S Niesporek, W Weichert, B Sinn, A Röske, A Noske, A C Buckendahl, R Wirtz, J Sehouli, D Koensgen, M Dietel, C Denkert
AIMS: NF-kappaB has been demonstrated to activate proliferative, inflammatory, and angiogenic processes in ovarian cancer cells in vitro. To add translational information on the situation in vivo, we determined the expression pattern of p65, an important subunit of the classic NF-kappaB pathway, in ovarian carcinoma tissue, and investigated in vivo and in vitro whether this pathway is implicated in the known overexpression of cyclooxygenase-2 (COX-2). METHODS: p65 siRNA, chemiluminescent NF-kappaB transcription factor assay, Taqman PCR, as well as immunoblotting were performed with OVCAR-3 ovarian cancer cells...
2007: Verhandlungen der Deutschen Gesellschaft Für Pathologie