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https://www.readbyqxmd.com/read/28822138/light-microscopy-transmission-electron-microscopy-and-immunohistochemistry-protocols-for-studying-photorespiration
#1
Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis, Tammy L Sage
High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822074/general-equations-for-optimal-selection-of-diagnostic-image-acquisition-parameters-in-clinical-x-ray-imaging
#2
Xiaoming Zheng
The purpose of this work was to examine the effects of relationship functions between diagnostic image quality and radiation dose on the governing equations for image acquisition parameter variations in X-ray imaging. Various equations were derived for the optimal selection of peak kilovoltage (kVp) and exposure parameter (milliAmpere second, mAs) in computed tomography (CT), computed radiography (CR), and direct digital radiography. Logistic, logarithmic, and linear functions were employed to establish the relationship between radiation dose and diagnostic image quality...
August 18, 2017: Radiological Physics and Technology
https://www.readbyqxmd.com/read/28822031/advances-in-the-management-of-thyroid-eye-diseases-an-overview
#3
REVIEW
Rashmi Kumari, Bhawesh Chandra Saha
INTRODUCTION: Thyroid eye disease (TED) remains a notorious ailment for both patients and the treating ophthalmologists. Recent years have witnessed considerable research in the immunopathogenic mechanism of TED that has resulted in an expansion and modification of the available management options. AIM: Purpose of this review is to summarise the advances in the management of thyroid ophthalmopathy. MATERIAL AND METHOD: A thorough literature search and of the past 10 years web search with words Thyroid ophthalmopathy, recent, advances...
August 18, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28821920/tspo-pet-for-glioma-imaging-using-the-novel-ligand-18-f-ge-180-first-results-in-patients-with-glioblastoma
#4
Nathalie L Albert, M Unterrainer, D F Fleischmann, S Lindner, F Vettermann, A Brunegraf, L Vomacka, M Brendel, V Wenter, C Wetzel, R Rupprecht, J-C Tonn, C Belka, P Bartenstein, M Niyazi
OBJECTIVE: The 18-kDa mitochondrial translocator protein (TSPO) was reported to be upregulated in gliomas. (18)F-GE-180 is a novel 3rd generation TSPO receptor ligand with improved target-to-background contrast compared to previous tracers. In this pilot study, we compared PET imaging with (18)F-GE-180 and MRI of patients with untreated and recurrent pretreated glioblastoma. METHODS: Eleven patients with histologically confirmed IDH wildtype gliomas (10 glioblastomas, 1 anaplastic astrocytoma) underwent (18)F-GE-180 PET at initial diagnosis or recurrence...
August 19, 2017: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28820580/enhanced-fluorescence-emission-and-singlet-oxygen-generation-of-photosensitizers-embedded-in-injectable-hydrogels-for-imaging-guided-photodynamic-cancer-therapy
#5
Liu-Yuan Xia, Xiaodong Zhang, Meng Cao, Zhan Chen, Fu-Gen Wu
Benefiting from their inherent localized and controlled release properties, hydrogels are ideal delivery systems for therapeutic drugs or nanoparticles. In particular, the applications of hydrogels for the delivery and release of photo-responsive drugs or nanoparticles are receiving increasing attention. However, the effect of the hydrogel matrix on the fluorescence emission and singlet oxygen generation efficiency of the embedded photosensitizers (PSs) has not been clarified. Herein, meso-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP) as a water-soluble PS is encapsulated into an injectable hydrogel formed by glycol chitosan and dibenzaldehyde-terminated telechelic poly(ethylene glycol)...
