important clinical biomarker for novel treatment approaches for patients with ARID1A defects. However, the accuracy of ARID1A immunohistochemistry (IHC) as a surrogate for mutation status has not fully been established for patient stratification in clinical trials. Here we tested whether ARID1A immunohistochemistry could reliably predict ARID1A mutations identified by next-generation sequencing...
April 16, 2018: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/29615536/protective-effects-of-melatonin-against-nicotine-induced-disorder-of-mouse-early-folliculogenesis
#7
Yu-Feng Wang, Xiao-Feng Sun, Ze-Li Han, Lan Li, Wei Ge, Yong Zhao, Massimo De Felici, Wei Shen, Shun-Feng Cheng
In this paper, we show that neonatal mice injected for five consecutive days with nicotine display impaired germ cell cyst breakdown and primordial follicle assembly resulting in decreased ovarian reserve lasting until sex maturation age. The effects of nicotine on the pups ovaries were associated with decreased expression of oocyte specific genes such as Nobox , Lhx8 , Figlα and Sohlh2 . Moreover, the ovaries of pups injected with nicotine showed increased level of cell oxidative stress and autophagic markers (upregulation of AMPKα-1, increased ratio LC3-II/LC3-I, downregulation of AKT and mTOR)...
March 28, 2018: Aging
https://www.readbyqxmd.com/read/29603726/mechanisms-driving-the-lactate-switch-in-chinese-hamster-ovary-cells
#8
REVIEW
Fiona Hartley, Tracy Walker, Vicky Chung, Karl Morten
The metabolism of Chinese Hamster Ovary (CHO) cells in a production environment has been extensively investigated. However, a key metabolic transition, the switch from lactate production to lactate consumption, remains enigmatic. Though commonly observed in CHO cultures, the mechanism(s) by which this metabolic shift is triggered is unknown. Despite this, efforts to control the switch have emerged due to the association of lactate consumption with improved cell growth and productivity. This review aims to consolidate current theories surrounding the lactate switch...
March 31, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29601583/chronic-restraint-stress-induces-excessive-activation-of-primordial-follicles-in-mice-ovaries
#9
Minhua Xu, Junyan Sun, Qian Wang, Qiuwan Zhang, Chunsheng Wei, Dongmei Lai
Chronic stress is an important factor influencing people's health. It usually causes endocrinal disorders and a decline in reproduction in females. Although studies of both human and animals suggest a detrimental effect of stress on reproduction, the influence of chronic stress on the ovarian reservation and follicular development is still not clear. In this study, a chronic restraint stress (CRS) mouse model was used to investigate the effect of stress on ovarian reservation and follicular development and explore the underlying mechanism...
2018: PloS One
https://www.readbyqxmd.com/read/29589294/examination-of-the-ovotoxicity-of-5-fluorouracil-in-mice
#10
M Lambouras, S H Liew, K Horvay, H E Abud, J M Stringer, Karla J Hutt
PURPOSE: Undesirable side effects of cancer treatments are common and include damage to the ovary, and depletion of the follicle reserve, which if severe enough, can lead to infertility and early menopause. Antimetabolite drugs, such as 5-fluorouracil (5-FU), are not considered to be detrimental to the ovary, but the ovotoxicity of 5-FU has not been evaluated in any detail. The purpose of this study was to evaluate the effects of 5-FU on follicle number. METHODS: In this study, adult female C57Bl6 mice (n = 4-6 animals/group) received a single dose of saline or 5-FU (150 mg/kg) and markers of ovarian damage and follicle depletion were assessed 12 h and 7 days later...
