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https://www.readbyqxmd.com/read/28718783/metallothionein-from-wild-populations-of-the-african-catfish-clarias-gariepinus-from-sequence-protein-expression-and-metal-binding-properties-to-transcriptional-biomarker-of-metal-pollution
#1
Ethel M'kandawire, Agnieszka Mierek-Adamska, Stephen R Stürzenbaum, Kennedy Choongo, John Yabe, Maxwell Mwase, Ngonda Saasa, Claudia A Blindauer
Anthropogenic pollution with heavy metals is an on-going concern throughout the world, and methods to monitor release and impact of heavy metals are of high importance. With a view to probe its suitability as molecular biomarker of metal pollution, this study has determined a coding sequence for metallothionein of the African sharptooth catfish Clarias gariepinus. The gene product was recombinantly expressed in Escherichia coli in presence of Zn(II), Cd(II), or Cu, and characterised by Electrospray Ionisation Mass Spectrometry and elemental analysis...
July 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28713009/aeromonas-hydrophila-induced-alterations-in-cytosolic-calcium-activate-pro-apoptotic-cpkc-mek1-2-tnf%C3%AE-axis-in-infected-headkidney-macrophages-of-clarias-gariepinus
#2
Asha Shelly, Chaitali Banerjee, Gunjan Kumar Saurav, Atish Ray, Vipin Singh Rana, Rajagopal Raman, Shibnath Mazumder
Alterations in intracellular-calcium (Ca(2+))i homeostasis is critical to Aeromonas hydrophila-induced headkidney macrophages (HKM) apoptosis of Clarias gariepinus, though the implications are poorly understood. Here, we describe the role of intermediate molecules of Ca(2+-)signaling pathway that are involved in HKM apoptosis. We observed phosphoinositide-3-kinase/phospholipase C is critical for (Ca(2+))i release in infected HKM. Heightened protein kinase-C (PKC) activity and phosphorylation of MEK1/2-ERK1/2 was noted which declined in presence of 2-APB, Go6976 and PD98059, inhibitors to IP3-receptor, conventional PKC isoforms (cPKC) and MEK1/2 respectively implicating Ca(2+)/cPKC/MEK-ERK1/2 axis imperative in A...
July 13, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28711705/bmp15-in-catfish-testis-cellular-distribution-seasonal-variation-and-its-role-in-steroidogenesis
#3
Himanshu Yadav, Bechan Lal
Considering the absence of information on testicular growth factors in fishes, present study was aimed to elucidate the existence of BMP15, an important member of TGF-β superfamily, in the testis of a seasonally breeding freshwater catfish, Clarias batrachus and its role in regulation of testicular activities. The study demonstrated the expression of BMP15 in the somatic cells (Sertoli and interstitial cells) in fish testis. The expression varied with changing testicular activity; the expression was very high in the quiescent and early recrudescing testis coinciding with the renewal of spermatogonial cells...
July 12, 2017: Steroids
https://www.readbyqxmd.com/read/28710990/biomarkers-of-oxidative-stress-and-health-risk-assessment-of-heavy-metal-contaminated-aquatic-and-terrestrial-organisms-by-oil-extraction-industry-in-ogale-nigeria
#4
Sunny O Abarikwu, Eka B Essien, Oreva-Oghene Iyede, Kingsley John, Chidimma Mgbudom-Okah
Ogale community in Rivers State, Nigeria is characterized by crude-oil contamination of its land resources. The present study aimed to evaluate the health risk and metal (Cd, Pb, Zn, Cr, and Ni) contamination level of the vegetable (Telfairia occidentalis), snail (Achatina achatina) and the catfish (Clarias gariepinus) collected from Ogale community. Samples collected from Elele Alimini community, a less polluted area was used as control. Oxidative damage was evaluated in tissues of snail and in the liver of catfish...
