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Prabhaker Yadav, Ratnesh K Tripathi, Rajeev K Singh, Vindhya Mohindra
During the investigation of genes involved in the hypoxia tolerance, novel transcript, Replication Termination Factor 2 homologue (RTF2h), was found to be differentially expressed in brain of Clarias magur (previous name, C. batrachus) whose function was still undefined. Thus, present study was aimed to examine the transcriptional response of novel RTF2h gene, for its possible involvement in hypoxia tolerance in C. magur. Novel transcripts expressed under hypoxic stress were identified from ESTs obtained from SSH libraries of C...
October 14, 2016: Molecular Biology Reports
Vindhya Mohindra, Ratnesh Kumar Tripathi, Akanksha Singh, Ruchi Patangia, Rajeev Kumar Singh, Kuldeep Kumar Lal, Joy Krushna Jena
The Indian catfish, Clarias magur is an air breathing fish, inhabitant of aquatic bodies characterized by low dissolved oxygen levels. It is exposed to hypoxic conditions in its natural habitat. Thus, it can be useful model to study the mechanism of hypoxia stress tolerance. In C. magur, molecular processes facilitating its adaptation to hypoxia stress remain largely unexplored, in part due to unavailability of genomic resources. The suppression subtractive hybridization technique (SSH) was employed to compare the differential expression of transcripts under experimental hypoxic conditions, to that of normoxic conditions...
October 11, 2016: Fish & Shellfish Immunology
Raju Murugananthkumar, Yarikipati Prathibha, Balasubramanian Senthilkumaran, Anbazhagan Rajakumar, Hirohiko Kagawa
Gonadal maturation is a critical event wherein gonads, under the influence of several hormones and factors, undergo cyclic morphological and physiological changes to produce functional gametes during the spawning phase. However, artificial induction can be effectively used to advance the maturation of gonad vis-à-vis spawning like behavior in seasonal breeders during the off-breeding season. In the present study, osmotic pumps loaded with 5000IU of human chorionic gonadotropin (hCG) or saline as control were implanted intraperitoneally for 21days during the pre-spawning phase (May-June) in catfish Clarias batrachus and C...
October 5, 2016: General and Comparative Endocrinology
Felipe R Blasco, David J McKenzie, Edwin W Taylor, F Tadeu Rantin
Clarias gariepinus is a facultative air-breathing catfish that exhibits changes in heart rate (ƒH) associated with air-breaths (AB). A transient bradycardia prior to the AB is followed by sustained tachycardia during breath-hold. This study evaluated air-breathing and cardiac responses to sustained aerobic exercise in juveniles (total length ~20 cm), and how exercise influenced variations in fH associated with AB. In particular, it investigated the role of adrenergic and cholinergic control in cardiac responses, and effects of pharmacological abolition of this control on air-breathing responses...
October 5, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
J E Barasa, R Abila, J P Grobler, M Agaba, E J Chemoiwa, B Kaunda-Arara
In order to improve the conservation and sustainable utilization of the African catfish Clarias gariepinus of the Yala Swamp in Kenya, genetic diversity and population structure of Lakes Kanyaboli and Namboyo populations of the species were studied using DNA sequences of the mitochondrial D-loop control region. Genetic diversity inferred as haplotype and nucleotide diversities and number of singletons and shared haplotypes was higher in the Lake Kanyaboli population (LKG) than the Lake Namboyo population (LNG) of C...
September 29, 2016: Journal of Fish Biology
Lutz Ahrens, Habiba Gashaw, Margareta Sjöholm, Solomon Gebreyohannis Gebrehiwot, Abebe Getahun, Ermias Derbe, Kevin Bishop, Staffan Åkerblom
Lake Tana is Ethiopia's largest lake and there are plans to increase the harvest of fish from the lake. The objective of this study was to assess the levels of poly- and perfluoroalkyl substances (PFASs) in different compartments of the lake (water, sediment, and fish muscle tissue), and its implications for human exposure. The results showed higher PFAS concentrations in piscivorous fish species (Labeobarbus megastoma and Labeobarbus gorguari) than non-piscivorous species (Labeobarbus intermedius, Oreochromis niloticus and Clarias gariepinus) and also spatial distribution similarities...
December 2016: Chemosphere
Shreya Srivastava, Basdeo Kushwaha, Jyoti Prakash, Ravindra Kumar, N S Nagpure, Suyash Agarwal, Manmohan Pandey, P Das, C G Joshi, J K Jena
Indian magur (Clarias batrachus) is an important freshwater catfish, which is listed as endangered under A3cde+4acde ver. 3.1 categories by the IUCN (2015) due to decreasing population trend. Microsatellites or short sequence repeats (SSRs) tagged to genes have been utilized as gene marker. In the present study, 31,814 SSRs of C. batrachus (magur) were identified using microsatellite identification tool programme from the next generation sequencing data generated on Roche 454 and Ion Torrent platforms. A bioinformatics pipeline, with stringent criteria resulted in selection of 1672 microsatellite loci falling in the genic region...