March 27, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29566278/down-regulation-of-poteg-predicts-poor-prognosis-in-esophageal-squamous-cell-carcinoma-patients
#11
Ling Wang, Mengqing Li, Yuting Zhan, Xiaojiao Ban, Tingting Zeng, Yinghui Zhu, Jingping Yun, Xin-Yuan Guan, Yan Li
POTE ankyrin domain family, member G (poteg) belongs to POTE family. The POTE family is composed of many proteins which are very closely related and expressed in prostate, ovary, testis, and placenta. Some POTE paralogs are related with some cancers. Here we showed that down-regulation of POTEG was detected in about 60% primary esophageal squamous cell carcinoma (ESCC) tumor tissues. Clinical association studies determined that POTEG down-regulation was significantly correlated with tumor differentiation, lymph nodes metastasis and TNM staging...
March 22, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29564948/the-effects-of-sclerotherapy-with-5-trichloroacetic-acid-on-the-cyst-diameter-and-ovarian-tissue-in-the-rat-ovarian-cyst-model
#12
Gökhan Artaş, Şehmus Pala, Tuncay Kuloğlu, Remzi Atilgan, Şeyda Yavuzkir, Alparslan Akyol
The aim of this study was to compare the effects of only aspiration with aspiration and 5% trichloroacetic acid (TCA) application on ovarian cyst size and ovarian reserve. The ovarian cysts of 14 rats that were divided into two groups randomly were investigated after total salpingectomy procedure. G1 was the group of saline application after cyst aspiration, while in G2, after aspiration 5% TCA at half amount of aspiration volume was injected into the cyst and re-aspirated after five minutes. The abdomens of the rats were closed and re-explored after 1 month...
March 22, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29540854/22q11-2-rearrangements-found-in-women-with-low-ovarian-reserve-and-premature-ovarian-insufficiency
#13
Sylvie Jaillard, Elena J Tucker, Linda Akloul, Marion Beaumont, Mathilde Domin, Laurent Pasquier, Guilhem Jouve, Sylvie Odent, Marc-Antoine Belaud-Rotureau, Célia Ravel
Ovarian reserve represents the number of available follicles/oocytes within ovaries and it can be assessed by follicle stimulating hormone levels, anti-Müllerian hormone levels, and/or antral follicle count determined by ultrasounds. A low ovarian reserve is defined by an abnormal ovarian reserve test. This condition can be considered premature if it occurs before the age of 40, leading to premature ovarian insufficiency. Despite the growing knowledge concerning the genetic basis of ovarian deficiency, the majority of cases remain without a genetic diagnosis...
March 14, 2018: Journal of Human Genetics
https://www.readbyqxmd.com/read/29534229/ceramide-1-phosphate-has-protective-properties-against-cyclophosphamide-induced-ovarian-damage-in-a-mice-model-of-premature-ovarian-failure
#14
Natalia Pascuali, Leopoldina Scotti, Mariana Di Pietro, Gonzalo Oubiña, Diana Bas, María May, Antonio Gómez Muñoz, Patricia S Cuasnicú, Débora J Cohen, Marta Tesone, Dalhia Abramovich, Fernanda Parborell
STUDY QUESTION: Is ceramide-1-phosphate (C1P) an ovarian protective agent during alkylating chemotherapy? SUMMARY ANSWER: Local administration of C1P drastically reduces ovarian damage induced by cyclophosphamide (Cy) via protection of follicular reserve, restoration of hormone levels, inhibition of apoptosis and improvement of stromal vasculature, while protecting fertility, oocyte quality and uterine morphology. WHAT IS KNOWN ALREADY: Cancer-directed therapies cause accelerated loss of ovarian reserve and lead to premature ovarian failure (POF)...
March 9, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29525687/ovarian-reserve-in-polycystic-ovary-syndrome-more-but-for-how-long
#15
EDITORIAL
Dominique de Ziegler, Paul Pirtea, Renato Fanchin, Jean Marc Ayoubi
No abstract text is available yet for this article.