July 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/28699354/drug-mediated-intracellular-donation-of-nitric-oxide-potently-inhibits-5-lipoxygenase-a-possible-key-to-future-anti-leukotriene-therapy
#5
Jessica Roos, Marcus Peters, Isabelle V Maucher, Benjamin Kühn, Jasmin Fettel, Nadine Hellmuth, Camilla Brat, Benita Sommer, Anja Urbschat, Matthias Piesche, Anja Vogel, Ewgenij Proschak, René Blöcher, Estel la Buscato, Ann-Kathrin Häfner, Carmela Matrone, Oliver Werz, Juliana Heidler, Ilka Wittig, Carlo Angioni, Gerd Geisslinger, Michael J Parnham, Kai Zacharowski, Dieter Steinhilber, Thorsten Jürgen Maier
AIMS: 5-lipoxygenase (5-LO) is the key enzyme of leukotriene biosynthesis and is critically involved in a number of inflammatory diseases such as arthritis, gout, bronchial asthma, atherosclerosis and cancer. Because 5-LO contains critical nucleophilic amino acids, which are sensitive to electrophilic modifications, we determined the consequences of a drug-mediated intracellular release of nitric oxide (NO) on 5-LO product formation by human granulocytes and on 5-LO-dependent pulmonary inflammation in vivo...
July 12, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28666831/steroidogenic-acute-regulatory-protein-star-gene-expression-construct-development-nanodelivery-and-effect-on-reproduction-in-air-breathing-catfish-clarias-batrachus
#6
Pravesh Kumar Rathor, Irfan Ahmad Bhat, Mohd Ashraf Rather, Pathakota Gireesh-Babu, Kundan Kumar, Suresh Babu Padinhate Purayil, Rupam Sharma
Steroidogenic acute regulatory protein (StAR) is responsible for the relocation of cholesterol across mitochondrial membrane in vertebrates and is, therefore, a key factor in regulating the rate and timing of steroidogenesis. In the present study, we developed chitosan nanoparticle (CNP) conjugated StAR gene construct (CNP-pcDNA4-StAR) in a eukaryotic expression vector, pcDNA4/HisMax A. CNPs of 135.4nm diameter, 26.7mV zeta potential and 0.381 polydispersity index were used for conjugation. The loading efficiency (LE) of pcDNA4-StAR construct with CNPs was found to be 86%...
June 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28655659/fungal-chitosan-and-lycium-barbarum-extract-as-anti-listeria-and-quality-preservatives-in-minced-catfish
#7
Mohammed S Alsaggaf, Shaaban H Moussa, Nihal M Elguindy, Ahmed A Tayel
Listeria monocytogenes is a foodborne bacterial pathogen that causes serious health risks. Chitosan (Ch) is a bioactive polymer that could be effectively applied for foodstuffs biopreservation. Lycium barbarum (Goji berry) is ethnopharmaceutical fruit that have diverse health protecting benefits. Chitosan was produced from A. niger and employed with L. barbarum extract (LBE) as blends for Listeria control and quality biopreservation of African catfish mince (Clarias gariepinus). Chitosan could utterly control L...
June 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28635581/first-report-on-the-successful-hybridization-of-pangasianodon-hypophthalmus-sauvage-1878-and-clarias-gariepinus-burchell-1822
#8
V T Okomoda, I C C Koh, M S Shahreza
Breeding and larval performance of novel hybrids from reciprocal crosses of Asian catfish Pangasianodon hypophthalmus (Sauvage, 1878) and African catfish Clarias gariepinus (Burchell, 1822) were investigated in this study. Spawning was by hormonal injection of brood fish, artificial fertilization, and incubation in triplicate aquarium tanks (0.5 × 0.5 × 0.5 m3) with continuous aeration. Reciprocal crosses (♀C. gariepinus × ♂P. hypophthalmus and ♀P. hypophthalmus × ♂C. gariepinus) had lower hatchability (≤50%) than their pure siblings (≥75%)...
June 21, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28631588/spawning-performance-of-clarias-gariepinus-burchell-1822-induced-with-ethanol-preserved-and-fresh-catfish-pituitary-extract
#9
V T Okomoda, L O Tiamiyu, D Kwaghger
This study was conducted to determine the efficacy of preserved and fresh pituitary extract of Clarias gariepinus (Burchell, 1822) to induced spawning in the same species. Growth performance of fry was also monitored for 15 days to determine the possible effect of natural hormone treatment on the fry. Pituitary glands (PG) were obtained from 12 male broodstocks, six extracted PG were preserved in 96% ethanol 24 h before injection, while the other were extracted and used in their fresh state. The PG solutions (1 g ml-1 of saline water) were injected at 1 ml kg-1 of the female broodstock...