September 2016: Journal of Genetics
Esther Titos, Bibiana Rius, Cristina López-Vicario, José Alcaraz-Quiles, Verónica García-Alonso, Aritz Lopategi, Jesmond Dalli, Juan José Lozano, Vicente Arroyo, Salvadora Delgado, Charles N Serhan, Joan Clària
Persistent activation of the innate immune system greatly influences the risk for developing metabolic complications associated with obesity. In this study, we explored the therapeutic potential of the specialized proresolving mediator (SPM) resolvin D1 (RvD1) to actively promote the resolution of inflammation in human visceral adipose tissue from obese (Ob) patients. Using liquid chromatography-tandem mass spectrometry-based metabololipidomic analysis, we identified unbalanced production of SPMs (i.e., D- and E-series resolvins, protectin D1, maresin 1, and lipoxins) with respect to inflammatory lipid mediators (i...
September 19, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
M I Abd El-Hamid, N K Abd El-Aziz, H A Ali
Disease episodes of fish caused by Aeromonas species are moved to the top list of limiting problems worldwide. The present study was planned to verify the in vitro antibacterial activities as well as the in vivo potential values of clove oil and ciprofloxacin against Aeromonas sobria in African catfish (Clarias gariepinus). The in vitro phenotypic virulence activities and the successful amplification of aerolysin and hemolysin genes in the precisely identified A. sobria strain were predictive for its virulence...
2016: Cellular and Molecular Biology
Usha Kumari, Nidhi Srivastava, Asha Shelly, Preeti Khatri, Sarat N, Dileep Kumar Singh, Shibnath Mazumder
The effect of endosulfan metabolites on fish immune system is not well known. It is also not clear whether endosulfan accumulates in fish immune organs and undergoes metabolic biotransformation in situ. In the present study we investigated the role of headkidney (HK), an important fish immune organ on endosulfan metabolism and the long term effects of endosulfan metabolites on the fish immune system. C. gariepinus (walking catfish) were exposed to 2.884ppb of endosulfan (1/10th LC50) for 30d followed by their maintenance in endosulfan-free water for 30d for recovery...
October 2016: Aquatic Toxicology
Aina O Adeogun, Azubuike V Chukwuka, Chukwunonso P Okoli, Augustine Arukwe
The distributions of polychlorinated biphenyl (PCB) congeners were determined in sediment and muscle of the African sharptooth catfish (Clarias gariepinus) from the Ogun and Ona rivers, southwest Nigeria. In addition, the effect of PCB congeners on condition factor (CF) and associated human health risk was assessed using muscle levels for a noncarcinogenic hazard quotient (HQ) calculation. Elevated concentrations of high-molecular-weight (HMW) PCB congeners were detected in sediment and fish downstream of discharge points of both rivers...
2016: Journal of Toxicology and Environmental Health. Part A
Ali Karami, Dzolkhifli Omar, James M Lazorchak, Chee Kong Yap, Zailina Hashim, Simon C Courtenay
Influence of waterborne butachlor (BUC), a commonly used pesticide, on morphometric, biochemical, and molecular biomarkers was evaluated in juvenile, full sibling, diploid and triploid African catfish (Clarias gariepinus). Fish were exposed for 21 days to one of three concentrations of BUC [mean measured µg/L: 22, 44 or 60]. Unexposed (control) triploids were heavier and longer and had higher visceral-somatic index (VSI) than diploids. Also, they had lighter liver weight (HSI) and showed lower transcript levels of brain gonadotropin-releasing hormone (GnRH), aromatase (cyp191b) and fushi tarazu-factor (ftz-f1), and plasma testosterone levels than diploids...
August 11, 2016: Environmental Research
Mustafa Tunçsoy, Servet Duran, Özcan Ay, Bedii Cicik, Cahit Erdem
Accumulation of copper (Cu), applied singly and in mixture with chitosan (CT), in gill, liver, spleen, kidney and muscle tissues of Clarias gariepinus was studied after exposing the fish to no Cu (control), 5 ppm Cu, 5 ppm Cu + 75 ppm CT mixture over 1, 7 and 15 days. Metal levels in tissues were determined using an ICP-AES spectrophotometer. No mortality was observed during the experiments. Highest accumulation of Cu was observed in liver while lowest accumulation was observed in muscle tissue. Exposure to Cu-CT mixture decreased Cu accumulation in liver at all exposure periods and in kidney on the 15th day compared to Cu alone...