March 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29502336/impact-of-ovarian-metastases-on-survival-in-patients-treated-with-cytoreductive-surgery-and-hyperthermic-intraperitoneal-chemotherapy-for-peritoneal-malignancy-of-appendiceal-and-colorectal-cancer-origin
#16
Mark B Bignell, Akash M Mehta, Sue Alves, Kandiah Chandrakumaran, Sanjeev P Dayal, Faheez Mohamed, Tom D Cecil, Brendan J Moran
AIM: Ovarian metastases from gastrointestinal tract malignancy have been considered an ominous finding with poor prognosis. The aim of this project was to determine the impact on survival, and potential cure, when cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) are combined to treat peritoneal malignancy in women with Krukenberg tumours. METHOD: Retrospective analysis of prospectively collected data between January 2010 and July 2015...
March 4, 2018: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/29497951/expression-of-inhibitor-proteins-that-control-primordial-follicle-reserve-decreases-in-cryopreserved-ovaries-after-autotransplantation
#17
Soner Celik, Ferda Topal Celikkan, Sinan Ozkavukcu, Alp Can, Ciler Celik-Ozenci
PURPOSE: Even with 86 live births reported globally so far, the mechanism of primordial follicle loss following autotransplantation of the frozen-thawed ovarian tissue needs further evaluation. Pten, Tsc1, p27, and Amh are the inhibitor proteins that play crucial roles in suppressing the transition from the primordial follicle to primary state, maintaining the primordial follicle reserve. In this study, we aimed to evaluate whether the expression patterns of these proteins change and it may be related to the global primordial follicle loss after autotransplantation of the frozen-thawed ovarian tissue...
March 1, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29486615/first-data-on-in-vitro-fertilization-and-blastocyst-formation-after-intraovarian-injection-of-calcium-gluconate-activated-autologous-platelet-rich-plasma
#18
E Scott Sills, Natalie S Rickers, Xiang Li, Gianpiero D Palermo
Platelets modulate clinically relevant yet incompletely understood tissue regeneration processes, and platelet rich plasma (PRP) has been previously used with some success in various non-reproductive medical contexts. Here, we extended PRP application to ovarian tissue with a view to document impact on ovarian reserve among women attending for infertility treatment. PRP was freshly isolated from patients (n= 4) with diminished ovarian reserve as determined by at least one prior IVF cycle canceled for poor follicular recruitment response or estimated by serum AMH and/or FSH, no menses for ≥1 year...
February 28, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29476810/impact-of-ultrasound-guided-transvaginal-ovarian-needle-drilling-versus-laparoscopic-ovarian-drilling-on-ovarian-reserve-and-pregnancy-rate-in-polycystic-ovary-syndrome-a-randomized-clinical-trial
#19
Mohamed Kandil, Mohamed Rezk, Alaa Al-Halaby, Mohamed Emarh, Ibrahim Saif El-Nasr
STUDY OBJECTIVE: To compare the impact of ultrasound-guided transvaginal ovarian needle drilling (TND) versus laparoscopic ovarian drilling (LOD) on ovarian reserve and pregnancy rate in patients with clomiphene citrate (CC)-resistant polycystic ovary syndrome (PCOS). DESIGN: A randomized clinical trial (Canadian Task Force classification I). SETTING: A university hospital. PATIENTS: Of 644 patients who presented at an infertility clinic, 246 with CC-resistant PCOS were randomized for treatment...
February 21, 2018: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29464875/probing-lactate-metabolism-variations-in-large-scale-bioreactors
#20
Sen Xu, Rubin Jiang, Roland Mueller, Nadja Hoesli, Thomas Kretz, John Bowers, Hao Chen
Lactate metabolism variations are frequently encountered in mammalian cell culture processes, especially during process scale-up. In this study, we took a multipronged approach to investigate the impact of pH, pCO2 , osmolality, base addition, and mixing conditions on the observed lactate variations in a Chinese Hamster Ovary (CHO) fed-batch process at 2,000 L scale. Two cultivating methods, CO2 -controlled and pH-controlled, were used to decouple the individual and synergistic effects from those factors. The individual effects from pH, pCO2 , and osmolality on lactate consumption/reproduction in the stationary phase were insignificant in the ranges studied though the initial lactate production rates varied...
February 21, 2018: Biotechnology Progress
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