June 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28577421/season-dependent-effects-of-photoperiod-and-temperature-on-circadian-rhythm-of-arylalkylamine-n-acetyltransferase2-gene-expression-in-pineal-organ-of-an-air-breathing-catfish-clarias-gariepinus
#10
Kshetrimayum Manisana Singh, Saurav Saha, Braj Bansh Prasad Gupta
Arylalkylamine N-acetyltransferase (AANAT) activity, aanat gene expression and melatonin production have been reported to exhibit prominent circadian rhythm in the pineal organ of most species of fish. Three types of aanat genes are expressed in fish, but the fish pineal organ predominantly expresses aanat2 gene. Increase and decrease in daylength is invariably associated with increase and decrease in temperature, respectively. But so far no attempt has been made to delineate the role of photoperiod and temperature in regulation of the circadian rhythm of aanat2 gene expression in the pineal organ of any fish with special reference to seasons...
May 27, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28554489/hepatoprotective-efficacy-of-spirulina-platensis-against-lead-induced-oxidative-stress-and-genotoxicity-in-catfish-clarias-gariepinus
#11
Alaa El-Din H Sayed, Yasser S El-Sayed, Ali H El-Far
Lead (Pb) is a toxic environmental pollutant that induces a broad range of biochemical and physiological hazards in living organisms. We investigated the possible hepatoprotective effects of Spirulina platensis (SP) in counteracting the Pb-induced oxidative damage. Ninety-six adult African catfish were allocated into four equal groups. The 1st group (control) fed basal diet while the 2nd group (Pb-treated) fed on basal diet and exposed to 1mg Pb(NO3)2/L. The 3rd and 4th groups fed SP-supplemented basal diets at levels of 0...
May 27, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28536798/pollutants-induced-oxidative-stress-dna-damage-and-cellular-deformities-in-clarias-gariepinus-burchell-from-river-yamuna-in-delhi-region-india
#12
Anil K Tyor, Kanika Pahwa
The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR's and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0...
July 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28527047/dietary-ascorbic-acid-reduced-micronucleus-and-nuclear-abnormalities-in-clarias-gariepinus-burchell-1822-exposed-to-hospital-effluent
#13
Chibuisi G Alimba, Raphael D Ajiboye, Olakunle S Fagbenro
Hospital effluents contain myriad of mutagens and genotoxins capable of increasing DNA damage in aquatic biota. African mudfish, Clarias gariepinus, are exposed to genotoxins when cultured in swamps and derelict water bodies often contaminated by effluents. Moreover, its DNA is susceptible to xenobiotic-induced lesions since it lacks L-gulonolactone oxidase and hence cannot synthesize L-ascorbic acid. This study investigated 96-h acute toxicity and protective effects of dietary ascorbic acid (AA) against micronucleus (MN) and abnormal nuclear (NAs) formation in C...
May 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28503260/dynamics-of-hexavalent-chromium-in-four-types-of-aquaculture-ponds-and-its-effects-on-the-morphology-and-behavior-of-cultured-clarias-gariepinus-burchell-1822
#14
Moshood Keke Mustapha
Hexavalent chromium is a bio accumulative toxic metal in water and fish. It enters aquaculture ponds mainly through anthropogenic sources. Hexavalent chromium concentrations and its effects on the morphology and behavior of Clarias gariepinus were investigated from four aquaculture ponds for 12 weeks. Chromium was measured using diphenyl carbohdrazide method; alkalinity and hardness were measured using colometric method and analyzed with Bench Photometer. Temperature and pH were measured using pH/EC/TDS/Temp combined tester...
April 2017: Toxicological Research
https://www.readbyqxmd.com/read/28497648/intrahepatic-cholangiojejunostomy-for-complex-biliary-stenosis-after-pediatric-living-donor-liver-transplantation
#15
Fernando A Alvarez, Rodrigo Sanchez Claria, Juan Glinka, Martin de Santibañes, Juan Pekolj, Eduardo de Santibañes, Miguel A Ciardullo
The treatment of biliary stenosis after pediatric LDLT is challenging. We describe an innovative technique of peripheral IHCJ for the treatment of patients with complex biliary stenosis after pediatric LDLT in whom percutaneous treatment failed. During surgery, the percutaneous biliary drainage is removed and a flexible metal stylet is introduced trough the tract. Subsequently, the most superficial aspect of the biliary tree is recognized by palpation of the stylet's round tip in the liver surface. The liver parenchyma is then transected until the bile duct is reached...