October 2016: Bulletin of Environmental Contamination and Toxicology
Rewaida Abdel-Gaber, Thabet Sakarn, Gamal El-Shahawi, Hoda El-Fayoumi, Rana Mohammed
Sixty specimens of African sharptooth catfish Clarias gariepinus (Clariidae) were collected during the period of March 2014-February 2015 from boat landing sites and fishermen of the River Nile at Beni Suef Governorate, Egypt, and examined for the presence of digenean parasites. The morphology of these parasites was studied by using light microscopy to describe morphological and morphometrically measurements for different body parts. Three digenean species belonging to three different genera were collected from various organs within the examined fish species...
November 2016: Parasitology Research
Christopher Didigwu Nwani, Peace Onas Somdare, Emmanuel Okechukwu Ogueji, Jacinta Chinedu Nwani, Juliana Adimonye Ukonze, Alfreda O Nwadinigwe
Fenthion is one of the most widely used organophosphate insecticides for the control of many varieties of pests in Nigeria. The genotoxic effect of the pesticide was evaluated in the blood erythrocytes of Clarias gariepinus using the micronucleus (MN) test. The oxidative stress parameters were also studied in the liver and gill tissues. Fish were exposed to 2.0, 4.0, and 8.0 mgL(-1) of fenthion and sampling was done on days 1, 7, 14, 21 and after 7-day recovery. Micronuclei induction was highest (7.55) on day 14 at all concentrations in the peripheral blood cells...
July 26, 2016: Drug and Chemical Toxicology
Moges Beletew, Abebe Getahun, Maarten P M Vanhove
BACKGROUND: Lake Tana is the largest lake in Ethiopia and the source of the Blue Nile. The lake harbours unique endemic cyprinid fish species, as well as the commercially important endemic Nile tilapia subspecies Oreochromis niloticus tana and the North African catfish Clarias gariepinus. Its endemicity, especially within the Labeobarbus radiation, its conservation importance and its economic indispensability attract scientific interest to the lake's ichthyofauna. Fish parasites of Lake Tana, however, are hitherto poorly known, and no formal report exists on its monogenean flatworms...
2016: Parasites & Vectors
Ali Karami, Nicholas Romano, Tamara Galloway, Hazilawati Hamzah
Despite the ubiquity of microplastics (MPs) in aquatic environments and their proven ability to carry a wide variety of chemicals, very little is known about the impacts of virgin or contaminant-loaded MPs on organisms. The primary aim of this study was to investigate the impacts of virgin or phenanthrene (Phe)-loaded low-density polyethylene (LDPE) fragments on a suite of biomarker responses in juvenile African catfish (Clarias gariepinus). Virgin LDPE (50 or 500µg/L) were preloaded with one of two nominal Phe concentrations (10 or 100µg/L) and were exposed to the fish for 96h...
July 21, 2016: Environmental Research
Swapnarani Nayak, Shajahan Ferosekhan, Sangram Ketan Sahoo, Jitendra Kumar Sundaray, Pallipuram Jayasankar, Hirak Kumar Barman
Spermatogenesis is a highly co-ordinated and complex process. In vitro propagation of spermatogonial stem cells (SSCs) could provide an avenue in which to undertake in vivo studies of spermatogenesis. Very little information is known about the SSC biology of teleosts. In this study, collagenase-treated testicular cells of farmed catfish (Clarias batrachus, popularly known as magur) were purified by Ficoll gradient centrifugation followed by magnetic activated cell sorting using Thy1.2 (CD90.2) antibody to enrich for the spermatogonial cell population...
July 15, 2016: Zygote: the Biology of Gametes and Early Embryos
Gihan G Moustafa, F E Shaaban, A H Abo Hadeed, Walaa M Elhady
AIM: The current study was directed to investigate the immunotoxic and oxidative stress effects of Roundup and Stomp herbicides and their combination on Nile catfish (Clarias gariepinus). MATERIALS AND METHODS: The experiment was carried out on 120 fish that randomly divided into four equal groups with three replicates: The first group kept as control, the second group exposed to 1/2 96 h lethal concentration 50 (LC50) of Roundup, the third group exposed to 1/2 96 h LC50 of Stomp, and the fourth one exposed to a combination of Roundup and Stomp at previously-mentioned doses...
June 2016: Veterinary World
M R Zandomeni, M Chahdi Beltrame, M de Santibañes, O Mazza, J Pekolj, E de Santibañes, R Sanchez Claria
No abstract text is available yet for this article.
July 4, 2016: Journal of Gastrointestinal Cancer
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