Sue C Nang, Jing Lu, Heidi H Yu, Hasini Wickremasinghe, Mohammad A K Azad, Meiling Han, Jinxin Zhao, Gauri Rao, Phillip J Bergen, Tony Velkov, Norelle Sherry, David T McCarthy, Saima Aslam, Robert T Schooley, Benjamin P Howden, Jeremy J Barr, Yan Zhu, Jian Li
OBJECTIVES: Bacteriophage (phage) therapy is a promising anti-infective option to combat antimicrobial resistance. However, the clinical utilisation of phage therapy has been severely compromised by the potential emergence of phage resistance. While certain phage resistance mechanisms can restore bacterial susceptibility to certain antibiotics, a lack of knowledge of phage resistance mechanisms hinders optimal use of phages and their combination with antibiotics. METHODS: Genome-wide transposon screening was performed with a mutant library of Klebsiella pneumoniae MKP103 to identify phage pKMKP103_1-resistant mutants...
March 22, 2024: Clinical Microbiology and Infection