August 2017: Pediatric Transplantation
https://www.readbyqxmd.com/read/28484803/a-multivariate-analysis-of-metal-concentrations-in-two-fish-species-of-the-nyl-river-system-limpopo-province-south-africa
#16
R Musa, R Gerber, R Greenfield
Increased toxicity due to high metal concentrations may hinder the ecological integrity of aquatic ecosystems in sustaining life. The non-biodegradable nature of metals may result in bioaccumulation in aquatic organisms. Due to ecological and social aspects it is imperative for monitoring schemes to identify possible impacts to the systems integrity. This paper discusses accumulation patterns of seven metals (Al, Cr, Cu, Fe, Mn, Sr and Zn) in tissues of two fish species, namely Clarias gariepinus and Oreochromis mossambicus at two selected sites, the first located upstream of Modimolle (NRS1), and the second located downstream of the town (NRS2)...
June 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28466449/distribution-characterization-and-human-health-risk-assessment-of-polycyclic-aromatic-hydrocarbons-pahs-in-ovia-river-southern-nigeria
#17
Isioma Tongo, Lawrence Ezemonye, Kingsley Akpeh
The levels and distribution of polycyclic aromatic hydrocarbons (PAHs) in surface water, sediment, and fish (Clarias gariepinus) samples from Ovia River, Southern Nigeria, were evaluated, to assess the contamination profile, dietary intake, and human health risks associated with exposure. Results showed that naphthalene, acenaphthylene, and fluoranthene were the most dominant contaminant in water, sediment, and fish, respectively, with mean concentrations (μg/L) of 3.08, 45.4, and 90.7. Spatial distribution showed high occurrence of PAHs in Ekenwan, the downstream station, for all the matrices...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28431974/unique-mitochondrial-localization-of-arginase-1-and-2-in-hepatocytes-of-air-breathing-walking-catfish-clarias-batrachus-and-their-differential-expression-patterns-under-hyper-ammonia-stress
#18
Bodhisattwa Banerjee, Debaprasad Koner, Priyanka Lal, Nirmalendu Saha
Arginase (ARG) catalyzes the final step of ornithine-urea cycle (OUC) leading to a conversion of L-arginine to L-ornithine and urea. Several isoforms of ARG have been reported in vertebrates, out of which the two predominant isoforms are the cytosolic ARG1 and the mitochondrial ARG2. The air-breathing walking catfish (Clarias batrachus) is frequently being challenged by different environmental insults such as hyper-ammonia, dehydration and osmotic stresses in their natural habitats throughout the year. The present study investigated the active presence of ARG1 and ARG2 isoforms in hepatocytes along with unique localization of both the isoforms inside the mitochondria, and also their specific expression patterns under hyper-ammonia stress (5mM NH4Cl) in isolated hepatocytes of walking catfish...
July 30, 2017: Gene
https://www.readbyqxmd.com/read/28341541/pro-resolving-actions-of-spm-in-adipose-tissue-biology
#19
REVIEW
Joan Clària, Cristina López-Vicario, Bibiana Rius, Esther Titos
White adipose tissue plays an important endocrine role in balancing metabolic homeostasis. During conditions of nutrient excess, as occurs in obesity, there is an expansion of adipose tissue mass associated with a state of "low-grade" inflammation in this tissue. This chronic, unresolved inflammation of adipose tissue is deleterious and leads to many pathological sequelae associated with obesity including insulin resistance, type 2 diabetes and non-alcoholic fatty liver disease. Recently, a novel genus of anti-inflammatory and pro-resolving lipid mediators endogenously generated from membrane phospholipid-derived polyunsaturated fatty acids has been identified during the resolution phase of acute inflammation...
March 24, 2017: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/28322767/cloning-localization-and-differential-expression-of-neuropeptide-y-during-early-brain-development-and-gonadal-recrudescence-in-the-catfish-clarias-gariepinus
#20
Cheni-Chery Sudhakumari, Arumugam Anitha, Raju Murugananthkumar, Dinesh Kumar Tiwari, Dharavath Bhasker, Balasubramanian Senthilkumaran, Aparna Dutta Gupta
Neuropeptide-Y (NPY) has diverse physiological functions which are extensively studied in vertebrates. However, regulatory role of NPY in relation to brain ontogeny and recrudescence with reference to reproduction is less understood in fish. Present report for the first time evaluated the significance of NPY by transient esiRNA silencing and also analyzed its expression during brain development and gonadal recrudescence in the catfish, Clarias gariepinus. As a first step, full-length cDNA of NPY was cloned from adult catfish brain, which shared high homology with its counterparts from other teleosts upon phylogenetic analysis...
March 17, 2017: General and Comparative Endocrinology
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