August 18, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28820310/orbital-mantle-cell-lymphoma-presenting-as-myasthenia-gravis
#6
Justin Karlin, Travis Peck, Karyn Prenshaw, Craig A Portell, Maria Kirzhner
A 69-year-old man, previously treated with pyridostigmine for myasthenia gravis (manifesting as ptosis and diplopia) was evaluated for several concomitant bilateral anterior orbital masses. Imaging revealed 3 discrete, solid masses within and around the orbits. An incisional biopsy demonstrated atypical lymphocytes positive for CD20 and Cyclin-D1, consistent with mantle cell lymphoma. The patient received induction chemotherapy with a rituximab-based regimen. He experienced resolution of his diplopia and ptosis after one cycle of chemotherapy and achieved complete remission of the orbital masses and myasthenia symptoms after 6 cycles...
August 18, 2017: Orbit
https://www.readbyqxmd.com/read/28819658/weld-formation-during-material-extrusion-additive-manufacturing
#7
Jonathan E Seppala, Seung Hoon Han, Kaitlyn E Hillgartner, Chelsea S Davis, Kalman B Migler
Material extrusion (ME) is a layer-by-layer additive manufacturing process that is now used in personal and commercial production where prototyping and customization are required. However, parts produced from ME frequently exhibit poor mechanical performance relative to those from traditional means; moreover, fundamental knowledge of the factors leading to development of inter-layer strength in this highly non-isothermal process is limited. In this work, we seek to understand the development of inter-layer weld strength from the perspective of polymer interdiffusion under conditions of rapidly changing mobility...
August 18, 2017: Soft Matter
https://www.readbyqxmd.com/read/28819458/focused-ultrasound-hyperthermia-mediated-drug-delivery-using-thermosensitive-liposomes-and-visualized-with-in-vivo-two-photon-microscopy
#8
Marc A Santos, David E Goertz, Kullervo Hynynen
The future of nanomedicines in oncology requires leveraging more than just the passive drug accumulation in tumors through the enhanced permeability and retention effect. Promising results combining mild hyperthermia (HT) with lyso-thermosensitive liposomal doxorubicin (LTSL-DOX) has led to improved drug delivery and potent antitumor effects in pre-clinical studies. The ultimate patient benefit from these treatments can only be realized when robust methods of HT can be achieved clinically. One of the most promising methods of non-invasive HT is the use of focused ultrasound (FUS) with MRI thermometry for anatomical targeting and feedback...
2017: Theranostics
https://www.readbyqxmd.com/read/28819093/non-seminomatous-germ-cell-tumor-presenting-with-superior-vena-cava-syndrome
#9
Paolo K Soriano, Muhammad F Iqbal, Omar M Siddiqui, Jeff F Wang, Meghna R Desai
BACKGROUND Primary mediastinal non-seminomatous germ cell tumors (NSGCTs) are aggressive and carry a poor five-year disease free survival rate even with aggressive treatment. We describe a young adult male with primary mediastinal NSGCT presenting with airway obstruction and superior vena cava syndrome (SVCS). CASE REPORT The patient presented with four weeks of nonproductive cough, weight loss, and right-sided pleuritic chest pain. Chest computed topography (CT) imaging demonstrated a right-sided mediastinal mass determined as a yolk sac tumor on biopsy...
August 18, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28818992/long-half-life-89-zr-labeled-radiotracers-can-guide-in-suite-percutaneous-molecular-imaging-pet-ct-guided-biopsies-without-reinjection-of-radiotracer
#10
Francois H Cornelis, Jeremy Durack, Neeta Pandit-Taskar, Gary A Ulaner, Jason S Lewis, Michael J Morris, Stephen B Solomon
Rationale: To evaluate the feasibility of in suite Zr89 labeled radiotracer positron emission tomography-computed tomography (PET/CT)-guided biopsies performed without reinjection. Methods: From 2013-2016, 12 patients (7 males, 5 females; mean age 61 years, range 40-75) with suspected metastatic prostate or breast carcinoma on either imaging or biochemical progression underwent 14 percutaneous biopsies after diagnostic PET/CT using (89)Zr labeled radiotracers (mean dose: 180MBq; range: 126-189MBq) targeting prostate specific membrane antigen (PSMA) (n = 7) or human epidermal growth factor receptor 2 (HER2) (n = 5)...
August 17, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28818983/current-concepts-in-68-ga-dotatate-nen-imaging-interpretation-biodistribution-dosimetry-and-molecular-strategies
#11
Lisa Bodei, Valentina Ambrosini, Ken Herrmann, Irvin Modlin
(68)Ga-DOTATATE PET/CT provides information of the location(s) of somatostatin receptor expressing tumors. Integrating this imaging data effectively in patient care requires the clinical history, the histopathology and biomarker information as well as grade, stage and prior imaging. Previous therapies and technical aspects of the study should be considered, given their ability to alter the interpretation of the images. This includes physiologic biodistribution of the radiotracer, as well as conditions that engender false positive results...
August 17, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28818410/optical-coherence-tomography-guided-percutaneous-coronary-intervention
#12
REVIEW
Francis J Ha, Joel P Giblett, Nitesh Nerlekar, James D Cameron, Ian T Meredith, Nick E J West, Adam J Brown
Optical coherence tomography (OCT) is an increasingly available intracoronary imaging modality that provides high-resolution imaging of coronary arteries. Its fundamental reliance on the emission and reflection of light enables rapid data acquisition without compromise of image resolution. As such, OCT can inform operators planning percutaneous coronary intervention (PCI) by accurately defining luminal geometry and detailing plaque composition. Following PCI, OCT imaging delivers a thorough assessment of the treated arterial segment and can identify specific features not always visible on alternate imaging modalities, including stent edge-related dissection, plaque tissue prolapse, incomplete stent apposition and the presence of intra-coronary thrombus...
August 4, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28818226/percutaneous-ultrasound-guided-sclerotherapy-with-polidocanol-microfoam-for-lymphatic-malformations
#13
Takashi Yamaki, Yumiko Sasaki, Yuki Hasegawa, Atsuyoshi Osada, Hisato Konoeda, Atsumori Hamahata, Motohiro Nozaki, Hiroyuki Sakurai
BACKGROUND: Lymphatic malformations (LMs) are low-flow congenital lesions that consist of cysts of varying size. Sclerotherapy with intralesional bleomycin and OK-432 has been reported to yield dramatically beneficial results for this disorder. However, inflammation-related symptoms are often seen after treatment with these sclerosing agents. On the other hand, polidocanol (POL) is reportedly associated with fewer allergic and inflammatory reactions. Up to now, however, very few reports have documented the use of POL microfoam for treatment of LMs...
September 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28818219/contemporary-outcomes-after-venography-guided-treatment-of-patients-with-may-thurner-syndrome
#14
Johnathon C Rollo, Steven M Farley, Adam Z Oskowitz, Karen Woo, Brian G DeRubertis
OBJECTIVE: Patients with May-Thurner syndrome (MTS) present with a spectrum of findings ranging from mild left leg edema to extensive iliofemoral deep venous thrombosis (DVT). Whereas asymptomatic left common iliac vein (LCIV) compression can be seen in a high proportion of normal individuals on axial imaging, the percentage of these persons with symptomatic compression is small, and debate exists about the optimal clinical and diagnostic criteria to treat these lesions in patients with symptomatic venous disease...
September 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28817802/molecular-memory-of-morphologies-by-septins-during-neuron-generation-allows-early-polarity-inheritance
#15
Leila Boubakar, Julien Falk, Hugo Ducuing, Karine Thoinet, Florie Reynaud, Edmund Derrington, Valérie Castellani
Transmission of polarity established early during cell lineage history is emerging as a key process guiding cell differentiation. Highly polarized neurons provide a fascinating model to study inheritance of polarity over cell generations and across morphological transitions. Neural crest cells (NCCs) migrate to the dorsal root ganglia to generate neurons directly or after cell divisions in situ. Using live imaging of vertebrate embryo slices, we found that bipolar NCC progenitors lose their polarity, retracting their processes to round for division, but generate neurons with bipolar morphology by emitting processes from the same locations as the progenitor...
August 16, 2017: Neuron
https://www.readbyqxmd.com/read/28816872/application-of-aospine-subaxial-cervical-spine-injury-classification-in-simple-and-complex-cases
#16
Bizhan Aarabi, Cumhur Oner, Alexander R Vaccaro, Gregory D Schroeder, Noori Akhtar-Danesh
INTRODUCTION: Cervical spine injury classification systems should be simple, easy to relate and remember, reliable guide for surgical planning, and predictor of outcome in clinical settings. We investigated whether the AOSpine subaxial cervical spine classification system predicted injury severity and neurologic outcome. MATERIAL AND METHODS: We analyzed the relevant clinical, imaging, management, and American Spinal Injury Association (ASIA) impairment scale (AIS) grade conversion of 92 AIS grades A-C patients with cervical spine injury...
September 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28816784/images-in-anesthesiology-ultrasound-guided-intraarticular-knee-injection
#17
Neda Sadeghi, Amanda Kumar, James Kim, Joshua Dooley
No abstract text is available yet for this article.
September 2017: Anesthesiology
https://www.readbyqxmd.com/read/28816147/evaluating-which-dose-function-metrics-are-most-critical-for-functional-guided-radiation-therapy
#18
Austin M Faught, Tokihiro Yamamoto, Richard Castillo, Edward Castillo, Jingjing Zhang, Moyed Miften, Yevgeniy Vinogradskiy
PURPOSE: Four-dimensional (4D) computed tomography (CT) ventilation imaging is increasingly being used to calculate lung ventilation and implement functional-guided radiation therapy in clinical trials. There has been little exhaustive work evaluating which dose-function metrics should be used for treatment planning and plan evaluation. The purpose of our study was to evaluate which dose-function metrics best predict for radiation pneumonitis (RP). METHODS AND MATERIALS: Seventy lung cancer patients who underwent 4D CT imaging and pneumonitis grading were assessed...
September 1, 2017: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/28815905/degradable-vanadium-disulfide-nanostructures-with-unique-optical-and-magnetic-functions-for-cancer-theranostics
#19
Yuyan Chen, Liang Cheng, Ziliang Dong, Yu Chao, Huali Lei, He Zhao, Jian Wang, Zhuang Liu
Multifunctional biodegradable inorganic theranostic nano-agents are of great interests to the field of nanomedicine. Herein, VS2 nanosheets upon lipid modification could be converted into ultra-small VS2 nanodots encapsulated inside polyethylene glycol (PEG) modified lipid micelles. Owing to the paramagnetic property, high near-infrared (NIR) absorbance, and chelator-free 99mTc4+ labeling of VS2, such VS2@lipid-PEG nanoparticles could be used for T1-weighted magnetic resonance (MR), photoacoustic (PA),and single photon emission computed tomography (SPECT) tri-modal imaging guided photothermal ablation of tumors...
August 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28815450/a-case-of-pancreatic-cancer-with-severe-vomiting-treated-by-endoscopic-ultrasound-guided-celiac-ganglia-neurolysis
#20
Asami Kawai, Masataka Kikuyama, Kohei Enokida, Shinya Kawaguchi, Naofumi Shirane, Shuzo Terada
A 50-year-old man with advanced pancreatic cancer was admitted for intractable severe vomiting 5-6 times a day, continuing over a week. He had been treated for advanced pancreatic cancer with chemotherapy for 6 months, and had undergone self-expandable metalic stent placement for obstructive jaundice due to the pancreatic cancer 4 months before admission. No abnormal findings suggesting gastrointestinal obstruction or brain metastasis were revealed on diagnostic imaging. We performed endoscopic ultrasound-guided celiac ganglia neurolysis twice by injecting ethanol into the celiac ganglion...
August 16, 2017: Clinical Journal of Gastroenterology
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